Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F368 I0 R1 1472 214.4 7116750 95.9% 6824963 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,769,763 T→A 100% intergenic (+62/‑81) aroD → / → ydiF 3‑dehydroquinate dehydratase/putative acetyl‑CoA:acetoacetyl‑CoA transferase: alpha subunit/beta subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,769,7630TA97.6% 688.9 / 4.4 212intergenic (+62/‑81)aroD/ydiF3‑dehydroquinate dehydratase/putative acetyl‑CoA:acetoacetyl‑CoA transferase: alpha subunit/beta subunit
Reads supporting (aligned to +/- strand):  ref base T (3/2);  new base A (123/84);  total (126/86)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.89e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 216 total aligned reads displayed.

GTGCGGTAAAAAAAGCGTCTGCGCCAGGGCAAATCTCGGTAAATGATTTGCGCACGGTATTAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATT‑TAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAACCACCTCGTATTAATGGACGAGTGCCGGTCCTGTCGGCACAGGAAGCG  >  CP009273/1769619‑1769906
                                                                                                                                                 |                                                                                                                                               
gTGCGGTAAAAAAAGCGTCTGCGCCAGGGCAAATCTCGGTAAATGATTTGCGCACGGTATTAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATT‑aaat                                                                                                                                              <  2:2582521/148‑1 (MQ=255)
   cGGTAAAAAAAGCGTCTGCGCCAGGGCAAATCTCGGTAAATGATTTGCGCACGGTATTAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATt‑aaataaa                                                                                                                                           <  1:1906963/148‑1 (MQ=255)
     gTAAAAAAAGCGTCTGCGCCAGGGCAAATCTCGGTAAATGATTTGCGCACGGTATTAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATT‑AAATaaaaa                                                                                                                                         >  1:3188470/1‑148 (MQ=255)
      tAAAAAAAGCGTCTGCGCCAGGGCAAATCTCGGTAAATGATTTGCGCCCGGTATTAACTATTTTACACCAGGCATAAGCATTAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATT‑AAATAAAAat                                                                                                                                        <  2:3259999/148‑1 (MQ=255)
        aaaaaaGCGTCTGCGCCAGGGCAAATCTCGGTAAATGATTTGCGCACGGTATTAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATT‑AAATAAAAatat                                                                                                                                      <  2:3404904/148‑1 (MQ=255)
         aaaaaGCGTCTGCGCCAGGGCAAATCTCGGTAAATGATTTGCGCACGGTATTAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATT‑AAATAAAAATAtt                                                                                                                                     <  2:1116139/148‑1 (MQ=255)
         aaaaaGCGTCTGCGCCAGGGCAAATCTCGGTAAATGATTTGCGCACGGTATTAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATT‑AAATAAAAATAtt                                                                                                                                     >  1:1663460/1‑148 (MQ=255)
          aaaaGCGTCTGCGCCAGGGCAAATCTCGGTAAATGATTTGCGCACGGTATTAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATT‑AAATAAAAATAttt                                                                                                                                    <  1:439412/148‑1 (MQ=255)
           aaaGCGTCTGCGCCAGGGCAAATCTCGGTAAATGATTTGCGCACGGTATTAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATT‑AAATAAAAATATTTc                                                                                                                                   <  1:1466433/148‑1 (MQ=255)
              gCGTCTGCGCCAGGGCAAATCTCGGTAAATGATTTGCGCACGGTATTAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATT‑AAATAAAAATATTTCGTg                                                                                                                                >  2:3082699/1‑148 (MQ=255)
              gCGTCTGCGCCAGGGCAAATCTCGGTAAATGATTTGCGCACGGTATTAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATT‑AAATAAAAATATTTCGTg                                                                                                                                <  2:2427687/148‑1 (MQ=255)
                 tCTGCGCCAGGGCAAATCTCGGTAAATGATTTGCGCACGGTATTAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATT‑AAATAAAAATATTTCGTGAGt                                                                                                                             <  1:1042406/148‑1 (MQ=255)
                 tCTGCGCCAGGGCAAATCTCGGTAAATGATTTGCGCACGGTATTAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATT‑AAATAAAAATATTTCGTGAGt                                                                                                                             >  1:1925173/1‑148 (MQ=255)
                  cTGCGCCAGGGCAAATCTCGGTAAATGATTTGCGCACGGTATTAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATT‑AAATAAAAATATTTCGTGAGtc                                                                                                                            >  2:1616100/1‑148 (MQ=255)
                   tGCGCCAGGGCAAATCTCGGTAAATGATTTGCGCACGGTATTAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATT‑AAATAAAAATATTTCGTGAGtct                                                                                                                           <  2:896163/148‑1 (MQ=255)
                         aGGGCAAATCTCGGTAAATGATTTGCGCACGGTATTAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATT‑AAATAAAAATATTTCGTGAGTCTCTGTgc                                                                                                                     >  2:2569855/1‑148 (MQ=255)
                         aGGGCAAATCTCGGTAAATGATTTGCGCACGGTATTAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATT‑AAATAAAAATATTTCGTGAGTCTCTGTgc                                                                                                                     >  2:3362112/1‑148 (MQ=255)
                          gggCAAATCTCGGTAAATGATTTGCGCACGGTATTAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATT‑AAATAAAAATATTTCGTGAGTCTCTGTgcg                                                                                                                    <  1:1226859/148‑1 (MQ=255)
                           ggCAAATCTCGGTAAATGATTTGCGCACGGTATTAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATT‑AAATAAAAATATTTCGTGAGTCTCTGTgcgc                                                                                                                   >  1:2668703/1‑148 (MQ=255)
                            gCAAATCTCGGTAAATGATTTGCGCACGGTATTAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATT‑AAATAAAAATATTTCGTGAGTCTCTGTGCGCt                                                                                                                  >  2:2734725/1‑148 (MQ=255)
                             cAAATCTCGGTAAATGATTTGCGCACGGTATTAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATT‑TAATAAAAATATTTCGTGAGTCTCTGTGCGCTa                                                                                                                 <  2:2666703/148‑1 (MQ=255)
                             cAAATCTCGGTAAATGATTTGCGCACGGTATTAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATT‑TAATAAAAATATTTCGTGAGTCTCTGTGCGCTa                                                                                                                 <  2:3242543/148‑1 (MQ=255)
                              aaaTCTCGGTAAATGATTTGCGCACGGTATTAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATT‑AAATAAAAATATTTCGTGAGTCTCTGTGCGCTaa                                                                                                                <  2:1407179/148‑1 (MQ=255)
                               aaTCTCGGTAAATGATTTGCGCACGGTATTAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATT‑AAATAAAAATATTTCGTGAGTCTCTGTGCGCTAAt                                                                                                               <  1:2664272/148‑1 (MQ=255)
                                agctcGGTAAATGATTTGCGCACGGTATTAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATT‑AAATAAAAATATTTCGTGAGTCTCTGTGCGCTAAtt                                                                                                              <  2:1999399/146‑1 (MQ=255)
                                aTCTCGGTAAATGATTTGCGCACGGTATTAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATT‑ACATAAAAATATTTCGTGAGTCTCTGTGCGCTAAtt                                                                                                              >  1:2431120/1‑148 (MQ=255)
                                aTCTCGGTAAATGATTTGCGCACGGTATTAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATT‑AAATAAAAATATTTCGTGAGTCTCTGTGCGCTAAtt                                                                                                              >  1:1161243/1‑148 (MQ=255)
                                aTCTCGGTAAATGATTTGCGCACGGTATTAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATT‑AAATAAAAATATTTCGTGAGTCTCTGTGCGCTAAtt                                                                                                              >  1:2079865/1‑148 (MQ=255)
                                    cGGTAAATGATTTGCGCACGGTATTAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATT‑AAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTcc                                                                                                          >  1:3419246/1‑148 (MQ=255)
                                       tAAATGATTTGCGCACGGTATTAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATT‑AAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCAtt                                                                                                       <  2:316430/148‑1 (MQ=255)
                                        aaaTGATTTGCGCACGGTATTAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATT‑AAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCAttt                                                                                                      <  1:2501904/148‑1 (MQ=255)
                                         aaTGATTTGCGCACGGTATTAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATT‑AAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTg                                                                                                     >  2:471081/1‑148 (MQ=255)
                                              tttGCGCACGGTATTAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATT‑AAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTa                                                                                                >  2:1851821/1‑148 (MQ=255)
                                                     aCGGTATTAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATT‑AAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGaaaa                                                                                         <  2:895310/148‑1 (MQ=255)
                                                      cGGTATTAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATT‑AAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAAt                                                                                        >  2:542897/1‑148 (MQ=255)
                                                       ggTATTAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATT‑AAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATc                                                                                       >  2:928094/1‑148 (MQ=255)
                                                       ggTATTAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATT‑AAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATc                                                                                       >  2:74226/1‑148 (MQ=255)
                                                         tATTAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATT‑AAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATcac                                                                                     >  1:685435/1‑148 (MQ=255)
                                                           ttAACTATATTACACCAGGCATAAGCAATAATAGTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATT‑AAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACAt                                                                                   <  2:3522072/148‑1 (MQ=255)
                                                            tAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATT‑AAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATc                                                                                  <  2:1289772/148‑1 (MQ=255)
                                                            tAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATT‑AAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATc                                                                                  >  2:588578/1‑148 (MQ=255)
                                                              aCTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATT‑AAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCGATTTGGCGTAGGGAAAATCACATCTg                                                                                >  2:1312684/1‑148 (MQ=255)
                                                              aCTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATT‑AAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTCGGGAAAATCACATCTg                                                                                >  2:2830514/1‑148 (MQ=255)
                                                                tATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATT‑AAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGaa                                                                              >  2:1024911/1‑148 (MQ=255)
                                                                  ttttACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATT‑AAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATc                                                                            >  1:1701253/1‑148 (MQ=255)
                                                                   tttACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATT‑AAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCa                                                                           >  1:1849713/1‑148 (MQ=255)
                                                                      acacCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATT‑AAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGa                                                                        <  1:510099/148‑1 (MQ=255)
                                                                         ccAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATT‑AAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAAtt                                                                     <  1:1430232/148‑1 (MQ=255)
                                                                         ccAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATT‑AAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAAtt                                                                     <  1:3082699/148‑1 (MQ=255)
                                                                           aGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATT‑AAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTaa                                                                   <  1:3022209/148‑1 (MQ=255)
                                                                           aGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATT‑AAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTaa                                                                   <  1:3022340/148‑1 (MQ=255)
                                                                              cATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATT‑AAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACaa                                                                >  2:773444/1‑148 (MQ=255)
                                                                              cATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATT‑AAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACaa                                                                <  1:317586/148‑1 (MQ=255)
                                                                                 aaGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATT‑AAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGa                                                             >  1:1359601/1‑148 (MQ=255)
                                                                                  aGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATT‑TAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGaa                                                            >  2:94070/1‑148 (MQ=255)
                                                                                       taataTTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATT‑AAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGAAACCTg                                                       >  1:2659356/1‑148 (MQ=255)
                                                                                         atatTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATT‑AAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGAAACCTGTa                                                     >  1:2708616/1‑148 (MQ=255)
                                                                                           atTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATT‑AAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCAGAATCAGGAATTAACAATGAAACCTGTaaa                                                   >  2:1567186/1‑148 (MQ=255)
                                                                                             ttCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATT‑AAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGAAACATGTAAAac                                                 >  2:2629808/1‑148 (MQ=255)
                                                                                                 gcggGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATT‑AAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAaccacc                                             <  2:1781718/148‑1 (MQ=255)
                                                                                                  cggGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATT‑AAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAACCACCt                                            <  1:313468/148‑1 (MQ=255)
                                                                                                     gAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATT‑AAATCAAACTATTTCTTGAGTCTCGGTGCGCTAATTCTCGATGTGGCGTCGGGACAATCACATCTGAATATGGAATTACGAATGCAGCCGGTAAAAGCACCTCGt                                         >  1:1855208/1‑148 (MQ=255)
                                                                                                     gAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATT‑AAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAACCACCTCGt                                         >  1:2154349/1‑148 (MQ=255)
                                                                                                        cacCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATT‑AAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAACCACCTCGTAtt                                      >  1:415251/1‑148 (MQ=255)
                                                                                                        cacCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATT‑AAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAACCACCTCGTAtt                                      >  1:2771316/1‑148 (MQ=255)
                                                                                                         acCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATT‑AAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAACCACCTCGTATTa                                     <  1:1891036/148‑1 (MQ=255)
                                                                                                         acCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATT‑AAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAACCACCTCGTATTa                                     <  1:1611990/148‑1 (MQ=255)
                                                                                                         acCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATT‑AAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAACCACCTCGTATTa                                     <  2:2193993/148‑1 (MQ=255)
                                                                                                         acCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATT‑AAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAACCACCTCGTATTa                                     <  2:1885930/148‑1 (MQ=255)
                                                                                                         acCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATT‑AAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAACCACCTCGTATTa                                     <  2:1663460/148‑1 (MQ=255)
                                                                                                         acCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATT‑AAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAACCACCTCGTATTa                                     <  2:3428230/148‑1 (MQ=255)
                                                                                                         acCCTCCCCGCAGAACTAAAAAATATATTCAATCGTATT‑AAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAACCACCTCGTATTa                                     <  2:2277462/148‑1 (MQ=255)
                                                                                                          cccTCCCCGCCGAACTAAAAAATATATTCAATCGTATT‑AAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAACCACCTCGTATTaa                                    <  2:1186/148‑1 (MQ=255)
                                                                                                           ccTCCCCGCCGAACTAAAAAATATATTCAATCGTATT‑AAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAACCACCTCGTATTAAt                                   >  2:1110864/1‑148 (MQ=255)
                                                                                                           ccTCCCCGCCGAACTAAAAAATATATTCAATCGTATT‑AAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAACCACCTCGTATTAAt                                   <  1:1684452/148‑1 (MQ=255)
                                                                                                           ccTCCCCGCCGAACTAAAAAATATATTCAATCGTATT‑AAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAACCACCTCGTATTAAt                                   <  1:3189030/148‑1 (MQ=255)
                                                                                                            cTCCCCGCCGAACTAAAAAATATATTCAATCGTATT‑AAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAACCACCTCGTATTAATg                                  >  2:1648357/1‑148 (MQ=255)
                                                                                                             tCCCCGCCGAACTAAAAAATATATTCAATCGTATT‑AAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAACCACCTCGTATTAATgg                                 <  1:933953/148‑1 (MQ=255)
                                                                                                             tCCCCGCCGAACTAAAAAATATATTCAATCGTATT‑AAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAACCACCTCGTATTAATgg                                 >  1:1958906/1‑148 (MQ=255)
                                                                                                              ccccGCCGAACTAAAAAATATATTCAATCGTATT‑AAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAACCACCTCGTATTAATGGa                                <  2:1161204/148‑1 (MQ=255)
                                                                                                               cccGCCGAACTAAAAAATATATTCAATCGTATT‑AAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATAAGGAATTAACAATGAAACCTGTCAAACCACCTCGTATTAATGGAc                               >  2:2976831/1‑148 (MQ=255)
                                                                                                               cccGCCGAACTAAAAAATATATTCAATCGTATT‑AAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATAAGGAATTAACAATGAAACCTGTAAAACCACCTCGTATTAATGGAc                               >  2:2976789/1‑148 (MQ=255)
                                                                                                                     gAACTAAAAAATATATTCAATCGTATT‑AAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAACCACCTCGTATTAATGGACGAGTGc                         >  1:1234179/1‑148 (MQ=255)
                                                                                                                      aaatAAAAAATATATTCAATCGTATTAAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAACCACCTCGTATTAATGGACGAGTGc                         <  1:550764/145‑1 (MQ=255)
                                                                                                                      aaCTAAAAAATATATTCAATCGTATT‑AAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAACCACCTCGTATTAATGGACGAGTGcc                        >  2:1716636/1‑148 (MQ=255)
                                                                                                                        cTAAAAAATATATTCAATCGTATT‑AAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAACCACCTCGTATTAATGGACGAGTGCCgg                      >  2:3138764/1‑148 (MQ=255)
                                                                                                                        cTAAAAAATATATTCAATCGTATT‑AAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAACCACCTCGTATTAATGGACGAGTGCCgg                      >  2:195445/1‑148 (MQ=255)
                                                                                                                        cTAAAAAATATATTCAATCGTATT‑AAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAACCACCTCGTATTAATGGACGAGTGCCgg                      >  2:1367504/1‑148 (MQ=255)
                                                                                                                         tAAAAAATATATTCAATCGTATT‑AAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAACCACCTCGTATTAATGGACGAGTGCCGGt                     >  2:3101980/1‑148 (MQ=255)
                                                                                                                           aaaaaTATATTCAATCGTATT‑AAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAACCACCTCGTATTAATGGACGAGTGCCGGTcc                   <  1:1110864/148‑1 (MQ=255)
                                                                                                                             aaaTATATTCAATCGTATT‑AAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAACCACCTCGTATTAATGGACGAGTGCCGGTCCTg                 <  2:366111/148‑1 (MQ=255)
                                                                                                                               atatatTCAATCGTATT‑AAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAACCACCTCGTATTAATGGACGAGTGCCGGTCCTGTc               >  1:83486/1‑148 (MQ=255)
                                                                                                                               atatatTCAATCGTATT‑AAATAAAAATATTTCGTGAGTCTATGTGCGCTAATTCTCCATTTGTCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAACCACCTCGAATTAATGGACGAGTGCCGGTCCTGTc               >  1:83861/1‑148 (MQ=255)
                                                                                                                                 atatTCAATCGTATT‑AAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAACCACCTCGTATTAATGGACGAGTGCCGGTCCTGTCgg             >  2:368788/1‑148 (MQ=255)
                                                                                                                                   atTCAATCGTATT‑AAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAACCACCTCGTATTAATGGACGAGTGCCGGTCCTGTCGGca           >  1:343924/1‑148 (MQ=255)
                                                                                                                                        aTCGTATT‑AAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAACCACCTCGTATTAATGGACGAGTGCCGGTCCTGTCGGCACAGGa      <  1:2885739/148‑1 (MQ=255)
                                                                                                                                          cGTATT‑AAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAACCGCCTCGTATTAATGGACGAGTGCCGGTCCTGTCGGCACAGGAAg    >  1:2191534/1‑148 (MQ=255)
                                                                                                                                          cGTATT‑AAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAACCACCTCGTATTAATGGACGAGTGCCGGTCCTGTCGGCACAGGAAg    >  2:2801954/1‑148 (MQ=255)
                                                                                                                                           gTATT‑AAATAAAAATATTCCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAACCACCTCGTATTAATGGACGAGTGCCGGTCCTGTCGGCACAGGAAGc   <  1:1185085/148‑1 (MQ=255)
                                                                                                                                            tATT‑AAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCGCATCTGAATCAGGAATTAACGATGAAACCTGTAAAACCACCTCGTATTAATGGACGAGTGCCGGTCCTGTCGGCACAGGAAGCg  >  2:1181929/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                               
GTGCGGTAAAAAAAGCGTCTGCGCCAGGGCAAATCTCGGTAAATGATTTGCGCACGGTATTAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATT‑TAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAACCACCTCGTATTAATGGACGAGTGCCGGTCCTGTCGGCACAGGAAGCG  >  CP009273/1769619‑1769906

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TTTTGGTGCGGTAAAAAAAGCGTCTGCGCCAGGGCAAATCTCGGTAAATGATTTGCGCACGGTATTAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATT‑TAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAACCACCTCGTATTAATGGACGAGTGCCGGTCCTGTCGGCACAGGAAGCGGTGAAT  >  CP009273/1769614‑1769912
                                                                                                                                                      |                                                                                                                                                     
TTTTGGTGCGGTAAAAAAAGCGTCTGCGCCAGGGCAAATCTCGGTAAATGATTTGCGCACGGTATTAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATT‑A                                                                                                                                                       <  SRR19973067.2190554/150‑1 (MQ=60)
        CGGTAAAAAAAGCGTCTGCGCCAGGGCAAATCTCGGTAAATGATTTGCGCACGGTATTAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATT‑AAATAAAAA                                                                                                                                               >  SRR19973067.3208619/1‑150 (MQ=60)
        CGGTAAAAAAAGCGTCTGCGCCAGGGCAAATCTCGGTAAATGATTTGCGCACGGTATTAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATT‑AAATAAAAA                                                                                                                                               <  SRR19973067.1929479/150‑1 (MQ=60)
        CGGTAAAAAAAGCGTCTGCGCCAGGGCAAATCTCGGTAAATGATTTGCGCACGGTATTAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATT‑AAATAAAAA                                                                                                                                               <  SRR19973067.1919966/150‑1 (MQ=60)
         GGTAAAAAAAGCGTCTGCGCCAGGGCAAATCTCGGTAAATGATTTGCGCACGGTATTAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATT‑AAATAAAAAT                                                                                                                                              <  SRR19973067.43934/150‑1 (MQ=60)
          GTAAAAAAAGCGTCTGCGCCAGGGCAAATCTCGGTAAATGATTTGCGCACGGTATTAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATT‑AAATAAAAATA                                                                                                                                             >  SRR19973067.1995619/1‑150 (MQ=60)
            AAAAAAAGCGTCTGCGCCAGGGCAAATCTCGGTAAATGATTTGCGCACGGTATTAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATT‑AAATAAAAATATT                                                                                                                                           >  SRR19973067.3450045/1‑150 (MQ=60)
            AAAAAAAGCGTCTGCGCCAGGGCAAATCTCGGTAAATGATTTGCGCACGGTATTAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATT‑AAATAAAAATATT                                                                                                                                           >  SRR19973067.1674930/1‑150 (MQ=60)
            AAAAAAAGCGTCTGCGCCAGGGCAAATCTCGGTAAATGATTTGCGCACGGTATTAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATT‑AAATAAAAATATT                                                                                                                                           >  SRR19973067.3129088/1‑150 (MQ=60)
               AAAAGCGTCTGCGCCAGGGCAAATCTCGGTAAATGATTTGCGCACGGTATTAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATT‑AAATAAAAATATTTCG                                                                                                                                        <  SRR19973067.442467/150‑1 (MQ=60)
                AAAGCGTCTGCGCCAGGGCAAATCTCGGTAAATGATTTGCGCACGGTATTAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATT‑AAATAAAAATATTTCGT                                                                                                                                       >  SRR19973067.3446963/1‑150 (MQ=60)
                AAAGCGTCTGCGCCAGGGCAAATCTCGGTAAATGATTTGCGCACGGTATTAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATT‑AAATAAAAATATTTCGT                                                                                                                                       <  SRR19973067.1476217/150‑1 (MQ=60)
                   GCGTCTGCGCCAGGGCAAATCTCGGTAAATGATTTGCGCACGGTATTAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATT‑AAATAAAAATATTTCGTGAG                                                                                                                                    <  SRR19973067.1690078/150‑1 (MQ=60)
                    CGTCTGCGCCAGGGCAAATCTCGGTAAATGATTTGCGCACGGTATTAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATT‑AAATAAAAATATTTCGTGAGT                                                                                                                                   >  SRR19973067.1938309/1‑150 (MQ=60)
                      TCTGCGCCAGGGCAAATCTCGGTAAATGATTTGCGCACGGTATTAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATT‑AAATAAAAATATTTCGTGAGTCT                                                                                                                                 <  SRR19973067.1049571/150‑1 (MQ=60)
                        TGCGCCAGGGCAAATCTCGGTAAATGATTTGCGCACGGTATTAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATT‑AAATAAAAATATTTCGTGAGTCTCT                                                                                                                               <  SRR19973067.1985683/150‑1 (MQ=60)
                            CCAGGGCAAATCTCGGTAAATGATTTGCGCACGGTATTAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATT‑AAATAAAAATATTTCGTGAGTCTCTGTGC                                                                                                                           >  SRR19973067.138923/1‑150 (MQ=60)
                              AGGGCAAATCTCGGTAAATGATTTGCGCACGGTATTAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATT‑AAATAAAAATATTTCGTGAGTCTCTGTGCGC                                                                                                                         >  SRR19973067.2685842/1‑150 (MQ=60)
                              AGGGCAAATCTCGGTAAATGATTTGCGCACGGTATTAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATT‑AAATAAAAATATTTCGTGAGTCTCTGTGCGC                                                                                                                         >  SRR19973067.1955094/1‑150 (MQ=60)
                               GGGCAAATCTCGGTAAATGATTTGCGCACGGTATTAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATT‑AAATAAAAATATTTCGTGAGTCTCTGTGCGCT                                                                                                                        >  SRR19973067.529494/1‑150 (MQ=60)
                               GGGCAAATCTCGGTAAATGATTTGCGCACGGTATTAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATT‑AAATAAAAATATTTCGTGAGTCTCTGTGCGCT                                                                                                                        <  SRR19973067.1234297/150‑1 (MQ=60)
                               GGGCAAATCTCGGTAAATGATTTGCGCACGGTATTAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATT‑AAATAAAAATATTTCGTGAGTCTCTGTGCGCT                                                                                                                        <  SRR19973067.1235059/150‑1 (MQ=60)
                               GGGCAAATCTCGGTAAATGATTTGCGCACGGTATTAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATT‑AAATAAAAATATTTCGTGAGTCTCTGTGCGCT                                                                                                                        >  SRR19973067.2927775/1‑150 (MQ=60)
                                   AAATCTCGGTAAATGATTTGCGCACGGTATTAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATT‑ACATAAAAATATTTCGTGAGTCTCTGTGCGCTAATT                                                                                                                    >  SRR19973067.2447081/1‑150 (MQ=60)
                                   AAATCTCGGTAAATGATTTGCGCACGGTATTAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATT‑AAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATT                                                                                                                    >  SRR19973067.1168934/1‑150 (MQ=60)
                                   AAATCTCGGTAAATGATTTGCGCACGGTATTAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATT‑AAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATT                                                                                                                    >  SRR19973067.1168973/1‑150 (MQ=60)
                                   AAATCTCGGTAAATGATTTGCGCACGGTATTAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATT‑AAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATT                                                                                                                    >  SRR19973067.3344533/1‑150 (MQ=60)
                                   AAATCTCGGTAAATGATTTGCGCACGGTATTAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATT‑AAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATT                                                                                                                    >  SRR19973067.2093653/1‑150 (MQ=60)
                                   AAATCTCGGTAAATGATTTGCGCACGGTATTAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATT‑AAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATT                                                                                                                    >  SRR19973067.2447107/1‑150 (MQ=60)
                                    AATCTCGGTAAATGATTTGCGCACGGTATTAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATT‑AAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTC                                                                                                                   <  SRR19973067.2681399/150‑1 (MQ=60)
                                    AATCTCGGTAAATGATTTGCGCACGGTATTAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATT‑AAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTC                                                                                                                   <  SRR19973067.2681386/150‑1 (MQ=60)
                                       CTCGGTAAATGATTTGCGCACGGTATTAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATT‑AAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCC                                                                                                                >  SRR19973067.3441006/1‑150 (MQ=60)
                                        TCGGTAAATGATTTGCGCACGGTATTAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATT‑AAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCA                                                                                                               <  SRR19973067.1239444/150‑1 (MQ=60)
                                         CGGTAAATGATTTGCGCACGGTATTAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATT‑AAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCAT                                                                                                              <  SRR19973067.444333/150‑1 (MQ=60)
                                           GTAAATGATTTGCGCACTGTATTAACTATTTTACAACAGGCATAATCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATT‑AAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTT                                                                                                            <  SRR19973067.3058187/150‑1 (MQ=60)
                                           GTAAATGATTTGCGCACGGTATTAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATT‑AAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTT                                                                                                            <  SRR19973067.700473/150‑1 (MQ=60)
                                             AAATGATTTGCGCACGGTATTAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATT‑AAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGG                                                                                                          >  SRR19973067.62129/1‑150 (MQ=60)
                                             AAATGATTTGCGCACGGTATTAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATT‑AAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGG                                                                                                          <  SRR19973067.2518328/150‑1 (MQ=60)
                                                    TTGCGCACGGTATTAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATT‑AAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGG                                                                                                   >  SRR19973067.1906984/1‑150 (MQ=60)
                                                           CGGTATTAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATT‑AAATAAAAATATTTCGTGAGTCGCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCA                                                                                            <  SRR19973067.737583/150‑1 (MQ=60)
                                                            GGTATTAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATT‑AAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCAC                                                                                           >  SRR19973067.690147/1‑150 (MQ=60)
                                                                  AACTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTAAATCGTATT‑AAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGA                                                                                     <  SRR19973067.3049164/150‑1 (MQ=60)
                                                                   ACAATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATT‑AAATAAAAAAAAGTCGTGAGTCTCTGTGCGCTAATTATCAATTTGGCGTAGGGAAAATCACATCTGAA                                                                                    >  SRR19973067.188488/1‑150 (MQ=60)
                                                                     TATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATT‑AAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATC                                                                                  >  SRR19973067.1306646/1‑150 (MQ=60)
                                                                     TATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATT‑AAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATC                                                                                  >  SRR19973067.1712089/1‑150 (MQ=60)
                                                                     TATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATT‑AAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATC                                                                                  >  SRR19973067.1712943/1‑150 (MQ=60)
                                                                      ATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATT‑AAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCA                                                                                 >  SRR19973067.1862345/1‑150 (MQ=60)
                                                                      ATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATT‑AAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCA                                                                                 >  SRR19973067.1862324/1‑150 (MQ=60)
                                                                        TTTACACCAGGCATAAGCAATAATCTTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATT‑AAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGG                                                                               <  SRR19973067.2727936/150‑1 (MQ=60)
                                                                           ACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATT‑AAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAAT                                                                            <  SRR19973067.513574/150‑1 (MQ=60)
                                                                            CACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATT‑AAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATT                                                                           <  SRR19973067.178894/150‑1 (MQ=60)
                                                                             ACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATT‑AAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTA                                                                          <  SRR19973067.2124260/150‑1 (MQ=60)
                                                                              CCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCGATCGTATT‑AAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAA                                                                         >  SRR19973067.2707087/1‑150 (MQ=60)
                                                                              CCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATT‑AAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAA                                                                         <  SRR19973067.3102317/150‑1 (MQ=60)
                                                                              CCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATT‑AAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAA                                                                         <  SRR19973067.1439876/150‑1 (MQ=60)
                                                                                AGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATT‑AAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACA                                                                       <  SRR19973067.3041453/150‑1 (MQ=60)
                                                                                AGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATT‑AAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACA                                                                       <  SRR19973067.3041468/150‑1 (MQ=60)
                                                                                AGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATT‑AAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACA                                                                       <  SRR19973067.3041584/150‑1 (MQ=60)
                                                                                 GGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATT‑AAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAA                                                                      <  SRR19973067.1190139/150‑1 (MQ=60)
                                                                                   CATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATT‑AAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATG                                                                    <  SRR19973067.2360792/150‑1 (MQ=60)
                                                                                   CATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATT‑AAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATG                                                                    <  SRR19973067.319724/150‑1 (MQ=60)
                                                                                    ATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATT‑AAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGA                                                                   >  SRR19973067.1368887/1‑150 (MQ=60)
                                                                                         CAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATT‑AAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGAAACCT                                                              <  SRR19973067.2536427/150‑1 (MQ=60)
                                                                                          AATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATT‑AAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGAAACCTG                                                             >  SRR19973067.2676468/1‑150 (MQ=60)
                                                                                          AATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATT‑AAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGAAACCTG                                                             >  SRR19973067.2676558/1‑150 (MQ=60)
                                                                                            TAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATT‑AAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGAAACCTGTA                                                           >  SRR19973067.2726059/1‑150 (MQ=60)
                                                                                             AATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATT‑AAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAA                                                          >  SRR19973067.1427498/1‑150 (MQ=60)
                                                                                             AATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATT‑AAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCAGTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAA                                                          >  SRR19973067.3206914/1‑150 (MQ=60)
                                                                                                ATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATT‑AAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAAC                                                       <  SRR19973067.1862032/150‑1 (MQ=60)
                                                                                                      GCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATT‑AAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAACCACCTC                                                 >  SRR19973067.1453517/1‑150 (MQ=60)
                                                                                                       CGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATT‑AAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAACCACCTCG                                                <  SRR19973067.315545/150‑1 (MQ=60)
                                                                                                        GGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATT‑AAATCAAACTATTTCTTGAGTCTCGGTGCGCTAATTCTCGATGTGGCGTCGGGACAATCACATCTGAATATGGAATTACGAATGCAGCCGGTAAAAGCACCTCGT                                               >  SRR19973067.1867849/1‑150 (MQ=60)
                                                                                                        GGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATT‑AAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAACCACCTCGT                                               >  SRR19973067.2168751/1‑150 (MQ=60)
                                                                                                           AACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATT‑AAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAACCACCTCGTATT                                            >  SRR19973067.418134/1‑150 (MQ=60)
                                                                                                           AACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATT‑AAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAACCACCTCGTATT                                            >  SRR19973067.2789217/1‑150 (MQ=60)
                                                                                                             CACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATT‑AAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAACCACCTCGTATTAA                                          >  SRR19973067.3181513/1‑150 (MQ=60)
                                                                                                              ACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATT‑AAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAACCACCTCGTATTAAT                                         <  SRR19973067.1903941/150‑1 (MQ=60)
                                                                                                              ACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATT‑AAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAACCACCTCGTATTAAT                                         >  SRR19973067.2859979/1‑150 (MQ=60)
                                                                                                              ACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATT‑AAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAACCACCTCGTATTAAT                                         <  SRR19973067.1623077/150‑1 (MQ=60)
                                                                                                              ACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATT‑AAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAACCACCTCGTATTAAT                                         <  SRR19973067.1623059/150‑1 (MQ=60)
                                                                                                                CCTCCCCGCCGAACTAAAAAATATATTCAATCGTATT‑AAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAACCACCTCGTATTAATGG                                       >  SRR19973067.1972222/1‑150 (MQ=60)
                                                                                                                CCTCCCCGCCGAACTAAAAAATATATTCAATCGTATT‑AAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAACCACCTCGTATTAATGG                                       <  SRR19973067.3209150/150‑1 (MQ=60)
                                                                                                                CCTCCCCGCCGAACTAAAAAATATATTCAATCGTATT‑AAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAACCACCTCGTATTAATGG                                       <  SRR19973067.1696066/150‑1 (MQ=60)
                                                                                                                CCTCCCCGCCGAACTAAAAAATATATTCAATCGTATT‑AAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAACCACCTCGTATTAATGG                                       <  SRR19973067.1513408/150‑1 (MQ=60)
                                                                                                                CCTCCCCGCCGAACTAAAAAATATATTCAATCGTATT‑AAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAACCACCTCGTATTAATGG                                       <  SRR19973067.3209180/150‑1 (MQ=60)
                                                                                                                  TCCCCGCCGAACTAAAAAATATATTCAATCGTATT‑AAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAACCACCTCGTATTAATGGAC                                     <  SRR19973067.940287/150‑1 (MQ=60)
                                                                                                                       GCCGAACTAAAAAATATATTCAATCGTATT‑AAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAACCACCTCGTATTAATGGACGAGTG                                <  SRR19973067.2684573/150‑1 (MQ=60)
                                                                                                                        CCGAACTAAAAAATATATTCAATCGTATT‑AAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAACCACCTCGTATTAATGGACGAGTGC                               >  SRR19973067.1242455/1‑150 (MQ=60)
                                                                                                                           AAATAAAAAATATATTCAATCGTATTAAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAACCACCTCGTATTAATGGACGAGTGCCG                             <  SRR19973067.554393/150‑1 (MQ=60)
                                                                                                                                AAAAATATATTCAATCGTATT‑AAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAACCACCTCGTATTAATGGACGAGTGCCGGTCCTG                       <  SRR19973067.1118398/150‑1 (MQ=60)
                                                                                                                                  AAATATATTCAATCGTATT‑AAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAACCACCTCGTATTAATGGACGAGTGCCGGTCCTGTC                     >  SRR19973067.84780/1‑150 (MQ=60)
                                                                                                                                  AAATATATTCAATCGTATT‑AAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAACCACCTCGTATTAATGGACGAGTGCCGGTCCTGTC                     >  SRR19973067.84726/1‑150 (MQ=60)
                                                                                                                                  AAATATATTCAATCGTATT‑AAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAACCACCTCGTATTAATGGACGAGTGCCGGTCCTGTC                     >  SRR19973067.84398/1‑150 (MQ=60)
                                                                                                                                  AAATATATTCAATCGTATT‑AAATAAAAATATTTCGTGAGTCTATGTGCGCTAATTCTCCATTTGTCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAACCACCTCGAATTAATGGACGAGTGCCGGTCCTGTC                     >  SRR19973067.84777/1‑150 (MQ=60)
                                                                                                                                  AAATATATTCAATCGTATT‑AAATAAAAAAATTTATTGAGTCTGTGAGCGCTAATGCTCCATATGGGGAAGCGAAATTCAAATCTGAATCTGGAATTAACAATCAAACATGTAAAACAACCTCGAAAGAATGGACGAGTGAAGGTCCTGAC                     >  SRR19973067.1427706/1‑150 (MQ=23)
                                                                                                                                      ATATTCAATCGTATT‑AAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAACCACCTCGTATTAATGGACGAGTGCCGGTCCTGTCGGCA                 >  SRR19973067.346298/1‑150 (MQ=60)
                                                                                                                                       TATTCAATCGTATT‑AAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAACCACCTCGTATTAATGGACGAGTGCCGGTCCTGTCGGCAC                >  SRR19973067.370736/1‑150 (MQ=60)
                                                                                                                                       TATTCAATCGTATT‑AAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAACCACCTCGTATTAATGGACGAGTGCCGGTCCTGTCGGCAC                >  SRR19973067.2173541/1‑150 (MQ=60)
                                                                                                                                          TCAATCGTATT‑AAATAAAAATATTTCGTGAGTCTCTGTGCGCTAAGTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAACCACCTCGTATTAATGGACGAGTGCCGGTCCTGTCGGCACAGG             <  SRR19973067.1073299/150‑1 (MQ=60)
                                                                                                                                             ATCGTATT‑AAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAACCGCCTCGTATTAATGGACGAGTGCCGGTCCTGTCGGCACAGGAAG          >  SRR19973067.2206160/1‑150 (MQ=60)
                                                                                                                                             ATCGTATT‑AAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAACCACCTCGTATTAATGGACGAGTGCCGGTCCTGTCGGCACAGGAAG          <  SRR19973067.2904189/150‑1 (MQ=60)
                                                                                                                                                GTATT‑AAATAAAAATATTCCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAACCACCTCGTATTAATGGACGAGTGCCGGTCCTGTCGGCACAGGAAGCGG       <  SRR19973067.1192896/150‑1 (MQ=60)
                                                                                                                                                   TT‑AAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAACCACCTCGTATTAATGGACGAGTGCCGGTCCTGTCGGCACAGGAAGCGGTGA    >  SRR19973067.1159109/1‑150 (MQ=60)
                                                                                                                                                   TT‑AAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAACCACCTCGTATTAATGGACGAGTGCCGGTCCTGTCGGCACAGGAAGCGGTGA    <  SRR19973067.1149174/150‑1 (MQ=60)
                                                                                                                                                   TT‑AAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAACCACCTCGTATTAATGGACGAGTGCCGGTCCTGTCGGCACAGGAAGCGGTGA    >  SRR19973067.2584618/1‑150 (MQ=60)
                                                                                                                                                   TT‑AAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAACCACCTCGTATTAATGGACGAGTGCCGGTCCTGTCGGCACAGGAAGCGGTGA    >  SRR19973067.2976683/1‑150 (MQ=60)
                                                                                                                                                    T‑AAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAACCACCTCGTATTAATGGACGAGTGCCGGTCCTGTCGGCACAGGAAGCGGTGAA   >  SRR19973067.187251/1‑150 (MQ=60)
                                                                                                                                                      AAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAACCACCTCGTATTAATGGACGAGTGCCGGTCCTGTCGGCACAGGAAGCGGTGAAT  >  SRR19973067.1425971/1‑150 (MQ=60)
                                                                                                                                                      AAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAACCACCTCGTATTAATGGACGAGTGCCGGTCCTGTCGGCACAGGAAGCGGTGAAT  >  SRR19973067.2410596/1‑150 (MQ=60)
                                                                                                                                                      AAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAACCACCTCGTATTAATGGACGAGTGCCGGTCCTGTCGGCACAGGAAGCGGTGAAT  >  SRR19973067.2410599/1‑150 (MQ=60)
                                                                                                                                                      AAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAACCACCTCGTATTAATGGACGAGTGCCGGTCCTGTCGGCACAGGAAGCGGTGAAT  >  SRR19973067.2536603/1‑150 (MQ=60)
                                                                                                                                                      |                                                                                                                                                     
TTTTGGTGCGGTAAAAAAAGCGTCTGCGCCAGGGCAAATCTCGGTAAATGATTTGCGCACGGTATTAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATT‑TAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAACCACCTCGTATTAATGGACGAGTGCCGGTCCTGTCGGCACAGGAAGCGGTGAAT  >  CP009273/1769614‑1769912

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑