Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F121 I0 R1 856 273.6 9081716 94.1% 8545894 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,573,363 C→A 14.6% V123V (GTC→GTA)  tktB → transketolase 2, thiamine triphosphate‑binding

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,573,3630CA14.6% 884.7 / 98.7 274V123V (GTC→GTA) tktBtransketolase 2, thiamine triphosphate‑binding
Reads supporting (aligned to +/- strand):  ref base C (117/117);  new base A (28/12);  total (145/129)
Fisher's exact test for biased strand distribution p-value = 2.52e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 280 total aligned reads displayed.

CTACATCTGACCGGTTACGACCTGCCGCTGGAAGAACTGAAGAACTTCCGTCAGTTGCATTCGAAAACCCCAGGCCACCCGGAGATTGGCTATACGCCAGGCGTTGAAACCACCACCGGCCCGCTTGGACAAGGTTTGGCGAACGCCGTCGGGCTGGCGATAGCAGAGCGTACACTGGCGGCGCAGTTTAACCAGCCAGACCATGAGATCGTCGATCACTTCACCTATGTGTTTATGGGCGACGGCTGCCTGATGGAAGGTATTTCCCACGAAGTCTGTTCGCTGGCAGGCACGCTGGG  >  CP009273/2573214‑2573512
                                                                                                                                                     |                                                                                                                                                     
cTACATCTGACCGGTTACGACCTGCCGCTGGAAGAACTGAAGAACTTCCGTCAGTTGCATTCGAAAACCCCAGGCCACCCGGAGATTGGCTATACGCCAGGCGTTGAAACCACCACCGGCCCGCTTGGACAAGGTTTGGCGAACGCCGTc                                                                                                                                                       >  1:2744249/1‑150 (MQ=255)
cTACATCTGACCGGTTACGACCTGCCGCTGGAAGAACTGAAGAACTTCCGTCAGTTGCATTCGAAAACCCCAGGCCACCCGGAGATTGGCTATACGCCAGGCGTTGAAACCACCACCGGCCCGCTTGGACAAGGTTTGGCGAACGCCGTc                                                                                                                                                       >  1:786356/1‑150 (MQ=255)
  aCATCTGACCGGTTACGACCTGCCGCTGGAAGAACTGAAGAACTTCCGTCAGTTGCATTCGAAAACCCCAGGCCACCCGGAGATTGGCTATACGCCAGGCGTTGAAACCACCACCGGCCCGCTTGGACAAGGTTTGGCGAACGCCGTCgg                                                                                                                                                     <  1:752638/150‑1 (MQ=255)
      cTGACCGGTTACGACCTGCCGCTGGAAGAACTGAAGAACTTCCGTCAGTTGCATTCGAAAACCCCAGGCCACCCGGAGATTGGCTATACGCCAGGCGTTGAAACCACCACCGGCCCGCTTGGACAAGGTTTGGCGAACGCCGTCGGGCTg                                                                                                                                                 <  2:2329392/150‑1 (MQ=255)
       tGACCGGTTACGACCTGCCGCTGGAAGAACTGAAGAACTTCCGTCAGTTGCATTCGAAAACCCCAGGCCACCCGGAGATTGGCTATACGCCAGGCGTTGAAACCACCACCGGCCCGCTTGGACAAGGTTTGGCGAACGCCGTCGGGCTgg                                                                                                                                                >  2:4539254/1‑150 (MQ=255)
         aCCGGTTACGACCTGCCGCTGGAAGAACTGAAGAACTTCCGTCAGTTGCATTCGAAAACCCCAGGCCACCCGGAGATTGGCTATACGCCAGGCGTTGAAACCACCACCGGCCCGCTTGGACAAGGTTTGGCGAACGCCGTCGGGCTGGCg                                                                                                                                              <  1:4041480/150‑1 (MQ=255)
            ggTTACGACCTGCCGCTGGAAGAACTGAAGAACTTCCGTCAGTTGCATTCGAAAACCCCAGGCCACCCGGAGATTGGCTATACGCCAGGCGTTGAAACCACCACCGGCCCGCTTGGACAAGGTTTGGCGAACGCCGTCGGGCTGGCGATa                                                                                                                                           <  2:1322509/150‑1 (MQ=255)
            ggTTACGACCTGCCGCTGGAAGAACTGAAGAACTTCCGTCAGTTGCATTCGAAAACCCCAGGCCACCCGGAGATTGGCTATACGCCAGGCGTTGAAACCACCACCGGCCCGCTTGGACAAGGTTTGGCGAACGCCGTCGGGCTGGCGATa                                                                                                                                           <  1:3475558/150‑1 (MQ=255)
            ggTTACGACCTGCCGCTGGAAGAACTGAAGAACTTCCGTCAGTTGCATTCGAAAACCCCAGGCCACCCGGAGATTGGCTATACGCCAGGCGTTGAAACCACCACCGGCCCGCTTGGACAAGGTTTGGCGAACGCCGTCGGGCTGGCGATa                                                                                                                                           >  1:4537072/1‑150 (MQ=255)
             gTTACGACCTGCCGCTGGAAGAACTGAAGAACTTCCGTCAGTTGCATTCGAAAACCCCAGGCCACCCGGAGATTGGCTATACGCCAGGCGTTGAAACCACCACCGGCCCGCTTGGACAAGGTTTGGCGAACGCCGTCGGGCTGGCGATAg                                                                                                                                          <  2:3352170/150‑1 (MQ=255)
             gTTACGACCTGCCGCTGGAAGAACTGAAGAACTTCCGTCAGTTGCATTCGAAAACCCCAGGCCACCCGGAGATTGGCTATACGCCAGGCGTTGAAACCACCACCGGCCCGCTTGGACAAGGTTTGGCGAACGCCGTCGGGCTGGCGATAg                                                                                                                                          >  1:2937554/1‑150 (MQ=255)
             gTTACGACCTGCCGCTGGAAGAACTGAAGAACTTCCGTCAGTTGCATTCGAAAACCCCAGGCCACCCGGAGATTGGCTATACGCCAGGCGTTGAAACCACCACCGGCCCGCTTGGACAAGGTTTGGCGAACGCCGTCGGGCTGGCGATAg                                                                                                                                          >  1:4083541/1‑150 (MQ=255)
             gTTACGACCTGCCGCTGGAAGAACTGAAGAACTTCCGTCAGTTGCATTCGAAAACCCCAGGCCACCCGGAGATTGGCTATACGCCAGGCGTTGAAACCACCACCGGCCCGCTTGGACAAGGTTTGGCGAACGCCGTCGGGCTGGCGATAg                                                                                                                                          <  2:1231431/150‑1 (MQ=255)
               tACGACCTGCCGCTGGAAGAACTGAAGAACTTCCGTCAGTTGCATTCGAAAACCCCAGGCCACCCGGAGATTGGCTATACGCCAGGCGTTGAAACCACCACCGGCCCGCTTGGACAAGGTTTGGCGAACGCCGTCGGGCTGGCGATAGCa                                                                                                                                        >  1:2830194/1‑150 (MQ=255)
               tACGACCTGCCGCTGGAAGAACTGAAGAACTTCCGTCAGTTGCATTCGAAAACCCCAGGCCACCCGGAGATTGGCTATACGCCAGGCGTTGAAACCACCACCGGCCCGCTTGGACAAGGTTTGGCGAACGCCGTAGGGCTGGCGATAGCa                                                                                                                                        >  2:4492030/1‑150 (MQ=255)
                   aCCTGCCGCTGGAAGAACTGAAGAACTTCCGTCAGTTGCATTCGAAAACCCCAGGCCACCCGGAGATTGGCTATACGCCAGGCGTTGAAACCACCACCGGCCCGCTTGGACAAGGTTTGGCGAACGCCGTAGGGCTGGCGATAGCAGAGc                                                                                                                                    <  1:1776342/150‑1 (MQ=255)
                           cTGGAAGAACTGAAGAACTTCCGTCAGTTGCATTCGAAAACCCCAGGCCACCCGGAGATTGGCTATACGCCAGGCGTTGAAACCACCACCGGCCCGCTTGGACAAGGTTTGGCGAACGCCGTCGGGCTGGCGATAGCAGAGCGTACACTg                                                                                                                            <  1:1403756/150‑1 (MQ=255)
                            tGGAAGAACTGAAGAACTTCCGTCAGTTGCATTCGAAAACCCCAGGCCACCCGGAGATTGGCTATACGCCAGGCGTTGAAACCACCACCGGCCCGCTTGGACAAGGTTTGGCGAACGCCGTCGGGCTGGCGATAGCAGAGCGTACACTgg                                                                                                                           >  2:119161/1‑150 (MQ=255)
                             ggAAGAACTGAAGAACTTCCGTCAGTTGCATTCGAAAACCCCAGGCCACCCGGAGATTGGCTATACGCCAGGCGTTGAAACCACCACCGGCCCGCTTGGACAAGGTTTGGCGAACGCCGTCGGGCTGGCGATAGCAGAGCGTACACTggc                                                                                                                          <  2:1143912/150‑1 (MQ=255)
                             ggAAGAACTGAAGAACTTCCGTCAGTTGCATTCGAAAACCCCAGGCCACCCGGAGATTGGCTATACGCCAGGCGTTGAAACCACCACCGGCCCGCTTGGACAAGGTTTGGCGAACGCCGTCGGGCTGGCGATAGCAGAGCGTACACTggc                                                                                                                          <  2:160106/150‑1 (MQ=255)
                             ggAAGAACTGAAGAACTTCCGTCAGTTGCATTCGAAAACCCCAGGCCACCCGGAGATTGGCTATACGCCAGGCGTTGAAACCACCACCGGCCCGCTTGGACAAGGTTTGGCGAACGCCGTCGGGCTGGCGATAGCAGAGCGTACACTgga                                                                                                                          <  2:1905300/150‑2 (MQ=255)
                             ggAAGAACTGAAGAACTTCCGTCAGTTGCATTCGAAAACCCCAGGCCACCCGGAGATTGGCTATACGCCAGGCGTTGAAACCACCACCGGCCCGCTTGGACAAGGTTTGGCGAACGCCGTAGGGCTGGCGATAGCAGAGCGTACACTggc                                                                                                                          >  1:3522272/1‑150 (MQ=255)
                                agaactgaagaactTCCGTCAGTTGCATTCGAAAACCCCAGGCCACCCGGAGATTGGCTATACGCCAGGCGTTGAAACCACCACCGGCCCGCTTGGACAAGGTTTGGCGAACGCCGTCGGGCTGGCGATAGCAGAGCGTACACTggcggc                                                                                                                       >  2:4266522/1‑150 (MQ=255)
                                agaactgaagaactTCCGTCAGTTGCATTCGAAAACCCCAGGCCACCCGGAGATTGGCTATACGCCAGGCGTTGAAACCACCACCGGCCCGCTTGGACAAGGTTTGGCGAACGCCGTCGGGCTGGCGATAGCAGAGCGTACACTggcggc                                                                                                                       <  1:4341199/150‑1 (MQ=255)
                                      gaagaactTCCGTCAGTTGCATTCGAAAACCCCAGGCCACCCGGAGATTGGCTATACGCCAGGCGTTGAAACCACCACCGGCCCGCTTGGACAAGGTTTGGCGAACGCCGTCGGGCTGGCGATAGCAGAGCGTACACTGGCGGCGCAGtt                                                                                                                 >  2:883369/1‑150 (MQ=255)
                                        agaactTCCGTCAGTTGCATTCGAAAACCCCAGGCCACCCGGAGATTGGCTATACGCCAGGCGTTGAAACCACCACCGGCCCGCTTGGACAAGGTTTGGCGAACGCCGTAGGGCTGGCGATAGCAGAGCGTACACTGGCGGCGCAGTTTa                                                                                                               >  1:4210405/1‑150 (MQ=255)
                                          aactTCCGTCAGTTGCATTCGAAAACCCCAGGCCACCCGGAGATTGGCTATACGCCAGGCGTTGAAACCACCACCGGCCCGCTTGGACAAGGTTTGGCGAACGCCGTAGGGCTGGCGATAGCAGAGCGTACACTGGCGGCGCAGTTTAAc                                                                                                             <  1:2327774/150‑1 (MQ=255)
                                           actTCCGTCAGTTGCATTCGAAAACCCCAGGCCACCCGGAGATTGGCTATACGCCAGGCGTTGAAACCACCACCGGCCCGCTTGGACAAGGTTTGGCGAACGCCGTCGGGCTGGCGATAGCAGAGTGTACACTGGCGGCGCAGTTTAAcc                                                                                                            <  1:3402826/150‑1 (MQ=255)
                                             ttCCGTCAGTTGCATTCGAAAACCCCAGGCCACCCGGAGATTGGCTATACGCCAGGCGTTGAAACCACCACCGGCCCGCTTGGACAAGGTTTGGCGAACGCCGTCGGGCTGGCGATAGCAGAGCGTACACTGGCGGCGCAGTTTAAccag                                                                                                          <  2:168412/150‑1 (MQ=255)
                                               ccGTCAGTTGCATTCGAAAACCCCAGGCCACCCGGAGATTGGCTATACGCCAGGCGTTGAAACCACCACCGGCCCGCTTGGACAAGGTTTGGCGAACGCCGTCGGGCTGGCGATAGCAGAGCGTACACTGGCGGCGCAGTTTAAccagcc                                                                                                        <  2:3818580/150‑1 (MQ=255)
                                                  tCAGTTGCATTCGAAAACCCCAGGCCACCCGGAGATTGGCTATACGCCAGGCGTTGAAACCACCACCGGCCCGCTTGGACAAGGTTTGGCGAACGCCGTAGGGCTGGCGATAGCAGAGCGTACACTGGCGGCGCAGTTTAACCAGCCAGa                                                                                                     >  1:4433049/1‑150 (MQ=255)
                                                   cAGTTGCATTCGAAAACCCCAGGCCACCCGGAGATTGGCTATACGCCAGGCGTTGAAACCACCACCGGCCCGCTTGGACAAGGTTTGGCGAACGCCGTCGGGCTGGCGATAGCAGAGCGTACACTGGCGGCGCAGTTTAACCAGCCAGAc                                                                                                    >  1:1253006/1‑150 (MQ=255)
                                                       tGCATTCGAAAACCCCAGGCCACCCGGAGATTGGCTATACGCCAGGCGTTGAAACCACCACCGGCCCGCTTGGACAAGGTTTGGCGAACGCCGTCGGGCTGGCGATAGCAGAGCGTACACTGGCGGCGCAGTTTAACCAGCCAGACCATg                                                                                                <  2:1062389/150‑1 (MQ=255)
                                                       tGCACTCGAAAACCCCAGGCCACCCGGAGATTGGCGATACGCCAGGCGTTGAAACCACCACCGGCCCGCTTGGACAAGGTTTGGCGAACGCCGTCGGGCTGGCGATAGCAGAGCGTACACTGGCGGCGCAGTTTAACCAGCCAGACCATg                                                                                                <  2:2646879/150‑1 (MQ=255)
                                                         cATTCGAAAACCCCAGGCCACCCGGAGATTGGCTATACGCCAGGCGTTGAAACCACCACCGGCCCGCTTGGACAAGGTTTGGCGAACGCCGTCGGGCTGGCGATAGCAGAGCGTACACTGGCGGCGCAGTTTAACCAGCCAGACCATgag                                                                                              >  1:2078548/1‑150 (MQ=255)
                                                          aTTCGAAAACCCCAGGCCACCCGGAGATTGGCTATACGCCAGGCGTTGAAACCACCACCGGCCCGCTTGGACAAGGTTTGGCGAACGCCGTCGGGCTGGCGATAGCAGAGCGTACACTGGCGGCGCAGTTTAACCAGCCAGACCATgaga                                                                                             >  1:3628071/1‑150 (MQ=255)
                                                            tCGAAAACCCCAGGCCACCCGGAGATTGGCTATACGCCAGGCGTTGAAACCACCACCGGCCCGCTTGGACAAGGTTTGGCGAACGCCGTCGGGCTGGCGATAGCAGAGCGTACACTGGCGGCGCAGTTTAACCAGCCAGACCATGAGAtc                                                                                           <  1:1844877/150‑1 (MQ=255)
                                                            tCGAAAACCCCAGGCCACCCGGAGATTGGCTATACGCCAGGCGTTGAAACCACCACCGGCCCGCTTGGACAAGGTTTGGCGAACGCCGTCGGGCTGGCGATAGCAGAGCGTACACTGGCGGCGCAGTTTAACCAGCCAGACCATGAGAtc                                                                                           <  2:782768/150‑1 (MQ=255)
                                                              gAAAACCCCAGGCCACCCGGAGATTGGCTATACGCCAGGCGTTGAAACCACCACCGGCCCGCTTGGACAAGGTTTGGCGAACGCCGTCGGGCTGGCGATAGCAGAGCGTACACTGGCGGCGCAGTTTAACCAGCCAGACCATGAGAtcgt                                                                                         >  2:4365022/1‑150 (MQ=255)
                                                               aaaaCCCCAGGCCACCCGGAGATTGGCTATACGCCAGGCGTTGAAACCACCACCGGCCCGCTTGGACAAGGTTTGGCGAACGCCGTCGGGCTGGCGATAGCAGAGCGTACACTGGCGGCGCAGTTTAACCAGCCAGACCATGAGAtcgtc                                                                                        <  2:703582/150‑1 (MQ=255)
                                                                 aaCCCCAGGCCACCCGGAGATTGGCTATACGCCAGGCGTTGAAACCACCACCGGCCCGCTTGGACAAGGTTTGGCGAACGCCGTCGGGCTGGCGATAGCAGAGCGTACACTGGCGGCGCAGTTTAACCAGCCAGACCATGAGATCGTCGa                                                                                      >  1:2219155/1‑150 (MQ=255)
                                                                 aaCCCCAGGCCACCCGGAGATTGGCTATACGCCAGGCGTTGAAACCACCACCGGCCCGCTTGGACAAGGTTTGGCGAACGCCGTCGGGCTGGCGATAGCAGAGCGTACACTGGCGGCGCAGTTTAACCAGCCAGACCATGAGATCGTCGa                                                                                      >  2:2617920/1‑150 (MQ=255)
                                                                  aCCCCAGGCCACCCGGAGATTGGCTATACGCCAGGCGTTGAAACCACCACCGGCCCGCTTGGACAAGGTTTGGCGAACGCCGTCGGGCTGGCGATAGCAGAGCGTACACTGGCGGCGCAGTTTAACCAGCCAGACCATGAGATCGTCGAt                                                                                     >  2:2131476/1‑150 (MQ=255)
                                                                   ccccAGGCCACCCGGAGATTGGCTATACGCCAGGCGTTGAAACCACCACCGGCCCGCTTGGACAAGGTTTGGCGAACGCCGTCGGGCTGGCGATAGCAGAGCGTACACTGGCGGCGCAGTTTAACCAGCCAGACCATGAGATCGTCGATc                                                                                    <  2:3327806/150‑1 (MQ=255)
                                                                    cccAGGCCACCCGGAGATTGGCTATACGCCAGGCGTTGAAACCACCACCGGCCCGCTTGGACAAGGTTTGGCGAACGCCGTAGGGCTGGCGATAGCAGAGCGTACACTGGCGGCGCAGTTTAACCAGCCAGACCATGAGATCGTCGATCa                                                                                   >  1:874214/1‑150 (MQ=255)
                                                                      cAGGCCACCCGGAGATTGGCTATACGCCAGGCGTTGAAACCACCACCGGCCCGCTTGGACAAGGTTTGGCGAACGCCGTCGGGCTGGCGATAGCAGAGCGTACACTGGCGGCGCAGTTTAACCAGCCAGACCATGAGATCGTCGATCACt                                                                                 >  2:2565016/1‑150 (MQ=255)
                                                                       aGGCCACCCGGAGATTGGCTATACGCCAGGCGTTGAAACCACCACCGGCCCGCTTGGACAAGGTTTGGCGAACGCCGTAGGGCTGGCGATAGCAGAGCGTACACTGGCGGCTCAGTTTAACCAGCCAGACCATGAGATCGTCGATCACtt                                                                                >  1:56576/1‑150 (MQ=255)
                                                                        ggCCACCCGGAGATTGGCTATACGCCAGGCGTTGAAACCACCACCGGCCCGCTTGGACAAGGTTTGGCGAACGCCGTCGGGCTGGCGATAGCAGAGCGTACACTGGCGGCGCAGTTTAACCAGCCAGACCATGAGATCGTCGATCACTTc                                                                               >  1:1639957/1‑150 (MQ=255)
                                                                            aCCCGGAGATTGGCTATACGCCAGGCGTTGAAACCACCACCGGCCCGCTTGGACAAGGTTTGGCGAACGCCGTCGGGCTGGCGATAGCAGAGCGTACACTGGCGGCGCAGTTTAACCAGCCAGACCATGAGATCGTCGATCACTTCACCt                                                                           <  2:1982134/150‑1 (MQ=255)
                                                                             cccGGAGATTGGCTATACGCCAGGCGTTGAAACCACCACCGGCCCGCTTGGACAAGGTTTGGCGAACGCCGTCGGGCTGGCGATAGCAGAGCGTACACTGGCGGCGCAGTTTAACCAGCCAGACCATGAGATCGTCGATCACTTCACCTa                                                                          <  1:3554009/150‑1 (MQ=255)
                                                                             cccGGAGATTGGCTATACGCCAGGCGTTGAAACCACCACCGGCCCGCTTGGACAAGGTTTGGCGAACGCCGTCGGGCTGGCGATAGCAGAGCGTACACTGGCGGCGCAGTTTAACCAGCCAGACCATGAGATCGTCGATCACTTCACCTa                                                                          >  2:1118259/1‑150 (MQ=255)
                                                                              ccGGAGATTGGCTATACGCCAGGCGTTGAATCCACCTCCGGCCCGCTTGGACAAGGTTTGGCGAACGCCGTAGGGCTGGCGATAGCAGAGCGTACACTGGCGGCGCAGTTTAACCAGCCAGACCATGAGATCGTCGATCACTTCACCTAt                                                                         <  1:4101870/150‑1 (MQ=255)
                                                                               cGGAGATTGGCTATACGCCAGGCGTTGAAACCACCACCGGCCCGCTTGGACAAGGTTTGGCGAACGCCGTAGGGCTGGCGATAGCAGAGCGTACACTGGCGGCGCAGTTTAACCAGCCAGACCATGAGATCGTCGATCACTTCACCTAtg                                                                        >  2:2183136/1‑150 (MQ=255)
                                                                                 gagaTTGGCTATACGCCAGGCGTTGAAACCACCACCGGCCCGCTTGGACAAGGTTTGGCGAACGCCGTAGGGCTGGCGATAGCAGAGCGTACACTGGCGGCGCAGTTTAACCAGCCAGACCATGAGATCGTCGATCACTTCACCTAtgtg                                                                      <  2:4442649/150‑1 (MQ=255)
                                                                                   gaTTGGCTATACGCCAGGCGTTGAAACCACCACCGGCCCGCTTGGACAAGGTTTGGCGAACGCCGTCGGGCTGGCGATAGCAGAGCGTACACTGGCGGCGCAGTTTAACCAGCCAGACCATGAGATCGTCGATCACTTCACCTATGTGtt                                                                    <  2:3687162/150‑1 (MQ=255)
                                                                                    aTTTGCTATACGCCAGGCGTTGAAACCACCACCGGCCCGCTTGGACAAGGTTTGGCGAACGCCGTCGGGCTGGCGATAGCAGAGCGTACACTGGCGGCGCAGTTTATCCAGCCAGTCCATTAGATCGTCGATCACTTCACCTATGTGttt                                                                   <  1:4539445/150‑1 (MQ=255)
                                                                                     ttGGCTATACGCCAGGCGTTGAAACCACCACCGGCCCGCTTGGACAAGGTTTGGCGAACGCCGTAGGGCTGGCGATAGCAGAGCGTACACTGGCGGCGCAGTTTAACCAGCCAGACCATGAGATCGTCGATCACTTCACCTATGTGTTTa                                                                  >  1:3886520/1‑150 (MQ=255)
                                                                                      tGGCTATACGCCAGGCGTTGAAACCACCACCGGCCCGCTTGGACAAGGTTTGGCGAACGCCGTCGGGCTGGCGATAGCAGAGCGTACACTGGCGGCGCAGTTTAACCAGCCAGACCATGAGATCGTCGATCACTTCACCTATGTGTTTAt                                                                 >  1:3097224/1‑150 (MQ=255)
                                                                                        gCTATACGCCAGGCGTTGAAACCACCACCGGCCCGCTTGGACAAGGTTTGGCGAACGCCGTCGGGCTGGCGATAGCAGAGCGTACACTGGCGGCGCAGTTTAACCAGCCAGACCATGAGATCGTCGATCACTTCACCTATGTGTTTATgg                                                               <  1:61899/150‑1 (MQ=255)
                                                                                        gCTATACGCCAGGCGTTGAAACCACCACCGGCCCGCTTGGACAAGGTTTGGCGAACGCCGTCGGGCTGGCGATAGCAGAGCGTACACTGGCGGCGCAGTTTAACCAGCCAGACCATGAGATCGTCGATCACTTCACCTATGTGTTTATgg                                                               <  1:2501783/150‑1 (MQ=255)
                                                                                         cTATACGCCAGGCGTTGAAACCACCACCGGCCCGCTTGGACAAGGTTTGGCGAACGCCGTAGGGCTGGCGATAGCAGAGCGTACACTGGCGGCGCAGTTTAACCAGCCAGACCATGAGATCGTCGATCACTTCACCTATGTGTTTATggg                                                              >  1:2993223/1‑150 (MQ=255)
                                                                                          tataCGCCAGGCGTTGAAACCACCACCGGCCCGCTTGGACAAGGTTTGGCGAACGCCGTCGGGCTGGCGATAGCAGAGCGTACACTGGCGGCGCAGTTTAACCAGCCAGACCATGAGATCGTCGATCACTTCACCTATGTGTTTATGGGc                                                             >  1:1436122/1‑150 (MQ=255)
                                                                                             aCGCCAGGCGTTGAAACCACCACCGGCCCGCTTGGACAAGGTTTGGCGAACGCCGTCGGGCTGGCGATAGCAGAGCGTACACTGGCGGCGCAGTTTAACCAGCCAGACCATGAGATCGTCGATCACTTCACCTATGTGTTTATGGGCGAc                                                          <  2:987916/150‑1 (MQ=255)
                                                                                                 cAGGCGTTGAAACCACCACCGGCCCGCTTGGACAAGGTTTGGCGAACGCCGTCGGGCTGGCGATAGCAGAGCGTACACTGGCGGCGCAGTTTAACCAGCCAGACCATGAGATCGTCGATCACTTCACCTATGTGTTTATGGGCGACGGCt                                                      >  1:1172771/1‑150 (MQ=255)
                                                                                                  aGGCGTTGAAACCACCACCGGCCCGCTTGGACAAGGTTTGGCGAACGCCGTAGGGCTGGCGATAGCAGAGCGTACACTGGCGGCGCAGTTTAACCAGCCAGACCATGAGATCGTCGATCACTTCACCTATGTGTTTATGGGCGACGGCTg                                                     <  1:4132348/150‑1 (MQ=255)
                                                                                                     cGTTGAAACCACCACCGGCCCGCTTGGACAAGGTTTGGCGAACGCCGTCGGGCTGGCGATAGCAGAGCGTACACTGGCGGCGCAGTTTAACCAGCCAGACCATGAGATCGTCGATCACTTCACCTATGTGTTTATGGGCGACGGCTGCCt                                                  <  2:2812213/150‑1 (MQ=255)
                                                                                                      gTTGAAACCACCACCGGCCCGCTTGGACAAGGTTTGGCGAACGCCGTCGGGCTGGCGATAGCAGAGCGTACACTGGCGGCGCAGTTTAACCAGCCAGACCATGAGATCGTCGATCACTTCACCTATGTGTTTATGGGCGACGGCTGCCTg                                                 >  1:239767/1‑150 (MQ=255)
                                                                                                        tGAAACCACCACCGGCCCGCTTGGACAAGGTTTGGCGAACGCCGTAGGGCTGGCGATAGCAGAGCGTACACTGGCGGCGCAGTTTAACCAGCCAGACCATGAGATCGTCGATCACTTCACCTATGTGTTTATGGGCGACGGCTGCCTGAt                                               >  2:2517489/1‑150 (MQ=255)
                                                                                                         gAAACCACCACCGGCCCGCTTGGACAAGGTTTGGCGAACGCCGTCGGGCTGGCGATAGCAGAGCGTACACTGGCGGCGCAGTTTAACCAGCCAGACCATGAGATCGTCGATCACTTCACCTATGTGTTTATGGGCGACGGCTGCCTGATg                                              >  2:3410103/1‑150 (MQ=255)
                                                                                                         gAAACCACCACCGGCCCGCTTGGACAAGGTTTGGCGAACGCCGTCGGGCTGGCGATAGCAGAGCGTACACTGGCGGCGCAGTTTAACCAGCCAGACCATGAGATCGTCGATCACTTCACCTATGTGTTTATGGGCGACGGCTGCCTGATg                                              >  2:346410/1‑150 (MQ=255)
                                                                                                           aaCCACCACCGGCCCGCTTGGACAAGGTTTGGCGAACGCCGTCGGGCTGGCGATAGCAGAGCGTACACTGGCGGCGCAGTTTAACCAGCCAGACCATGAGATCGTCGATCACTTCACCTATGTGTTTATGGGCGACGGCTGCCTGATGGa                                            >  2:3594146/1‑150 (MQ=255)
                                                                                                           aaCCACCACCGGCCCGCTTGGACAAGGTTTGGCGAACGCCGTCGGGCTGGCGATAGCAGAGCGTACACTGGCGGCGCAGTTTAACCAGCCAGACCATGAGATCGTCGATCACTTCACCTATGTGTTTATGGGCGACGGCTGCCTGATGGa                                            <  1:1342662/150‑1 (MQ=255)
                                                                                                            accaccaccGGCCCGCTTGGACAAGGTTTGGCGAACGCCGTCGGGCTGGCGATAGCAGAGCGTACACTGGCGGCGCAGTTTAACCAGCCAGACCATGAGATCGTCGATCACTTCACCTATGTGTTTATGGGCGACGGCTGCCTGATGGaa                                           <  1:2647627/150‑1 (MQ=255)
                                                                                                            accaccaccGGCCCGCTTGGACAAGGTTTGGCGAACGCCGTAGGGCTGGCGATAGCAGAGCGTACACTGGCGGCGCAGTTTAACCAGCCAGACCATGAGATCGTCGATCACTTCACCTATGTGTTTATGGGCGACGGCTGCCTGATGGaa                                           >  1:2231401/1‑150 (MQ=255)
                                                                                                                ccaccGGCCCGCTTGGACAAGGTTTGGCGAACGCCGTCGGGCTGGCGATAGCAGAGCGTACACTGGCGGCGCAGTTTAACCAGCCAGACCATGAGATCGTCGATCACTTCACCTATGTGTTTATGGGCGACGGCTGCCTGATGGAAGGTa                                       <  2:604382/150‑1 (MQ=255)
                                                                                                                  accGGCCCGCTTGGACAAGGTTTGGCGAACGCCGTCGGGCTGGCGATAGCAGAGCGTACACTGGCGGCGCAGTTTAACCAGCCAGACCATGAGATCGTCGATCACTTCACCTATGTGTTTATGGGCGACGGCTGCCTGATGGAAGGTAtt                                     >  1:2338432/1‑150 (MQ=255)
                                                                                                                    cGGCCCGCTTGGACAAGGTTTGGCGAACGCCGTCGGGCTGGCGATAGCAGAGCGTACACTGGCGGCGCAGTTTAACCAGCCAGACCATGAGATCGTCGATCACTTCACCTATGTGTTTATGGGCGACGGCTGCCTGATGGAAGGTATTTc                                   <  1:627383/150‑1 (MQ=255)
                                                                                                                       cccGCTTGGACAAGGTTTGGCGAACGCCGTCGGGCTGGCGATAGCAGAGCGTACACTGGCGGCGCAGTTTAACCAGCCAGACCATGAGATCGTCGATCACTTCACCTATGTGTTTATGGGCGACGGCTGCCTGATGGAAGGTATTTCCCa                                <  1:345772/150‑1 (MQ=255)
                                                                                                                        ccGCTTGGACAAGGTTTGGCGAACGCCGTCGGGCTGGCGATAGCAGAGCGTACACTGGCGGCGCAGTTTAACCAGCCAGACCATGAGATCGTCGATCACTTCACCTATGTGTTTATGGGCGACGGCTGCCTGATGGAAGGTATTTCCCAc                               <  2:2889342/150‑1 (MQ=255)
                                                                                                                        ccGCTTGGACAAGGTTTGGCGAACGCCGTAGGGCTGGCGATAGCAGAGCGTACACTGGCGGCGCAGTTTAACCAGCCAGACCATGAGATCGTCGATCACTTCACCTATGTGTTTATGGGCGACGGCTGCCTGATGGAAGGTATTTCCCAc                               <  1:1590935/150‑1 (MQ=255)
                                                                                                                          gCTTGGACAAGGTTTGGCGAACGCCGTCGGGCTGGCGATAGCAGAGCGTACACTGGCGGCGCAGTTTAACCAGCCAGACCATGAGATCGTCGATCACTTCACCTATGTGTTTATGGGCGACGGCTGCCTGATGGAAGGTATTTCCCACGa                             >  2:178023/1‑150 (MQ=255)
                                                                                                                          gCTTGGACAAGGTTTGGCGAACGCCGTCGGGCTGGCGATAGCAGAGCGTACACTGGCGGCGCAGTTTAACCAGCCAGACCATGAGATCGTCGATCACTTCACCTATGTGTGTATGGGCGACGGCTGCCTGATGGAAGGTATTTCCCACGa                             >  2:4462838/1‑150 (MQ=255)
                                                                                                                           cTTGGACAAGGTTTGGCGAACGCCGTCGGGCTGGCGATAGCAGAGCGTACACTGGCGGCGCAGTTTAACCAGCCAGACCATGAGATCGTCGATCACTTCACCTATGTGTTTATGGGCGACGGCTGCCTGATGGAAGGTATTTCCCACGaa                            >  2:3162330/1‑150 (MQ=255)
                                                                                                                           cTTGGACAAGGTTTGGCGAACGCCGTCGGGCTGGCGATAGCAGAGCGTACACTGGCGGCGCAGTTTAACCAGCCAGACCATGAGATCGTCGATCACTTCACCTATGTGTTTATGGGCGACGGCTGCCTGATGGAAGGTATTTCCCACGaa                            >  2:146741/1‑150 (MQ=255)
                                                                                                                           cTTGGACAAGGTTTGGCGAACGCCGTCGGGCTGGCGATAGCAGAGCGTACACTGGCGGCGCAGTTTAACCAGCCAGACCATGAGATCGTCGATCACTTCACCTATGTGTTTATGGGCGACGGCTGCCTGATGGAAGGTATTTCCCACGaa                            >  1:1470540/1‑150 (MQ=255)
                                                                                                                             tGGACAAGGTTTGGCGAACGCCGTCGGGCTGGCGATAGCAGAGCGTACACTGGCGGCGCAGTTTAACCAGCCAGACCATGAGATCGTCGATCACTTCACCTATGTGTTTATGGGCGACGGCTGCCTGATGGAAGGTATTTCCCACGAAGt                          >  1:1953606/1‑150 (MQ=255)
                                                                                                                             tGGACAAGGTTTGGCGAACGCCGTCGGGCTGGCGATAGCAGAGCGTACACTGGCGGCGCAGTTTAACCAGCCAGACCATGAGATCGTCGATCACTTCACCTATGTGTTTATGGGCGACGGCTGCCTGATGGAAGGTATTTCCCACGAAGt                          >  2:1429397/1‑150 (MQ=255)
                                                                                                                              ggACAAGGTTTGGCGAACGCCGTCGGGCTGGCGATAGCAGAGCGTACACTGGCGGCGCAGTTTAACCAGCCAGACCATGAGATCGTCGATCACTTCACCTATGTGTTTATGGGCGACGGCTGCCTGATGGAAGGTATTTCCCACGAAGTc                         <  2:491837/150‑1 (MQ=255)
                                                                                                                               gACAAGGTTTGGCGAACGCCGTCGGGCTGGCGATAGCAGAGCGTACACTGGCGGCGCAGTTTAACCAGCCAGACCATGAGATCGTCGATCACTTCACCTATGTGTTTATGGGCGACGGCTGCCTGATGGAAGGTATTTCCCACGAAGTCt                        >  2:1004320/1‑150 (MQ=255)
                                                                                                                                    ggTTTGGCGAACGCCGTCGGACTGGCGATAGCAGAGCGTACACTGGCGGCGCAGTTTAACCAGCCAGACCATGAGATCGTCGATCACTTCACCTATGTGTTTATGGGCGACGGCTGCCTGATGGAAGGTATTTCCCACGAAGTCTGTTCg                   >  1:400447/1‑150 (MQ=255)
                                                                                                                                     gTTTGGCGAACGCCGTCGGGCTGGCGATAGCAGAGCGTACACTGGCGGCGCAGTTTAACCAGCCAGACCATGAGATCGTCGATCACTTCACCTATGTGTTTATGGGCGACGGCTGCCTGATGGAAGGTATTTCCCACGAAGTCTGTTCGc                  >  1:3724342/1‑150 (MQ=255)
                                                                                                                                     gTTTGGCGAACGCCGTCGGGCTGGCGATAGCAGAGCGTACACTGGCGGCGCAGTTTAACCAGCCAGACCATGAGATCGTCGATCACTTCACCTATGTGTTTATGGGCGACGGCTGCCTGATGGAAGGTATTTCCCACGAAGTCTGTTCGc                  >  1:1360125/1‑150 (MQ=255)
                                                                                                                                     gTTTGGCGAACGCCGTCGGGCTGGCGATAGCAGAGCGTACACTGGCGGCGCAGTTTAACCAGCCAGACCATGAGATCGTCGATCACTTCACCTATGTGTTTATGGGCGACGGCTGCCTGATGGAAGGTATTTCCCACGAAGTCTGTTCGc                  >  1:2985696/1‑150 (MQ=255)
                                                                                                                                        tGGCGAACGCCGTCGGGCTGGCGATAGCAGAGCGTACACTGGCGGCGCAGTTTAACCAGCCAGACCATGAGATCGTCGATCACTTCACCTATGTGTTTATGGGCGACGGCTGCCTGATGGAAGGTATTTCCCACGAAGTCTGTTCGCTgg               <  2:535192/150‑1 (MQ=255)
                                                                                                                                           cGAACGCCGTCGGGCTGGCGATAGCAGAGCGTACACTGGCGGCGCAGTTTAACCAGCCAGACCATGAGATCGTCGATCACTTCACCTATGTGTTTATGGGCGACGGCTGCCTGATGGAAGGTATTTCCCACGAAGTCTGTTCGCTggcag            <  2:3762023/150‑1 (MQ=255)
                                                                                                                                               cGCCGTCGGGCTGGCGATAGCAGAGCGTACACTGGCGGCGCAGTTTAACCAGCCAGACCATGAGATCGTCGATCACTTCACCTAGGTGTTTATGGGCGACGGCTGCCTGATGGAAGGTATTTCCCACGAAGTCTGTTCGCTGGCAGGCAc        <  2:4050088/150‑1 (MQ=255)
                                                                                                                                                  cGTAGGGCTGGCGATAGCAGAGCGTACACTGGCGGCGCAGTTTAACCAGCCAGACCATGAGATCGTCGATCACTTCACCTATGTGTTTATGGGCGACGGCTGCCTGATGGAAGGTATTTCCCACGAAGTCTGTTCGCTGGCAGGCACGCt     >  1:1055580/1‑150 (MQ=255)
                                                                                                                                                   gTCGGGCTGGCGATAGCAGAGCGTACACTGGCGGCGCAGTTTAACCAGCCAGACCATGAGATCGTCGATCACTTCACCTATGTGTTTATGGGCGACGGCTGCCTGATGGAAGGTATTTCCCACGAAGTCTGTTCGCTGGCAGGCACGCTg    >  1:1824067/1‑150 (MQ=255)
                                                                                                                                                     cGGGCTGGCGATAGCAGAGCGTACACTGGCGGCGCAGTTTAACCAGCCAGACCATGAGATCGTCGATCACTTCACCTATGTGTTTATGGGCGACGGCTGCCTGATGGAAGGTATTTCCCACGAAGTCTGTTCGCTGGCAGGCACGCTggg  <  2:2413402/150‑1 (MQ=255)
                                                                                                                                                     cGGGCTGGCGATAGCAGAGCGTACACTGGCGGCGCAGTTTAACCAGCCAGACCATGAGATCGTCGATCACTTCACCTATGTGTTTATGGGCGACGGCTGCCTGATGGAAGGTATTTCCCACGAAGTCTGTTCGCTGGCAGGCACGCTggg  <  1:3275587/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
CTACATCTGACCGGTTACGACCTGCCGCTGGAAGAACTGAAGAACTTCCGTCAGTTGCATTCGAAAACCCCAGGCCACCCGGAGATTGGCTATACGCCAGGCGTTGAAACCACCACCGGCCCGCTTGGACAAGGTTTGGCGAACGCCGTCGGGCTGGCGATAGCAGAGCGTACACTGGCGGCGCAGTTTAACCAGCCAGACCATGAGATCGTCGATCACTTCACCTATGTGTTTATGGGCGACGGCTGCCTGATGGAAGGTATTTCCCACGAAGTCTGTTCGCTGGCAGGCACGCTGGG  >  CP009273/2573214‑2573512

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A