Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F309 I0 R1 1697 197.2 6627756 95.7% 6342762 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,241,222 T→G 86.8% I264L (ATT→CTT)  lysP ← lysine transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,241,2220TG86.8% 585.9 / 46.7 182I264L (ATT→CTT) lysPlysine transporter
Reads supporting (aligned to +/- strand):  ref base T (15/9);  new base G (74/84);  total (89/93)
Fisher's exact test for biased strand distribution p-value = 1.90e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.85e-01

Only 100 of 184 total aligned reads displayed.

CCCGCTGACAGCACCGCCGTCAGAATAACTGCGTTCATCACCGCCGCCGCAGAGAGCAGACCCGCGTGCTGGAACACCAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAATAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTGGAATGTTTTTCGCCGGATCTTCGGACTCGCCTGCAGCAATACCGATCAGCTCGGTTCCCTGGAAAGAGA  >  CP009273/2241077‑2241365
                                                                                                                                                 |                                                                                                                                               
cccGCTGACAGCACCGCCGTCAGAATAACTGCGTTCATCACCGCCGCCGCAGAGAGCAGACCCGCGTGCTGGAACACCAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGgaagaa                                                                                                                                               <  1:2350938/148‑1 (MQ=255)
  cGCTGACAGCACCGCCGTCAGAATAACTGCGTTCATCACCGCCGCCGCAGAGAGCAGACCCGCGTGCTGGAACACCAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATc                                                                                                                                             <  1:582544/148‑1 (MQ=255)
      gACAGCACCGCCGTCAGAATAACTGCGTTCATCACCGCCGCCGCAGAGAGCAGACCCGCGTGCTGGAACACCAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGc                                                                                                                                         <  1:174096/148‑1 (MQ=255)
         aGCACCGCCGTCAGAATAACTGCGTTCATCACCGCCGCCGCAGAGAGCAGACCCGCGTGCTGGAACACCAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGa                                                                                                                                      <  1:2674123/148‑1 (MQ=255)
         aGCACCGCCGTCAGAATAACTGCGTTCATCACCGCCGCCGCAGAGAGCAGACCCGCGTGCTGGAACACCAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGa                                                                                                                                      <  1:2461259/148‑1 (MQ=255)
          gCACCGCCGTCAGAATAACTGCGTTCATCACCGCCGCCGCAGAGAGCAGACCCGCGTGCTGGAACACCAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGAt                                                                                                                                     <  1:774642/148‑1 (MQ=255)
          gCACCGCCGTCAGAATAACTGCGTTCATCACCGCCGCCGCAGAGAGCAGACCCGCGTGCTGGAACACCAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGAt                                                                                                                                     >  2:2923922/1‑148 (MQ=255)
           cACCGCCGTCAGAATAACTGCGTTCATCACCGCCGCCGCAGAGAGCAGACCCGCGTGCTGGAACACCAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAATAATCAGGCTGATa                                                                                                                                    <  1:738657/148‑1 (MQ=255)
           cACCGCCGTCAGAATAACTGCGTTCATCACCGCCGCCGCAGAGAGCAGACCCGCGTGCTGGAACACCAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATa                                                                                                                                    <  2:2732693/148‑1 (MQ=255)
           cACCGCCGTCAGAATAACTGCGTTCATCACCGCCGCCGCAGAGAGCAGACCCGCGTGCTGGAACACCAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATa                                                                                                                                    <  1:949544/148‑1 (MQ=255)
            aCCGCCGTCAGAATAACTGCGTTCATCACCGCCGCCGCAGAGAGCAGACCCGCGTGCTGGAACACCAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATaa                                                                                                                                   <  2:390945/148‑1 (MQ=255)
            aCCGCCGTCAGAATAACTGCGTTCATCACCGCCGCCGCAGAGAGCAGACCCGCGTGCTGGAACACCAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATaa                                                                                                                                   >  1:2963220/1‑148 (MQ=255)
            aCCGCCGTCAGAATAACTGCGTTCATCACCGCCGCCGCAGAGAGCAGACCCGCGTGCTGGAACACCAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATaa                                                                                                                                   <  2:2285606/148‑1 (MQ=255)
            aCCGCCGTCAGAATAACTGCGTTCATCACCGCCGCCGCAGAGAGCAGACCCGCGTGCTGGAACACCAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATaa                                                                                                                                   <  2:2285382/148‑1 (MQ=255)
              cgccgTCAGAATAACTGCGTTCATCACCGCCGCCGCAGAGAGCAGACCCGCGTGCTGGAACACCAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATc                                                                                                                                 <  1:343103/148‑1 (MQ=255)
               gccgTCAGAATAACTGCGTTCATCACCGCCGCCGCAGAGAGCAGACCCGCGTGCTGGAACACCAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCa                                                                                                                                <  1:1632763/148‑1 (MQ=255)
                  gTCAGAATAACTGCGTTCATCACCGCCGCCGCAGAGAGCAGACCCGCGTGCTGGAACACCAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGa                                                                                                                             <  1:3068986/148‑1 (MQ=255)
                   tCAGAATAACTGCGTTCATCACCGCCGCCGCAGAGAGCAGACCCGCGTGCTGGAACACCAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAATAATCAGGCTGATAATCAGGAt                                                                                                                            <  2:1359076/148‑1 (MQ=255)
                   tCAGAATAACTGCGTTCATCACCGCCGCCGCAGAGAGCAGACCCGCGTGCTGGAACACCAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAATAATCAGGCTGATAATCAGGAt                                                                                                                            <  2:1359093/148‑1 (MQ=255)
                   tCAGAATAACTGCGTTCATCACCGCCGCCGCAGAGAGCAGACCCGCGTGCTGGAACACCAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGAt                                                                                                                            >  2:2661487/1‑148 (MQ=255)
                      gAATAACTGCGTTCATCACCGCCGCCGCAGAGAGCAGACCCGCGTGCTGGAACACCAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATcgc                                                                                                                         >  1:1507265/1‑148 (MQ=255)
                       aaTAACTGCGTTCATCACCGCCGCCGCAGAGAGCAGACCCGCGTGCTGGAACACCAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATcgcg                                                                                                                        >  1:1318125/1‑148 (MQ=255)
                        aTAACTGCGTTCATCACCGCCGCCGCAGAGAGCAGACCCGCGTGCTGGAACACCAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGa                                                                                                                       <  1:561316/148‑1 (MQ=255)
                        aTAACTGCGTTCATCACCGCCGCCGCAGAGAGCAGACCCGCGTGCTGGAACACCAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGATACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGa                                                                                                                       >  1:2747243/1‑148 (MQ=255)
                             tGCGTTCATCACCGCCGCCGCAGAGAGCAGACCCGCGTGCTGGAACACCAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAAcaca                                                                                                                  >  2:765528/1‑148 (MQ=255)
                              gCGTTCATCACCGCCGCCGCAGAGAGCAGACCCGCGTGCTGGAACACCAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACTGAAGAATCAGGCTGATAATCAGGATCGCGAACACAt                                                                                                                 <  1:2094121/148‑1 (MQ=255)
                               cGTTCATCACCGCCGCCGCAGAGAGCAGACCCGCGTGCTGGAACACCAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATa                                                                                                                >  1:2873740/1‑148 (MQ=255)
                                gTTCATCACCGCCGCCGCAGAGAGCAGACCCGCGTGCTGGAACACCAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAg                                                                                                               >  1:2891162/1‑148 (MQ=255)
                                 ttCATCACCGCCGCCGCAGAGAGCAGACCCGCGTGCTGGAACACCAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGa                                                                                                              <  2:631830/148‑1 (MQ=255)
                                  tcatcaCCGCCGCCGCAGAGAGCAGACCCGCGTGCTGGAACACCAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGaa                                                                                                             >  1:2291465/1‑148 (MQ=255)
                                    atcaCCGCCGCCGCAGAGAGCAGACCCGCGTGCTGGAACACCAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGaaca                                                                                                           <  1:1288625/148‑1 (MQ=255)
                                     tcaCCGCCGCCGCAGAGAGCAGACCCGCGTGCTGGAACACCAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGaacaa                                                                                                          >  1:804662/1‑148 (MQ=255)
                                     tcaCCGCCGCCGCAGAGAGCAGACCCGCGTGCTGGAACACCAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGaacaa                                                                                                          <  2:2458727/148‑1 (MQ=255)
                                      caCCGCCGCCGCAGAGAGCAGACCCGCGTGCTGGAACACCAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGaacaac                                                                                                         <  1:2807072/148‑1 (MQ=255)
                                       aacgccgcctcTGAGAGCAGACCCGCTTGCTTGAACACCAGTGTTAACGGACTATCGCTGATGTCTTTATAATCGTTACGCAGCAGGCTCGGATCGGTGTACTGAAGAATCAGGCTGATAATCAGTATAGCGAAAACATAGatcaact                                                                                                        <  2:1071322/146‑2 (MQ=255)
                                       aCCGCCGCCGCAGAGAGCAGACCCGCGTGCTGGAACACCAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAacaaca                                                                                                        <  1:963091/148‑1 (MQ=255)
                                        ccgccgccgCAGAGAGCAGACCCGCGTGCTGGAACACCAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAg                                                                                                       <  2:3220553/148‑1 (MQ=255)
                                                gcAGAGAGCAGACCCGCGTGCTGGAACACCAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGcc                                                                                               <  1:587132/148‑1 (MQ=255)
                                                       gCAGACCCGCGTGCTGGAACACCAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAacac                                                                                        <  1:1233431/148‑1 (MQ=255)
                                                           aCCCGCGTGCTGGAACACCAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGa                                                                                    >  1:2784629/1‑148 (MQ=255)
                                                           aCCCGCGTGCTGGAACACCAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGa                                                                                    >  1:651326/1‑148 (MQ=255)
                                                              cgcgTGCTGGAACACCAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGt                                                                                 <  1:1148381/148‑1 (MQ=255)
                                                               gcgTGCTGGAACACCAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTa                                                                                <  2:1849304/148‑1 (MQ=255)
                                                                    cTGGAACACCAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACcgcg                                                                           <  1:1530920/148‑1 (MQ=255)
                                                                    cTGGAACACCAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACcgcg                                                                           <  1:1246422/148‑1 (MQ=255)
                                                                        aaCACCAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTg                                                                       >  2:1261545/1‑148 (MQ=255)
                                                                         acacCAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTgg                                                                      >  2:12271/1‑148 (MQ=255)
                                                                         acacCAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTgg                                                                      >  2:12685/1‑148 (MQ=255)
                                                                          cacCAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTGGa                                                                     <  1:1123544/148‑1 (MQ=255)
                                                                           acCAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTGGaa                                                                    <  1:3152634/148‑1 (MQ=255)
                                                                             cAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTGGACTg                                                                  >  2:1801737/1‑148 (MQ=255)
                                                                               gggTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTGGAATGtt                                                                <  2:459698/148‑1 (MQ=255)
                                                                                ggTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAATAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTGGAATGttt                                                               >  2:3168498/1‑148 (MQ=255)
                                                                                ggTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTGGACTGttt                                                               >  2:2890032/1‑148 (MQ=255)
                                                                                ggTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTGGAATGttt                                                               <  2:1165074/148‑1 (MQ=255)
                                                                                ggTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTGGAATGttt                                                               <  2:2815978/148‑1 (MQ=255)
                                                                                 gTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTGGAATGtttt                                                              <  1:569389/148‑1 (MQ=255)
                                                                                    aaCGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTGGAATGTTTTTCg                                                           <  2:1904562/148‑1 (MQ=255)
                                                                                    aaCGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTGGAATGTTTTTCg                                                           >  1:2973896/1‑148 (MQ=255)
                                                                                    aaCGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTGGAATGTTTTTCg                                                           <  2:235868/148‑1 (MQ=255)
                                                                                         aCTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAATAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTGGAATGTTTTTCGCCGGa                                                      >  2:1814821/1‑148 (MQ=255)
                                                                                         aCTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAATAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTGGAATGTTTTTCGCCGGa                                                      >  2:1652491/1‑148 (MQ=255)
                                                                                         aCTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAATAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTGGAATGTTTTTCGCCGGa                                                      >  1:2579429/1‑148 (MQ=255)
                                                                                         aCTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTGGAATGTTTTTCGCCGGa                                                      >  2:971408/1‑148 (MQ=255)
                                                                                          cTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTGGAATGTTTTTCGCCGGAt                                                     <  1:1950775/148‑1 (MQ=255)
                                                                                          cTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTGGAATGTTTTTCGCCGGAt                                                     <  1:2306260/148‑1 (MQ=255)
                                                                                            aaCGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTGGAATGTTTTTCGCCGGATCt                                                   <  2:597929/148‑1 (MQ=255)
                                                                                             aCGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTGGAATGTTTTTCGCCGGATCtt                                                  <  2:1248864/148‑1 (MQ=255)
                                                                                                 tGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTGGAATGTTTTTCGCCGGATCTTCGGa                                              <  2:1318125/148‑1 (MQ=255)
                                                                                                 tGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTGGAATGTTTTTCGCCGGATCTTCGGa                                              <  1:140304/148‑1 (MQ=255)
                                                                                                     gTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAATAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTGGAATGTTTTTCGCCGGATCTTCGGACTCg                                          >  2:1086677/1‑148 (MQ=255)
                                                                                                      tCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTGGAATGTTTTTCGCCGGATCTTCGGACTCGc                                         <  2:386824/148‑1 (MQ=255)
                                                                                                            aCATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTGGAATGTTTTTCGCCGGATCTTCGGACTCGCCTgcag                                   >  2:1619257/1‑148 (MQ=255)
                                                                                                              aTCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTGGAATGTTTTTCGCCGGATCTTCGGACTCGCCTgcagca                                 <  1:1654724/148‑1 (MQ=255)
                                                                                                              aTCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTGGAATGTTTTTCGCCGGATCTTCGGACTCGCCTgcagca                                 >  2:3178894/1‑148 (MQ=255)
                                                                                                                cGTTACGCAGCAGGCTCTGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTGGAATGTTTTTCGCCGGATCTTCGGACTCGCCTGCAGCAAt                               <  2:3029753/148‑1 (MQ=255)
                                                                                                                cGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTGGAATGTTTTTCGCCGGATCTTCGGACTCGCCTGCAGCAAt                               >  1:1154542/1‑148 (MQ=255)
                                                                                                                cGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTGGAATGTTTTTCGCCGGATCTTCGGACTCGCCTGCAGCAAt                               >  1:1846571/1‑148 (MQ=255)
                                                                                                                      gcagcaGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTGGAATGTTTTTCGCCGGATCTTCGGACTCGCCTGCAGCAATACCGAt                         <  1:1088426/148‑1 (MQ=255)
                                                                                                                         gcagGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTGGAATGTTTTTCGCCGGATCTTCGGACTCGCCTGCAGCAATCCCGATCAg                      >  1:195434/1‑148 (MQ=255)
                                                                                                                          cagGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTGGAATGTTTTTCGCCGGATCTTCGGACTCGCCTGCAGCAATACCGATCAGc                     >  1:2039493/1‑148 (MQ=255)
                                                                                                                            ggCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTGGAATGTTTTTCGCCGGATCTTCGGACTCGCCTGCAGCAATACCGATCAGCTc                   >  1:2897895/1‑148 (MQ=255)
                                                                                                                             gCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTGGAATGTTTTTCGCCGGATCTTCGGACTCGCCTGCAGCAATACCGATCAGCTCg                  >  2:2046581/1‑148 (MQ=255)
                                                                                                                             gCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTGGAATGTTTTTCGCCGGATCTTCGGACTCGCCTGCAGCAATACCGATCAGCTCg                  >  1:2415464/1‑148 (MQ=255)
                                                                                                                              cTCGGATCGGTGTACGGACGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTGGAATGTTTTTCGCCGGATCTTCGGACTCGCCTGCAGCAATACCGATCAGCTCgg                 >  2:1533113/1‑148 (MQ=255)
                                                                                                                              cTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTGGAATGTTTTTCGCCGGATCTTCGGACTCGCCTGCAGCAATACCGATCAGCTCgg                 >  2:3174110/1‑148 (MQ=255)
                                                                                                                              cTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTGGAATGTTTTTCGCCGGATCTTCGGACTCGCCTGCAGCAATACCGATCAGCTCgg                 >  2:1033458/1‑148 (MQ=255)
                                                                                                                              cTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTGGAATGTTTTTCGCCGGATCTTCGGACTCGCCTGCAGCAATACCGATCAGCTCgg                 <  2:2967163/148‑1 (MQ=255)
                                                                                                                                  gATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTGGAATGTTTTTCGCCGGATCTTCGGACTCGCCTGCAGCAATACCGATCAGCTCGGTTcc             <  1:1161443/148‑1 (MQ=255)
                                                                                                                                   aTCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTGGAATGTTTTTCGCCGGATCTTCGGACTCGCCTGCAGCAATACCGATCAGCTCGGTTccc            <  1:82779/148‑1 (MQ=255)
                                                                                                                                   aTCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTGGAATGTTTTTCGCCGGATCTTCGGACTCGCCTGCAGCAATACCGATCAGCTCGGTTccc            >  2:205569/1‑148 (MQ=255)
                                                                                                                                    tCGGTGTACGGAATAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTGGAATGTTTTTCGCCGGATCTTCGGACTCGCCTGCAGCAATACCGATCAGCTCGGTTCCCt           <  1:958296/148‑1 (MQ=255)
                                                                                                                                    tCGGTGTACGGAATAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTGGAATGTTTTTCGCCGGATCTTCGGACTCGCCTGCAGCAATACCGATCAGCTCGGTTCCCt           >  2:1785391/1‑148 (MQ=255)
                                                                                                                                       gtgtACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTGGAATGTTTTTCGCCGGATCTTCGGACTCGCCTGCAGCAATACCGATCAGCTCGGTTCCCTGGa        >  1:2646780/1‑148 (MQ=255)
                                                                                                                                        tgtACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTGGAATGTTTTTCGCCGGATCTTCGGACTCGCCTGCAGCAATACCGATCAGCTCGGTTCCCTGGaa       <  1:1763566/148‑1 (MQ=255)
                                                                                                                                         gtACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTGGAATGTTTTTCGCCGGATCTTCGGACTCGCCTGCAGCAATACCGATCAGCTCGGTTCCCTGGaaa      >  2:1243760/1‑148 (MQ=255)
                                                                                                                                          tACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTGGAATGTTTTTCGCCGGATCTTCGGACTCGCCTGCAGCAATACCGATCAGCTCGGTTCCCTGGAAag     <  2:2291139/148‑1 (MQ=255)
                                                                                                                                           aCGGAATAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTGGAATGTTTTTCGCCGGATCTTCGGACTCGCCTGCAGCAATACCGATCAGCTCGGTTCCCTGGAAaga    >  2:2671504/1‑148 (MQ=255)
                                                                                                                                             ggAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTGGAATGTTTTTCGCCGGATCTTCGGACTCGCCTGCAGCAATACCGATCAGCTCGGTTCCCTGGAAAgaga  >  2:3127643/1‑148 (MQ=255)
                                                                                                                                             ggAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTGGAATGTTTTTCGCCGGATCTTCGGACTCGCCTGCAGCAATACCGATCAGCTCGGTTCCCTGGAAAgaga  <  2:3295197/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                               
CCCGCTGACAGCACCGCCGTCAGAATAACTGCGTTCATCACCGCCGCCGCAGAGAGCAGACCCGCGTGCTGGAACACCAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAATAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTGGAATGTTTTTCGCCGGATCTTCGGACTCGCCTGCAGCAATACCGATCAGCTCGGTTCCCTGGAAAGAGA  >  CP009273/2241077‑2241365

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TTACCCGCTGACAGCACCGCCGTCAGAATAACTGCGTTCATCACCGCCGCCGCAGAGAGCAGACCCGCGTGCTGGAACACCAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAATAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTGGAATGTTTTTCGCCGGATCTTCGGACTCGCCTGCAGCAATACCGATCAGCTCGGTTCCCTGGAAAGAGAAGCCGA  >  CP009273/2241074‑2241371
                                                                                                                                                    |                                                                                                                                                     
TTACCCGCTGACAGCACCGCCGTCAGAATAACTGCGTTCATCACCGCCGCCGCAGAGAGCAGACCCGCGTGCTGGAACACCAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGA                                                                                                                                                      >  SRR19973070.3110590/1‑150 (MQ=60)
   CCCGCTGACAGCACCGCCGTCAGAATAACTGCGTTCATCACCGCCGCCGCAGAGAGCAGACCCGCGTGCTGGAACACCAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATC                                                                                                                                                   <  SRR19973070.3334385/150‑1 (MQ=60)
   CCCGCTGACAGCACCGCCGTCAGAATAACTGCGTTCATCACCGCCGCCGCAGAGAGCAGACCCGCGTGCTGGAACACCAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATC                                                                                                                                                   <  SRR19973070.2366522/150‑1 (MQ=60)
     CGCTGACAGCACCGCCGTCAGAATAACTGCGTTCATCACCGCCGCCGCAGAGAGCAGACCCGCGTGCTGGAACACCAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAG                                                                                                                                                 <  SRR19973070.2667922/150‑1 (MQ=60)
     CGCTGACAGCACCGCCGTCAGAATAACTGCGTTCATCACCGCCGCCGCAGAGAGCAGACCCGCGTGCTGGAACACCAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAG                                                                                                                                                 <  SRR19973070.586559/150‑1 (MQ=60)
     CGCTGACAGCACCGCCGTCAGAATAACTGCGTTCATCACCGCCGCCGCAGAGAGCAGACCCGCGTGCTGGAACACCAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAG                                                                                                                                                 <  SRR19973070.587903/150‑1 (MQ=60)
      GCTGACAGCACCGCCGTCAGAATAACTGCGTTCATCACCGCCGCCGCAGAGAGCAGACCCGCGTGCTGGAACACCAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGG                                                                                                                                                <  SRR19973070.770570/150‑1 (MQ=60)
       CTGACAGCACCGCCGTCAGAATAACTGCGTTCATCACCGCCGCCGCAGAGAGCAGACCCGCGTGCTGGAACACCAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGC                                                                                                                                               >  SRR19973070.1567115/1‑150 (MQ=60)
       CTGACAGCACCGCCGTCAGAATAACTGCGTTCATCACCGCCGCCGCAGAGAGCAGACCCGCGTGCTGGAACACCAGGGTGAAAGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAATAATCAGGC                                                                                                                                               <  SRR19973070.2730481/150‑1 (MQ=60)
         GACAGCACCGCCGTCAGAATAACTGCGTTCATCACCGCCGCCGCAGAGAGCAGACCCGCGTGCTGGAACACCAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTG                                                                                                                                             <  SRR19973070.175482/150‑1 (MQ=60)
            AGCACCGCCGTCAGAATAACTGCGTTCATCACCGCCGCCGCAGAGAGCAGACCCGCGTGCTGGAACACCAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATA                                                                                                                                          <  SRR19973070.2477331/150‑1 (MQ=60)
            AGCACCGCCGTCAGAATAACTGCGTTCATCACCGCCGCCGCAGAGAGCAGACCCGCGTGCTGGAACACCAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATA                                                                                                                                          <  SRR19973070.2691487/150‑1 (MQ=60)
             GCACCGCCGTCAGAATAACTGCGTTCATCACCGCCGCCGCAGAGAGCAGACCCGCGTGCTGGAACACCAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAA                                                                                                                                         >  SRR19973070.2982274/1‑150 (MQ=60)
             GCACCGCCGTCAGAATAACTGCGTTCATCACCGCCGCCGCAGAGAGCAGACCCGCGTGCTGGAACACCAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAA                                                                                                                                         >  SRR19973070.2986229/1‑150 (MQ=60)
             GCACCGCCGTCAGAATAACTGCGTTCATCACCGCCGCCGCAGAGAGCAGACCCGCGTGCTGGAACACCAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAA                                                                                                                                         <  SRR19973070.780066/150‑1 (MQ=60)
              CACCGCCGTCAGAATAACTGCGTTCATCACCGCCGCCGCAGAGAGCAGACCCGCGTGCTGGAACACCAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAATAATCAGGCTGATAAT                                                                                                                                        <  SRR19973070.743882/150‑1 (MQ=60)
              CACCGCCGTCAGAATAACTGCGTTCATCACCGCCGCCGCAGAGAGCAGACCCGCGTGCTGGAACACCAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAAT                                                                                                                                        >  SRR19973070.2342269/1‑150 (MQ=60)
              CACCGCCGTCAGAATAACTGCGTTCATCACCGCCGCCGCAGAGAGCAGACCCGCGTGCTGGAACACCAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAAT                                                                                                                                        <  SRR19973070.956311/150‑1 (MQ=60)
                 CGCCGTCAGAATAACTGCGTTCATCACCGCCGCCGCAGAGAGCAGACCCGCGTGCTGGAACACCAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAG                                                                                                                                     <  SRR19973070.345607/150‑1 (MQ=60)
                  GCCGTCAGAATAACTGCGTTCATCACCGCCGCCGCAGAGAGCAGACCCGCGTGCTGGAACACCAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGG                                                                                                                                    <  SRR19973070.1644087/150‑1 (MQ=60)
                    CGTCAGAATAACTGCGTTCATCACCGCCGCCGCAGAGAGCAGACCCGCGTGCTGGAACACCAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTCACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGAT                                                                                                                                  >  SRR19973070.87302/1‑150 (MQ=60)
                     GTCAGAATAACTGCGTTCATCACCGCCGCCGCAGAGAGCAGACCCGCGTGCTGGAACACCAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATC                                                                                                                                 <  SRR19973070.3088673/150‑1 (MQ=60)
                       CAGAATAACTGCGTTCATCACCGCCGCCGCAGAGAGCAGACCCGCGTGCTGGAACACCAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAATAATCAGGCTGATAATCAGGATCGC                                                                                                                               >  SRR19973070.2320445/1‑150 (MQ=60)
                       CAGAATAACTGCGTTCATCACCGCCGCCGCAGAGAGCAGACCCGCGTGCTGGAACACCAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGC                                                                                                                               >  SRR19973070.1518456/1‑150 (MQ=60)
                       CAGAATAACTGCGTTCATCACCGCCGCCGCAGAGAGCAGACCCGCGTGCTGGAACACCAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGC                                                                                                                               >  SRR19973070.1517810/1‑150 (MQ=60)
                        AGAATAACTGCGTTCATCACCGCCGCCGCAGAGAGCAGACCCGCGTGCTGGAACACCAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCG                                                                                                                              >  SRR19973070.1327247/1‑150 (MQ=60)
                        AGAATAACTGCGTTCATCACCGCCGCCGCAGAGAGCAGACCCGCGTGCTGGAACACCAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCG                                                                                                                              <  SRR19973070.1351816/150‑1 (MQ=60)
                         GAATAACTGCGTTCATCACCGCCGCCGCAGAGAGCAGACCCGCGTGCTGGAACACCAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGA                                                                                                                             >  SRR19973070.2765112/1‑150 (MQ=60)
                         GAATAACTGCGTTCATCACCGCCGCCGCAGAGAGCAGACCCGCGTGCTGGAACACCAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGATACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGA                                                                                                                             >  SRR19973070.2764900/1‑150 (MQ=60)
                           ATAACTGCGTTCATCACCGCCGCCGCAGAGAGCAGACCCGCGTGCTGGAACACCAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAAC                                                                                                                           <  SRR19973070.565193/150‑1 (MQ=60)
                           ATAACTGCGTTCATCACCGCCGCCGCAGAGAGCAGACCCGCGTGCTGGAACACCAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAAC                                                                                                                           <  SRR19973070.565027/150‑1 (MQ=60)
                               CTGCGTTCATCACCGCCGCCGCAGAGAGCAGACCCGCGTGCTGGAACACCAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAATAATCAGGCTGATAATCAGGATCGCGAACACAT                                                                                                                       >  SRR19973070.299607/1‑150 (MQ=60)
                                TGCGTTCATCACCGCCGCCGCAGAGAGCAGACCCGCGTGCTGGAACACCAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATA                                                                                                                      >  SRR19973070.2892359/1‑150 (MQ=60)
                                 GCGTTCATCACCGCCGCCGCAGAGAGCAGACCCGCGTGCTGGAACACCAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACTGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAG                                                                                                                     <  SRR19973070.2108198/150‑1 (MQ=60)
                                 GCGTTCATCACCGCCGCCGCAGAGAGCAGACCCGCGTGCTGGAACACCAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAG                                                                                                                     >  SRR19973070.2909873/1‑150 (MQ=60)
                                 GCGTTCATCACCGCCGCCGCAGAGAGCAGACCCGCGTGCTGGAACACCAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAG                                                                                                                     >  SRR19973070.2910512/1‑150 (MQ=60)
                                   GTTCATCACCGCCGCCGCAGAGAGCAGACCCGCGTGCTGGAACACCAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAA                                                                                                                   >  SRR19973070.2306372/1‑150 (MQ=60)
                                   GTTCATCACCGCCGCCGCAGAGAGCAGACCCGCGTGCTGGAACACCAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAA                                                                                                                   >  SRR19973070.2306699/1‑150 (MQ=60)
                                    TTCATCACCGCCGCCGCAGAGAGCAGACCCGCGTGCTGGAACACCAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAGCACATAGAAC                                                                                                                  <  SRR19973070.1316420/150‑1 (MQ=60)
                                      CATCACCGCCGCCGCAGAGAGCAGACCCGCGTGCTGGAACACCAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAA                                                                                                                >  SRR19973070.810197/1‑150 (MQ=60)
                                      CATCACCGCCGCCGCAGAGAGCAGACCCGCGTGCTGGAACACCAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAA                                                                                                                >  SRR19973070.810145/1‑150 (MQ=60)
                                       ATCACCGCCGCCGCAGAGAGCAGACCCGCGTGCTGGAACACCAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAAC                                                                                                               <  SRR19973070.1297609/150‑1 (MQ=60)
                                         CACCGCCGCCGCAGAGAGCAGACCCGCGTGCTGGAACACCAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAG                                                                                                             <  SRR19973070.2825214/150‑1 (MQ=60)
                                          ACCGCCGCCGCAGAGAGCAGACCCGCGTGCTGGAACACCAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGG                                                                                                            <  SRR19973070.969969/150‑1 (MQ=60)
                                          ACCGCCGCCGCAGAGAGCAGACCCGCGTGCTGGAACACCAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGG                                                                                                            <  SRR19973070.1250698/150‑1 (MQ=60)
                                                  CGCAGAGAGCAGACCCGCGTGCTGGAACACCAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCACCAGTCTCGGATCGGTGTACGGAATAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCA                                                                                                    <  SRR19973070.1966635/150‑1 (MQ=60)
                                                   GCAGAGAGCAGACCCGCGTGCTGGAACACCAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAG                                                                                                   <  SRR19973070.591185/150‑1 (MQ=60)
                                                         AGCAGACCCGCGTGCTGGAACACCAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGTCAGAACACC                                                                                             <  SRR19973070.824251/150‑1 (MQ=60)
                                                          GCAGACCCGCGTGCTGGAACACCAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCT                                                                                            <  SRR19973070.1241956/150‑1 (MQ=60)
                                                          GCAGACCCGCGTGCTGGAACACCAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCT                                                                                            <  SRR19973070.1242069/150‑1 (MQ=60)
                                                            AGACCCGCGTGCTGGAACACCAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGA                                                                                          >  SRR19973070.2802541/1‑150 (MQ=60)
                                                            AGACCCGCGTGCTGGAACACCAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGA                                                                                          >  SRR19973070.655973/1‑150 (MQ=60)
                                                                 CGCGTGCTGGAACACCAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTAC                                                                                     <  SRR19973070.1156374/150‑1 (MQ=60)
                                                                 CGCGTGCTGGAACACCAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTAC                                                                                     <  SRR19973070.1156553/150‑1 (MQ=60)
                                                                 CGCGTGCTGGAACACCAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTAC                                                                                     >  SRR19973070.691329/1‑150 (MQ=60)
                                                                       CTGGAACACCAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCG                                                                               <  SRR19973070.1255158/150‑1 (MQ=60)
                                                                       CTGGAACACCAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCG                                                                               <  SRR19973070.1541670/150‑1 (MQ=60)
                                                                           AACACCAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTGGA                                                                           >  SRR19973070.2907339/1‑150 (MQ=60)
                                                                             CACCAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTGGAAT                                                                         <  SRR19973070.1131257/150‑1 (MQ=60)
                                                                              ACCAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTGGAATG                                                                        <  SRR19973070.3173005/150‑1 (MQ=60)
                                                                                    GTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTGGAATGTTTTTC                                                                  >  SRR19973070.2067236/1‑150 (MQ=60)
                                                                                    GTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTGGAATGTTTTTC                                                                  <  SRR19973070.573369/150‑1 (MQ=60)
                                                                                    GTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTGGAATGTTTTTC                                                                  <  SRR19973070.573237/150‑1 (MQ=60)
                                                                                     TGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTGGAATGTTTTTCG                                                                 >  SRR19973070.2992992/1‑150 (MQ=60)
                                                                                          GGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAATAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTGGAATGTTTTTCGCCGGA                                                            >  SRR19973070.2596291/1‑150 (MQ=60)
                                                                                             CTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTGGAATGTTTTTCGCCGGATCT                                                         <  SRR19973070.1963041/150‑1 (MQ=60)
                                                                                             CTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTGGAATGTTTTTCGCCGGATCT                                                         <  SRR19973070.1963879/150‑1 (MQ=60)
                                                                                             CTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTGGAATGTTTTTCGCCGGATCT                                                         <  SRR19973070.2321595/150‑1 (MQ=60)
                                                                                              TAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTGGAATGTTTTTCGCCGGATCTT                                                        <  SRR19973070.2808162/150‑1 (MQ=60)
                                                                                              TAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTGGAATGTTTTTCGCCGGATCTT                                                        <  SRR19973070.2648858/150‑1 (MQ=60)
                                                                                               AACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAATAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTGGAATGTTTTTCGCCGGATCTTC                                                       <  SRR19973070.517253/150‑1 (MQ=60)
                                                                                                   CTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTGGAATGTTTTTCGCCGGATCTTCGGAC                                                   >  SRR19973070.186085/1‑150 (MQ=60)
                                                                                                    TGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTGGAATGTTTTTCGCCGGATCTTCGGACT                                                  <  SRR19973070.142172/150‑1 (MQ=60)
                                                                                                    TGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTGGAATGTTTTTCGCCGGATCTTCGGACT                                                  <  SRR19973070.141525/150‑1 (MQ=60)
                                                                                                             TAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTGGAATGTTTTTCGCCGGATCTTCGGACTCGCCTGCAG                                         <  SRR19973070.1703888/150‑1 (MQ=60)
                                                                                                                 ATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTGGAATGTTTTTCGCCGGATCTTCGGACTCGCCTGCAGCAAT                                     >  SRR19973070.1859237/1‑150 (MQ=60)
                                                                                                                 ATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTGGAATGTTTTTCGCCGGATCTTCGGACTCGCCTGCAGCAAT                                     >  SRR19973070.2398219/1‑150 (MQ=60)
                                                                                                                 ATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTGGAATGTTTTTCGCCGGATCTTCGGACTCGCCTGCAGCAAT                                     <  SRR19973070.1666132/150‑1 (MQ=60)
                                                                                                                 ATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTGGAATGTTTTTCGCCGGATCTTCGGACTCGCCTGCAGCAAT                                     >  SRR19973070.1162599/1‑150 (MQ=60)
                                                                                                                         GCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTGGAATGTTTTTCGCCGGATCTTCGGACTCGCCTGCAGCAATACCGATCA                             <  SRR19973070.1095959/150‑1 (MQ=60)
                                                                                                                          CAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTGGAATGTTTTTCGCCGGATCTTCGGACTCGCCTGCAGCAATCCCGATCAG                            >  SRR19973070.196932/1‑150 (MQ=60)
                                                                                                                           AGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTGGAATGTTTTTCGCCGGATCTTCGGACTCGCCTGCAGCAATACCGATCAGC                           >  SRR19973070.2053293/1‑150 (MQ=60)
                                                                                                                             CAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTGGAATGTTTTTCGCCGGATCTTCGGACTCGCCTGCAGCAATACCGATCAGCTC                         >  SRR19973070.2916642/1‑150 (MQ=60)
                                                                                                                             CAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTGGAATGTTTTTCGCCGGATCTTCGGACTCGCCTGCAGCAATACCGATCAGCTC                         >  SRR19973070.2916645/1‑150 (MQ=60)
                                                                                                                              AGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTGGAATGTTTTTCGCCGGATCTTCGGACTCGCCTGCAGCAATACCGATCAGCTCG                        >  SRR19973070.2431393/1‑150 (MQ=60)
                                                                                                                              AGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTGGAATGTTTTTCGCCGGATCTTCGGACTCGCCTGCAGCAATACCGATCAGCTCG                        >  SRR19973070.2431365/1‑150 (MQ=60)
                                                                                                                                     GATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTGGAATGTTTTTCGCCGGATCTTCGGACTCGCCTGCAGCAATACCGATCAGCTCGGTTCCCT                 <  SRR19973070.1169567/150‑1 (MQ=60)
                                                                                                                                     GATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTGGAATGTTTTTCGCCGGATCTTCGGACTCGCCTGCAGCAATACCGATCAGCTCGGTTCCCT                 <  SRR19973070.1169554/150‑1 (MQ=60)
                                                                                                                                      ATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTGGAATGTTTTTCGCCGGATCTTCGGACTCGCCTGCAGCAATACCGATCAGCTCGGTTCCCTG                <  SRR19973070.83634/150‑1 (MQ=60)
                                                                                                                                       TCGGTGTACGGAATAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTGGAATGTTTTTCGCCGGATCTTCGGACTCGCCTGCAGCAATACCGATCAGCTCGGTTCCCTGG               <  SRR19973070.965132/150‑1 (MQ=60)
                                                                                                                                       TCGGTGTACGGAATAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTGGAATGTTTTTCGCCGGATCTTCGGACTCGCCTGCAGCAATACCGATCAGCTCGGTTCCCTGG               <  SRR19973070.964996/150‑1 (MQ=60)
                                                                                                                                        CGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTGGAATGTTTTTCGCCGGATCTTCGGACTCGCCTGCAGCAATACCGATCAGCTCGGTTCCCTGGA              >  SRR19973070.177421/1‑150 (MQ=60)
                                                                                                                                        CGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTGGAATGTTTTTCGCCGGATCTTCGGACTCGCCTGCAGCAATACCGATCAGCTCGGTTCCCTGGA              >  SRR19973070.2664013/1‑150 (MQ=60)
                                                                                                                                           TGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTGGAATGTTTTTCGCCGGATCTTCGGACTCGCCTGCAGCAATACCGATCAGCTCGGTTCCCTGGAAAG           <  SRR19973070.1775768/150‑1 (MQ=60)
                                                                                                                                           TGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTGGAATGTTTTTCGCCGGATCTTCGGACTCGCCTGCAGCAATACCGATCAGCTCGGTTCCCTGGAAAG           >  SRR19973070.1142132/1‑150 (MQ=60)
                                                                                                                                                GGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTGGAATGTTTTTCGCCGGATCTTCGGACTCGCCTGCAGCAATACCGATCAGCTCGGTTCCCTGGAAAGAGAAG      >  SRR19973070.1425950/1‑150 (MQ=60)
                                                                                                                                                  AAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTGGAATGTTTTTCGCCGGATCTTCGGACTCGCCTGCAGCATTACCGATCAGCTCGGTTCCCTGGAAAGAGAAGCC    >  SRR19973070.2785402/1‑150 (MQ=60)
                                                                                                                                                  AAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTGGAATGTTTTTCGCCGGATCTTCGGACTCGCCTGCAGCAATACCGATCAGCTCGGTTCCCTGGAAAGAGAAGCC    <  SRR19973070.2933522/150‑1 (MQ=60)
                                                                                                                                                   AGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTGGAATGTTTTTCGCCGGATCTTCGGACTCGCCTGCAGCAATACCGATCAGCTCGGTTCCCTGGAAAGAGAAGCCG   <  SRR19973070.3105469/150‑1 (MQ=60)
                                                                                                                                                    TAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTGGAATGTTTTTCGCCGGATCTTCGGACTCGCCTGCAGCAATACCGATCAGCTCGGTTCCCTGGAAAGAGAAGCCGA  >  SRR19973070.1098752/1‑150 (MQ=60)
                                                                                                                                                    GAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTGGAATGTTTTTCGCCGGATCTTCGGACTCGCCTGCAGCAATACCGATCAGCTCGGTTCCCTGGAAAGAGAAGCCGA  <  SRR19973070.1920054/150‑1 (MQ=60)
                                                                                                                                                    |                                                                                                                                                     
TTACCCGCTGACAGCACCGCCGTCAGAATAACTGCGTTCATCACCGCCGCCGCAGAGAGCAGACCCGCGTGCTGGAACACCAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAATAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTGGAATGTTTTTCGCCGGATCTTCGGACTCGCCTGCAGCAATACCGATCAGCTCGGTTCCCTGGAAAGAGAAGCCGA  >  CP009273/2241074‑2241371

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑