Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F309 I0 R1 1697 197.2 6627756 95.7% 6342762 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,182,647 G→T 92.1% pseudogene (1868/2445 nt) yqiG → pseudogene; fimbrial export usher family;putative membrane; Not classified; putative membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,182,6470GT92.1% 587.4 / 42.3 216pseudogene (1868/2445 nt)yqiGpseudogene; fimbrial export usher family;putative membrane; Not classified; putative membrane protein
Reads supporting (aligned to +/- strand):  ref base G (7/10);  new base T (106/93);  total (113/103)
Fisher's exact test for biased strand distribution p-value = 4.49e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

Only 100 of 220 total aligned reads displayed.

ATGACTCTTCCGATCGAAATAATTCCTGGAGCGTTTCTGCTTCAGGCGACAATGATGAATTCAAAGATATGAAAGCGTCACTACGCGCCAGTTATCAGCATAATACCGAGAACGGTCGACTCTACCTCTCCGGTACATCACAGCGAGACAGTTATTATTCTCTGAATGCCAGTTGGAATGGTTCATTCACTGCGACTCGCCACGGTGCCGCTTTCCACGACTATAGCGGTAGTGCTGACTCGCGTTTTATGATCGACGCAGACGGCACTGAAGATATTCCGTTGAACAAT  >  CP009273/3182503‑3182792
                                                                                                                                                |                                                                                                                                                 
aTGACTCTTCCGATCGAAATAATTCCTGGAGCGTTTCTGCTTCAGGCGACAATGATGAATTCAAAGATATGAAAGCGTCACTACGCGCCAGTTATCAGCATAATACCGAGAACGGTCGACTCTACCTCTCCGGTACATCACAGCtaga                                                                                                                                                >  1:2946733/1‑148 (MQ=255)
  gACTCTTCCGATCGAAATAATTCCTGGAGCGTTTCTGCTTCAGGCGACAATGATGAATTCAAAGATATGAAAGCGTCACTACGCGCCAGTTATCAGCATAATACCGAGAACGGTCGACTCTACCTCTCCGGTACATCACAGCTAGACa                                                                                                                                              <  1:669569/148‑1 (MQ=255)
   aCTCTTCCGATCGAAATAATTCCTGGAGCGTTTCTGCTTCAGGCGACAATGATGAATTCAAAGATATGAAAGCGTCACTACGCGCCAGTTATCAGCATAATACCGAGAACGGTCGACTCTACCTCTCCGGTACATCACAGCTAGACAg                                                                                                                                             >  1:200453/1‑148 (MQ=255)
   aCTCTTCCGATCGAAATAATTCCTGGAGCGTTTCTGCTTCAGGCGACAATGATGAATTCAAAGATATGAAAGCGTCACTACGCGCCAGTTATCAGCATAATACCGAGAACGGTCGACTCTACCTCTCCGGTACATCACAGCTAGACAg                                                                                                                                             <  2:1630407/148‑1 (MQ=255)
    ctctTCCGATCGAAATAATTCCTGGAGCGTTTCTGCTTCAGGCGACAATGATGAATTCAAAGATATGAAAGCGTCACTACGCGCCAGTTATCAGCATAATACCGAGAACGGTCGACTCTACCTCTCCGGTACATCACAGCTAGACAGt                                                                                                                                            >  1:1819497/1‑148 (MQ=255)
    ctctTCCGATCGAAATAATTCCTGGAGCGTTTCTGCTTCAGGCGACAATGATGAATTCAAAGATATGAAAGCGTCACTACGCGCCAGTTATCAGCATAATACCGAGAACGGTCGACTCTACCTCTCCGGTACATCACAGCTAGACAGt                                                                                                                                            <  2:814592/148‑1 (MQ=255)
    ctctTCCGATCGAAATAATTCCTGGAGCGTTTCTGCTTCAGGCGACAATGATGAATTCAAAGATATGAAAGCGTCACTACGCGCCAGTTATCAGCATAATACCGAGAACGGTCGACTCTACCTCTCCGGTACATCACAGCGAGACAGt                                                                                                                                            >  2:329300/1‑148 (MQ=255)
     tctTCCGATCGAAATAATTCCTGGAGCGTTTCTGCTTCAGGCGACAATGATGAATTCAAAGATATGAAAGCGTCACTACGCGCCAGTTATCAGCATAATACCGAGAACGGTCGACTCTACCTCTCCGGTACATCACAGCTAGACAGtt                                                                                                                                           <  2:916486/148‑1 (MQ=255)
     tctTCCGATCGAAATAATTCCTGGAGCGTTTCTGCTTCAGGCGACAATGATGAATTCAAAGATATGAAAGCGTCACTACGCGCCAGTTATCAGCATAATACCGAGAACGGTCGACTCTACCTCTCCGGTACATCACAGCTAGACAGtt                                                                                                                                           >  1:983824/1‑148 (MQ=255)
     tctTCCGATCGAAATAATTCCTGGAGCGTTTCTGCTTCAGGCGACAATGATGAATTCAAAGATATGAAAGCGTCACTACGCGCCAGTTATCAGCATAATACCGAGAACGGTCGACTCTACCTCTCCGGTACATCACAGCTAGACAGtt                                                                                                                                           <  2:1616978/148‑1 (MQ=255)
     tctTCCGATCGAAATAATTCCTGGAGCGTTTCTGCTTCAGGCGACAATGATGAATTCAAAGATATGAAAGCGTCACTACGCGCCAGTTATCAGCATAATACCGAGAACGGTCGACTCTACCTCTCCGGTACATCACAGCGAGACAGtt                                                                                                                                           <  1:1643845/148‑1 (MQ=255)
      gttCCGATCGAAATAATTCGTGGAGCGTTTCTGTTTCTGGGGACAATGATGAATTCAAAGTTTTGCAAGCGTCACTACGCGCCAGTTATCAGCTTAATACCGAGAACGGTCGACTCTAGCTCTCCGGTACATCACAGCTAGACAGtta                                                                                                                                          <  1:918578/147‑1 (MQ=255)
         ccGATCGAAATAATTCCTGGAGCGTTTCTGCTTCAGGCGACAATGATGAATTCAAAGATATGAAAGCGTCACTACGCGCCAGTTATCAGCATAATACCGAGAACGGTCGACTCTACCTCTCCGGTACATCACAGCTAGACAGttatta                                                                                                                                       <  1:2173959/148‑1 (MQ=255)
          cGATCGAAATAATTCCTGGAGCGTTTCTGCTTCAGGCGACAATGATGAATTCAAAGATATGAAAGCGTCACTACGCGCCAGTTATCAGCATAATACCGAGAACGGTCGACTCTACCTCTCCGGTACATCACAGCTAGACAGTtattat                                                                                                                                      <  1:1680739/148‑1 (MQ=255)
             tCGAAATAATTCCTGGAGCGTTTCTGCTTCAGGCGACAATGATGAATTCAAAGATATGAAAGCGTCACTACGCGCCAGTTATCAGCATAATACCGAGAACGGTCGACTCTACCTCTCCGGTACATCACAGCTAGACAGTTATTATtct                                                                                                                                   >  2:848750/1‑148 (MQ=255)
             tCGAAATAATTCCTGGAGCGTTTCTGCTTCAGGCGACAATGATGAATTCAAAGATATGAAAGCGTCACTACGCGCCAGTTATCAGCATAATACCGAGAACGGTCGACTCTACCTCTCCGGTACATCACAGCTAGACAGTTATTATtct                                                                                                                                   >  1:1130624/1‑148 (MQ=255)
              cGAAATAATTCCTGGAGCGTTTCTGCTTCAGGCGACAATGATGAATTCAAAGATATGAAAGCGTCACTACGCGCCAGTTATCAGCATAATACCGAGAACGGTCGACTCTACCTCTCCGGTACATCACAGCTAGACAGTTATTATtctc                                                                                                                                  <  2:49332/148‑1 (MQ=255)
              cGAAATAATTCCTGGAGCGTTTCTGCTTCAGGCGACAATGATGAATTCAAAGATATGAAAGCGTCACTACGCGCCAGTTATCAGCATAATACCGAGAACGGTCGACTCTACCTCTCCGGTACATCACAGCTAGACAGTTATTATtctc                                                                                                                                  >  2:2551298/1‑148 (MQ=255)
                 aataatTCCTGGAGCGTTTCTGCTTCAGGCGACAATGATGAATTCAAAGATATGAAAGCGTCACTACGCGCCAGTTATCAGCATAATACCGAGAACGGTCGACTCTACCTCTCCGGTACATCACAGCTAGACAGTTATTATTCTCTGa                                                                                                                               <  1:85574/148‑1 (MQ=255)
                  ataatTCCTGGAGCGTTTCTGCTTCAGGCGACAATTATGAATTCAAATATATGAAAGCGTCACTACGCGCCAGTTATCAGCATAATACCGAGAACGGTCGACTCTACCTCTCCGGTACATCACAGCTAGACAGTTATTATTCTCTGaa                                                                                                                              <  1:3294074/148‑1 (MQ=255)
                  ataatTCCTGGAGCGTTTCTGCTTCAGGCGACAATGATGAATTCAAAGATATGAAAGCGTCACTACGCGCCAGTTATCAGCATAATACCGAGAACGGTCGACTCTACCTCTCCGGTACATCACAGCTAGACAGTTATTATTCTCTGaa                                                                                                                              >  2:2398466/1‑148 (MQ=255)
                  ataatTCCTGGAGCGTTTCTGCTTCAGGCGACAATGATGAATTCAAAGATATGAAAGCGTCACTACGCGCCAGTTATCAGCATAATACCGAGAACGGTCGACTCTACCTCTCCGGTACATCACAGCGAGACAGTTATTATTCTCTGaa                                                                                                                              <  1:1878346/148‑1 (MQ=255)
                      ttCCTGGAGCGTTTCTGCTTCAGGCGACAATGATGAATTCAAAGATATGAAAGCGTCACTACGCGCCAGTTATCAGCATAATACCGAGAACGGTCGACTCTACCTCTCCGGTACATCACAGCTAGACAGTTATTATTCTCTGAATGcc                                                                                                                          <  2:2449633/148‑1 (MQ=255)
                         cTGGAGCGTTTCTGCTTCAGGCGACAATGATGAATTCAAAGATATGAAAGCGTCACTACGCGCCAGTTATCAGCATAATACCGAGAACGGTCGACTCTACCTCTCCGGTACATCACAGCTAGACAGTTATTATTCTCTGAATGCCAGt                                                                                                                       <  1:1333319/148‑1 (MQ=255)
                            gAGCGTTTCTGCTTCAGGCGACAATGATGAATTCAAAGATATGAAAGCGTCACTACGCGCCAGTTATCAGCATAATACCGAGAACGGTCGACTCTACCTCTCCGGTACATCACAGCTAGACAGTTATTATTCTCTGAATGCCAGTTgg                                                                                                                    >  2:3228332/1‑148 (MQ=255)
                             aGCGTTTCTGCTTCAGGCGACAATGATGAATTCAAAGATATGAAAGCGTCACTACGCGCCAGTTATCAGCATAATACCGAGAACGGTCGACTCTACCTCTCCGGTACATCACAGCTAGACAGTTATTATTCTCTGAATGCCAGTTGGa                                                                                                                   <  2:1043358/148‑1 (MQ=255)
                              gCGTTTCTGCTTCAGGCGACAATGATGAATTCAAAGATATGAAAGCGTCACTACGCGCCAGTTATCAGCATAATACCGAGAACGGTCGACTCTACCTCTCCGGTACATCACAGCTAGACAGTTATTATTCTCTGAATGCCAGTTGGaa                                                                                                                  <  2:2219681/148‑1 (MQ=255)
                               cGTTTCTGCTTCAGGCGACAATGATGAATTCAAAGATATGAAAGCGTCACTACGCGCCAGTTATCAGCATAATACCGAGAACGGTCGACTCTACCTCTCCGGTACATCACAGCTAGACAGTTATTATTCTCTGAATGCCAGGTGGAAt                                                                                                                 >  2:3156940/1‑148 (MQ=255)
                                     tGCTTCAGGCGACAATGATGAATTCAAAGATATGAAAGCGTCACTACGCGCCAGTTATCAGCATAATACCGAGAACGGTCGACTCTACCTCTCCGGTACATCACAGCTAGACAGTTATTATTCTCTGAATGCCAGTTGGAATGGttca                                                                                                           >  1:2550596/1‑148 (MQ=255)
                                      gCTTCAGGCGACAATGATGAATTCAAAGATATGAAAGCGTCACTACGCGCCAGTTATCAGCATAATACCGAGAACGGTCGACTCTACCTCTCCGGTACATCACAGCTAGACAGTTATTATTCTCTGAATGCCAGTTGGAATGGttcat                                                                                                          >  2:2770966/1‑148 (MQ=255)
                                        ttCAGGCGACAATGCTGAATTCAAAGATATGAAAGCGTCACTACGCGCCAGTTATCAGCATAATACCGAGAACGGTCGACTCTACCTCTCCGGTACATCACAGCTAGACAGTTATTATTCTCTGAATGCCAGTTGGAATGGttcattc                                                                                                        <  2:302490/148‑1 (MQ=255)
                                         tCAGGCGACAATGATGAATTCAAAGATATGAAAGCGTCACTACGCGCCAGTTATCAGCATAATACCGAGAACGGTCGACTCTACCTCTCCGGTACATCACAGCTAGACAGTTATTATTCTCTGAATGCCAGTTGGAATGGttcattca                                                                                                       <  1:2669300/148‑1 (MQ=255)
                                                aCAATGATGAATTCAAAGATATGAAAGCGTCACTACGCGCCAGTTATCAGCATAATACCGAGAACGGTCGACTCTACCTCTCCGGTACATCACAGCTAGACAGTTATTATTCTCTGAATGCCAGTTGGAATGGTTCATTCACTGCGAc                                                                                                >  2:1904272/1‑148 (MQ=255)
                                                aCAATGATGAATTCAAAGATATGAAAGCGTCACTACGCGCCAGTTATCAGCATAATACCGAGAACGGTCGACTCTACCTCTCCGGTACATCACAGCTAGACAGTTATTATTCTCTGAATGCCAGTTGGAATGGTTCATTCACTGCGAc                                                                                                >  2:612016/1‑148 (MQ=255)
                                                aCAATGATGAATTCAAAGATATGAAAGCGTCACTACGCGCCAGTTATCAGCATAATACCGAGAACGGTCGACTCTACCTCTCCGGTACATCACAGCGAGACAGTTATTATTCTCTGAATGCCAGTTGGAATGGTTCATTCACTGCGAc                                                                                                >  1:2922814/1‑148 (MQ=255)
                                                 cAATGATGAATTCAAAGATATGAAAGCGTCACTACGCGCCAGTTATCAGCATAATACCGAGAACGGTCGACTCTACCTCTCCGGTACATCACAGCGAGACAGTTATTATTCTCTGAATGCCAGTTGGAATGGTTCATTCACTGCGACt                                                                                               >  2:2944720/1‑148 (MQ=255)
                                                  aaTGATGAATTCAAAGATATGAAAGCGTCACTACGCGCCAGTTATCAGCATAATACCGAGAACGGTCGACTCTACCTCTCCGGTACATCACAGCTAGACAGTTATTATTCTCTGAATGCCAGTTGGAATGGTTCATTCACTGCGACTc                                                                                              >  1:1899849/1‑148 (MQ=255)
                                                   atgatgAATTCAAAGATATGAAAGCGTCACTACGCGCCAGTTATCAGCATAATACCGAGAACGGTCGACTCTACCTCTCCGGTACATCACAGCTAGACAGTTATTATTCTCTGAATGCCAGTTGGAATGGTTCATTCACTGCGACTCg                                                                                             <  2:172466/148‑1 (MQ=255)
                                                      atgaATTCAAAGATATGAAAGCGTCACTACGCGCCAGTTATCAGCATAATACCGAGAACGGTCGACTCTACCTCTCCGGTACATCACAGCTAGACAGTTATTATTCTCTGAATGCCAGTTGGAATGGTTCATTCACTGCGACTCGCCa                                                                                          >  2:1108280/1‑148 (MQ=255)
                                                        gaATTCAAAGATATGAAAGCGTCACTACGCGCCAGTTATCAGCATAATACCGAGAACGGTCGACTCTACCTCTCCGGTACATCACAGCTAGACAGTTATTATTCTCTGAATGCCAGTTGGAATGGTTCATTCACTGCGACTCGCCACg                                                                                        >  1:2233532/1‑148 (MQ=255)
                                                            tCAAAGATATGAAAGCGTCACTACGCGCCAGTTATCAGCATAATACCGAGAACGGTCGACTCTACCTCTCCGGTACATCACAGCTAGACAGTTATTATTCTCTGAATGCCAGTTGGAATGGTTCATTCACTGCGACTCGCCACGGTGc                                                                                    >  2:1325642/1‑148 (MQ=255)
                                                            tCAAAGATATGAAAGCGTCACTACGCGCCAGTTATCAGCATAATACCGAGAACGGTCGACTCTACCTCTCCGGTACATCACAGCTAGACAGTTATTATTCTCTGAATGCCAGTTGGAATGGTTCATTCACTGCGACTCGCCACGGTGc                                                                                    >  1:1008498/1‑148 (MQ=255)
                                                             cAAAGATATGAAAGCGTCACTACGCGCCAGTTATCAGCATAATACCGAGAACGGTCGACTCTACCTCTCCGGTACATCACAGCTAGACAGTTATTATTCTCTGAATGCCAGTTGGAATGGTTCATTCACTGCGACTCGCCACGGTGcc                                                                                   >  1:1533219/1‑148 (MQ=255)
                                                                   tatGAAAGCGTCACTACGCGCCAGTTATCAGCATAATACCGAGAACGGTCGACTCTACCTCTCCGGTACATCACAGCTAGACAGTTATTATTCTCTGAATGCCAGTTGGAATGGTTCATTCACTGCGACTCGCCACGGTGCCGCTTTc                                                                             <  2:2300163/148‑1 (MQ=255)
                                                                    atGAAAGCGTCACTACGCGCCAGTTATCAGCATAATACCGAGAACGGTCGACTCTACCTCTCCGGTACATCACAGCTAGACAGTTATTATTCTCTGAATGCCAGTTGGAATGGTTCATTCACTGCGACTCGCCACGGTGCCGCTTTcc                                                                            >  1:692024/1‑148 (MQ=255)
                                                                    atGAAAGCGTCACTACGCGCCAGTTATCAGCATAATACCGAGAACGGTCGACTCTACCTCTCCGGTACATCACAGCTAGACAGTTATTATTCTCTGAATGCCAGTTGGAATGGTTCATTCACTGCGACTCGCCACGGTGCCGCTTTcc                                                                            >  1:1271519/1‑148 (MQ=255)
                                                                    atGAAAGCGCCACTACGCGCCAGTTATCAGCATAATACCGAGAACGGTCGACTCTACCTCTCCGGTACATCACAGCTAGACAGTTATTATTCTCTGAATGCCAGTTGGAATGGTTCATTCACTGCGACTCGCCACGGTGCCGCTTTcc                                                                            >  1:2764702/1‑148 (MQ=255)
                                                                      gAAAGCGTCACTACGCGCCAGTTATCAGCATAATACCGAGAACGGTCGACTCTACCTCTCCGGTACATCACAGCTAGACAGTTATTATTCTCTGAATGCCAGTTGGAATGGTTCATTCACTGCGACTCGCCACGGTGCCGCTTTCCAc                                                                          >  1:517918/1‑148 (MQ=255)
                                                                       aaaGCGTCACTACGCGCCAGTTATCAGCATAATACCGAGAACGGTCGACTCTACCTCTCCGGTACATCACAGCTAGACAGTTATTATTCTCTGAATGCCAGTTGGAATGGTTCATTCACTGCGACTCGACACGGTGCCGCTTTCCACg                                                                         >  2:3267197/1‑148 (MQ=255)
                                                                       aaaGCGTCACTACGCGCCAGTTATCAGCAAAATACCGAGAACGGTCGACTCTACCTCTCCGGTACATCACAGCTAGACAGTTATTATTCTCTGAATGCCAGTTGGAATGGTTCATTCACTGCGACTCGCCACGGTGCCGCTTTCCACg                                                                         >  1:2813946/1‑148 (MQ=255)
                                                                          gCGTCACTACGCGCCAGTTATCAGCATAATACCGAGAACGGTCGACTCTACCTCTCCGGTACATCACAGCTAGACAGTTATTATTCTCTGAATGCCAGTTGGAAGGGTTCATTCACTGCGACTCGCCACGGTGCCGCTTTCCACGACt                                                                      >  2:865525/1‑148 (MQ=255)
                                                                           cGTCACTACGCGCCAGTTATCAGCATAATACCGAGAACGGTCGACTCTACCTCTCCGGTACATCACAGCTAGACAGTTATTATTCTCTGAATGCCAGTTGGAATGGTTCATTCACTGCGACTCGCCACGGTGCCGCTTTCCACGACta                                                                     >  1:2413966/1‑148 (MQ=255)
                                                                             tCACTACGCGCCAGTTATCAGCATAATACCGAGAACGGTCGACTCTACCTCTCCGGTACATCACAGCTAGACAGTTATTATTCTCTGAATGCCAGTTGGAATGGTTCATTCACTGCGACTCGCCACGGTGCCGCTTTCCACGACtata                                                                   >  1:3024706/1‑148 (MQ=255)
                                                                               aCTACGCGCCAGTTATCAGCATAATACCGAGAACGGTCGACTCTACATCTCCGGTACATCACAGCTAGACAGTTATTATTCTCTGAATGCCAGTTGGAATGGTTCATTCACTGCGACTCGCCACGGTGCCGCTTTCCACGACTATAGc                                                                 >  1:2880415/1‑148 (MQ=255)
                                                                                 tACGCGCCAGTTATCAGCATAATACCGAGAACGGTCGACTCTACCTCTCCGGTACATCACAGCTAGACAGTTATTATTCTCTGAATGCCAGTTGGAATGGTTCATTCACTGCGACTCGCCACGGTGCCGCTTTCCACGACTATAGCgg                                                               <  1:752778/148‑1 (MQ=255)
                                                                                    gcgcCAGTTATCAGCATAATACCGAGAACGGTCGACTCTACCTCTCCGGTACATCACAGCTAGACAGTTATTATTCTCTGAATGCCAGTTGGAATGGTTCATTCACTGCGACTCGCCACGGTGCCGCTTTCCACGACTATAGCGGTAg                                                            <  1:2637180/148‑1 (MQ=255)
                                                                                    gcgcCAGTTATCAGCATAATACCGAGAACGGTCGACTCTACCTCTCCGGTACATCACAGCTAGACAGTTATTATTCTCTGAATGCCAGTTGGAATGGTTCATTCACTGCGACTCGCCACGGTGCCGCTTTCCACGACTATAGCGGTAg                                                            <  1:540264/148‑1 (MQ=255)
                                                                                      gcCAGTTATCAGCATAATACCGAGAACGGTCGACTCTACCTCTCCGGTACATCACAGCTAGACAGTTATTATTCTCTGAATGCCAGTTGGAATGGTTCATTCACTGCGACTCGCCACGGTGCCGCTTTCCACGACTATAGCGGTAGTg                                                          <  2:2957661/148‑1 (MQ=255)
                                                                                       ccAGTTATCAGCATAATACCGAGAACGGTCGACTCTACCTCTCCGGTACATCACAGCTAGACAGTTATTATTCTCTGAATGCCAGTTGGAATGGTTCATTCACTGCGACTCGCCACGGTGCCGCTTTCCACGACTATAGCGGTAGTGc                                                         >  1:2181697/1‑148 (MQ=255)
                                                                                           ttATCAGCATAATACCGAGAACGGTCGACTCTACCTCTCCGGTACATCACAGCTAGACAGTTATTATTCTCTGAATGCCAGTTGGAATGGTTCATTCACTGCGACTCGCCACGGTGCCGCTTTCCACGACTATAGCGGTAGTGCTGAc                                                     >  1:1247010/1‑148 (MQ=255)
                                                                                            tATCAGCATAATACCGAGAACGGTCGACTCTACCTCTCCGGTACATCACAGCTAGACAGTTATTATTCTCTGAATGCCAGTTGGAATGGTTCATTCACTGCGACTCGCCACGGTGCCGCTTTCCACGACTATAGCGGTAGTGCTGACt                                                    <  2:1449952/148‑1 (MQ=255)
                                                                                              tCAGCATAATACCGAGAACGGTCGACTCTACCTCTCCGGTACATCACAGCTAGACAGTTATTATTCTCTGAATGCCAGTTGGAATGGTTCATTCACTGCGACTCGCCACGGTGCCGCTTTCCACGACTATAGCGGTAGTGCTGACTcg                                                  <  2:892320/148‑1 (MQ=255)
                                                                                                 gCATAATACCGAGAACGGTCGACTCTACCTCTCCGGTACATCACAGCTAGACAGTTATTATTCTCTGAATGCCAGTTGGAATGGTTCATTCACTGCGACTCGCCACGGTGCCGCTTTCCACGACTATAGCGGTAGTGCTGACTCGCGt                                               >  1:3139533/1‑148 (MQ=255)
                                                                                                  cATAATACCGAGAACGGTCGACTCTACCTCTCCGGTACATCACAGCTAGACAGTTATTATTCTCTGAATGCCAGTTGGAATGGTTCATTCACTGCGACTCGCCACGGTGCCGCTTTCCACGACTATAGCGGTAGTGCTGACTCGCGtt                                              >  2:2113509/1‑148 (MQ=255)
                                                                                                   ataataCCGAGAACGGTCGACTCTACCTCTCCGGTACATCACAGCTAGACAGTTATTATTCTCTGAATGCCAGTTGGAATGGTTCATTCACTGCGACTCGCCACGGTGCCGCTTTCCACGACTATAGCGGTAGTGCTGACTCGCGttt                                             >  2:1952832/1‑148 (MQ=255)
                                                                                                    taataCCGAGACCGGTCGACTCTACCTCTCCGGTACATCACAGCTAGACAGTTATTATTCTCTGAATGCCAGTTGGAATGGTTCATTCACTGCGACTCGCCACGGTGCCGCTTTCCACGACTATAGCGGTAGTGCTGACTCGCGtttt                                            <  2:2838742/148‑1 (MQ=255)
                                                                                                    taataCCGAGAACGGTCGACTCTACCTCTCCGGTACATCACAGCTAGACAGTTATTATTCTCTGAATGCCAGTTGGAATGGTTCATTCACTGCGACTCGCCACGGTGCCGCTTTCCACGACTATAGCGGTAGTGCTGACTCGCGtttt                                            >  2:97243/1‑148 (MQ=255)
                                                                                                     aataCCGAGAACGGTCGACTCTACCTCTCCGGTACATCACAGCTAGACAGTTATTATTCTCTGAATGCCAGTTGGAATGGTTCATTCACTGCGACTCGCCACGGTGCCGCTTTCCACGACTATAGCGGTAGTGCTGACTCGCGTTTTa                                           >  1:1907259/1‑148 (MQ=255)
                                                                                                     aataCCGAGAACGGTCGACTCTACCTCTCCGGTACATCACAGCTAGACAGTTATTATTCTCTGAATGCCAGTTGGAATGGTTCATTCACTGCGACTCGCCACGGTGCCGCTTTCCACGACTATAGCGGTAGTGCTGACTCGCGTTTTa                                           <  1:2499773/148‑1 (MQ=255)
                                                                                                     aataCCGAGAACGGTCGACTCTACCTCTCCGGTACATCACAGCTAGACAGTTATTATTCTCTGAATGCCAGTTGGAATGGTTCATTCACTGCGACTCGCCACGGTGCCGCTTTCCACGACTATAGCGGTAGTGCTGACTCGCGTTTTa                                           <  1:1108280/148‑1 (MQ=255)
                                                                                                       taCCGAGAACGGTCGACTCTACCTCTCCGGTACATCACAGCTAGACAGTTATTATTCTCTGAATGCCAGTTGGAATGGTTCATTCACTGCGACTCGCCACGGTGCCGCTTTCCACGACTATAGCGGTAGTGCTGACTCGCGTTTTATg                                         >  2:2004242/1‑148 (MQ=255)
                                                                                                       taCCGAGAACGGTCGACTCTACCTCTCCGGTACATCACAGCTAGACAGTTATTATTCTCTGAATGCCAGTTGGAATGGTTCATTCACTGCGACTCGCCACGGTGCCGCTTTCCACGACTATAGCGGTAGTGCTGACTCGCGTTTTATg                                         >  1:3079169/1‑148 (MQ=255)
                                                                                                           gagaACGGTCGACTCTACCTCTCCGGTACATCACAGCTAGACAGTTATTATTCTCTGAATGCCAGTTGGAATGGTTCATTCACTGCGACTCGCCACGGTGCCGCTTTCCACGACTATAGCGGTAGTGCTGACTCGCGTTTTATGATCg                                     <  2:1819497/148‑1 (MQ=255)
                                                                                                            agaAGGGTCGTCTCTACCTATCCGGTACATCACAGCTAGACAGTTATTATTCTCTGAATGCCAGTTGGAATGGTTAATTCACTGCGACTCGCCTCGGTGCGGCTTTCCACGAATATAGCGGTAGTGCTGACTCGCGTTTTATGATCGa                                    <  2:2813946/148‑1 (MQ=255)
                                                                                                            agaACGGCCGACTCTACCTCTCCGGTACATCACAGCTAGACAGTTATTATTCTCTGAATGCCAGTTGGAATGGTTCATTCACTGCGACTCGCCACGGTGCCGCTTTCCACGACTATAGCGGTAGTGCTGACTCGCGTTTTATGATCGa                                    <  2:1548711/148‑1 (MQ=255)
                                                                                                             gaACGGTCGACTCTACCTCTCCGGTACATCACAGCTAGACAGTTATTATTCTCTGAATGCCAGTTGGAATGGTTCATTCACTGCGACTCGCCACGGTGCCGCTTTCCACGACTATAGCGGTAGTGCTGACTCGCGTTTTATGATCGAc                                   >  1:2102020/1‑148 (MQ=255)
                                                                                                                     gACTCTACCTCTCCGGTACATCACAGCTAGACAGTTATTATTCTCTGAATGCCAGTTGGAATGGTTCATTCACTGCGACTCGCCACGGTGCCGCTTTCCACGACTATAGCGGTAGTGCTGACTCGCGTTTTATGATCGACGCAGACgg                           <  2:1130624/148‑1 (MQ=255)
                                                                                                                       ctctACCTCTCCGGTACATCACAGCTAGACAGTTATTATTCTCTGAATGCCAGTTGGAATGGTTCATTCACTGCGACTCGCCACGGTGCCGCTTTCCACGACTATAGCGGTAGTGCTGACTCGCGTTTTATGATCGACGCAGACGGCa                         >  2:506051/1‑148 (MQ=255)
                                                                                                                       ctctACCTCTCCGGTACATCACAGCTAGACAGTTATTATTCTCTGAATGCCAGTTGGAATGGTTCATTCACTGCGACTCGCCACGGTGCCGCTTTCCACGACTATAGCGGTAGTGCTGACTCGCGTTTTATGATCGACGCAGACGGCa                         <  1:1745348/148‑1 (MQ=255)
                                                                                                                         ctACCTCTCCGGTACATCACAGCTAGACAGTTATTATTCTCTGAATGCCAGTTGGAATGGTTCATTCACTGCGACTCGCCACGGTGCCGCTTTCCACGACTATAGCGGTAGTGCTGACTCGCGTTTTATGATCGACGCAGACGGCACt                       <  2:1215634/148‑1 (MQ=255)
                                                                                                                           aCCTCTCCGGTACATCACAGCTAGACAGTTATTATTCTCTGAATGCCAGTTGGAATGGTTCATTCACTGCGACTCGCCACGGTGCCGCTTTCCACGACTATAGCGGTAGTGCTGACTCGCGTTTTATGATCGACGCAGACGGCACTGa                     <  2:983823/148‑1 (MQ=255)
                                                                                                                           aCCTCTCCGGTACATCACAGCTAGACAGTTATTATTCTCTGAATGCCAGTTGGAATGGTTCATTCACTGCGACTCGCCACGGTGCCGCTTTCCACGACTATAGCGGTAGTGCTGACTCGCGTTTTATGATCGACGCAGACGGCACTGa                     <  2:1224861/148‑1 (MQ=255)
                                                                                                                           aCCTCTCCGGTACATCACAGCGAGACAGTTATTATTCTCTGAATGCCAGTTGGAATGGTTCATTCACTGCGACTCGCCACGGTGCCGCTTTCCACGACTATAGCGGTAGTGCTGACTCGCGTTTTATGATCGACGCAGACGGCACTGa                     <  1:656473/148‑1 (MQ=255)
                                                                                                                            ccTCTCCGGTACATCACAGCTAGACAGTTATTATTCTCTGAATGCCAGTTGGAATGGTTCATTCACTGCGACTCGCCACGGTGCCGCTTTCCACGACTATAGCGGTAGTGCTGACTCGCGTTTTATGATCGACGCAGACGGCACTGaa                    >  1:1076857/1‑148 (MQ=255)
                                                                                                                            ccTCTCCGGTACATCACAGCTAGACAGTTATTATTCTCTGAATGCCAGTTGGAATGGTTCATTCACTGCGACTCGCCACGGTGCCGCTTTCCACGACTATAGCGGTAGTGCTGACTCGCGTTTTATGATCGACGCAGACGGCACTGaa                    >  1:957938/1‑148 (MQ=255)
                                                                                                                               ctcCGGTACATCACAGCTAGACAGTTATTATTCTCTGAATGCCAGTTGGAATGGTTCATTCACTGCGACTCGCCACGGTGCCGCTTTCCACGACTATAGCGGTAGTGCTGACTCGCGTTTTATGATCGACGCAGACGGCACTGAAGat                 >  1:1563135/1‑148 (MQ=255)
                                                                                                                                tcCGGTACATCACAGCTAGACAGTTATTATTCTCTGAATGCCAGTTGGAATGGTTCATTCACTGCGACTCGCCACGGTGCCGCTTTCCACGACTATAGCGGTAGTGCTGACTCGCGTTTTATGATCGACGCAGACGGCACTGAAGata                <  1:1858839/148‑1 (MQ=255)
                                                                                                                                tcCGGTACATCACAGCTAGACAGTTATTATTCTCTGAATGCCAGTTGGAATGGTTCATTCACTGCGACTCGCCACGGTGCCGCTTTCCACGACTATAGCGGTAGTGCTGACTCGCGTTTTATGATCGACGCAGACGGCACTGAAGata                <  1:492515/148‑1 (MQ=255)
                                                                                                                                 ccGGTACATCACAGCTAGACAGTTATTATTCTCTGAATGCCAGTTGGAATGGTTCATTCACTGCGACTCGCCACGGTGCCGCTTTCCACGACTATAGCGGTAGTGCTGACTCGCGTTTTATGATCGACGCAGACGGCACTGAAGatat               >  2:2681747/1‑148 (MQ=255)
                                                                                                                                 ccGGTACATCACAGCTAGACAGTTATTATTCTCTGAATGCCAGTTGGAATGGTTCATTCACTGCGACTCGCCACGGTGCCGCTTTCCACGACTATAGCGGTAGTGCTGACTCGCGTTTTATGATCGACGCAGACGGCACTGAAGatat               <  1:1766900/148‑1 (MQ=255)
                                                                                                                                 ccGGTACATCACAGCTAGACAGTTATTATTCTCTGAATGCCAGTTGGAATGGTTCATTCACTGCGACTCGCCACGGTGCCGCTTTCCACGACTATAGCGGTAGTGCTGACTCGCGTTTTATGATCGACGCAGACGGCACTGAAGatat               >  1:750364/1‑148 (MQ=255)
                                                                                                                                     tACATCACAGCTAGACAGTTATTATTCTCTGAATGCCAGTTGGAATGGTTCATTCACTGCGACTCGCCACGGTGCCGCTTTCCACGACTATAGCGGTAGTGCTGACTCGCGTTTTATGATCGACGCAGACGGCACTGAAGATATTCCg           >  1:1000775/1‑148 (MQ=255)
                                                                                                                                     tACATCACAGCTAGACAGTTATTATTCTCTGAATGCCAGTTGGAATGGTTCATTCACTGCGACTCGCCACGGTGCCGCTTTCCACGACTATAGCGGTAGTGCTGACTCGCGTTTTATGATCGACGCAGACGGCACTGAAGATATTCCg           >  2:2866360/1‑148 (MQ=255)
                                                                                                                                     tACATCACAGCTAGACAGTTATTATTCTCTGAATGCCAGTTGGAATGGTTCATTCACTGCGACTCGCCACGGTGCCGCTTTCCACGACTATAGCGGTAGTGCTGACTCGCGTTTTATGATCGACGCAGACGGCACTGAAGATATTCCg           <  2:2826638/148‑1 (MQ=255)
                                                                                                                                       cATCACAGCTAGACAGTTATTATTCTCTGATTGCCAGTTGTAATGGTTCATTCACTGCGACTCGCCACGGTGCCGCTTTCCACGCCTATAGCGGTAGTGCTGACTCGCGTTTTTTGATCGACGCAGACGGCACTGAAGATATTCCGtt         <  2:3275778/148‑1 (MQ=255)
                                                                                                                                       cATCACAGCTAGACAGTTATTATTCTCTGAATGCCAGTTGGAATGGTTCATTCACTGCGACTCGCCACGGTGCCGCTTTCCACGACTATAGCGGTAGTGCTGACTCGCGTTTTATGATCGACGCAGACGGCACTGAAGATATTCCGtt         <  1:406077/148‑1 (MQ=255)
                                                                                                                                          cacaGCTAGACAGTTATTATTCTCTGAATGCCAGTTGGAATGGTTCATTCACTGCGACTCGCCACGGTGCCGCTTTCCACGACTATAGCGGTAGTGCTGACTCGCGTTTTATGATCGACGCAGACGGCACTGAAGATATTCCGTTGaa      >  2:424726/1‑148 (MQ=255)
                                                                                                                                           acaGCTAGACAGTTATTATTCTCTGAATGCCAGTTGGAATGGTTCATTCACTGCGACTCGCCACGGTGCCGCTTTCCACGACTATAGCGGTAGTGCTGACTCGCGTTTTATGATCGACGCAGACGGCACTGAAGATATTCCGTTGAAc     >  2:2389476/1‑148 (MQ=255)
                                                                                                                                             aGCTAGACAGTTATTATTCTCTGAATGCCAGTTGGAATGGTTCATTCACTGCGACTCGCCACGGTGCCGCTTTCCACGACTATAGCGGTAGTGCTGACTCGCGTTTTATGATCGACGCAGACGGCACTGAAGATATTCCGTTGAACaa   >  2:1125535/1‑148 (MQ=255)
                                                                                                                                              gCGAGACAGTTATTATTCTCTGAATGCCAGTTGGAATGGTTCATTCACTGCGACTCGCCACGGTGCCGCTTTCCACGACTATAGCGGTAGTGCTGACTCGCGTTTTATGATCGACGCAGACGGCACTGAAGATATTCCGTTGAACAAt  <  1:2202027/148‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                                 
ATGACTCTTCCGATCGAAATAATTCCTGGAGCGTTTCTGCTTCAGGCGACAATGATGAATTCAAAGATATGAAAGCGTCACTACGCGCCAGTTATCAGCATAATACCGAGAACGGTCGACTCTACCTCTCCGGTACATCACAGCGAGACAGTTATTATTCTCTGAATGCCAGTTGGAATGGTTCATTCACTGCGACTCGCCACGGTGCCGCTTTCCACGACTATAGCGGTAGTGCTGACTCGCGTTTTATGATCGACGCAGACGGCACTGAAGATATTCCGTTGAACAAT  >  CP009273/3182503‑3182792

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GGTATGACTCTTCCGATCGAAATAATTCCTGGAGCGTTTCTGCTTCAGGCGACAATGATGAATTCAAAGATATGAAAGCGTCACTACGCGCCAGTTATCAGCATAATACCGAGAACGGTCGACTCTACCTCTCCGGTACATCACAGCGAGACAGTTATTATTCTCTGAATGCCAGTTGGAATGGTTCATTCACTGCGACTCGCCACGGTGCCGCTTTCCACGACTATAGCGGTAGTGCTGACTCGCGTTTTATGATCGACGCAGACGGCACTGAAGATATTCCGTTGAACAATAA  >  CP009273/3182500‑3182794
                                                                                                                                                   |                                                                                                                                                   
GGTATGACTCTTCCGATCGAAATAATTCCTGGAGCGTTTCTGCTTCAGGCGACAATGATGAATTCAAAGATATGAAAGCGTCACTACGCGCCAGTTATCAGCATAATACCGAGAACGGTCGACTCTACCTCTCCGGTACATCACAGCTAG                                                                                                                                                   <  SRR19973070.465052/150‑1 (MQ=60)
 GTATGACTCTTCCGATCGAAATAATTCCTGGAGCGTTTCTGCTTCAGGCGACAATGATGAATTCAAAGATATGAAAGCGTCACTACGCGCCAGTTATCAGCATAATACCGAGAACGGTCGACTCTACCTCTCCGGTACATCACAGCTAGA                                                                                                                                                  >  SRR19973070.2965710/1‑150 (MQ=60)
 GTATGACTCTTCCGATCGAAATAATTCCTGGAGCGTTTCTGCTTCAGGCGACAATGATGAATTCAAAGATATGAAAGCGTCACTACGCGCCAGTTATCAGCATAATACCGAGAACGGTCGACTCTACCTCTCCGGTACATCACAGCTAGA                                                                                                                                                  >  SRR19973070.2965705/1‑150 (MQ=60)
   ATGACTCTTCCGATCGAAATAATTCCTGGAGCGTTTCTGCTTCAGGCGACAATGATGAATTCAAAGATATGAAAGCGTCACTACGCGCCAGTTATCAGCATAATACCGAGAACGGTCGACTCTACCTCTCCGGTACATCACAGCTAGACA                                                                                                                                                <  SRR19973070.1850583/150‑1 (MQ=60)
   ATGACTCTTCAGATCGAAATAATTCCTGGAGCGTTTCTGCTTCAGTCGACAATGATGAATTCAAAGATATGAAAGCGTCACTACGCGCCAGTTATCAGCATAATACCGAGAACGGTCGACTCTACCTCTCCGGTACATCACAGCTAGACA                                                                                                                                                <  SRR19973070.1850582/150‑1 (MQ=60)
    TGACTCTTCCGATCGAAATAATTCCTGGAGCGTTTCTGCTTCAGGCGACAATGATGAATTCAAAGATATGAAAGCGTCACTACGCGCCAGTTATCAGCATAATACCGAGAACGGTCGACTCTACCTCTCCGGTACATCACAGCTAGACAG                                                                                                                                               >  SRR19973070.201996/1‑150 (MQ=60)
    TGACTCTTCCGATCGAAATAATTCCTGGAGCGTTTCTGCTTCAGGCGACAATGATGAATTCAAAGATATGAAAGCGTCACTACGCGCCAGTTATCAGCATAATACCGAGAACGGTCGACTCTACCTCTCCGGTACATCACAGCTAGACAG                                                                                                                                               >  SRR19973070.201973/1‑150 (MQ=60)
     GACTCTTCCGATCGAAATAATTCCTGGAGCGTTTCTGCTTCAGGCGACAATGATGAATTCAAAGATATGAAAGCGTCACTACGCGCCAGTTATCAGCATAATACCGAGAACGGTCGACTCTACCTCTCCGGTACATCACAGCTAGACAGT                                                                                                                                              <  SRR19973070.674348/150‑1 (MQ=60)
     GACTCTTCCGATCGAAATAATTCCTGGAGCGTTTCTGCTTCAGGCGACAATGATGAATTCAAAGATATGAAAGCGTCACTACGCGCCAGTTATCAGCATAATACCGAGAACGGTCGACTCTACCTCTCCGGTACATCACAGCTAGACAGT                                                                                                                                              >  SRR19973070.1832033/1‑150 (MQ=60)
     GACTCTTCCGATCGAAATAATTCCTGGAGCGTTTCTGCTTCAGGCGACAATGATGAATTAAAAGATATGAAAGCGTCACTACGCGCCAGTTATCAGCATAATACCGAGAACGGTCGACTCTACCTCTCCGGTACATCACAGCTAGACAGT                                                                                                                                              <  SRR19973070.674450/150‑1 (MQ=60)
      ACTCTTCCGATCGAAATAATTCCTGGAGCGTTTCTGCTTCAGGCGACAATGATGAATTCAAAGATATGAAAGCGTCACTACGCGCCAGTTATCAGCATAATACCGAGAACGGTCGACTCTACCTCTCCGGTACATCACAGCTAGACAGTT                                                                                                                                             >  SRR19973070.1233433/1‑150 (MQ=60)
      ACTCTTCCGATCGAAATAATTCCTGGAGCGTTTCTGCTTCAGGCGACAATGATGAATTCAAAGATATGAAAGCGTCACTACGCGCCAGTTATCAGCATAATACCGAGAACGGTCGACTCTACCTCTCCGGTACATCACAGCTAGACAGTT                                                                                                                                             <  SRR19973070.1526474/150‑1 (MQ=60)
      ACTCTTCCGATCGAAATAATTCCTGGAGCGTTTCTGCTTCAGGCGACAATGATGAATTCAAAGATATGAAAGCGTCACTACGCGCCAGTTATCAGCATAATACCGAGAACGGTCGACTCTACCTCTCCGGTACATCACAGCTAGACAGTT                                                                                                                                             >  SRR19973070.990834/1‑150 (MQ=60)
      ACTCTTCCGATCGAAATAATTCCTGGAGCGTTTCTGCTTCAGGCGACAATGATGAATTCAAAGATATGAAAGCGTCACTACGCGCCAGTTATCAGCATAATACCGAGAACGGTCGACTCTACCTCTCCGGTACATCACAGCTAGACAGTT                                                                                                                                             >  SRR19973070.990835/1‑150 (MQ=60)
       CTCTTCCGATCGAAATAATTCCTGGAGCGTTTCTGCTTCAGGCGACAATGATGAATTCAAAGATATGAAAGCGTCACTACGCGCCAGTTATCAGCATAATACCGAGAACGGTAGACTCTACCTCTCCGGTACATCACAGCTAGACAGTTA                                                                                                                                            <  SRR19973070.2164714/150‑1 (MQ=60)
        TCTTCCGATCGAAATAATTCCTGGAGCGTTTCTGCTTCAGGCGACAATGATGAATTCAAAGATATGAAAGCGTCACTACGCGCCAGTTATCAGCATAATACCGAGAACGGTCGACTCTACCTCTCCGGTACATCACAGCGAGACAGTTAT                                                                                                                                           <  SRR19973070.1655205/150‑1 (MQ=60)
         GTTCCGATCGAAATAATTCGTGGAGCGTTTCTGTTTCTGGGGACAATGATGAATTCAAAGTTTTGCAAGCGTCACTACGCGCCAGTTATCAGCTTAATACCGAGAACGGTCGACTCTAGCTCTCCGGTACATCACAGCTAGACAGTTATT                                                                                                                                          <  SRR19973070.925121/150‑1 (MQ=60)
         CTTCCGATCGAAATAATTCCTGGAGCGTTTCTGCTTCAGGCGACAATGATGAATTCAAAGATATGAAAGCGTCACTACGCGCCAGTTATCAGCATAATACCGAGAACGGTCGACTCTACCTCTCCGGTACATCACAGCTAGACAGTTATT                                                                                                                                          <  SRR19973070.925346/150‑1 (MQ=60)
            CCGATCGAAATAATTCCTGGAGCGTTTCTGCTTCAGGCGACAATGATGAATTCAAAGATATGAAAGCGTCACTACGCGCCAGTTATCAGCATAATACCGAGAACGGTCGACTCTACCTCTCCGGTACATCACAGCTAGACAGTTATTATT                                                                                                                                       <  SRR19973070.2188368/150‑1 (MQ=60)
            CCGATCGAAATAATTCCTGGAGCGTTTCTGCTTCAGGCGACAATGATGAATTCAAAGATATGAAAGCGTCACTACGCGCCAGTTATCAGCATAATACCGAGAACGGTCGACTCTACCTCTCCGGTACATCACAGCTAGACAGTTATTATT                                                                                                                                       <  SRR19973070.2188526/150‑1 (MQ=60)
             CGATCTAAATAATTCCTGGAGCGTTTCTGCTTCAGGCGACAATGATGAATTCAAAGATATGAAAGCGTCACTACNCGCCAGTTATCAGCATAATACCGAGAACGGTCGACTCTACCTCTCCGGTACATCACAGCTAGACAGTTATTATTC                                                                                                                                      <  SRR19973070.1692251/150‑1 (MQ=60)
              GATCGAAATAATTCCTGGAGCGTTTCTGCTTCAGGCGACAATGATGAATTCAAAGATATGAAAGCGTCACTACGCGCCAGTTATCAGCATAATACCGAGAACGGTCGACTCTACCTCTCCGGTACATCACAGCTAGACAGTTATTATTCT                                                                                                                                     >  SRR19973070.1137638/1‑150 (MQ=60)
              GATCGAAATAATTCCTGGAGCGTTTCTGCTTCAGGCGACAATGATGAATTCAAAGATATGAAAGCGTCACTACGCGCCAGTTATCAGCATAATACCGAGAACGGTCGACTCTACCTCTCCGGTACATCACAGCTAGACAGTTATTATTCT                                                                                                                                     >  SRR19973070.1138427/1‑150 (MQ=60)
              GATCGAAATAATTCCTGGAGCGTTTCTGCTTCAGGCGACAATGATGAATTCAAAGATATGAAAGCGTCACTACGCGCCAGTTATCAGCATAATACCGAGAACGGTCGACTCTACCTCTCCGGTACATCACAGCTAGACAGTTATTATTCT                                                                                                                                     <  SRR19973070.795461/150‑1 (MQ=60)
               ATCGAAATAATTCCTGGAGCGTTTCTGCTTCAGGCGACAATGATGAATTCAAAGATATGAAAGCGTCACTACGCGCCAGTTATCAGCATAATACCGAGAACGGTCGACTCTACCTCTCCGGTACATCACAGCTAGACAGTTATTATTCTC                                                                                                                                    >  SRR19973070.737800/1‑150 (MQ=60)
               ATCGAAATAATTCCTGGAGCGTTTCTGCTTCAGGCGACAATGATGAATTCAAAGATATGAAAGCGTCACTACGCGCCAGTTATCAGCATAATACCGAGAACGGTCGACTCTACCTCTCCGGTACATCACAGCTAGACAGTTATTATTCTC                                                                                                                                    >  SRR19973070.1766161/1‑150 (MQ=60)
                TCGAAATAATTCCTGGAGCGTTTCTGCTTCAGGCGACAATGATGAATTCAAAGATATGAAAGCGTCACTACGCGCCAGTTATCAGCATAATACCGAGAACGGTCGACTCTACCTCTCCGGTACATCACAGCTAGACAGTTATTATTCTCT                                                                                                                                   <  SRR19973070.1277786/150‑1 (MQ=60)
                    AATAATTCCTGGAGCGTTTCTGCTTCAGGCGACAATGATGAATTCAAAGATATGAAAGCGTCACTACGCGCCAGTTATCAGCATAATACCGAGAACGGTCGACTCTACCTCTCCGGTACATCACAGCTAGACAGTTATTATTCTCTGAAT                                                                                                                               <  SRR19973070.86445/150‑1 (MQ=60)
                    AATAATTCCTGGAGCGTTTCTGCTTCAGGCGACAATGATGAATTCAAAGATATGAAAGCGTCACTACGCGCCAGTTATCAGCATAATACCGAGAACGGTCGACTCTACCTCTCCGGTACATCACAGCTAGACAGTTATTATTCTCTGAAT                                                                                                                               <  SRR19973070.688685/150‑1 (MQ=60)
                     ATAATTCCTGGAGCGTTTCTGCTTCAGGCGACAATTATGAATTCAAATATATGAAAGCGTCACTACGCGCCAGTTATCAGCATAATACCGAGAACGGTCGACTCTACCTCTCCGGTACATCACAGCTAGACAGTTATTATTCTCTGAATG                                                                                                                              <  SRR19973070.3315196/150‑1 (MQ=60)
                     ATAATTCCTGGAGCGTTTCTGCTTCAGGCGACAATGATGAATTCAAAGATATGAAAGCGTCACTACGCGCCAGTTATCAGCATAATACCGAGAACGGTCGACTCTACCTCTCCGGTACATCACAGCGAGACAGTTATTATTCTCTGAATG                                                                                                                              <  SRR19973070.1891133/150‑1 (MQ=60)
                     ATAATTCCTGGAGCGTTTCTGCTTCAGGCGACAATGATGAATTCAAAGATATGAAAGCGTCACTACGCGCCAGTTATCAGCATAATACCGAGAACGGTCGACTCTACCTCTCCGGTACATCACAGCGAGACAGTTATTATTCTCTGAATG                                                                                                                              <  SRR19973070.1891329/150‑1 (MQ=60)
                       AATTCCTGGAGCGTTTCTGCTTCAGGCGACAATGATGAATTCAAAGATATGAAAGCGTCACTACGCGCCAGTTATCAGCATAATACCGAGAACGGTCGACTCTACCTCTCCGGTACATCACAGCTAGACAGTTATTATTCTCTGAATGCC                                                                                                                            <  SRR19973070.196995/150‑1 (MQ=60)
                           CCTGGAGCGTTTCTGCTTCAGGCGACAATGATGAATTCAAAGATATGAAAGCGTCACTACGCGCCAGTTATCAGCATAATACCGAGAACGGTCGACTCTACCTCTCCGGTACATCACAGCTAGACAGTTATTATTCTCTGAATGCCAGTT                                                                                                                        <  SRR19973070.2210709/150‑1 (MQ=60)
                            CTGGAGCGTTTCTGCTTCAGGCGACAATGATGAATTCAAAGATATGAAAGCGTCACTACGCGCCAGTTATCAGCATAATACCGAGAACGGTCGACTCTACCTCTCCGGTACATCACAGCTAGACAGTTATTATTCTCTGAATGCCAGTTG                                                                                                                       <  SRR19973070.1342504/150‑1 (MQ=60)
                                    TTTCTGCTTCAGGCGACAATGATGAATTCAAAGATATGAAAGCGTCACTACGCGCCAGTTATCAGCATAATACCGAGAACGGTCGACTCTACCTCTCCGGTACATCACAGCTAGACAGTTATTATTCTCTGAATGCCAGTTGGAATGGTT                                                                                                               <  SRR19973070.1873846/150‑1 (MQ=60)
                                    TTTCTGCTTCAGGCGACAATGATGAATTCAAAGATATGAAAGCGTCACTACGCGCCAGTTATCAGCATAATACCGAGAACGGTCGACTCTACCTCTCCGGTACATCACAGCTAGACAGTTATTATTCTCTGAATGCCAGTTGGAATGGTT                                                                                                               <  SRR19973070.1633352/150‑1 (MQ=60)
                                      TCTGCTTCAGGCGACAATGATGAATTCAAAGATATGAAAGCGTCACTACGCGCCAGTTATCAGCATAATACCGAGAACGGTCGACTCTACCTCTCCGGTACATCACAGCTAGACAGTTATTATTCTCTGAATGCCAGTTGGAATGGTTCA                                                                                                             >  SRR19973070.2567278/1‑150 (MQ=60)
                                            TCAGGCGACAATGATGAATTCAAAGATATGAAAGCGTCACTACGCGCCAGTTATCAGCATAATACCGAGAACGGTCGACTCTACCTCTCCGGTACATCACAGCTAGACAGTTATTATTCTCTGAATGCCAGTTGGAATGGTTCATTCACT                                                                                                       <  SRR19973070.2686640/150‑1 (MQ=60)
                                                 CGACAATGATGAATTCAAAGATATGAAAGCGTCACTACTCGCCAGTTATCAGCATAATACCGAGAACGGTCGACTCTACATCTCCGGTACATCACAGCGATACACTTAATATTCTCTGAATTCCAGTAGGAATGGTTCATTCACTTCGAC                                                                                                  >  SRR19973070.1664071/1‑150 (MQ=60)
                                                 CGACAATGATGAATTCAAAGATATGAAAGCGTCACTACGCGCCAGTTATCAGCATAATACCGAGAACGGTCGACTCTACCTCTCCGGTACATCACAGCGAGACAGTTATTATTCTCTGAATGCCAGTTGGAATGGTTCATTCACTGCGAC                                                                                                  >  SRR19973070.2941687/1‑150 (MQ=60)
                                                  GACAATGATGAATTCAAAGATATGAAAGCGTCACTACGCGCCAGTTATCAGCATAATACCGAGAACGGTCGACTCTACCTCTCCGGTACATCACAGCTAGACAGTTATTATTCTCTGAATGCCAGTTGGAATGGTTCATTCACTGCGACT                                                                                                 >  SRR19973070.2603687/1‑150 (MQ=60)
                                                   ACAATGATGAATTCAAAGATATGAAAGCGTCACTACGCGCCAGTTATCAGCATAATACCGAGAACGGTCGACTCTACCTCTCCGGTACATCACAGCTAGACAGTTATTATTCTCTGAATGCCAGTTGGAATGGTTCATTCACTGCGACTC                                                                                                >  SRR19973070.1912714/1‑150 (MQ=60)
                                                        GATGAATTCAAAGATATGAAAGCGTCACTACGCGCCAGTTATCAGCATAATACCGAGAACGGTCGACTCTACCTCTCCGGTACATCACAGCTAGACAGTTATTATTCTCTGAATGCCAGTTGGAATGGTTCATTCACTGCGACTCGCCAC                                                                                           >  SRR19973070.2428306/1‑150 (MQ=60)
                                                         ATGAATTCAAAGATATGAAAGCGTCACTACGCGCCAGTTATCAGCATAATACCGAGAACGGTCGACTCTACCTCTCCGGTACATCACAGCTAGACAGTTATTATTCTCTGAATGCCAGTTGGAATGGTTCATTCACTGCGACTCGCCACG                                                                                          >  SRR19973070.2248258/1‑150 (MQ=60)
                                                          TGAATTCAAAGATATGAAAGCGTCACTACGCGCCAGTTATCAGCATAATACCGAGAACGGTCGACTCTACCTCTCCGGTACATCACAGCTAGACAGTTATTATTCTCTGAATGCCAGTTGGAATGGTTCATTCACTGCGACTCGCCACGG                                                                                         >  SRR19973070.961078/1‑150 (MQ=60)
                                                          TGAATTCAAAGATATGAAAGCGTCACTACGCGCCAGTTATCAGCATAATACCGAGAACGGTCGACTCTACCTCTCCGGTACATCACAGCTAGACAGTTATTATTCTCTGAATGCCAGTTGGAATGGTTCATTCACTGCGACTCGCCACGG                                                                                         >  SRR19973070.638251/1‑150 (MQ=60)
                                                             ATTCAAAGATATGAAAGCGTCACTACGCGCCAGTTATCAGCATAATACCGAGAACGGTCGACTCTACCTCTCCGGTACATCACAGCTAGACAGTTATTATTCTCTGAATGCCAGTTGGAATGGTTCATTCACTGCGACTCGCCACGGTGC                                                                                      <  SRR19973070.2459801/150‑1 (MQ=60)
                                                             ATTCAAAGATATGAAAGCGTCACTACGCGCCAGTTATCAGCATAATACCGAGAACGGTCGACTCTACCTCTCCGGTACATCACAGCTAGACAGTTATTATTCTCTGAATGCCAGTTGGAATGGTTCATTCACTGCGACTCGCCACGGTGC                                                                                      >  SRR19973070.1015640/1‑150 (MQ=60)
                                                             ATTCAAAGATATGAAAGCGTCACTACGCGCCAGTTATCAGCATAATACCGAGAACGGTCGACTCTACCTCTCCGGTACATCACAGCTAGACAGTTATTATTCTCTGAATGCCAGTTGGAATGGTTCATTCACTGCGACTCGCCACGGTGC                                                                                      >  SRR19973070.1015675/1‑150 (MQ=60)
                                                              TTCAAAGATATGAAAGCGTCACTACGCGCCAGTTATCAGCATAATACCGAGAACGGTCGACTCTACCTCTCCGGTACATCACAGCTAGACAGTTATTATTCTCTGAATGCCAGTTGGAATGGTTCATTCACTGCGACTCGCCACGGTGCC                                                                                     >  SRR19973070.3194386/1‑150 (MQ=60)
                                                              TTCAAAGATATGAAAGCGTCACTACGCGCCAGTTATCAGCATAATACCGAGAACGGTCGACTCTACCTCTCCGGTACATCACAGCTAGACAGTTATTATTCTCTGAATGCCAGTTGGAATGGTTCATTCACTGCGACTCGCCACGGTGCC                                                                                     >  SRR19973070.333304/1‑150 (MQ=60)
                                                              TTCAAAGATATGAAAGCGTCACTACGCGCCAGTTATCAGCATAATACCGAGAACGGTCGACTCTACCTCTCCGGTACATCACAGCTAGACAGTTATTATTCTCTGAATGCCAGTTGGAATGGTTCATTCACTGCGACTCGCCACGGTGCC                                                                                     >  SRR19973070.1543982/1‑150 (MQ=60)
                                                                     ATATGAAAGCGTCACTACGCGCCAGTTATCAGCATAATACCGAGAACGGTCGACTCTACCTCTCCGGTACATCACAGCTAGACAGTTATTATTCTCTGAATGCCAGTTGGAATGGTTCATTCACTGCGACTCGCCACGGTGCCGCTTTCC                                                                              >  SRR19973070.696965/1‑150 (MQ=60)
                                                                     ATATGAAAGCGTCACTACGCGCCAGTTATCAGCATAATACCGAGAACGGTCGACTCTACCTCTCCGGTACATCACAGCTAGACAGTTATTATTCTCTGAATGCCAGTTGGAATGGTTCATTCACTGCGACTCGCCACGGTGCCGCTTTCC                                                                              >  SRR19973070.1280418/1‑150 (MQ=60)
                                                                     ATATGAAAGCGCCACTACGCGCCAGTTATCAGCATAATACCGAGAACGGTCGACTCTACCTCTCCGGTACATCACAGCTAGACAGTTATTATTCTCTGAATGCCAGTTGGAATGGTTCATTCACTGCGACTCGCCACGGTGCCGCTTTCC                                                                              >  SRR19973070.2782482/1‑150 (MQ=60)
                                                                     ATATGAAAGCGCCACTACGCGCCAGTTATCAGCATAATACCGAGAACGGTCGACTCTACCTCTCCGGTACATCACAGCTAGACAGTTATTATTCTCTGAATGCCAGTTGGAATGGTTCATTCACTGCGACTCGCCACGGTGCCGCTTTCC                                                                              >  SRR19973070.2782412/1‑150 (MQ=60)
                                                                       ATGAAAGCGTCACTACGCGCCAGTTATCAGCATAATACCGAGAACGGTCGACTCTACCTCTCCGGTACATCACAGCTAGACAGTTATTATTCTCTGAATGCCAGTTGGAATGGTTCATTCACTGCGACTCGCCACGGTGCCGCTTTCCAC                                                                            >  SRR19973070.521492/1‑150 (MQ=60)
                                                                       ATGAAAGCGTCACTACGCGCCAGTTATCAGCATAATACCGAGAACGGTCGACTCTACCTCTCCGGTACATCACAGCTAGACAGTTATTATTCTCTGAATGCCAGTTGGAATGGTTCATTCACTGCGACTCGCCACGGTGCCGCTTTCCAC                                                                            >  SRR19973070.521457/1‑150 (MQ=60)
                                                                        TGAAAGCGTCACTACGCGCCAGTTATCAGCAAAATACCGAGAACGGTCGACTCTACCTCTCCGGTACATCACAGCTAGACAGTTATTATTCTCTGAATGCCAGTTGGAATGGTTCATTCACTGCGACTCGCCACGGTGCCGCTTTCCACG                                                                           >  SRR19973070.2832140/1‑150 (MQ=60)
                                                                           AAGCGTCACTACGCGCCAGTTATCAGCATAATACCGAGAACGGTCGACTCTACCTCTCCGGTACATCACAGCTAGACAGTTATTATTCTCTGAATGCCAGTTGGAATGGTTCATTCACTGCGACTCGCCACGGTGCCGCTTTCCACGACT                                                                        >  SRR19973070.1753556/1‑150 (MQ=60)
                                                                            AGCGTCACTACGCGCCAGTTATCAGCATAATACCGAGAACGGTCGACTCTACCTCTCCGGTACATCACAGCTAGACAGTTATTATTCTCTGAATGCCAGTTGGAATGGTTCATTCACTGCGACTCGCCACGGTGCCGCTTTCCACGACTA                                                                       >  SRR19973070.2429862/1‑150 (MQ=60)
                                                                              CGTCACTACGCGCCAGTTATCAGCATAATACCGAGAACGGTCGACTCTACCTCTCCGGTACATCACAGCTAGACAGTTATTATTCTCTGAATGCCAGTTGGAATGGTTCATTCACTGCGACTCGCCACGGTGCCGCTTTCCACGACTATA                                                                     >  SRR19973070.3043975/1‑150 (MQ=60)
                                                                              CGTCACTACGCGCCAGTTATCAGCATAATACCGAGAACGGTCGACTCTACCTCTCCGGTACATCACAGCTAGACAGTTATTATTCTCTGAATGCCAGTTGGAATGGTTCATTCACTGCGACTCGCCACGGTGCCGCTTTCCACGACTATA                                                                     >  SRR19973070.1564512/1‑150 (MQ=60)
                                                                                TCACTACGCGCCAGTTATCAGCATAATACCGAGAACGGTCGACTCTACCTCTCCGGTACATCACAGCTAGACAGTTATTATTCTCTGAATGCCAGTTGGAATGGTTCATTCACTGCGACTCGCCACGGTGCCGCTTTCCACGACTATAGC                                                                   >  SRR19973070.2899521/1‑150 (MQ=60)
                                                                                TCACTACGCGCCAGTTATCAGCATAATACCGAGAACGGTCGACTCTACATCTCCGGTACATCACAGCTAGACAGTTATTATTCTCTGAATGCCAGTTGGAATGGTTCATTCACTGCGACTCGCCACGGTGCCGCTTTCCACGACTATAGC                                                                   >  SRR19973070.2899064/1‑150 (MQ=60)
                                                                                   CTACGCGCCAGTTATCAGCATAATACCGCGAACGGTCGACTCTACCTCTCCGGTACATCACAGCTAGACAGTTATTATTCTCTGAATGCCAGTTGGAATGGTTCATTCACTGCGACTCGCCACGGTGCCGCTTTCCACGACTATAGCGGT                                                                <  SRR19973070.1672445/150‑1 (MQ=60)
                                                                                    TACGCGCCAGTTATCAGCATAATACCGAGAACGGTCGACTCTACCTCTCCGGTACATCACAGCTAGACAGTTATTATTCTCTGAATGCCAGTTGGAATGGTTCATTCACTGCGACTCGCCACGGTGCCGCTTTCCACGACTATAGCGGTA                                                               <  SRR19973070.757941/150‑1 (MQ=60)
                                                                                    TACGCGCCAGTTATCAGCATAATACCGAGAACGGTCGACTCTACCTCTCCGGTACATCACAGCTAGACAGTTATTATTCTCTGAATGCCAGTTGGAATGGTTCATTCACTGCGACTCGCCACGGTGCCGCTTTCCACGACTATAGCGGTA                                                               <  SRR19973070.758094/150‑1 (MQ=60)
                                                                                       GCGCCAGTTATCAGCATAATACCGAGAACGGTCGACTCTACCTCTCCGGTACATCACAGCTAGACAGTTATTATTCTCTGAATGCCAGTTGGAATGGTTCATTCACTGCGACTCGCCACGGTGCCGCTTTCCACGACTATAGCGGTAGTG                                                            <  SRR19973070.2654368/150‑1 (MQ=60)
                                                                                       GCGCCAGTTATCAGCATAATACCGAGAACGGTCGACTCTACCTCTCCGGTACATCACAGCTAGACAGTTATTATTCTCTGAATGCCAGTTGGAATGGTTCATTCACTGCGACTCGCCACGGTGCCGCTTTCCACGACTATAGCGGTAGTG                                                            <  SRR19973070.543874/150‑1 (MQ=60)
                                                                                        CGCCAGTTATCAGCATAATACCGAGAACGGTCGACTCTACCTCTCCGGTACATCACAGCTAGACAGTTATTATTCTCTGAATGCCAGTTGGAATGGTTCATTCACTGCGACTCGCCACGGTGCCGCTTTCCACGACTATAGCGGTAGTGC                                                           >  SRR19973070.2659560/1‑150 (MQ=60)
                                                                                        CGCCAGTTATCAGCATAATACCGAGAACGGTCGACTCTACCTCTCCGGTACATCACAGCTAGACAGTTATTATTCTCTGAATGCCAGTTGGAATGGTTCATTCACTGCGACTCGCCACGGTGCCGCTTTCCACGACTATAGCGGTAGTGC                                                           >  SRR19973070.2196130/1‑150 (MQ=60)
                                                                                            AGTTATCAGCATAATACCGAGAACGGTCGACTCTACCTCTCCGGTACATCACAGCTAGACAGTTATTATTCTCTGAATGCCAGTTGGAATGGTTCATTCACTGCGACTCGCCACGGTGCCGCTTTCCACGACTATAGCGGTAGTGCTGAC                                                       >  SRR19973070.2539139/1‑150 (MQ=60)
                                                                                            AGTTATCAGCATAATACCGAGAACGGTCGACTCTACCTCTCCGGTACATCACAGCTAGACAGTTATTATTCTCTGAATGCCAGTTGGAATGGTTCATTCACTGCGACTCGCCACGGTGCCGCTTTCCACGACTATAGCGGTAGTGCTGAC                                                       >  SRR19973070.1255751/1‑150 (MQ=60)
                                                                                            AGTTATCAGCATAATACCGAGAACGGTCGACTCTACCTCTCCGGTACATCACAGCTAGACAGTTATTATTCTCTGAATGCCAGTTGGAATGGTTCATTCACTGCGACTCGCCACGGTGCCGCTTTCCACGACTATAGCGGTAGTGCTGAC                                                       >  SRR19973070.871221/1‑150 (MQ=60)
                                                                                               TATCAGCATAATACCGAGAACGGTCGACTCTACCTCTCCGGTACATCACAGCTAGACAGTTATTATTCTCTGAATGCCAGTTGGAATGGTTCATTCACTGCGACTCGCCACGGTGCCGCTTTCCACGACTATAGCGGTAGTGCTGACTCG                                                    >  SRR19973070.2844930/1‑150 (MQ=60)
                                                                                                  CAGCATAATACCGAGAACGGTCGACTCTACCTCTCCGGTACATCACAGCTAGACAGTTATTATTCTCTGAATGCCAGTTGGAATGGTTCATTCACTGCGACTCGCCACGGTGCCGCTTTCCACGACTATAGCGGTAGTGCTGACTCGCGT                                                 >  SRR19973070.3159818/1‑150 (MQ=60)
                                                                                                   AGCATAATACCGAGAACGGTCGACTCTACCTCTCCGGTACATCACAGCGAGACAGTTATTATTCTCTGAATGCCAGTTGGAATGGTTCATTCACTGCGACTCGCCACGGTGCCGCTTTCCACGACTATAGCGGTAGTGCTGACTCGCGTG                                                >  SRR19973070.2707400/1‑150 (MQ=60)
                                                                                                      ATAATACCGAGAACGGTCGACTCTACCTCTCCGGTACATCACAGCTAGACAGTTATTATTCTCTGAATGCCAGTTGGAATGGTTCATTCACTGCGACTCGCCACGGTGCCGCTTTCCACGACTATAGCGGTAGTGCTGACTCGCGTTTTA                                             >  SRR19973070.1920149/1‑150 (MQ=60)
                                                                                                       TAATACCGAGAACGGTCGACTCTACCTCTCCGGTACATCACAGCTAGACAGTTATTATTCTCTGAATGCCAGTTGGAATGGTTCATTCACTGCGACTCGCCACGGTGCCGCTTTCCACGACTATAGCGGTAGTGCTGACTCGCGTTTTAT                                            <  SRR19973070.1154898/150‑1 (MQ=60)
                                                                                                        AATACCGAGAACGGTCGACTCTACCTCTCCGGTACATCACAGCTAGACAGTTATTATTCTCTGAATGCCAGTTGGAATGGTTCATTCACTGCGACTCGCCACGGTGCCGCTTTCCACGACTATAGCGGTAGTGCTGACTCGCGTTTTATG                                           >  SRR19973070.3098954/1‑150 (MQ=60)
                                                                                                        AATACCGAGAACGGTCGACTCTACCTCTCCGGTACATCACAGCTAGACAGTTATTATTCTCTGAATGCCAGTTGGAATGGTTCATTCACTGCGACTCGCCACGGTGCCGCTTTCCACGACTATAGCGGTAGTGCTGACTCGCGTTTTATG                                           <  SRR19973070.1115869/150‑1 (MQ=60)
                                                                                                        AATACCGAGAACGGTCGACTCTACCTCTCCGGTACATCACAGCTAGACAGTTATTATTCTCTGAATGCCAGTTGGAATGGTTCATTCACTGCGACTCGCCACGGTGCCGCTTTCCACGACTATAGCGGTAGTGCTGACTCGCGTTTTATG                                           <  SRR19973070.2515993/150‑1 (MQ=60)
                                                                                                              GNGAACGGTCGACTCTACCTCTCCGGTACATCACAGCTAGACAGTTATTATTCTCTGAATGCCAGTTGGAATGGTTCATTCACTGCGACTCGCCACGGTGCCGCTTTCCACGACTATAGCGGTAGTGCTGACTCGCGTTTTATGATCGAC                                     >  SRR19973070.1813270/1‑150 (MQ=60)
                                                                                                              GAGAACGGTCGACTCTACCTCTCCGGTACATCACAGCTAGACAGTTATTATTCTCTGAATGCCAGTTGGAATGGTTCATTCACTGCGACTCGCCACGGTGCCGCTTTCCACGACTATAGCGGTAGTGCTGACTCGCGTTTTATGATCGAC                                     >  SRR19973070.2116128/1‑150 (MQ=60)
                                                                                                              GAGAACGGTCGACTCTACCTCTCCGGTACATCACAGCTAGACAGTTATTATTCTCTGAATGCCAGTTGGAATGGTTCATTCACTGCGACTCGCCACGGTGCCGCTTTCCACGACTATAGCGGTAGTGCTGACTCGCGTTTTATGATCGAC                                     >  SRR19973070.2115921/1‑150 (MQ=60)
                                                                                                              GAGAACGGTCGACTCTACCTCTCCGGTACATCACAGCTAGACAGTTATTATTCTCTGAATGCCAGTTGGAATGGTTCATTCACTGCGACTCGCCACGGTGCCGCTTTCCACGACTATAGCGGTAGTGCTGACTCGCGTTTTATGATCGAC                                     >  SRR19973070.439650/1‑150 (MQ=60)
                                                                                                               AGAACGGTCGACTCTACCTCTCCGGTACATCACAGCGAGACAGTTATTATTCTCTGAATGCCAGTTGGAATGGTTCATTCACTGCGACTCGCCACGGTGCCGCTTTCCACGACTATAGCGGTAGTGCTGACTCGCGTTTTATGATCGACG                                    <  SRR19973070.456729/150‑1 (MQ=60)
                                                                                                                GAACGGTCGACTCTACCTCTCCGGTACATCACAGCTAGACAGTTATTATTCTCTGAATGCCAGTTGGAATGGTTCATTCACTGCGACTCGCCACGGTGCCGCTTTCCACGACTATAGCGGTAGTGCTGACTCGCGTTTTATGATCGACGC                                   >  SRR19973070.1058680/1‑150 (MQ=60)
                                                                                                                    GGTCGACTCTACCTCTCCGGTACATCACAGCTAGACAGTTATTATTCTCTGAATGCCAGTTGGAATGGTTCATTCACTGCGACTCGCCACGGTGCCGCTTTCCACGACTATAGCGGTAGTGCTGACTCGCGTTTTATGATCGACGCAGAC                               <  SRR19973070.2987648/150‑1 (MQ=60)
                                                                                                                    GGTCGACTCTACCTCTCCGGTACATCACAGCTAGACAGTTATTATTCTCTGAATGCCAGTTGGAATGGTTCATTCACTGCGACTCGCCACGGTGCCGCTTTCCACGACTATAGCGGTAGTGCTGACTCGCGTTTTATGATCGACGCAGAC                               <  SRR19973070.1915771/150‑1 (MQ=60)
                                                                                                                       CGACTCTACCTCTCCGGTACATCACAGCGAGACAGTTATTATTCTCTGAATGCCAGTTGGAATGGTTCATTCACTGCGACTCGCCACGGTGCCGCTTTCCACGACTATAGCGGTAGTGCTGACTCGCGTTTTATGATCGACGCAGACGGC                            >  SRR19973070.2957825/1‑150 (MQ=60)
                                                                                                                         ACTCTACCTCTCCGGTACATCACAGCTAGACAGTTATTATTCTCTGAATGCCAGTTGGAATGGTTCATTCACTGCGACTCGCCACGGTGCCGCTTTCCACGACTATAGCGGTAGTGCTGACTCGCGTTTTATGATCGACGCAGACGGCAC                          >  SRR19973070.3140238/1‑150 (MQ=60)
                                                                                                                          CTCTACCTCTCCGGTACATCACAGCTAGACAGTTATTATTCTCTGAATGCCAGTTGGAATGGTTCATTCACTGCGACTCGCCACGGTGCCGCTTTCCACGACTATAGCGGTAGTGCTGACTCGCGTTTTATGATCGACGCAGACGGCACT                         <  SRR19973070.1757455/150‑1 (MQ=60)
                                                                                                                             TACCTCTCCGGTACATCACAGCTAGACAGTTATTATTCTCTGAATGCCAGTTGGAATGGTTCATTCACTGCGACTCGCCACGGTGCCGCTTTCCACGACTATAGCGGTAGTGCTGACTCGCGTTTTATGATCGACGCAGACGGCACTGAA                      >  SRR19973070.964772/1‑150 (MQ=60)
                                                                                                                             TACCTCTCCGGTACATCACAGCTAGACAGTTATTATTCTCTGAATGCCAGTTGGAATGGTTCATTCACTGCGACTCGCCACGGTGCCGCTTTCCACGACTATAGCGGTAGTGCTGACTCGCGTTTTATGATCGACGCAGACGGCACTGAA                      >  SRR19973070.1084356/1‑150 (MQ=60)
                                                                                                                              ACCTCTCCGGTACATCACCGCGAGACAGTTATTATTCTCTGAATGCCAGTTGGAATGGTTCATTCACTGCGACTCGCCACGGTGCCGCTTTCCACGACTATAGCGGTAGTGCTGACTCGCGTTTTATGATCGACGCAGACGGCACTGAAG                     <  SRR19973070.661319/150‑1 (MQ=60)
                                                                                                                              ACCTCTCCGGTACATCACAGCGAGACAGTTATTATTCTCTGAATGCCAGTTGGAATGGTTCATTCACTGCGACTCGCCACGGTGCCGCTTTCCACGACTATAGCGGTAGTGCTGACTCGCGTTTTATGATCGACGCAGACGGCACTGAAG                     <  SRR19973070.661151/150‑1 (MQ=60)
                                                                                                                                CTCTCCGGTACATCACAGCTAGACAGTTATTATTCTCTGAATGCCAGTTGGAATGGTTCATTCACTGCGACTCGCCACGGTGCCGCTTTCCACGACTATAGCGGTAGTGCTGACTCGCGTTTTATGATCGACGCAGACGGCACTGAAGAT                   >  SRR19973070.1574137/1‑150 (MQ=60)
                                                                                                                                  CTCCGGTACATCACAGCTAGACAGTTATTATTCTCTGAATGCCAGTTGGAATGGTTCATTCACTGCGACTCGCCACGGTGCCGCTTTCCACGACTATAGCGGTAGTGCTGACTCGCGTTTTATGATCGACGCAGACGGCACTGAAGATAT                 <  SRR19973070.1586445/150‑1 (MQ=60)
                                                                                                                                  CTCCGGTACATCACAGCTAGACAGTTATTATTCTCTGAATGCCAGTTGGAATGGTTCATTCACTGCGACTCGCCACGGTGCCGCTTTCCACGACTATAGCGGTAGTGCTGACTCGCGTTTTATGATCGACGCAGACGGCACTGAAGATAT                 >  SRR19973070.755662/1‑150 (MQ=60)
                                                                                                                                   TCCGGTACATCACAGCTAGACAGTTATTATTCTCTGAATGCCAGTTGGAATGGTTCATTCACTGCGACTCGCCACGGTGCCGCTTTCCACGACTATAGCGGTAGTGCTGACTCGCGTTTTATGATCGACGCAGACGGCACTGAAGATATT                <  SRR19973070.495961/150‑1 (MQ=60)
                                                                                                                                   TCCGGTACATCACAGCTAGACAGTTATTATTCTCTGAATGCCAGTTGGAATGGTTCATTCACTGCGACTCGCCACGGTGCCGCTTTCCACGACTATAGCGGTAGTGCTGACTCGCGTTTTATGATCGACGCAGACGGCACTGAAGATATT                <  SRR19973070.495962/150‑1 (MQ=60)
                                                                                                                                   TCCGGTACATCACAGCTAGACAGTTATTATTCTCTGAATGCCAGTTGGAATGGTTCATTCACTGCGACTCGCCACGGTGCCGCTTTCCACGACTATAGCGGTAGTGCTGACTCGCGTTTTATGATCGACGCAGACGGCACTGAAGATATT                <  SRR19973070.1871560/150‑1 (MQ=60)
                                                                                                                                    CCGGTACATCACAGCTAGACAGTTATTATTCTCTGAATGCCAGTTGGAATGGTTCATTCACTGCGACTCGCCACGGTGCCGCTTTCCACGACTATAGCGGTAGTGCTGACTCGCGTTTTATGATCGACGCAGACGGCACTGAAGATATTC               <  SRR19973070.1779126/150‑1 (MQ=60)
                                                                                                                                     CGGTACATCACAGCGAGACAGTTATTATTCTCTGAATGCCAGTTGGAATGGTTCATTCACTGCGACTCGCCACGGTGCCGCTTTCCACGACTATAGCGGTAGTGCTGACTCGCGTTTTATGATCGACGCAGACGGCACTGAAGATATTCC              <  SRR19973070.2332361/150‑1 (MQ=60)
                                                                                                                                      GGTACATCACAGCTAGACAGTTATTATTCTCTGAATGCCAGTTGGAATGGTTCATTCACTGCGACTCGCCACGGTGCCGCTTTCCACGACTATAGCGGTAGTGCTGACTCGCGTTTTATGATCGACGCAGACGGCACTGAAGATATTCCG             >  SRR19973070.1007907/1‑150 (MQ=60)
                                                                                                                                      GGTACATCACAGCTAGACAGTTATTATTCTCTGAATGCCAGTTGGAATGGTTCATTCACTGCGACTCGCCACGGTGCCGCTTTCCACGACTATAGCGGTAGTGCTGACTCGCGTTTTATGATCGACGCAGACGGCACTGAAGATATTCCG             >  SRR19973070.2736551/1‑150 (MQ=60)
                                                                                                                                        TACATCACAGCTAGACAGTTATTATTCTCTGAATGCCAGTTGGAATGGTTCATTCACTGCGACTCGCCACGGTGCCGCTTTCCACGACTATAGCGGTAGTGCTGACTCGCGTTTTATGATCGACGCAGACGGCACTGAAGATATTCCGTT           >  SRR19973070.1309579/1‑150 (MQ=60)
                                                                                                                                          CATCACAGCTAGACAGTTATTATTCTCTGAATGCCAGTTGGAATGGTTCATTCACTGCGACTCGCCACGGTGCCGCTTTCCACGACTATAGCGGTAGTGCTGACTCGCGTTTTATGATCGACGCAGACGGCACTGAAGATATTCCGTTGA         >  SRR19973070.2230626/1‑150 (MQ=60)
                                                                                                                                          CATCACAGCTAGACAGTTATTATTCTCTGAATGCCAGTTGGAATGGTTCATTCACTGCGACTCGCCACGGTGCCGCTTTCCACGACTATAGCGGTAGTGCTGACTCGCGTTTTATGATCGACGCAGACGGCACTGAAGATATTCCGTTGA         <  SRR19973070.409056/150‑1 (MQ=60)
                                                                                                                                             CACAGCTAGACAGTTATTATTCTCTGAATGCCAGTTGGAATGGTTCATTCACTGCGACTCGCCACGGTGCCGCTTTCCACGACTATAGCGGTAGTGCTGACTCGCGTTTTATGATCGACGCAGACGGCACTGAAGATATTCCGTTGAACA      <  SRR19973070.1107968/150‑1 (MQ=60)
                                                                                                                                               CAGCTAGACAGTTATTATTCTCTGAATGCCAGTTGGAATGGTTCATTCACTGCGACTCGCCACGGTGCCGCTTTCCACGACTATAGCGGTAGTGCTGACTCGCGTTTTATGATCGACGCAGACGGCACTGAAGATATTCCGTTGAACAAT    >  SRR19973070.286061/1‑150 (MQ=60)
                                                                                                                                               CAGCTAGACAGTTATTATTCTCTGAATGCCAGTTGGAATGGTTCATTCACTGCGACTCGCCACGGTGCCGCTTTCCACGACTATAGCGGTAGTGCTGACTCGCGTTTTATGATCGACGCAGACGGCACTGAAGATATTCCGTTGAACAAT    >  SRR19973070.1566661/1‑150 (MQ=60)
                                                                                                                                               CAGCTAGACAGTTATTATTCTCTGAATGCCAGTTGAAATGGTTCATTCACTGCGACTCGCCACGGTGCCGCTTTCCACGACTATAGCGGTAGTGCTGACTCGCGTTTTATGATCGACGCAGACGGCACTGAAGATATTCCGTTGAACAAT    <  SRR19973070.3079280/150‑1 (MQ=60)
                                                                                                                                                 GCTAGACAGTTATTATTCTCTGAATGCCAGTTGGAATGGTTCATTCACTGCGACTCGCCACGGTGCCGCTTTCCACGACTATAGCGGTAGTGCTGACTCGCGTTTTATGATCGACGCAGACGGCACTGAAGATATTCCGTTGAACAATAA  <  SRR19973070.2637370/150‑1 (MQ=60)
                                                                                                                                                 GCTAGACAGTTATTATTCTCTGAATGCCAGTTGGAATGGTTCATTCACTGCGACTCGCCACGGTGCCGCTTTCCACGACTATAGCGGTAGTGCTGACTCGCGTTTTATGATCGACGCAGACGGCACTGAAGATATTCCGTTGAACAATAA  <  SRR19973070.2637549/150‑1 (MQ=60)
                                                                                                                                                 GCGAGACAGTTATTATTCTCTGAATGCCAGTTGGAATGGTTCATTCACTGCGACTCGCCACGGTGCCGCTTTCCACGACTATAGCGGTAGTGCTGACTCGCGTTTTATGATCGACGCAGACGGCACTGAAGATATTCCGTTGAACAATAA  <  SRR19973070.2216537/150‑1 (MQ=60)
                                                                                                                                                   |                                                                                                                                                   
GGTATGACTCTTCCGATCGAAATAATTCCTGGAGCGTTTCTGCTTCAGGCGACAATGATGAATTCAAAGATATGAAAGCGTCACTACGCGCCAGTTATCAGCATAATACCGAGAACGGTCGACTCTACCTCTCCGGTACATCACAGCGAGACAGTTATTATTCTCTGAATGCCAGTTGGAATGGTTCATTCACTGCGACTCGCCACGGTGCCGCTTTCCACGACTATAGCGGTAGTGCTGACTCGCGTTTTATGATCGACGCAGACGGCACTGAAGATATTCCGTTGAACAATAA  >  CP009273/3182500‑3182794

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑