Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F309 I0 R1 1697 197.2 6627756 95.7% 6342762 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,141,613 G→T 42.8% V153V (GTG→GTT)  yegD → Hsp70 chaperone family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,141,6130GT42.8% 171.6 / 173.2 165V153V (GTG→GTT) yegDHsp70 chaperone family protein
Reads supporting (aligned to +/- strand):  ref base G (47/47);  new base T (38/33);  total (85/80)
Fisher's exact test for biased strand distribution p-value = 7.53e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 166 total aligned reads displayed.

TTTGTGAAATCACCAAAATCGTTCCTCGGTGCCAGCGGCTTAAAACCGCAGCAGGTAGCGCTGTTTGAGGATCTGGTCTGCGCAATGATGTTGCACATTCGCCAGCAGGCGCAGGCACAGCTGCCAGAAGCGATTACTCAGGCGGTGATTGGTCGTCCGATCAACTTCCAGGGGCTGGGCGGTGATGAAGCAAACACCCAGGCGCAAGGGATTCTGGAACGCGCGGCGAAGCGTGCCGGATTCAGGGACGTGGTATTCCAGTACGAGCCGGTCGCGGCTGGGCTGGATTACGAA  >  CP009273/2141467‑2141760
                                                                                                                                                  |                                                                                                                                                   
tttGTGAAATCACCAAAATCGTTCCTCGGTGCCAGCGGCTTAAAACCGCAGCAGGTAGCGCTGTTTGAGGATCTGGTCTGCGCAATGATGTTGCACATTCGCCAGCAGGCGCAGGCACAGCTGCCAGAAGCGATTACTCAGGCGGTGa                                                                                                                                                    >  2:580495/1‑148 (MQ=255)
 ttGTGAAATCACCAAAATCGTTCCTCGGTGCCAGCGGCTTAAAACCGCAGCAGGTAGCGCTGTTTGAGGATCTGGTCTGCGCAATGATGTTGCACATTCGCCAGCAGGCGCAGGCACAGCTGCCAGAAGCGATTACTCAGGCGGTGAt                                                                                                                                                   >  2:1527704/1‑148 (MQ=255)
   gtgAAATCACCAAAATCGTTCCTCGGTGCCAGCGGCTTAAAACCGCAGCAGGTAGCGCTGTTTGAGGATCTGGTCTGCGCAATGATGTTGCACATTCGCCAGCAGGCGCAGGCACAGCTGCCAGAAGCGATTACTCAGGCGGTTATTg                                                                                                                                                 <  2:1998679/148‑1 (MQ=255)
    tgAAATCACCAAAATCGTTCCTCGGTGCCAGCGGCTTAAAACCGCAGCAGGTAGCGCTGTTTGAGGATCTGGTCTGCGCAATGATGTTGCACATTCGCCAGCAGGCGCAGGCACAGCTGCCAGAAGCGATTACTCAGGCGGTTATTgg                                                                                                                                                >  2:2021389/1‑148 (MQ=255)
    tgAAATCACCAAAATCGTTCCTCGGTGCCAGCGGCTTAAAACCGCAGCAGGTAGCGCTGTTTGAGGATCTGGTCTGCGCAATGATGTTGCACATTCGCCAGCAGGCGCAGGCACAGCTGCCAGAAGCGATTACTCAGGCGGTGATTgg                                                                                                                                                <  1:1820486/148‑1 (MQ=255)
           aCCAAAATCGTTCCTCGGTGCCAGCGGCTTAAAACCGCAGCAGGTAGCGCTGTTTGAGGATCTGGTCTGCGCAATGATGTTGCACATTCGCCAGCAGGCGCAGGCACAGCTGCCAGAAGCGATTACTCAGGCGGTTATTGGTCGTCCg                                                                                                                                         >  2:495647/1‑148 (MQ=255)
            ccAAAATCGTTCCTCGGTGCCAGCGGCTTAAAACCGCAGCAGGTAGCGCTGTTTGAGGATCTGGTCTGCGCAATGATGTTGCACATTCGCCAGCAGGCGCAGGCACAGCTGCCAGAAGCGATTACTCAGGCGGTGATTGGTCGTCCGa                                                                                                                                        >  1:1070111/1‑148 (MQ=255)
            ccAAAATCGTTCCTCGGTGCCAGCGGCTTAAAACCGCAGCAGGTAGAGCTGTTTGAGGATCAGGTCTGCGCAATGATGTTGCACATTCGCCAGCAGGCGCAGGCACAGCTGCCAGAAGCGATTACTCAGGCGGTGATTGGTCGTCCGa                                                                                                                                        >  1:3249658/1‑148 (MQ=255)
              aaaaTCGGTCCTCGGTGCCAGCGGCTTAAAACCGCAGCAGGTAGCGCTGTTTGAGGATCTGGTCTGCGCAATGATGTTGCACATTCGCCAGCAGGCGCAGGCACAGCTGCCAGAAGCGATTACTCAGGCGGTTATTGGTCGTCCGATc                                                                                                                                      <  2:532390/148‑1 (MQ=255)
               aaaTCGTTCCTCGGTGCCAGCGGCTTAAAACCGCAGCAGGTAGCGCTGTTTGAGGATCTGGTCTGCGCAATGATGTTGCACATTCGCCAGCAGGCGCAGGCACAGCTGCCCGAAGCGATTACTCAGGCGGTTATTGGTCGTCCGATCa                                                                                                                                     >  2:2153312/1‑148 (MQ=255)
               aaaTCGTTCCTCGGTGCCAGCGGCTTAAAACCGCAGCAGGTAGCGCTGTTTGAGGATCTGGTCTGCGCAATGATGTTGCACATTCGCCAGCAGGCGCAGGCACAGCTGCCAGAAGCGATTACTCAGGCGGTTATTGGTCGTCCGATCa                                                                                                                                     <  2:1265576/148‑1 (MQ=255)
                aaTCGTTCCTCGGTGCCAGCGGCTTAAAACCGCAGCAGGTAGCGCTGTTTGAGGATCTGGTCTGCGCAATGATGTTGCACATTCGCCAGCAGGCGCAGGCACAGCTGCCAGAAGCGATTACTCAGGCGGTTATTGGTCGTCCGATCaa                                                                                                                                    >  2:660870/1‑148 (MQ=255)
                aaTCGTTCCTCGGTGCCAGCGGCTTAAAACCGCAGCAGGTAGCGCTGTTTGAGGATCTGGTCTGCGCAATGATGTTGCACATTCGCCAGCAGGCGCAGGCACAGCTGCCAGAAGCGATTACTCAGGCGGTTATTGGTCGTCCGATCaa                                                                                                                                    >  2:660880/1‑148 (MQ=255)
                 aTCGTTCCTCGGTGCCAGCGGCTTAAAACCGCAGCAGGTAGCGCTGTTTGAGGATCTGGTCTGCGCAATGATGTTGCACATTCGCCAGCAGGCGCAGGCACAGCTGCCAGAAGCGATTACTCAGGCGGTGATTGGTCGTCCGATCAAc                                                                                                                                   >  2:454493/1‑148 (MQ=255)
                   cGTTCCTCGGTGCCAGCGGCTTAAAACCGCAGCAGGTAGCGCTGTTTGAGGATCTGGTCTGTGCAATGATGTTGCACATTCGCCAGCAGGCGCAGGCACAGCTGCCAGAAGCGATTACTCAGGCGGTTATTGGTCGTCCGATCAACtt                                                                                                                                 <  1:1670273/148‑1 (MQ=255)
                    gTTCCTCGGTGCCAGCGGCTTAAAACCGCAGCAGGTAGCGCTGTTTGAGGATCTGGTCTGCGCAATGATGTTGCACATTCGCCAGCAGGCGCAGGCACAGCTGCCAGAAGCGATTACTCAGGCGGTTATTGGTCGTCCGATCAACTTc                                                                                                                                <  2:2644474/148‑1 (MQ=255)
                     ttCCTCGGTGCCAGCGGCTTAAAACCGCAGCAGGTAGCGCTGTTTGAGGATCTGGTCTGCGCAATGATGTTGCACATTCGCCAGCAGGCGCAGGCACAGCTGCCAGAAGCGATTACTCAGGCGGTTATTGGTCGTCCGATCAACTTcc                                                                                                                               >  2:653397/1‑148 (MQ=255)
                      tCCTCGGTGCCAGCGGCTTAAAACCGCAGCAGGTAGCGCTGTTTGAGGATCTGGTCTGCGCAATGATGTTGCACATTCGCCAGCAGGCGCAGGCACAGCTGCCAGAAGCGATTACTCAGGCGGTTATTGGTCGTCCGATCAACTTCCa                                                                                                                              <  1:23651/148‑1 (MQ=255)
                       ccTCGGTGCCAGCGGCTTAAAACCGCAGCAGGTAGCGCTGTTTGAGGATCTGGTCTGCGCAATGATGTTGCACATTCGCCAGCAGGCGCAGGCACAGCTGCCAGAAGCGATTACTCAGGCGGTTATTGGTCGTCCGATCAACTTCCAg                                                                                                                             >  2:880663/1‑148 (MQ=255)
                       ccTCGGTGCCAGCGGCTTAAAACCGCAGCAGGTAGCGCTGTTTGAGGATCTGGTCTGCGCAATGATGTTGCACATTCGCCAGCAGGCGCAGGCACAGCTGCCAGAAGCGATTACTCAGGCGGTTATTGGTCGTCCGATCAACTTCCAg                                                                                                                             >  2:2923692/1‑148 (MQ=255)
                          cGGTGCCAGCGGCTTAAAACCGCAGCAGGTAGCGCTGTTTGAGGATCTGGTCTGCGCAATGATGTTGCACATTCGCCAGCAGGCGCAGGCACAGCTGCCAGAAGCGATTACTCAGGCGGTTATTGGTCGTCCGATCAACTTCCAgggg                                                                                                                          >  2:2157212/1‑148 (MQ=255)
                          cGGTGCCAGCGGCTTAAAACCGCAGCAGGTAGCGCTGTTTGAGGATCTGGTCTGCGCAATGATGTTGCACATTCGCCAGCAGGCGCAGGCACAGCTGCCAGAAGCGATTACTCAGGCGGTGATTGGTCGTCCGATCAACTTCCAgggg                                                                                                                          <  1:1499393/148‑1 (MQ=255)
                            gTGCCAGCGGCTTAAAACCGCAGCAGGTAGCGCTGTTTGAGGATCTGGTCTGCGCAATGATGTTGCACATTCGCCAGCAGGCGCAGGCACAGCTGCCAGAAGCGATTACTCAGGCGGTGATTGGTCGTCCGATCAACTTCCAGGGGCt                                                                                                                        >  2:836241/1‑148 (MQ=255)
                               ccAGCGGCTTAAAACCGCAGCAGGTAGCGCTGTTTGAGGATCTGGTCTGCGCAATGATGTTGCACATTCGCCAGCAGGCGCAGGCACAGCTGCCAGAAGCGATTACTCAGGCGGTGATTGGTCGTCCGATCAACTTCCAGGGGCTggg                                                                                                                     >  1:405424/1‑148 (MQ=255)
                               ccAGCGGCTTAAAACCGCAGCAGGTAGCGCTGTTTGAGGATCTGGTCTGCGCAATGATGTTGCACATTCGCCAGCAGGCGCAGGCACAGCTGCCAGAAGCGATTACTCAGGCGGTGATTGGTCGTCCGATCAACTTCCAGGGGCTggg                                                                                                                     >  1:405447/1‑148 (MQ=255)
                                  gCGGCTTAAAACCGCAGCAGGTAGCGCTGTTTGAGGATCTGGTCTGCGCAATGATGTTGCACATTCGCCAGCAGGCGCAGGCACAGCTGCCAGAAGCGATTACTCAGGCGGTGATTGGTCGTCCGATCAACTTCCAGGGGCTGGGCgg                                                                                                                  <  1:2403012/148‑1 (MQ=255)
                                  gCGGCTTAAAACCGCAGCAGGTAGCGCTGTTTGAGGATCTGGTCTGCGCAATGATGTTGCACATTCGCCAGCAGGCGCAGGCACAGCTGCCAGAAGCGATTACTCAGGCGGTGATTGGTCGTCCGATCAACTTCCAGGGGCTGGGCgg                                                                                                                  >  1:2072314/1‑148 (MQ=255)
                                  gCGGCTTAAAACCGCAGCAGGTAGCGCTGTTTGAGGATCTGGTCTGCGCAATGATGTTGCACATTCGCCAGCAGGCGCAGGCACAGCTGCCAGAAGCGATTACTCAGGCGGTGATTGGTCGTCCGATCAACTTCCAGGGGCTGGGCgg                                                                                                                  >  2:2321833/1‑148 (MQ=255)
                                     gCTTAAAACCGCAGCAGGTAGCGCTGTTTGAGGATCTGGTCTGCGCAATGATGTTGCACATTCGCCAGCAGGCGCAGGCACAGCTGCCAGAAGCGATTACTCAGGCGGTTATTGGTCGTCCGATCAACTTCCAGGGGCTGGGCGGtga                                                                                                               >  2:1218338/1‑148 (MQ=255)
                                     gCTTAAAACCGCAGCAGGTAGCGCTGTTTGAGGATCTGGTCTGCGCAATGATGTTGCACATTCGCCAGCAGGCGCAGGCACAGCTGCCAGAAGCGATTACTCAGGCGGTGATTGGTCGTCCGATCAACTTCCAGGGGCTGGGCGGtga                                                                                                               <  1:2183100/148‑1 (MQ=255)
                                         aaaaCCGCAGCAGGTAGCGCTGTTTGAGGATCTGGTCTGCGCAATGATGTTGCACATTCGCCAGCAGGCGCAGGCACAGCTGCCAGAAGCGATTACTCAGGCGGTTATTGGTCGTCCGATCAACTTCCAGGGGCTGGGCGGTGATGaa                                                                                                           >  2:1801851/1‑148 (MQ=255)
                                         aaaaCCGCAGCAGGTAGCGCTGTTTGAGGATCTGGTCTGCGCAATGATGTTGCACATTCGCCAGCAGGCGCAGGCACAGCTGCCAGAAGCGATTACTCAGGCGGTTATTGGTCGTCCGATCAACTTCCAGGGGCTGGGCGGTGATGaa                                                                                                           <  1:610467/148‑1 (MQ=255)
                                           aaCCGCAGCAGGTAGCGCTGTTTGAGGATCTGGTCTGCGCAATGATGTTGCACATTCGCCAGCAGGCGCAGGCACAGCTGCCAGAAGCGATTACTCAGGCGGTGATTGGTCGTCCGATCAACTTCCAGGGGCTGGGCGGTGATGAAGc                                                                                                         >  1:2945462/1‑148 (MQ=255)
                                            aCCGCAGCAGGTAGCGCTGTTTGAGGATCTGGTCTGCGCAATGATGTTGCACATTCGCCAGCAGGCGCAGGCACAGCTGCCAGAAGCGATTACTCAGGCGGTGATTGGTCGTCCGATCAACTTCCAGGGGCTGGGCGGTGATGAAGCa                                                                                                        >  2:2829972/1‑148 (MQ=255)
                                               gcagcaGGTAGCGCTGTTTGAGGATCTGGTCTGCGCAATGATGTTGCACATTCGCCAGCAGGCGCAGGCACAGCTGCCAGAAGCGATTACTCAGGCGGTTATTGGTCGTCCGATCAACTTCCAGGGGCTGGGCGGTGATGAAGCAAac                                                                                                     >  1:3028728/1‑148 (MQ=255)
                                               gcagcaGGTAGCGCTGTTTGAGGATCTGGTCTGCGCAATGATGTTGCACATTCGCCAGCAGGCGCAGGCACAGCTGCCAGAAGCGATTACTCAGGCGGTTATTGGTCGTCCGATCAACTTCCAGGGGCTGGGCGGTGATGAAGCAAac                                                                                                     >  1:1379830/1‑148 (MQ=255)
                                                   cagGTAGCGCTGTTTGAGGATCTGGTCTGCGCAATGATGTTGCACATTCGCCAGCAGGCGCAGGCACAGCTGCCAGAAGCGATTACTCAGGCGGTTATTGGTCGTCCGATCAACTTCCAGGGGCTGGGCGGTGATGAAGCAAACAccc                                                                                                 >  1:1006110/1‑148 (MQ=255)
                                                    agGTAGCGCTGTTTGAGGATCTGGTCTGCGCAATGATGTTGCACATTCGCCAGCAGGCGCAGGCACAGCTGCCAGAAGCGATTACTCAGGCGGTGATTGGTCGTCCGATCAACTTCCAGGGGCTGGGCGGTGATGAAGCAAACACCCa                                                                                                <  1:2440995/148‑1 (MQ=255)
                                                      gTAGCGCTGTTTGAGGATCTGGTCTGCGCAATGATGTTGCACATTCGCCAGCAGGCGCAGGCACAGCTGCCAGAAGCGATTACTCAGGCGGTTATTGGTCGTCCGATCAACTTCCAGGGGCTGGGCGGTGATGAAGCAAACACCCAgg                                                                                              >  1:995664/1‑148 (MQ=255)
                                                        aGCGCTGTTTGAGGATCTGGTCTGCGCAATGATGTTGCACATTCGCCAGCAGGCGCAGGCACAGCTGCCAGAAGCGATTACTCAGGCGGTTATTGGTCGTCCGATCAACTTCCAGGGGCTGGGCGGTGATGAAGCAAACACCCAGgcg                                                                                            <  2:2766357/148‑1 (MQ=255)
                                                           gcTGTTTGAGGATCTGGTCTGCGCAATGATGTTGCACATTCGCCAGCAGGCGCAGGCACAGCTGCCAGAAGCGATTACTCAGGCGGTTATTGGTCGTCCGATCAACTTCCAGGGGCTGGGCGGTGATGAAGCAAACACCCAGGCGCaa                                                                                         <  1:1785056/148‑1 (MQ=255)
                                                            cTGTTTGAGGATCTGGTCTGCGCAATGATGTTGCACATTCGCCAGCAGGCGCAGGCACAGCTGCCAGAAGCGATTACTCAGGCGGTTATTGGTCGTCCGATCAACTTCCAGGGGCTGGGCGGTGATGAAGCAAACACCCAGGCGCAAg                                                                                        >  1:866140/1‑148 (MQ=255)
                                                            cTGTTTGAGGATCTGGTCTGCGCAATGATGTTGCACATTCGCCAGCAGGCGCAGGCACAGCTGCCAGAAGCGATTACTCAGGCGGTTATTGGTCGTCCGATCAACTTCCAGGGGCTGGGCGGTGATGAAGCAAACACCCAGGCGCAAg                                                                                        >  1:866139/1‑148 (MQ=255)
                                                            cTGTTTGAGGATCTGGTCTGCGCAATGATGTTGCACATTCGCCAGCAGGCGCAGGCACAGCTGCCAGAAGCGATTACTCAGGCGGTTATTGGTCGTCCGATCAACTTCCAGGGGCTGGGCGGTGATGAAGCAAACACCCAGGCGCAAg                                                                                        >  2:724897/1‑148 (MQ=255)
                                                              gTTTGAGGATCTGGTCTGCGCAATGATGTTGCACATTCGCCAGCAGGCGCAGGCACAGCTGCCAGAAGCGATTACTCAGGCGGTGATTGGTCGTCCGATCAACTTCCAGGGGCTGGGCGGTGATGAAGCAAACACCCAGGCGCAAggg                                                                                      >  2:2425522/1‑148 (MQ=255)
                                                              gTTTGAGGATCTGGTCTGCGCAATGATGTTGCACATTCGCCAGCAGGCGCAGGCACAGCTGCCAGAAGCGATTACTCAGGCGGTGATTGGTCGTCCGATCAACTTCCAGGGGCTGGGCGGTGATGAAGCAAACACCCAGGCGCAAggg                                                                                      >  2:1208927/1‑148 (MQ=255)
                                                               tttGAGGATCTGGTCTGCGCAATGATGTTGCACATTCGCCAGCAGGCGCAGGCACAGCTGCCAGAAGCGATTACTCAGGCGGTGATTGGTCGTCCGATCAACTTCCAGGGGCTGGGCGGTGATGAAGCAAACACCCAGGCGCAAGGGa                                                                                     >  2:222114/1‑148 (MQ=255)
                                                                ttGAGGATCTGGTCTGCGCAATGATGTTGCACATTCGCCAGCAGGCGCAGGCACAGCTGCCAGAAGCGATTACTCAGGCGGTGATTGGTCGTCCGATCAACTTCCAGGGGCTGGGCGGTGATGAAGCAAACACCCAGGCGCAAGGGAt                                                                                    <  1:853086/148‑1 (MQ=255)
                                                                  gAGGATCTGGTCTGCGCAATGATGTTGCACATTCGCCAGCAGGCGCAGGCACAGCTGCCAGAAGCGATTACTCAGGCGGTTATTGGTCGTCCGATCAACTTCCAGGGGCTGGGCGGTGATGAAGCAAACACCCAGGCGCAAGGGATTc                                                                                  <  1:2832339/148‑1 (MQ=255)
                                                                  gAGGATCTGGTCTGCGCAATGATGTTGCACATTCGCCAGCAGGCGCAGGCACAGCTGCCAGAAGCGATTACTCAGGCGGTGATTGGTCGTCCGATCAACTTCCAGGGGCTGGGCGGTGATGAAGCAAACACCCAGGCGCAAGGGATTc                                                                                  <  2:3263467/148‑1 (MQ=255)
                                                                      aTCTGGTCTGCGCAATGATGTTGCACATTCGCCAGCAGGCGCAGGCACAGCTGCCAGAAGCGATTACTCAGGCGGTGATTGGTCGTCCGATCAACTTCCAGGGGCTGGGCGGTGATGAAGCAAACACCCAGGCGCAAGGGATTCTGGa                                                                              >  1:2805559/1‑148 (MQ=255)
                                                                      aTCTGGTCTGCGCAATGATGTTGCACATTCGCCAGCAGGCGCAGGCACAGCTGCCAGAAGCGATTACTCAGGCGGTGATTGGTCGTCCGATCAACTTCCAGGGGCTGGGCGGTGATGAAGCAAACACCCAGGCGCAAGGGATTCTGGa                                                                              <  1:1149083/148‑1 (MQ=255)
                                                                           gTCTGCGCAATGATGTTGCACATTCGCCAGCAGGCGCAGGCACAGCTGCCAGAAGCGATTACTCAGGCGGTTATTGGTCGTCCGATCAACTTCCAGGGGCTGGGCGGTGATGAAGCAAACACCCAGGCGCAAGGGATTCTGGAAcgcg                                                                         >  2:2423307/1‑148 (MQ=255)
                                                                           gTCTGCGCAATGATGTTGCACATTCGCCAGCAGGCGCAGGCACAGCTGCCAGAAGCGATTACTCAGGCGGTTATTGGTCGTCCGATCAACTTCCAGGGGCTGGGCGGTGATGAAGCAAACACCCAGGCGCAAGGGATTCTGGAAcgcg                                                                         >  2:1718182/1‑148 (MQ=255)
                                                                           gTCTGCGCAATGATGTTGCACATTCGCCAGCAGGCGCAGGCACAGCTGCCAGAAGCGATTACTCAGGCGGTGATTGGTCGTCCGATCAACTTCCAGGGGCTGGGCGGTGATGAAGCAAACACCCAGGCGCAAGGGATTCTGGAAcgcg                                                                         >  1:1217129/1‑148 (MQ=255)
                                                                           gTCTGCGCAATGATGTTGCACATTCGCCAGCAGGCGCAGGCACAGCTGCCAGAAGCGATTACTCAGGCGGTGATTGGTCGTCCGATCAACTTCCAGGGGCTGGGCGGTGATGAAGCAAACACCCAGGCGCAAGGGATTCTGGAAcgcg                                                                         <  2:1842721/148‑1 (MQ=255)
                                                                            tCTGCGCACTGATGTTGCACATTCGCCAGCAGGCGCAGGCACAGCTGCCAGAAGCGATTACTCAGGCGGTGATTGGTCGTCCGATCAACTTCCAGGGGCTGGGCGGTGATGAAGCAAACACCCAGGCGCAAGGGATTCTGGAAcgcgc                                                                        <  2:1962866/148‑1 (MQ=255)
                                                                             cTGCGCAATGATGTTGCACATTCGCCAGCAGGCGCAGGCACAGCTGCCAGAAGCGATTACTCAGGCGGTGATTGGTCGTCCGATCAACTTCCAGGGGCTGGGCGGTGATGAAGCAAACACCCAGGCGCAAGGGATTCTGGAAcgcgcg                                                                       >  1:753421/1‑148 (MQ=255)
                                                                             cTGCGCAATGATGTTGCACATTCGCCAGCAGGCGCAGGCACAGCTGCCAGAAGCGATTACTCAGGCGGTGATTGGTCGTCCGATCAACTTCCAGGGGCTGGGCGGTGATGAAGCAAACACCCAGGCGCAAGGGATTCTGGAAcgcgcg                                                                       >  1:1075126/1‑148 (MQ=255)
                                                                              tGCGCAATGATGTTGCACATTCGCCAGCAGGCGCAGGCACAGCTGCCAGAAGCGATTACTCAGGCGGTGATTGGTCGTCCGATCAACTTCCAGGGGCTGGGCGGTGATGAAGCAAACACCCAGGCGCAAGGGATTCTGGAACGCgcgg                                                                      <  1:2901531/148‑1 (MQ=255)
                                                                                cgcAATGATGTTGCACATTCGCCAGCAGGCGCAGGCACAGCTGCCAGAAGCGATTACTCAGGCGGTGATTGGTCGTCCGATCAACTTCCAGGGGCTGGGCGGTGATGAAGCAAACACCCAGGCGCAAGGGATTCTGGAACGCgcggcg                                                                    >  2:1589720/1‑148 (MQ=255)
                                                                                    atgatgTTGCACATTCGCCAGCAGGCGCAGGCACAGCTGCCAGAAGCGATTACTCAGGCGGTGATTGGTCGTCCGATCAACTTCCAGGGGCTGGGCGGTGATGAAGCAAACACCCAGGCGCAAGGGATTCTGGAACGCGCGGCGAAGc                                                                <  2:2573137/148‑1 (MQ=255)
                                                                                    atgatgTTGCACATTCGCCAGCAGGCGCAGGCACAGCTGCCAGAAGCGATTACTCAGGCGGTGATTGGTCGTCCGATCAACTTCCAGGGGCTGGGCGGTGATGAAGCAAACACCCAGGCGCAAGGGATTCTGGAACGCGCGGCGAAGc                                                                <  2:377037/148‑1 (MQ=255)
                                                                                     tgatgTTGCACATTCGCCAGCAGGCGCAGGCACAGCTGCCAGAAGCGATTACTCAGGCGGTGATTGGTCGTCCGATCAACTTCCAGGGGCTGGGCGGTGATGAAGCAAACACCCAGGCGCAAGGGATTCTGGAACGCGCGGCGAAGCg                                                               >  1:2303789/1‑148 (MQ=255)
                                                                                      gatgTTGCACATTCGCCAGCAGGCGCAGGCACAGCTGCCAGAAGCGATTACTCAGGCGGTGATTGGTCGTCCGATCAACTTCCAGGGGCTGGGCGGTGATGAAGCAAACACCCAGGCGCAAGGGATTCTGGAACGCGCGGCGAAGCGt                                                              >  2:164754/1‑148 (MQ=255)
                                                                                         gTTGCACATTCGCCAGCAGGCGCAGGCACAGCTGCCAGAAGCGATTACTCAGGCGGTGATTGGTCGTCCGATCAACTTCCAGGGGCTGGGCGGTGATGAAGCAAACACCCAGGCGCAAGGGATTCTGGAACGCGCGGCGAAGCGTGcc                                                           >  2:2398076/1‑148 (MQ=255)
                                                                                           tGCACATTCGCCAGCAGGCGCAGGCACAGCTGCCAGAAGCGATTACTCAGGCGGTGATTGGTCGTCCGATCAACTTCCAGGGGCTGGGCGGTGATGAAGCAAACACCCAGGCGCAAGGGATTCTGGAACGCGCGGCGAAGCGTGCCgg                                                         >  1:741160/1‑148 (MQ=255)
                                                                                             cacaTTCGCCAGCAGGCGCAGGCACAGCTGCCAGAAGCGATTACTCAGGCGGTTATTGGTCGTCCGATCAACTTCCAGGGGCTGGGCGGTGATGAAGCAAACACCCAGGCGCAAGGGATTCTGGAACGCGCGGCGAAGCGTGCCGGAt                                                       >  2:631336/1‑148 (MQ=255)
                                                                                             cacaTTCGCCAGCAGGCGCAGGCACAGCTGCCAGAAGCGATTACTCAGGCGGTTATTGGTCGTCCGATCAACTTCCAGGGGCTGGGCGGTGATGAAGCAAACACCCAGGCGCAAGGGATTCTGGAACGCGCGGCGAAGCGTGCCGGAt                                                       <  1:132282/148‑1 (MQ=255)
                                                                                             cacaTTCGCCAGCAGGCGCAGGCACAGCTGCCAGAAGCGATTACTCAGGCGGTTATTGGTCGTCCGATCAACTTCCAGGGGCTGGGCGGTGATGAAGCAAACACCCAGGCGCAAGGGATTCTGGAACGCGCGGCGAAGCGTGCCGGAt                                                       <  1:132176/148‑1 (MQ=255)
                                                                                               caTTCGCCAGCAGGCGCAGGCACAGCTGCCAGAAGCGATTACTCAGGCGGTTATTGGTCGTCCGATCAACTTCCAGGGGCTGGGCGGTGATGAAGCAAACACCCAGGCGCAAGGGATTCTGGAACGCGCGGCGAAGCGTGCCGGATTc                                                     >  1:268115/1‑148 (MQ=255)
                                                                                               caTTCGCCAGCAGGCGCAGGCACAGCTGCCAGAAGCGATTACTCAGGCGGTGATTGGTCGTCCGATCAACTTCCAGGGGCTGGGCGGTGATGAAGCAAACACCCAGGCGCAAGGGATTCTGGAACGCGCGGCGAAGCGTGCCGGATTc                                                     <  1:596870/148‑1 (MQ=255)
                                                                                                 ttCGCCAGCAGGCGCAGGCACAGCTGCCAGAAGCGATTACTCAGGCGGTTATTGGTCGTCCGATCAACTTCCAGGGGCTGGGCGGTGATGAAGCAAACACCCAGGCGCAAGGGATTCTGGAACGCGCGGCGAAGCGTGCCGGATTCAg                                                   >  2:2708463/1‑148 (MQ=255)
                                                                                                 ttCGCCAGCAGGCGCAGGCACAGCTGCCAGAAGCGATTACTCAGGCGGTGATTGGTCGTCCGATCAACTTCCAGGGGCTGGGCGGTGATGAAGCAAACACCCAGGCGCAAGGGATTATGGAACGCGCGGCGAAGCGTGCCGGATTCAg                                                   <  1:50801/148‑1 (MQ=255)
                                                                                                  tCGCCAGCAGGCGCAGGCACAGCTGCCAGAAGCGATTACTCAGGCGGTGATTGGTCGTCCGATCAACTTCCAGGGGCTGGGCGGTGATGAAGCAAACACCCAGGCGCAAGGGATTCTGGAACGCGCGGCGAAGCGTGCCGGATTCAgg                                                  <  2:478023/148‑1 (MQ=255)
                                                                                                      cagcagGCGCAGGCACAGCTGCCAGAAGCGATTACTCAGGCGGTTATTGGTCGTCCGATCAACTTCCAGGGGCTGGGCGGTGATGAAGCAAACACCCAGGCGCAAGGGATTCTGGAACGCGCGGCGAAGCGTGCCGGATTCAGGGACg                                              >  1:1572685/1‑148 (MQ=255)
                                                                                                       agcagGCGCAGGCACAGCTGCCAGAAGCGATTACTCAGGCGGTGATTGGTCGTCCGATCAACTTCCAGGGGCTGGGCGGTGATGAAGCAAACACCCAGGCGCAAGGGATTCTGGAACGCGCGGCGAAGCGTGCCGGATTCAGGGACGt                                             <  2:617000/148‑1 (MQ=255)
                                                                                                         caggcgcaggcACAGCTGCCAGAAGCGATTACTCAGGCGGTGATTGGTCGTCCGATCAACTTCCAGGGGCTGGGCGGTGATGAAGCAAACACCCAGGCGCAAGGGATTCTGGAACGCGCGGCGAAGCGTGCCGGATTCAGGGACGTgg                                           >  2:3099915/1‑148 (MQ=255)
                                                                                                          aggcgcaggcACAGCTGCCAGAAGCGATTACTCAGGCGGTGATTGGTCGTCCGATCAACTTCCAGGGGCTGGGCGGTGATGAAGCAAACACCCAGGCGCAAGGGATTCTGGAACGCGCGGCGAAGCGTGCCGGATTCAGGGACGTGGt                                          <  2:2791463/148‑1 (MQ=255)
                                                                                                            gcgcaggcACAGCTGCCAGAAGCGATTACTCAGGCGGTGATTGGTCGTCCGATCAACTTCCAGGGGCTGGGCGGTGATGAAGCAAACACCCAGGCGCAAGGGATTCTGGAACGCGCGGCGAAGCGTGCCGGATTCAGGGACGTGGTAt                                        <  2:509816/148‑1 (MQ=255)
                                                                                                             cgcaggcACAGCTGCCAGAAGCGATTACTCAGGCGGTTATTGGTCGTCCGATCAACTTCCAGGGGCTGGGCGGTGATGAAGCAAACACCCAGGCGCAAGGGATTCTGGAACGCGCGGCGAAGCGTGCCGGATTCAGGGACGTGGTAtt                                       <  1:3277102/148‑1 (MQ=255)
                                                                                                              gcaggcACAGCTGCCAGAAGCGATTACTCAGGCGGTTATTGGTCGTCCGATCAACTTCCAGGGGCTGGGCGGTGATGAAGCAAACACCCAGGCGCAAGGGATTCTGGAACGCGCGGCGAAGCGTGCCGGATTCAGGGACGTGGTATTc                                      <  2:866139/148‑1 (MQ=255)
                                                                                                                aggcACAGCTGCCAGAAGCGATTACTCAGGCGGTGATTGGTCGTCCGATCAACTTCCAGGGGCTGGGCGGTGATGAAGCAAACACCCAGGCGCAAGGGATTCTGGAACGCGCGGCGAAGCGTGCCGGATTCAGGGACGTGGTATTCCa                                    <  2:2299880/148‑1 (MQ=255)
                                                                                                                  gcACAGCTGCCAGAAGCGATTACTCAGGCGGTTATTGGTCGTCCGATCAACTTCCAGGGGCTGGGCGGTGATGAAGCAAACACCCAGGCGCAAGGGATTCTGGAACGCGCGGCGAAGCGTGCCGGATTCAGGGACGTGGTATTCCAGt                                  >  2:1484858/1‑148 (MQ=255)
                                                                                                                     caGCTGCCAGAAGCGATTACTCAGGCGGTTATTGGTCGTCCGATCAACTTCCAGGGGCTGGGCGGTGATGAAGCAAACACCCAGGCGCAAGGGATTCTGGAACGCGCGGCGAAGCGTGCCGGATTCAGGGACGTGGTATTCCAGTACg                               <  2:2706790/148‑1 (MQ=255)
                                                                                                                     caGCTGCCAGAAGCGATTACTCAGGCGGTTATTGGTCGTCCGATCAACTTCCAGGGGCTGGGCGGTGATGAAGCAAACACCCAGGCGCAAGGGATTCTGGAACGCGCGGCGAAGCGTGCCGGATTCAGGGACGTGGTATTCCAGTACg                               <  2:2702691/148‑1 (MQ=255)
                                                                                                                     caGCTGCCAGAAGCGATTACTCAGGCGGTTATTGGTCGTCCGATCAACTTCCAGGGGCTGGGCGGTGATGAAGCAAACACCCAGGCGCAAGGGATTCTGGAACGCGCGGCGAAGCGTGCCGGATTCAGGGACGTGGTATTCCAGTACg                               <  2:1049830/148‑1 (MQ=255)
                                                                                                                     caGCTGCCAGAAGCGATTACTCAGGCGGTTATTGGTCGTCCGATCAACTTCCAGGGGCTGGGCGGTGATGAAGCAAACACCCAGGCGCAAGGGATTCTGGAACGCGCGGCGAAGCGTGCCGGATTCAGGGACGTGGTATTCCAGTACg                               <  2:1049792/148‑1 (MQ=255)
                                                                                                                            cAGAAGCGATTACTCAGGCGGTGATTGGTCGTCCGATCAACTTCCAGGGGCTGGGCGGTGATGAAGCAAACACCCAGGCGCAAGGGATTCTGGAACGCGCGGCGAAGCGTGCCGGATTCAGGGACGTGGTATTCCAGTACGAGCCGGt                        <  1:1669209/148‑1 (MQ=255)
                                                                                                                            cAGAAGCGATTACTCAGGCGGTGATTGGTCGTCCGATCAACTTCCAGGGGCTGGGCGGTGATGAAGCAAACACCCAGGCGCAAGGGATTCTGGAACGCGCGGCGAAGCGTGCCGGATTCAGGGACGTGGTATTCCAGTACGAGCCGGt                        <  1:1051850/148‑1 (MQ=255)
                                                                                                                             aGAAGCGATTACTCAGGCGGTGATTGGTCGTCCGATCAACTTCCAGGGGCTGGGCGGTGATGAAGCAAACACCCAGGCGCAAGGGATTCTGGAACGCGCGGCGAAGCGTGCCGGATTCAGGGACGTGGTATTCCAGTACGAGCCGGTc                       >  1:2692569/1‑148 (MQ=255)
                                                                                                                              gAAGCGATTACTCAGGCGGTGATTGGTCGTCCGATCAACTTCCAGGGGCTGGGCGGTGATGAAGCAAACACCCAGGCGCAAGGGATTCTGGAACGCGCGGCGAAGCGTGCCGGATTCAGGGACGTGGTATTCCAGTACGAGCCGGTcg                      >  2:2064339/1‑148 (MQ=255)
                                                                                                                                   gATTACTCAGGCGGTGATTGGTCGTCCGATCAACTTCCAGGGGCTGGGCGGTGATGAAGCAAACACCCAGGCGCAAGGGATTCTGGAACGCGCGGCGAAGCGTGCCGGATTCAGGGACGTGGTATTCCAGTACGAGCCGGTCGCggct                 >  2:2922726/1‑148 (MQ=255)
                                                                                                                                    aTTACTCAGGCGGTTATTGGTCGTCCGATCAACTTCCAGGGGCTGGGCGGTGATGAAGCAAACACCCAGGCGCNAGGGATTCTGGAACGCGCGGCGAAGCGTGCCGGATTCAGGGACGTGGTATTCCAGTACGAGCCGGTCGCggctg                >  1:1680404/1‑148 (MQ=255)
                                                                                                                                     ttACTCAGGCGGTTATTGGTCGTCCGATCAACTTCCAGGGGCTGGGCGGTGATGAAGCAAACACCCAGGCGCAAGGGATTCTGGAACGCGCGGCGAAGCGTGCCGGATTCAGGGACGTGGTATTCCAGTACGAGCCGGTCGCggctgg               >  2:2636735/1‑148 (MQ=255)
                                                                                                                                           aGGCGGTGATTGGTCGTCCGATCAACTTCCAGGGGCTGGGCGGTGATGAAGCAAACACCCAGGCGCAAGGGATTCTGGAACGCGCGGCGAAGCGTGCCGGATTCAGGGACGTGGTATTCCAGTACGAGCCGGTCGCGGCTGGGCTGGa         >  2:2719868/1‑148 (MQ=255)
                                                                                                                                            ggCGGTGATTGGTCGTCCGATCAACTTCCAGGGGCTGGGCGGTGATGAAGCAAACACCCAGGCGCAAGGGATTCTGGAACGCGCGGCGAAGCGTGCCGGATTCAGGGACGTGGTATTCCAGTACGAGCCGGTCGCGGCTGGGCTGGAt        <  1:1900921/148‑1 (MQ=255)
                                                                                                                                              cGGTGATTGGTCGTCCGATCAACTTCCAGGGGCTGGGCGGTGATGAAGCAAACACCCAGGCGCAAGGGATTCTGGAACGCGCGGCGAAGCGTGCCGGATTCAGGGACGTGGTATTCCAGTACGAGCCGGTCGCGGCTGGGCTGGATTa      <  2:3253963/148‑1 (MQ=255)
                                                                                                                                                gTGATTGGTCGTCCGATCAACTTCCAGGGGCTGGGCGGTGATGAAGCAAACACCCAGGCGCAAGGGATTCTGGAACGCGCGGCGAAGCGTGCCGGATTCAGGGACGTGGTATTCCAGTACGAGCCGGTCGCGGCTGGGCTGGATTACg    <  1:677699/148‑1 (MQ=255)
                                                                                                                                                  gATTGGTCGTCCGATAAACTTCCAGGGGCTGGGCGGTGATGAAGCAAACACCCAGGCGCAAGGGATTCTGTAAAGCGCGGCGAAGCGTGCCGGATTCAGGGACGTGGTATTCCAGTACGAGCCGGTCGCGGCTGGGCTGGATTACGaa  >  2:1420229/1‑148 (MQ=255)
                                                                                                                                                  |                                                                                                                                                   
TTTGTGAAATCACCAAAATCGTTCCTCGGTGCCAGCGGCTTAAAACCGCAGCAGGTAGCGCTGTTTGAGGATCTGGTCTGCGCAATGATGTTGCACATTCGCCAGCAGGCGCAGGCACAGCTGCCAGAAGCGATTACTCAGGCGGTGATTGGTCGTCCGATCAACTTCCAGGGGCTGGGCGGTGATGAAGCAAACACCCAGGCGCAAGGGATTCTGGAACGCGCGGCGAAGCGTGCCGGATTCAGGGACGTGGTATTCCAGTACGAGCCGGTCGCGGCTGGGCTGGATTACGAA  >  CP009273/2141467‑2141760

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A