Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F309 I0 R1 1697 197.2 6627756 95.7% 6342762 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,813,263 C→A 12.9% E148* (GAG→TAG)  chbR ← repressor of chb operon for N,N'‑diacetylchitobiose utilization

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,813,2630CA12.9% 708.5 / 54.1 216E148* (GAG→TAG) chbRrepressor of chb operon for N,N'‑diacetylchitobiose utilization
Reads supporting (aligned to +/- strand):  ref base C (89/99);  new base A (16/12);  total (105/111)
Fisher's exact test for biased strand distribution p-value = 4.18e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 227 total aligned reads displayed.

ACCATATTCTCCAGCGCCGATTCACTAAACTGCTCTTTATCATGCATCTTTTCTACCGTACTTTTCAGCCACTGCGGTACATCATCAATCACCTGTTCTTCGCGGTAATGACGTAAACGGTTAATGACATAAAAAGTAACCATCTCAACAAACTCTTCCAGCCCTGTTTCGCGGAAATTCAATGAAGAAATCACTGTTTCCACATAGGTGAGAAAAGCGTTATTGGTCCGGTATACCTGCGAAGCGACAAAGCAATAAGGCAACAATGGCAGGTAATGCTGCTCAAAAAAGCG  >  CP009273/1813118‑1813410
                                                                                                                                                 |                                                                                                                                                   
accaTATTCTCCAGCGCCGATTCACTAAACTGCTCTTTATCATGCATCTTTTCTACCGTACTTTTCAGCCACTGCGGTACATCATCAATCACCTGTTCTTCGCGGTAATGACGTAAACGGTTAATGACATAAAAAGTAACCATCTcaa                                                                                                                                                   <  1:1299057/148‑1 (MQ=255)
accaTATTCTCCAGCGCCGATTCACTAAACTGCTCTTTATCATGCATCTTTTCTACCGTACTTTTCAGCCACTGCGGTACATCATCAATCACCTGTTCTTCGCGGTAATGACGTAAACGGTTAATGACATAAAAAGTAACCATCTcaa                                                                                                                                                   >  1:49417/1‑148 (MQ=255)
 ccaTATTCTCCAGCGCCGATTCACTAAACTGCTCTTTATCATGCATCTTTTCTACCGTACTTTTCAGCCACTGCGGTACATCATCAATCACCTGTTCTTCGCGGTAATGACGTAAACGGTTAATGACATAAAAAGTAACCATCTcaac                                                                                                                                                  >  1:3229974/1‑148 (MQ=255)
   atatTCTCCAGCGCCGATTCACTAAACTGCTCTTTATCATGCATCTTTTCTACCGTACTTTTCAGCCACTGCGGTACATCATCAATCACCTGTTCTTCGCGGTAATGACGTAAACGGTTAATGACATAAAAAGTAACCATCTcaacaa                                                                                                                                                <  1:2789263/148‑1 (MQ=255)
    tatTCTCCAGCGCCGATTCACTAAACTGCTCTTTATCATGCATCTTTTCTACCGTACTTTTCAGCCACTGCGGTACATCATCAATCACCTGTTCTTCGCGGTAATGACGTAAACGGTTAATGACATAAAAAGTAACCATCTCAACaaa                                                                                                                                               >  1:1903751/1‑148 (MQ=255)
     atTCTCCAGCGCCGATTCACTAAACTGCTCTTTATCATGCATCTTTTCTACCGTACTTTTCAGCCACTGCGGTACATCATCAATCACCTGTTCTTCGCGGTAATGACGTAAACGGTTAATGACATAAAAAGTAACCATCTCAACAAAc                                                                                                                                              >  2:1640797/1‑148 (MQ=255)
     atTCTCCAGCGCCGATTCACTAAACTGCTCTTTATCATGCATCTTTTCTACCGTACTTTTCAGCCACTGCGGTACATCATCAATCACCTGTTCTTCGCGGTAATGACGTAAACGGTTAATGACATAAAAAGTAACCATCTCAACAAAc                                                                                                                                              >  2:2751666/1‑148 (MQ=255)
      ttCTCCAGCGCCGATTCACTAAACTGCTCTTTATGATGCATCTTTTCTACCGTACTTTTCAGCCACTGCGGTACATCATCAATCACCTGTTCTTCGCGGTAATGACGTAAACGGTTAATGACATAAAAAGTAACCATCTAAACAAAct                                                                                                                                             >  1:488917/1‑148 (MQ=255)
      ttCTCCAGCGCCGATTCACTAAACTGCTCTTTATCATGCATCTTTTCTACCGTACTTTTCAGCCACTGCGGTACATCATCAATCACCTGTTCTTCGCGGTAATGACGTAAACGGTTAATGACATAAAAAGTAACCATCTCAACAAAct                                                                                                                                             >  2:1771865/1‑148 (MQ=255)
       tctcCAGCGCCGATTCACTAAACTGCTCTTTATCATGCATCTTTTCTACCGTACTTTTCAGCCACTGCGGTACATCATCAATCACCTGTTCTTCGCGGTAATGACGTAAACGGTTAATGACATAAAAAGTAACCATCTCAACAAActc                                                                                                                                            >  2:1007511/1‑148 (MQ=255)
          ccAGCGCCGATTCACTAAACTGCTCTTTATCATGCATCTTTTCTACCGTACTTTTCAGCCACTGCGGTACATCATCAATCACCTGTTCTTCGCGGTAATGACGTAAACGGTTAATGACATAAAAAGTAACCATCTCAACAAACTCTTc                                                                                                                                         <  2:520194/148‑1 (MQ=255)
              cgcCGATTCACTAAACTGCTCTTTATCATGCATCTTTTCTACCGTACTTTTCAGCCACTGCGGTACATCATCAATCACCTGTTCTTCGCGGTAATGACGTAAACGGTTAATGACATAAAAAGTAACCATCTCAACAAACTCTTCCAGc                                                                                                                                     >  2:9085/1‑148 (MQ=255)
                 cGATTCACTAAACTGCTCTTTATCATGCATCTTTTCTACCGTACTTTTCAGCCACTGCGGTACATCATCAATCACCTGTTCTTCGCGGTAATGACGTAAACGGTTAATGACATAAAAAGTAACCATCTCAACAAACTCTTCCAGCCCt                                                                                                                                  <  2:2915034/148‑1 (MQ=255)
                   aTTCACTAAACTGCTCTTTATCATGCATCTTTTCTACCGTACTTTTCAGCCACTGCGGTACATCATCAATCACCTGTTCTTCGCGGTAATGACGTAAACGGTTAATGACATAAAAAGTAACCATCTAAACAAACTCTTCCAGCCCTGt                                                                                                                                <  2:415367/148‑1 (MQ=255)
                     tCACTAAACTGCTCTTTATCATGCATCTTTTCTACCGTACTTTTCAGCCACTGCGGTACATCATCAATCACCTGTTCTTCGCGGTAATGACGTAAACGGTTAATGACATAAAAAGTAACCATCTCAACAAACTCTTCCAGCCCTGttt                                                                                                                              >  1:1575636/1‑148 (MQ=255)
                     tCACTAAACTGCTCTTTATCATGCATCTTTTCTAACGTACTTTTCAGCCACTGCGGTACAACAACAATCACCTGTTCTTCGCGGTAATGACGTAAACGGTTAATGACATACAAAGTAACCATCTCAACAAACTCTTCCAGCCCTGttt                                                                                                                              >  2:2209660/1‑148 (MQ=255)
                         tAAACTGCTCTTTATCATGCATCTTTTCTACCGTACTTTTCAGCCACTGCGGTACATCATCAATCACCTGTTCTTCGCGGTAATGACGTAAACGGTTAATGACATAAAAAGTAACCATCTCAACAAACTCTTCCAGCCCTGTTTcgcg                                                                                                                          >  1:2801152/1‑148 (MQ=255)
                          aaaCTGCTCTTTATCATGCATCTTTTCTACCGTACTTTTCAGCCACTGCGGTACATCATCAATCACCTGTTCTTCGCGGTAATGACGTAAACGGTTAATGACATAAAAAGTAACCATCTCAACAAACTCTTCCAGCCCTGTTTCGCgg                                                                                                                         >  1:952927/1‑148 (MQ=255)
                           aaCTGCTCTTTATCATGCATCTTTTCTACCGTACTTTTCAGCCACTGCGGTACATCATCAATCACCTGTTCTTCGCGGTAATGACGTAAACGGTTAATGACATAAAAAGTAACCATCTCAACAAACTCTTCCAGCCCTGTTTCGCGGa                                                                                                                        >  2:1885079/1‑148 (MQ=255)
                             cTGCTCTTTATCATGCATCTTTTCTACCGTACTTTTCAGCCACTGCGGTACATCATCAATCACCTGTTCTTCGCGGTAATGACGTAAACGGTTAATGACATAAAAAGTAACCATCTCAACAAACTCTTCCAGCCCTGTTTCGCGGaaa                                                                                                                      >  2:120158/1‑148 (MQ=255)
                             cTGCTCTTTATCATGCATCTTTTCTACCGTACTTTTCAGCCACTGCGGTACATCATCAATCACCTGTTCTTCGCGGTAATGACGTAAACGGTTAATGACATAAAAAGTAACCATCTCAACAAACTCTTCCAGCCCTGTTTCGCGGaaa                                                                                                                      <  1:3272533/148‑1 (MQ=255)
                             cTGCTCTTTATCATGCATCTTTTCTACCGTACTTTTCAGCCACTGCGGTACATCATCAATCACCTGTTCTTCGCGGTAATGACGTAAACGGTTAATGACATAAAAAGTAACCATCTCAACAAACTCTTCCAGCCCTGTTTCGCGGaaa                                                                                                                      <  1:1356339/148‑1 (MQ=255)
                              tGCTCTTTATCATGCATCTTTTCTACCGTACTTTTCAGCCACTGCGGTACATCATCAATCACCTGTTCTTCGCGGTAATGACGTAAACGGTTAATGACATAAAAAGTAACCATCTCAACAAACTCTTCCAGCCCTGTTTCGCGGAAAt                                                                                                                     <  1:969671/148‑1 (MQ=255)
                               gCTCTTTATCATGCATCTTTTCTACCGTACTTTTCAGCCACTGCGGTACATCATCAATCACCTGTTCTTCGCGGTAATGACGTAAACGGTTAATGACATAAAAAGTAACCATCTCAACAAACTCTTCCAGCCCTGTTTCGCGGAAAtt                                                                                                                    >  2:1403907/1‑148 (MQ=255)
                                  ctTTATCATGCATCTTTTCTACCGTACTTTTCAGCCACTGCGGTACATCATCAATCACCTGTTCTTCGCGGTAATGACGTAAACGGTTAATGACATAAAAAGTAACCATCTCAACAAACTCTTCCAGCCCTGTTTCGCGGAAATTCaa                                                                                                                 <  1:1996727/148‑1 (MQ=255)
                                  ctTTATCATGCATCTTTTCTACCGTACTTTTCAGCCACTGCGGTACATCATCAATCACCTGTTCTTCGCGGTAATGACGTAAACGGTTAATGACATAAAAAGTAACCATCTCAACAAACTCTTCCAGCCCTGTTTCGCGGAAATTCaa                                                                                                                 >  2:1288572/1‑148 (MQ=255)
                                   tttATCATGCATCTTTTCTACCGTACTTTTCAGCCACTGCGGTACATCATCAATCACCTGTTCTTCGCGGTAATGACGTAAACGGTTAATGACATAAAAAGTAACCATCTCAACAAACTCTTCCAGCCCTGTTTCGCGGAAATTCAAt                                                                                                                <  1:2360286/148‑1 (MQ=255)
                                       tCATGCATCTTTTCTACCGTACTTTTCAGCCACTGCGGTACATCATCAATCACCTGTTCTTCGCGGTAATGACGTAAACGGTTAATGACATAAAAAGTAACCATCTCAACAAACTCTTCCAGCCCTGTTTCGCGGAAATTCAATgaag                                                                                                            >  2:3146342/1‑148 (MQ=255)
                                        cATGCATCTTTTCTACCGTACTTTTCAGCCACTGCGGTACATCATCAATCACCTGTTCTTCGCGGTAATGACGTAAACGGTTAATGACATAAAAAGTAACCATCTAAACAAACTCTTCCAGCCCTGTTTCGCGGAAATTCAATgaaga                                                                                                           >  1:1081291/1‑148 (MQ=255)
                                          tGCATCTTTTCTACCGTACTTTTCAGCCACTGCGGTACATCATCAATCACCTGTTCTTCGCGGTAATGACGTAAACGGTTAATGACATAAAAAGTAACCATCTCAACAAACTCTTCCAGCCCTGTTTCGCGGAAATTCAATGAAGaaa                                                                                                         <  1:3077097/148‑1 (MQ=255)
                                          tGCATCTTTTCTACCGTACTTTTCAGCCACTGCGGTACATCATCAATCACCTGTTCTTCGCGGTAATGACGTAAACGGTTAATGACATAAAAAGTAACCATCTCAACAAACTCTTCCAGCCCTGTTTCGCGGAAATTCAATGAAGaaa                                                                                                         >  1:37556/1‑148 (MQ=255)
                                           gCATCTTTTCTACCGTACTTTTCAGCCACTGCGGTACATCATCAATCACCTGTTCTTCGCGGTAATGACGTAAACGGTTAATGACATAAAAAGTAACCATCTAAACAAACTCTTCCAGCCCTGTTTCGCGGAAATTCAATGAAGAAAt                                                                                                        >  2:2976749/1‑148 (MQ=255)
                                            cATCTTTTCTACCGTACTTTTCAGCCACTGCGGTACATCATCAATCACCTGTTCTTCGCGGTAATGACGTAAACGGTTAATGACATAAAAAGTAACCATCTCAACAAACTCTTCCAGCCCTGTTTCGCGGAAATTCAATGAAGAAATc                                                                                                       <  2:1725551/148‑1 (MQ=255)
                                             aTCTTTTCTACCGTACTTTTCAGCCACTGCGGTACATCATCAATCACCTGTTCTTCGCGGTAATGACGTAAACGGTTAATGACATAAAAAGTAACCATCTCAACAAACTCTTCCAGCCCTGTTTCGCGGAAATTCAATGAAGAAATCa                                                                                                      <  1:1471480/148‑1 (MQ=255)
                                              tCTTTTCTACCGTACTTTTCAGCCACTGCGGTACATCATCAATCACCTGTTCTTCGCGGTAATGACGTAAACGGTTAATGACATAAAAAGTAACCATCTCAACAAACTCTTCCAGCCCTGTTTCGCGGAAATTCAATGAAGAAATCAc                                                                                                     >  2:535361/1‑148 (MQ=255)
                                              tCTTTTCTACCGTACTTTTCAGCCACTGCGGTACATCATCAATCACCTGTTCTTCGCGGTAATGACGTAAACGGTTAATGACATAAAAAGTAACCATCTAAACAAACTCTTCCAGCCCTGTTTCGCGGAAATTCAATGAAGAAATCAc                                                                                                     >  1:2242668/1‑148 (MQ=255)
                                                ttttCTACCGTACTTTTCAGCCACTGCGGTACATCATCAATCACCTGTTCTTCGCGGTAATGACGTAAACGGTTAATGACATAAAAAGTAACCATCTCAACAAACTCTTCCAGCCCTGTTTCGCGGAAATTCAATGAAGAAATCACTg                                                                                                   <  1:457725/148‑1 (MQ=255)
                                                  ttCTACCGTACTTTTCAGCCACTGCGGTACATCATCAATCACCTGTTCTTCGCGGTAATGACGTAAACGGTTAATGACATAAAAAGTAACCATCTCAACAAACTCTTCCAGCCCTGTTTCGCGGAAATTCAATGAAGGAATCACTGtt                                                                                                 <  1:3088943/148‑1 (MQ=255)
                                                  ttCTACCGTACTTTTCAGCCACTGCGGTACATCATCAATCACCTGTTCTTCGCGGTAATGACGTAAACGGTTAATGACATAAAAAGTAACCATCTCAACAAACTCTTCCAGCCCTGTTTCGCGGAAATTCAATGAAGAAATCACTGtt                                                                                                 <  2:2482626/148‑1 (MQ=255)
                                                   tCTACCGTACTTTTCAGCCACTGCGGTACATCATCAATCACCTGTTCTTCGCGGTAATGACGTAAACGGTTAATGACATAAAAAGTAACCATCTCAACAAACTCTTCCAGCCCTGTTTCGCGGAAATTCAATGAAGAAATCACTGttt                                                                                                >  2:2596233/1‑148 (MQ=255)
                                                   tCTACCGTACTTTTCAGCCACTGCGGTACATCATCAATCACCTGTTCTTCGCGGTAATGACGTAAACGGTTAATGACATAAAAAGTAACCATCTCAACAAACTCTTCCAGCCCTGTTTCGCGGAAATTCAATGAAGAAATCACTGttt                                                                                                <  1:300052/148‑1 (MQ=255)
                                                        cGTACTTTTCAGCCACTGCGGTACATCATCAATCACCTGTTCTTCGCGGTAATGACGTAAACGGTTAATGACATAAAAAGTAACCATCTCAACAAACTCTTCCAGCCCTGTTTCGCGGAAATTCAATGAAGAAATCACTGTTTCcaca                                                                                           >  1:1862579/1‑148 (MQ=255)
                                                            ctgttCAGCCACTGCGGTACATCATCAATCACCTGTTCTTCGCGGTAATGACGTAAACGGTTAATGACATAAAAAGTAACCATCTCAACAAACTCTTCCAGCCCTGTTTCGCGGAAATTCAATGAAGAAATCACTGTTTCCACATAgg                                                                                       <  1:2284969/145‑1 (MQ=255)
                                                                 cAGCCACTGCGGTACATCATCAATCACCTGTTCTTCGCGGTAATGACGTAAACGGTTAATGACATAAAAAGTAACCATCTCAACAAACTCTTCCAGCCCTGTTTCGCGGAAATTCAATGAAGAAATCACTGTTTCCACATAGGTgaga                                                                                  <  1:2688764/148‑1 (MQ=255)
                                                                 cAGCCACTGCGGTACATCATCAATCACCTGTTCTTCGCGGTAATGACGTAAACGGTTAATGACATAAAAAGTAACCATCTCAACAAACTCTTCCAGCCCTGTTTCGCGGAAATTCAATGAAGAAATCACTGTTTCCACATAGGTgaga                                                                                  <  2:2350994/148‑1 (MQ=255)
                                                                 cAGCCACTGCGGTACATCATCAATCACCTGTTCTTCGCGGTAATGACGTAAACGGTTAATGACATAAAAAGTAACCATCTCAACAAACTCTTCCAGCCCTGTTTCGCGGAAATTCAATGAAGAAATCACTGTTTCCACATAGGTgaga                                                                                  <  2:690487/148‑1 (MQ=255)
                                                                   gCCACTGCTGTACATCATCAATCACCTGTTCTTCGCGGTAATGACGTAAACGGTTAATGACATAAAAAGTAACCATCTCAACAAACTCTTCCAGCCCTGTTTCGCGGAAATTCAATGAAGAAATCACTGTTTCCACATAGGTGAGaaa                                                                                <  1:1709452/148‑1 (MQ=255)
                                                                       cTGCGGTACATCATCAATCACCTGTTCTTCGCGGTAATGACGTAAACGGTTAATGACATAAAAAGTAACCATCTCAACAAACTCTTCCAGCCCTGTTTCGCGGAAATTCAATGAAGAAATCACTGTTTCCACATAGGTGAGAAAAGCg                                                                            <  2:36171/148‑1 (MQ=255)
                                                                       cTGCGGTACATCATCAATCACCTGTTCTTCGCGGTAATGACGTAAACGGTTAATGACATAAAAAGTAACCATCTCAACAAACTCTTCCAGCCCTGTTTCGCGGAAATTCAATGAAGAAATCACTGTTTCCACATAGGTGAGAAAAGCg                                                                            <  2:2043160/148‑1 (MQ=255)
                                                                        tGCGGTACATCATCAATCACCTGTTCTTCGCGGTAATGACGTAAACGGTTAATGACATAAAAAGTAACCATCTAAACAAACTCTTCCAGCCCTGTTTCGCGGAAATTCAATGAAGAAATCACTGTTTCCACATAGGTGAGAAAAGCGt                                                                           >  1:286471/1‑148 (MQ=255)
                                                                         gCGGTACATCATCAATCACCTGTTCTTCGCGGTAATGACGTAAACGGTTAATGACATAAAAAGTAACCATCTCAACAAACTCTTCCAGCCCTGTTTCGCGGAAATTCAATGAAGAAATCACTGTTTCCACATAGGTGAGAAAAGCGtt                                                                          <  2:638902/148‑1 (MQ=255)
                                                                          cGGTACATCATCAATCACCTGTTCTTCGCGGTAATGACGTAAACGGTTAATGACATAAAAAGTAACCATCTCAACAAACTCTTCCAGCCCTGTTTCGCGGAAATTCAATGAAGAAATCACTGTTTCCACATAGGTGAGAAAAGCGTTa                                                                         <  1:670437/148‑1 (MQ=255)
                                                                          cGGTACATCATCAATCACCTGTTCTTCGCGGTAATGACGTAAACGGTTAATGACATAAAAAGTAACCATCTCAACAAACTCTTCCAGCCCTGTTTCGCGGAAATTCAATGAAGAAATCACTGTTTCCACATAGGTGAGAAAAGCGTTa                                                                         <  1:1125358/148‑1 (MQ=255)
                                                                           ggTACATCATCAATCACCTGTTCTTCGCGGTAATGACGTAAACGGTTAATGACATAAAAAGTAACCATCTCAACAAACTCTTCCAGCCCTGTTTCGCGGAAATTCAATGAAGAAATCACTGTTTCCACATAGGTGAGAAAAGCGTTAt                                                                        <  2:2872700/148‑1 (MQ=255)
                                                                             tACATCATCAATCACCTGTTCTTCGCGGTAATGACGTAAACGGTTAATGACATAAAAAGTAACCATCTCAACAAACTCTTCCAGCCCTGTTTCGCGGAAATTCAATGAAGAAATCACTGTTTCCACATAGGTGAGAAAAGCGTTATTg                                                                      <  2:88227/148‑1 (MQ=255)
                                                                              aCATCATCAATCACCTGTTCTTCGCGGTAATGACGTAAACGGTTAATGACATAAAAAGTAACCATCTCAACAAACTCTTCCAGCCCTGTTTCGCGGAAATTCAATGAAGAAATCACTGTTTCCACATAGGTGAGAAAAGCGTTATTgg                                                                     <  2:1802343/148‑1 (MQ=255)
                                                                              aCATCATCAATCACCTGTTCTTCGCGGTAATGACGTAAACGGTTAATGACATAAAAAGTAACCATCTCAACAAACTCTTCCAGCCCTGTTTCGCGGAAATTCAATGAAGAAATCACTGTTTCCACATAGGTGAGAAAAGCGTTATTgg                                                                     <  2:97694/148‑1 (MQ=255)
                                                                                  catcaATCACCTGTTCTTCGCGGTAATGACGTAAACGGTTAATGACATAAAAAGTAACCATCTCAACAAACTCTTCCAGCCCTGTTTCGCGGAAATTCAATGAAGAAATCACTGTTTCCACATAGGTGAGAAAAGCGTTATTGGTCCg                                                                 >  2:3298855/1‑148 (MQ=255)
                                                                                  catcaATCACCTGTTCTTCGCGGTAATGACGTAAACGGTTAATGACATAAAAAGTAACCATCTCAACAAACTCTTCCAGCCCTGTTTCGCGGAAATTCAATGAAGAAATCACTGTTTCCACATAGGTGAGAAAAGCGTTATTGGTCCg                                                                 <  1:2436355/148‑1 (MQ=255)
                                                                                  catcaATCACCTGTTCTTCGCGGTAATGACGTAAACGGTTAATGACATAAAAAGTAACCATCTCAACAAACTCTTCCAGCCCTGTTTCGCGGAAATTCAATGAAGAAATCACTGTTTCCACATAGGTGAGAAAAGCGTTATTGGTCCg                                                                 >  2:2446019/1‑148 (MQ=255)
                                                                                    tcaatcaCCTGTTCTTCGCGGTAATGACGTAAACGGTTAATGACATAAAAAGTAACCATCTCAACAAACTCTTCCAGCCCTGTTTCGCGGAAATTCAATGAAGAAATCACTGTTTCCACATAGGTGAGAAAAGCGTTATTGGTCCGGt                                                               >  2:1555530/1‑148 (MQ=255)
                                                                                     caatcaCCTGTTCTTCGCGGTAATGACGTAAACGGTTAATGACATAAAAAGTAACCATCTCAACAAACTCTTCCAGCCCTGTTTCGCGGAAATTCAATGAAGAAATCACTGTTTCCACATAGGTGAGAAAAGCGTTATTGGTCCGGta                                                              >  1:2283202/1‑148 (MQ=255)
                                                                                       atcaCCTGTTCTTCGCGGTAATGACGTAAACGGTTAATGACATAAAAAGTAACCATCTCAACAAACTCTTCCAGCCCTGTTTCGCGGAAATTCAATGAAGAAATCACTGTTTCCACATAGGTGAGAAAAGCGTTATTGGTCCGGtata                                                            >  2:2228001/1‑148 (MQ=255)
                                                                                        tcaCCTGTTCTTCGCGGTAATGACGTAAACGGTTAATGACATAAAAAGTAACCATCTCAACAAACTCTTCCAGCCCTGTTTCGCGGAAATTCAATGAAGAAATCACTGTTTCCACATAGGTGAGAAAAGCGTTATTGGTCCGGTATAc                                                           >  1:1024762/1‑148 (MQ=255)
                                                                                          aCCTGTTCTTCGCGGTAATGACGTAAACGGTTAATGACATAAAAAGTAACCATCTCAACAAACTCTTCCAGCCCTGTTTCGCGGAAATTCAATGAAGAAATCACTGTTTCCACATAGGTGAGAAAAGCGTTATTGGTCCGGTATACCt                                                         >  2:2431691/1‑148 (MQ=255)
                                                                                           ccTGTTCTTCGCGGTAATGACGTAAACGGTTAATGACATAAAAAGTAACCATCTCAACAAACTCTTCCAGCCCTGTTTCGCGGAAATTCAATGAAGAAATCACTGTTTCCACATAGGTGAGAAAAGCGTTATTGGTCCGGTATACCTg                                                        >  2:3093514/1‑148 (MQ=255)
                                                                                            cTGTTCTTCGCGGTAATGACGTAAACGGTTAATGACATAAAAAGTAACCATCTCAACAAACTCTTCCAGCCCTGTTTCGCGGAAATTCAATGAAGAAATCACTGTTTCCACATAGGTGAGAAAAGCGTTATTGGTCCGGTATACCTGc                                                       >  2:968002/1‑148 (MQ=255)
                                                                                               ttcttcGCGGTAATGACGTAAACGGTTAATGACATAAAAAGTAACCATCTCAACAAACTCTTCCAGCCCTGTTTCGCGGAAATTCAATGAAGAAATCACTGTTTCCACATAGGTGAGAAAAGCGTTATTGGTCCGGTATACCTGCGaa                                                    >  2:3201332/1‑148 (MQ=255)
                                                                                                 cttcGCGGTAATGACGTAAACGGTTAATGACATAAAAAGTAACCATCTCAACAAACTCTTCCAGCCCTGTTTCGCGGAAATTCAATGAAGAAATCACTGTTTCCACATAGGTGAGAAAAGCGTTATTGGTCCGGTATACCTGCGAAGc                                                  >  2:1164561/1‑148 (MQ=255)
                                                                                                 cttcGCGGTAATGACGTAAACGGTTAATGACATAAAAAGTAACCATCTAAACAAACTCTTCCAGCCCTGTTTCGCGGAAATTCAATGAAGAAATCACTGTTTCCACATAGGTGAGAAAAGCGTTATTGGTCCGGTATACCTGCGAAGc                                                  >  2:1684129/1‑148 (MQ=255)
                                                                                                          aaTGACGTAAACGGTTAATGCCATAAAAAGTAACCATCTAAACAAACTCTTCCAGCCCTGTTTCGCGGAAATTCAATGAAGAAATCACTGTTTCCACATAGGTGAGAAAAGCGTTATTGGTCCGGTATACCTGCGAAGCGACAAAGCa                                         <  1:2018919/148‑1 (MQ=255)
                                                                                                          aaTGACGTAAACGGTTAATGACATAAAAAGTAACCATCTAAACAAACTCTTCCAGCCCTGTTTCGCGGAAATTCAATGAAGAAATCACTGTTTCCACATAGGTGAGAAAAGCGTTATTGGTCCGGTATACCTGCGAAGCGACAAAGCa                                         <  1:2019247/148‑1 (MQ=255)
                                                                                                            tGACGTAAACGGTTAATGACATAAAAAGTAACCATCTCAACAAACTCTTCCAGCCCTGTTTCGCGGAAATTCAATGAAGAAATCACTGTTTCCACATAGGTGAGAAAAGCGTTATTGGTCCGGTATACCTGCGAAGCGACAAAGCAAt                                       >  1:1252038/1‑148 (MQ=255)
                                                                                                               cGTAAACGGTTAATGACATAAAAAGTAACCATCTCAACAAACTCTTCCAGCCCTGTTTCGCGGAAATTCAATGAAGAAATCACTGTTTCCACATAGGTGAGAAAAGCGTTATTGGTCCGGTATACCTGCGAAGCGACAAAGCAATAAg                                    >  2:1855618/1‑148 (MQ=255)
                                                                                                                  aaaCGGTTAATGACATAAAAAGTAACCATCTCAACAAACTCTTCCAGCCCTGTTTCGCGGAAATTCAATGAAGAAATCACTGTTTCCACATAGGTGAGAAAAGCGTTATTGGTCCGGTATACCTGCGAAGCGACAAAGCAATAAGGca                                 <  1:2853132/148‑1 (MQ=255)
                                                                                                                  aaaCGGTTAATGACATAAAAAGTAACCATCTAAACAAACTCTTCCAGCCCTGTTTCGCGGAAATTCAATGAAGAAATCACTGTTTCCACATAGGTGAGAAAAGCGTTATTGGTCCGGTATACCTGCGAAGCGACAAAGCAATAAGGca                                 >  1:2896392/1‑148 (MQ=255)
                                                                                                                   aaCGGTTAATGACATAAAAAGTAACCATCTCAACAAACTCTTCCAGCCCTGTTTCGCGGAAATTCAATGAAGAAATCACTGTTTCCACATAGGTGAGAAAAGCGTTATTGGTCCGGTATACCTGCGAAGCGACAAAGCAATAAGGcaa                                <  1:535437/148‑1 (MQ=255)
                                                                                                                    aCGGTTAATGACATAAAAAGTAACCATCTCAACAAACTCTTCCAGCCCTGTTTCGCGGAAATTCAATGAAGAAATCACTGTTTCCACATAGGTGAGAAAAGCGTTATTGGTCCGGTATACCTGCGAAGCGACAAAGCAATAAGGcaac                               >  2:1758920/1‑148 (MQ=255)
                                                                                                                    aCGGTTAATGACATAAAAAGTAACCATCTCAACAAACTCTTCCAGCCCTGTTTCGCGGAAATTCAATGAAGAAATCACTGTTTCCACATAGGTGAGAAAAGCGTTATTGGTCCGGTATACCTGCGAAGCGACAAAGCAATAAGGcaac                               >  2:311566/1‑148 (MQ=255)
                                                                                                                    aCGGTTAATGACATAAAAAGTAACCATCTCAACAAACTCTTCCAGCCCTGTTTCGCGGAAATTCAATGAAGAAATCACTGTTTCCACATAGGTGAGAAAAGCGTTATTGGTCCGGTATACCTGCGAAGCGACAAAGCAATAAGGcaac                               <  2:734695/148‑1 (MQ=255)
                                                                                                                    aCGGTTAATGACATAAAAAGTAACCATCTCAACAAACTCTTCCAGCCCTGTTTCGCGGAAATTCAATGAAGAAATCACTGTTTCCACATAGGTGAGAAAAGCGTTATTGGTCCGGTATACCTGCGAAGCGACAAAGCAATAAGGcaac                               >  1:1488557/1‑148 (MQ=255)
                                                                                                                       gTTAATGACATAAAAAGTAACCATCTAAACAAACTCTTCCAGCCCTGTTTCGCGGAAATTCAATGAAGAAATCACTGTTTCCACATAGGTGAGAAAAGCGTTATTGGTCCGGTATACCTGCGAAGCGACAAAGCAATAAGGCAACAAt                            <  2:2242668/148‑1 (MQ=255)
                                                                                                                        ttAATGACATAAAAAGTAACCATCTCAACAAACTCTTCCAGCCCTGTTTCGCGGAAATTCAATGAAGAAATCACTGTTTCCACATAGGTGAGAAAAGCGTTATTGGTCCGGTATACCTGCGAAGCGACAAAGCAATAAGGCAACAATg                           >  2:1509217/1‑148 (MQ=255)
                                                                                                                        ttAATGACATAAAAAGTAACCATCTCAACAAACTCTTCCAGCCCTGTTTCGCGGAAATTCAATGAAGAAATCACTGTTTCCACATAGGTGAGAAAAGCGTTATTGGTCCGGTATACCTGCGAAGCGACAAAGCAATAAGGCAACAATg                           <  1:9085/148‑1 (MQ=255)
                                                                                                                        ttAATGACATAAAAAGTAACCATCTCAACAAACTCTTCCAGCCCTGTTTCGCGGAAATTCAATGAAGAAATCACTGTTTCCACATAGGTGAGAAAAGCGTTATTGGTCCGGTATACCTGCGAAGCGACAAAGCAATAAGGCAACAATg                           >  1:1739471/1‑148 (MQ=255)
                                                                                                                          aaTGACATAAAAAGTAACCATCTCAACAAACTCTTCCAGCCCTGTTTCGCGGAAATTCAATGAAGAAATCACTGTTTCCACATAGGTGAGAAAAGCGTTATTGGTCCGGTATACCTGCGAAGCGACAAAGCAATAAGGCAACAATGGc                         <  2:2889087/148‑1 (MQ=255)
                                                                                                                           aTGACATAAAAAGTAACCATCTCAACAAACTCTTCCAGCCCTGTTTCGCGGAAATTCAATGAAGAAATCACTGTTTCCACATAGGTGAGAAAAGCGTTATTGGTCCGGTATACCTGCGAAGCGACAAAGCAATAAGGCAACAATGGCa                        <  2:1983630/148‑1 (MQ=255)
                                                                                                                            tGACATAAAAAGTAACCATCTCAACAAACTCTTCCAGCCCTGTTTCGCGGAAATTCAATGAAGAAATCACTGTTTCCACATAGGTGAGAAAAGCGTTATTGGTCCGGTATACCTGCGAAGCGACAAAGCAATAAGGCAACAATg                           <  1:986482/144‑1 (MQ=255)
                                                                                                                              aCATAAAAAGTAACCATCTCAACAAACTCTTCCAGCCCTGTTTCGCGGAAATTCAATGAAGAAATCACTGTTTCCACATAGGTGAGAAAAGCGTTATTGGTCCGGTATACCTGCGAAGCGACAAAGCAATAAGGCAACAATGGCAGGt                     <  1:3110623/148‑1 (MQ=255)
                                                                                                                               cATAAAAAGTAACCATCTCAACAAACTCTTCCAGCCCTGTTTCGCGGAAATTCAATGAAGAAATCACTGTTTCCACATAGGTGAGAAAAGCGTTATTGGTCCGGTATACCTGCGAAGCGACAAAGCAATAAGGCAACAATGGCAGGTa                    >  1:2549890/1‑148 (MQ=255)
                                                                                                                                 tAAAAAGTAACCATCTCAACAAACTCTTCCAGCCCTGTTTCGCGGAAATTCAATGAAGAAATCACTGTTTCCACATAGGTGAGAAAAGCGTTATTGGTCCGGTATACCTGCGAAGCGACAAAGCAATAAGGCAACAATGGCAGGTAAt                  >  1:81381/1‑148 (MQ=255)
                                                                                                                                  aaaaaGTAACCATCTCAACAAACTCTTCCAGCCCTGTTTCGCGGAAATTCAATGAAGAAATCACTGTTTCCACATAGGTGAGAAAAGCGTTATTGGTCCGGTATACCTGCGAAGCGACAAAGCAATAAGGCAACAATGGCAGGTAAtg                 >  2:1625165/1‑148 (MQ=255)
                                                                                                                                  aaaaaGTAACCATCTCAACAAACTCTTCCAGCCCTGTTTCGCGGAAATTCAATGAAGAAATCACTGTTTCCACATAGGTGAGAAAAGCGTTATTGGTCCGGTATACCTGCGAAGCGACAAAGCAATAAGGCAACAATGGCAGGTAAtg                 >  2:541201/1‑148 (MQ=255)
                                                                                                                                  aaaaaGTAACCATCTCAACAAACTCTTCCAGCCCTGTTTCGCGGAAATTCAATGAAGAAATCACTGTTTCCACATAGGTGAGAAAAGCGTTATTGGTCCGGTATACCTGCGAAGCGACAAAGCAATAAGGCAACAATGGCAGGTAAtg                 <  1:2170253/148‑1 (MQ=255)
                                                                                                                                      aGTAACCATCTAAACAAACTCTTCCAGCCCTGTTTCGCGGAAATTCAATGAAGAAATCACTGTTTCCACATAGGTGAGAAAAGCGTTATTGGTCCGGTATACCTGCGAAGCGACAAAGCAATAAGGCAACAATGGCAGGTAAtgctgc             >  1:1321486/1‑148 (MQ=255)
                                                                                                                                          aCCATCTCAACAAACTCTTCCAGCCCTGTTTCGCGGAAATTCAATGAAGAAATCACTGTTTCCACATAGGTGAGAAAAGCGTTATTGGTCCGGTATACCTGCGAAGCGACAAAGCAATAAGGCAACAATGGCAGGTAATGCTGCTCaa         >  2:1630234/1‑148 (MQ=255)
                                                                                                                                             aTCTCAACAAACTCTTCCAGCCCTGTTTCGCGGAAATTCAATGAAGAAATCACTGTTTCCACATAGGTGAGAAAAGCGTTATTGGTCCGGTATACCTGCGAAGCGACAAAGCAATAAGGCAACAATGGCAGGTAATGCTGCTCaaaaa      <  1:2497835/148‑1 (MQ=255)
                                                                                                                                              tctcAACAAACTCTTCCAGCCCTGTTTCGCGGAAATTCAATGAAGAAATCACTGTTTCCACATAGGTGAGAAAAGCGTTATTGGTCCGGTATACCTGCGAAGCGACAAAGCAATAAGGCACCAATGGCAGGTAATGCTGCTCaaaaaa     >  2:2839149/1‑148 (MQ=255)
                                                                                                                                               ctcAACAAACTCTTCCAGCCCTGTTTCGCGGAAATTCAATGAAGAAATCACTGTTTCCACATAGGTGAGAAAAGCGTTATTGGTCCGGTATACCTGCGAAGCGACAAAGCAATAAGGCAACAATGGCAGGTAATGCTGCTCAAAAAAg    >  2:240687/1‑148 (MQ=255)
                                                                                                                                                 caacaaACTCTTCCAGCCCTGTTTCGCGGAAATTCAATGAAGAAATCACTGTTTCCACATAGGTGAGAAAAGCGTTATTGGTCCGGTATACCTGCGAAGCGACAAAGCAATAAGGCAACAATGGCAGGTAATGCTGCTCAAAAAAGCg  <  1:316170/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                   
ACCATATTCTCCAGCGCCGATTCACTAAACTGCTCTTTATCATGCATCTTTTCTACCGTACTTTTCAGCCACTGCGGTACATCATCAATCACCTGTTCTTCGCGGTAATGACGTAAACGGTTAATGACATAAAAAGTAACCATCTCAACAAACTCTTCCAGCCCTGTTTCGCGGAAATTCAATGAAGAAATCACTGTTTCCACATAGGTGAGAAAAGCGTTATTGGTCCGGTATACCTGCGAAGCGACAAAGCAATAAGGCAACAATGGCAGGTAATGCTGCTCAAAAAAGCG  >  CP009273/1813118‑1813410

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A