Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F309 I0 R1 1697 197.2 6627756 95.7% 6342762 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 406,075 C→A 100% P159Q (CCG→CAG)  mak → manno(fructo)kinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273406,0750CA100.0% 586.8 / NA 175P159Q (CCG→CAG) makmanno(fructo)kinase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (65/110);  total (65/110)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 175 total aligned reads displayed.

GTTTCAGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATCGGCACGGGATGCGGCGCGGGCGTGGCATTCAATGGGCGGGCGCATATCGGCGGCAATGGCACGGCAGGTGAGTGGGGACACAATCCGCTACCGTGGATGGACGAAGACGAACTGCGTTATCGCGAGGAAGTCCCTTGTTATTGCGGTAAACAAGGTTGTATTGAAACCTTTATTTCGGGCACGGGATTCGCGATGGATTATCGTCGTTTGAGCGGACATGCGCTGAAAGG  >  CP009273/405930‑406219
                                                                                                                                                 |                                                                                                                                                
gTTTCAGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATCGGCACGGGATGCGGCGCGGGCGTGGCATTCAATGGGCGGGCGCATATCGGCGGCAATGGCACGGCAGGTGAGTGGGGACACAATCAGc                                                                                                                                                >  1:619422/1‑148 (MQ=255)
   tCAGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATCGGCACGGGATGCGGCGCGGGCGTGGCATTCAATGGGCGGGCGCATATCGGCGGCAATGGCACGGCAGGTGAGTGGGGACACAATCAGCTAc                                                                                                                                             <  2:1791830/148‑1 (MQ=255)
      gAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATCGGCACGGGATGCGGCGCGGGCGTGGCATTCAATGGGCGGGCGCATATCGGCGGCAATGGCACGGCAGGTGAGTGGGGACACAATCAGCTACCGt                                                                                                                                          <  2:1576614/148‑1 (MQ=255)
      gAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATCGGCACGGGATGCGGCGCGGGCGTGGCATTCAATGGGCGGGCGCATATCGGCGGCAATGGCACGGCAGGTGAGTGGGGACACAATCAGCTACCGt                                                                                                                                          >  1:1294143/1‑148 (MQ=255)
         gCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATCGGCACGGGATGCGGCGCGGGCGTGGCATTCAATGGGCGGGCGCATATCGGCGGCAATGGCACGGCAGGTGAGTGGGGACACAATCAGCTACCGtgga                                                                                                                                       >  1:3205033/1‑148 (MQ=255)
         gCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATCGGCACGGGATGCGGCGCGGGCGTGGCATTCAATGGGCGGGCGCATATCGGCGGCAATGGCACGGCAGGTGAGTGGGGACACAATCAGCTACCGtgga                                                                                                                                       >  2:2610745/1‑148 (MQ=255)
         gCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATCGGCACGGGATGCGGCGCGGGCGTGGCATTCAATGGGCGGGCGCATATCGGCGGCAATGGCACGGCAGGTGAGTGGGGACACAATCAGCTACCGtgga                                                                                                                                       >  1:3205031/1‑148 (MQ=255)
          cAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATCGGCACGGGATGCGGCGCGGGCGTGGCATTCAATGGGCGGGCGCATATCGGCGGCAATGGCACGGCAGGTGAGTGGGGACACAATCAGCTACCGtggat                                                                                                                                      >  1:2473595/1‑148 (MQ=255)
             tAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATCGGCACGGGATGCGGCGCGGGCGTGGCATTCAATGGGCGGGCGCATATCGGCGGCAATGGCACGGCAGGTGAGTGGGGACACAATCAGCTACCGtggatgga                                                                                                                                   <  1:1450983/148‑1 (MQ=255)
             tAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATCGGCACGGGATGCGGCGCGGGCGTGGCATTCAATGGGCGGGCGCATATCGGCGGCAATGGCACGGCAGGTGAGTGGGGACACAATCAGCTACCGtggatgga                                                                                                                                   >  1:2845952/1‑148 (MQ=255)
             tAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATCGGCACGGGATGCGGCGCGGGCGTGGCATTCAATGGGCGGGCGCATATCGGCGGCAATGGCACGGCAGGTGAGTGGGGACACAATCAGCTACCGtggatgga                                                                                                                                   <  1:3096516/148‑1 (MQ=255)
                aTGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATCGGCACGGGATGCGGCGCGGGCGTGGCATTCAATGGGCGGGCGCATATCGGCGGCAATGGCACGGCAGGTGAGTGGGGACACAATCAGCTACCGTGGATGgacga                                                                                                                                >  1:2171673/1‑148 (MQ=255)
                 tGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATCGGCACGGGATGCGGCGCGGGCGTGGCATTCAATGGGCGGGCGCATATCGGCGGCAATGGCACGGCAGGTGAGTGGGGACACAATCAGCTACCGTGGATGgacgaa                                                                                                                               <  1:478570/148‑1 (MQ=255)
                  ggCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATCGGCACGGGATGCGGCGCGGGCGTGGCATTCAATGGGCGGGCGCATATCGGCGGCAATGGCACGGCAGGTGAGTGGGGACACAATCAGCTACCGTGGATGgacgaag                                                                                                                              <  2:1869087/148‑1 (MQ=255)
                   gcgcGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATCGGCACGGGATGCGGCGCGGGCGTGGCATTCAATGGGCGGGCGCATATCGGCGGCAATGGCACGGCAGGTGAGTGGGGACACAATCAGCTACCGTGGATGgacgaaga                                                                                                                             <  1:953868/148‑1 (MQ=255)
                             gggAGCGCAGACGGTATTTGCCGTGATTATCGGCACGGGATGCGGCGCGGGCGTGGCATTCAATGGGCGGGCGCATATCGGCGGCAATGGCACGGCAGGTGAGTGGGGACACAATCAGCTACCGTGGATGGACGAAGACGAACTGCGt                                                                                                                   <  2:3209797/148‑1 (MQ=255)
                               gAGCGCAGACGGTATTTGCCGTGATTATCGGCACGGGATGCGGCGCGGGCGTGGCATTCAATGGGCGGGCGCATATCGGCGGCAATGGCACGGCAGGTGAGTGGGGACACAATCAGCTACCGTGGATGGACGAAGACGAACTGCGTTa                                                                                                                 <  1:2288701/148‑1 (MQ=255)
                               gAGCGCAGACGGTATTTGCCGTGATTATCGGCACGGGATGCGGCGCGGGCGTGGCATTCAATGGGCGGGCGCATATCGGCGGCAATGGCACGGCAGGTGAGTGGGGACACAATCAGCTACCGTGGATGGACGAAGACGAACTGCGTTa                                                                                                                 <  1:2288645/148‑1 (MQ=255)
                                  cgcAGACGGTATTTGCCGTGATTATCGGCACGGGATGCGGCGCGGGCGTGGCATTCAATGGGCGGGCGCATATCGGCGGCAATGGCACGGCAGGTGAGTGGGGACACAATCAGCTACCGTGGATGGACGAAGACGAACTGCGTTATcg                                                                                                              >  1:1611472/1‑148 (MQ=255)
                                    cAGACGGTATTTGCCGTGATTATCGGCACGGGATGCGGCGCGGGCGTGGCATTCAATGGGCGGGCGCATATCGGCGGCAATGGCACGGCAGGTGAGTGGGGACACAATCAGCTACCGTGGATGGACGAAGACGAACTGCGTTATcgcg                                                                                                            <  1:556039/148‑1 (MQ=255)
                                     aGACGGTATTTGCCGTGATTATCGGCACGGGATGCGGCGCGGGCGTGGCATTCAATGGGCGGGCGCATATCGGCGGCAATGGCACGGCAGGTGAGTGGGGACACAATCAGCTACCGTGGATGGACGAAGACGAACTGCGTTATCGCGa                                                                                                           <  2:1303652/148‑1 (MQ=255)
                                      gACGGTATTTGCCGTGATTATCGGCACGGGATGCGGCGCGGGCGTGGCATTCAATGGGCGGGCGCATATCGGCGGCAATGGCACGGCAGGTGAGTGGGGACACAATCAGCTACCGTGGATGGACGAAGACGAACTGCGTTATCGCGAg                                                                                                          >  1:231553/1‑148 (MQ=255)
                                      gACGGTATTTGCCGTGATTATCGGCACGGGATGCGGCGCGGGCGTGGCATTCAATGGGCGGGCGCATATCGGCGGCAATGGCACGGCAGGTGAGTGGGGACACAATCAGCTACCGTGGATGGACGAAGACGAACTGCGTTATCGCGAg                                                                                                          >  2:2277564/1‑148 (MQ=255)
                                         ggTATTTGCCGTGATTATCGGCACGGGATGCGGCGCGGGCGTGGCATTCAATGGGCGGGCGCATATCGGCGGCAATGGCACGGCAGGTGAGTGGGGACACAATCAGCTACCGTGGATGGACGAAGACGAACTGCGTTATCGCGAGGaa                                                                                                       <  2:606850/148‑1 (MQ=255)
                                          gTATTTGCCGTGATTATCGGCACGGGATGCGGCGCGGGCGTGGCATTCAATGGGCGGGCGCATATCGGCGGCAATGGCACGGCAGGTGAGTGGGGACACAATCAGCTACCGTGGATGGACGAAGACGAACTGCGTTATCGCGAGGAAg                                                                                                      <  2:345480/148‑1 (MQ=255)
                                           tATTTGCCGTGATTATCGGCACGGGATGCGGCGCGGGCGTGGCATTCAATGGGCGGGCGCATATCGGCGGCAATGGCACGGCAGGTGAGTGGGGACACAATCAGCTACCGTGGATGGACGAAGACGAACTGCGTTATCGCGAGGAAGt                                                                                                     <  1:2469581/148‑1 (MQ=255)
                                           tATTTGCCGTGATTATCGGCACGGGATGCGGCGCGGGCGTGGCATTCAATGGGCGGGCGCATATCGGCGGCAATGGCACGGCAGGTGAGTGGGGACACAATCAGCTACCGTGGATGGACGAAGACGAACTGCGTTATCGCGAGGAAGt                                                                                                     <  2:1680436/148‑1 (MQ=255)
                                            aTTTGCCGTGATTATCGGCACGGGATGCGGCGCGGGCGTGGCATTCAATGGGCGGGCGCATATCGGCGGCAATGGCACGGCAGGTGAGTGGGGACACAATCAGCTACCGTGGATGGACGAAGACGAACTGCGTTATCGCGAGGAAGTc                                                                                                    <  1:2191825/148‑1 (MQ=255)
                                             tttGCCGTGATTATCGGCACGGGATGCGGCGCGGGCGTGGCATTCAATGGGCGGGCGCATATCGGCGGCAATGGCACGGCAGGTGAGTGGGGACACAATCAGCTACCGTGGATGGACGAAGACGAACTGCGTTATCGCGAGGAAGTcc                                                                                                   >  2:3196716/1‑148 (MQ=255)
                                               tGCCGTGATTATCGGCACGGGATGCGGCGCGGGCGTGGCATTCAATGGGCGGGCGCATATCGGCGGCAATGGCACGGCAGGTGAGTGGGGACACAATCAGCTACCGTGGATGGACGAAGACGAACTGCGTTATCGCGAGGAAGTCCCt                                                                                                 <  1:328009/148‑1 (MQ=255)
                                                 ccGTGATTATCGGCACGGGATGCGGCGCGGGCGTGGCATTCAATGGGCGGGCGCATATCGGCGGCAATGGCACGGCAGGTGAGTGGGGACACAATCAGCTACCGTGGATGGACGAAGACGAACTGCGTTATCGCGAGGAAGTCCCTTg                                                                                               <  2:765472/148‑1 (MQ=255)
                                                  cGTGATTATCGGCACGGGATGCGGCGCGGGCGTGGCATTCAATGGGCGGGCGCATATCGGCGGCAATGGCACGGCAGGTGAGTGGGGACACAATCAGCTACCGTGGATGGACGAAGACGAACTGCGTTATCGCGAGGAAGTCCCTTGt                                                                                              <  1:1567784/148‑1 (MQ=255)
                                                    tGATTATCGGCACGGGTTGCGGCGCGGGCGTGGCATTAAATGGTCGTGCGCATATCGGCGGCATTGGAACTGCAGGTGAGTGGGGACTCAATCAGCTACATTGGATGGACGAAGACGAACTGCGTTATCGAGAGGAAGTCCCTTGTTa                                                                                            <  1:819033/148‑1 (MQ=255)
                                                     gATTATCGGCACGGGATGCGGCGCGGGCGTGGCATTCAATGGGCGGGCGCATATCGGCGGCAATGGCACGGCAGGTGAGTGGGGACACAATCAGCTACCGTGGATGGTCGAAGACGAACTGCGTTATCGCGAGGAAGTCCCTTGTTAt                                                                                           <  2:1328841/148‑1 (MQ=255)
                                                     gATTATCGGCACGGGATGCGGCGCGGGCGTGGCATTCAATGGGCGGGCGCATATCGGCGGCAATGGCACGGCAGGTGAGTGGGGACACAATCAGCTACCGTGGATGGACGAAGACGAACTGCGTTATCGCGAGGAAGTCCCTTGTTAt                                                                                           <  2:2975440/148‑1 (MQ=255)
                                                     gATTATCGGCACGGGATGCGGCGCGGGCGTGGCATTCAATGGGCGGGCGCATATCGGCGGCAATGGCACGGCAGGTGAGTGGGGACACAATCAGCTACCGTGGATGGACGAAGACGAACTGCGTTATCGCGAGGAAGTCCCTTGTTAt                                                                                           <  2:1542561/148‑1 (MQ=255)
                                                     gATTATCGGCACGGGATGCGGCGCGGGCGTGGCATTCAATGGGCGGGCGCATATCGGCGGCAATGGCACGGCAGGTGAGTGGGGACACAATCAGCTACCGTGGATGGACGAAGACGAACTGCGTTATCGCGAGGAAGTCCCTTGTTAt                                                                                           <  2:2156979/148‑1 (MQ=255)
                                                     gATTATCGGCACGGGATGCGGCGCGGGCGTGGCATTCAATGGGCGGGCGCATATCGGCGGCAATGGCACGGCAGGTGAGTGGGGACACAATCAGCTACCGTGGATGGACGAAGACGAACTGCGTTATCGCGAGGAAGTCCCTTGTTAt                                                                                           >  1:2106742/1‑148 (MQ=255)
                                                     gATTATCGGCACGGGATGCGGCGCGGGCGTGGCATTCAATGGGCGGGCGCATATCGGCGGCAATGGCACGGCAGGTGAGTGGGGACACAATCAGCTACCGTGGATGGACGAAGACGAACTGCGTTATCGCGAGGAAGTCCCTTGTTAt                                                                                           >  1:2106473/1‑148 (MQ=255)
                                                       ttATCGGCACGGGATGCGGCGCGGGCGTGGCATTCAATGGGCGGGCGCATATCGGCGGCAATGGCACGGCAGGTGAGTGGGGACACAATCAGCTACCGTGGATGGACGAAGACGAACTGCGTTATCGCGAGGAAGTCCCTTGTTATTg                                                                                         <  2:670697/148‑1 (MQ=255)
                                                         aTCGGCACGGGATGCGGCGCGGGCGTGGCATTCAATGGGCGGGCGCATATCGGCGGCAATGGCACGGCAGGTGAGTGGGGACACAATCAGCTACCGTGGATGGACGAAGACGAACTGCGTTATCGCGAGGAAGTCCCTTGTTATTGCg                                                                                       >  2:1157382/1‑148 (MQ=255)
                                                           cGGCACGGGATGCGGCGCGGGCGTGGCATTCAATGGGCGGGCGCATATCGGCGGCAATGGCACGGCAGGTGAGTGGGGACACAATCAGCTACCGTGGATGGACGAAGACGAACTGCGTTATCGCGAGGAAGTCCCTTGTTATTGCGGt                                                                                     >  2:2221792/1‑148 (MQ=255)
                                                           cGGCACGGGATGCGGCGCGGGCGTGGCATTCAATGGGCGGGCGCATATCGGCGGCAATGGCACGGCAGGTGAGTGGGGACACAATCAGCTACCGTGGATGGACGAAGACGAACTGCGTTATCGCGAGGAAGTCCCTTGTTATTGCGGt                                                                                     <  1:185027/148‑1 (MQ=255)
                                                             gCACGGGATGCGGCGCGGGCGTGGCATTCAATGGGCGGGCGCATATCGGCGGCAATGGCACGGCAGGTGAGTGGGGACACAATCAGCTACCGTGGATGGACGAAGACGAACTGCGTTATCGCGAGGAAGTCCCTTGTTATTGCGGTaa                                                                                   <  2:3183643/148‑1 (MQ=255)
                                                             gCACGGGATGCGGCGCGGGCGTGGCATTCAATGGGCGGGCGCATATCGGCGGCAATGGCACGGCAGGTGAGTGGGGACACAATCAGCTACCGTGGATGGACGAAGACGAACTGCGTTATCGCGAGGAAGTCCCTTGTTATTGCGGTaa                                                                                   <  1:789441/148‑1 (MQ=255)
                                                              cACGGGTTGCGGCGCGGGCGTGGCATTCAATGGGCGGGCGCATATCGGCGGCAATGGCACGGCAGGTGAGTGGGGACACAATCAGCTACCGTGGATGGACGAAGACGAACTGCGTTATCGCGAGGAAGTCCCTTGTTATTGCGGTaaa                                                                                  <  2:2198221/148‑1 (MQ=255)
                                                              cACGGGATGCGGCGCGGGCGTGGCATTCAATGGGCGGGCGCATATCGGCGGCAATGGCACGGCAGGTGAGTGGGGACACAATCAGCTACCGTGGATGGACGAAGACGAACTGCGTTATCGCGAGGAAGTCCCTTGTTATTGCGGTaaa                                                                                  >  2:1239751/1‑148 (MQ=255)
                                                              cACGGGATGCGGCGCGGGCGTGGCATTCAATGGGCGGGCGCATATCGGCGGCAATGGCACGGCAGGTGAGTGGGGACACAATCAGCTACCGTGGATGGACGAAGACGAACTGCGTTATCGCGAGGAAGTCCCTTGTTATTGCGGTaaa                                                                                  <  1:5005/148‑1 (MQ=255)
                                                              cACGGGATGCGGCGCGGGCGTGGCATTCAATGGGCGGGCGCATATCGGCGGCAATGGCACGGCAGGTGAGTGGGGACACAATCAGCTACCGTGGATGGACGAAGACGAACTGCGTTATCGCGAGGAAGTCCCTTGTTATTGCGGTaaa                                                                                  <  2:2140484/148‑1 (MQ=255)
                                                                cGGGATGCGGCGCGGGCGTGGCATTCAATGGGCGGGCGCATATCGGCGGCAATGGCACGGCAGGTGAGTGGGGACACAATCAGCTACCGTGGATGGACGAAGACGAACTGCGTTATCGCGAGGAAGTCCCTTGTTATTGCGGTAAACa                                                                                >  1:3236142/1‑148 (MQ=255)
                                                                  ggATGCGGCGCGGGCGTGGCATTCAATGGGCGGGCGCATATCGGCGGCAATGGCACGGCAGGTGAGTGGGGACACACTCAGCTACCGTGGATGGACGAAGACGAACTGCGTTATCGCGAGGAAGTCCCTTGTTATTGCGGTAAACAAg                                                                              <  2:410089/148‑1 (MQ=255)
                                                                   gATGCGGCGCGGGCGTGGCATTCAATGGGCGGGCGCATATCGGCGGCAATGGCACGGCAGGTGAGTGGGGACACAATCAGCTACCGTGGATGGACGAAGACGAACTGCGTTATCGCGAGGAAGTCCCTTGTTATTGCGGTAAACAAgg                                                                             <  2:1070172/148‑1 (MQ=255)
                                                                   gATGCGGCGCGGGCGTGGCATTCAATGGGCGGGCGCATATCGGCGGCAATGGCACGGCAGGTGAGTGGGGACACAATCAGCTACCGTGGATGGACGAAGACGAACTGCGTTATCGCGAGGAAGTCCCTTGTTATTGCGGTAAACAAgg                                                                             <  1:1052478/148‑1 (MQ=255)
                                                                    aTGCGGCGCGGGCGTGGCATTCAATGGGCGGGCGCATATCGGCGGCAATGGCACGGCAGGTGAGTGGGGACACAATCAGCTACCGTGGATGGACGAAGACGAACTGCGTTATCGCGAGGAAGTCCCTTGTTATTGCGGTAAACAAGGt                                                                            >  2:2972931/1‑148 (MQ=255)
                                                                     tGCGGCGCGGGCGTGGCATTCAATGGGCGGGCGCATATCGGCGGCAATGGCACGGCAGGTGAGTGGGGACACAATCAGCTACCGTGGATGGACGAAGACGAACTGCGTTATCGCGAGGAAGTCCCTTGTTATTGCGGTAAACAAGGtt                                                                           <  2:2406101/148‑1 (MQ=255)
                                                                      gcggcgCGGGCGTGGCATTCAATGGGCGGGCGCATATCGGCGGCAATGGCACGGCAGGTGAGTGGGGACACAATCAGCTACCGTGGATGGACGAAGACGAACTGCGTTATCGCGAGGAAGTCCCTTGTTATTGCGGTAAACAAGGTTg                                                                          <  2:642077/148‑1 (MQ=255)
                                                                       cggcgCGGGCGTGGCATTCAATGGGCGGGCGCATATCGGCGGCAATGGCACGGCAGGTGAGTGGGGACACAATCAGCTACCGTGGATGGACGAAGACGAACTGCGTTATCGCGAGGAAGTCCCTTGTTATTGCGGTAAACAAGGTTGt                                                                         <  2:793985/148‑1 (MQ=255)
                                                                              ggCGTGGCATTCAATGGGCGGGCGCATATCGGCGGCAATGGCACGGCAGGTGAGTGGGGACACAATCAGCTACCGTGGATGGACGAAGACGAACTGCGTTATCGCGAGGAAGTCCCTTGTTATTGCGGTAAACAAGGTTGTATTGaaa                                                                  >  2:1054268/1‑148 (MQ=255)
                                                                              ggCGTGGCATTCAATGGGCGGGCGCATATCGGCGGCAATGGCACGGCAGGTGAGTGGGGACACAATCAGCTACCGTGGATGGACGAAGACGAACTGCGTTATCGCGAGGAAGTCCCTTGTTATTGCGGTAAACAAGGTTGTATTGaaa                                                                  >  1:1532534/1‑148 (MQ=255)
                                                                              ggCGTGGCATTCAATGGGCGGGCGCATATCGGCGGCAATGGCACGGCAGGTGAGTGGGGACACAATCAGCTACCGTGGATGGACGAAGACGAACTGCGTTATCGCGAGGAAGTCCCTTGTTATTGCGGTAAACAAGGTTGTATTGaaa                                                                  <  2:3225023/148‑1 (MQ=255)
                                                                               gCGTGGCATTCAATGGGCGGGCGCATATCGGCGGCAATGGCACGGCAGGTGAGTGGGGACACAATCAGCTACCGTGGATGGACGAAGACGAACTGCGTTATCGCGAGGAAGTCCCTTGTTATTGCGGTAAACAAGGTTGTATTGAAAc                                                                 <  2:551781/148‑1 (MQ=255)
                                                                               gCGTGGCATTCAATGGGCGGGCGCATATCGGCGGCAATGGCACGGCAGGTGAGTGGGGACACAATCAGCTACCGTGGATGGACGAAGACGAACTGCGTTATCGCGAGGAAGTCCCTTGTTATTGCGGTAAACAAGGTTGTATTGAAAc                                                                 <  2:551011/148‑1 (MQ=255)
                                                                               gCGTGGCATTCAATGGGCGGGCGCATATCGGCGGCAATGGCACGGCAGGTGAGTGGGGACACAATCAGCTACCGTGGATGGACGAAGACGAACTGCGTTATCGCGAGGAAGTCCCTTGTTATTGCGGTAAACAAGGTTGTATTGAAAc                                                                 <  1:1672313/148‑1 (MQ=255)
                                                                                cggggCATTCAATGGGCGGGCGCATATCGGCGGCAATGGCACGGCAGGTGAGTGGGGACACAATCAGCTACCGTGGATGGACGAAGACGAACTGCGTTATCGCGAGGAAGTCCCTTGTTATTGCGGTAAACAAGGTTGTATTGAAAcc                                                                >  1:2668158/4‑148 (MQ=255)
                                                                                cGTGGCATTCAATGGGCGGGCGCATATCGGCGGCAATGGCACGGCAGGTGAGTGGGGACACAATCAGCTACCGTGGATGGACGAAGACGAACTGCGTTATCGCGAGGAAGTCCCTTGTTATTGCGGTAAACAAGGTTGTATTGAAAcc                                                                <  2:3990/148‑1 (MQ=255)
                                                                                 gTGGCATTCAATGGGCGGGCGCATATCGGCGGCAATGGCACGGCAGGTGAGTGGGGACACAATCAGCTACCGTGGATGGACGAAGACGAACTGCGTTATCGCGAGGAAGTCCCTTGTTATTGCGGTAAACAAGGTTGTATTGAAACCt                                                               <  1:998301/148‑1 (MQ=255)
                                                                                 gTGGCATTCAATGGGCGGGCGCATATCGGCGGCAATGGCACGGCAGGTGAGTGGGGACACAATCAGCTACCGTGGATGGACGAAGACGAACTGCGTTATCGCGAGGAAGTCCCTTGTTATTGCGGTAAACAAGGTTGTATTGAAACCt                                                               <  1:1384550/148‑1 (MQ=255)
                                                                                  tGGCATTCAATGGGCGGGCGCATATCGGCGGCAATGGCACGGCAGGTGAGTGGGGACACAATCAGCTACCGTGGATGGACGAAGACGAACTGCGTTATCGCGAGGAAGTCCCTTGTTATTGCGGTAAACAAGGTTGTATTGAAACCtt                                                              >  2:1315051/1‑148 (MQ=255)
                                                                                  tGGCATTCAATGGGCGGGCGCATATCGGCGGCAATGGCACGGCAGGTGAGTGGGGACACAATCAGCTACCGTGGATGGACGAAGACGAACTGCGTTATCGCGAGGAAGTCCCTTGTTATTGCGGTAAACAAGGTTGTATTGAAACCtt                                                              <  1:1313298/148‑1 (MQ=255)
                                                                                   ggCATTCAATGGGCGGGCGCATATCGGCGGCAATGGCACGGCAGGTGAGTGGGGACACAATCAGCTACCGTGGATGGACGAAGACGAACTGCGTTATCGCGAGGAAGTCCCTTGTTATTGCGGTAAACAAGGTTGTATTGAAACCttt                                                             >  1:1330919/1‑148 (MQ=255)
                                                                                       ttCAATGGGCGGGCGCATATCGGCGGCAATGGCACGGCAGGTGAGTGGGGACACAATCAGCTACCGTGGATGGACGAAGACGAACTGCGTTATCGCGAGGAAGTCCCTTGTTATTGCGGTAAACAAGGTTGTATTGAAACCTTTAttt                                                         <  1:837724/148‑1 (MQ=255)
                                                                                         cAATGGGCGGGCGCATATCGGCGGCAATGGCACGGCAGGTGAGTGGGGACACAATCAGCTACCGTGGATGGACGAAGACGAACTGCGTTATCACGAGGACGTCCCTTGTTATTGCGGTAAACAAGGTTGTATTGAAACCGGTATTTCg                                                       >  2:2892756/1‑148 (MQ=255)
                                                                                              ggcgggcgCATATCGGCGGCAATGGCACGGCAGGTGAGTGGGGACACAATCAGCTACCGTGGATGGACGAAGACGAACTGCGTTATCGCGAGGAAGTCCCTTGTTATTGCGGTAAACAAGGTTGTATTGAAACCTTTATTTCGGGCAc                                                  >  1:1074653/1‑148 (MQ=255)
                                                                                                 gggcgCATATCGGCGGCAATGGCACGGCAGGTGAGTGGGGACACAATCAGCTACCGTGGATGGACGAAGACGAACTGCGTTATCGCGAGGAAGTCCCTTGTTATTGCGGTAAACAAGGTTGTATTGAAACCTTTATTTCGGGCACggg                                               <  1:1580883/148‑1 (MQ=255)
                                                                                                 gggcgCATATCGGCGGCAATGGCACGGCAGGTGAGTGGGGACACAATCAGCTACCGTGGATGGACGAAGACGAACTGCGTTATCGCGAGGAAGTCCCTTGTTATTGCGGTAAACAAGGTTGTATTGAAACCTTTATTTCGGGCACggg                                               <  1:389077/148‑1 (MQ=255)
                                                                                                   gcgcATATCGGCGGCAATGGCACGGCAGGTGAGTGGGGACACAATCAGCTACCGTGGATGGACGAAGACGAACTGCGTTATCGCGAGGAAGTCCCTTGTTATTGCGGTAAACAAGGTTGTATTGAAACCTTTATTTCGGGCACGGGAt                                             <  1:2767964/148‑1 (MQ=255)
                                                                                                   gcgcATATCGGCGGCAATGGCACGGCAGGTGAGTGGGGACACAATCAGCTACCGTGGATGGACGAAGACGAACTGCGTTATCGCGAGGAAGTCCCTTGTTATTGCGGTAAACAAGGTTGTATTGAAACCTTTATTTCGGGCACGGGAt                                             <  1:2768003/148‑1 (MQ=255)
                                                                                                     gcATATCGGCGGCTATGGCACGGCAGGTGAGTGGGGACACAATCAGCTACCGTGGATGGACGAAGACGAACTGCGTTATCGCGAGGAAGTCCCTTGTTATTGCGGTAAACAAGGTTGTATTGAAACCTTTATTTCGGGCACGGGATTc                                           <  2:372119/148‑1 (MQ=255)
                                                                                                     gcATATCGGCGGCAATGGCACGGCAGGTGAGTGGGGACACAATCAGCTACCGTGGATGGACGAAGACGAACTGCGTTATCGCGAGGAAGTCCCTTGTTATTGCGGTAAACAAGGTTGTATTGAAACCTTTATTTCGGGCACGGGATTc                                           <  2:2040090/148‑1 (MQ=255)
                                                                                                        tatCGGCGGCAATGGCACGGCAGGTGAGTGGGGACACAATCAGCTACCGTGGATGGACGAAGACGAACTGCGTTATCGCGAGGAAGTCCCTTGTTATTGCGGTAAACAAGGTTGTATTGAAACCTTTATTTCGGGCACGGGATTcgcg                                        <  1:1633846/148‑1 (MQ=255)
                                                                                                            ggcggcAATGGCACGGCAGGTGAGTGGGGACACAATCAGCTACCGTGGATGGACGAAGACGAACTGCGTTATCGCGAGGAAGTCCCTTGTTATTGCGGTAAACAAGGTTGTATTGAAACCTTTATTTCGGGCACGGGATTCGCGATgg                                    <  1:2400414/148‑1 (MQ=255)
                                                                                                              cggcAATGGCACGGCAGGTGAGTGGGGACACAATCAGCTACCGTGGATGGACGAAGACGAACTGCGTTATCGCGAGGAAGTCCCTTGTTATTGCGGTAAACAAGGTTGTATTGAAACCTTTATTTCGGGCACGGGATTCGCGATGGAt                                  <  2:2321470/148‑1 (MQ=255)
                                                                                                                  aaTGGCACGGCAGGTGAGTGGGGACACAATCAGCTACCGTGGATGGACGAAGACGAACTGCGTTATCGCGAGGAAGTCCCTTGTTATTGCGGTAAACAAGGTTGTATTGAAACCTTTATTTCGGGCACGGGATTCGCGATGGATTAtc                              <  2:1547369/148‑1 (MQ=255)
                                                                                                                   aTGGCACGGCAGGTGAGCGGGGACACAATCAGCTACCGTGGATGGACGAAGACGAACTGCGTTATCGCGAGGAAGTCCCTTGTTATTGCGGTAAACAAGGTTGTATTGAAACCTTTATTTCGGGCACGGGATTCGCGATGGATTAtcg                             >  1:537514/1‑148 (MQ=255)
                                                                                                                      gCACGGCAGGTGAGTGGGGACACAATCAGCTACCGTGGATGGACGAAGACGAACTGCGTTATCGCGAGGAAGTCCCTTGTTATTGCGGTAAACAAGGTTGTATTGAAACCTTTATTTCGGGCACGGGATTCGCGATGGATTAtcgtcg                          >  1:496446/1‑148 (MQ=255)
                                                                                                                       cACGGCAGGTGAGTGGGGACACAATCAGCTACCGTGGATGGACGAAGACGAACTGCGTTATCGCGAGGAAGTCCCTTGTTATTGCGGTAAACAAGGTTGTATTGAAACCTTTATTTCGGGCACGGGATTCGCGATGGATTATcgtcgt                         >  1:947511/1‑148 (MQ=255)
                                                                                                                          ggCAGGTGAGTGGGGACACAATCAGCTACCGTGGATGGACGAAGACGAACTGCGTTATCGCGAGGAAGTCCCTTGTTATTGCGGTAAACAAGGTTGTATTGAAACCTTTATTTCGGGCACGGGATTCGCGATGGATTATCGTCGTTTg                      <  2:3245168/148‑1 (MQ=255)
                                                                                                                            cAGGTGAGTGGGGACACAATCAGCTACCGTGGATGGACGAAGACGAACTGCGTTATCGCGAGGAAGTCCCTTGTTATTGCGGTAAACAAGGTTGTATTGAAACCTTTATTTCGGGCACGGGATTCGCGATGGATTATCGTCGTTTGAg                    <  2:653197/148‑1 (MQ=255)
                                                                                                                            cAGGTGAGTGGGGACACAATCAGCGACCGTGGATGGACGAAGACGAACTGCGTTATCGCGAGGAAGTCCCTTGTTATTGCGGTAAACAAGGTTGTATTGAAACCTTTATTTCGGGCACGGGATTCGCGATGGATTATCGTCGTTTGAg                    <  2:2342064/148‑1 (MQ=255)
                                                                                                                             aGGTGAGTGGGGACACAATCAGCTACCGTGGATGGACGAAGACGAACTGCGTTATCGCGAGGAAGTCCCTTGTTATTGCGGTAAACAAGGTTGTATTGAAACCTTTATTTCGGGCACGGGATTCGCGATGGATTATCGTCGTTTGAGc                   <  2:2473595/148‑1 (MQ=255)
                                                                                                                              ggTGAGTGGGGACACAATCAGCTACCGTGGATGGACGAAGACGAACTGCGTTATCGCGAGGAAGTCCCTTGTTATTGCGGTAAACAAGGTTGTATTGAAACCTTTATTTCGGGCACGGGATTCGCGATGGATTATCGTCGTTTGTGCg                  >  1:365297/1‑148 (MQ=255)
                                                                                                                              ggTGAGTGGGGACACAATCAGCTACCGTGGATGGACGAAGACGAACTGCGTTATCGCGAGGAAGTCCCTTGTTATTGCGGTAAACAAGGTTGTATTGAAACCTTTATTTCGGGCACGGGATTCGCGATGGATTATCGTCGTTTGAGCg                  >  1:365311/1‑148 (MQ=255)
                                                                                                                              ggTGAGTGGGGACACAATCAGCTACCGTGGATGGACGAAGACGAACTGCGTTATCGCGAGGAAGTCCCTTGTTATTGCGGTAAACAAGGTTGTATTGAAACCTTTATTTCGGGCACGGGATTCGCGATGGATTATCGTCGTTTGAGCg                  >  1:2460853/1‑148 (MQ=255)
                                                                                                                               gTGAGTGGGGACACAATCAGCTACCGTGGATGGACGAAGACGAACTGCGTTATCGCGAGGAAGTCCCTTGTTATTGCGGTAAACAAGGTTGTATTGAAACCTTTATTTCGGGCACGGGATTCGCGATGGATTATCGTCGTTTGAGCgg                 <  2:445982/148‑1 (MQ=255)
                                                                                                                                  aGTGGGGACACAATCAGCTACCGTGGATGGACGAAGACGAACTGCGTTATCGCGAGGAAGTCCCTTGTTATTGCGGTAAACAAGGTTGTATTGAAACCTTTATTTCGGGCACGGGATTCGCGATGGATTATCGTCGTTTGAGCGGACa              <  1:769118/148‑1 (MQ=255)
                                                                                                                                   gTGGGGACACAATCAGCTACCGTGGATGGACGAAGACGAACTGCGTTATCGCGAGGAAGTCCCTTGTTATTGCGGTAAACAAGGTTGTATTGAAACCTTTATTTCGGGCACGGGATTCGCGATGGATTATCGTCGTTTGAGCGGACAt             <  1:1443556/148‑1 (MQ=255)
                                                                                                                                       ggACACAATCAGCTACCGTGGATGGACGAAGACGAACTGCGTTATCGCGAGGAAGTCCCTTGTTATTGCGGTAAACAAGGTTGTATTGAAACCTTTATTTCGGGCACGGGATTCGCGATGGATTATCGTCGTTTGAGCGGACATgcgc         <  2:1454730/148‑1 (MQ=255)
                                                                                                                                         acacAATCAGCTACCGTGGATGGACGAAGACGAACTGCGTTATCGCGAGGAAGTCCCTTGTTATTGCGGTAAACAAGGTTGTATTGAAACCTTTATTTCGGGCACGGGATTCGCGATGGATTATCGTCGTTTGAGCGGACATGCGCTg       <  1:1157382/148‑1 (MQ=255)
                                                                                                                                            caATCAGCTACCGTGGATGGACGAAGACGAACTGCGTTATCGCGAGGAAGTCCCTTGTTATTGCGGTAAACAAGGTTGTATTGAAACCTTTATTTCGGGCACGGGATTCGCGATGGATTATCGTCGTTTGAGCGGACATGCGCTGaaa    >  2:280821/1‑148 (MQ=255)
                                                                                                                                             aaTCAGCTACCGTGGATGGACGAAGACGAACTGCGTTATCGCGAGGAAGTCCCTTGTTATTGCGGTAAACAAGGTTGTATTGAAACCTTTATTTCGGGCACGGGATTCGCGATGGATTATCGTCGTTTGAGCGGACATGCGCTGAAAg   <  2:2764865/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
GTTTCAGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATCGGCACGGGATGCGGCGCGGGCGTGGCATTCAATGGGCGGGCGCATATCGGCGGCAATGGCACGGCAGGTGAGTGGGGACACAATCCGCTACCGTGGATGGACGAAGACGAACTGCGTTATCGCGAGGAAGTCCCTTGTTATTGCGGTAAACAAGGTTGTATTGAAACCTTTATTTCGGGCACGGGATTCGCGATGGATTATCGTCGTTTGAGCGGACATGCGCTGAAAGG  >  CP009273/405930‑406219

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GGCGGTTTCAGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATCGGCACGGGATGCGGCGCGGGCGTGGCATTCAATGGGCGGGCGCATATCGGCGGCAATGGCACGGCAGGTGAGTGGGGACACAATCCGCTACCGTGGATGGACGAAGACGAACTGCGTTATCGCGAGGAAGTCCCTTGTTATTGCGGTAAACAAGGTTGTATTGAAACCTTTATTTCGGGCACGGGATTCGCGATGGATTATCGTCGTTTGAGCGGACATGCGCTGAAAGGCAG  >  CP009273/405926‑406222
                                                                                                                                                     |                                                                                                                                                   
GGCGGTTTCAGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATCGGCACGGGATGCGGCGCGGGCGTGGCATTCAATGGGCGGGCGCATATCGGCGGCAATGGCACGGCAGGTGAGTGGGGACACAATCA                                                                                                                                                     <  SRR19973070.1769148/150‑1 (MQ=60)
 GCGGTTTCAGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATCGGCACGGGATGCGGCGCGGGCGTGGCATTCAATGGGCGGGCGCATATCGGCGGCAATGGCACGGCAGGTGAGTGGGGACACAATCAG                                                                                                                                                    <  SRR19973070.2980008/150‑1 (MQ=60)
  CGGTTTCAGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATCGGCACGGGATGCGGCGCGGGCGTGGCATTCAATGGGCGGGCGCATATCGGCGGCAATGGCACGGCAGGTGAGTGGGGACACAATCAGC                                                                                                                                                   <  SRR19973070.2870538/150‑1 (MQ=60)
  CGGTTTCAGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATCGGCACGGGATGCGGCGCGGGCGTGGCATTCAATGGGCGGGCGCATATCGGCGGCAATGGCACGGCAGGTGAGTGGGGACACAATCAGC                                                                                                                                                   >  SRR19973070.623775/1‑150 (MQ=60)
   GGTTTCAGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATCGGCACGGGATGCGGCGCGGGCGTGGCATTCAATGGGCGGGCGCATATCGGCGGCAATGGCACGGCAGGTGAGTGGGGACACAATCAGCT                                                                                                                                                  >  SRR19973070.977787/1‑150 (MQ=60)
   GGTTTCAGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATCGGCACGGGATGCGGCGCGGGCGTGGCATTCAATGGGCGGGCGCATATCGGCGGCAATGGCACGGCAGGTGAGTGGGGACACAATCAGCT                                                                                                                                                  >  SRR19973070.810301/1‑150 (MQ=60)
   GGTTTCAGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATCGGCACGGGATGCGGCGCGGGCGTGGCATTCAATGGGCGGGCGCATATCGGCGGCAATGGCACGGCAGGTGAGTGGGGACACAATCAGCT                                                                                                                                                  >  SRR19973070.1558252/1‑150 (MQ=60)
   GGTTTCAGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATCGGCACGGGATGCGGCGCGGGCGTGGCATTCAATGGGCGGGCGCATATCGGCGGCAATGGCACGGCAGGTGAGTGGGGACACAATCAGCT                                                                                                                                                  >  SRR19973070.3252645/1‑150 (MQ=60)
        CAGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATCGGCACGGGATGCGGCGCGGGCGTGGCATTCAATGGGCGGGCGCATATCGGCGGCAATGGCACGGCAGGTGAGTGGGGACACAATCAGCTACCGT                                                                                                                                             >  SRR19973070.1303157/1‑150 (MQ=60)
           AAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATCGGCACGGGATGCGGCGCGGGCGTGGCATTCAATGGGCGGGCGCATATCGGCGGCAATGGCACGGCAGGTGAGTGGGGACACAATCAGCTACCGTGGA                                                                                                                                          >  SRR19973070.3225761/1‑150 (MQ=60)
           AAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATCGGCACGGGATGCGGCGCGGGCGTGGCATTCAATGGGCGGGCGCATATCGGCGGCAATGGCACGGCAGGTGAGTGGGGACACAATCAGCTACCGTGGA                                                                                                                                          >  SRR19973070.3225763/1‑150 (MQ=60)
            AGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATCGGCACGGGATGCGGCGCGGGCGTGGCATTCAATGGGCGGGCGCATATCGGCGGCAATGGCACGGCAGGTGAGTGGGGACACAATCAGCTACCGTGGAT                                                                                                                                         >  SRR19973070.2489711/1‑150 (MQ=60)
               AGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATCGGCACGGGATGCGGCGCGGGCGTGGCATTCAATGGGCGGGCGCATATCGGCGGCAATGGCACGGCAGGTGAGTGGGGACACAATCAGCTACCGTGGATGGA                                                                                                                                      >  SRR19973070.2864378/1‑150 (MQ=60)
               AGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATCGGCACGGGATGCGGCGCGGGCGTGGCATTCAATGGGCGGGCGCATATCGGCGGCAATGGCACGGCAGGTGAGTGGGGACACAATCAGCTACCGTGGATGGA                                                                                                                                      >  SRR19973070.127441/1‑150 (MQ=60)
                 TAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATCGGCACGGGATGCGGCGCGGGCGTGGCATTCAATGGGCGGGCGCATATCGGCGGCAATGGCACGGCAGGTGAGTGGGGACACAATCAGCTACCGTGGATGGACG                                                                                                                                    <  SRR19973070.1460906/150‑1 (MQ=60)
                 TAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATCGGCACGGGATGCGGCGCGGGCGTGGCATTCAATGGGCGGGCGCATATCGGCGGCAATGGCACGGCAGGTGAGTGGGGACACAATCAGCTACCGTGGATGGACG                                                                                                                                    <  SRR19973070.1461076/150‑1 (MQ=60)
                 TAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATCGGCACGGGATGCGGCGCGGGCGTGGCATTCAATGGGCGGGCGCATATCGGCGGCAATGGCACGGCAGGTGAGTGGGGACACAATCAGCTACCGTGGATGGACG                                                                                                                                    <  SRR19973070.2322712/150‑1 (MQ=60)
                 TAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATCGGCACGGGATGCGGCGCGGGCGTGGCATTCAATGGGCGGGCGCATATCGGCGGCAATGGCACGGCAGGTGAGTGGGGACACAATCAGCTACCGTGGATGGACG                                                                                                                                    <  SRR19973070.3116468/150‑1 (MQ=60)
                  AGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATCGGCACGGGATGCGGCGCGGGCGTGGCATTCAATGGGCGGGCGCATATCGGCGGCAATGGCACGGCAGGTGAGTGGGGACACAATCAGCTACCGTGGATGGACGA                                                                                                                                   >  SRR19973070.2186074/1‑150 (MQ=60)
                  AGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATCGGCACGGGATGCGGCGCGGGCGTGGCATTCAATGGGCGGGCGCATATCGGCGGCAATGGCACGGCAGGTGAGTGGGGACACAATCAGCTACCGTGGATGGACGA                                                                                                                                   >  SRR19973070.2186048/1‑150 (MQ=60)
                    ATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATCGGCACGGGATGCGGCGCGGGCGTGGCATTCAATGGGCGGGCGCATATCGGCGGCAATGGCACGGCAGGTGAGTGGGGACACAATCAGCTACCGTGGATGGACGAAG                                                                                                                                 <  SRR19973070.784600/150‑1 (MQ=60)
                     TGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATCGGCACGGGATGCGGCGCGGGCGTGGCATTCAATGGGCGGGCGCATATCGGCGGCAATGGCACGGCAGGTGAGTGGGGACACAATCAGCTACCGTGGATGGACGAAGA                                                                                                                                <  SRR19973070.481947/150‑1 (MQ=60)
                     TGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATCGGCACGGGATGCGGCGCGGGCGTGGCATTCAATGGGCGGGCGCATATCGGCGGCAATGGCACGGCAGGTGAGTGGGGACACAATCAGCTACCGTGGATGGACGAAGA                                                                                                                                <  SRR19973070.482027/150‑1 (MQ=60)
                       GCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATCGGCACGGGATGCGGCGCGGGCGTGGCATTCAATGGGCGGGCGCATATCGGCGGCAATGGCACGGCAGGTGAGTGGGGACACAATCAGCTACCGTGGATGGACGAAGACG                                                                                                                              <  SRR19973070.960678/150‑1 (MQ=60)
                         GCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATCGGCACGGGATGCGGCGCGGGCGTGGCATTCAATGGGCGGGCGCATATCGGCGGCAATGGCACGGCAGGTGAGTGGGGACACAATCAGCTACCGTGGATGGACGAAGACGAA                                                                                                                            <  SRR19973070.248945/150‑1 (MQ=60)
                             CAGCGGGAGCGCAGACGGTATTTGCCGTGATTATCGGCACGGGATGCGGCGCGGGCGTGGCATTCAATGGGCGGGCGCATATCGGCGGCAATGGCACGGCAGGTGAGTGGGGACACAATCAGCTACCGTGGATGGACGAAGACGAACTGC                                                                                                                        >  SRR19973070.2718262/1‑150 (MQ=60)
                                CGGGAGCGCAGACGGTATTTGCCGTGATTATCGGCACGGGATGCGGCGCGGGCGTGGCATTCAATGGGCGGGCGCATATCGGCGGCAATGGCACGGCAGGTGAGTGGGGACACAATCAGCTACCGTGGATGGACGAAGACGAACTGCGTT                                                                                                                     >  SRR19973070.62387/1‑150 (MQ=60)
                                CGGGAGCGCAGACGGTATTTGCCGTGATTATCGGCACGGGATGCGGCGCGGGCGTGGCATTCAATGGGCGGGCGCATATCGGCGGCAATGGCACGGCAGGTGAGTGGGGACACAATCAGCTACCGTGGATGGACGAAGACGAACTGCGTT                                                                                                                     <  SRR19973070.516084/150‑1 (MQ=60)
                                   GAGCGCAGACGGTATTTGCCGTGATTATCGGCACGGGATGCGGCGCGGGCGTGGCATTCAATGGGCGGGCGCATATCGGCGGCAATGGCACGGCAGGTGAGTGGGGACACAATCAGCTACCGTGGATGGACGAAGACGAACTGCGTTATC                                                                                                                  <  SRR19973070.2303920/150‑1 (MQ=60)
                                   GAGCGCAGACGGTATTTGCCGTGATTATCGGCACGGGATGCGGCGCGGGCGTGGCATTCAATGGGCGGGCGCATATCGGCGGCAATGGCACGGCAGGTGAGTGGGGACACAATCAGCTACCGTGGATGGACGAAGACGAACTGCGTTATC                                                                                                                  <  SRR19973070.2303864/150‑1 (MQ=60)
                                    AGCGCAGACGGTATTTGCCGTGATTATCGGCACGGGATGCGGCGCGGGCGTGGCATTCAATGGGCGGGCGCATATCGGCGGCAATGGCACGGCAGGTGAGTGGGGACACAATCAGCTACCGTGGATGGACGAAGACGAACTGCGTTATCG                                                                                                                 >  SRR19973070.1622701/1‑150 (MQ=60)
                                        CAGACGGTATTTGCCGTGATTATCGGCACGGGATGCGGCGCGGGCGTGGCATTCAATGGGCGGGCGCATATCGGCGGCAATGGCACGGCAGGTGAGTGGGGACACAATCAGCTACCGTGGATGGACGAAGACGAACTGCGTTATCGCGAG                                                                                                             >  SRR19973070.233375/1‑150 (MQ=60)
                                        CAGACGGTATTTGCCGTGATTATCGGCACGGGATGCGGCGCGGGCGTGGCATTCAATGGGCGGGCGCATATCGGCGGCAATGGCACGGCAGGTGAGTGGGGACACAATCAGCTACCGTGGATGGACGAAGACGAACTGCGTTATCGCGAG                                                                                                             >  SRR19973070.233188/1‑150 (MQ=60)
                                        CAGACGGTATTTGCCGTGATTATCGGCACGGGATGCGGCGCGGGCGTGGCATTCAATGGGCGGGCGCATATCGGCGGCAATGGCACGGCAGGTGAGTGGGGACACAATCAGCTACCGTGGATGGACGAAGACGAACTGCGTTATCGCGAG                                                                                                             <  SRR19973070.559705/150‑1 (MQ=60)
                                           ACGGTATTTGCCGTGATTATCGGCACGGGATGCGGCGCGGGCGTGGCATTCAATGGGCGGGCGCATATCGGCGGCAATGGCACGGCAGGTGAGTGGGGACACAATCAGCTACCGTGGATGGACGAAGACGAACTGCGTTATCGCGAGGAA                                                                                                          <  SRR19973070.1702275/150‑1 (MQ=60)
                                            CGGTATTTGCCGTGATTATCGGCACGGGATGCGGCGCGGGCGTGGCATTCAATGGGCGGGCGCATATCGGCGGCAATGGCACGGCAGGTGAGTGGGGACACAATCAGCTACCGTGGATGGACGAAGACGAACTGCGTTATCGCGAGGAAG                                                                                                         <  SRR19973070.2679564/150‑1 (MQ=60)
                                               TATTTGCCGTGATTATCGGCACGGGATGCGGCGCGGGCGTGGCATTCAATGGGCGGGCGCATATCGGCGGCAATGGCACGGCAGGTGAGTGGGGACACAATCAGCTACCGTGGATGGACGAAGACGAACTGCGTTATCGCGAGGAAGTCC                                                                                                      <  SRR19973070.2485684/150‑1 (MQ=60)
                                                ATTTGCCGTGATTATCGGCACGGGATGCGGCGCGGGCGTGGCATTCAATGGGCGGGCGCATATCGGCGGCAATGGCACGGCAGGTGAGTGGGGACACAATCAGCTACCGTGGATGGACGAAGACGAACTGCGTTATCGCGAGGAAGTCCC                                                                                                     <  SRR19973070.2206299/150‑1 (MQ=60)
                                                ATTTGCCGTGATTATCGGCACGGGATGCGGCGCGGGCGTGGCATTCAATGGGCGGGCGCATATCGGCGGCAATGGCACGGCAGGTGAGTGGGGACACAATCAGCTACCGTGGATGGACGAAGACGAACTGCGTTATCGCGAGGAAGTCCC                                                                                                     <  SRR19973070.3063779/150‑1 (MQ=60)
                                                   TGCCGTGATTATCGGCACGGGATGCGGCGCGGGCGTGGCATTCAATGGGCGGGCGCATATCGGCGGCAATGGCACGGCAGGTGAGTGGGGACACAATCAGCTACCGTGGATGGACGAAGACGAACTGCGTTATCGCGAGGAAGTCCCTTG                                                                                                  <  SRR19973070.330408/150‑1 (MQ=60)
                                                    GCCGTGATTATCGGCACGGGATGCGGCGCGGGCGTGGCATTCAATGGGCGGGCGCATATCGGCGGCAATGGCACGGCAGGTGAGTGGGGACACAATCAGCTACCGTGGATGGACGAAGACGAACTGCGTTATCGCGAGGAAGTCCCTTGT                                                                                                 <  SRR19973070.1606933/150‑1 (MQ=60)
                                                     CCGTGATTATCGGCACGGGATGCGGCGCGGGCGTGGCATTCAATGGGCGGGCGCATATCGGCGGCAATGGCACGGCAGGTGAGTGGGGACACAATCAGCTACCGTGGATGGACGAAGACGAACTGCGTTATCGCGAGGAAGTCCCTTGTT                                                                                                <  SRR19973070.1345948/150‑1 (MQ=60)
                                                      CGTGATTATCGGCACGGGATGCGGCGCGGGCGTGGCATTCAATGGGCGGGCGCATATCGGCGGCAATGGCACGGCAGGTGAGTGGGGACACAATCAGCTACCGTGGATGGACGAAGACGAACTGCGTTATCGCGAGGAAGTCCCTTGTTA                                                                                               <  SRR19973070.1578810/150‑1 (MQ=60)
                                                      CGTGATTATCGGCACGGGATGCGGCGCGGGCGTGGCATTCAATGGGCGGGCGCATATCGGCGGCAATGGCACGGCAGGTGAGTGGGGACACAATCAGCTACCGTGGATGGACGAAGACGAACTGCGTTATCGCGAGGAAGTCCCTTGTTA                                                                                               >  SRR19973070.2836521/1‑150 (MQ=60)
                                                      AGTGATTATCGGGACTGGATGCGGCGCGTGCGTGGCATTCTATGGGCGGGCGCTTATCGGCGGCTATTGCACGGCAGGTGAGTGGGGACACAATCAGCTTCCGTGGATGGACTAAGACGAAATGCGTTATCGCGAGGTAGTCACTTGTTA                                                                                               <  SRR19973070.1578845/150‑1 (MQ=60)
                                                       GTGATTATCGGCACGGGATGCGGCGCGGGCGTGGCATTCAATGGGCGGGCGCATATCGGCGGCAATGGCACGGCAGGTGAGTGGGGACACAATCAGCTACCGTGGATGGACGAAGACGAACTGCGTTATCGCGAGGAAGTCCCTTGTTAT                                                                                              >  SRR19973070.3266083/1‑150 (MQ=60)
                                                       GTGATTATCGGCACGGGATGCGGCGCGGGCGTGGCATTCAATGGGCGGGCGCATATCGGCGGCAATGGCACGGCAGGTGAGTGGGGACACAATCAGCTACCGTGGATGGACGAAGACGAACTGCGTTATCGCGAGGAAGTCCCTTGTTAT                                                                                              >  SRR19973070.2074599/1‑150 (MQ=60)
                                                       GTGATTATCGGCACGGGATGCGGCGCGGGCGTGGCATTCAATGGGCGGGCGCATATCGGCGGCAATGGCACGGCAGGTGAGTGGGGACACAATCAGCTACCGTGGATGGACGAAGACGAACTGCGTTATCGCGAGGAAGTCCCTTGTTAT                                                                                              >  SRR19973070.2120601/1‑150 (MQ=60)
                                                       GTGATTATCGGCACGGGATGCGGCGCGGGCGTGGCATTCAATGGGCGGGCGCATATCGGCGGCAATGGCACGGCAGGTGAGTGGGGACACAATCAGCTACCGTGGATGGACGAAGACGAACTGCGTTATCGCGAGGAAGTCCCTTGTTAT                                                                                              >  SRR19973070.2120870/1‑150 (MQ=60)
                                                        TGATTATCGGCACGGGTTGCGGCGCGGGCGTGGCATTAAATGGTCGTGCGCATATCGGCGGCATTGGAACTGCAGGTGAGTGGGGACTCAATCAGCTACATTGGATGGACGAAGACGAACTGCGTTATCGAGAGGAAGTCCCTTGTTATT                                                                                             <  SRR19973070.824625/150‑1 (MQ=60)
                                                        TGATTATCGGCACGGGATGCGGCGCGGGCGTGGCATTCAATGGGCGGGCGCATATCGGCGGCAATGGCACGGCAGGTGAGTGGGGACACAATCAGCTACCGTGGATGGACGAAGACGAACTGCGTTATCGCGAGGAAGTCCCTTGTTATT                                                                                             <  SRR19973070.1307505/150‑1 (MQ=60)
                                                         GATTATCGGCACGGGATGCGGCGCGGGCGTGGCATTCAATGGGCGGGCGCATATCGGCGGCAATGGCACGGCAGGTGAGTGGGGACACAATCAGCTACCGTGGATGGACGAAGACGAACTGCGTTATCGCGAGGAAGTCCCTTGTTTTTG                                                                                            <  SRR19973070.412482/150‑1 (MQ=60)
                                                               CGGCACGGGATGCGGCGCGGGCGTGGCATTCAATGGGCGGGCGCATATCGGCGGCAATGGCACGGCAGGTGAGTGGGGACACAATCAGCTACCGTGGATGGACGAAGACGAACTGCGTTATCGCGAGGAAGTCCCTTGTTATTGCGGTAA                                                                                      <  SRR19973070.186471/150‑1 (MQ=60)
                                                                GGCACGGGATGCGGCGCGGGCGTGGCATTCAATGGGCGGGCGCATATCGGCGGCAATGGCACGGCAGGTGAGTGGGGACACAATCAGCTACCGTGGATGGACGAAGACGAACTGCGTTATCGCGAGGAAGTCCCTTGTTATTGCGGTAAA                                                                                     <  SRR19973070.1410418/150‑1 (MQ=60)
                                                                 GCACGGGATGCGGCGCGGGCGTGGCATTCAATGGGCGGGCGCATATCGGCGGCAATGGCACGGCAGGTGAGTGGGGACACAATCAGCTACCGTGGATGGACGAAGACGAACTGCGTTATCGCGAGGAAGTCCCTTGTTATTGCGGTAAAC                                                                                    <  SRR19973070.794916/150‑1 (MQ=60)
                                                                  CACGGGATGCGGCGCGGGCGTGGCATTCAATGGGCGGGCGCATATCGGCGGCAATGGCACGGCAGGTGAGTGGGGACACAATCAGCTACCGTGGATGGACGAAGACGAACTGCGTTATCGCGAGGAAGTCCCTTGTTATTGCGGTAAACA                                                                                   <  SRR19973070.5086/150‑1 (MQ=60)
                                                                  CACGGGATGCGGCGCGGGCGTGGCATTCAATGGGCGGGCGCATATCGGCGGCAATGGCACGGCAGGTGAGTGGGGACACAATCAGCTACCGTGGATGGACGAAGACGAACTGCGTTATCGCGAGGAAGTCCCTTGTTATTGCGGTAAACA                                                                                   >  SRR19973070.3257027/1‑150 (MQ=60)
                                                                    CGGGATGCGGCGCGGGCGTGGCATTCAATGGGCGGGCGCATATCGGCGGCAATGGCACGGCAGGTGAGTGGGGACACAATCAGCTACCGTGGATGGACGAAGACGAACTGCGTTATCGCGAGGAAGTCCCTTGTTATTGCGGTAAACAAG                                                                                 >  SRR19973070.380356/1‑150 (MQ=60)
                                                                       GATGCGGCGCGGGCGTGGCATTCAATGGGCGGGCGCATATCGGCGGCAATGGCACGGCAGGTGAGTGGGGACACAATCAGCTACCGTGGATGGACGAAGACGAACTGCGTTATCGCGAGGAAGTCCCTTGTTATTGCGGTAAACAAGGTT                                                                              <  SRR19973070.1059878/150‑1 (MQ=60)
                                                                                CGGGCGTGGCATTCAATGGGCGGGCGCATATCGGCGGCAATGGCACGGCAGGTGAGTGGGGACACAATCAGCTACCGTGGATGGACGAAGACGAACTGCGTTATCGCGAGGAAGTCCCTTGTTATTGCGGTAAACAAGGTTGTATTGAAA                                                                     >  SRR19973070.1543296/1‑150 (MQ=60)
                                                                                  GGCGGGGCATTCAATGGGCGGGCGCATATCGGCGGCAATGGCACGGCAGGTGAGTGGGGACACAATCAGCTACCGTGGATGGACGAAGACGAACTGCGTTATCGCGAGGAAGTCCCTTGTTATTGCGGTAAACAAGGTTGTATTGAAACC                                                                   >  SRR19973070.2685486/1‑150 (MQ=60)
                                                                                   GCGTGGCATTCAATGGGCGGGCGCATATCGGCGGCAATGGCACGGCAGGTGAGTGGGGACACAATCAGCTACCGTGGATGGACGAAGACGAACTGCGTTATCGCGAGGAAGTCCCTTGTTATTGCGGTAAACAAGGTTGTATTGAAACCT                                                                  <  SRR19973070.1683777/150‑1 (MQ=60)
                                                                                     GTGGCATTCAATGGGCGGGCGCATATCGGCGGCAATGGCACGGCAGGTGAGTGGGGACACAATCAGCTACCGTGGATGGACGAAGACGAACTGCGTTATCGCGAGGAAGTCCCTTGTTATTGCGGTAAACAAGGTTGTATTGAAACCTTT                                                                >  SRR19973070.1340096/1‑150 (MQ=60)
                                                                                     GTGGCATTCAATGGGCGGGCGCATATCGGCGGCAATGGCACGGCAGGTGAGTGGGGACACAATCAGCTACCGTGGATGGACGAAGACGAACTGCGTTATCGCGAGGAAGTCCCTTGTTATTGCGGTAAACAAGGTTGTATTGAAACCTTT                                                                <  SRR19973070.1393919/150‑1 (MQ=60)
                                                                                     GTGGCATTCAATGGGCGGGCGCATATCGGCGGCAATGGCACGGCAGGTGAGTGGGGACACAATCAGCTACCGTGGATGGACGAAGACGAACTGCGTTATCGCGAGGAAGTCCCTTGTTATTGCGGTAAACAAGGTTGTATTGAAACCTTT                                                                <  SRR19973070.1005421/150‑1 (MQ=60)
                                                                                      TGGCATTCAATGGGCGGGCGCATATCGGCGGCAATGGCACGGCAGGTGAGTGGGGACACAATCAGCTACCGTGGATGGACGAAGACGAACTGCGTTATCGCGAGGAAGTCCCTTGTTATTGCGGTAAACAAGGTTGTATTGAAACCTTTA                                                               <  SRR19973070.1322404/150‑1 (MQ=60)
                                                                                       GGCATTCAATGGGCGGGCGCATATCGGCGGCAATGGCACGGCAGGTGAGTGGGGACACAATCAGCTACCGTGGATGGACGAAGACGAACTGCGTTATCGCGAGGAAGTCCCTTGTTATTGCGGTAAACAAGGTTGTATTGAAACCTTTAT                                                              <  SRR19973070.3159676/150‑1 (MQ=60)
                                                                                           TTCAATGGGCGGGCGCATATCGGCGGCAATGGCACGGCAGGTGAGTGGGGACACAATCAGCTACCGTGGATGGACGAAGACGAACTGCGTTATCGCGAGGAAGTCCCTTGTTATTGCGGTAAACAAGGTTGTATTGAAACCTTTATTTCG                                                          <  SRR19973070.843387/150‑1 (MQ=60)
                                                                                           TTCAATGGGCGGGCGCATATCGGCGGCAATGGCACGGCAGGTGAGTGGGGACACAATCAGCTACCGTGGATGGACGAAGACGAACTGCGTTATCGCGAGGAAGTCCCTTGTTATTGCGGTAAACAAGGTTGTATTGAAACCTTTATTTCG                                                          <  SRR19973070.843138/150‑1 (MQ=60)
                                                                                                TGGGCGGGCGCATATCGGCGGCAATGGCACGGCAGGTGAGTGGGGACACAATCAGCTACCGTGGATGGACGAAGACGAACTGCGTTATCGCGAGGAAGTCCCTTGTTATTGCGGTAAACAAGGTTGTATTGAAACCTTTATTTCGGGCAC                                                     >  SRR19973070.1082142/1‑150 (MQ=60)
                                                                                                     GGGCGCATATCGGCGGCAATGGCACGGCAGGTGAGTGGGGACACAATCAGCTACCGTGGATGGACGAAGACGAACTGCGTTATCGCGAGGAAGTCCCTTGTTATTGCGGTAAACAAGGTTGTATTGAAACCTTTATTTCGGGCACGGGAT                                                <  SRR19973070.1591967/150‑1 (MQ=60)
                                                                                                     GGGCGCATATCGGCGGCAATGGCACGGCAGGTGAGTGGGGACACAATCAGCTACCGTGGATGGACGAAGACGAACTGCGTTATCGCGAGGAAGTCCCTTGTTATTGCGGTAAACAAGGTTGTATTGAAACCTTTATTTCGGGCACGGGAT                                                <  SRR19973070.2292694/150‑1 (MQ=60)
                                                                                                     GGGCGCATATCGGCGGCAATGGCACGGCAGGTGAGTGGGGACACAATCAGCTACCGTGGATGGACGAAGACGAACTGCGTTATCGCGAGGAAGTCCCTTGTTATTGCGGTAAACAAGGTTGTATTGAAACCTTTATTTCGGGCACGGGAT                                                <  SRR19973070.391926/150‑1 (MQ=60)
                                                                                                       GCGCATATCGGCGGCAATGGCACGGCAGGTGAGTGGGGACACAATCAGCTACCGTGGATGGACGAAGACGAACTGCGTTATCGCGAGGAAGTCCCTTGTTATTGCGGTAAACAAGGTTGTATTGAAACCTTTATTTCGGGCACGGGATTC                                              <  SRR19973070.2785685/150‑1 (MQ=60)
                                                                                                       GCGCATATCGGCGGCAATGGCACGGCAGGTGAGTGGGGACACAATCAGCTACCGTGGATGGACGAAGACGAACTGCGTTATCGCGAGGAAGTCCCTTGTTATTGCGGTAAACAAGGTTGTATTGAAACCTTTATTTCGGGCACGGGATTC                                              <  SRR19973070.2785724/150‑1 (MQ=60)
                                                                                                          CATATCGGCGGCAATGGCACGGCAGGTGAGTGGGGACACAATCAGCTACCGTGGATGGACGAAGACGAACTGCGTTATCGCGAGGAAGTCCCTTGTTATTGCGGTAAACAAGGTTGTATTGAAACCTTTATTTCGGGCACGGGATTCGCG                                           >  SRR19973070.2192220/1‑150 (MQ=60)
                                                                                                            TATCGGCGGCAATGGCACGGCAGGTGAGTGGGGACACAATCAGCTACCGTGGATGGACGAAGACGAACTGCGTTATCGCGAGGAAGTCCCTTGTTATTGCGGTAAACAAGGTTGTATTGAAACCTTTATTTCGGGCACGGGATTCGCGAT                                         <  SRR19973070.1645176/150‑1 (MQ=60)
                                                                                                               CGGCGGCAATGGCACGGCAGGTGAGTGGGGACACAATCAGCTACCGTGGATGGACGAAGACGAACTGCGTTATCGCGAGGAAGTCCCTTGTTATTGCGGTAAACAAGGTTGTATTGAAACCTTTATTTCGGGCACGGGATTCGCGATGGA                                      >  SRR19973070.3306622/1‑150 (MQ=60)
                                                                                                                GGCGGCAATGGCACGGCAGGTGAGTGGGGACACAATCAGCTACCGTGGATGGACGAAGACGAACTGCGTTATCGCGAGGAAGTCCCTTGTTATTGCGGTAAACAAGGTTGTATTGAAACCTTTATTTCGGGCACGGGATTCGCGATGGAT                                     <  SRR19973070.1112277/150‑1 (MQ=60)
                                                                                                                GGCGGCAATGGCACGGCAGGTGAGTGGGGACACAATCAGCTACCGTGGATGGACGAAGACGAACTGCGTTATCGCGAGGAAGTCCCTTGTTATTGCGGTAAACAAGGTTGTATTGAAACCTTTATTTCGGGCACGGGATTCGCGATGGAT                                     <  SRR19973070.2416251/150‑1 (MQ=60)
                                                                                                                     CAATGGCACGGCAGGTGAGTGGGGACACAATCAGCTACCGTGGATGGACGAAGACGAACTGCGTTATCGCGAGGAAGTCCCTTGTTATTGCGGTAAACAAGGTTGTATTGAAACCTTTATTTCGGGCACGGGATTCGCGATGGATTATCG                                >  SRR19973070.541214/1‑150 (MQ=60)
                                                                                                                     CAATGGCACGGCAGGTGAGCGGGGACACAATCAGCTACCGTGGATGGACGAAGACGAACTGCGTTATCGCGAGGAAGTCCCTTGTTATTGCGGTAAACAAGGTTGTATTGAAACCTTTATTTCGGGCACGGGATTCGCGATGGATTATCG                                >  SRR19973070.541115/1‑150 (MQ=60)
                                                                                                                        TGGCACGGCAGGTGAGTGGGGACACAATCAGCTACCGTGGATGGACGAAGACGAACTGCGTTATCGCGAGGAAGTCCCTTGTTATTGCGGTAAACAAGGTTGTATTGAAACCTTTATTTCGGGCACGGGATTCGCGATGGATTATCGTCG                             >  SRR19973070.499911/1‑150 (MQ=60)
                                                                                                                         GGCACGGCAGGTGAGTGGGGACACAATCAGCTACCGTGGATGGACGAAGACGAACTGCGTTATCGCGAGGAAGTCCCTTGTTATTGCGGTAAACAAGGTTGTATTGAAACCTTTATTTCGGGCACGGGATTCGCGATGGATTATCGTCGT                            >  SRR19973070.954376/1‑150 (MQ=60)
                                                                                                                         GGCACGGCAGGTGAGTGGGGACACAATCAGCTACCGTGGATGGACGAAGACGAACTGCGTTATCGCGAGGAAGTCCCTTGTTATTGCGGTAAACAAGGTTGTATTGAAACCTTTATTTCGGGCACGGGATTCGCGATGGATTATCGTCGT                            >  SRR19973070.954263/1‑150 (MQ=60)
                                                                                                                                CAGGTGAGTGGGGACACAATCAGCTACCGTGGATGGACGAAGACGAACTGCGTTATCGCGAGGAAGTCCCTTGTTATTGCGGTAAACAAGGTTGTATTGAAACCTTTATTTCGGGCACGGGATTCGCGATGGATTATCGTCGTTTGTGCG                     >  SRR19973070.367977/1‑150 (MQ=60)
                                                                                                                                CAGGTGAGTGGGGACACAATCAGCTACCGTGGATGGACGAAGACGAACTGCGTTATCGCGAGGAAGTCCCTTGTTATTGCGGTAAACAAGGTTGTATTGAAACCTTTATTTCGGGCACGGGATTCGCGATGGATTATCGTCGTTTGAGCG                     >  SRR19973070.2476922/1‑150 (MQ=60)
                                                                                                                                CAGGTGAGTGGGGACACAATCAGCTACCGTGGATGGACGAAGACGAACTGCGTTATCGCGAGGAAGTCCCTTGTTATTGCGGTAAACAAGGTTGTATTGAAACCTTTATTTCGGGCACGGGATTCGCGATGGATTATCGTCGTTTGAGCG                     >  SRR19973070.367991/1‑150 (MQ=60)
                                                                                                                                CAGGTGAGTGGGGACACAATCAGCTACCGTGGATGGACGAAGACGAACTGCGTTATCGCGAGGAAGTCCCTTGTTATTGCGGTAAACAAGGTTGTATTGAAACCTTTATTTCGGGCACGGGATTCGCGATGGATTATCGTCGTTTGAGCG                     >  SRR19973070.1679592/1‑150 (MQ=60)
                                                                                                                                      AGTGGGGACACAATCAGCTACCGTGGATGGACGAAGACGAACTGCGTTATCGCGAGGAAGTCCCTTGTTATTGCGGTAAACAAGGTTGTATTGAAACCTTTATTTCGGGCACGGGATTCGCGATGGATTATCGTCGTTTGAGCGGACATG               <  SRR19973070.2445292/150‑1 (MQ=60)
                                                                                                                                      AGTGGGGACACAATCAGCTACCGTGGATGGACGAAGACGAACTGCGTTATCGCGAGGAAGTCCCTTGTTATTGCGGTAAACAAGGTTGTATTGAAACCTTTATTTCGGGCACGGGATTCGCGATGGATTATCGTCGTTTGAGCGGACATG               <  SRR19973070.774513/150‑1 (MQ=60)
                                                                                                                                       GTGGGGACACAATCAGCTACCGTGGATGGACGAAGACGAACTGCGTTATCGCGAGGAAGTCCCTTGTTATTGCGGTAAACAAGGTTGTATTGAAACCTTTATTTCGGGCACGGGATTCGCGATGGATTATCGTCGTTTGAGCGGACATGC              <  SRR19973070.1453574/150‑1 (MQ=60)
                                                                                                                                             ACACAATCAGCTACCGTGGATGGACGAAGACGAACTGCGTTATCGCGAGGAAGTCCCTTGTTATTGCGGTAAACAAGGTTGTATTGAAACCTTTATTTCGGGCACGGGATTCGCGATGGATTATCGTCGTTTGAGCGGACATGCGCTGAA        <  SRR19973070.1165470/150‑1 (MQ=60)
                                                                                                                                                  ATCAGCTACCGTGGATGGACGAAGACGAACTGCGTTATCGCGAGGAAGTCCCTTGTTATTGCGGTAAACAAGGTTGTATTGAAACCTTTATTTCGGGCACGGGATTCGCGATGGATTATCGTCGTTTGAGCGGACATGCGCTGAAAGGCA   >  SRR19973070.516896/1‑150 (MQ=60)
                                                                                                                                                   TCAGCTACCGTGGATGGACGAAGACGAACTGCGTTATCGCGAGGAAGTCCCTTGTTATTGCGGTAAACAAGGTTGTATTGAAACCTTTATTTCGGGCACGGGATTCGCGATGGATTATCGTCGTTTGAGCGGACATGCGCTGAAAGGCAG  >  SRR19973070.590137/1‑150 (MQ=60)
                                                                                                                                                   TCAGCTACCGTGGATGGACGAAGACGAACTGCGTTATCGCGAGGAAGTCCCTTGTTATTGCGGTAAACAAGGTTGTATTGAAACCTTTATTTCGGGCACGGGATTCGCGATGGATTATCGTCGTTTGAGCGGACATGCGCGGAAAGGCAG  >  SRR19973070.590291/1‑150 (MQ=60)
                                                                                                                                                     |                                                                                                                                                   
GGCGGTTTCAGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATCGGCACGGGATGCGGCGCGGGCGTGGCATTCAATGGGCGGGCGCATATCGGCGGCAATGGCACGGCAGGTGAGTGGGGACACAATCCGCTACCGTGGATGGACGAAGACGAACTGCGTTATCGCGAGGAAGTCCCTTGTTATTGCGGTAAACAAGGTTGTATTGAAACCTTTATTTCGGGCACGGGATTCGCGATGGATTATCGTCGTTTGAGCGGACATGCGCTGAAAGGCAG  >  CP009273/405926‑406222

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑