Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F78 I0 R1 626 241.3 10389380 77.7% 8072548 149.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 411,027 G→T 94.0% L61L (CTC→CTA)  sbcC ← exonuclease, dsDNA, ATP‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273411,0270GT94.0% 802.8 / 33.2 244L61L (CTC→CTA) sbcCexonuclease, dsDNA, ATP‑dependent
Reads supporting (aligned to +/- strand):  ref base G (5/10);  new base T (98/131);  total (103/141)
Fisher's exact test for biased strand distribution p-value = 5.94e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 248 total aligned reads displayed.

TGCAAATTACCGTCGGGTTGGTTACGCGCCCGATTCTGGCTCCAGAATGCACGGTACGCTTCACCTTTCACTTCAAACTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGAGAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACAGCCCGTTGCTGGCGAACGGCTCGCGGGTGAAATCAATCTTCCATTCGCCTTTTAATG  >  CP009273/410881‑411172
                                                                                                                                                  |                                                                                                                                                 
tGCAAATTACCGTCGGGTTGGTTACGCGCCCGATTCTGGCTCCAGAATGCACGGTACGCTTCACCTTTCACTTCAAACTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTtagataga                                                                                                                                                <  1:87989/150‑1 (MQ=255)
  cAAATTACCGTCGGGTTGGTTACGCGCCCGATTCTGGCTCCAGAATGCACGGTACGCTTCACCTTTCACTTCAAACTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGAcg                                                                                                                                              >  2:2011808/1‑150 (MQ=255)
    aaTTACCGTCGGGTTGGTTACGCGCCCGATTCTGGCTCCAGAATGCACGGTACGCTTCACCTTTCACTTCAAACTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCCTTTTGCGATTGTGAAACGTTAGATAGAcgcg                                                                                                                                            >  2:3450944/1‑150 (MQ=255)
    aaTTACCGTCGGGTTGGTTACGCGCCCGATTCTGGCTCCAGAATGCACGGTACGCTTCACCTTTCACTTCAAACTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGAcgcg                                                                                                                                            <  1:808433/150‑1 (MQ=255)
    aaTGACCGTCGGGTTGGTTACGCGCCCGATTCTGGCTCCAGAATGCACGGTACGCTTCACCTTTCACTTCAAACTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGAcgcg                                                                                                                                            <  1:358117/150‑1 (MQ=255)
      ttACCGTCGGGTTGGTTACGCGCCCGATTCTGGCTCCAGAATGCACGGTACGCTTCACCTTTCACTTCAAACTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGa                                                                                                                                          <  2:1690185/150‑1 (MQ=255)
      ttACCGTCGGGTTGGTTACGCGCCCGATTCTGGCTCCAGAATGCACGGTACGCTTCACCTTTCACTTCAAACTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGa                                                                                                                                          <  2:2315917/150‑1 (MQ=255)
       tACCGTCGGGTTGGTTACGCGCCCGATTCTGGCTCCAGAATGCACGGTACGCTTCACCTTTCACTTCAAACTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAg                                                                                                                                         <  1:1094204/150‑1 (MQ=255)
       tACCGTCGGGTTGGTTACGCGCCCGATTCTGGCTCCAGAATGCACGGTACGCTTCACCTTTCACTTCAAACTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAg                                                                                                                                         >  1:596652/1‑150 (MQ=255)
           gTCGGGTTGGTTACGCGCCCGATTCTGGCTCCAGAATGCACGGTACGCTTCACCTTTCACTTCAAACTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTc                                                                                                                                     <  1:695496/150‑1 (MQ=255)
           gTCGGGTTGGTTACGCGCCCGATTCTGGCTCCAGAATGCACGGTACGCTTCACCTTTCACTTCAAACTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTc                                                                                                                                     <  2:4710512/150‑1 (MQ=255)
              gggTTGGTTACGCGCCCGATTCTGGCTCCAGAATGCACGGTACGCTTCACCTTTCACTTCAAACTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTg                                                                                                                                  >  1:681117/1‑150 (MQ=255)
                  tggttACGCGCCCGATTCTGGCTCCAGAATGCACGGTACGCTTCACCTTTCACTTCAAACTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATAc                                                                                                                              >  2:4916525/1‑150 (MQ=255)
                  tggttACGCGCCCGATTCTGGCTCCAGAATGCACGGTACGCTTCACCTTTCACTTCAAACTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATAc                                                                                                                              <  2:4535026/150‑1 (MQ=255)
                   ggttACGCGCCCGATTCTGGCTCCAGAATGCACGGTACGCTTCACCTTTCACTTCAAACTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACa                                                                                                                             <  1:996484/150‑1 (MQ=255)
                         gcgcCCGATTCTGGCTCCAGAATGCACGGTACGCTTCACCTTTCACTTCAAACTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCa                                                                                                                       <  2:4273991/150‑1 (MQ=255)
                          cgcCCGATTCTGGCTCCAGAATGCACGGTACGCTTCACCTTTCACTTCAAACTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAg                                                                                                                      <  2:4963573/150‑1 (MQ=255)
                          cgcCCGATTCTGGCTCCAGAATGCACGGTACGCTTCACCTTTCACTTCAAACTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAg                                                                                                                      >  1:3132929/1‑150 (MQ=255)
                          cgcCCGATTCTGGCTCCAGAATGCACGGTACGCTTCACCTTTCACTTCAAACTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAg                                                                                                                      >  1:1277314/1‑150 (MQ=255)
                                aTTCTGGCTCCAGAATGCACGGTACGCTTCACCTTTCACTTCAAACTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAAt                                                                                                                <  2:675338/150‑1 (MQ=255)
                                aTTCTGGCTCCAGAATGCACGGTACGCTTCACCTTTCACTTCAAACTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAAt                                                                                                                <  1:3907493/150‑1 (MQ=255)
                                aTTCTGGCTCCAGAATGCACGGTACGCTTCACCTTTCACTTCAAACTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAAt                                                                                                                <  1:2702649/150‑1 (MQ=255)
                                     ggCTCCAGAATGCACGGTACGCTTCACCTTTCACTTCAAACTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGt                                                                                                           <  2:4451778/150‑1 (MQ=255)
                                      gCTCCAGAATGCACGGTACGCTTCACCTTTCACTTCAAACTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTc                                                                                                          <  1:1051079/150‑1 (MQ=255)
                                       cTCCAGAATGCACGGTACGCTTCACCTTTCACTTCAAACTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTcc                                                                                                         <  1:961967/150‑1 (MQ=255)
                                        tCCAGAATGCACGGTACGCTTCACCTTTCACTTCAAACTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCca                                                                                                        <  1:1508266/150‑1 (MQ=255)
                                         ccAGAATGCACGGTACGCTTCACCTTTCACTTCAAACTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCcag                                                                                                       <  2:3126060/150‑1 (MQ=255)
                                          cAGAATGCACGGTACGCTTCACCTTTCACTTCAAACTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCcagc                                                                                                      >  1:3238782/1‑150 (MQ=255)
                                             aaTGCACGGTACGCTTCACCTTTCACTTCAAACTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAgg                                                                                                   <  1:5181742/150‑1 (MQ=255)
                                              aTGCACGGTACGCTTCACCTTTCACTTCAAACTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAggg                                                                                                  >  2:1266787/1‑150 (MQ=255)
                                                 cACGGTACGCTTCACCTTTCACTTCAAACTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATATACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGggtgg                                                                                               <  2:508994/150‑1 (MQ=255)
                                                  aCGGTACGCTTCACCTTTCACTTCAAACTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGAGAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGggtggt                                                                                              <  1:3271353/150‑1 (MQ=255)
                                                   cGGTACGCTTCACCTTTCACTTCAAACTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGtt                                                                                             <  2:1727863/150‑1 (MQ=255)
                                                   cGGTACGCTTCACCTTTCACTTCAAACTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGAGAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGtt                                                                                             <  1:2112287/150‑1 (MQ=255)
                                                   cGGTACGCTTCACCTTTCACTTCAAACTCCACCTCCGCCAGACAATCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGAGAGACGCGGAGGTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGAGGtt                                                                                             >  2:2420843/1‑150 (MQ=255)
                                                     gTACGCTTCACCTTTCACTTCAAACTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGtttt                                                                                           <  2:367315/150‑1 (MQ=255)
                                                     gTACGCTTCACCTTTCACTTCAAACTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGtttt                                                                                           >  1:2794857/1‑150 (MQ=255)
                                                     gTACGCTTCACCTTTCACTTCAAACTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGtttt                                                                                           <  2:4799395/150‑1 (MQ=255)
                                                         gCTTCACCTTTCACTTCAAACTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAATTGGCGTCCAGCTGGGTGGTTTTcccc                                                                                       <  1:5132375/150‑1 (MQ=255)
                                                          cTTCACCTTTCACTTCAAACTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCg                                                                                      >  2:970000/1‑150 (MQ=255)
                                                            tCACCTTTCACTTCAAACTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCa                                                                                    >  1:2187423/1‑150 (MQ=255)
                                                               ccTTTCACTTCAAACTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGCCAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCt                                                                                 <  2:1765523/150‑1 (MQ=255)
                                                               ccTTTCACTTCAAACTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCt                                                                                 <  2:2612589/150‑1 (MQ=255)
                                                               ccTTTCACTTCAAACTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCt                                                                                 <  2:3580151/150‑1 (MQ=255)
                                                                cTTTCACTTCAAACTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTg                                                                                >  2:3515622/1‑150 (MQ=255)
                                                                  ttCACTTCAAACTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGtt                                                                              <  1:1366447/150‑1 (MQ=255)
                                                                  ttCACTTCAAACTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGtt                                                                              <  2:3555543/150‑1 (MQ=255)
                                                                   tCACTTCAAACTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTg                                                                             <  2:1076113/150‑1 (MQ=255)
                                                                   tCACTTCAAACTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTg                                                                             >  1:375999/1‑150 (MQ=255)
                                                                      cTTCAAACTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGc                                                                          >  1:5087653/1‑150 (MQ=255)
                                                                       ttCAAACTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGcc                                                                         <  2:1574148/150‑1 (MQ=255)
                                                                           aaCTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTa                                                                     >  1:1148309/1‑150 (MQ=255)
                                                                              tCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATa                                                                  <  2:2524366/150‑1 (MQ=255)
                                                                                cACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGc                                                                >  2:305860/1‑150 (MQ=255)
                                                                                cACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGc                                                                <  1:4928518/150‑1 (MQ=255)
                                                                                cACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGc                                                                >  2:2539528/1‑150 (MQ=255)
                                                                                 aCCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCa                                                               <  2:2133246/150‑1 (MQ=255)
                                                                                  ccTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCaa                                                              <  1:710066/150‑1 (MQ=255)
                                                                                   cTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGAGAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCaaa                                                             >  1:4294825/1‑150 (MQ=255)
                                                                                    tCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAAc                                                            <  1:4610925/150‑1 (MQ=255)
                                                                                    tCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAAc                                                            <  1:2424494/150‑1 (MQ=255)
                                                                                    tCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGAGAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAAc                                                            <  2:4506760/150‑1 (MQ=255)
                                                                                     ccGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACa                                                           <  2:333771/150‑1 (MQ=255)
                                                                                      cGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACAg                                                          >  2:2288610/1‑150 (MQ=255)
                                                                                       gCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACAGc                                                         >  2:510354/1‑150 (MQ=255)
                                                                                       gCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACAGc                                                         >  1:2503598/1‑150 (MQ=255)
                                                                                        ccAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGAGAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACAGcc                                                        <  1:5030638/150‑1 (MQ=255)
                                                                                              aTTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACAGCCCGTTGc                                                  >  2:1402281/1‑150 (MQ=255)
                                                                                               ttCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACAGCCCGTTGCt                                                 <  1:4421180/150‑1 (MQ=255)
                                                                                               ttCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACAGCCCGTTGCt                                                 <  1:183176/150‑1 (MQ=255)
                                                                                               ttCGGCGGTATCGCGGGTCATGAGATCATTGTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACAGCCCGTTGCt                                                 <  2:4030464/150‑1 (MQ=255)
                                                                                                tCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACAGCCCGTTGCTg                                                <  2:2841294/150‑1 (MQ=255)
                                                                                                 cggcggTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACAGCCCGTTGCTgg                                               <  1:3647870/150‑1 (MQ=255)
                                                                                                 cggcggTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACAGCCCGTTGCTgg                                               <  2:3925987/150‑1 (MQ=255)
                                                                                                 cggcggTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACAGCCCGTTGCTgg                                               >  1:3739113/1‑150 (MQ=255)
                                                                                                  ggcggTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACAGCCCGTTGCTGGc                                              >  1:2596634/1‑150 (MQ=255)
                                                                                                     ggTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACAGCCCGTTGCTGGCGaa                                           >  2:4693839/1‑150 (MQ=255)
                                                                                                      gTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACAGCCCGTTGCTGGCGAAc                                          >  2:778138/1‑150 (MQ=255)
                                                                                                         tCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACAGCCCGTTGCTGGCGAACGGc                                       >  2:3977540/1‑150 (MQ=255)
                                                                                                           gcgGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACAGCCCGTTGCTGGCGAACGGCTc                                     >  1:78270/1‑150 (MQ=255)
                                                                                                            cgGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACAGCCCGTTGCTGGCGAACGGCTcg                                    >  1:4210565/1‑150 (MQ=255)
                                                                                                               gTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACAGCCCGTTGCTGGCGAACGGCTCGCgg                                 <  2:3768378/150‑1 (MQ=255)
                                                                                                               gTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACAGCCCGTTGCTGGCGAACGGCTCGCgg                                 >  2:918328/1‑150 (MQ=255)
                                                                                                                   tGAGATCATTTTGCGATTGTGAAACGTTAGAGAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACAGCCCGTTGCTGGCGAACGGCTCGCGGGTGa                             <  2:3254463/150‑1 (MQ=255)
                                                                                                                      gaTCATTGTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACAGCCCGTTGCTGGCGAACGGCTCGCGGGTGAaat                          <  2:3241479/150‑1 (MQ=255)
                                                                                                                         cATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACAGCCCGTTGCTGGCGAACGGCTCGCGGGTGAaatcaa                       >  1:1460927/1‑150 (MQ=255)
                                                                                                                         cATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGATGGGCCGGTAATAGCAAACAGCCCGTTGCTGGCGAACGGCTCGCGGGTGAaatcaa                       >  2:4531404/1‑150 (MQ=255)
                                                                                                                           ttttGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACAGCCCGTTGCTGGCGAACGGCTCGCGGGTGAaatcaatc                     <  1:4743256/150‑1 (MQ=255)
                                                                                                                             ttGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACAGCCCGTTGCTGGCGAACGGCTCGCGGGTGAAATCAATCtt                   <  2:1854168/150‑1 (MQ=255)
                                                                                                                              tGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACAGCCCGTTGCTGGCGAACGGCTCGCGGGTGAAATCAATCTTc                  >  2:886865/1‑150 (MQ=255)
                                                                                                                              tGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACAGCCCGTTGCTGGCGAACGGCTCGCGGGTGAAATCAATCTTc                  >  2:2412187/1‑150 (MQ=255)
                                                                                                                              tGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACAGCCCGTTGCTGGCGAACGGCTCGCGGGTGAAATCAATCTTc                  >  2:1221436/1‑150 (MQ=255)
                                                                                                                                 gATTGTGAAACGTTAGAGAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACAGCCCGTTGCTGGCGAACGGCTCGCGGGTGAAATCAATCTTCCAt               <  2:4036251/150‑1 (MQ=255)
                                                                                                                                      tgAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAACCAGCCCGTTGCTGGCGAACGGCTCGCGGGTGAAATCAATCTTCCATTCGcc          <  2:4896459/150‑1 (MQ=255)
                                                                                                                                      tgAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACAGCCCGTTGCTGGCGAACGGCTCGCGGGTGAAATCAATCTTCCATTCGcc          <  2:1592430/150‑1 (MQ=255)
                                                                                                                                       gAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACAGCCCGTTGCTGGCGAACGGCTCGCGGGTGAAATCAATCTTCCATTCGCCt         >  1:1594658/1‑150 (MQ=255)
                                                                                                                                           cGTTAGAGAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACAGCCCGTTGCTGGCGAACGGCTCGCGGGTGAAATCAATCTTCCATTCGCCTTTTa     <  1:4724127/150‑1 (MQ=255)
                                                                                                                                            gTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACAGCCCGTTGCTGGCGAACGGCTCGCGGGTGAAATCAATCTTCCATTCGCCTTTTaa    >  1:1718169/1‑150 (MQ=255)
                                                                                                                                             ttAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACAGCCCGTTGCTGGCGAACGGCTCGCGGGTGAAATCAATCTTCCATTCGCCTTTTAAt   <  2:557321/150‑1 (MQ=255)
                                                                                                                                              tagatagaCGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACAGCCCGTTGCTGGCGAACGGCTCGCGGGTGAAATCAATCTTCCATTCGCCTTTTAATg  >  2:4243792/1‑150 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
TGCAAATTACCGTCGGGTTGGTTACGCGCCCGATTCTGGCTCCAGAATGCACGGTACGCTTCACCTTTCACTTCAAACTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGAGAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACAGCCCGTTGCTGGCGAACGGCTCGCGGGTGAAATCAATCTTCCATTCGCCTTTTAATG  >  CP009273/410881‑411172

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
ACCTGCAAATTACCGTCGGGTTGGTTACGCGCCCGATTCTGGCTCCAGAATGCACGGTACGCTTCACCTTTCACTTCAAACTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGAGAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACAGCCCGTTGCTGGCGAACGGCTCGCGGGTGAAATCAATCTTCCATTCGCCTTTTAATGAGTT  >  CP009273/410878‑411176
                                                                                                                                                     |                                                                                                                                                     
ACCTGCAAATTACCGTCGGGTTGGTTACGCGCCCGATTCTGGCTCCAGAATGCACGGTACGCTTCACCTTTCACTTCAAACTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGAT                                                                                                                                                       >  SRR19973083.560666/1‑150 (MQ=60)
ACCTGCAAATTACCGTCGGGTTGGTTACGCGCCCGATTCTGGCTCCAGAATGCACGGTACGCTTCACCTTTCACTTCAAACTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGAT                                                                                                                                                       >  SRR19973083.560730/1‑150 (MQ=60)
 CCTGCAAATTACCGTCGGGTTGGTTACGCGCCCGATTCTGGCTCCAGAATGCACGGTACGCTTCACCTTTCACTTCAAACTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATA                                                                                                                                                      <  SRR19973083.4958236/150‑1 (MQ=60)
 CCTGCAAATTACCGTCGGGTTGGTTACGCGCCCGATTCTGGCTCCAGAATGCACGGTACGCTTCACCTTTCACTTCAAACTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATA                                                                                                                                                      <  SRR19973083.2740596/150‑1 (MQ=60)
 CCTGCAAATTACCGTCGGGTTGGTTACGCGCCCGATTCTGGCTCCAGAATGCACGGTACGCTTCACCTTTCACTTCAAACTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATA                                                                                                                                                      <  SRR19973083.1828126/150‑1 (MQ=60)
   TGCAAATTACCGTCGGGTTGGTTACGCGCCCGATTCTGGCTCCAGAATGCACGGTACGCTTCACCTTTCACTTCAAACTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGA                                                                                                                                                    <  SRR19973083.88601/150‑1 (MQ=60)
       AATTACCGTCGGGTTGGTTACGCGCCCGATTCTGGCTCCAGAATGCACGGTACGCTTCACCTTTCACTTCAAACTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCG                                                                                                                                                <  SRR19973083.1200646/150‑1 (MQ=60)
       AATTACCGTCGGGTTGGTTACGCGCCCGATTCTGGCTCCAGAATGCACGGTACGCTTCACCTTTCACTTCAAACTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCG                                                                                                                                                <  SRR19973083.360031/150‑1 (MQ=60)
       AATTACCGTCGGGTTGGTTACGCGCCCGATTCTGGCTCCAGAATGCACGGTACGCTTCACCTTTCACTTCAAACTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCG                                                                                                                                                <  SRR19973083.812296/150‑1 (MQ=60)
       AATGACCGTCGGGTTGGTTACGCGCCCGATTCTGGCTCCAGAATGCACGGTACGCTTCACCTTTCACTTCAAACTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCG                                                                                                                                                <  SRR19973083.359815/150‑1 (MQ=60)
          TACCGTCGGGTTGGTTACGCGCCCGATTCTGGCTCCAGAATGCACGGTACGCTTCACCTTTCACTTCAAACTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAG                                                                                                                                             >  SRR19973083.599649/1‑150 (MQ=60)
          TACCGTCGGGTTGGTTACGCGCCCGATTCTGGCTCCAGAATGCACGGTACGCTTCACCTTTCACTTCAAACTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAG                                                                                                                                             <  SRR19973083.1099716/150‑1 (MQ=60)
          TACCGTCGGGTTGGTTACGCGCCCGATTCTGGCTCCAGAATGCACGGTACGCTTCACCTTTCACTTCAAACTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAG                                                                                                                                             <  SRR19973083.1081343/150‑1 (MQ=60)
              GTCGGGTTGGTTACGCGCCCGATTCTGGCTCCAGAATGCACGGTACGCTTCACCTTTCACTTCAAACTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTC                                                                                                                                         <  SRR19973083.698963/150‑1 (MQ=60)
              GTCGGGTTGGTTACGCGCCCGATTCTGGCTCCAGAATGCACGGTACGCTTCACCTTTCACTTCAAACTCCACCTCCGCCAGACACTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTC                                                                                                                                         <  SRR19973083.4921682/150‑1 (MQ=60)
                 GGGTTGGTTACGCGCCCGATTCTGGCTCCAGAATGCACGGTACGCTTCACCTTTCACTTCAAACTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTG                                                                                                                                      >  SRR19973083.684794/1‑150 (MQ=60)
                 GGGTTGGTTACGCGCCCGATTCTGGCTCCAGAATGCACGGTACGCTTCACCTTTCACTTCAAACTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTG                                                                                                                                      >  SRR19973083.684521/1‑150 (MQ=60)
                    TTGGTTACGCGCCCGATTCTGGCTCCAGAATGCACGGTACGCTTCACCTTTCACTTCAAACTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATA                                                                                                                                   >  SRR19973083.964195/1‑150 (MQ=60)
                      GGTTACGCGCCCGATTCTGGCTCCAGAATGCACGGTACGCTTCACCTTTCACTTCAAACTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACA                                                                                                                                 <  SRR19973083.1001446/150‑1 (MQ=60)
                      GGTTACGCGCCCGATTCTGGCTCCAGAATGCACGGTACGCTTCACCTTTCACTTCAAACTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACA                                                                                                                                 <  SRR19973083.1001466/150‑1 (MQ=60)
                          ACGCGCCCGATTCTGGCTCCAGAATGCACGGTACGCTTCACCTTTCACTTCAAACTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCAGGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGC                                                                                                                             >  SRR19973083.2225738/1‑150 (MQ=60)
                             CGCCCGATTCTGGCTCCAGAATGCACGGTACGCTTCACCTTTCACTTCAAACTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAG                                                                                                                          >  SRR19973083.1283503/1‑150 (MQ=60)
                             CGCCCGATTCTGGCTCCAGAATGCACGGTACGCTTCACCTTTCACTTCAAACTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAG                                                                                                                          >  SRR19973083.3147908/1‑150 (MQ=60)
                                  GATTCTGGCTCCAGAATGCACGGTACGCTTCACCTTTCACTTCAAACTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAA                                                                                                                     >  SRR19973083.1494290/1‑150 (MQ=60)
                                  GATTCTGGCTCCAGAATGCACGGTACGCTTCACCTTTCACTTCAAACTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAA                                                                                                                     >  SRR19973083.2611531/1‑150 (MQ=60)
                                   ATTCTGGCTCCAGAATGCACGGTACGCTTCACCTTTCACTTCAAACTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAAT                                                                                                                    <  SRR19973083.3924882/150‑1 (MQ=60)
                                   ATTCTGGCTCCAGAATGCACGGTACGCTTCACCTTTCACTTCAAACTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAAT                                                                                                                    <  SRR19973083.2716178/150‑1 (MQ=60)
                                   ATTCTGGCTCCAGAATGCACGGTACGCTTCACCTTTCACTTCAAACTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGAGAGACGCGGAGTTTCGTGATACAGCGCCAGACAAAT                                                                                                                    >  SRR19973083.816994/1‑150 (MQ=60)
                                         GCTCCAGAATGCACGGTACGCTTCACCTTTCACTTCAAACTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTC                                                                                                              <  SRR19973083.1056372/150‑1 (MQ=60)
                                          CTCCAGAATGCACGGTACGCTTCACCTTTCACTTCAAACTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCC                                                                                                             <  SRR19973083.966720/150‑1 (MQ=60)
                                          CTCCAGAATGCACGGTACGCTTCACCTTTCACTTCAAACTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCC                                                                                                             <  SRR19973083.967049/150‑1 (MQ=60)
                                           TCCAGAATGCACGGTACGCTTCACCTTTCACTTCAAACTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGGTACAGCGCCAGACAAATGGCGTCCA                                                                                                            <  SRR19973083.1516020/150‑1 (MQ=60)
                                           TCCAGAATGCACGGTACGCTTCACCTTTCACTTCAAACTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCA                                                                                                            <  SRR19973083.1515859/150‑1 (MQ=60)
                                            CCAGAATGCACGGTACGCTTCACCTTTCACTTCAAACTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAG                                                                                                           >  SRR19973083.2466004/1‑150 (MQ=60)
                                             CAGAATGCACGGTACGCTTCACCTTTCACTTCAAACTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGC                                                                                                          >  SRR19973083.3254112/1‑150 (MQ=60)
                                              AGAATGCACGGTACGCTTCACCTTTCACTTCAAACTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCA                                                                                                         >  SRR19973083.850356/1‑150 (MQ=60)
                                                AATGCACGGTACGCTTCACCTTTCACTTCAAACTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGG                                                                                                       <  SRR19973083.5203687/150‑1 (MQ=60)
                                                 ATGCACGGTACGCTTCACCTTTCACTTCAAACTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGG                                                                                                      >  SRR19973083.4982484/1‑150 (MQ=60)
                                                 ATGCACGGTACGCTTCACCTTTCACTTCAAACTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGG                                                                                                      <  SRR19973083.3911350/150‑1 (MQ=60)
                                                 ATGCACGGTACGCTTCACCTTTCACTTCAAACTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGG                                                                                                      <  SRR19973083.3059881/150‑1 (MQ=60)
                                                  TGCACGGTACGCTTCACCTTTCACTTCAAACTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATAGAGCGCCAGACAAATGGCGTCCAGCAGGGT                                                                                                     >  SRR19973083.3926367/1‑150 (MQ=60)
                                                     ACGGTACGCTTCACCTTTCACTTCAAACTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGAGAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGT                                                                                                  <  SRR19973083.3286774/150‑1 (MQ=60)
                                                     ACGGTACGCTTCACCTTTCAATTCAAACTCCACCTCCGCCAGTCATTCGGCGGTATCGAGGGTCATGAGATCATTTTGCGATTGTTAAACGTTATAGAGACGCGTAGTTTGGTTATACAGCGCCAGACAAATGGCGTCCTGCAGGGTGGT                                                                                                  <  SRR19973083.3286793/150‑1 (MQ=60)
                                                      CGGTACGCTTCACCTTTCACTTCAAACTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGAGAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTT                                                                                                 <  SRR19973083.2122961/150‑1 (MQ=60)
                                                        GTACGCTTCACCTTTCACTTCAAACTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTT                                                                                               >  SRR19973083.2808737/1‑150 (MQ=60)
                                                          ACGCTTCACCTTTCACTTCAAACTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCAATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCC                                                                                             <  SRR19973083.733013/150‑1 (MQ=60)
                                                            GCTTCACCTTTCACTTCAAACTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAATTGGCGTCCAGCTGGGTGGTTTTCCCC                                                                                           <  SRR19973083.5154212/150‑1 (MQ=60)
                                                            GCTTCACCTTTCACTTCAAACTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCC                                                                                           <  SRR19973083.797327/150‑1 (MQ=60)
                                                            GCTTCACCTTTCACTTCAAACTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCC                                                                                           >  SRR19973083.3060813/1‑150 (MQ=60)
                                                            GCTTCACCTTTCACTTCAAACTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCC                                                                                           <  SRR19973083.5154228/150‑1 (MQ=60)
                                                              TTCACCTTTCACTTCAAACTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGC                                                                                         >  SRR19973083.693441/1‑150 (MQ=60)
                                                               TCACCTTTCACTTCAAACTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCA                                                                                        >  SRR19973083.2198379/1‑150 (MQ=60)
                                                                 ACCTTTCACTTCAAACTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACC                                                                                      <  SRR19973083.3773153/150‑1 (MQ=60)
                                                                  CCTTTCACTTCAAACTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCT                                                                                     >  SRR19973083.3270579/1‑150 (MQ=60)
                                                                    TTTCACTTCAAACTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACGGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGT                                                                                   >  SRR19973083.1811030/1‑150 (MQ=60)
                                                                    TTTCACTTCAAACTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGT                                                                                   >  SRR19973083.1811099/1‑150 (MQ=60)
                                                                     TTCACTTCAAACTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTT                                                                                  <  SRR19973083.1373201/150‑1 (MQ=60)
                                                                      TCACTTCAAACTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTG                                                                                 >  SRR19973083.377750/1‑150 (MQ=60)
                                                                         CTTCAAACTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGC                                                                              >  SRR19973083.5109387/1‑150 (MQ=60)
                                                                         CTTCAAACTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGC                                                                              >  SRR19973083.5109533/1‑150 (MQ=60)
                                                                             AAACTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGT                                                                          <  SRR19973083.603715/150‑1 (MQ=60)
                                                                              AACTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTA                                                                         >  SRR19973083.1154039/1‑150 (MQ=60)
                                                                               ACTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAA                                                                        <  SRR19973083.4561587/150‑1 (MQ=60)
                                                                                CTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGTGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAAT                                                                       <  SRR19973083.5210685/150‑1 (MQ=60)
                                                                                  CCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAG                                                                     <  SRR19973083.1367696/150‑1 (MQ=60)
                                                                                   CACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGC                                                                    <  SRR19973083.4949714/150‑1 (MQ=60)
                                                                                    ACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCA                                                                   <  SRR19973083.3153481/150‑1 (MQ=60)
                                                                                     CCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAA                                                                  <  SRR19973083.713515/150‑1 (MQ=60)
                                                                                     CCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAA                                                                  <  SRR19973083.713636/150‑1 (MQ=60)
                                                                                     CCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAA                                                                  <  SRR19973083.713598/150‑1 (MQ=60)
                                                                                     CCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAA                                                                  <  SRR19973083.552293/150‑1 (MQ=60)
                                                                                      CTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGAGAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAA                                                                 >  SRR19973083.4313521/1‑150 (MQ=60)
                                                                                       TCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAAC                                                                <  SRR19973083.4630832/150‑1 (MQ=60)
                                                                                       TCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAAC                                                                <  SRR19973083.2436938/150‑1 (MQ=60)
                                                                                       TCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAAC                                                                >  SRR19973083.2147170/1‑150 (MQ=60)
                                                                                       TCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAAC                                                                >  SRR19973083.4743048/1‑150 (MQ=60)
                                                                                       TCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAAC                                                                <  SRR19973083.4630669/150‑1 (MQ=60)
                                                                                          GCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACAGC                                                             >  SRR19973083.2516357/1‑150 (MQ=60)
                                                                                          GCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACAGC                                                             >  SRR19973083.1281869/1‑150 (MQ=60)
                                                                                          GCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACAGC                                                             >  SRR19973083.2516362/1‑150 (MQ=60)
                                                                                           CCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACAGCC                                                            <  SRR19973083.3627002/150‑1 (MQ=60)
                                                                                           CCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGAGAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACAGCC                                                            <  SRR19973083.5052205/150‑1 (MQ=60)
                                                                                                 ATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACAGCCCGTTGC                                                      <  SRR19973083.5040480/150‑1 (MQ=60)
                                                                                                  TTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACAGCCCGTTGCT                                                     <  SRR19973083.4440423/150‑1 (MQ=60)
                                                                                                  TTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACAGCCCGTTGCT                                                     <  SRR19973083.184210/150‑1 (MQ=60)
                                                                                                   TCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACAGCCCGTTGCTG                                                    <  SRR19973083.5058392/150‑1 (MQ=60)
                                                                                                    CGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACAGCCCGTTGCTGG                                                   >  SRR19973083.3756072/1‑150 (MQ=60)
                                                                                                    CGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACAGCCCGTTGCTGG                                                   >  SRR19973083.3755972/1‑150 (MQ=60)
                                                                                                    CGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACAGCCCGTTGCTGG                                                   <  SRR19973083.3664557/150‑1 (MQ=60)
                                                                                                     GGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACAGCCCGTTGCTGGC                                                  >  SRR19973083.2609707/1‑150 (MQ=60)
                                                                                                     GGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACAGCCCGTTGCTGGC                                                  <  SRR19973083.2701014/150‑1 (MQ=60)
                                                                                                        GGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACAGCCCGTTGCTGGCGAA                                               >  SRR19973083.3775411/1‑150 (MQ=60)
                                                                                                              GCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACAGCCCGTTGCTGGCGAACGGCTC                                         <  SRR19973083.1606253/150‑1 (MQ=60)
                                                                                                              GCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACAGCCCGTTGCTGGCGAACGGCTC                                         >  SRR19973083.78831/1‑150 (MQ=60)
                                                                                                               CGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACAGCCCGTTGCTGGCGAACGGCTCG                                        >  SRR19973083.4229055/1‑150 (MQ=60)
                                                                                                               CGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACAGCCCGTTGCTGGCGAACGGCTCG                                        >  SRR19973083.5137305/1‑150 (MQ=60)
                                                                                                                GGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACAGCCCGTTGCTGGCGAACGGCTCGC                                       >  SRR19973083.4751671/1‑150 (MQ=60)
                                                                                                                  GTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACAGCCCGTTGCTGGCGAACGGCTCGCGG                                     >  SRR19973083.2181095/1‑150 (MQ=60)
                                                                                                                   TCATGAGATCATTTTGCGATTGTGAAACGTTAGAGAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACAGCCCGTTGCTGGCGAACGGCTCGCGGG                                    <  SRR19973083.4244591/150‑1 (MQ=60)
                                                                                                                    CATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACAGCCCGTTGCTGGCGAACGGCTCGCGGGT                                   <  SRR19973083.3256090/150‑1 (MQ=60)
                                                                                                                        AGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACAGCCCGTTGCTGGCGAACGGCTCGCGGGTGAAA                               <  SRR19973083.1910639/150‑1 (MQ=60)
                                                                                                                          ATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACAGCCCGTTGCTGGCGAACGGCTCGCGGGTGAAATC                             <  SRR19973083.4323901/150‑1 (MQ=60)
                                                                                                                            CATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCTCGCACCTGTTGGGCCGGTAATAGCAAACAGCCCGTTGCTGGCGAACGGCTCGCGGGTGAAATCAA                           <  SRR19973083.1130382/150‑1 (MQ=60)
                                                                                                                            CATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACAGCCCGTTGCTGGCGAACGGCTCGCGGGTGAAATCAA                           >  SRR19973083.1468290/1‑150 (MQ=60)
                                                                                                                              TTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACAGCCCGTTGCTGGCGAACGGCTCGCGGGTGAAATCAATC                         <  SRR19973083.4763502/150‑1 (MQ=60)
                                                                                                                                    GATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACAGCCCGTTGCTGGCGAACGGCTCGCGGGTGAAATCAATCTTCCAT                   <  SRR19973083.1397068/150‑1 (MQ=60)
                                                                                                                                          GAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACAGCCCGTTGCTGGCGAACGGCTCGCGGGTGAAATCAATCTTCCATTCGCCT             >  SRR19973083.1602630/1‑150 (MQ=60)
                                                                                                                                          GAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACAGCCCGTTGCTGGCGAACGGCTCGCGGGTGAAATCAATCTTCCATTCGCCT             >  SRR19973083.1602645/1‑150 (MQ=60)
                                                                                                                                           AAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACAGCCCGTTGCTGGCGAACGGCTCGCGGGTGAAATCAATCTTCCATTCGCCTT            >  SRR19973083.1386598/1‑150 (MQ=60)
                                                                                                                                              CGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACAGCCCGTTGCTGGCGAACGGCTCGCGGGTGAAATCAATCTTCCATTCGCCTTTTA         <  SRR19973083.2447458/150‑1 (MQ=60)
                                                                                                                                              CGTTAGAGAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACAGCCCGTTGCTGGCGAACGGCTCGCGGGTGAAATCAATCTTCCATTCGCCTTTTA         <  SRR19973083.4744323/150‑1 (MQ=60)
                                                                                                                                              CGTTAGAGAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACAGCCCGTTGCTGGCGAACGGCTCGCGGGTGAAATCAATCTTCCATTCGCCTTTTA         <  SRR19973083.4744327/150‑1 (MQ=60)
                                                                                                                                               GTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACAGCCCGTTGCTGGCGAACGGCTCGCGGGTGAAATCAATCTTCCATTCGCCTTTTAA        >  SRR19973083.3956499/1‑150 (MQ=60)
                                                                                                                                               GTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACAGCCCGTTGCTGGCGAACGGCTCGCGGGTGAAATCAATCTTCCATTCGCCTTTTAA        >  SRR19973083.1726601/1‑150 (MQ=60)
                                                                                                                                                 TAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACAGCCCGTTGCTGGCGAACGGCTCGCGGGTGAAATCAATCTTCCATTCGCCTTTTAATG      >  SRR19973083.280454/1‑150 (MQ=60)
                                                                                                                                                   GATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACAGCCCGTTGCTGGCGAACGGCTCGCGGGTGAAATCAATCTTCCATTCGCCTTTTAATGAG    >  SRR19973083.4263264/1‑150 (MQ=60)
                                                                                                                                                     TAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACAGCCCGTTGCTGGCGAACGGCTCGCGGGTGAAATCAATCTTCCATTCGCCTTTTAATGAGTT  >  SRR19973083.1596599/1‑150 (MQ=60)
                                                                                                                                                     |                                                                                                                                                     
ACCTGCAAATTACCGTCGGGTTGGTTACGCGCCCGATTCTGGCTCCAGAATGCACGGTACGCTTCACCTTTCACTTCAAACTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGAGAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACAGCCCGTTGCTGGCGAACGGCTCGCGGGTGAAATCAATCTTCCATTCGCCTTTTAATGAGTT  >  CP009273/410878‑411176

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑