Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F121 I0 R1 856 273.6 9081716 94.1% 8545894 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,246,634 A→T 5.2% intergenic (+8/+43) yhaI → / ← yhaJ DUF805 family inner membrane protein/LysR family putative transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,246,6340AT 5.2% 738.1 / 22.7 212intergenic (+8/+43)yhaI/yhaJDUF805 family inner membrane protein/LysR family putative transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base A (84/117);  new base T (2/9);  total (86/126)
Fisher's exact test for biased strand distribution p-value = 2.06e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.61e-01

Only 100 of 215 total aligned reads displayed.

CGATACCGACCGTTCAGGTGCCTGGGCCTTGCTCTTTTTTGTTCCGTTTATTGGCTGGCTCGTGCTTCTGGTTTTTTTCTGCACAGAAGGTACTTCTGGCAGCAATCGTTACGGAAACGATCCGAAGTTTGGTTCAAATTAATCTTAGAATTGGGGCGATATTTCGCCCCTTTTTATTAACAATAATAAATATTATTTTCCGTTAAAAAGTTTGGGAATTTCCCGCAGACACCAGGATTTCGCTTCCCCCATACTGTCACGACGCCAGGCCATAATAATATCGATCTCGCTGGTCGATT  >  CP009273/3246485‑3246783
                                                                                                                                                     |                                                                                                                                                     
 gATACCGACCGTTCAGGTGCCTGGGCCTTGCTCTTTTTTGTTCCGTTTATTGGCTGGCTCGTGCTTCTGGTTTTTTTCTGCACAGAAGGTACTTCTGGCAGCAATCGTTACGGAAACGATCCGAAGTTTGGTTCAAATTAATCTTAGAAt                                                                                                                                                      >  1:2837324/1‑150 (MQ=255)
 gATACCGACCGTTCAGGTGCCTGGGCCTTGCTCTTTTTTGTTCCGTTTATTGGCTGGCTCGTGCTTCTGGTTTTTTTCTGCACAGAAGGTACTTCTGGCAGCAATCGTTACGGAAACGATCCGAAGTTTGGTTCAAATTAATCTTAGAAt                                                                                                                                                      <  1:1753074/150‑1 (MQ=255)
  aTACCGACCGTTCAGGTGCCTGGGCCTTGCTCTTTTTTGTTCCGTTTATTGGCTGGCTCGTGCTTCTGGTTTTTTACTGCACAGAAGGTACTTCTGGCAGCAATCGTTACGGAAACGATCCGAAGTTTGGTTCAAATTAATCTTAGAAtt                                                                                                                                                     <  2:4029397/150‑1 (MQ=255)
      cgaccgTTCAGGTGCCTGGGCCTTGCTCTTTTTTGTTCCGTTTATTGGCTGGCTCGTGCTTCTGGTTTTTTTCTGCACAGAAGGTACTTCTGGCAGCAATCGTTACGGAAACGATCCGAAGTTTGGTTCAAATTAATCTTAGATTTgggg                                                                                                                                                 <  1:356885/150‑1 (MQ=255)
      cgaccgTTCAGGTGCCTGGGCCTTGCTCTTTTTTGTTCCGTTTATTGGCTGGCTCGTGCTTCTGGTTTTTTTCTGCACAGAAGGTACTTCTGGCAGCAATCGTTACGGAAACGATCCGAAGTTTGGTTCAAATTAATCTTAGAATTgggg                                                                                                                                                 <  1:2419510/150‑1 (MQ=255)
       gaccgTTCAGGTGCCTGGGCCTTGCTCTTTTTTGTTCCGTTTATTGGCTGGCTCGTGCTTCTGGTTTTTTTCTGCACAGAAGGTACTTCTGGCAGCAATCGTTACGGAAACGATCCGAAGTTTGGTTCAAATTAATCTTAGATTTGGGGc                                                                                                                                                <  2:851230/150‑1 (MQ=255)
        accgTTCTGGTGCCTGGGCCTTGCTCTTTTTTGTTCCGTTTATTGGCTGGCTCGTGCTTCTGGTTTTTTTCTGCACAGAAGGTACTTCTGGCAGCAATCGTTACGGAAACGATCCGAAGTTTGGTTCAAATTAATCTTAGAATTGGGGCg                                                                                                                                               <  1:2175647/150‑1 (MQ=255)
        accgTTCAGGTGCCTGGGCCTTGCTCTTTTTTGTTCCGTTTATTGGCTGGCTCGTGCTTCTGGTTTTTTTCTGCACAGAAGGTACTTCTGGCAGCAATCGTTACGGAAACGATCCGAAGTTTGGTTCAAATTAATCTTAGAATTGGGGCg                                                                                                                                               <  2:73607/150‑1 (MQ=255)
        accgTTCAGGTGCCTGGGCCTTGCTCTTTTTTGTTCCGTTTATTGGCTGGCTCGTGCTTCTGGTTTTTTTCTGCACAGAAGGTACTTCTGGCAGCAATCGTTACGGAAACGATCCGAAGTTTGGTTCAAATTAATCTTAGAATTGGGGCg                                                                                                                                               <  1:1622839/150‑1 (MQ=255)
          cgTTCAGGTGCCTGGGCCTTGCTCTTTTTTGTTCCGTTTATTGGCTGGCTCGTGCTTCTGGTTTTTTTCTGCACAGAAGGTACTTCTGGCAGCAATCGTTACGGAAACGATCCGAAGTTTGGTTCAAATTAATCTTAGAATTGGGGCGat                                                                                                                                             >  1:2739315/1‑150 (MQ=255)
          cgTTCAGGTGCCTGGGCCTTGCTCTTTTTTGTTCCGTTTATTGGCTGGCTCGTGCTTCTGGTTTTTTTCTGCACAGAAGGTACTTCTGGCAGCAATCGTTACGGAAACGATCCGAAGTTTGGTTCAAATTAATCTTAGAATTGGGGCGat                                                                                                                                             >  2:2807845/1‑150 (MQ=255)
              cAGGTGCCTGGGCCTTGCTCTATTTTGTTCCGTTTATTGTCTGGCTCGTGCTTCTGGTTTTTTTCTGCACAGAAGGTACTTCTGGCAGCAATCGTTACGGAAACGATAAGAAGTTTGGTTCAAATTAATCTTAGAATTGGGGCGATAttt                                                                                                                                         <  2:2727258/150‑1 (MQ=255)
               aGGTGCCTGGGCCTTGCTCTTTTTTGTTCCGTTTATTGGCTGGCTCGTGCTTCTGGTTTTTTTCTGCACAGAAGGTACTTCTGGCAGCAATCGTTACGGAAACGATCCGAAGTTTGGTTCAAATTAATCTTAGAATTGGGGCGATATTTc                                                                                                                                        >  1:2979485/1‑150 (MQ=255)
                   gCCTGGGCCTTGCTCTTTTTTGTTCCGTTTATTGGCTGGCTCGTGCTTCTGGTTTTTTTCTGCACAGAAGGTACTTCTGGCAGCAATCGTTACGGAAACGATCCGAAGTTTGGTTCAAATTAATCTTAGAATTGGGGCGATATTTCGccc                                                                                                                                    <  1:3014242/150‑1 (MQ=255)
                      tGGGCCTTGCTCTTTTTTGTTCCGTTTATTGGCTGGCTCGTGCTTCTGGTTTTTTTCTGCACAGAAGGTACTTCTGGCAGCAATCGTTACGGAAACGATCCGAAGTTTGGTTCAAATTAATCTTAGAATTGGGGCGATATTTCGCCCCtt                                                                                                                                 <  2:4325172/150‑1 (MQ=255)
                      tGGGCCTTGCTCTTTTTTGTTCCGTTTATTGGCTGGCTCGTGCTTCTGGTTTTTTTCTGCACAGAAGGTACTTCTGGCAGCAATCGTTACGGAAACGATCCGAAGTTTGGTTCAAATTAATCTTAGAATTGGGGCGATATTTAGCCCCtt                                                                                                                                 >  2:3200934/1‑150 (MQ=255)
                       gggCCTTGCTCTTTTTTGTTCCGTTTATTGGCTGGCTCGTGCTTCTGGTTTTTTTCTGCACAGAAGGTACTTCTGGCAGCAATCGTTACGGAAACGATCCGAAGTTTGGTTCAAATTAATCTTAGAATTGGGGCGATATTTCGCCCCttt                                                                                                                                <  2:644945/150‑1 (MQ=255)
                        ggCCTTGCTCTTTTTTGTTCCGTTTATTGGCTGGCTCGTGCTTCTGGTTTTTTTCTGCACAGAAGGTACTTCTGGCAGCAATCGTTACGGAAACGATCCGAAGTTTGGTTCAAATTAATCTTAGAATTGGGGCGATATTTCGCCCCtttt                                                                                                                               <  1:4107643/150‑1 (MQ=255)
                         gCCTTGCTCTTTTTTGTTCCGTTTATTGGCTGGCTCGTGCTTCTGGTTTTTTTCTGCACAGAAGGTACTTCTGGCAGCAATCGTTACGGAAACGATCCGAAGTTTGGTTCAAATTAATCTTAGAATTGGGGCGATATTTCGCCCCttttt                                                                                                                              >  2:3674224/1‑150 (MQ=255)
                          ccTTGCTCTTTTTTGTTCCGTTTATTGGCTGGCTCGTGCTTCTGGTTTTTTTCTGCACAGAAGGTACTTCTGGCAGCAATCGTTACGGAAACGATCCGAAGTTTGGTTCAAATTAATCTTAGAATTGGGGCGATATTTCGCCCCTTTtta                                                                                                                             <  2:4146066/150‑1 (MQ=255)
                          ccTTGCTCTTTTTTGTTCCGTTTATTGGCTGGCTCGTGCTTCTGGTTTTTTTCTGCACAGAAGGTACTTCTGGCAGCAATCGTTACGGAAACGATCCGAAGTTTGGTTCAAATTAATCTTAGAATTGGGGCGATATTTCGCCCCTTTtta                                                                                                                             <  2:1155502/150‑1 (MQ=255)
                           cTTGCTCTTTTTTGTTCCGTTTATTGGCTGGCTCGTGCTTCTGGTTTTTTTCTGCACAGAAGGTACTTCTGGCAGCAATCGTTACGGAAACGATCCGAAGTTTGGTTCAAATTAATCTTAGAATTGGGGCGATATTTCGCCCCTTTttat                                                                                                                            <  2:2783418/150‑1 (MQ=255)
                               ctctTTTTTGTTCCGTTTATTGGCTGGCTCGTGCTTCTGGTTTTTTTCTGCACAGAAGGTACTTCTGGCAGCAATCGTTACGGAAACGATCCGAAGTTTGGTTCAAATTAATCTTAGAATTGGGGCGATATTTCGCCCCTTTTTATTAAc                                                                                                                        <  1:550230/150‑1 (MQ=255)
                                 cttgtgtGTTCCGTTTATTGGCTGGCTCGTGCTTCTTGTTTTTTTCTGCACAGAAGGTACTTCTGGCAGCAATCGTTACGGAAACGATCCGAAGTTTGGTTCAAATTAATCTTAGAATTGGGGCGATATTTCGCCCCTTTTTATTAACaa                                                                                                                      <  1:3852471/144‑1 (MQ=255)
                                 ctTTTTTGTTCCGTTTATTGGCTGGCTCGTGCTTCTGGTTTTTTTCTGCACAGAATGTACTTCTGTCAGCAATCGTTACGGAAACGATCCGAAGTTTGGTTCAAATTAATCTTAGAATTGGGGCGATATTTCGCCCCTTTTTATTAACaa                                                                                                                      <  2:3470621/150‑1 (MQ=255)
                                 ctTTTTTGTTCCGTTTATTGGCTGGCTCGTGCTTCTGGTTTTTTTCTGCACAGAAGGTACTTCTGGCAGCAATCGTTACGGAAACGATCCGAAGTTTGGTTCAAATTAATCTTAGAATTGGGGCGATATTTCGCCCCTTTTTATTAACaa                                                                                                                      <  1:3620868/150‑1 (MQ=255)
                                  ttttttGTTCCGTTTATTGGCTGGCTCGTGCTTCTGGTTTTTTTCTGCACAGAAGGTACTTCTGGCAGCAATCGTTACGGAAACGATCCGAAGTTTGGTTCAAATTAATCTTAGAATTGGGGCGATATTTCGCCCCTTTTTATTAACaat                                                                                                                     >  2:3594367/1‑150 (MQ=255)
                                    ttttGTTCCGTTTATTGGCTGGCTCGTGCTTCTGGTTTTTTTCTGCACAGAAGGTACTTCTGGCAGCAATCGTTACGGAAACGATCCGAAGTTTGGTTCAAATTAATCTTAGAATTGGGGCGATATTTCGCCCCTTTTTATTAACaataa                                                                                                                   >  2:1575405/1‑150 (MQ=255)
                                          tCCGTTTATTGGCTGGCTCGTGCTTCTGGTTTTTTTCTGCACAGAAGGTACTTCTGGCAGCAATCGTTACGGAAACGATCCGAAGTTTGGTTCAAATTAATCTTAGAATTGGGGCGATATTTCGCCCCTTTTTATTAACAATaataaata                                                                                                             <  1:4230189/150‑1 (MQ=255)
                                               ttATTGGCTGGCTCGTGCTTCTGGTTTTTTTCTGCACAGAAGGTACTTCTGGCAGCAATCGTTACGGAAACGATCCGAAGTTTGGTTCAAATTAATCTTAGAATTGGGGCGATATTTCGCCCCTTTTTATTAACAATAATAAATattatt                                                                                                        <  1:2642717/150‑1 (MQ=255)
                                                 aTTGGCTGGCTCGTGCTTCTGGTTTTTTTCTGCACAGAAGGTACTTCTGGCAGCAATCGTTACGGAAACGATCCGAAGTTTGGTTCAAATTAATCTTAGAATTGGGGCGATATTTCGCCCC‑TTTTATTAACAATAATAAATATTATTTTc                                                                                                     >  1:3352189/1‑150 (MQ=255)
                                                   tggctggcTCGTGCTTCTGGTTTTTTTCTGCACAGAAGGTACTTCTGGCAGCAATCGTTACGGAAACGATCCGAAGTTTGGTTCAAATTAATCTTAGAATTGGGGCGATATTTCGCCCCTTTTTATTAACAATAATAAATATTATTTTcc                                                                                                    <  2:1857538/150‑1 (MQ=255)
                                                   tggctggcTCGTGCTTCTGGTTTTTTTCTGCACAGAAGGTACTTCTGGCAGCAATCGTTACGGAAACGATCCGAAGTTTGGTTCAAATTAATCTTAGAATTGGGGCGATATTTCGCCCCTTTTTATTAACAATAATAAATATTATTTTcc                                                                                                    <  2:1907404/150‑1 (MQ=255)
                                                   tggctggcTCGTGCTTCTGGTTTTTTTCTGCACAGAAGGTACTTCTGGCAGCAATCGTTACGGAAACGATCCGAAGTTTGGTTCAAATTAATCTTAGAATTGGGGCGATATTTCGCCCCTTTTTATTAACAATAATAAATATTATTTTcc                                                                                                    >  2:3140265/1‑150 (MQ=255)
                                                   tggctggcTCGTGCTTCTGGTTTTTTTCTGCACAGAAGGTACTTCTGGCAGCAATCGTTACGGAAACGATCCGAAGTTTGGTTCAAATTAATCTTAGAATTGGGGCGATATTTCGCCCCTTTTTATTAACAATAATAAATATTATTTTcc                                                                                                    <  1:2558162/150‑1 (MQ=255)
                                                   tggctggcTCGTGCTTCTGGTTTTTTTCTGCACAGAAGGTACTTCTGGCAGCAATCGTTACGGAAACGATCCGAAGTTTGGTTCAAATTAATCTTAGAATTGGGGCGATATTTCGCCCCTTTTTATTAACAATAATAAATATTATTTTcc                                                                                                    <  1:2558144/150‑1 (MQ=255)
                                                   tggctggcTCGTGCTTCTGGTTTTTTTCTGCACAGAAGGTACTTCTGGCAGCAATCGTTACGGAAACGATCCGAAGTTTGGTTCAAATTAATCTTAGAATTGGGGCGATATTTCGCCCCTTTTTATTAACAATAATAAATATTATTTTcc                                                                                                    <  2:2051808/150‑1 (MQ=255)
                                                   tggctggcTCGTGCTTCTGGTTTTTTTCTGCACAGAAGGTACTTCTGGCAGCAATCGTTACGGAAACGATCCGAAGTTTGGTTCAAATTAATCTTAGAATTGGGGCGATATTTCGCCCCTTTTTATTAACAATAATAAATATTATTTTcc                                                                                                    >  2:4113150/1‑150 (MQ=255)
                                                       tggctCGTGCTTCTGGTTTTTTTCTGCACAGAAGGTACTTCTGGCAGCAATCGTTACGGAAACGATCCGAAGTTTGGTTCAAATTAATCTTAGAATTGGGGCGATATTTCGCCCCTTTTTATTAACAATAATAAATATTATTTTCCGTTa                                                                                                <  2:3683009/150‑1 (MQ=255)
                                                       tggctCGTGCTTCTGGTTTTTTTCTGCACAGAAGGTACTTCTGGCAGCAATCGTTACGGAAACGATCCGAAGTTTGGTTCAAATTAATCTTAGAATTGGGGCGATATTTCGCCCCTTTTTATTAACAATAATAAATATTATTTTCCGTTa                                                                                                <  2:468889/150‑1 (MQ=255)
                                                       tggctCGTGCTTCTGGTTTTTTTCTGCACAGAAGGTACTTCTGGCAGCAATCGTTACGGAAACGATCCGAAGTTTGGTTCAAATTAATCTTAGAATTGGGGCGATATTTCGCCCCTTTTTATTAACAATAATAAATATTATTTTCCGTTa                                                                                                >  2:509363/1‑150 (MQ=255)
                                                         gctCGTGCTTCTGGTTTTTTTCTGCACAGAAGGTACTTCTGGCAGCAATCGTTACGGAAACGATCCGAAGTTTGGTTCAAATTAATCTTAGAATTGGGGCGATATTTCGCCCCTTTTTATTAACAATAATAAATATTATTTTCCGTTaaa                                                                                              <  1:2375026/150‑1 (MQ=255)
                                                         gctCGTGCTTCTGGTTTTTTTCTGCACAGAAGGTACTTCTGGCAGCAATCGTTACGGAAACGATCCGAAGTTTGGTTCAAATTAATCTTAGAATTGGGGCGATATTTCGCCCCTTTTTATTAACAATAATAAATATTATTTTCCGTTaaa                                                                                              >  2:551157/1‑150 (MQ=255)
                                                         gctCGTGCTTCTGGTTTTTTTCTGCACAGAAGGTACTTCTGGCAGCAATCGTTACGGAAACGATCCGAAGTTTGGTTCAAATTAATCTTAGAAGTGGGGCGATATTTCGCCCCTTTTTATTAACAATAATAAATATTATTTTCCGTTaaa                                                                                              >  2:3732879/1‑150 (MQ=255)
                                                           tCGTGCTTCTGGTTTTTTTCTGCACAGAATGTACTTCTGGCAGCAATCGTTACGGAAACGATCCGAAGTTTGGTTCAAATTAATCTTAGAATTGGGGCGATATTTCGCCCCTTTTTATTAACAATAATAAATATTATTTTCCGTTaaaaa                                                                                            >  2:1702201/1‑150 (MQ=255)
                                                           tCGTGCTTCTGGTTTTTTTCTGCACAGAAGGTACTTCTGGCAGCAATCGTTACGGAAACGATCCGAAGTTTGGTTCAAATTAATCTTAGAATTGGGGCGATATTTCGCCCCTTTTTATTAACAATAATAAATATTATTTTCCGTTaaaaa                                                                                            <  1:2363327/150‑1 (MQ=255)
                                                                cTTCTGGTTTTTTTCTGCACAGAAGGTACTTCTGGCAGCAATCGTTACGGAAACGATCCGAAGTTTGGTTCAAATTAATCTTAGAATTGGGGCGATATTTCGCCCCTTTTTATTAACAATAATAAATATTATTTTCCGTTAAAAAGTTTg                                                                                       <  1:733984/150‑1 (MQ=255)
                                                                   cTGGTTTTTTTCTGCACAGAAGGTACTTCTGGCAGCAATCGTTACGGAAACGATCCGAAGTTTGGTTCAAATTAATCTTAGAATTGGGGCGATATTTCGCCCCTTTTTATTAACAATAATAAATATTATTTTCCGTTAAAAAGTTTGGGa                                                                                    <  1:3385516/150‑1 (MQ=255)
                                                                     ggTTTTTTTCTGCACAGAAGGTACTTCTGGCAGCAATCGTTACGGAAACGATCCGAAGTTTGGTTCAAATTAATCTTAGAATTGGGGCGATATTTCGCCCCTTTTTATTAACAATAATAAATATTATTTTCCGTTAAAAAGTTTGGGAAt                                                                                  <  1:1282466/150‑1 (MQ=255)
                                                                     ggTTTTTTTCTGCACAGAAGGTACTTCTGGCAGCAATCGTTACGGAAACGATCCGAAGTTTGGTTCAAATTAATCTTAGAATTGGGGCGATATTTCGCCCCTTTTTATTAACAATAATAAATATTATTTTCCGTTAAAAAGTTTGGGAAt                                                                                  >  2:3109441/1‑150 (MQ=255)
                                                                     ggTTTTTTTCTGCACAGAAGGTACTTCTGGCAGCAATCGTTACGGAAACGATCCGAAGTTTGGTTCAAATTAATCTTAGAATTGGGGCGATATTTCGCCCCTTTTTATTAACAATAATAAATATTATTTTCCGTTAAAAAGTTTGGGAAt                                                                                  <  1:550579/150‑1 (MQ=255)
                                                                      gtatttttCTGCACAGAAGGTACTTCTGGCAGCAATCGTTACGGAAACGATCCGAAGTTTGGTTCAAATTAATCTTAGAATTGGGGCGATATTTCGCCCCTTTTTATTAACAATAATAAATATTATTTTCCGTTAAAAAGTTTGGGAAtt                                                                                 <  2:2104142/147‑1 (MQ=255)
                                                                      gTTTTTTTCTGCACAGAAGGTACTTCTGGCAGCAATCGTTACGGAAACGATCCGAAGTTTGGTTCAAATTAATCTTAGAATTGGGGCGATATTTCGCCCCTTTTTATTAACAATAATAAATATTATTTTCCGTTAAAAAGTTTGGGAAtt                                                                                 <  1:346823/150‑1 (MQ=255)
                                                                      gTTTTTTTCTGCACAGAAGGTACTTCTGGCAGCAATCGTTACGGAAACGATCCGAAGTTTGGTTCAAATTAATCTTAGAATTGGGGCGATATTTCGCCCCTTTTTATTAACAATAATAAATATTATTTTCCGTTAAAAAGTTTGGGAAtt                                                                                 <  2:3015319/150‑1 (MQ=255)
                                                                        ttttttCTGCACAGAAGGTACTTCTGGCAGCAATCGTTACGGAAACGATCCGAAGTTTGGTTCAAATTAATCTTAGAATTGGGGCGATATTTCGCCCCTTTTTATTAACAATAATAAATATTATTTTCCGTTAAAAAGTTTGGGAATTTc                                                                               <  2:1552824/150‑1 (MQ=255)
                                                                             tCTGCACAGAAGGTACTTCTGGCAGCAATCGTTACGGAAACGATCCGAAGTTTGGTTCAAATTAATCTTAGAATTGGGGCGATATTTCGCCCCTTTTTATTAACAATAATAAATATTATTTTCCGTTAAAAAGTTTGGGAATTTCCCGCa                                                                          <  1:780293/150‑1 (MQ=255)
                                                                              cTGCACAGAAGGTACTTCTGGCAGCAATCGTTACGGAAACGATCCGAAGTTTGGTTCAAATTAATCTTAGAATTGGGGCGATATTTCGCCCCTTTTTATTAACAATAATAAATATTATTTTCCGTTAAAATGTTTGGGAATTTCGCGCAg                                                                         >  1:2965490/1‑150 (MQ=255)
                                                                              cTGCACAGAAGGTACTTCTGGCAGCAATCGTTACGGAAACGATCCGAAGTTTGGTTCAAATTAATCTTAGAATTGGGGCGATATTTCGCCCCTTTTTATTAACAATAATAAATATTATTTTCCGTTAAAAAGTTTGGGAATTTCCCGCAg                                                                         >  1:4430124/1‑150 (MQ=255)
                                                                                 cacaGAAGGTACTTCTGGCAGCAATCGTTACGGAAACGATCCGAAGTTTGGTTCAAATTAATCTTAGATTTGGGGCGATATTTCGCCCCTTTTTATTAACAATAATAAATATTATTTTCCGTTAAAAAGTTTGGGAATTTCCCGCAGaca                                                                      <  1:1549917/150‑1 (MQ=255)
                                                                                  acaGAAGGTACTTCTGGCAGCAATCGTTACGGAAACGATCCGAAGTTTGGTTCAAATTAATCTTAGAATTGGGGCGATATTTCGCCCCTTTTTATTAACAATAATAAATATTATTTTCCGTTAAAAAGTTTGGGAATTTCCCGCAGacac                                                                     >  2:1250905/1‑150 (MQ=255)
                                                                                   caGAAGGTACTTCTGGCAGCAATCGTTACGGAAACGATCCGAAGTTTGGTTCAAATTAATCTTAGAATTGGGGCGATATTTCGCCCCTTTTTATTAACAATAATAATTATTATTTTCCGTTCAAAAGTTTGGGAATTTCCCGCAGACAcc                                                                    >  2:390664/1‑150 (MQ=255)
                                                                                   caGAAGGTACTTCTGGCAGCAATCGTTACGGAAACGATCCGAAGTTTGGTTCAAATTAATCTTAGAATTGGGGCGATATTTCGCCCCTTTTTATTAACAATAATAAATATTATTTTCCGTTAAAAAGTTTGGGAATTTCCCGCAGACAcc                                                                    >  2:878044/1‑150 (MQ=255)
                                                                                        ggTACTTCTGGCAGCAATCGTTACGGAAACGATCCGAAGTTTGGTTCAAATTAATCTTAGAATTGGGGCGATATTTCGCCCCTTTTTATTAACAATAATAAATATTATTTTCCGTTAAAAAGTTTGGGAATTTCCCGCAGACACCAGGAt                                                               <  1:617224/150‑1 (MQ=255)
                                                                                         gTACTTCTGGCAGCAATCGTTACGGAAACGATCCGAAGTTTGGTTCAAATTAATCTTAGAATTGGGGCGATATTTCGCCCCTTTTTATTAACAATAATAAATATTATTTTCCGTTAAAAAGTTTGGGAATTTCCCGCAGACACCAGGAtt                                                              >  2:2436703/1‑150 (MQ=255)
                                                                                                   cagcaATCCTTACGGAAACGATCCGAAGTTTGTTTCAAATTAATCTTATAATTGGGGCGATATATCGACCCATTTTATAAACAATAATAAATAAAATTTACCGTTAAAAAGTATGTTAATTTACCGAATACAACAGGATTTAGATTaccc                                                    >  1:4286705/1‑150 (MQ=255)
                                                                                                    agcaATCGTTACGGAAACGATCCGAAGTTTGGTTCAAATTAATCTTAGAATTGGGGCGATATTTCGCCCCTTTTTATTAACAATAATAAATATTATTTTCCGTTAAAAAGTTTGGGAATTTCCCGCAGACACCAGGATTTCGCTTccccc                                                   >  1:1567116/1‑150 (MQ=255)
                                                                                                    agcaATCGTTACGGAAACGATCCGAAGTTTGGTTCAAATTAATCTTAGAATTGGGGCGATATTTCGCCCCTTTTTATTAACAATAATAAATATTATTTTCCGTTAAAAAGTTTGGGAATTTCCCGCAGACACCAGGATTTCGCTTccccc                                                   <  1:1808981/150‑1 (MQ=255)
                                                                                                       aaTCGTTACGGAAACGATCCGAAGTTTGGTTCAAATTAATCTTAGATTTGGGGCGATATTTCGCCCCTTTTTATTAACAATAATAAATATTATTTTCCGTTAAAAAGTTTGGGAATTTCCCGCAGACACCAGGATTTCGCTTCCCCCATa                                                <  2:1656198/150‑1 (MQ=255)
                                                                                                       aaTCGTTACGGAAACGATCCGAAGTTTGGTTCAAATTAATCTTAGAATTGGGGCGATATTTCGCCCCTTTTTATTAACAATAATAAATATTATTTTCCGTTAAAAAGTTTGGGAATTTCCCGCAGACACCAGGATTTCGCTTCCCCCATa                                                >  1:3735143/1‑150 (MQ=255)
                                                                                                          cGTTACGGAAACGATCCGAAGTTTGGTTCAAATTAATCTTAGAATTGGGGCGATATTTCGCCCCTTTTTATTAACAATAATAAATATTATTTTCCGTTAAAAAGTTTGGGAATTTCCCGCAGACACCAGGATTTCGCTTCCCCCATACTg                                             >  1:3199959/1‑150 (MQ=255)
                                                                                                           gTTACGGAAACGATCCGAAGTTTGGTTCAAATTAATCTTAGAATTGGGGCGATATTTCGCCCCTTTTTATTAACAATAATAAATATTATTTTCCGTTAAAAAGTTTGGGAATTTCCCGCAGACACCAGGATTTCGCTTCCCCCATACTGt                                            >  2:4213761/1‑150 (MQ=255)
                                                                                                           gTTACGGAAACGATCCGAAGTTTGGTTCAAATTAATCTTAGAATTGGGGCGATATTTCGCCCCTTTTTATTAACAATAATAAATATTATTTTCCGTTAAAAAGTTTGGGAATTTCCCGCAGACACCAGGATTTCGCTTCCCCCATACTGt                                            >  1:2638199/1‑150 (MQ=255)
                                                                                                               cGGAAACGATCCGAAGTTTGGTTCAAATTAATCTTAGAATTGGGGCGATATTTCGCCCCTTTTTATTAACAATAATAAATATTATTTTCCGTTAAAAAGTTTGGGAATTTCCCGCAGACACCAGGATTTCGCTTCCCCCATACTGTCacg                                        <  1:3646621/150‑1 (MQ=255)
                                                                                                                  aaaCGATCCGAAGTTTGGTTCAAATTAATCTTAGAATTGGGGCGATATTTCGCCCCTTTTTATTAACAATAATAAATATTATTTTCCGTTAAAAAGTTTGGGAATTTCCCGCAGACACCAGGATTTCGCTTCCCCCATACTGTCacgacg                                     >  1:1084717/1‑150 (MQ=255)
                                                                                                                     cGATCCGAAGTTTGGTTCAAATTAATCTTAGAATTGGGGCGATATTTCGCCCCTTTTTATTAACAATAATAAATATTATTTTCCGTTAAAAAGTTTGGGAATTTCCCGCAGACACCAGGATTTCGCTTCCCCCATACTGTCACGACGCCa                                  >  1:1634142/1‑150 (MQ=255)
                                                                                                                     cGATCCGAAGTTTGGTTCAAATTAATCTTAGAATTGGGGCGATATTTCGCCCCTTTTTATTAACAATAATAAATATTATTTTCCGTTAAAAAGTTTGGGAATTTCCCGCAGACACCAGGATTTCGCTTCCCCCATACTGTCACGACGCCa                                  >  1:3911570/1‑150 (MQ=255)
                                                                                                                       aTCCGAAGTTTGGTTCAAATTAATCTTAGAATTGGGGCGATATTTCGCCCCTTTTTATTAACAATAATAAATATTATTTTCCGTTAAAAAGTTTGGGAATTTCCCGCAGACACCAGGATTTCGCTTCCCCCATACTGTCACGACGCCAgg                                >  2:4497156/1‑150 (MQ=255)
                                                                                                                        tCCGAAGTTTGGTTCAAATTAATCTTAGAATTGGGGCGATATTTCGCCCCTTTTTATTAACAATAATAAATATTATTTTCCGTTAAAAAGTTTGGGAATTTCCCGCAGACACCAGGATTTCGCTTCCCCCATACTGTCACGACGCCAGGc                               <  1:1759586/150‑1 (MQ=255)
                                                                                                                          cGAAGTTTGGTTCAAATTAATCTTAGAATTGGGGCGATATTTCGCCCCTTTTTATTAACAATAATAAATATTATTTTCCGTTAAAAAGTTTGGGAATTTCCCGCAGACACCAGGATTTCGCTTCCCCCATACTGTCACGACGCCAGGCCa                             >  2:90667/1‑150 (MQ=255)
                                                                                                                            aaGTTTGGTTCAAATTAATCTTAGAATTGGGGCGATATTTCGCCCCTTTTTATTAACAATAATAAATATTATTTTCCGTTAAAAAGTTTGGGAATTTCCCGCAGACACCAGGATTTCGCTTCCCCCATACTGTCACGACGCCAGGCCata                           >  1:281450/1‑150 (MQ=255)
                                                                                                                              gTTTGGTTCAAATTAATCTTAGAATTGGGGCGATATTTCGCCCCTTTTTATTAACAATAATAAATATTATTTTCCGTTAAAAAGTTTGGGAATTTCCCGCAGACACCAGGATTTCGCTTCCCCCATACTGTCACGACGCCAGGCCataat                         <  1:2708960/150‑1 (MQ=255)
                                                                                                                              gTTTGGTTCAAATTAATCTTAGAATTGGGGCGATATTTCGCCCCTTTTTATTAACAATAATAAATATTATTTTCCGTTAAAAAGTTTGGGAATTTCCCGCAGACACCAGGATTTCGCTTCCCCCATACTGTCACGACGCCAGGCCataat                         >  1:4517344/1‑150 (MQ=255)
                                                                                                                                 tGGTTCAAATTAATCTTAGAATTGGGGCGATATTTCGCCCCTTTTTATTAACAATAATAAATATTATTTTCCGTTAAAAAGTTTGGGAATTTCCCGCAGACACCAGGATTTCGCTTCCCCCATACTGTCACGACGCCAGGCCataataat                      >  2:3010227/1‑150 (MQ=255)
                                                                                                                                  ggTTCAAATTAATCTTAGAATTGGGGCGATATTTCGCCCCTTTTTATTAACAATAATAAATATTATTTTCCGTTAAAAAGTTTGGGAATTTCCCGCAGACACCAGGATTTCGCTTCCCCCATACTGTCACGACGCCAGGCCataataata                     >  2:2552817/1‑150 (MQ=255)
                                                                                                                                     tCAAATTAATCTTAGAATTGGGGCGATATTTCGCCCCTTTTTATTAACAATAATAAATATTATTTTCCGTTAAAAAGTTTGGGAATTTCCCGCAGACACCAGGATTTCGCTTCCCCCATACTGTCACGACGCCAGGCCATAATAATATCg                  <  1:1347746/150‑1 (MQ=255)
                                                                                                                                        aaTTAATCTTAGATTTGGGGCGATATTTCGCCCCTTTTTATTAACAATAATAAATATTATTTTCCGTTAAAAAGTTTGGGAATTTCCCGCAGACACCAGGATTTCGCTTCCCCCATACTGTCACGACGCCAGGCCATAATAATATCGAtc               <  1:2327795/150‑1 (MQ=255)
                                                                                                                                        aaTTAATCTTAGAATTGGGGCGATATTTCGCCCCTTTTTATTAACAATAATAAATATTATTTTCCGTTAAAAAGTTTGGGAATTTCCCGCAGACACCAGGATTTCGCTTCCCCCATACTGTCACGACGCCAGGCCATAATAATATCGAtc               <  1:2440148/150‑1 (MQ=255)
                                                                                                                                         aTTAATCTTAGAATTGGGGCGATATTTCGCCCCTTTTTATTAACAATAATAAATATTATTTTCCGTTAAAAAGTTTGGGAATTTCCCGCAGACACCAGGATTTCGCTTCCCCCATACTGTCACGACGCCAGGCCATAATAATATCGAtct              <  1:3903289/150‑1 (MQ=255)
                                                                                                                                             aTCTTAGAATTGGGGCGATATTTCGCCCCTTTTTATTAACAATAATAAATATTATTTTCCGTTAAAAAGTTTGGGAATTTCCCGCAGACACCAGGATTTCGCTTCCCCCATACTGTCACGACGCCAGGCCATAATAATATCGATCTCGCt          <  2:3303017/150‑1 (MQ=255)
                                                                                                                                               cTTAGAATTGGGGCGATATTTCGCCCCTTTTTATTAACAATAATAAATATTATTTTCCGTTAAAAAGTTTGGGAATTTCCCGCAGACACCAGGATTTCGCTTCCCCCATACTGTCACGACGCCAGGCCCTAATAATATCGATCTCGCTgg        >  1:913823/1‑150 (MQ=255)
                                                                                                                                                ttAGATTTGGGGCGATATTTCGCCCCTTTTTATTAACAATAATAAATATTATTTTCCGTTAAAAAGTTTGGGAATTTCCCGCAGACACCAGGATTTCGCTTCCCCCATACTGTCACGACGCCAGGCCATAATAATATCGATCTCGCTGGt       >  2:3285811/1‑150 (MQ=255)
                                                                                                                                                ttAGAATTGGGGCGATATTTCGCCCCTTTTTATTAACAATAATAAATATTATTTTCCGTTAAAAAGTTTGGGAATTTCCCGCAGACACCAGGATTTCGCTTCCCCCATACTGTCACGACGCCAGGCCATAATAATATCGATCTCGCTGGt       <  1:3502601/150‑1 (MQ=255)
                                                                                                                                                ttAGAATTGGGGCGATATTTCGCCCCTTTTTATTAACAATAATAAATATTATTTTCCGTTAAAAAGTTTGGGAATTTCCCGCAGACACCAGGATTTCGCTTCCCCCATACTGTCACGACGCCAGGCCATAATAATATCGATCTCGCTGGt       <  1:3502355/150‑1 (MQ=255)
                                                                                                                                                 tAGAATTGGGGCGATATTTCGCCCCTTTTTATTAACAATAATAAATATTATTTTCCGTTAAAAAGTTTGGGAATTTCCCGCAGACACCAGGATTTCGCTTCCCCCATACTGTCACGACGCCAGGCCATAATAATATCGATCTCGCTGGTc      <  2:1355265/150‑1 (MQ=255)
                                                                                                                                                 tAGAATTGGGGCGATATTTCGCCCCTATTTATTAACAATAATAAATATTATTTTCCGTTAAAAAGTTTGGGAATTTCCCGCAGACACCAGGATTTCGCTTCCCCCATACTGTCACGACGCCAGGCCATAATAATATCGATCTCGCTGGTc      <  2:682548/150‑1 (MQ=255)
                                                                                                                                                  aGAATTGGGGCGATATTTCGCCCCTTTTTATTAACAATAATAAATATTATTTTCCGTTAAAAAGTTTGGGAATTTCCCGCAGACACCAGGATTTCGCTTCCCCCATACTGTCACGACGCCAGGCCATCATAATATCGATCTCGCTGGTCg     >  2:3856211/1‑150 (MQ=255)
                                                                                                                                                  aGAATTGGGGCGATATTTCGCCCCTTTTTATTAACAATAATAAATATTATTTTCCGTTAAAAAGTTTGGGAATTTCCCGCAGACACCAGGATTTCGCTTCCCCCATACTGTCACGACGCCAGGCCATAATAATATCGATCTCGCTGGTCg     >  2:3349573/1‑150 (MQ=255)
                                                                                                                                                  aGAATTGGGGCGATATTTCGCCCCTTTTTATTAACAATAATAAATATTATTTTCCGTTAAAAAGTTTGGGAATTTCCCGCAGACACCAGGATTTCGCTTCCCCCATACTGTCACGACGCCAGGCCATAATAATATCGATCTCGCTGGTCg     >  2:1795923/1‑150 (MQ=255)
                                                                                                                                                    aaTTGGGGCGATATTTCGCCCCTTTTTATTAACAATAATAAATATTATTTTCCGTTAAAAAGTTTGGGAATTTCCCGCAGACACCAGGATTTCGCTTCCCCCATACTGTCACGACGCCAGGCCATAATAATATCGATCTCGCTGGTCGAt   >  1:1609230/1‑150 (MQ=255)
                                                                                                                                                     aTTGGGGCGATATTTCGCCCCTTTTTATTAACAATAATAAATATTATTTTCCGTTAAAAAGTTTGGGAATTTCCCGCAGACACCAGGATTTCGCTTCCCCCATACTGTCACGACGCCAGGCCATAATAATATCGATCTCGCTGGTCGAtt  >  1:721592/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
CGATACCGACCGTTCAGGTGCCTGGGCCTTGCTCTTTTTTGTTCCGTTTATTGGCTGGCTCGTGCTTCTGGTTTTTTTCTGCACAGAAGGTACTTCTGGCAGCAATCGTTACGGAAACGATCCGAAGTTTGGTTCAAATTAATCTTAGAATTGGGGCGATATTTCGCCCCTTTTTATTAACAATAATAAATATTATTTTCCGTTAAAAAGTTTGGGAATTTCCCGCAGACACCAGGATTTCGCTTCCCCCATACTGTCACGACGCCAGGCCATAATAATATCGATCTCGCTGGTCGATT  >  CP009273/3246485‑3246783

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A