Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F78 I0 R1 626 241.3 10389380 77.7% 8072548 149.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,035,236 A→T 16.6% K127N (AAA→AAT)  zinT → zinc and cadmium binding protein, periplasmic

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,035,2360AT16.6% 474.4 / 87.2 175K127N (AAA→AAT) zinTzinc and cadmium binding protein, periplasmic
Reads supporting (aligned to +/- strand):  ref base A (79/67);  new base T (17/12);  total (96/79)
Fisher's exact test for biased strand distribution p-value = 6.88e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.00e-01

Only 100 of 179 total aligned reads displayed.

TTCAGAAGAAAGCGGATGCAGATAAAACTAAAACATTTGCTGAAATTAAAGATTATTATCACAAAGGTTATGCAACAGATATCGAGATGATTGGCATTGAGGACGGCATTGTTGAATTCCATAGAAATAATGAAACAACATCGTGTAAATATGATTACGATGGATACAAAATACTCACCTATAAATCAGGCAAGAAAGGCGTTCGTTACTTATTCGAATGTAAAGATCCTGAAAGCAAAGCCCCTAAATATATACAATTTAGCGATCATATAATTGCACCACGAAAATCATCTCATT  >  CP009273/2035088‑2035384
                                                                                                                                                    |                                                                                                                                                    
 taagaagaAAGCGGATGCAGATAAAACTAAAACATTTGCTGAAATTAAAGATTATTATCACAAAGGTTATGCAACAGATATCGAGATGATTGGCATTGAGGACGGCATTGTTGAATTCCATAGAAATAATGAAACAACATCGTGTAAata                                                                                                                                                    >  2:4967950/3‑150 (MQ=255)
 tCAGAAGAAAGCGGATGCAGATAAAACTAAAACATTTGCTGAAATTAAAGATTATTATCACAAAGGTTATGCAACAGATATCGAGATGATTGGCATTGAGGACGGCATTGTTGAATTCCATAGAAATAATGAAACAACATCGTGTAAata                                                                                                                                                    <  1:1664920/150‑1 (MQ=255)
      agaaAGCGGATGCAGATAAAACTAAAACATTTGCTGAAATTAAAGATTATTATCACAAAGGTTATGCAACAGATATCGAGATGATTGGCATTGAGGACGGCATTGTTGAATTCCATAGAAATAATGAAACAACATCGTGTAAATATGAtt                                                                                                                                               >  1:1380334/1‑150 (MQ=255)
      agaaAGCGGATGCAGATAAAACTAAAACATTTGCTGAAATTAAAGATTATTATCACAAAGGTTATGCAACAGATATCGAGATGATTGGCATTGAGGACGGCATTGTTGAATTCCATAGAAATAATGAAACAACATCGTGTAAATATGAtt                                                                                                                                               >  2:863705/1‑150 (MQ=255)
       gaaAGCGGATGCAGATAAAACTAAAACATTTGCTGAAATTAAAGATTATTATCACAAAGGTTATGCAACAGATATCGAGATGATTGGCATTGAGGACGGCATTGTTGAATTCCATAGAAATAATGAAACAACATCGTGTAATTATGATTa                                                                                                                                              >  2:2084496/1‑150 (MQ=255)
       gaaAGCGGATGCAGATAAAACTAAAACATTTGCTGAAATTAAAGATTATTATCACAAAGGTTATGCAACAGATATCGAGATGATTGGCATTGAGGACGGCATTGTTGAATTCCATAGAAATAATGAAACAACATCGTGTAAATATGATTa                                                                                                                                              <  1:3200687/150‑1 (MQ=255)
         aaGCGGATGCAGATAAAACTAAAACATTTGCTGAAATTAAAGATTATTATCACAAAGGTTATGCAACAGATATCGAGATGATTGGCATTGAGGACGGCATTGTTGAATTCCATAGAAATAATGAAACAACATCGTGTAAATATGATTACg                                                                                                                                            >  2:2926588/1‑150 (MQ=255)
         aaGCGGATGCAGATAAAACTAAAACATTTGCTGAAATTAAAGATTATTATCACAAAGGTTATGCAACAGATATCGAGATGATTGGCATTGAGGACGGCATTGTTGAATTCCATAGAAATAATGAAACAACATCGTGTAAATATGATTACg                                                                                                                                            >  2:4440498/1‑150 (MQ=255)
             ggATGCAGATAAAACTAAAACATTTGCTGAAATTAAAGATTATTATCACAAAGGTTATGCAACAGATATCGAGATGATTGGCATTGAGGACGGCATTGTTGAATTCCATAGAAATAATGAAACAA‑ATCGTGTAAATATGATTACGATGGa                                                                                                                                       <  2:3340110/150‑1 (MQ=255)
              gATGCAGATAAAACTAAAACATTTGCTGAAATTAAAGATTATTATCACAAAGGTTATGCAACAGATATCGAGATGATTGGCATTGAGGACGGCATTGTTGAATTCCATAGAAATAATGAAACAACATCGTGTAAATATGATTACGATGGa                                                                                                                                       <  1:1270744/150‑1 (MQ=255)
                   tgATAAAACTAAAACATTTGCTGAAATTAAAGATTATTATCACAAAGGTTATGCAACAGATATCGAGATGATTGGCATTGAGGACGGCATTGTTGAATTCCATAGAAATAATGAAACAACATCGTGTAAATATGATTACGATGGATACaa                                                                                                                                  >  1:2817476/2‑150 (MQ=255)
                   aGATAAAACTAAAACATTTGCTGAAATTAAAGATTATTATCACAAAGGTTATGCAACAGATATCGAGATGATTGGCATTGAGGACGGCATTGTTGAATTCCATAGAAATAATGAAACAACATCGTGTAAATATGATTACGATGGATACaa                                                                                                                                  <  1:861904/150‑1 (MQ=255)
                    gATAAAACTAAAACATTTGCTGAAATTAAAGATTATTATCACAAAGGTTATGCAACAGATATCGAGATGATTGGCATTGAGGACGGCATTGTTGAATTCCATAGAAATAATGAAACAACATCGTGTAAATATGATTACGATGGATACaaa                                                                                                                                 <  2:222404/150‑1 (MQ=255)
                      taaaactaaaacATTTGCTGAAATTAAAGATTATTATCACAAAGGTTATGCAACAGATATCGAGATGATTGGCATTGAGGACGGCATTGTTGAATTCCATAGAAATAATGAAACAACATCGTGTAAATATGATTACGATGGATACAAAAt                                                                                                                               >  2:301354/1‑150 (MQ=255)
                         aactaaaacATTTGCTGAAATTAAAGATTATTATCACAAAGGTTATGCAACAGATATCGAGATGATTGGCATTGAGGACGGCATTGTTGAATTCCATAGAAATAATGAAACAACATCGTGTAAATATGATTACGATGGATACAAAATACt                                                                                                                            >  2:1819935/1‑150 (MQ=255)
                         aactaaaacATTTGCTGAAATTAAAGATTATTATCACAAAGGTTATGCAACAGATATCGAGATGATTGGCATTGAGGACGGCATTGTTGAATTCCATAGAAATAATGAAACAACATCGTGTAAATATGATTACGATGGATACAAAATACt                                                                                                                            >  2:1820183/1‑150 (MQ=255)
                           ctaaaacATTTGCTGAAATTAAAGATTATTATCACAAAGGTTATGCAACAGATATCGAGATGATTGGCATTGAGGACGGCATTGTTGAATTCCATAGAAATAATGAAACAACATCGTGTAAATATGATTACGATGGATACAAAATACTCa                                                                                                                          <  1:4894069/150‑1 (MQ=255)
                            taaaacATTTGCTGAAATTAAAGATTATTATCACAAAGGTTATGCAACAGATATCGAGATGATTGGCATTGAGGACGGCATTGTTGAATTCCATAGAAATAATGAAACAACATCGTGTAATTATGATTACGATGGATACAAAATACTCAc                                                                                                                         <  2:4214206/150‑1 (MQ=255)
                             aaaacATTTGCTGAAATTAAAGATTATTATCACAAAGGTTATGCAACAGATATCGAGATGATTGGCATTGAGGACGGCATTGTTGAATTCCATAGAAATAATGAAACAACATCGTGTAAATATGATTACGATGGATACAAAATACTCAcc                                                                                                                        <  2:4696127/150‑1 (MQ=255)
                             aaaacATTTGCTGAAATTAAAGATTATTATCACAAAGGTTATGCAACAGATATCGAGATGATTGGCATTGAGGACGGCATTGTTGAATTCCATAGAAATAATGAAACAACATCGTGTAAATATGATTACGATGGATACAAAATACTCAcc                                                                                                                        <  1:3246818/150‑1 (MQ=255)
                             aaaacATTTGCTGAAATTAAAGATTATTATCACAAAGGTTATGCAACAGATATCGAGATGATTGGCATTGAGGACGGCATTGTTGAATTCCATAGAAATAATGAAACAACATCGTGTAAATATGATTACGATGGATACAAAATACTCAcc                                                                                                                        <  1:3246827/150‑1 (MQ=255)
                                  aTTTGCTGAAATTAAAGATTATTATCACAAAGGTTATGCAACAGATATCGAGATGATTGGCATTGAGGACGGCATTGTTGAATTCCATAGAAATAATGAAACAACATCGTGTAATTATGATTACGATGGATACAAAATACTCACCTATaa                                                                                                                   >  1:4264527/1‑150 (MQ=255)
                                  aTTTGCTGAAATTAAAGATTATTATCACAAAGGTTATGCAACAGATATCGAGATGATTGGCATTGAGGACGGCATTGTTGAATTCCATAGAAATAATGAAACAACATCGTGTAAATATGATTACGATGGATACAAAATACTCACCTATaa                                                                                                                   >  1:3158511/1‑150 (MQ=255)
                                  aTTTGCTGAAATTAAAGATTATTATCACAAAGGTTATGCAACAGATATCGAGATGATTGGCATTGAGGACGGCATTGTTGAATTCCATAGAAATAATGAAACAACATCGTGTAAATATGATTACGATGGATACAAAATACTCACCTATaa                                                                                                                   >  1:3410550/1‑150 (MQ=255)
                                  aTTTGCTGAAATTAAAGATTATTATCACAAAGGTTATGCAACAGATATCGAGATGATTGGCATTGAGGACGGCATTGTTGAATTCCATAGAAATAATGAAACAACATCGTGTAAATATGATTACGATGGATACAAAATACTCACCTATaa                                                                                                                   >  1:3693576/1‑150 (MQ=255)
                                   tttGCTGAAATTAAAGATTATTATCACAAAGGTTATGCAACAGATATCGAGATGATTGGCATTGAGGACGGCATTGTTGAATTCCATAGAAATAATGAAACAACATCGTGTAATTATGATTACGATGGATACAAAATACTCACCTATaaa                                                                                                                  >  1:709931/1‑150 (MQ=255)
                                        tGAAATTAAAGATTATTATCACAAAGGTTATGCAACAGATATCGAGATGATTGGCATTGAGGACGGCATTGTTGAATTCCATAGAAATAATGAAACAACATCGTGTAATTATGATTACGATGGATACAAAATACTCACCTATAAATCAgg                                                                                                             >  1:400029/1‑150 (MQ=255)
                                         gAAATTAAAGATTATTATCACAAAGGTTATGCAACAGATATCGAGATGATTGGCATTGAGGACGGCATTGTTGAAATCCATAGAAATAATGAAACAACATCGTGTAATTATGATTACTATGGATACAAAATACTCACCTATAAATCAGGc                                                                                                            >  2:4193464/1‑150 (MQ=255)
                                              tAAAGATTATTATCACAAAGGTTATGCAACAGATATCGAGATGATTGGCATTGAGGACGGCATTGTTGAATTCCATAGAAATAATGAAACAACATCGTGTAAATATGATTACGATGGATACAAAATACTCACCTATAAATCAGGCAAGaa                                                                                                       <  1:1052087/150‑1 (MQ=255)
                                               aaaGATTATTATCACAAAGGTTATGCAACAGATATCGAGATGATTGGCATTGAGGACGGCATTGTTGAATTCCATAGAAATAATGAAACAACATCGTGTAATTATGATTACGATGGATACAAAATACTCACCTATAAATCAGGCAAGaaa                                                                                                      >  2:4198845/1‑150 (MQ=255)
                                               aaaGATTATTATCACAAAGGTTATGCAACAGATATCGAGATGATTGGCATTGAGGACGGCATTGTTGAATTCCATAGAAATAATGAAACAACATCGTGTAAATATGATTACGATGGATACAAAATACTCACCTATAAATCAGGCAAGaaa                                                                                                      <  1:1371666/150‑1 (MQ=255)
                                               aaaGATTATTATCACAAAGGTTATGCAACAGATATCGAGATGATTGGCATTGAGGACGGCATTGTTGAATTCCATAGAAATAATGAAACAACATCGTGTAAATATGATTACGATGGATACAAAATACTCACCTATAAATCAGGCAAGaaa                                                                                                      >  2:4042820/1‑150 (MQ=255)
                                                aaGATTATTATCACAAAGGTTATGCAACAGATATCGAGATGATTGGCATTGAGGACGGCATTGTTGAATTCCATAGAAATAATGAAACAACATCGTGTAAATATGATTACGATGGATACAAAATACTCACCTATAAATCAGGCAAGAAAg                                                                                                     >  2:1374459/1‑150 (MQ=255)
                                                aaGATTATTATCACAAAGGTTATGCAACAGATATCGAGATGATTGGCATTGAGGACGGCATTGTTGAATTCCATAGAAATAATGAAACAACATCGTGTAAATATGATTACGATGGATACAAAATACTCACCTATAAATCAGGCAAGAAAg                                                                                                     <  1:592309/150‑1 (MQ=255)
                                                aaGATTATTATCACAAAGGTTATGCAACAGATATCGAGATGATTGGCATTGAGGACGGCATTGTTGAATTCCATAGAAATAATGAAACAACATCGTGTAAATATGATTACGATGGATACAAAATACTCACCTATAAATCAGGCAAGAAAg                                                                                                     <  2:2392093/150‑1 (MQ=255)
                                                 aGATTATTATCACAAAGGTTATGCAACAGATATCGAGATGATTGGCATTGAGGACGGCATTGTTGAATTCCATAGAAATAATGAAACAACATCGTGTAAATATGATTACGATGGATACAAAATACTCACCTATAAATCAGGCAAGAAAgg                                                                                                    >  2:3972585/1‑150 (MQ=255)
                                                  gATTATTATCACAAAGGTTATGCAACAGATATCGAGATGATTGGCATTGAGGACGGCATTGTTGAATTCCATAGAAATAATGAAACAACATCGTGTAATTATGATTACGATGGATACAAAATACTCACCTATAAATCAGGCAAGAAAGGc                                                                                                   >  1:890493/1‑150 (MQ=255)
                                                     tattatCACAAAGGTTATGCAACAGATATCGAGATGATTGGCATTGAGGACGGCATTGTTGAATTCCATAGAAATAATGAAACAACATCGTGTAAATATGATTACGATGGATACAAAATACTCACCTATAAATCAGGCAAGAAAGGcgtt                                                                                                >  1:4976018/1‑150 (MQ=255)
                                                         atCACAAAGGTTATGCAACAGATATCGAGATGATTGGCATTGAGGACGGCATTGTTGAATTCCATAGAAATAATGAAACAACATCGTGTAAATATGATTACGATGGATACAAAATACTCACCTATAAATCAGGCAAGAAAGGcgttcgtt                                                                                            <  1:291286/150‑1 (MQ=255)
                                                             caAAGGTTATGCAACAGATATCGAGATGATTGGCATTGAGGACGGCATTGTTGAATTCCATAGAAATAATGAAACAACATCGTGTAATTATGATTACGATGGATACAAAATACTCACCTATAAATCAGGCACGAAAGGCGTTCGTTACtt                                                                                        >  2:2323023/1‑150 (MQ=255)
                                                             caAAGGTTATGCAACAGATATCGAGATGATTGGCATTGAGGACGGCATTGTTGAATTCCATAGAAATAATGAAACAACATCGTGTAAATATGATTACGATGGATACAAAATACTCACCTATAAATCAGGCAAGAAAGGCGTTCGTTACtt                                                                                        <  2:724321/150‑1 (MQ=255)
                                                              aaaGGTTATGCAACAGATATCGAGATGATTGGCATTGAGGACGGCATTGTTGAATTCCATAGAAATAATGAAACAACATCGTGTAAATATGATTACGATGGATACAAAATACTCACCTATAAATCAGGCAAGAAAGGCGTTCGTTACTTa                                                                                       >  2:2839820/1‑150 (MQ=255)
                                                                 ggTTATGCAACAGATATCGAGATGATTGGCATTGAGGACGGCATTGTTGAATTCCATAGAAATAATGAAACAACATCGTGTAAATATGATTACGATGGATACAAAATACTCACCTATAAATCAGGCAAGAAAGGCGTTCGTTACTTATTc                                                                                    <  2:1727147/150‑1 (MQ=255)
                                                                  gtaaTGCAACAGATATCGAGATGATTGGCATTGAGGACGGCATTGTTGAATTCCATAGAAATAATGAAACAACATCGTGTAAATATGATTACGATGGATACAAAATACTCACCTATAAATCAGGCAAGAAAGGCGTTCGTTACTTATTCg                                                                                   <  1:1748591/147‑1 (MQ=255)
                                                                  gTTATGCAACAGATATCGAGATGATTGGCATTGAGGACGGCATTGTTGAATTCCATAGAAATAATGAAACAACATCGTGTAAATATGATTACGATGGATACAAAATACTCACCTATAAATCAGGCAAGAAAGGCGTTCGTTACTTATTCg                                                                                   >  2:1593939/1‑150 (MQ=255)
                                                                    tATGCAACAGATATCGAGATGATTGGCATTGAGGACGGCATTGTTGAATTCCATAGAAATAATGAAACAACATCGTGTAATTATGATTACGATGGATACAAAATACTCACCTATAAATCAGGCAAGAAAGGCGTTCGTTACTTATTCGaa                                                                                 >  1:4650328/1‑150 (MQ=255)
                                                                       gCAACAGATATCGAGATGATTGGCATTGAGGACGGCATTGTTGAATTCCATAGAAATAATGAAACAACATCGTGTAAATATGATTACGATGGATACAAAATACTCACCTATAAATCAGGCAAGAAAGGCGTTCGTTACTTATTCGAATGt                                                                              >  2:3232849/1‑150 (MQ=255)
                                                                       gCAACAGATATCGAGATGATTGGCATTGAGGACGGCATTGTTGAATTCCATAGAAATAATGAAACAACATCGTGTAAATATGATTACGATGGATACAAAATACTCACCTATAAATCAGGCAAGAAAGGCGTTCGTTACTTATTCGAATGt                                                                              >  2:340444/1‑150 (MQ=255)
                                                                        cAACAGATATCGAGATGATTGGCATTGAGGACGGCATTGTTGAATTCCATAGAAATAATGAAACAACATCGTGTAAATATGATTACGATGGATACAAAATACTCACCTATAAATCAGGCAAGAAAGGCGTTCGTTACTTATTCGAATGTa                                                                             >  2:2378475/1‑150 (MQ=255)
                                                                        cAACAGATATCGAGATGATTGGCATTGAGGACGGCATTGTTGAATTCCATAGAAATAATGAAACAACATCGTGTAAATATGATTACGATGGATACAAAATACTCACCTATAAATCAGGCAAGAAAGGCGTTCGTTACTTATTCGAATGTa                                                                             >  1:1837485/1‑150 (MQ=255)
                                                                         aaCAGATATCGAGATGATTGGCATTGAGGACGGCATTGTTGAATTCCATAGAAATAATGAAACAACATCGTGTAAATATGATTACGATGGATACAAAATACTCACCTATAAATCAGGCAAGAAAGGCGTTCGTTACTTATTCGAATGTaa                                                                            <  1:413894/150‑1 (MQ=255)
                                                                         aaCAGATATCGAGATGATTGGCATTGAGGACGGCATTGTTGAATTCCATAGAAATAATGAAACAACATCGTGTAAATATGATTACGATGGATACAAAATACTCACCTATAAATCAGGCAAGAAAGGCGTTCGTTACTTATTCGAATGTaa                                                                            <  2:2027378/150‑1 (MQ=255)
                                                                            aGATATCGAGATGATTGGCATTGAGGACGGCATTGTTGAATTCCATAGAAATAATGAAACAACATCGTGTAAATATGATTACGATGGATACAAAATACTCACCTATAAATCAGGCAAGAAAGGCGTTCGTTACTTATTCGAATGTAAAGa                                                                         >  2:3024657/1‑150 (MQ=255)
                                                                             gATATCGAGATGATTGGCATTGAGGACGGCATTGTTGAATTCCATAGAAATAATGAAACAACATCGTGTAATTATGATTACGATGGATACAAAATACTCACCTATAAATCAGGCAAGAAAGGCGTTCGTTACTTATTCGAATGTAAAGAt                                                                        >  1:4138075/1‑150 (MQ=255)
                                                                             gATATCGAGATGATTGGCATTGAGGACGGCATTGTTGAATTCCATAGAAATAATGAAACAACATCGTGTAAATATGATTACGATGGATACAAAATACTCGCCTATAAATCAGGCAAGAAAGGCGTTCGTTACTTATTCGAATGTAAAGAt                                                                        >  1:2955724/1‑150 (MQ=255)
                                                                               tatCGAGATGATTGGCATTGAGGACGGCATTGTTGAATTCCATAGAAATAATGAAACAACATCGTGTAATTATGATTACGATGGATACAAAATACTCACCTATAAATCAGGCAAGAAAGGCGTTCGTTACTTATTCGAATGTAAAGATcc                                                                      >  1:2767628/1‑150 (MQ=255)
                                                                               tatCGAGATGATTGGCATTGAGGACGGCATTGTTGAATTCCATAGAAATAATGAAACAACATCGTGTAAATATGATTACGATGGATACAAAATACTCACCTATAAATCAGGCAAGAAAGGCGTTCGTTACTTATTCGAATGTAAAGATcc                                                                      <  2:2717241/150‑1 (MQ=255)
                                                                               tatCGAGATGATTGGCATTGAGGACGGCATTGTTGAATTCCATAGAAATAATGAAACAACATCGTGTAAATATGATTACGATGGATACAAAATACTCACCTATAAATCAGGCAAGAAAGGCGTTCGTTACTTATTCGAATGTAAAGATcc                                                                      >  2:1519727/1‑150 (MQ=255)
                                                                                atCGAGATGATTGGCATTGAGGACGGCATTGTTGAATTCCATAGAAATAATGAAACAACATCGTGTAAATATGATTACGATGGATACAAAATACTCACCTATAAATCAGGCAAGAAAGGCGTTCGTTACTTATTCGAATGTAAAGATCCt                                                                     >  1:4276649/1‑150 (MQ=255)
                                                                                atCGAGATGATTGGCATTGAGGACGGCATTGTTGAATTCCATAGAAATAATGAAACAACATCGTGTAAATATGATTACGATGGATACAAAATACTCACCTATAAATCAGGCAAGAAAGGCGTTCGTTACTTATTCGAATGTAAAGATCCt                                                                     >  2:3772140/1‑150 (MQ=255)
                                                                                   gagaTGATTGGCATTGAGGACGGCATTGTTGAATTCCATAGAAATAATGAAACAACATCGTGTAAATATGATTACGATGGATACAAAATACTCACCTATAAATCAGGCAAGAAAGGCGTTCGTTACTTATTCGAATGTAAAGATCCTGaa                                                                  >  2:1979097/1‑150 (MQ=255)
                                                                                   gagaTGATTGGCATTGAGGACGGCATTGTTGAATTCCATAGAAATAATGAAACAACATCGTGTAAATATGATTACGATGGATACAAAATACTCACCTATAAATCAGGCAAGAAAGGCGTTCGTTACTTATTCGAATGTAAAGATCCTGaa                                                                  <  2:2534331/150‑1 (MQ=255)
                                                                                   gagaTGATTGGCATTGAGGACGGCATTGTTGAATTCCATAGAAATAATGAAACAACATCGTGTAAATATGATTACGATGGATACAAAATACTCACCTATAAATCAGGCAAGAAAGGCGATCGTTACTTATTCGAATGTAAAGATCCTGaa                                                                  >  1:1810532/1‑150 (MQ=255)
                                                                                     gatgatTGGCATTGAGGACGGCATTGTTGAATTCCATAGAAATAATGAAACAACATCGTGTAAATATGATTACGATGGATACAAAATACTCACCTATAAATCAGGCAAGAAAGGCGTTCGTTACTTATTCGAATGTAAAGATCCTGaaag                                                                <  1:4195002/150‑1 (MQ=255)
                                                                                       tgatTGGCATTGAGGACGGCATTGTTGAATTCCATAGAAATAATGAAACAACATCGTGTAAATATGATTACGATGGATACAAAATACTCACCTATAAATCAGGCAAGAAAGGCGTTCGTTACTTATTCGAATGTAAAGATCCTGaaagca                                                              <  2:2690420/150‑1 (MQ=255)
                                                                                         atTGGCATTGAGGACGGCATTGTTGAATTCCATAGAAATAATGAAACAACATCGTGTAATTATGATTACGATGGATACAAAATACTCACCTATAAATCAGGCAAGAAAGGCGTTCGTTACTTATTCGAATGTAAAGATCCTGaaagcaaa                                                            >  1:1176721/1‑150 (MQ=255)
                                                                                         atTGGCATTGAGGACGGCATTGTTGAATTCCATAGAAATAATGAAACAACATCGTGTAAATATGATTACGATGGATACAAAATACTCACCTATAAATCAGGCAAGAAAGGCGTTCGTTACTTATTCGAATGTAAAGATCCTGaaagcaaa                                                            >  2:4987956/1‑150 (MQ=255)
                                                                                          ttGGCATTGAGGACGGCATTGTTGAATTCCATAGAAATAATGAAACAACATCGTGTAAATATGATTACGATGGATACAAAATACTCACCTATAAATCAGGCAAGAAAGGCGTTCGTTACTTATTCGAATGTAAAGATCCTGaaagcaaag                                                           >  1:3621868/1‑150 (MQ=255)
                                                                                               aTTGAGGACGGCATTGTTGAATTCCATAGAAATAATGAAACAACATCGTGTAAATATGATTACGATGGATACAAAATACTCACCTATAAATCAGGCAAGAAAGGCGTTCGTTACTTATTCGAATGTAAAGATCCTGAAAGCAAAGCCCCt                                                      >  2:311882/1‑150 (MQ=255)
                                                                                                 tGAGGACGGCATTGTTGAATTCCATAGAAATAATGAAACAACATCGTGTAAATATGATTACGATGGATACAAAATACTCACCTATAAATCAGGCAAGAAAGGCGTTCGTTACTTATTCGAATGTAAAGATCCTGAAAGCAAAGCCCCTaa                                                    <  1:1611013/150‑1 (MQ=255)
                                                                                                  gAGGACGGCATTGTTGAATTCCATAGAAATAATGAAACAACATCGTGTAAATATGATTACGATGGATACAAAATACTCACCTATAAATCAGGCAAGAAAGGCGTTCGTTACTTATTCGAATGTAAAGATCCTGAAAGCAAAGCCCCTaaa                                                   >  2:50332/1‑150 (MQ=255)
                                                                                                   aGGACGGCATTGTTGAATTCCATAGAAATAATGAAACAACATCGTGTAAATATGATTACGATGGATACAAAATACTCACCTATAAATCAGGCAAGAAAGGCGTTCGTTACTTATTCGAATGTAAAGATCCTGAAAGCAAAGCCCCTAAat                                                  <  2:3315408/150‑1 (MQ=255)
                                                                                                    ggACGGCATTGTTGAATTCCATAGAAATAATGAAACAACATCGTGTAAATATGATTACGATGGATACAAAATACTCACCTATAAATCAGGCAAGAAAGGCGTTCGTTACTTATTCGAATGTAAAGATCCTGAAAGCAAAGCCCCTAAata                                                 >  1:3741401/1‑150 (MQ=255)
                                                                                                     gACGGCATTGTTGAATTCCATAGAAATAATGAAACAACATCGTGTAATTATGATTACGATGGATACAAAATACTCACCTATAAATCAGGCAAGAAAGGCGTTCGTTACTTATTCGAATGTAAAGATCCTGAAAGCAAAGCCCCTAAatat                                                <  1:3251134/150‑1 (MQ=255)
                                                                                                     gACGGCATTGTTGAATTCCATAGAAATAATGAAACAACATCGTGTAAATATGATTACGATGGATACAAAATACTCACCTATAAATCAGGCAAGAAAGGCGTTCGTTACTTATTCGAATGTAATGATCCTGAAAGCAAAGCCCCTAAatat                                                >  1:1143686/1‑150 (MQ=255)
                                                                                                     gACGGCATTGTTGAATTCCATAGAAATAATGAAACAACATCGTGTAAATATGATTACGATGGATACAAAATACTCACCTATAAATCAGGCAAGAAAGGCGTTCGTTACTTATTCGAATGTAAAGATCCTGAAAGCAAAGCCCCTAAatat                                                >  2:4321133/1‑150 (MQ=255)
                                                                                                      aCGGCATTGTTGAATTCCATAGAAATAATGAAACAACATCGTGTAAATATGATTACGATGGATACAAAATACTCACCTATAAATCAGGCAAGAAAGGCGTTCGTTACTTATTCGAATGTAAAGATCCTGAAAGCAAAGCCCCTAAatata                                               <  2:4867187/150‑1 (MQ=255)
                                                                                                           aTTGTTGAATTCCATAGAAATAATGAAACAACATCGTGTAAATATGATTACGATGGATACAAAATACTCACCTATAAATCAGGCACGAAAGGCGTTCGTTACTTATTCGAATGTAAAGATCCTGAAAGCAAAGCCCCTAAATATATACaa                                          >  2:4510973/1‑150 (MQ=255)
                                                                                                           aTTGTTGAATTCCATAGAAATAATGAAACAACATCGTGTAAATATGATTACGATGGATACAAAATACTCACCTATAAATCAGGCAAGAAAGGCGTTCGTTACTTATTCGAATGTAAAGATCCTGAAAGCAAAGCCCCTAAATATATACaa                                          >  1:3835138/1‑150 (MQ=255)
                                                                                                           aTTGTTGAATTCCATAGAAATAATGAAACAACATCGTGTAAATATGATTACGATGGATACAAAATACTCACCTATAAATCAGGCAAGAAAGGCGTTCGTTACTTATTCGAATGTAAAGATCCTGAAAGCAAAGCACCTAAATATATACaa                                          >  1:238658/1‑150 (MQ=255)
                                                                                                             tgttgAATTCCATAGAAATAATGAAACAACATCGTGTAATTATGATTACGATGGATACAAAATACTCACCTATAAATCAGGCAAGAAAGGCGTTCGTTACTTATTCGAATGTAAAGATCCTGAAAGCAAAGCCCCTAAATATATACAAtt                                        <  1:1916689/150‑1 (MQ=255)
                                                                                                                tgAATTCAATAGAAATAATGAAACAACATCGTGTAAATATGATTACGATGGATACAAAATACTCACCTATAAATCAGGCAAGAAAGGCGTTCGTTACTTATTCGAATGTAAAGATCCTGAAAGCAAAGCCCCTAAATATATACAATTTAg                                     <  1:4238280/150‑1 (MQ=255)
                                                                                                                    ttCCATAGAAATAATGAAACAACATCGTGTAAATATGATTACGATGGATACAAAATACTCACCTATAAATCAGGCAAGAAAGGCGTTCGTTACTTATTCGAATGTAAAGATCCTGAAAGCAAAGCCCCTAAATATATACAATTTAGCGAt                                 <  1:301354/150‑1 (MQ=255)
                                                                                                                      ccATAGAAATAATGAAACAACATCGTGTAAATATGATTACGATGGATACAAAATACTCACCTATAAATCAGGCAAGAAAGGCGTTCGTTACTTATTCGAATGTAAAGATCCTGAAAGCAAAGCCCCTAAATATATACAATTTAGAGATCa                               >  1:4951037/1‑150 (MQ=255)
                                                                                                                         tAGAAATAATGAAACAACATCGTGTAAATATGATTACGATGGATACAAAATACTCACCTATAAATCAGGCAAGAAAGGCGTTCGTTACTTATTCGAATGTAAAGATCCTGAAAGCAAAGCCCCTAAATATATACAATTTAGCGATCatat                            >  2:1623924/1‑150 (MQ=255)
                                                                                                                            aaaTAATGAAACAACATCGTGTAAATATGATTACGATGGATACAAAATACTCACCTATAAATCAGGCAAGAAAGGCGTTCGTTACTTATTCGAATGTAAAGATCCTGAAAGCAAAGCCCCTAAATATATACAATTTAGCGATCATATAAt                         >  2:4072544/1‑150 (MQ=255)
                                                                                                                            aaaTAATGAAACAACATCGTGTAAATATGATTACGATGGATACAAAATACTCACCTATAAATCAGGCAAGAAAGGCGTTCGTTACTTATTCGAATGTAAAGATCCTGAAAGCAAAGCCCCTAAATATATACAATTTAGCGATCATATAAt                         <  1:2442903/150‑1 (MQ=255)
                                                                                                                                 atGAAACAACATCGTGTAAATATGGTTACGATGGATACAAAATACTCACCTATAAATCAGGCAAGAAAGGCGTTCGTTACTTATTCGAATGTAAAGATCCTGAAAGCAAAGCCCCTAAATATATACAATTTAGCGATCATATAATTGcac                    >  1:4071955/1‑150 (MQ=255)
                                                                                                                                 atGAAACAACATCGTGTAAATATGATTACGATGGATACAAAATACTCACCTATAAATCAGGCAAGAAAGGCGTTCGTTACTTATTCGAATGTAAAGATCCTGAAAGCAAAGCCCCTAAATATATACAATTTAGCGATCATATAATTGcac                    >  2:601291/1‑150 (MQ=255)
                                                                                                                                  tGAAACAACATCGTGTAAATATGATTACGATGGATACAAAATACTCACCTATAAATCAGGCAAGAAAGGCGTTCGTTACTTATTCGAATGTAAAGATCCTGAAAGCAAAGCCCCTAAATATATACAATTTAGCGATCATATAATTGcacc                   >  1:4517736/1‑150 (MQ=255)
                                                                                                                                  tGAAACAACATCGTGTAAATATGATTACGATGGATACAAAATACTCACCTATAAATCAGGCAAGAAAGGCGTTCGTTACTTATTCGAATGTAAAGATCCTGAAAGCAAAGCCCCTAAATATATACAATTTAGCGATCATATAATTGcacc                   <  2:1528988/150‑1 (MQ=255)
                                                                                                                                    aaaCAACATCGTGTAAATATGATTACGATGGATACAAAATACTCACCTATAAATCAGGCAAGAAAGGCGTTCGTTACTTATTCGAATGTAAAGATCCTGAAAGCAAAGCCCCTAAATATATACAATTTAGCGATCATATAATTGcaccac                 >  2:3454603/1‑150 (MQ=255)
                                                                                                                                        aacaTCGTGTAATTATGATTACGATGGATACAAAATACTCACCTATAAATCAGGCAAGAAAGGCGTTCGTTACTTATTCGAATGTAAAGATCCTGAAAGCAAAGCCCCTAAATATATACAATTTAGCGATCATATAATTGCACCACGaaa             <  2:3325948/150‑1 (MQ=255)
                                                                                                                                          caTCGTGTAATTATGATTACGATGGATACAAAATACTCACCTATAAATCAGGCAAGAAAGGCGTTCGTTACTTATTCGAATGTAAAGATCCTGAAAGCAAAGCCCCTAAATATATACAATTTAGCGATCATATAATTGCACCACGAAAat           >  2:116239/1‑150 (MQ=255)
                                                                                                                                          caTCGTGTAAATATGATTACGATGGATACAAAATACTCACCTATAAATCAGGCAAGAAAGGCGTTCGTTACTTATTCGAATGTAAAGATCCTGAAAGCAAAGCCCCTAAATATATACAATTTAGCGATCATATAATTGCACCACGAAAat           >  1:1985364/1‑150 (MQ=255)
                                                                                                                                              gtgtAAATATGATTACGATGGATACAAAATACTCACCTATAAATCAGGCAAGAAAGGCGTTCGTTACTTATTCGAATGTAAAGATCCTGAAAGCAAAGCCCCTAAATATATACAATTTAGCGATCATATAATTGCACCACGAAAatcatc       <  1:220343/150‑1 (MQ=255)
                                                                                                                                               tgtAAATATGATTACGATGGATACAAAATACTCACCTATAAATCAGGCAAGAAAGGCGTTCGTTACTTATTCGAATGTAAAGATCCTGAAAGCAAAGCCCCTAAATATATACAATTTAGCGATCATATAATTGCACCACGAAAATCAtct      <  1:772094/150‑1 (MQ=255)
                                                                                                                                                 tAAATATGATTACGATGGATACAAAATACTCACCTATAAATCAGGCAAGAAAGGCGTTCGTTACTTATTCGAATGTAAAGATCCTGAAAGCAAAGCCCCTAAATATATACAATTTAGCGATCATATAATTGCACCACGAAAATCATCTCa    >  2:2491261/1‑150 (MQ=255)
                                                                                                                                                 tAAATATGATTACGATGGATACAAAATACTCACCTATAAATCAGGCAAGAAAGGCGTTCGTTACTTATTCGAATGTAAAGATCCTGAAAGCAAAGCCCCTAAATATATACAATTTAGCGATCATATAATTGCACCACGAAAATCATCTCa    >  2:2490919/1‑150 (MQ=255)
                                                                                                                                                   aaTATGATTACGATGGATACAAAATACTCACCTATAAATCAGGCAAGAAAGGCGTTCGTTACTTATTCGAATGTAAAGATCCTGAAAGCAAAGCCCCTAAATATATACAATTTAGCGATCATATAATTGCACCACGAAAATCATCTTAtt  <  2:112275/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
TTCAGAAGAAAGCGGATGCAGATAAAACTAAAACATTTGCTGAAATTAAAGATTATTATCACAAAGGTTATGCAACAGATATCGAGATGATTGGCATTGAGGACGGCATTGTTGAATTCCATAGAAATAATGAAACAACATCGTGTAAATATGATTACGATGGATACAAAATACTCACCTATAAATCAGGCAAGAAAGGCGTTCGTTACTTATTCGAATGTAAAGATCCTGAAAGCAAAGCCCCTAAATATATACAATTTAGCGATCATATAATTGCACCACGAAAATCATCTCATT  >  CP009273/2035088‑2035384

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A