Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F121 I0 R1 856 273.6 9081716 94.1% 8545894 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,088,065 C→A 7.9% V125V (GTG→GTT)  yggR ← putative PilT family AAA+ ATPase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,088,0650CA 7.9% 1093.6 / 49.1 289V125V (GTG→GTT) yggRputative PilT family AAA+ ATPase
Reads supporting (aligned to +/- strand):  ref base C (137/129);  new base A (9/14);  total (146/143)
Fisher's exact test for biased strand distribution p-value = 2.83e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

Only 100 of 297 total aligned reads displayed.

ATTTCCCGCTGCTGGATCAAACATCGCTGGCTGGCATAGAGATATTCAACAGGATCTTCCAGCGTCAGAATATGCGCATCGGCATGTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTCACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTGGTGCGCCAAGCTGTTCGAGCTGCGGGCAGTGCGAAGGTAACAGCCGTAACGCCAGCGAAATGCCATGCCGTTGCGCGAATGCGCTGCCACGCAATCGCTG  >  CP009273/3087916‑3088214
                                                                                                                                                     |                                                                                                                                                     
agttCCCGCTGCTGGATCAAACATCGCTGGCTGGCATAGAGATATTCAACAGGATCTTCCAGCGTCAGAATATGCGCATCGGCATGTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTc                                                                                                                                                       <  2:1331193/148‑1 (MQ=255)
aTTTCCCGCTGCTGGATCAAACATCGCTGGCTGGCATAGAGATATTCAACAGGATCTTCCAGCGTCAGAATATGCGCATCGGCATGTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTc                                                                                                                                                       >  1:908495/1‑150 (MQ=255)
  ttCCCGCTGCTGGATCAAACATCGCTGGCTGGCATAGAGATATTCAACAGGATCTTCCAGCGTCAGAATATGCGCATCGGCATGTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTCAc                                                                                                                                                     >  1:1868889/1‑150 (MQ=255)
        ctgctgGATCAAACATCGCTGGCTGGCATAGAGATATTCAACAGGATCTTCCAGCGTCAGAATATGCGCATCGGCATGTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTCACCAGAAt                                                                                                                                               >  2:29810/1‑150 (MQ=255)
        ctgctgGATCAAACATCGCTGGCTGGCATAGAGATATTCAACAGGATCTTCCAGCGTCAGAATATGCGCATCGGCATGTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTCACCAGAAt                                                                                                                                               <  1:915887/150‑1 (MQ=255)
        ctgctgGATCAAACATCGCTGGCTGGCATAGAGATATTCAACAGGATCTTCCAGCGTCAGAATATGCGCATCGGCATGTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTCACCAGAAt                                                                                                                                               <  1:1361518/150‑1 (MQ=255)
           ctgGATCAAACATCGCTGGCTGGCATAGAGATATTCAACAGGATCTTCCAGCGTCAGAATATGCGCATCGGCATGTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTCACCAGAATCAg                                                                                                                                            >  2:4336506/1‑150 (MQ=255)
           ctgGATCAAACATCGCTGGCTGGCATAGAGATATTCAACAGGATCTTCCAGCGTCAGAATATGCGCATCGGCATGTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTAACCAGAATCAg                                                                                                                                            >  2:3968434/1‑150 (MQ=255)
             ggATCAAACATCGCTGGCTGGCATAGAGATATTCAACAGGATCTTCCAGCGTCAGAATATGCGCATCGGCATGTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTCACCAGAATCAGGc                                                                                                                                          <  2:71378/150‑1 (MQ=255)
              gATCAAACATCGCTGGCTGGCATAGAGATATTCAACAGGATCTTCCAGCGTCAGAATATGCGCATCGGCATGTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTCACCAGAATCAGGcc                                                                                                                                         >  2:3534081/1‑150 (MQ=255)
                 cAAACATCGCTGGCTGGCATAGAGATATTCAACAGGATCTTCCAGCGTCAGAATATGCGCATCGGCATGTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTCACCAGAATCAGGCCAtt                                                                                                                                      >  1:139569/1‑150 (MQ=255)
                  aaaCATCGCTGGCTGGCATAGAGATATTCAACAGGATCTTCCAGCGTCAGAATATGCGCATCGGCATGTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTCACCAGAATCAGGCCATtc                                                                                                                                     <  2:1923151/150‑1 (MQ=255)
                     cATCGCTGGCTGGCATAGAGATATTCAACAGGATCTTCCAGCGTCAGAATATGCGCATCGGCATGTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTCACCAGAATCAGGCCATTCTCg                                                                                                                                  <  2:320636/150‑1 (MQ=255)
                         gctggctgGCATAGAGATATTCAACAGGATCTTCCAGCGTCAGAATATGCGCATCGGCATGTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTCACCAGAATCAGGCCATTCTCGctct                                                                                                                              <  1:3530871/150‑1 (MQ=255)
                          ctggctggCATAGAGATATTCAACAGGATCTTCCAGCGTCAGAATATGCGCATCGGCATGTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTCACCAGAATCAGGCCATTCTCGCTCtt                                                                                                                             <  1:533695/150‑1 (MQ=255)
                          ctggctggCATAGAGATATTCAACAGGATCTTCCAGCGTCAGAATATGCGCATCGGCATGTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTCACCAGAATCAGGCCATTCTCGCTCtt                                                                                                                             <  2:893439/150‑1 (MQ=255)
                             gctggcATAGAGATATTCAACAGGATCTTCCAGCGTCAGAATATGCGCATCGGCATGTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTCACCAGAATCAGGCCATTCTCGCTCTTGAg                                                                                                                          >  2:1112751/1‑150 (MQ=255)
                              ctggcATAGAGATATTCAACAGGATCTTCCAGCGTCAGAATATGCGCATCGGCATGTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTCACCAGAATCAGGCCATTCTCGCTCTTGAGt                                                                                                                         >  1:1223879/1‑150 (MQ=255)
                              ctggcATAGAGATATTCAACAGGATCTTCCAGCGTCAGAATATGCGCATCGGCATGTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTCACCAGAATCAGGCCATTCTCGCTCTTGAGt                                                                                                                         >  1:4501986/1‑150 (MQ=255)
                              ctggcATAGAGATATTCAACAGGATCTTCCAGCGTCAGAATATGCGCATCGGCATGTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTAACCAGAATCAGGCCATTCTCGCTCTTGAGt                                                                                                                         <  1:3326591/150‑1 (MQ=255)
                                ggcATAGAGATATTCAACAGGATCTTCCAGCGTCAGAATATGCGCATCGGCATGTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTCACCAGAATCAGGCCATTCTCGCTCTTGAGTaa                                                                                                                       >  2:2437753/1‑150 (MQ=255)
                                  cATAGAGATATTCAACAGGATCTTCCAGCGTCAGAATATGCGCATCGGCATGTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTCACCAGAATCAGGCCATTCTCGCTCTTGAGTAAtt                                                                                                                     <  1:2691318/150‑1 (MQ=255)
                                   aTAGAGATATTCAACAGGATCTTCCAGCGTCAGAATATGCGCATCGGCATGTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTCACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTc                                                                                                                    <  2:954531/150‑1 (MQ=255)
                                              cAACAGGATCTTCCAGCGTCAGAATATGCGCATCGGCATGTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTCACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCg                                                                                                         <  1:1835291/150‑1 (MQ=255)
                                               aaCAGGATCTTCCAGCGTCAGAATATGCTCATCGGCATGTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGTTTTGCCACTCCCCGTCGCCCCCGTCACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATAcctt                                                                                                        <  1:945699/150‑3 (MQ=255)
                                                aCAGGATCTTCCAGCGTCAGAATATGCGCATCGGCATGTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTCACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGtt                                                                                                       >  1:2242411/1‑150 (MQ=255)
                                                 cAGGATCTTCCAGCGTCAGAATATGCGCATCGGCATGTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTCACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTg                                                                                                      <  1:4157800/150‑1 (MQ=255)
                                                 cAGGATCTTCCAGCGTCAGAATATGCGCATCGGCATGTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTCACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTg                                                                                                      <  2:3177908/150‑1 (MQ=255)
                                                   ggATCTTCCAGCGTCAGAATATGCGCATCGGCATGTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTCACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTGGt                                                                                                    <  1:2468211/150‑1 (MQ=255)
                                                         tCCAGCGTCAGAATATGCGCATCGGCATGTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTCACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTGGTGCGCCa                                                                                              <  2:29333/150‑1 (MQ=255)
                                                         tCCAGCGTCAGAATATGCGCATCGGCATGTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTCACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTGGTGCGCCa                                                                                              <  2:2693807/150‑1 (MQ=255)
                                                           cAGCGTCAGAATATGCGCATCGGCATGTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTCACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTGGTGCGCCAAg                                                                                            <  2:3195166/150‑1 (MQ=255)
                                                             gCGTCAGAATATGCGCATCGGCATGTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTAACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTGGTGCGCCAAGCt                                                                                          <  1:548532/150‑1 (MQ=255)
                                                               gTCAGAATATGCGCATCGGCATGTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTAACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTGGTGCGCCAAGCTGt                                                                                        >  1:2908945/1‑150 (MQ=255)
                                                                tCAGAATATGCGCATCGGCATGTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTCACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTGGTGCGCCAAGCTGtt                                                                                       <  1:2788177/150‑1 (MQ=255)
                                                                     atatGCGCATCGGCATGTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTCACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTGGTGCGCCAAGCTGTTCGAGc                                                                                  <  2:3609117/150‑1 (MQ=255)
                                                                     atatGCGCATCGGCATGTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTCACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTGGTGCGCCAAGCTGTTCGAGc                                                                                  >  1:2795846/1‑150 (MQ=255)
                                                                      tatGCGCATCGGCATGTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTCACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTGGTGCGCCAAGCTGTTCGAGCt                                                                                 >  2:1526464/1‑150 (MQ=255)
                                                                          cgcATCGGCATGTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTCACCAGAATCAGGCAATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTGGTGCGCCAAGCTGTTCGAGCTGCgg                                                                             >  1:3549168/1‑150 (MQ=255)
                                                                             aTCGGCATGTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTCACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTGGTGCGCCAAGCTGTTCGAGCTGCGGGCa                                                                          >  2:3054367/1‑150 (MQ=255)
                                                                              tCGGCATGTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTCACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTGGTGCGCCAAGCTGTTCGAGCTGCGGGCAg                                                                         <  1:3623707/150‑1 (MQ=255)
                                                                                ggCATGTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTCACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTGGTGCGCCAAGCTGTTCGAGCTGCGGGCAGTg                                                                       <  1:1576196/150‑1 (MQ=255)
                                                                                ggCATGTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTCACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTGGTGCGCCAAGCTGTTCGAGCTGCGGGCAGTg                                                                       >  1:2168706/1‑150 (MQ=255)
                                                                                  cATGTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTCACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTGGTGCGCCAAGCTGTTCGAGCTGCGGGCAGTGCg                                                                     >  1:812624/1‑150 (MQ=255)
                                                                                  cATGTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTCACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTGGTGCGCCAAGCTGTTCGAGCTGCGGGCAGTGCg                                                                     >  1:2731165/1‑150 (MQ=255)
                                                                                   aTGTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTCACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTGGTGCGCCAAGCTGTTCGAGCTGCGGGCAGTGCGa                                                                    >  1:195803/1‑150 (MQ=255)
                                                                                   aTGTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTAACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTGGTGCGCCAAGCTGTTCGAGCTGCGGGCAGTGCGa                                                                    >  1:3467201/1‑150 (MQ=255)
                                                                                     gTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTAACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTGGTGCGCCAAGCTGTTCGAGCTGCGGGCAGTGCGAAg                                                                  <  1:1041356/150‑1 (MQ=255)
                                                                                      ttgattgaGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTCACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTGGTGCGCCAAGCTGTTCGAGCTGCGGGCAGTGCGAAgg                                                                 <  2:2589144/150‑1 (MQ=255)
                                                                                      ttgattgaGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTCACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTGGTGCGCCAAGCTGTTCGAGCTGCGGGCAGTGCGAAgg                                                                 >  2:2263111/1‑150 (MQ=255)
                                                                                      ttgattgaGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTCACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTGGTGCGCCAAGCTGTTCGAGCTGCGGGCAGTGCGAAgg                                                                 <  2:524928/150‑1 (MQ=255)
                                                                                       tgattgaGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTCACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTGGTGCGCCAAGCTGTTCGAGCTGCGGGCAGTGCGAAGGt                                                                >  2:2396659/1‑150 (MQ=255)
                                                                                        gattgaGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTCACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTGGTGCGCCAAGCTGTTCGAGCTGCGGGCAGTGCGAAGGTa                                                               >  1:2982514/1‑150 (MQ=255)
                                                                                         attgaGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTCACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTGGTGCGCCAAGCTGTTCGAGCTGCGGGCAGTGCGAAGGTaa                                                              <  1:1700765/150‑1 (MQ=255)
                                                                                          ttgaGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTCACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTGGTGCGCCAAGCTGTTCGAGCTGCGGGCAGTGCGAAGGTAAc                                                             <  2:188295/150‑1 (MQ=255)
                                                                                          ttgaGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTCACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTGGTGCGCCAAGCTGTTCGAGCTGCGGGCAGTGCGAAGGTAAc                                                             <  2:3689389/150‑1 (MQ=255)
                                                                                          ttgaGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTCACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTGGTGCGCCAAGCTGTTCGAGCTGCGGGCAGTGCGAAGGTAAc                                                             <  1:961892/150‑1 (MQ=255)
                                                                                           tgaGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTCACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTGGTGCGCCAAGCTGTTCGAGCTGCGGGCAGTGCGAAGGTAACa                                                            <  1:4356618/150‑1 (MQ=255)
                                                                                            gagaTAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTCACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTGGTGCGCCAAGCTGTTCGAGCTGCGGGCAGTGCGAAGGTAACAg                                                           <  1:1317932/150‑1 (MQ=255)
                                                                                             agaTAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTCACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTGGTGCGCCAAGCTGTTCGAGCTGCGGGCAGTGCGAAGGTAACAGc                                                          >  2:4117073/1‑150 (MQ=255)
                                                                                              gaTAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTCACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTGGTGCGCCAAGCTGTTCGAGCTGCGGGCAGTGCGAAGGTAACAGcc                                                         <  1:308711/150‑1 (MQ=255)
                                                                                               aTAGTCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTCACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTGGTGCGCCAAGCTGTTCGAGCTGCGGGCAGTGCGAAGGTAACAGCCg                                                        >  2:1405015/1‑150 (MQ=255)
                                                                                                 aGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTCACCAGAATCAGGCCATTCTCGCGCTTGAGTCATTCCGGCAATACCGTTGGTGCGCCAAGCTGTTCGAGCTGCGGGCAGTGCGAAGGTCACAGCCGTa                                                      >  1:3945193/1‑150 (MQ=255)
                                                                                                 aGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTAACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTGGTGCGCCAAGCTGTTCGAGCTGCGGGCAGTGCGAAGGTAACAGCCGTa                                                      >  2:3116875/1‑150 (MQ=255)
                                                                                                   ccAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTCACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTGGTGCGCCAAGCTGTTCGAGCTGCGGGCAGTGCGAAGGTAACAGCCGTAAc                                                    >  2:958869/1‑150 (MQ=255)
                                                                                                   ccAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTCACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTGGTGCGCCAAGCTGTTCGAGCTGCGGGCAGTGCGAAGGTAACAGCCGTAAc                                                    <  1:3729004/150‑1 (MQ=255)
                                                                                                    cAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTCACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTGGTGCGCCAAGCTGTTCGAGCTGCGGGCAGTGCGAAGGTAACAGCCGTAACg                                                   >  1:112523/1‑150 (MQ=255)
                                                                                                     aaacATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGACACCAGAATCAGGCCATTATCGCTCTAGAGTAATTCCGGCAATACCGTTGGTGAGCCAATCTGTTAGAGCTGCGGGCAGTCCGAAGGTAACAGCCGTAACGc                                                  >  1:4396463/4‑150 (MQ=255)
                                                                                                     aaCCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTCACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTGGTGCGCCAAGCTGTTCGAGCTGCGGGCAGTGCGAAGGTAACAGCCGTAACGc                                                  >  1:125553/1‑150 (MQ=255)
                                                                                                       ccATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTCACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTGGTGCGCCAAGCTGTTCGAGCTGCGGGCAGTGCGAAGGTAACAGCCGTAACGCCa                                                >  1:2918760/1‑150 (MQ=255)
                                                                                                         aTCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTCACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTGGTGCGCCAAGCTGTTCGAGCTGCGGGCAGTGCGAAGGTAACAGCCGTAACGCCAGc                                              <  2:1585498/150‑1 (MQ=255)
                                                                                                              cgccAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTCACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTGGTGCGCCAAGCTGTTCGAGCTGCGGGCAGTGCGAAGGTAACAGCCGTAACGCCAGCGAAat                                         >  1:1478325/1‑150 (MQ=255)
                                                                                                                ccAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTCACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTGGTGCGCCAAGCTGTTCGAGCTGCGGGCAGTGCGAAGGTAACAGCCGTAACGCCAGCGAAatgc                                       >  1:3236032/1‑150 (MQ=255)
                                                                                                                 cAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTCACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTGGTGCGCCAAGCTGTTCGAGCTGCGGGCAGTGCGAAGGTAACAGCCGTAACGCCAGCGAAatgcc                                      <  1:3183382/150‑1 (MQ=255)
                                                                                                                 cAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTCACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTGGTGCGCCAAGCTGTTCGAGCTGCGGGCAGTGCGAAGGTAACAGCCGTAACGCCAGCGAAatgcc                                      <  2:1711626/150‑1 (MQ=255)
                                                                                                                    cGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTCACCAGAATCAGGCCAGTCTCGCTCTTGAGTACTTCCGGCAATACCGTTGGTGCGCCAAGCTGTTCGAGCTGCGGGCAGTGCGAAGGTAACAGCCGTAACGCCAGCGAAatgccatg                                   <  2:2788325/150‑1 (MQ=255)
                                                                                                                    cGTGGTAGATTTGCCACGCCCCGTCGCCCCCGTCACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTGGTGCGCCAAGCTGTTCGAGCTGCGGGCAGTGCGAAGGTAACAGCCGTAACGCCAGCGAAatgccatg                                   <  2:2324225/150‑1 (MQ=255)
                                                                                                                     gTGGTAGATTTGCCACTCCCCGTCGCCCCCGTCACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTGGTGCGCCAAGCTGTTCGAGCTGCGGGCAGTGCGAAGGTAACAGCCGTAACGCCAGCGAAatgccatgc                                  <  1:764914/150‑1 (MQ=255)
                                                                                                                     gTGGTAGATTTGCCACTCCCCGTCGCCCCCGTCACCAGAATCAGGCCAGTCTCGCTCTTGAGTAATTCCGGCAATACCGTTGGTGCGCCAAGCTGTTCGAGCTGCGGGCAGTGCGAAGGTAACAGCCGTAACGCCAGCGAAatgccatgc                                  <  2:3248620/150‑1 (MQ=255)
                                                                                                                      tGGTAGATTTGCCACTCCCCGTCGCCCCCGTCACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTGGTGCGCCAAGCTGTTCGAGCTGCGGGCAGTGCGAAGGTAACAGCCGTAACGCCAGCGAAatgccatgcc                                 <  2:3434430/150‑1 (MQ=255)
                                                                                                                       ggTAGATTTGCCACTCCCCGTCGCCCCCGTCACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTGGTGCGCCAAGCTGTTCGAGCTGCGGGCAGTGCGAAGGTAACAGCCGTAACGCCAGCGAAATGCCATGCCg                                >  1:3794621/1‑150 (MQ=255)
                                                                                                                       ggTAGATTTGCCACTCCCCGTCGCCCCCGTCACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTGGTGCGCCAAGCTGTTCGAGCTGCGGGCAGTGCGAAGGTAACAGCCGTAACGCCAGCGAAATGCCATGCCg                                >  2:4409536/1‑150 (MQ=255)
                                                                                                                        gTAGATTTGCCACTCCCCGTCGCCCCCGTCACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTGGTGCGCCAAGCTGTTCGAGCTGCGGGCAGTGCGAAGGTAACAGCCGTAACGCCAGCGAAATGCCATGCCGt                               >  2:4024599/1‑150 (MQ=255)
                                                                                                                          aGATTTGCCACTCCCCGTCGCCCCCGTCACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTGGTGCGCCAAGCTGTTCGAGCTGCGGGCAGTGCGAAGGTAACAGCCGTAACGCCAGCGAAATGCCATGCCGTTg                             >  1:2646336/1‑150 (MQ=255)
                                                                                                                           gATTTGCCACTCCCCGTCGCCCCCGTCACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTGGTGCGCCAAGCTGTTCGAGCTGCGGGCAGTGCGAAGGTAACAGCCGTAACGCCAGCGAAATGCCATGCCGTTgc                            >  2:1335829/1‑150 (MQ=255)
                                                                                                                               tGCCACTCCCCGTCGCCCCCGTCACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTGGTGCGCCAAGCTGTTCGAGCTGCGGGCAGTGCGAAGGTAACAGCCGTAACGCCAGCGAAATGCCATGCCGTTGCGCGa                        <  1:4439709/150‑1 (MQ=255)
                                                                                                                                gCCACTCCCCGTCGCCCCCGTCACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTGGTGCGCCAAGCTGTTCGAGCTGCGGGCAGTGCGAAGGTAACAGCCGTAACGCCAGCGAAATGCCATGCCGTTGCGCGaa                       >  2:1796342/1‑150 (MQ=255)
                                                                                                                                 ccACTCCCCGTCGCCCCCGTCACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTGGTGCGCCAAGCTGTTCGAGCTGCGGGCAGTGCGAAGGTAACAGCCGTAACGCCAGCGAAATGCCATGCCGTTGCGCGAAt                      >  1:2535178/1‑150 (MQ=255)
                                                                                                                                      ccccGTCGCCCCCGTCACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTGGTGCGCCAAGCTGTTCGAGCTGCGGGCAGTGCGAAGGTAACAGCCGTAACGCCAGCGAAATGCCATGCCGTTGCGCGAATGCGCt                 <  2:3812250/150‑1 (MQ=255)
                                                                                                                                      ccccGTCGCCCCCGTCACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTGGTGCGCCAAGCTGTTCGAGCTGCGGGCAGTGCGAAGGTAACAGCCGTAACGCCAGCGAAATGCCATGCCGTTGCGCGAATGCGCt                 >  1:2818033/1‑150 (MQ=255)
                                                                                                                                          gTCGCCCCCGTCACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTGGTGCGCCAAGCTGTTCGAGCTGCGGGCAGTGCGAAGGTAACAGCCGTAACGCCAGCGAAATGCCATGCCGTTGCGCGAATGCGCTGCCa             >  1:2311494/1‑150 (MQ=255)
                                                                                                                                           tCGCCCCCGTCACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTGGTGCGCCAAGCTGTTCGAGCTGCGGGCAGTGCGAAGGTAACAGCCGTAACGCCAGCGAAATGCCATGCCGTTGCGCGAATGCGCTGCCAc            >  2:2884948/1‑150 (MQ=255)
                                                                                                                                             gCCCCCGTCACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTGGTGCGCCAAGCTGTTCGAGCTGCGGGCAGTGCGAAGGTAACAGCCGTAACGCCAGCGAAATGCCATGCCGTTGCGCGAATGCGCTGCCACGc          >  1:4477578/1‑150 (MQ=255)
                                                                                                                                             gCCCCCGTCACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTGGTGCGCCAAGCTGTTCGAGCTGCGGGCAGTGCGAAGGTAACAGCCGTAACGCCAGCGAAATGCCATGCCGTTGCGCGAATGCGCTGCCACGc          >  1:3805403/1‑150 (MQ=255)
                                                                                                                                              cccccGTCACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTGGTGCGCCAAGCTGTTCGAGCTGCGGGCAGTGCGAAGGTAACAGCCGTAACGCCAGCGAAATGCCATGCCGTTGCGCGAATGCGCTGCCACGCa         >  1:4225721/1‑150 (MQ=255)
                                                                                                                                               ccccGTCACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTGGTGCGCCAAGCTGTTCGAGCTGCGGGCAGTGCGAAGGTAACAGCCGTAACGCCAGCGAAATGCCATGCCGTTGCGCGAATGCGCTGCCACGCaa        >  2:330577/1‑150 (MQ=255)
                                                                                                                                                    tCACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTGGTGCGCCAAGCTGTTCGAGCTGCGGGCAGTGCGAAGGTAACAGCCGTAACGCCAGCGAAATGCCATGCCGTTGCGCGAATGCGCTGCCACGCAATCGCt   <  1:3041505/150‑1 (MQ=255)
                                                                                                                                                    tCACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTGGTGCGCCAAGCTGTTCGAGCTGCGGGCAGTGCGAAGGTAACAGCCGTAACGCCAGCGAAATGCCATGCCGTTGCGCGAATGCGCTGCCACGCAATCGCt   >  1:697704/1‑150 (MQ=255)
                                                                                                                                                     cACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTGGTGCGCCAAGCTGTTCGAGCTGCGGGCAGTGCGAAGGTAACAGCCGTAACGCCAGCGAAATGCCATGCCGTTGCGCGAATGCGCTGCCACGCAATCGCTg  <  1:3589510/150‑1 (MQ=255)
                                                                                                                                                     cACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTGGTGCGCCAAGCTGTTCGAGCTGCGGGCAGTGCGAAGGTAACAGCCGTAACGCCAGCGAAATGCCATGCCGTTGCGCGAATGCGCTGCCACGCAATCGCTg  >  1:150767/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
ATTTCCCGCTGCTGGATCAAACATCGCTGGCTGGCATAGAGATATTCAACAGGATCTTCCAGCGTCAGAATATGCGCATCGGCATGTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTCACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTGGTGCGCCAAGCTGTTCGAGCTGCGGGCAGTGCGAAGGTAACAGCCGTAACGCCAGCGAAATGCCATGCCGTTGCGCGAATGCGCTGCCACGCAATCGCTG  >  CP009273/3087916‑3088214

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A