Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F78 I0 R1 626 241.3 10389380 77.7% 8072548 149.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 495,504 A→T 16.3% intergenic (‑75/‑77) aes ← / → gsk acetyl esterase/inosine/guanosine kinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273495,5040AT16.3% 691.1 / 119.2 245intergenic (‑75/‑77)aes/gskacetyl esterase/inosine/guanosine kinase
Reads supporting (aligned to +/- strand):  ref base A (113/92);  new base T (24/16);  total (137/108)
Fisher's exact test for biased strand distribution p-value = 6.05e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.63e-01

Only 100 of 249 total aligned reads displayed.

CTGAAGAGTATTCACAACGGTCTTCATTTCAGCAGAAATAAGGTCCAGAACAGGTAGTTTGTTTTCCGGCTTCATACAAAAACTCCTTTCAAATTACGTCATTGTAAGGAACCACTGCCATGAAAATGCGATCCCGCCTGCTGATATTGAAACTGGCTGCGTCTCGCGCGCTCCCGTCAGATTGTGTTAACATTCGCCGCTCAGTTAACCACCCGTAAAAACAACCATGAAATTTCCCGGTAAACGTAAATCCAAACATTACTTCCCCGTAAACGCACGCGATCCGCTGCTTCAGCAAT  >  CP009273/495355‑495653
                                                                                                                                                     |                                                                                                                                                     
   aaGAGTATTCACAACGGTCTTCATTTCAGCAGAAATAAGGTCCAGAACAGGTAGTTTGTTTTCCGGCTTCATACAAAAACTCCTTTCAAATTACGTCATTGTAAGGAACCACTGCCATGAAAATGCGATCCCGCCTGCTGATATTGAAAc                                                                                                                                                    >  2:529827/1‑150 (MQ=255)
    agatTATTCACAACGGTCTTCATTTCAGCAGAAATAAGGTCCAGAACAGGTAGTTTGTTTTCCTGATTCATACAAAAACTCCTTTCAAATTATGTCATTGTAAGGAACCACTGCCATGAAAATGCGATCCCGCCTGCTGATATTGAAACt                                                                                                                                                   <  2:4164999/150‑1 (MQ=255)
        tagtCACAACGGTCTTCATTTCAGCAGAAATAAGGTCCAGAACAGGTAGTTTGTTTTCCGGCTTCATACAAAAACTCCTTTCAAATTACGTCATTGTAAGGAACCACTGCCATGAAAATGCGATCCCGCCTGCTGATATTGAAACTGGCt                                                                                                                                               <  2:3702638/147‑1 (MQ=255)
         aTTCACAACGGTCTTCATTTCAGCAGAAATAAGGTCCAGAACAGGTAGTTTGTTTTCCGGCTTCATACAAAAACTCCTTTCAAATTACGTCATTGTAAGGAACCACTGCCATGAAAATGCGATCCCGCCTGCTGATATTGAAACTGGCTg                                                                                                                                              >  1:752610/1‑150 (MQ=255)
            cacaACGGTCTTCATTTCAGCAGAAATAAGGTCCAGAACAGGTAGTTTGTTTTCCGGCTTCATACAAAAACTCCTTTCAAATTACGTCATTGTAAGGAACCACTGCCATGAAAATGCGATCCCGCCTGCTGATATTGAAACTGGCTGCGt                                                                                                                                           >  2:275216/1‑150 (MQ=255)
              caACGGTCTTCATTTCAGCAGAAATAAGGTCCAGAACAGGTAGTTTGTTTTCCGGCTTCATACAAAAACTCCTTTCAAATTACGTCATTGTAAGGAACCACTGCCATGAAAATGCGATCCCGCCTGCTGATATTGAAACTGGCTGCGtct                                                                                                                                         >  1:4596985/1‑150 (MQ=255)
                 cGGTCTTCATTTCAGCAGAAATAAGGTCCAGAACAGGTAGTTTGTTTTCCGGCTTCATACAAAAACTCCTTTCAAATTACGTCATTGTAAGGAACCACTGCCATGAAAATGCGATCCCGCCTGCTGATATTGTAACTGGCTGCGTCTcgc                                                                                                                                      >  1:1016361/1‑150 (MQ=255)
                   gTCTTCATTTCAGCAGAAATAAGGTCCAGAACAGGTAGTTTGTTTTCCGGCTTCATACAAAAACTCCTTTCAAATTACGTCATTGTAAGGAACCACTGCCATGAAAATGCGATCCCGCCTGCTGATATTGAAACTGGCTGCGTCTcgcgc                                                                                                                                    >  1:4971451/1‑150 (MQ=255)
                      ttCATTTCAGCAGAAATAAGGTCCAGAACAGGTAGTTTGTTTTCCGGCTTCATACAAAAACTCCTTTCAAATTACGTCATTGTAAGGAACCACTGCCATGAAAATGCGATCCCGCCTGCTGATATTGAAACTGGCTGCGTCTCGCGCGCt                                                                                                                                 >  1:4766800/1‑150 (MQ=255)
                      ttCATTTCAGCAGAAATAAGGTCCAGAACAGGTAGTTTGTTTTCCGGCTTCATACAAAAACTCCTTTCAAATTACGTCATTGTAAGGAACCACTGCCATGAAAATGCGATCCCGCCTGCTGATATTGAAACTGGCTGCGTCTCGCGCGCt                                                                                                                                 <  1:4081033/150‑1 (MQ=255)
                         aTTTCAGCAGAAATAAGGTCCAGAACAGGTAGTTTGTTTTCCGGCTTCATACAAAAACTCCTTTCAAATTACGTCATTGTAAGGAACCACTGCCATGAAAATGCGATCCCGCCTGCTGATATTGAAACTGGCTGCGTCTCGCGCGCTccc                                                                                                                              <  1:4485568/150‑1 (MQ=255)
                         aTTTCAGCAGAAATAAGGTCCAGAACAGGTAGTTTGTTTTCCGGCTTCATACAAAAACTCCTTTCAAATTACGTCATTGTAAGGAACCACTGCCATGAAAATGCGATCCCGCCTGCTGATATTGAAACTGGCTGCGTCTCGCGCGCTccc                                                                                                                              >  2:3655039/1‑150 (MQ=255)
                           ttCAGCAGAAATAAGGTCCAGAACAGGTAGTTTGTTTTCCGGCTTCATACAAAAACTCCTTTCAAATTACGTCATTGTAAGGAACCACTGCCATGAAAATGCGATCCCGCCTGCTGATATTGAAACTGGCTGCGTCTCGCGCGCTCCCGt                                                                                                                            >  1:1543330/1‑150 (MQ=255)
                            tCAGCAGAAATAAGGTCCAGAACAGGTAGTTTGTTTTCCGGCTTCATACAAAAACTCCTTTCAAATTACGTCATTGTAAGGAACCACTGCCATGAAAATGCGATCCCGCCTGCTGATATTGAAACTGGCTGCGTCTCGCGCGCTCCCGTc                                                                                                                           <  1:4851477/150‑1 (MQ=255)
                            tCAGCAGAAATAAGGTCCAGAACAGGTAGTTTGTTTTCCGGCTTCATACAAAAACTCCTTTCAAATTACGTCATTGTAAGGAACCACTGCCATGAAAATGCGATCCCGCCTGCTGATATTGAAACTGGCTGCGTCTCGCGCGCTCCCGTc                                                                                                                           <  2:4884354/150‑1 (MQ=255)
                             cagcagAAATAAGGTCCAGAACAGGTAGTTTGTTTTCCGGCTTCATACAAAAACTCCTTTCAAATTACGTCATTGTAAGGAACCACTGCCATGAAAATGCGATCCCGCCTGCTGATATTGAAACTGGCTGCGTCTCGCGCGCTCCCGTCa                                                                                                                          >  2:3602438/1‑150 (MQ=255)
                              agcagAAATAAGGTCCAGAACAGGTAGTTTGTTTTCCGGCTTCATACAAAAACTCCTTTCAAATTACGTCATTGTAAGGAACCACTGCCATGAAAATGCGATCCCGCCTGCTGATATTGAAACTGGCTGCGTCTCGCGCGCTCCCGTCAg                                                                                                                         >  1:1053709/1‑150 (MQ=255)
                              agcagAAATAAGGTCCAGAACAGGTAGTTTGTTTTCCGGCTTCATACAAAAACTCCTTTCAAAGTACGTCATTGTAAGGAACCACTGCCATGAAAATGCGATCCCGCCTGCTGATATTGAAACTGGCTGCGTCTCGCGCGCTCCCGTCAg                                                                                                                         <  1:3690128/150‑1 (MQ=255)
                                cagAAATAAGGTCCAGAACAGGTAGTTTGTTTTCCGGCTTCATACAAAAACTCCTTTCAAATTACGTCATTGTAAGGAACCACTGCCATGAAAATGCGATCCCGCCTGCTGATATTGTAACTGGCTGCGTCTCGCGCGCTCCCGTCAGAt                                                                                                                       >  2:3328766/1‑150 (MQ=255)
                                cagAAATAAGGTCCAGAACAGGTAGTTTGTTTTCCGGCTTCATACAAAAACTCCTTTCAAATTACGTCATTGTAAGGAACCACTGCCATGAAAATGCGATCCCGCCTGCTGATATTGAAACTGGCTGCGTCTCGCGCGCTCCCGTCAGAt                                                                                                                       <  2:170008/150‑1 (MQ=255)
                                 agAAATAAGGTCCAGAACAGGGAGTTTGTTTTCCGGCTTCATACAAAAACTCCTTTCAAATTACGTCATTGTAAGGAACCACTGCCATGAAAATGCGATCCCGCCTGCTGATATTGTAACTGGCTGCGTCTCGCGCGCTCCCGTCAGAtt                                                                                                                      <  2:4940724/150‑1 (MQ=255)
                                  gAAATAAGGTCCAGAACAGGTAGTTTGTTTTCCGGCTTCATACAAAAACTCCTTTCAAATTACGTCATTGTAAGGAACCACTGCCATGAAAATGCGATCCCGCCTGCTGATATTGAAACTGGCTGCGTCTCGCGCGCTCCCGTCAGATtg                                                                                                                     >  1:314746/1‑150 (MQ=255)
                                   aaaTAAGGTCCAGAACAGGTAGTTTGTTTTCCGGCTTCATACAAAAACTCCTTTCAAATTACGTCATTGTAAGGAACCACTGCCATGAAAATGCGATCCCGCCTGCTGATATTGTAACTGGCTGCGTCTCGCGCGCTCCCGTCAGATtgt                                                                                                                    <  2:4365852/150‑1 (MQ=255)
                                   aaaTAAGGTCCAGAACAGGTAGTTTGTTTTCCGGCTTCATACAAAAACTCCTTTCAAATTACGTCATTGTAAGGAACCACTGCCATGAAAATGCGATCCCGCCTGCTGATATTGTAACTGGCTGCGTCTCGCGCGCTCCCGTCAGATtgt                                                                                                                    <  2:4235434/150‑1 (MQ=255)
                                       aaGGTCCAGAACAGGTAGTTTGTTTTCCGGCTTCATACAAAAACTCCTTTCAAATTACGTCATTGTAAGGAACCACTGCCATGAAAATGCGATCCCGCCTGCTGATATTGAAACTGGCTGCGTCTCGCGCGCTCCCGTCAGATTGTGTTa                                                                                                                >  1:3120957/1‑150 (MQ=255)
                                       aaGGTCCAGAACAGGTAGTTTGTTTTCCGGCTTCATACAAAAACTCCTTTCAAATTACGTCATTGTAAGGAACCACTGCCATGAAAATGCGATCCCGCCTGCTGATATTGAAACTGGCTGCGTCTCGCGCGCTCCCGTCAGATTGTGTTa                                                                                                                <  1:3343124/150‑1 (MQ=255)
                                        aGGTCCAGAACAGGTAGTTTGTTTTCCGGCTTCATACAAAAACTCCTTTCAAATTACGTCATTGTAAGGAACCACTGCCATGAAAATGCGATCCCGCCTGCTGATATTGAAACTGGCTGCGTCTCGCGCGCTCCCGTCAGATTGTGTTaa                                                                                                               >  2:4651060/1‑150 (MQ=255)
                                          gTCCAGAACAGGTAGTTTGTTTTCCGGCTTCATACAAAAACTCCTTTCAAATTACGTCATTGTAAGGAACCACTGCCATGAAAATGCGATCCCGCCTGCTGATATTGAAACTGGCTGCGTCTCGCGCGCTCCCGTCAGATTGTGTTAACa                                                                                                             <  2:1743867/150‑1 (MQ=255)
                                            ccAGAACAGGTAGTTTGTTTTCCGGCTTCATACAAAAACTCCTTTCAAATTACGTCATTGTAAGGAACCACTGCCATGAAAATGCGATCCCGCCTGCTGATATTGTAACTGGCTGCGTCTCGCGCGCTCCCGTCAGATTGTGTTAACAtt                                                                                                           >  1:1867507/1‑150 (MQ=255)
                                              aGAACAGGTAGTTTGTTTTCCGGCTTCATACAAAAACTCCTTTCAAATTACGTCATTGTAAGGAACCACTGCCATGAAAATGCGATCCCGCCTGCTGATATTGAAACTGGCTGCGTCTCGCGCGCTCCCGTCAGATTGTGTTAACATTcg                                                                                                         >  1:3447299/1‑150 (MQ=255)
                                              aGAACAGGTAGTTTGTTTTCCGGCTTCATACAAAAACTCCTTTCAAATTACGTCATTGTAAGGAACCACTGCCATGAAAATGCGATCCCGCCTGCTGATATTGAAACTGGCTGCGTCTCGCGCGCTCCCGTCAGATTGTGTTAACATTcg                                                                                                         >  1:224499/1‑150 (MQ=255)
                                                   aGGTAGTTTGTTTTCCGGCTTCATACAAAAACTCCTTTCAAATTACGTCATTGTAAGGAACCACTGCCATGAAAATGCGATCCCGCCTGCTGATATTGAAACTGGCTGCGTCTCGCGCGCTCCCGTCAGATTGTGTTAACATTCGCCGCt                                                                                                    <  2:535444/150‑1 (MQ=255)
                                                   aGGTAGTTTGTTTTCCGGCTTCATACAAAAACTCCTTTCAAATTACGTCATTGTAAGGAACCACTGCCATGAAAATGCGATCCCGCCTGCTGATATTGAAACTGGCTGCGTCTCGCGCGCTCCCGTCAGATTGTGTTAACATTCGCCGCt                                                                                                    >  1:2967397/1‑150 (MQ=255)
                                                    ggTAGTTTGTTTTCCGGCTTCATACAAAAACTCCTTTCAAATTACGTCATTGTAAGGAACCACTGCCATGAAAATGCGATCCCGCCTGCTGATATTGAAACTGGCTGCGTCTCGCGCGCTCCCGTCAGATTGTGTTAACATTCGCCGCTc                                                                                                   <  1:3295832/150‑1 (MQ=255)
                                                    ggTAGTTTGTTTTCCGGCTTCATACAAAAACTCCTTTCAAATTACGTCATTGTAAGGAACCACTGCCATGAAAATGCGATCCCGCCTGCTGATATTGAAACTGGCTGCGTCTCGCGCGCTCCCGTCAGATTGTGTTAACATTCGCCGCTc                                                                                                   >  1:2565685/1‑150 (MQ=255)
                                                    ggTAGTTTGTTTTCCGGCTTCATACAAAAACTCCTTTCAAATTACGTCATTGTAAGGAACCACTGCCATGAAAATGCGATCCCGCCTGCTGATATTGAAACTGGCTGCGTCTCGCGCGCTCCCGTCAGATTGTGTTAACATTCGCCGCTc                                                                                                   <  1:1133175/150‑1 (MQ=255)
                                                       aGTTTGTTTTCCGGCTTCATACAAAAACTCCTTTCAAATTACGTCATTGTAAGGAACCACTGCCATGAAAATGCGATCCCGCCTGCTGATATTGAAACTGGCTGCGTCTCGCGCGCTCCCGTCAGATTGTGTTAACATTCGCCGCTCAGt                                                                                                >  1:5094026/1‑150 (MQ=255)
                                                        gtttgtttTCCGGCTTCATACAAAAACTCCTTTCAAATTACGTCATTGTAAGGAACCACTGCCATGAAAATGCGATCCCGCCTGCTGATATTGAAACTGGCTGCGTCTCGCGCGCTCCCGTCAGATTGTGTTAACATTCGCCGCTCAGtt                                                                                               <  1:1059737/150‑1 (MQ=255)
                                                        gtttgttgTCCGGCTTCATACAAAAACTCCTTTCAAATTACGTCATTGTAAGGAACCACTGCCATGAAAATGCGATCCCGCCTGCTGATATTGAAACTGGCTGCGTCTCGCGCGCTCCCGTCAGATTGTGTTAACATTCGCCGCTCAGtt                                                                                               <  2:2803854/150‑1 (MQ=255)
                                                         tttgtttTCCGGCTTCATACAAAAACTCCTTTCAAATTACGTCATTGTAAGGAACCACTGCCATGAAAATGCGATCCCGCCTGCTGATATTGAAACTGGCTGCGTCTCGCGCGCTCCCGTCAGATTGTGTTAACATTCGCCGCTCAGTTa                                                                                              >  1:4071492/1‑150 (MQ=255)
                                                             ttttCCGGCTTCATACAAAAACTCCTTTCAAATTACGTCATTGTAAGGAACCACTGCCATGAAAATGCGATCCCGCCTGCTGATATTGTAACTGGCTGCGTCTCGCGCGCTCCCGTCAGATTGTGTTAACATTCGCCGCTCAGTTAacca                                                                                          <  1:574220/150‑1 (MQ=255)
                                                                tCCGGCTTCATACAAAAACTCCTTTCAAATTACGTCATTGTAAGGAACCACTGCCATGAAAATGCGATCCCGCCTGCTGATATTGAAACTGGCTGCGTCTCGCGCGCTCCCGTCAGATTGTGTTAACATTCGCCGCTCAGTTAACCAccc                                                                                       <  2:4502825/150‑1 (MQ=255)
                                                                tCCGGCTTCATACAAAAACTCCTTTCAAATTACGTCATTGTAAGGAACCACTGCCATGAAAATGCGATCCCGCCTGCTGATATTGAAACTGGCTGCGTCTCGCGCGCTCCCGTCAGATTGTGTTAACATTCGCCGCTCAGTTAACCAccc                                                                                       >  1:1683608/1‑150 (MQ=255)
                                                                      ttCATACAAAAACTCCTTTCAAATTACGTCATTGTAAGGAACCACTGCCATGAAAATGCGATCCCGCCTGCTGATATTGAAACTGGCTGCGTCTCGCGCGCTCCCGTCAGATTGTGTTAACATTCGCCGCTCAGTTAACCACCCGTaaaa                                                                                 >  2:11978/1‑150 (MQ=255)
                                                                         aTACAAAAACTCCTTTCAAATTACGTCATTGTAAGGAACCACTGCCATGAAAATGCGATCCCGCCTGCTGATATTGAAACTGGCTGCGTCTCGCGCGCTCCCGTCAGATTGTGTTAACATTCGCCGCTCAGTTAACCACCCGTAAAaaca                                                                              <  1:5172684/150‑1 (MQ=255)
                                                                          tACAAAAACTCCTTTCAAATTACGTCATTGTAAGGAACCACTGCCATGAAAATGCGATCCCGCCTGCTGATATTGAAACTGGCTGCGTCTCGCGCGCTCCCGTCAGATTGTGTTAACATTCGCCGCTCAGTTAACCACCCGTAAAaacaa                                                                             >  2:1016397/1‑150 (MQ=255)
                                                                          tACAAAAACTCCTTTCAAATTACGTCATTGTAAGGAACCACTGCCATGAAAATGCGATCCCGCCTGCTGATATTGAAACTGGCTGCGTCTCGCGCGCTCCCGTCAGATTGTGTTAACATTCGCCGCTCAGTTAACCACCCGTAAAaacaa                                                                             >  2:1399953/1‑150 (MQ=255)
                                                                             aaaaaCTCCTTTCAAATTACGTCATTGTAAGGAACCACTGCCATGAAAATGCGATCCCGCCTGCTGATATTGAAACTGGCTGCGTCTCGCGCGCTCCCGTCAGATTGTGTTAACATTCGCCGCTCAGTTAACCACCCGTAAAAACAACCa                                                                          <  2:1941060/150‑1 (MQ=255)
                                                                              aaaaCTCCTTTCAAATTACGTCATTGTAAGGAACCACTGCCATGAAAATGCGATCCCGCCTGCTGATATTGAAACTGGCTGCGTCTCGCGCGCTCCCGTCAGATTGTGTTAACATTCGCCGCTCAGTTAACCACCCGTAAAAACAACCAt                                                                         >  2:2424883/1‑150 (MQ=255)
                                                                              aaaaCTCCTTTCAAATTACGTCATTGTAAGGAACCACTGCCATGAAAATGCGATCCCGCCTGCTGATATTGAAACTGGCTGCGTCTCGCGCGCTCCCGTCAGATTGTGTTAACATTCGCCGCTCAGTTAACCACCCGTAAAAACAACCAt                                                                         >  2:1827719/1‑150 (MQ=255)
                                                                              aaaaCTCCTTTCAAATTACGTCATTGTAAGGAACCACTGCCATGAAAATGCGATCCCGCCTGCTGATATTGAAACTGGCTGCGTCTCGCGCGCTCCCGTCAGATTGTGTTAACATTCGCCGCTCAGTTAACCACCCGTAAAAACAACCAt                                                                         >  2:2633268/1‑150 (MQ=255)
                                                                              aaaaCTCCTTTCAAATTACGTCATTGTAAGGAACCACTGCCATGAAAATGCGATCCCGCCTGCTGATATTGAAACTGGCTGCGTCTCGCGCGCTCCCGTCAGATTGTGTTAACATTCGCCGCTCAGTTAACCACCCGTAAAAACAACCAt                                                                         >  2:4293348/1‑150 (MQ=255)
                                                                               aaaCTCCTTTCAAATTACGTCATTGTAAGGAACCACTGCCATGAAAATGCGATCCCGCCTGCTGATATTGAAACTGGCTGCGTCTCGCGCGCTCCCGTCAGATTGTGTTAACATTCGCCGCTCAGTTAACCACCCGTAAAAACAACCATg                                                                        >  1:2829012/1‑150 (MQ=255)
                                                                               aaaCTCCTTTCAAATTACGTCATTGTAAGGAACCACTGCCATGAAAATGCGATCCCGCCTGCTGATATTGAAACTGGCTGCGTCTCGCGCGCTCCCGTCAGATTGTGTTAACATTCGCCGCTCAGTTAACCACCCGTAAAAACAACCATg                                                                        <  1:4835442/150‑1 (MQ=255)
                                                                               aaaCTCCTTTCAAATTACGTCATTGTAAGGAACCACTGCCATGAAAATGCGATCCCGCCTGCTGATATTGAAACTGGCTGCGTCTCGCGCGCTCCCGTCAGATTGTGTTAACATTCGCCGCTCAGTTAACCACCCGTAAAAACAACCATg                                                                        >  1:466290/1‑150 (MQ=255)
                                                                                  cTCCTTTCAAATTACGTCATTGTAAGGAACCACTGCCATGAAAATGCGATCCCGCCTGCTGATATTGAAACTGGCTGCGTCTCGCGCGCTCCCGTCCGATTGTGTTCACATTCGCCGCTCAGTTAACCACCCGTAAAAACAACCAGGaaa                                                                     >  2:2726916/1‑150 (MQ=255)
                                                                                   tCCTTTCAAATTACGTCATTGTAAGGAACCACTGCCATGAAAATGCGATCCCGCCTGCTGATATTGAAACTGGCTGCGTCTCGCGCGCTCCCGTCAGATTGTGTTAACATTCGCCGCTCAGTTAACCACCCGTAAAAACAACCATGAAAt                                                                    >  2:4404888/1‑150 (MQ=255)
                                                                                    ccTTTCAAATTACGTCATTGTAAGGAACCACTGCCATGAAAATGCGATCCCGCCTGCTGATATTGAAACTGGCTGCGTCTCGCGCGCTCCCGTCAGATTGTGTTAACATTCGCCGCTCAGTTAACCACCCGTAAAAACAACCATGAAAtt                                                                   >  1:2159635/1‑150 (MQ=255)
                                                                                    ccTTTCAAATTACGTCATTGTAAGGAACCACTGCCATGAAAATGCGATCCCGCCTGCTGATATTGAAACTGGCTGCGTCTCGCGCGCTCCCGTCAGATTGTGTTAACATTCGCCGCTCAGTTAACCACCCGTAAAAACAACCATGAAAtt                                                                   >  1:3382853/1‑150 (MQ=255)
                                                                                       ttCAAATTACGTCATTGTAAGGAACCACTGCCATGAAAATGCGATCCCGCCTGCTGATATTGAAACTGGCTGCGTCTCGCGCGCTCCCGTCAGATTGTGTTAACATTCGCCGCTCAGTTAACCACCCGTAAAAACAACCATGAAATTTcc                                                                <  2:758302/150‑1 (MQ=255)
                                                                                       ttCAAATTACGTCATTGTAAGGAACCACTGCCATGAAAATGCGATCCCGCCTGCTGATATTGAAACTGGCTGCGTCTCGCGCGCTCCCGTCAGATTGTGTTAACATTCGCCGCTCAGTTAACCACCCGTAAAAACAACCATGAAATTTcc                                                                <  2:667388/150‑1 (MQ=255)
                                                                                        tCAAATTACGTCATTGTAAGGAACCACTGCCATGAAAATGCGATCCCGCCTGCTGATATTGTAACTGGCTGCGTCTCGCGCGCTCCCGTCAGATTGTGTTAACATTCGCCGCTCAGTTAACCACCCGTAAAAACAACCATGAAATTTccc                                                               >  2:2245821/1‑150 (MQ=255)
                                                                                        tCAAATTACGTCATTGTAAGGAACCACTGCCATGAAAATGCGATCCCGCCTGCTGATATTGAAACTGGCTGCGTCTCGCGCGCTCCCGTCAGATTGTGTTAACATTCGCCGCTCAGTTAACCACCCGTAAAAACAACCATGAAATTTccc                                                               <  1:1446586/150‑1 (MQ=255)
                                                                                              tACGTCATTGTAAGGAACCACTGCCATGAAAATGCGATCCCGCCTGCTGATATTGAAACTGGCTGCGTCTCGCGCGCTCCCGTCAGATTGTGTTAACATTCGCCGCTCAGTTAACCACCCGTAAAAACAACCATGAAATTTCCCGGTaaa                                                         >  1:4224447/1‑150 (MQ=255)
                                                                                              tACGTCATTGTAAGGAACCACTGCCATGAAAATGCGATCCCGCCTGCTGATATTGAAACTGGCTGCGTCTCGCGCGCTCCCGTCAGATTGTGTTAACATTCGCCGCTCAGTTAACCACCCGTAAAAACAACCATGAAATTTCCCGGTaaa                                                         >  2:2332410/1‑150 (MQ=255)
                                                                                               aCGTCATTGTAAGGAACCACTGCCATGAAAATGCGATCCCGCCTGCTGATATTGAAACTGGCTGCGTCTCGCGCGCTCCCGTCAGATTGTGTTAACATTCGCCGCTCAGTTAACCACCCGTAAAAACAACCATGAAATTTCCCGGTAAAc                                                        <  2:4714434/150‑1 (MQ=255)
                                                                                               aCGTCATTGTAAGGAACCACTGCCATGAAAATGCGATCCCGCCTGCTGATATTGAAACTGGCTGCGTCTCGCGCGCTCCCGTCAGATTGTGTTAACATTCGCCGCTCAGTTAACCACCCGTAAAAACAACCATGAAATTTCCCGGTAAAc                                                        <  1:3523431/150‑1 (MQ=255)
                                                                                               aCGTCATTGTAAGGAACCACTGCCATGAAAATGCGATCCCGCCTGCTGATATTGAAACTGGCTGCGTCTCGCGCGCTCCCGTCAGATTGTGTTAACATTCGCCGCTCAGTTAACCACCCGTAAAAACAACCATGAAATTTCCCGGTAAAc                                                        <  1:3479123/150‑1 (MQ=255)
                                                                                               aCGTCATTGTAAGGAACCACTGCCATGAAAATGCGATCCCGCCTGCTGATATTGAAACTGGCTGCGTCTCGCGCGCTCCCGTCAGATTGTGTTAACATTCGCCGCTCAGTTAACCACCCGTAAAAACAACCATGAAATTTCCCGGTAAAc                                                        <  1:924072/150‑1 (MQ=255)
                                                                                                      tGTAAGGAACCACTGCCATGAAAATGCGATCCCGCCTGCTGATATTGTAACTGGCTGCGTCTCGCGCGCTCCCGTCAGATTGTGTTAACATTCGCCGCTCAGTTAACCACCCGTAAAAACAACCATGAAATTTCCCGGTAAACGTAAATc                                                 >  2:2107401/1‑150 (MQ=255)
                                                                                                      tGTAAGGAACCACTGCCATGAAAATGCGATCCCGCCTGCTGATATTGTAACTGGCTGCGTCTCGCGCGCTCCCGTCAGATTGTGTTAACATTCGCCGCTCAGTTAACCACCCGTAAAAACAACCATGAAATTTCCCGGTAAACGTAAATc                                                 >  1:2085648/1‑150 (MQ=255)
                                                                                                       gTAAGGAACCACTGCCATGAAAATGCGATCCCGCCTGCTGATATTGAAACTGGCTGCGTCTCGCGCGCTCCCGTCAGATTGTGTTAACATTCGCCGCTCAGTTAACCACCCGTAAAAACAACCATGAAATTTCCCGGTAAACGTAAATcc                                                >  1:3935519/1‑150 (MQ=255)
                                                                                                        tAAGGAACCACTGCCATGAAAATGCGATCCCGCCTGCTGATATTGTAACTGGCTGCGTCTCGCGCGCTCCCGTCAGATTGTGTTAACATTCGCCGCTCAGTTAACCACCCGTAAAAACAACCATGAAATTTCCCGGTAAACGTAAATCCa                                               >  1:4771426/1‑150 (MQ=255)
                                                                                                           ggAACCACTGCCATGAAAATGCGATCCCGCCTGCTGATATTGAAACTGGCTGCGTCTCGCGCGCTCCCGTCAGATTGTGTTAACATTCGCCGCTCAGTTAACCACCCGTAAAAACAACCATGAAATTTCCCGGTAAACGTAAATCCAAAc                                            <  2:1429974/150‑1 (MQ=255)
                                                                                                           ggAACCACTGCCATGAAAATGCGATCCCGCCTGCTGATATTGAAACTGGCTGCGTCTCGCGCGCTCCCGTCAGATTGTGTTAACATTCGCCGCTCAGTTAACCACCCGTAAAAACAACCATGAAATTTCCCGGTAAACGTAAATCCAAAc                                            >  1:3976645/1‑150 (MQ=255)
                                                                                                            gAACCACTGCCATGAAAATGCGATCCCGCCTGCTGATATTGTAACTGGCTGCGTCTCGCGCGCTCCCGTCAGATTGTGTTAACATTCGCCGCTCAGTTAACCACCCGTAAAAACAACCATGAAATTTCCCGGTAAACGTAAATCCAAACa                                           >  1:4255602/1‑150 (MQ=255)
                                                                                                               ccACTGCCATGAAAATGCGATCCCGCCTGCTGATATTGAAACTGGCTGCGTCTCGCGCGCTCCCGTCAGATTGTGTTAACATTCGCCGCTCAGTTAACCACCCGTAAAAACAACCATGAAATTTCCCGGTAAACGTAAATCCAAACATTa                                        <  2:1091656/150‑1 (MQ=255)
                                                                                                                 aCTGCCATGAAAATGCGATCCCGCCTGCTGATATTGTAACTGGCTGCGTCTCGCGCGCTCCCGTCAGATTGTGTTAACATTCGCCGCTCAGTTAAACACCCGTAAAAACAACCATGAAATTTCACGGTAAACTTAAATCCAAACATTACt                                      >  1:2690400/1‑150 (MQ=255)
                                                                                                                  cTGCCATGAAAATGCGATCCCGCCTGCTGATATTGAAACTGGCTGCGTCTCGCGCGCTCCCGTCAGATTGTGTTAACATTCGCCGCTCAGTTAACCACCCGTAAAAACAACCATGAAATTTCCCGGTAAACGTAAATCCAAACATTACtt                                     >  1:1623515/1‑150 (MQ=255)
                                                                                                                   tGCCATGAAAATGCGATCCCGCCTGCTGATATTGTAACTGGCTGCGTCTCGCGCGCTCCCGTCAGATTGTGTTAACATTCGCCGCTCAGTTAACCACCCGTAAAAACAACCATGAAATTTCCCGGTAAACGTAAATCCAAACATTACTTc                                    >  1:2771998/1‑150 (MQ=255)
                                                                                                                       aTGAAAATGCGATCCCGCCTGCTGATATTGAAACTGGCTGCGTCTCGCGCGCTCCCGTCAGATTGTGTTAACATTCGCCGCTCAGTTAACCACCCGTAAAAACAACCATGAAATTTCCCGGTAAACGTAAATCCAAACATTACTTCCCCg                                >  2:1583021/1‑150 (MQ=255)
                                                                                                                         gAAAATGCGATCCCGCCTGCTGATATTGTAACTGGCTGCGTCTCGCGCGCTCCCGTCAGATTGTGTTAACATTCGCCGCTCAGTTAACCACCCGTAAAAACAACCATGAAATTTCCCGGTAAACGTAAATCCAAACATTACTTCCCCGTa                              >  1:3797988/1‑150 (MQ=255)
                                                                                                                         gAAAATGCGATCCCGCCTGCTGATATTGTAACTGGCTGCGTCTCGCGCGCTCCCGTCAGATTGTGTTAACATTCGCCGCTCAGTTAACCACCCGTAAAAACAACCATGAAATTTCCCGGTAAACGTAAATCCAAACATTACTTCCCCGTa                              <  2:3846262/150‑1 (MQ=255)
                                                                                                                          aaaaTGCGATCCCGCCTGCTGATATTGAAACTGGCTGCGTCTCGCGCGCTCCCGTCAGATTGTGTTAACATTCGCCGCTCAGTTAACCACCCGTAAAAACAACCATGAAATTTCCCGGTAAACGTAAATCCAAACATTACTTCCCCGTaa                             >  2:3268516/1‑150 (MQ=255)
                                                                                                                           aaaTGCGATCCCGCCTGCTGATATTGAAACTGGCTGCGTCTCGCGCGCTCCCGTCAGATTGTGTTAACATTCGCCGCTCAGTTAACCACCCGTAAAAACAACCATGAAATTTCCCGGTAAACGTAAATCCAAACATTACTTCCCCGTaaa                            <  2:605540/150‑1 (MQ=255)
                                                                                                                              tGCGATCCCGCCTGCTGATATTGAAACTGGCTGCGTCTCGCGCGCTCCCGTCAGATTGTGTTAACATTCGCCGCTCAGTTAACCACCCGTAAAAACAACCATGAAATTTCCCGGTAAACGTAAATCCAAACATTACTTCCCCGTAAacgc                         >  1:4540112/1‑150 (MQ=255)
                                                                                                                               gCGATCCCGCCTGCTGATATTGAAACTGGCTGCGTCTCGCGCGCTCCCGTCAGATTGTGTTAACATTCGCCGCTCAGTTAACCACCCGTAAAAACAACCATGAAATTTCCCGGTAAACGTAAATCCAAACATTACTTCCCCGTAAacgca                        >  1:3236848/1‑150 (MQ=255)
                                                                                                                               gCGATCCCGCCTGCTGATATTGAAACTGGCTGCGTCTCGCGCGCTCCCGTCAGATTGTGTTAACATTCGCCGCTCAGTTAACCACCCGTAAAAACAACCATGAAATTTCCCGGTAAACGTAAATCCAAACATTACTTCCCCGTAAacgca                        <  2:2560487/150‑1 (MQ=255)
                                                                                                                                cGATCCCGCCTGCTGATATTGAAACTGGCTGCGTCTCGCGCGCTCCCGTCAGATTGTGTTAACATTCGCCGCTCAGTTAACCACCCGTAAAAACAACCATGAAATTTCCCGGTAAACGTAAATCCAAACATTACTTCCCCGTAAacgcac                       <  1:1738370/150‑1 (MQ=255)
                                                                                                                                 gATCCCGCCTGCTGATATTGAAACTGGCTGCGTCTCGCGCGCTCCCGTCAGATTGTGTTAACATTCGCCGCTCAGTTAACCACCCGTAAAAACAACCATGAAATTTCCCGGTAAACGTAAATCCAAACATTACTTCCCCGTAAacgcacg                      >  1:736846/1‑150 (MQ=255)
                                                                                                                                   tCCCGCCTGCTGATATTGAAACTGGCTGCGTCTCGCGCGCTCCCGTCAGATTGTGTTAACATTCGCCGCTCAGTTAACCACCCGTAAAAACAACCATGAAATTTCCCGGTAAACGTAAATCCAAACATTACTTCCCCGTAAACGCAcgcg                    >  1:1262545/1‑150 (MQ=255)
                                                                                                                                   tCCCGCCTGCTGATATTGAAACTGGCTGCGTCTCGCGCGCTCCCGTCAGATTGTGTTAACATTCGCCGCTCAGTTAACCACCCGTAAAAACAACCATGAAATTTCCCGGTAAACGTAAATCCAAACATTACTTCCCCGTAAACGCAcgcg                    >  2:1607485/1‑150 (MQ=255)
                                                                                                                                    cccGCCTGCTGATATTGAAACTGGCTGCGTCTCGCGCGCTCCCGTCAGATTGTGTTAACATTCGCCGCTCAGTTAACCACCCGTAAAAACAACCATGAAATTTCCCGGTAAACGTAAATCCAACCATTACTTCCCCGTAAACGCACGCGa                   <  2:3233963/150‑1 (MQ=255)
                                                                                                                                     ccGCCTGCTGATATTGTAACTGGCTGCGTCTCGCGCGCTCCCGTCAGATTGTGTTAACATTCGCCGCTCAGTTAACCACCCGTAAAAACAACCATGAAATTTCCCGGTAAACGTAAATCCAAACATTACTTCCCCGTAAACGCACGCGAt                  <  1:3619802/150‑1 (MQ=255)
                                                                                                                                        ccTGCTGATATTGTAACTGGCTGCGTCTCGCGCGCTCCCGTCAGATTGTGTTAACATTCGCCGCTCAGTTAACCACCCGTAAAAACAACCATGAAATTTCCCGGTAAACGTAAATCCAAACATTACTTCCCCGTAAACGCACGCGATCCg               >  2:2195774/1‑150 (MQ=255)
                                                                                                                                          tgctgATATTGAAACTGGCTGCGTCTCGCGCGCTCCCGTCAGATTGTGTTAACATTCGCCGCTCAGTTAACCACCCGTAAAAACAACCATGAAATTTCCCGGTAAACGTAAATCCAAACATTACTTCCCCGTAAACGCACGCGATCCgct             <  2:2448106/150‑1 (MQ=255)
                                                                                                                                               atatTGAAACTGGCTGCGTCTCGCGCGCTCCCGTCAGATTGTGTTAACATTCGCCGCTCAGTTAACCACCCGTAAAAACAACCATGAAATTTCCCGGTAAACGTAAATCCAAACATTACTTCCCCGTAAACGCACGCGATCCGCTGCTTc        >  1:2321321/1‑150 (MQ=255)
                                                                                                                                                tatTGAAACTGGCTGCGTCTCGCGCGCTCCCGTCAGATTGTGTTAACATTCGCCGCTCAGTTAACCACCCGTAAAAACAACCATGAAATTTCCCGGTAAACGTAAATCCAAACATTACTTCCCCGTAAACGCACGCGATCCGCTGCTTCa       <  1:3647345/150‑1 (MQ=255)
                                                                                                                                                tatTGAAACTGGCTGCGTCTCGCGCGCTCCCGTCAGATTGTGTTAACATTCGCCGCTCAGTTAACCACCCGTAAAAACAACCATGAAATTTCCCGGTAAACGTAAATCCAAACATTACTTCCCCGTAAACGCACGCGATCCGCTGCTTCa       <  2:388907/150‑1 (MQ=255)
                                                                                                                                                     aaaCTGGCTGCGTCTCGCGCGCTCCCGTCAGATTGTGTTAACATTCGCCGCTCAGTTAACCACCCGTAAAAACAACCATGAAATTTCCCGGTAAACGTAAATCCAAACATTACTTCCCCGTAAACGCACGCGATCCGCTGCTTCAGCAAt  >  1:558693/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
CTGAAGAGTATTCACAACGGTCTTCATTTCAGCAGAAATAAGGTCCAGAACAGGTAGTTTGTTTTCCGGCTTCATACAAAAACTCCTTTCAAATTACGTCATTGTAAGGAACCACTGCCATGAAAATGCGATCCCGCCTGCTGATATTGAAACTGGCTGCGTCTCGCGCGCTCCCGTCAGATTGTGTTAACATTCGCCGCTCAGTTAACCACCCGTAAAAACAACCATGAAATTTCCCGGTAAACGTAAATCCAAACATTACTTCCCCGTAAACGCACGCGATCCGCTGCTTCAGCAAT  >  CP009273/495355‑495653

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A