Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F368 I0 R1 1472 214.4 7116750 95.9% 6824963 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 618,241 G→T 51.1% S162S (TCG→TCT)  entS → enterobactin exporter, iron‑regulated

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273618,2410GT51.1% 63.7 / 281.9 160S162S (TCG→TCT) entSenterobactin exporter, iron‑regulated
Reads supporting (aligned to +/- strand):  ref base G (43/35);  new base T (40/42);  total (83/77)
Fisher's exact test for biased strand distribution p-value = 4.34e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.94e-01

Only 100 of 160 total aligned reads displayed.

ATTTACTTGGTTTATGGGATGGTTTTTTCGCATCGCTTGGCGTTACGGCGCTATTGGCGGCGACACCAGCACTGGTAGGGCGTGAAAACTTAATGCAGGCCGGGGCGATCACCATGTTGACCGTGCGTCTGGGGTCGGTGATTTCGCCCATGATTGGCGGTTTATTGCTGGCGACCGGTGGCGTAGCCTGGAACTACGGGCTGGCGGCGGCGGGCACGTTTATTACCTTGCTACCGTTGTTAAGCCTTCCGGCGTTGCCACCGCCACCGCAGCCGCGTGAGCATCCGTTGAA  >  CP009273/618096‑618387
                                                                                                                                                 |                                                                                                                                                  
  ttACTTGGTTTATGGGATGGTTTTTTCGCATCGCTTGGCGTTACGGCGCTATTGGCGGCGACACCAGCACTGGTAGGGCGTGAAAACTTAATGCAGGCCGGGGCGATCACCATGTTGACCGTGCGTCTGGGGTCGGTGATTTCTCCCa                                                                                                                                                >  1:2602133/1‑148 (MQ=255)
  ttACTTGGTTTATGGGATGGTTTTTTCGCATCGCTTGGCGTTACGGCGCTATTGGCGGCGACACCAGCACTGGTAGGGCGTGAAAACTTAATGCAGGCCGGGGCGATCACCATGTTGACCGTGCGTCTGGGGTCGGTGATTTCTCCCa                                                                                                                                                >  1:2602106/1‑148 (MQ=255)
       tGGTTTATGGGATGGTTTTTTCGCATCGCTTGGCGTTACGGCGCTATTGGCGGCGACACCAGCACTGGTAGGGCGTGAAAACTTAATGCAGGCCGGGGCGATCACCATGTTGACCGTGCGTCTGGGGTCGGTGATTTCTCCCATGAtt                                                                                                                                           >  2:2724699/1‑148 (MQ=255)
       tGGTTTATGGGATGGTTTTTTCGCATCGCTTGGCGTTACGGCGCTATTGGCGGCGACACCAGCACTGGTAGGGCGTGAAAACTTAATGCAGGCCGGGGCGATCACCATGTTGACCGTGCGTCTGGGGTCGGTGATTTCGCCCATGAtt                                                                                                                                           >  1:2288287/1‑148 (MQ=255)
       tGGTTTATGGGATGGTTTTTTCGCATCGCTAGGCGTTACGGCGCTATTGGCGGCGACACAAGCACTGGTAGGGCGTGAAAACTTAATGCAGGCCGGGTCGATCACCATGTTGACCGTGCTTCTGGGGTCGGTGATTTCTCCCATGAtt                                                                                                                                           >  1:3313452/1‑148 (MQ=255)
          tttATGGGATGGTTTTTTCGCATCGCTTGGCGTTACGGCGCTATTGGCGGCGACACCAGCACTGGTAGGGCGTGAAAACTTAATGCAGGCCGGGGCGATCACCATGTTGACCGTGCGTCTGGGGTCGGTGATTTCTCCCATGATTGGc                                                                                                                                        >  1:427886/1‑148 (MQ=255)
           ttATGGGATGGTTTTTTCGCATCGCTTGGCGTTACGGCGCTATTGGCGGCGACACCAGCACTGGTAGGGCGTGAAAACTTAATGCAGGCCGGGGCGATCACCATGTTGACCGTGCGTCTGGGGTCGGTGATTTCTCCCATGATTGGCg                                                                                                                                       <  2:700774/148‑1 (MQ=255)
           ttATGGGATGGTTTTTTCGCATCGCTTGGCGTTACGGCGCTATTGGCGGCGACACCAGCACTGGTAGGGCGTGAAAACTTAATGCAGGCCGGGGCGATCACCATGTTGACCGTGCGTCTGGGGTCGGTGATTTCTCCCATGATTGGCg                                                                                                                                       <  2:700735/148‑1 (MQ=255)
           ttATGGGATGGTTTTTTCGCATCGCTTGGCGTTACGGCGCTATTGGCGGCGACACCAGCACTGGTAGGGCGTGAAAACTTAATGCAGGCCGGGGCGATCACCATGTTGACCGTGCGTCTGGGGTCGGTGATTTCTCCCATGATTGGCg                                                                                                                                       <  2:2887227/148‑1 (MQ=255)
           ttATGGGATGGTTTTTTCGCATCGCTTGGCGTTACGGCGCTATTGGCGGCGACACCAGCACTGGTAGGGCGTGAAAACTTAATGCAGGCCGGGGCGATCACCATGTTGACCGTGCGTCTGGGGTCGGTGATTTCGCCCATGATTGGCg                                                                                                                                       >  1:1557882/1‑148 (MQ=255)
           ttATGGGATGGTTTTTTCGCATCGCTTGGCGTTACGGCGCTATTGGCGGCGACACCAGCACTGGTAGGGCGTGAAAACTTAATGCAGGCCGGGGCGATCACCATGTTGACCGTGCGTCTGGGGTCGGTGATTTCGCCCATGATTGGCg                                                                                                                                       >  1:1558689/1‑148 (MQ=255)
             aTGGGATGGTTTTTTCGCATCGCTTGGCGTTACGGCGCTATTGGCGGCGACACCAGCACTGGTAGGGCGTGAAAACTTAATGCAGGCCGGGGCGATCACCATGTTGACCGTGCGTCTGGGGTCGGTGATTTCGCCCATGATTGGCGGt                                                                                                                                     <  2:260698/148‑1 (MQ=255)
               gggATGGTTTTTTCGCATCGCTTGGCGTTACGGCGCTATTGGCGGCGACACCAGCACTGGTAGGGCGTGAAAACTTAATGCAGGCCGGGGCGATTACCATGTTGACCGTGCGTCTGGGGTCGGTGATTTCGCCCATGATTGGCGGttt                                                                                                                                   <  2:1492231/148‑1 (MQ=255)
               gggATGGTTTTTTCGCATCGCTTGGCGTTACGGCGCTATTGGCGGCGACACCAGCACTGGTAGGGCGTGAAAACTTAATGCAGGCCGGGGCGATCACCATGTTGACCGTGCGTCTGGGGTCGGTGATTTCGCCCATGATTGGCGGtgt                                                                                                                                   >  1:991202/1‑146 (MQ=255)
                 gATGGTTTTTTCGCATCGCTTGGCGTTACGGCGCTATTGGCGGCGACACCAGCACTGGTAGGGCGTGAAAACTTAATGCAGGCCGGGGCGATCACCATGTTGACCGTGCGTCTGGGGTCGGTGATTTCTCCCATGATTGGCGGTTTAt                                                                                                                                 >  1:2193433/1‑148 (MQ=255)
                 gATGGTTTTTTCGCATCGCTTGGCGTTACGGCGCTATTGGCGGCGACACCAGCACTGGTAGGGCGTGAAAACTTAATGCAGGCCGGGGCGATCACCATGTTGACCGTGCGTCTGGGGTCGGTGATTTCGCCCATGATTGGCGGTTTAt                                                                                                                                 <  1:2933037/148‑1 (MQ=255)
                   tGGTTTTTTCGCATCGCTTGGCGTTACGGCGCTATTGGCGGCGACACCAGCACTGGTAGGGCGTGAAAACTTAATGCAGGCCGGGGCGATCACCATGTTGACCGTGCGTCTGGGGTCGGTGATTTCGCCCATGATTGGCGGTTTATTg                                                                                                                               >  1:1214637/1‑148 (MQ=255)
                    ggTTTTTTCGCATCGCTTGGCGTTACGGCGCTATTGGCGGCGACACCAGCACTGGTAGGGCGTGAAAACTTAATGCAGGCCGGGGCGATCACCATGTTGACCGTGCGTCTGGGGTCGGTGATTTCGCCCATGATTGGCGGTTTATTGc                                                                                                                              <  1:184375/148‑1 (MQ=255)
                    ggTTTTTTCGCATCGCTTGGCGTTACGGCGCTATTGGCGGCGACACCAGCACTGGTAGGGCGTGAAAACTTAATGCAGGCCGGGGCGATCACCATGTTGACCGTGCGTCTGGGGTCGGTGATTTCGCCCATGATTGGCGGTTTATTGc                                                                                                                              >  2:2164942/1‑148 (MQ=255)
                           tCGCATCGCTTGGCGTTACGGCGCTATTGGCGGCGACACCAGCACTGGTAGGGCGTGAAAACTTAATGCAGGCCGGGGCGATCACCATGTTGACCGTGCGTCTGGGGTCGGTGATTTCGCCCATGATTGGCGGTTTATTGCTGGCGAc                                                                                                                       <  1:3319063/148‑1 (MQ=255)
                              cATCGCTTGGCGTTACGGCGCTATTGGCGGCGACACCAGCACTGGTAGGGCGTGAAAACTTAATGCAGGCCGGGGCGATCACCATGTTGACCGTGCGTCTGGGGTCGGTGATTTCTCCCATGATTGGCGGTTTATTGCTGGCGACCgg                                                                                                                    <  2:2199561/148‑1 (MQ=255)
                              cATCGCTTGGCGTTACGGCGCTATTGGCGGCGACACCAGCACTGGTAGGGCGTGAAAACTTAATGCAGGCCGGGGCGATCACCATGTTGACCGTGCGTCTGGGGTCGGTGATTTCGCCCATGATTGGCGGTTTATTGCTGGCGACCgg                                                                                                                    >  1:2488893/1‑148 (MQ=255)
                              cATCGCTTGGCGTTACGGCGCTATTGGCGGCGACACCAGCACTGGTAGGGCGTGAAAACTTAATGCAGGCCGGGGCGATCACCATGTTGACCGTGCGTCTGGGGTCGGTGATTTCGCCCATGATTGGCGGTTTATTGCTGGCGACCgg                                                                                                                    >  1:159916/1‑148 (MQ=255)
                                tCGCTTGGCGTTACGGCGCTATTGGCGGCGACACCAGCACTGGTAGGGCGTGAAAACTTAATGCAGGCCGGGGCGATCACCATGTTGACCGTGCGTCTGGGGTCGGTGATTTCGCCCATGATTGGCGGTTTATTGCTGGCGACCGGTg                                                                                                                  >  2:175587/1‑148 (MQ=255)
                                tCGCTTGGCGTTACGGCGCTATTGGCGGCGACACCAGCACTGGTAGGGCGTGAAAACTTAATGCAGGCCGGGGCGATCACCATGTTGACCGTGCGTCTGGGGTCGGTGATTTCGCCCATGATTGGCGGTTTATTGCTGGCGACCGGTg                                                                                                                  >  2:1156163/1‑148 (MQ=255)
                                 cGCTTGGCGTTACGGCGCTATTGGCGGCGACACCAGCACTGGTAGGGCGTGAAAACTTAATGCAGGCCGGGGCGATCACCATGTTGACCGTGCGTCTGGGGTCGGTGATTTCTCCCATGATTGGCGGTTTATTGCTGGCGACCGGTgg                                                                                                                 >  2:2654883/1‑148 (MQ=255)
                                     tGGCGTTACGGCGCTATTGGCGGCGACACCAGCACTGGTAGGGCGTGAAAACTTAATGCAGGCCGGGGCGATCACCATGTTGACCGTGCGTCTGGGGTCGGTGATTTCTCCCATGATTGGCGGTTTATTGCTGGCGACCGGTGGCGTa                                                                                                             >  2:3017569/1‑148 (MQ=255)
                                     tGGCGTTACGGCGCTATTGGCGGCGACACCAGCACTGGTAGGGCGTGAAAACTTAATGCAGGCCGGGGCGATCACCATGTTGACCGTGCGTCTGGGGTCGGTGATTTCTCCCATGATTGGCGGTTTATTGCTGGCGACCGGTGGCGTa                                                                                                             >  2:154559/1‑148 (MQ=255)
                                        cGTTACGGCGCTATTGGCGGCGACACCAGCACTGGTAGGGCGTGAAAACTTAATGCAGGCCGGGGCGATCACCATGTTGACCGTGCGTCTGGGGTCGGTGATTTCGCCCATGATTGGCGGTTTATTGCTGGCGACCGGTGGCGTAGcc                                                                                                          <  2:2940900/148‑1 (MQ=255)
                                           tACGGCGCTATTGGCGGCGACACCAGCACTGGTAGGGCGTGAAAACTTAATGCAGGCCGGGGCGATCACCATGTTGACCGTGCGTCTGGGGTCGGTGATTTCGCCCATGATTGGCGGTTTATTGCTGGCGACCGGTGGCGTAGCCTgg                                                                                                       >  2:634621/1‑148 (MQ=255)
                                           tACGGCGCTATTGGCGGCGACACCAGCACTGGTAGGGCGTGAAAACTTAATGCAGGCCGGGGCGATCACCATGTTGACCGTGCGTCTGGGGTCGGTGATTTCGCCCATGAGTGGCGGTTTATTGCTGGCGACCGGTGGCGTAGCCTgg                                                                                                       >  2:3246096/1‑148 (MQ=255)
                                            aCGGCGCTATTGGCGGCGACACCAGCACTGGTAGGGCGTGAAAACTTAATGCAGGCCGGGGCGATCACCATGTTGACCGTGCGTCTGGGGTCGGTGATTTCGCCCATGATTGGCGGTTTATTGCTGGCGACCGGTGGCGTAGCCTGGa                                                                                                      <  1:2489195/148‑1 (MQ=255)
                                            aCGGCGCTATTGGCGGCGACACCAGCACTGGTAGGGCGTGAAAACTTAATGCAGGCCGGGGCGATCACCATGTTGACCGTGCGTCTGGGGTCGGTGATTTCGCCCATGATTGGCGGTTTATTGCTGGCGACCGGTGGCGTAGCCTGGa                                                                                                      <  1:2491777/148‑1 (MQ=255)
                                                  cTATTGGCGGCGACACCAGCACTGGTAGGGCGTGAAAACTTAATGCAGGCCGGGGCGATCACCATGTTGACCGTGCGTCTGGGGTCGGTGATTTCGCCCATGATTGGCGGTTTATTGCTGGCGACCGGTGGCGTAGCCTGGAACTACg                                                                                                <  2:2260180/148‑1 (MQ=255)
                                                  cTATTGGCGGCGACACCAGCACTGGTAGGGCGTGAAAACTTAATGCAGGCCGGGGCGATCACCATGTTGACCGTGCGTCTGGGGTCGGTGATTTCGCCCATGATTGGCGGTTTATTGCTGGCGACCGGTGGCGTAGCCTGGAACTACg                                                                                                >  1:163697/1‑148 (MQ=255)
                                                     ttGGCGGCGACACCAGCACTGGTAGGGCGTGAAAACTTAATGCAGGCCGGGGCGATCACCATGTTGACCGTGCGTCTGGGGTCGGTGATTTCTCCCATGATTGGCGGTTTATTGCTGGCGACCGGTGGCGTAGCCTGGAACTACGGGc                                                                                             <  2:2193433/148‑1 (MQ=255)
                                                      tGGCGGCGACACCAGCACTGGTAGGGCGTGAAAACTTAATGCAGGCCGGGGCGATCACCATGTTGACCGTGCGTCTGGGGTCGGTGATTTCGCCCATGATTGGCGGTTTATTGCTGGCGACCGGTGGCGTAGCCTGGAACTACGGGCt                                                                                            <  2:1318123/148‑1 (MQ=255)
                                                           gcgACACCAGCACTGGTAGGGCGTGAAAACTTAATGCAGGCCGGGGCGATCACCATGTTGACCGTGCGTCTGGGGTCGGTGATTTCTCCCATGATTGGCGGTTTATTGCTGGCGACCGGTGGCGTAGCCTGGAACTACGGGCTggcgg                                                                                       <  2:3021059/148‑1 (MQ=255)
                                                           gcgACACCAGCACTGGTAGGGCGTGAAAACTTAATGCAGGCCGGGGCGATCACCATGTTGACCGTGCGTCTGGGGTCGGTGATTTCTCCCATGATTGGCGGTTTATTGCTGGCGACCGGTGGCGTAGCCTGGAACTACGGGCTggcgg                                                                                       <  1:2432973/148‑1 (MQ=255)
                                                            cgACACCAGCACTGGTAGGGCGTGAAAACTTAATGCAGGCCGGGGCGATCACCATGTTGACCGTGCGTCTGGGGTCGGTGATTTCTCCCATGATTGGCGGTTTATTGCTGGCGACCGGTGGCGTAGCCTGGAACTACGGGCTggcggc                                                                                      >  1:1573125/1‑148 (MQ=255)
                                                            cgACACCAGCACTGGTAGGGCGTGAAAACTTAATGCAGGCCGGGGCGATCACCATGTTGACCGTGCGTCTGGGGTCGGTGATTTCTCCCATGATTGGCGGTTTATTGCTGGCGACCGGTGGCGTAGCCTGGAACTACGGGCTggcggc                                                                                      >  1:1853140/1‑148 (MQ=255)
                                                            cgACACCAGCACTGGTAGGGCGTGAAAACTTAATGCAGGCCGGGGCGATCACCATGTTGACCGTGCGTCTGGGGTCGGTGATTTCGCCCATGATTGGCGGTTTATTGCTGGCGACCGGTGGCGTAGCCTGGAACTACGGGCTggcggc                                                                                      >  1:1324647/1‑148 (MQ=255)
                                                               cacCAGCACTGGTAGGGCGTGAAAACTTAATGCAGGCCGGGGCGATCACCATGTTGACCGTGCGTCTGGGGTCGGTGATTTCGCCCATGATTGGCGGATTATTGCTGGCGACCGGTGGCGTAGCCTGGAACTACGGCCTggcgggggg                                                                                   >  1:3399720/1‑147 (MQ=255)
                                                                acCAGCACTGGTAGGGCGTGAAAACTTAATGCAGGCCGGGGCGATCACCATGTTGACCGTGCGTCTGGGGTCGGTGATTTCTCCCATGATTGGCGGTTTATTGCTGGCGACCGGTGGCGTAGCCTGGAACTACGGGCTGgcggcggcg                                                                                  >  1:1955435/1‑148 (MQ=255)
                                                                  cAGCACTGGTAGGGCGTGAAAACTTAATGCAGGCCGGGGCGATCACCATGTTGACCGTGCGTCTGGGGTCGGTGATTTCGCCCATGATTGGCGGTTTATTGCTGGCGACCGGTGGCGTAGCCTGGAACTACGGGCTGGCGGCGGCggg                                                                                <  1:2003405/148‑1 (MQ=255)
                                                                    gCACTGGTAGGGCGTGAAAACTTAATGCAGGCCGGGGCGATCACCATGTTGACCGTGCGTCTGGGGTCGGTGATTTCTCCCATGATTGGCGGTTTATTGCTGGCGACCGGTGGCGTAGCCTGGAACTACGGGCTGGCGGCGGCGGGCa                                                                              >  2:207587/1‑148 (MQ=255)
                                                                     cACTGGTAGGGCGTGAAAACTTAATGCAGGCCGGGGCGATCACCATGTTGACCGTGCGTCTGGGGTCGGTGATTTCTCCCATGATTGGCGGTTTATTGCTGGCGACCGGTGGCGTAGCCTGGAACTACGGGCTGGCGGCGGCGGGCAc                                                                             >  1:291184/1‑148 (MQ=255)
                                                                      aCTGGTAGGGCGTGAAAACTTAATGCAGGCCGGGGCGATCACCATGTTGACCGTGCGTCTGGGGTCGGTGATTTCTCCCATGATTGGCGGTTTATTGCTGGCGACCGGTGGCGTAGCCTGGAACTACGGGCTGGCGGCGGCGGGCACg                                                                            <  1:2301515/148‑1 (MQ=255)
                                                                      aCTGGTAGGGCGTGAAAACTTAATGCAGGCCGGGGCGATCACCATGTTGACCGTGCGTCTGGGGTCGGTGATTTCTCCCATGATTGGCGGTTTATTGCTGGCGACCGGTGGCGTAGCCTGGAACTACGGGCTGGCGGCGGCGGGCACg                                                                            >  1:368081/1‑148 (MQ=255)
                                                                         ggTAGGGCGTGAAAACTTAATGCAGGCCGGGGCGATCACCATGTTGACCGTGCGTCTGGGGTCGGTGATTTCGCCCATGATTGGCGGTTTATTGCTGGCGACCGGTGGCGTAGCCTGGAACTACGGGCTGGCGGCGGCGGGCACGttt                                                                         <  1:3100542/148‑1 (MQ=255)
                                                                           tAGGGCGTGAAAACTTAATGCAGGCCGGGGCGATCACCATGTTGACCGTGCGTCTGGGGTCGGTGATTTCTCCCATGATTGGCGGTTTATTGCTGGCGACCGGTGGCGTAGCCTGGAACTACGGGCTGGCGGCGGCGGGCACGTttat                                                                       <  2:232092/148‑1 (MQ=255)
                                                                           tAGGGCGTGAAAACTTAATGCAGGCCGGGGCGATCACCATGTTGACCGTGCGTCTGGGGTCGGTGATTTCTCCCATGATTGGCGGTTTATTGCTGGCGACCGGTGGCGTAGCCTGGAACTACGGGCTGGCGGCGGCGGGCACGTttat                                                                       <  2:3492846/148‑1 (MQ=255)
                                                                           tAGGGCGTGAAAACTTAATGCAGGCCGGGGCGATCACCATGTTGACCGTGCGTCTGGGGTCGGTGATTTCGCCCATGATTGGCGGTTTATTGCTGGCGACCGGTGGCGTAGCCTGGAACTACGGGCTGGCGGCGGCGGGCACGTttat                                                                       >  2:3235951/1‑148 (MQ=255)
                                                                           tAGGGCGTGAAAACTTAATGCAGGCCGGGGCGATCACCATGTTGACCGTGCGTCTGGGGTCGGTGATTTCGCCCATGATTGGCGGTTTATTGCTGGCGACCGGTGGCGTAGCCTGGAACTACGGGCTGGCGGCGGCGGGCACGTttat                                                                       <  1:2660821/148‑1 (MQ=255)
                                                                             gggCGTGAAAACTTAATGCAGGCCGGGGCGATCACCATGTTGACCGTGCGTCTGGGGTCGGTGATTTCTCCCATGATTGGCGGTTTATTGCTGGCGACCGGTGGCGTAGCCTGGAACTACGGGCTGGCGGCGGCGGGCACGTttatta                                                                     <  2:1984610/148‑1 (MQ=255)
                                                                             gggCGTGAAAACTTAATGCAGGCCGGGGCGATCACCATGTTGACCGTGCGTCTGGGGTCGGTGATTTCTCCCATGATTGGCGGTTTATTGCTGGCGACCGGTGGCGTAGCCTGGAACTACGGGCTGGCGGCGGCGGGCACGTttatta                                                                     >  1:2358958/1‑148 (MQ=255)
                                                                               gCGTGAAAACTTAATGCAGGCCGGGGCGATCACCATGTTGACCGTGCGTCTGGGGTCGGTGATTTCGCCCATGATTGGCGGTTTATTGCTGGCGACCGGTGGCGTAGCCTGGAACTACGGGCTGGCGGCGGCGGGCACGTTTATTAcc                                                                   <  2:1038104/148‑1 (MQ=255)
                                                                               gCGTGAAAACTTAATGCAGGCCGGGGCGATCACCATGTTGACCGTGCGTCTGGGGTCGGTGATTTCGCCCATGATTGGCGGTTTATTGCTGGCGACCGGTGGCGTAGCCTGGAACTACGGGCTGGCGGCGGCGGGCACGTTTATTAcc                                                                   <  2:2809982/148‑1 (MQ=255)
                                                                                   gAAAACTTAATGCAGGCCGGGGCGATCACCATGTTGACCGTGCGTCTGGGGTCGGTGATTTCTCCCATGATTGGCGGTTTATTGCTGGCGACCGGTGGCGTAGCCTGGAACTACGGGCTGGCGGCGGCGGGCACGTTTATTACCTTGc                                                               <  1:3191719/148‑1 (MQ=255)
                                                                                   gAAAACTTAATGCAGGCCGGGGCGATCACCATGTTGACCGTGCGTCTGGGGTCGGTGATTTCTCCCATGATTGGCGGTTTATTGCTGGCGACCGGTGGCGTAGCCTGGAACTACGGGCTGGCGGCGGCGGGCACGTTTATTACCTTGc                                                               <  1:3191419/148‑1 (MQ=255)
                                                                                        cTTAATGCAGGCCGGGGCGATCACCATGTTGACCGTGCGTCTGGGGTCGGTGATTTCTCCCATGATTGGCGGTTTATTGCTGGCGACCGGTGGCGTAGCCTGGAACTACGGGCTGGCGGCGGCGGGCACGTTTATTACCTTGCTACCg                                                          >  1:2587031/1‑148 (MQ=255)
                                                                                        cTTAATGCAGGCCGGGGCGATCACCATGTTGACCGTGCGTCTGGGGTCGGTGATTTCTCCCATGATTGGCGGTTTATTGCTGGCGACCGGTGGCGTAGCCTGGAACTACGGGCTGGCGGCGGCGGGCACGTTTATTACCTTGCTACCg                                                          >  1:2586660/1‑148 (MQ=255)
                                                                                          tAATGCAGGCCGGGGCGATCACCATGTTGACCGTGCGTCTGGGGTCGGTGATTTCGCCCATGATTGGCGGTTTATTGCTGGCGACCGGTGGCGTAGCCTGGAACTACGGGCTGGCGGCGGCGGGCACGTTTATTACCTTGCTACCgtt                                                        >  2:36710/1‑148 (MQ=255)
                                                                                              gCAGGCCGGGGCGATCACCATGTTGACCGTGCGTCTGGGGTCGGTGATTTCTCCCATGATTGGGGGTTTATTGCTGGCGACCGGTGGCGTAGCCTGGAACTACGGGCTGGCGGCGGCGGGCACGTTTATTACCTTGCTACCGTTGTTa                                                    >  1:2108624/1‑148 (MQ=255)
                                                                                              gCAGGCCGGGGCGATCACCATGTTGACCGTGCGTCTGGGGTCGGTGATTTCTCCCATGATTGGCGGTTTATTGCTGGCGACCGGTGGCGTAGCCTGGAACTACGGGCTGGCGGCGGCGGGCACGTTTATTACCTTGCTACCGTTGTTa                                                    <  2:1077130/148‑1 (MQ=255)
                                                                                              gCAGGCCGGGGCGATCACCATGTTGACCGTGCGTCTGGGGTCGGTGATTTCTCCCATGATTGGCGGTTTATTGCTGGCGACCGGTGGCGTAGCCTGGAACTACGGGCTGGCGGCGGCGGGCACGTTTATTACCTTGCTACCGTTGTTa                                                    <  2:1077044/148‑1 (MQ=255)
                                                                                              gCAGGCCGGGGCGATCACCATGTTGACCGTGCGTCTGGGGTCGGTGATTTCTCCCATGATTGGCGGTTTATTGCTGGCGACCGGTGGCGTAGCCTGGAACTACGGGCTGGCGGCGGCGGGCACGTTTATTACCTTGCTACCGTTGTTa                                                    <  1:1413091/148‑1 (MQ=255)
                                                                                               cAGGCCGGGGCGATCACCATGTTGACCGTGCGTCTGGGGTCGGTGATTTCTCCCATGATTGGCGGTTTATTGCTGGCGACCGGTGGCGTAGCCTGGAACTACGGGCTGGCGGCGGCGGGCACGTTTATTACCTTGTTACCGTTGTTaa                                                   >  2:516385/1‑148 (MQ=255)
                                                                                                aGGCCGGGGCGATCACCATGTTGACCGTGCGTCTGGGGTCGGTGATTTCGCCCATGATTGGCGGTTTATTGCTGGCGACCGGTGGCGTAGCCTGGAACTACGGGCTGGCGGCGGCGGGCACGTTTATTACCTTGCTACCGTTGTTAAg                                                  <  1:605088/148‑1 (MQ=255)
                                                                                                 ggCCGGGGCGATCACCATGTTGACCGTGCGTCTGGGGTCGGTGATTTCGCCCATGATTGGCGGTTTATTGCTGGCGACCGGTGGCGTAGCCTGGAACTACGGGCTGGCGGCGGCGGGCACGTTTATTACCTTGCTACCGTTGTTAAGc                                                 >  1:1031114/1‑148 (MQ=255)
                                                                                                   ccGGGGCGATCACCATGTTGACCGTGCGTCTGGGGTCGGTGATTTCTCCCATGATTGGCGGTTTATTGCTGGCGACCGGTGGCGTAGCCTGGAACTACGGGCTGGCGGCGGCGGGCACGTTTATTACCTTGCTACCGTTGTTAAGCCt                                               <  1:408462/148‑1 (MQ=255)
                                                                                                     ggggCGATCACCATGTTGACCGTGCGTCTGGGGTCGGTGATTTCTCCCATGATTGGCGGTTTATTGCTGGCGACCGGTGGCGTAGCCTGGAACTACGGGCTGGCGGCGGCGGGCACGTTTATTACCTTGCTACCGTTGTTAAGCCTTc                                             <  1:2949495/148‑1 (MQ=255)
                                                                                                     ggggCGATCACCATGTTGACCGTGCGTCTGGGGTCGGTGATTTCTCCCATGATTGGCGGTTTATTGCTGGCGACCGGTGGCGTAGCCTGGAACTACGGGCTGGCGGCGGCGGGCACGTTTATTACCTTGCTACCGTTGTTAAGCCTTc                                             <  2:618005/148‑1 (MQ=255)
                                                                                                       ggCGATCACCATGTTGACCGTGCGTCTGGGGTCGGTGATTTCTCCCATGATTGGCGGTTTATTGCTGGCGACCGGTGGCGTAGCCTGGAACTACGGGCTGGCGGCGGCGGGCACGTTTATTACCTTGCTACCGTTGTTAAGCCTTCCg                                           >  1:1908237/1‑148 (MQ=255)
                                                                                                         cGATCACCATGTTGACCGTGCGTCTGGGGTCGGTGATTTCGCCCATGAGTGGCGGTTTATTGCTGGCGACCGGTGGCGTAGCCTGGAACTACGGGCTGGCGGCGGCGGGCACGTGTATTACCTTGCTACCGTTGTTAAGCCTTCCGGc                                         >  2:87801/1‑148 (MQ=255)
                                                                                                          gATCACCATGTTGACCGTGCGTCTGGGGTCGGTGATTTCGCCCATGATTGGCGGTTTATTGCTGGCGACCGGTGGCGTAGCCTGGAACTACGGGCTGGCGGCGGCGGGCACGTTTATTACCTTGCTACCGTTGTTAAGCCTTCCGGCg                                        <  1:356896/148‑1 (MQ=255)
                                                                                                           aTCACCATGTTGACCGTGCGTCTGGGGTCGGTGATTTCTCCCATGATTGGCGGTTTATTGCTGGCGACCGGTGGCGTAGCCTGGAACTACGGGCTGGCGGCGGCGGGCACGTTTATTACCTTGCTACCGTTGTTAAGCCTTCCGGCGt                                       <  1:3119506/148‑1 (MQ=255)
                                                                                                            tCACCATGTTGACCGTGCGTCTGGGGTCGGTGATTTCTCCCATGATTGGCGGTTTATTGCTGGCGACCGGTGGCGTAGCCTGGAACTACGGGCTGGCGGCGGCGGGCACGTTTATTACCTTGCTACCGTTGTTAAGCCTTCCGGCGtt                                      >  2:2354113/1‑148 (MQ=255)
                                                                                                             cACCATGTTGACCGTGCGTCTGGGGTCGGTGATTTCTCCCATGATTGGCGGTTTATTGCTGGCGACCGGTGGCGTAGCCTGGAACTACGGGCTGGCGGCGGCGGGCACGTTTATTACCTTGCTACCGTTGTTAAGCCTTCCGGCGTTg                                     >  2:2625730/1‑148 (MQ=255)
                                                                                                             cACCATGTTGACCGTGCGTCTGGGGTCGGTGATTTCGCCCATGATTGGCGGTTTATTGCTGGCGACCGGTGGCGTAGCCTGGAACTACGGGCTGGCGGCGGCGGGCACGTTTATTACCTTGCTACCGTTGTTAAGCCTTCCGGCGTTg                                     <  1:41876/148‑1 (MQ=255)
                                                                                                               ccctgtgGACCGTGCGTCTGGGGTCGGTGATTTCGCCCATGATTGGCGGTTTATTGCTGGCGACCGGTGGCGTAGCCTGGAACTACGGGCTGGCGGCGGCGGGCACGTTTATTACCTTGCTACCGTTGTTAAGCCTTCCGGCGTTgcc                                   <  2:3160637/145‑1 (MQ=255)
                                                                                                                cATGTTGACCGTGCGTCTGGGGTCGGTGATTTCTCCCATGATTGGCGGTTTATTGCTGGCGACCGGTGGCGTAGCCTGGAACTACGGGCTGGCGGCGGCGGGCACGTTTATTACCTTGCTACCGTTGTTAAGCCTTCCGGCGTTgcca                                  >  1:2318210/1‑148 (MQ=255)
                                                                                                                 aTGTTGACCGTGCGTCTGGGGTCGGTGATTTCTCCCATGATTGGCGGTTTATTTCTGGCGACCGGTGGCGTAGCCTGGAACTACGGGCTGGCGGCGGCGGGCACGTTTATTACCTTGCTACCGTTGTTAAGCCTTACGGCGTTgccac                                 >  1:1569052/1‑148 (MQ=255)
                                                                                                                    ttGACCGTGCGTCTGGGGTCGGTGATTTCTCCCATGATTGGCGGTTTATTGCTGGCGACCGGTGGCGTAGCCTGGAACTACGGGCTGGCGGCGGCGGGCACGTTTATTACCTTGCTACCGTTGTTAAGCCTTCCGGCGTTgccaccgc                              <  2:243463/148‑1 (MQ=255)
                                                                                                                    ttGACCGTGCGTCTGGGGTCGGTGATTTCTCCCATGATTGGCGGTTTATTGCTGGCGACCGGTGGCGTAGCCTGGAACTACGGGCTGGCGGCGGCGGGCACGTTTATTACCTTGCTACCGTTGTTAAGCCTTCCGGCGTTgccaccgc                              <  1:2769760/148‑1 (MQ=255)
                                                                                                                    ttGACCGTGCGTCTGGGGTCGGTGATTTCTCCCATGATTGGCGGTTTATTGCTGGCGACCGGTGGCGTAGCCTGGAACTACGGGCTGGCGGCGGCGGGCACGTTTACTACCTTGCTACCGTTGTTAAGCCTTCCGGCGTTgccaccgc                              <  2:243459/148‑1 (MQ=255)
                                                                                                                    ttGACCGTGCGTCTGGGGGCGGTGATTTCTCCCATGATTGGCGGTTTATTGCTGGCGACCGGTGGCGTAGCCTGGAACTACGGGCTGGCGGCGGCGGGCACGTTTATTACCTTGCTACCGTTGTTAAGCCTTCCGGCGTTgccaccgc                              <  2:242976/148‑1 (MQ=255)
                                                                                                                           tGCGTCTGGGGTCGGTGATTTCGCCCATGATTGGCGGTTTATTGCTGGCGACCGGTGGCGTAGCCTGGAACTACGGGCTGGCGGCGGCGGGCACGTTTATTACCTTGCTACCGTTGTTAAGCCTTCCGGCGTTGCCACCGCCACCGCa                       >  2:2868979/1‑148 (MQ=255)
                                                                                                                             cGTCTGGGGTCGGTGATTTCTCCCATGATTGGCGGTTTATTGCTGGCGACCGGTGGCGTAGCCTGGAACTACGGGCTGGCGGCGGCGGGCACGTTTATTACCTTGCTACCGTTGTTAAGCCTTCCGGCGTTGCCACCGCCACCGCAGc                     >  2:1734876/1‑148 (MQ=255)
                                                                                                                             cGTCTGGGGTCGGTGATTTCTCCCATGATTGGCGGTTTATTGCTGGCGACCGGTGGCGTAGCCTGGAACTACGGGCTGGCGGCGGCGGGCACGTTTATTACCTTGCTACCGTTGTTAAGCCTTCCGGCGTTGCCACCGCCACCGCAGc                     <  1:2870503/148‑1 (MQ=255)
                                                                                                                             cGTCTGGGGTCGGTGATTTCGCCCATGATTGGCGGTTTATTGCTGGCGACCGGTGGCGTAGCCTGGAACTACGGGCTGGCGGCGGCGGGCACGTTTATTACCTTGCTACCGTTGTTAAGCCTTCCGGCGTTGCCACCGCCACCGCAGc                     >  2:1308605/1‑148 (MQ=255)
                                                                                                                                cTGGGGTCGGTGATTTCTCCCATGATTGGCGGTTTATTGCTGGCGACCGGTGGCGTAGCCTGGAACTACGGGCTGGCGGCGGCGGGCACGTTTATTACCTTGCTACCGTTGTTAAGCCTTCCGGCGTTGCCACCGCCACCGCAGCcgc                  <  2:2212083/148‑1 (MQ=255)
                                                                                                                                      tCGGTGATTTCGCCCATGATTGGCGGTGTATTGCTGGCGACCGGTGGCGTAGCCTGGAACTACGGGCTGGCGGCGGCGGGCACGTTTATTACCTTGCTACCGTTGTTAAGCCTTCCGGCGTTGCCACCGCCACCGCAGCCGCGTGAGc            <  2:1698194/148‑1 (MQ=255)
                                                                                                                                         gTGATTTCGCCCATGATTGGCGGTTTATTGCTGGCGACCGGTGGCGTAGCCTGGAACTACGGGCTGGCGGCGGCGGGCACGTTTATTACCTTGCTACCGTTGTTAAGCCTTCCGGCGTTGCCACCGCCACCGCAGCCGCGTGAGCATc         >  1:1865256/1‑148 (MQ=255)
                                                                                                                                          tGATTTCTCCCATGATTGGCGGTTTATTGCTGGCGACCGGTGGCGTAGCCTGGAACTACGGGCTGGCGGCGGCGGGCACGTTTATTACCTTGCTACCGTTGTTAAGCCTTCCGGCGTTGCCACCGCCACCGCAGCCGCGTGAGCATcc        >  1:1785594/1‑148 (MQ=255)
                                                                                                                                          tGATTTCGCCCATGATTGGCGGTTTATTGCTGGCGACCGGTGGCGTAGCCTGGAACTACGGGCTGGCGGCGGCGGGCACGTTTATTACCTTGCTACCGTTGTTAAGCCTTCCGGCGTTGCCACCGCCACCGCAGCCGCGTGAGCATcc        >  1:2281374/1‑148 (MQ=255)
                                                                                                                                          tGATTTCGCCCATGATTGGCGGTTTATTGCTGGCGACCGGTGGCGTAGCCTGGAACTACGGGCTGGCGGCGGCGGGCACGTTTATTACCTTGCTACCGTTGTTAAGCCTTCCGGCGTTGCCACCGCCACCGCAGCCGCGTGAGCATcc        >  1:2280831/1‑148 (MQ=255)
                                                                                                                                            aTTTCGCCCATGATTGGCGGTTTATTGCTGGCGACCGGTGGCGTAGCCTGGAACTACGGGCTGGCGGCGGCGGGCACGTTTATTACCTTGCTACCGTTGTTAAGCCTTCCGGCGTTGCCACCGCCACCGCAGCCGCGTGAGCATCCGt      <  1:1147349/148‑1 (MQ=255)
                                                                                                                                            aTTTCGCCCATGATTGGCGGTTTATTGCTGGCGACCGGTGGCGTAGCCTGGAACTACGGGCTGGCGGCGGCGGGCACGTTTATTACCTTGCTACCGTTGTTAAGCCTTCCGGCGTTGCCACCGCCACCGCAGCCGCGTGAGCATCCGt      >  1:3470958/1‑148 (MQ=255)
                                                                                                                                                cGCCCATGATTGGCGGTTTATTGCTGGCGACCGGTGGCGTAGCCTGGAACTACGGGCTGGCGGCGGCGGGCACGTTTATTACCTTGCTACCGTTGTTAAGCCTTCCGGCGTTGCCACCGCCACCGCAGCCGCGTGAGCATCCGTTGaa  <  1:3229467/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                  
ATTTACTTGGTTTATGGGATGGTTTTTTCGCATCGCTTGGCGTTACGGCGCTATTGGCGGCGACACCAGCACTGGTAGGGCGTGAAAACTTAATGCAGGCCGGGGCGATCACCATGTTGACCGTGCGTCTGGGGTCGGTGATTTCGCCCATGATTGGCGGTTTATTGCTGGCGACCGGTGGCGTAGCCTGGAACTACGGGCTGGCGGCGGCGGGCACGTTTATTACCTTGCTACCGTTGTTAAGCCTTCCGGCGTTGCCACCGCCACCGCAGCCGCGTGAGCATCCGTTGAA  >  CP009273/618096‑618387

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A