Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F121 I0 R1 856 273.6 9081716 94.1% 8545894 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,395,041 C→A 18.1% R164L (CGG→CTG)  nuoE ← NADH:ubiquinone oxidoreductase, chain E

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,395,0410CA18.1% 709.3 / 116.8 249R164L (CGG→CTG) nuoENADH:ubiquinone oxidoreductase, chain E
Reads supporting (aligned to +/- strand):  ref base C (97/107);  new base A (20/25);  total (117/132)
Fisher's exact test for biased strand distribution p-value = 7.44e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.24e-01

Only 100 of 257 total aligned reads displayed.

TCCGGAGACAGCCCGGTCAGCGCCTTACGCGCGCCTTCGTAACCGTTTTTGCTGCGGTATTCGTCCAGCCACACTGGCTGTTTGTCATCGCGCAGACGCCAGGTCAGCGGATGCGTTTCGGGAGTACGGATAATGTTTTTCATTTATACCGCTCCAGCAGTTCAGGGATCGCTTCCGGGGTCAGATGCGCGTGAGTGTCCTCATCGATCATCATGTTTGGCCCTTTATCACAGTTCCCCAGGCAGCAAGTTGGCAGCAGCGTAAAGCGGCCATCAAATGTCGTTTGCCCTGGTTTGAT  >  CP009273/2394892‑2395189
                                                                                                                                                     |                                                                                                                                                    
tCCGGAGACAGCCCGGTCAGCGCCTTACGCGCGCCTTCGTAACCGTTTTTGCTGCGGTATTCGTCCAGCCACACTGGCTGTTTGTCATCGCGCAGACGCCAGGTCAGCGGATGCGTTTCGGGAGTACGGATAATGTTTTTCATTTATAcc                                                                                                                                                      <  2:1578364/150‑1 (MQ=255)
tCCGGAGACAGCCCGGTCAGCGCCTTACGCGCGCCTTCGTAACCGTTTTTGCTGCGGTATTCGTCCAGCCACACTGGCTGTTTGTCATCGCGCAGACGCCAGGTCAGCGGATGCGTTTCGGGAGTACGGATAATGTTTTTCATTTATAcc                                                                                                                                                      <  2:2217589/150‑1 (MQ=255)
 ccGGAGACAGCCCGGTCAGCGCCTTACGCGCGCCTTCGTAACCGTTTTTGCTGCGGTATTCGTCCAGCCACACTGGCTGTTTGTCATCGCGCAGACGCCAGGTCAGCGGATGCGTTTCGGGAGTACGGATAATGTTTTTCATTTATACCg                                                                                                                                                     >  2:1125794/1‑150 (MQ=255)
 ccGGAGACAGCCCGGTCAGCGCCTTACGCGCGCCTTCGTAACCGTTTTTGCTGCGGTATTCGTCCAGCCACACTGGCTGTTTGTCATCGCGCAGACGCCAGGTCAGCGGATGCGTTTCGGGAGTACGGATAATGTTTTTCATTTATACCg                                                                                                                                                     <  1:3732296/150‑1 (MQ=255)
 ccGGAGACAGCCCGGTCAGCGCCTTACGCGCGCCTTCGTAACCGTTTTTGCTGCGGTAGTCGTCCAGCCACACTGGCTGTTTGTCATCGCGCAGACGCCAGGTCAGCGGATGCGTTTCGGGAGTACGGATAATGTTTTTCATTTATACCg                                                                                                                                                     <  1:47150/150‑1 (MQ=255)
 ccGGAGACAGCCCGGTCAGCGCCTTACGCGCGCCTTCGTAACCGTTTTTGCAGCGGTATTCGTCCAGCCACACTGGCTGTTTGTCATCGCGCAGACGCCAGGTCCGCGGAGGCGATTCGGGAGTACGGATAATCTTTTTCAGTTATACCg                                                                                                                                                     >  2:3616761/1‑150 (MQ=255)
  cGGAGACAGCCCGGTCAGCGCCTTACGCGCGCCTTCGTAACCGTTTTTGCTGCGGTATTCGTCCAGCCACACTGGCTGTTTGTCATCGCGCAGACGCCAGGTCAGCGGATGCGTTTCGGGAGTACGGATAATGTTTTTCATTTATACCGc                                                                                                                                                    >  2:628568/1‑150 (MQ=255)
     agaCAGCCCGGTCAGCGCCTTACGCGCGCCTTCGTAACCGTTTTTGCTGCGGTATTCGTCCAGCCACACTGGCTGTTTGTCATCGCGCAGACGCCAGGTCAGCGGATGCGTTTCGGGAGTACGGATAATGTTTTTCATTTATACCGCTcc                                                                                                                                                 >  2:3227602/1‑150 (MQ=255)
     agaCAGCCCGGTCAGCGCCTTACGCGCGCCTTCGTAACCGTTTTTGCTGCGGTATTCGTCCAGCCACACTGGCTGTTTGTCATCGCGCAGACGCCAGGTCAGCGGATGCGTTTCGGGAGTACGGATAATGTTTTTCATTTATACAGCTcc                                                                                                                                                 <  1:1332564/150‑1 (MQ=255)
       aCAGCCCGGTCAGCGCCTTACGCGCGCCTTCGTAACCGTTTTTGCTGCGGTATTCGTCCAGCCACACTGGCTGTTTGTCATCGCGCAGACGCCAGGTCAGCGGATGCGTTTCGGGAGTACGGATAATGTTTTTCATTTATACAGCTCcag                                                                                                                                               <  1:1818314/150‑1 (MQ=255)
        cAGCCCGGTCAGCGCCTTACGCGCGCCTTCGTAACCGTTTTTGCTGCGGTATTCGTCCAGCCACACTGGCTGTTTGTCATCGCGCAGACGCCAGGTCAGCGGATGCGTTTCGGGAGTACGGATAATGTTTTTCATTTATACCGCTCcagc                                                                                                                                              >  2:499869/1‑150 (MQ=255)
          gCCCGGTCAGCGCCTTACGCGCGCCTTCGTAACCGTTTTTGCTGCGGTATTCGTCCAGCCACACTGGCTGTTTGTCATCGCGCAGACGCCAGGTCAGCGGATGCGTTTCGGGAGTACGGATAATGTTTTTCATTTATACCGCTCcagcag                                                                                                                                            <  2:4258877/150‑1 (MQ=255)
            ccGGTCAGCGCCTTACGCGCGCCTTCGTAACCGTTTTTGCTGCGGTATTCGTCCAGCCACACTGGCTGTTTGTCATCGCGCAGACGCCAGGTCAGCGGATGCGTTTCGGGAGTACGGATAATGTTTTTCATTTATACAGCTCCAGCAGtt                                                                                                                                          <  1:3164006/150‑1 (MQ=255)
             cGGTCAGCGCCTTACGCGCGCCTTCGTAACCGTTTTTGCTGCGGTATTCGTCCAGCCACACTGGCTGTTTGTCATCGCGCAGACGCCAGGTCAGCGGATGCGTTTCGGGAGTACGGATAATGTTTTTCATTTATACAGCTCCAGCAGTTc                                                                                                                                         <  1:445673/150‑1 (MQ=255)
               gTCAGCGCCTTACGCGCGCCTTCGTAACCGTTTTTGCTGCGGTATTCGTCCAGCCACACTGGCTGTTTGTCATCGCGCAGACGCCAGGTCAGCGGATGCGTTTCGGGAGTACGGATAATGTTTTTCATTTATACCGCTCCAGCAGTTCAg                                                                                                                                       <  2:3141673/150‑1 (MQ=255)
                     gcCTTACGCGCGCCTTCGTAACCGTTTTTGCTGCGGTATTCGTCCAGCCACACTGGCTGTTTGTCATCGCGCAGACGCCAGGTCAGCGGATGCGTTTCGGGAGTACGGATAATGTTTTTCATTTATACCGCTCCAGCAGTTCAGGGATCg                                                                                                                                 >  2:2850268/1‑150 (MQ=255)
                       cTTACGCGCGCCTTCGTAACCGTTTTTGCTGCGGTATTCGTCCAGCCACACTGGCTGTTTGTCATCGCGCAGACGCCAGGTCAGCGGATGCGTTTCGGGAGTACGGATAATGTTTTTCATTTATACAGCTCCAGCAGTTCAGGGATCGCt                                                                                                                               >  2:1114810/1‑150 (MQ=255)
                       cTTACGCGCGCCTTCGTAACCGTTTTTGCTGCGGTATTCGTCCAGCCACACTGGCTGTTTGTCATCGCGCAGACGCCAGGTCAGCGGATGCGTTTCGGGAGTACGGATAATGTTTTTCATTTATACAGCTCCAGCAGTTCAGGGATCGCt                                                                                                                               <  1:3680714/150‑1 (MQ=255)
                       cTTACGCGCGCCTTCGTAACCGTTTTTGCTGCGGTATTCGTCCAGCCACACTGGCTGTTTGTCATCGCGCAGACGCCAGGTCAGCGGATGCGTTTCGGGAGTACGGATAATGTTTTTCATTTATACAGCTCCAGCAGTTCAGGGATCGCt                                                                                                                               <  1:3680153/150‑1 (MQ=255)
                          aCGCGCGCCTTCGTAACCGTTTTTGCTGCGGTATTCGTCCAGCCACACTGGCTGTTTGTCATCGCGCAGACGCCAGGTCAGCGGATGCGTTTCGGGAGTACGGATAATGTTTTTCATTTATACCGCTCCAGCAGTTCAGGGATCGCTTcc                                                                                                                            >  1:786/1‑150 (MQ=255)
                             cgcgcCTTCGTAACCGTTTTTGCTGCGGTATTCGTCCAGCCACACTGGCTGTTTGTCATCGCGCAGACGCCAGGTCAGCGGATGCGTTTCGGGAGTACGGATAATGTTTTTCATTTATACCGCTCCAGCAGTTCAGGGATCGCTTCCggg                                                                                                                         >  2:3596367/1‑150 (MQ=255)
                                 ccTTCGTAACCGTTTTTGCTGCGGTATTCGTCCAGCCACACTGGCTGTTTGTCATCGCGCAGACGCCAGGTCAGCGGATGCGTTTCGGGAGTACGGATAATGTTTTTCATTTATACAGCTCCAGCAGTTCAGGGATCGCTTCCGGGGTCa                                                                                                                     <  1:4267403/150‑1 (MQ=255)
                                  cTTCGTAACCGTTTTTGCTGCGGTATTCGTCCAGCCACACTGGCTGTTTGTCATCGCGCAGACGCCAGGTCAGCGGATGCGTTTCGGGAGTACGGATAATGTTTTTCATTTATACCGCTCCAGCAGTTCAGGGATCGCTTCCGGGGTCAg                                                                                                                    >  1:3305172/1‑150 (MQ=255)
                                  cTTCGTAACCGTTTTTGCTGCGGTATTCGTCCAGCCACACTGGCTGTTTGTCATCGCGCAGACGCCAGGTCAGCGGATGCGTTTCGGGAGTACGGATAATGTTTTTCATTTATACAGCTCCAGCAGTTCAGGGATCGCTTCCGGGGTCAg                                                                                                                    >  1:3111627/1‑150 (MQ=255)
                                  cTTCGTAACCGTTTTTGCTGCGGTATTCGTCCAGCCACACTGGCTGTTTGTCATCGCGCAGACGCCAGGTCAGCGGATGCGGTTCGGGATTACGGATAATGTTTTTCATTTATACAGCTACAGCAGTTCAGGGATCGCTTCCGGGGTCAg                                                                                                                    >  1:3437655/1‑150 (MQ=255)
                                      gTAACCGTTTTTGCTGCGGTATTCGTCCAGCCACACTGGCTGTTTGTCATCGCGCAGACGCCAGGTCAGCGGATGCGTTTCGGGAGTACGGATAATGTTTTTCATTTATACCGCTCCAGCAGTTCAGGGATCGCTTCCGGGGTCAGATgc                                                                                                                >  1:891722/1‑150 (MQ=255)
                                       tAACCGTTTTTGCTGCGGTATTCGTCCAGCCACACTGGCTGTTTGTCATCGCGCAGACGCCAGGTCAGCGGATGCGTTTCGGGAGTACGGATAATGTTTTTCATTTATACCGCTCCAGCAGTTCAGGGATCGCTTCCGGGGTCAGATgcg                                                                                                               <  2:1317189/150‑1 (MQ=255)
                                        aaCCGTTTTTGCTGCGGTATTCGTCCAGCCACACTGGCTGTTTGTCATCGCGCAGACGCCAGGTCAGCGGATGCGTTTCGGGAGTACGGATAATGTTTTTCATTTATACAGCTCCAGCAGTTCAGGGATCGCTTCCGGGGTCAGATgcgc                                                                                                              <  2:2480786/150‑1 (MQ=255)
                                         aCCGTTTTTGCTGCGGTATTCGTCCAGCCACACTGGCTGTTTGTCATCGCGCAGACGCCAGGTCAGCGGATGCGTTTCGGGAGTACGGATAATGTTTTTCATTTATACCGCTCCAGCAGTTCAGGGATCGCTTCCGGGGTCAGATGcgcg                                                                                                             >  1:3004829/1‑150 (MQ=255)
                                         aCCGTTTTTGCTGCGGTATTCGTCCAGCCACACTGGCTGTTTGTCATCGCGCAGACGCCAGGTCAGCGGATGCGTTTCGGGAGTACGGATAATGTTTTTCATTTATACCGCTCCAGCAGTTCAGGGATCGCTTCCGGGGTCAGATGcgcg                                                                                                             <  2:3640973/150‑1 (MQ=255)
                                              ttttGCTGCGGTATTCGTCCAGCCACACTGGCTGTTTGTCATCGCGCAGACGCCAGGTCAGCGGATGCGTTTCGGGAGTACGGATAATGTTTTTCATTTATACCGCTCCAGCAGTTCAGGGATCGCTTCCGGGGTCAGATGCGCGTGAgt                                                                                                        <  2:1458727/150‑1 (MQ=255)
                                                ttGCTGCGGTATTCGTCCAGCCACACTGGCTGTTTGTCATCGCGCAGACGCCAGGTCAGCGGATGCGTTTCGGGAGTACGGATAATGTTTTTCATTTATACCGCTCCAGCAGTTCAGGGATCGCTTCCGGGGTCAGATGCGCGTGAgtgt                                                                                                      <  2:3687984/150‑1 (MQ=255)
                                                 tgctgcGGTATTCGTCCAGCCACACTGGCTGTTTGTCATCGCGCAGACGCCAGGTCAGCGGATGCGTTTCGGGAGTACGGATAATGTTTTTCATTTATACCGCTCCAGCAGTTCAGGGATCGCTTCCGGGGTCAGATGCGCGTGAGTGTc                                                                                                     >  2:2144605/1‑150 (MQ=255)
                                                   ctgcGGTATTCGTCCAGCCACACTGGCTGTTTGTCATCGCGCAGACGCCAGGTCAGCGGATGCGTTTCGGGAGTACGGATAATGTTTTTCATTTATACCGCTCCAGCAGTTCAGGGATCGCTTCCGGGGTCAGATGCGCGTGAGTGTCCt                                                                                                   <  1:4002354/150‑1 (MQ=255)
                                                    tgcGGTATTCGTCCAGCCACACTGGCTGTTTGTCATCGCGCAGACGCCAGGTCAGCGGATGCGTTTCGGGAGTACGGATAATGTTTTTCATTTATACCGCTCCAGCAGTTCAGGGATCGCTTCCGGGGTCAGATGCGCGTGAGTGTCCTc                                                                                                  <  1:3868349/150‑1 (MQ=255)
                                                    tgcGGTATTCGTCCAGCCACACTGGCTGTTTGTCATCGCGCAGACGCCAGGTCAGCGGATGCGTTTCGGGAGTACGGATAATGTTTTTCATTTATACCGCTCCAGCAGTTCAGGGATCGCTTCCGGGGTCAGATGCGCGTGAGTGTCCTc                                                                                                  >  2:1484597/1‑150 (MQ=255)
                                                    tgcGGTATTCGTCCAGCCACACTGGCTGTTTGTCATCGCGCAGACGCCAGGTCAGCGGATGCGTTTCGGGAGTACGGATAATGTTTTTCAGTTATACCGCTCCAGCAGTTCAGGGATCGCTTCCGGGGTCAGATGCGCGTGAGTGTCCTc                                                                                                  >  2:3859378/1‑150 (MQ=255)
                                                      cGGTATTCGTCCAGCCACACTGGCTGTTTGTCATCGCGCAGACGCCAGGTCAGCGGATGCGTTTCGGGAGTACGGATAATGTTTTTCATTTATACCGCTCCAGCAGTTCAGGGATCGCTTCCGGGGTCAGATGCGCGTGAGTGTCCTCAt                                                                                                >  1:2666978/1‑150 (MQ=255)
                                                      cGGTATTCGTCCAGCCACACTGGCTGTTTGTCATCGCGCAGACGCCAGGTCAGCGGATGCGTTTCGGGAGTACGGATAATGTTTTTCATTTATACCGCTCCAGCAGTTCAGGGATCGCTTCCGGGGTCAGATGCGCGTGAGTGTCCTCAt                                                                                                <  1:4143955/150‑1 (MQ=255)
                                                       ggTATTCGTCCAGCCACACTGGCTGTTTGTCATCGCGCAGACGCCAGGTCAGCGGATGCGTTTCGGGAGTACGGATAATGTTTTTCATTTATACCGCTCCAGCAGTTCAGGGATCGCTTCCGGGGTCAGATGCGCGTGAGTGTCCTCATc                                                                                               >  1:1880115/1‑150 (MQ=255)
                                                       ggTATTCGTCCAGCCACACTGGCTGTTTGTCATCGCGCAGACGCCAGGTCAGCGGATGCGTTTCGGGAGTACGGATAATGTTTTTCATTTATACCGCTCCAGCAGTTCAGGGATCGCTTCCGGGGTCAGATGCGCGTGAGTGTCCTCATc                                                                                               <  2:1879805/150‑1 (MQ=255)
                                                        gTATTCGTCCAGCCACACTGGCTGTTTGTCATCGCGCAGACGCCAGGTCAGCGGATGCGTTTCGGGAGTACGGATAATGTTTTTCATTTATACCGCTCCAGCAGTTCAGGGATCGCTTCCGGGGTCAGATGCGCGTGAGTGTCCTCATCg                                                                                              >  2:2742342/1‑150 (MQ=255)
                                                            tCGTCCAGCCACACTGGCTGTTTGTCATCGCGCAGACGCCAGGTCAGCGGATGCGTTTCGGGAGTACGGATAATGTTTTTCATTTATACCGCTCCAGCAGTTCAGGGATCGCTTCCGGGGTCAGATGCGCGTGAGTGTCCTCATCGatca                                                                                          <  1:2766027/150‑1 (MQ=255)
                                                            tCGTCCAGCCACACTGGCTGTTTGTCATCGCGCAGACGCCAGGTCAGCGGATGCGTTTCGGGAGTACGGATAATGTTTTTCATTTATACCGCTCCAGCAGTTCAGGGATCGCTTCCGGGGTCAGATGCGCGTGAGTGTCCTCATCGatca                                                                                          <  1:503324/150‑1 (MQ=255)
                                                            gcGTCCAGCCACACTGGCTGTTTGTCATCGCGCAGACGCCAGGTCAGCGGATGCGTTTCGGGAGTACGGATAATGTTTTTCATTTATACAGCTCCAGCAGTTCAGGGATCGCTTCCGGGGTCAGATGCGCGTGAGTGTCCTCATCGatca                                                                                          <  2:2558938/149‑1 (MQ=255)
                                                               tCCGGCCACACTGGCTGTTTGTCATCGCGCAGACGCCAGGTCAGCGGATGCGTTTCGGGAGTACGGATAATGTTTTTCATTTATACCGCTCCAGCAGTTCAGGGATCGCTTCCGGGGTCAGATGCGCGTGAGTGTCCTCATCGAtcatca                                                                                       <  2:1267058/150‑1 (MQ=255)
                                                               tCCAGCCACACTGGCTGTTTGTCATCGCGCAGACGCCAGGTCAGCGGATGCGTTTCGGGAGTACGGATAATGTTTTTCATTTATACCGCTCCAGCAGTTCAGGGATCGCTTCCGGGGTCAGATGCGCGTGAGTGTCCTCATCGAtcatca                                                                                       <  2:3551220/150‑1 (MQ=255)
                                                                ccAGCCACACTGGCTGTTTATCATCGCGCAGACGCCAGGTCAGCGGATGCGTTTCGGGAGTACGGATAATGTTTTTCATTTATACCGCTCCAGCAGTTCAGGGATCGCTTCCGGGGTCAGATGCGCGTGAGTGTCCTCATCGATcatcat                                                                                      >  1:2028117/1‑150 (MQ=255)
                                                                   gCCACACTGGCTGTTTGTCATCGCGCAGACGCCAGGTCAGCGGATGCGTTTCGGGAGTACGGATAATGTTTTTCATTTATACCGCTCCAGCAGTTCAGGGATCGCTTCCGGGGTCAGATGCGCGTGAGTGTCCTCATCGATCATCATGtt                                                                                   <  1:108547/150‑1 (MQ=255)
                                                                    ccACACTGGCTGTTTGTCATCGCGCAGACGCCAGGTCAGCGGATGCGTTTCGGGAGTACGGATAATGTTTTTCATTTATACCGCTCCAGCAGTTCAGGGATCGCTTCCGGGGTCAGATGCGCGTGAGTGTCCTCATCGATCATCATGttt                                                                                  <  1:352571/150‑1 (MQ=255)
                                                                     cacaCTGGCTGTTTGTCATCGCGCAGACGCCAGGTCAGCGGATGCGTTTCGGGAGTACGGATAATGTTTTTCATTTATACCGCTCCAGCAGTTCAGGGATCGCTTCCGGGGTCAGATGCGCGTGAGTGTCCTCATCGATCATCATGTTTg                                                                                 >  2:1303693/1‑150 (MQ=255)
                                                                     cacaCTGGCTGTTTGTCATCGCGCAGACGCCAGGTCAGCGGATGCGTTTCGGGAGTACGGATAATGTTTTTCATTTATACAGCTCCAGCAGTTCAGGGATCGCTTCCGGGGTCAGATGCGCGTGAGTGTCCTCATCGATCATCATGTTTg                                                                                 <  1:2314388/150‑1 (MQ=255)
                                                                        acTGGCTGTTTGTCATCGCGCAGACGCCAGGTCAGCGGATGCGTTTCGGGAGTACGGATAATGTTTTTCATTTATACCGCTCCAGCAGTTCAGGGATCGCTTCCGGGGTCAGATGCGCGTGAGTGTCCTCATCGATCATCATGTTTGGcc                                                                              >  1:1040504/1‑150 (MQ=255)
                                                                         cTGGCTGTTTGTCATCGCGCAGACGCCAGGTCAGCGGATGCGTTTCGGGAGTACGGATAATGTTTTTCATTTATACCGCTCCAGCAGTTCAGGGATCGCTTCCGGGGTCAGATGCGCGTGAGTGTCCTCATCGATCATCATGTTTGGccc                                                                             <  2:428285/150‑1 (MQ=255)
                                                                           ggCTGTTTGTCATCGCGCAGACGCCAGGTCAGCGGATGCGTTTCGGGAGTACGGATAATGTTTTTCATTTATACCGCTCCAGCAGTTCAGGGATCGCTTCCGGGGTCAGATGCGCGTGAGTGTCCTCATCGATCATCATGTTTGGCCCtt                                                                           <  2:3974578/150‑1 (MQ=255)
                                                                            gCTGTTTGTCATCGCGCAGACGCCAGGTCAGCGGATGCGTTTCGGGAGTACGGATAATGTTTTTCATTTATACCGCTCCAGCAGTTCAGGGATCGCTTCCGGGGTCAGATGCGCGTGAGTGTCCTCATCGATCATCATGTTTGGCCCttt                                                                          <  2:4483571/150‑1 (MQ=255)
                                                                            gCTGTTTGTCATCGCGCAGACGCCAGGTCAGCGGATGCGTTTCGGGAGTACGGATAATGTTTTTCATTTATACCGCTCCAGCAGTTCAGGGATCGCTTCCGGGGTCAGATGCGCGTGAGTGTCCTCATCGATCATCATGTTTGGCCCttt                                                                          <  2:3113923/150‑1 (MQ=255)
                                                                            gCTGTTTGTCATCGCGCAGACGCCAGGTCAGCGGATGCGTTTCGGGAGTACGGATAATGTTTTTCATTTATACCGCTCCAGCAGTTCAGGGATCGCTTCCGGGGTCAGATGCGCGTGAGTGTCCTCATCGATCATCATGTTTGGCCCttt                                                                          <  2:2237998/150‑1 (MQ=255)
                                                                              tGTTTGTCATCGCGCAGACGCCAGGTCAGCGGATGCGTTTCGGGAGTACGGATAATGTTTTTCATCTATACCGCTCCAGCAGTTCAGGGATCGCTTCCGGGGTCAGATGCGCGTGAGTGTCCTCATCGATCATCATGTTTGGCCCTTTAt                                                                        >  2:2473894/1‑150 (MQ=255)
                                                                                tttGTCATCGCGCAGACGCCAGGTCAGCGGATGCGTTTCGGGAGTACGGATAATGTTTTTCATTTATACCGCTCCAGCAGTTCAGGGATCGCTTCCGGGGTCAGATGCGCGTGAGTGTCCTCATCGATCATCATGTTTGGCCCTTTATca                                                                      <  1:2128383/150‑1 (MQ=255)
                                                                                  tGTCATCGCGCAGACGCCAGGTCAGCGGATGCGTTTCGGGAGTACGGATAATGTTTTTCATTTATACAGCTCCAGCAGTTCAGGGATCGCTTCCGGGGTCAGATGCGCGTGAGTGTCCTCATCGATCATCATGTTTGGCCCTTTATcaca                                                                    >  2:3036693/1‑150 (MQ=255)
                                                                                     cATCGCGCAGACGCCAGGTCAGCGGATGCGTTTCGGGAGTACGGATAATGTTTTTCATTTATACAGCTCCAGCAGTTCAGGGATCGCTTCCGGGGTCAGATGCGCGTGAGTGTCCTCATCGATCATCATGTTTGGCCCTTTATCACAGtt                                                                 <  2:2562005/150‑1 (MQ=255)
                                                                                      aTCGCGCAGACGCCAGGTCAGCGGATGCGTTTCGGGAGTACGGATAATGTTTTTCATTTATACCGCTCCAGCAGTTCAGGGATCGCTTCCGGGGTCAGATGCGCGTGAGTGTCCTCATCGATCATCATGTTTGGCCCTTTATCACAGTTc                                                                >  1:243552/1‑150 (MQ=255)
                                                                                       tCGCGCAGACGCCAGGTCAGCGGATGCGTTTCGGGAGTACGGATAATGTTTTTCATTTATACCGCTCCAGCAGTTCAGGGATCGCTTCCGGGGTCAGATGCGCGTGAGTGTCCTCATCGATCATCATGTTTGGCCCTTTATCACAGTTcc                                                               <  2:902545/150‑1 (MQ=255)
                                                                                         gcgcAGACGCCAGGTCAGCGGATGCGTTTCGGGAGTACGGATAATGTTTTTCATTTATACAGCTCCAGCAGTTCAGGGATCGCTTCCGGGGTCAGATGCGCGTGAGTGTCCTCATCGATCATCTTGTTTGGCCCTTTATCACAGTTcccc                                                             <  1:4071163/150‑1 (MQ=255)
                                                                                           gcAGACGCCAGGTCAGCGGATGCGTTTCGGGAGTACGGATAATGTTTTTCATTTATACCGCTCCAGCAGTTCAGGGATCGCTTCCGGGGTCAGATGCGCGTGAGTGTCCTCATCGATCATCATGTTTGGCCCTTTATCACAGTTCCCCAg                                                           <  2:4532594/150‑1 (MQ=255)
                                                                                                cGCCAGGTCAGCGGATGCGTTTCGGGAGTACGGATAATGTTTTTCATTTATACCGCTCCAGCAGTTCAGGGATCGCTTCCGGGGTCAGATGCGCGTGAGTGTCCTCATCGATCATCATGTTTGGCCCTTTATCACAGTTCCCCAGgcagc                                                      >  2:1961202/1‑150 (MQ=255)
                                                                                                 gCCAGGTCAGCGGATGCGTTTCGGGAGTACGGATAATGTTTTTCATTTATACCGCTCCAGCAGTTCAGGGATCGCTTCCGGGGTCAGATGCGCGTGAGTGTCCTCATCGATCATCATGTTTGGCCCTTTATCACAGTTCCCCAGgcagca                                                     >  2:1002809/1‑150 (MQ=255)
                                                                                                     ggTCAGCGGATGCGTTTCGGGAGTACGGATAATGTTTTTCATTTATACCGCTCCAGCAGTTCAGGGATCGCTTCCGGGGTCAGATGCGCGTGAGTGTCCTCATCGATCATCATGTTTGGCCCTTTATCACAGTTCCCCAGGCAGCAAGtt                                                 >  1:2532332/1‑150 (MQ=255)
                                                                                                      gTCAGCGGATGCGTTTCGGGAGTACGGATAATGTTTTTCATTTATACCGCTCCAGCAGTTCAGGGATCGCTTCCGGGGTCAGATGCGCGTGAGTGTCCTCATCGATCATCATGTTTGGCCCTTTATCACAGTTCCCCAGGCAGCAAGTTg                                                <  2:1828059/150‑1 (MQ=255)
                                                                                                         aGCGGATGCGTTTCGGGAGTACGGATAATGTTTTTCATTTATACCGCTCCAGCAGTTCAGGGATCGCTTCCGGGGTCAGATGCGCGTGAGTGTCCTCATCGATCATCATGTTTGGCCCTTTATCACAGTTCCCCAGGCAGCAAGTTGgca                                             >  2:4126683/1‑150 (MQ=255)
                                                                                                         aGCGGATGCGTTTCGGGAGTACGGATAATGTTTTTCATTTATACCGCTCCAGCAGTTCAGGGATCGCTTCCGGGGTCAGATGCGCGTGAGTGTCCTCATCGATCATCATGTTTGGCCCTTTATCACAGTTCCCCAGGCAGCAAGTTGgca                                             >  1:2154768/1‑150 (MQ=255)
                                                                                                             gATGCGTTTCGGGAGTACGGATAATGTTTTTCATTTATACCGCTCCAGCAGTTCAGGGATCGCTTCCGGGGTCAGATGCGCGTGAGTGTCCTCATCGATCATCATGTTTGGCCCTTTATCACAGTTCCCCAGGCAGCAAGTTGGcagcag                                         <  1:1725813/150‑1 (MQ=255)
                                                                                                             gATGCGTTTCGGGAGTACGGATAATGTTTTTCATTTATACAGCTCCAGCAGTTCAGGGATCGCTTCCGGGGTCAGATGCGCGTGAGTGTCCTCATCGATCATCATGTTTGGCCCTTTATCACAGTTCCCCAGGCAGCAAGTTGGcagcag                                         <  2:3296488/150‑1 (MQ=255)
                                                                                                              aTGCGTTTCGGGAGTACGGATAATGTTTTTCATTTATACAGCTCCAGCAGTTCAGGGATCGCTTCCGGGGTCAGATGCGCGTGAGTGTCCTCATCGATCATCATGTTTGGCCCTTTATCACAGTTCCCCAGGCAGCAAGTTGGCagcagc                                        >  2:3218169/1‑150 (MQ=255)
                                                                                                                 cGTTTCGGGAGTACGGATAATGTTTTTCATTTATACCGCTCCAGCAGTTCAGGGATCGCTTCCTTGGTCAGATGCGCGTGAGTTTCCTCATCTATCATCATGTTTGGCCCTTTATCTCTTTTCCCCAGGCAGCAAGTTGGCAGCAGCGTa                                     <  1:309/150‑1 (MQ=255)
                                                                                                                 cGTTTCGGGAGTACGGATAATGTTTTTCATTTATACCGCTCCAGCAGTTCAGGGATCGCTTCCGGGGTCAGATGCGCGTGAGTGTCCTCATCGATCATCATGTTTGGCCCTTTATCACAGTTCCCCAGGCAGCAAGTTGGCAGCAGCGTa                                     >  1:1261680/1‑150 (MQ=255)
                                                                                                                 cGTTTCGGGAGTACGGATAATGTTTTTCATTTATACCGCTCCAGCAGTTCAGGGATCGCTTCCGGGGTCAGATGCGCGTGAGTGTCCTCATCGATCATCATGTTTGGCCCTTTATCACAGTTCCCCAGGCAGCAAGTTGGCAGCAGCGTa                                     <  2:3418004/150‑1 (MQ=255)
                                                                                                                      cGGGAGTACGGATAATGTTTTTCATTTATACCGCTCCAGCAGTTCAGGGATCGCTTCCGGGGTCAGATGCGCGTGAGTGTCCTCATCGATCATCATGTTTGGCCCTTTATCACAGTTCCCCAGGCAGCAAGTTGGCAGCAGCGTAAAGCg                                >  1:4388312/1‑150 (MQ=255)
                                                                                                                       gggAGTACGGATACTGTTTTTCATTTATACCGCTCCAGCAGTTCAGGGCTCGCTTCCGGGGTCAGATGCGCGTGAGTGTCCTCATCGATCATCATGTTTGGCCCTTTATCACAGTTCCCCAGGCAGCAAGTTGGCAGCAGCGTAAAGCgg                               <  1:2590319/150‑1 (MQ=255)
                                                                                                                       gggAGTACGGATAATGTTTTTCATTTATACCGCTCCAGCAGTTCAGGGATCGCTTCCGGGGTCAGATGCGCGTGAGTGTCCTCATCGATCATCATGTTTGGCCCTTTATCACAGTTCCCCAGGCAGCAAGTTGGCAGCAGCGTAAAGCgg                               <  2:3458587/150‑1 (MQ=255)
                                                                                                                       gggAGTACGGATAATGTTTTTCATTTATACCGCTCCAGCAGTTCAGGGATCGCTTCCGGGGTCAGATGCGCGTGAGTGTCCTCATCGATCATCATGTTTGGCCCTTTATCACAGTTCCCCAGGCAGCAAGTTGGCAGCAGCGTAAAGCgg                               <  1:2972817/150‑1 (MQ=255)
                                                                                                                       gggAGTACGGATAATGTTTTTCATTTATACCGCTCCAGCAGTTCAGGGATCGCGTCCGGGGTCAGATGCGCGTGAGTGTCCTCATCGATCATCATGTTTGGCCCTTTATCACAGTTCCCCAGGCAGCAAGTTGGCAGCAGCGTAAAGCgg                               <  2:818209/150‑1 (MQ=255)
                                                                                                                       gggAGTACGGATAATGTTTTTCATTTATACAGCTCCAGCAGTTCAGGGATCGCTTCCGGGGTCAGATGCGCGTGAGTGTCCTCATCGATCATCATGTTTGGCCCTTTATCACAGTTCCCCAGGCAGCAAGTTGGCAGCAGCGTAAAGCgg                               <  1:692832/150‑1 (MQ=255)
                                                                                                                        ggAGTACGGATAATGTTTTTCATTTATACCGCTCCAGCAGTTCAGGGATCGCTTCCGGGGTCAGATGCGCGTGAGTGTCCTCATCGATCATCATGTTTGGCCCTTTATCACAGTTCCCCAGGCAGCAAGTTGGCAGCAGCGTAAAGCGGc                              <  1:1571544/150‑1 (MQ=255)
                                                                                                                               ggATAATGTTTTTCATTTATACCGCTCCAGCAGTTCAGGGATCGCTTCCGGGGTCAGATGCGCGTGAGTGTCCTCATCGATCATCATGTTTGGCCCTTTATCACAGTTCCCCAGGCAGCAAGTTGGCAGCAGCGTAAAGCGGCCATCaaa                       >  2:14202/1‑150 (MQ=255)
                                                                                                                                   aaTGTTTTTCATTTATACAGCTCCAGCAGTTCAGGGATCGCTTCCGGGGTCAGATGCGCGTGAGTGTCCTCATCGATCATCATGTTTGGCCCTATATCACAGTTCCCCAGGCAGCAAGTTGGCAGCAGCGTAAAGCGGCCATCAAATGTc                   >  1:941537/1‑150 (MQ=255)
                                                                                                                                    aTGTTTTTCATTTATACCGCTCCAGCAGTTCAGGGATCGCTTCCGGGGTCAGATGCGCGTGAGTGTCCTCATCGATCATCATGTTTGGCCCTTTATCACAGTTCCCCAGGCAGCAAGTTGGCAGCAGCGTAAAGCGGCCATCAAATGTCg                  <  2:206232/150‑1 (MQ=255)
                                                                                                                                    aTGTTTTTCATTTATACCGCTCCAGCAGTTCAGGGATCGCTTCCGGGGTCAGATGCGCGTGAGTGTCCTCATCGATCATCATGTTTGGCCCTTTATCACAGTTCCCCAGGCAGCAAGTTGGCAGCAGCGTAAAGCGGCCATCAAATGTCg                  >  2:2336355/1‑150 (MQ=255)
                                                                                                                                     tGTTTTTCATTTATACCGCTCCAGCAGTTCAGGGATCGCTTCCGGGGTCAGATGCGCGTGAGTGTCCTCATCGATCATCATGTTTGGCCCTTTATCACAGTTCCCCAGGCAGCAAGTTGGCAGCAGCGTAAAGCGGCCATCAAATGTCGt                 >  2:169713/1‑150 (MQ=255)
                                                                                                                                      gTTTTTCATTTATACCGCTCCAGCAGTTCAGGGATCGCTTCCGGGGTCAGATGCGCGTGAGTGTCCTCATCGATCATCATGTTTGGCCCTTTATCACAGTTCCCCAGGCAGCAAGTTGGCAGCAGCGTAAAGCGGCCATCAAATGTCGtt                <  1:2096128/150‑1 (MQ=255)
                                                                                                                                         tttCATTTATACCGCTCCAGCAGTTCAGGGATCGCTTCCGGGGTCAGATGCGCGTGAGTGTCCTCATCGATCATCATGTTTGGCCCTTTATCACAGTTCCCCAGGCAGCAAGTTGGCAGCAGCGTAAAGCGGCCATCAAATGTCGTTTGc             >  1:4314960/1‑150 (MQ=255)
                                                                                                                                          ttCATTTATACCGCTCCAGCAGTTCAGGGATCGCTTCCGGGGTCAGATGCGCGTGAGTGTCCTCATCGATCATCATGTTTGGCCCTTTATCACAGTTCCCCAGGCAGCAAGTTGGCAGCAGCGTAAAGCGGCCATCAAATGTCGTTTGcc            >  1:376436/1‑150 (MQ=255)
                                                                                                                                           tCATTTATACCGCTCCAGCAGTTCAGGGATCGCTTCCGGGGTCAGATGCGCGTGAGTGTCCTCATCGATCATCATGTTTGGCCCTTTATCACAGTTCCCCAGGCAGCAAGTTGGCAGCAGCGTAAAGCGGCCATCAAATGTCGTTTGccc           <  1:3308941/150‑1 (MQ=255)
                                                                                                                                               ttATACCGCTCCAGCAGTTCAGGGATCGCTTCCGGGGTCAGATGCGCGTGAGTGTCCTCATCGATCATCATGTTTGGCCCTTTATCACAGTTCCCCAGGCAGCAAGTTGGCAGCAGCGTAAAGCGGCCATCAAATGTCGTTTGCCCTGGt       <  2:787162/150‑1 (MQ=255)
                                                                                                                                                 ataCCGCTCCAGCAGTTCAGGGATCGCTTCCGGGGTCAGATGCGCGTGAGTGTCCTCATCGATCATCATGTTTGGCCCTTTATCACAGTTCCCCAGGCAGCAAGTTGGCAGCAGCGTAAAGCGGCCATCAAATGTCGTTTGCCCTGGttt     >  1:144242/1‑150 (MQ=255)
                                                                                                                                                 ataCCGCTCCAGCAGTTCAGGGATCGCTTCCGGGGTCAGATGCGCGTGAGTGTCCTCATCGATCATCATGTTTGGCCCTTTATCACAGTTCCCCAGGCAGCAAGTTGGCAGCAGCGTAAAGCGGCCATCAAATGTCGTTTGCCCTGGttt     >  2:4054278/1‑150 (MQ=255)
                                                                                                                                                 ataCCGCTCCAGCAGTTCAGGGATCGCTTCCGGGGTCAGATGCGCGTGAGTGTCCTCATCGATCATCATGTTTGGCCCTTTATCACAGTTCCCCAGGCAGCAAGTTGGCAGCAGCGTAAAGCGGCCATCAAATGTCGTTTGCCCTGGttt     >  1:731381/1‑150 (MQ=255)
                                                                                                                                                   aCCGCTCCAGCAGTTCAGGGATCGCTTCCGGGGTCAGATGCGCGTGAGTGTCCTCATCGATCATCATGTTTGGCCCTTTATCACAGTTCCCCAGGCAGCAAGTTGGCAGCAGCGTAAAGCGGCCATCAAATGTCGTTTGCCCTGGTTTGa   <  1:2010161/150‑1 (MQ=255)
                                                                                                                                                    ccGCTCCAGCAGTTCAGGGATCGCTTCCGGGGTCAGATGCGCGTGAGTGTCCTCATCGATCATCATGTTTGGCCCTTTATCACAGTTCCCCAGGCAGCAAGTTGGCAGCAGCGTAAAGCGGCCATCAAATGTCGTTTGCCCTGGTTTGAt  <  2:4514323/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
TCCGGAGACAGCCCGGTCAGCGCCTTACGCGCGCCTTCGTAACCGTTTTTGCTGCGGTATTCGTCCAGCCACACTGGCTGTTTGTCATCGCGCAGACGCCAGGTCAGCGGATGCGTTTCGGGAGTACGGATAATGTTTTTCATTTATACCGCTCCAGCAGTTCAGGGATCGCTTCCGGGGTCAGATGCGCGTGAGTGTCCTCATCGATCATCATGTTTGGCCCTTTATCACAGTTCCCCAGGCAGCAAGTTGGCAGCAGCGTAAAGCGGCCATCAAATGTCGTTTGCCCTGGTTTGAT  >  CP009273/2394892‑2395189

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A