Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F368 I0 R1 1472 214.4 7116750 95.9% 6824963 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,077,212 A→T 90.6% N326Y (AAT→TAT)  sbcB → exodeoxyribonuclease I; exonuclease I

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,077,2120AT90.6% 669.5 / 49.6 223N326Y (AAT→TAT) sbcBexodeoxyribonuclease I; exonuclease I
Reads supporting (aligned to +/- strand):  ref base A (9/12);  new base T (96/106);  total (105/118)
Fisher's exact test for biased strand distribution p-value = 8.19e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.93e-01

Only 100 of 225 total aligned reads displayed.

GACACATTGCGCGAGCGTTTATATACCGCAAAAACCGATCTTGGCGATAACGCCGCCGTTCCGGTTAAGCTGGTGCATATCAATAAATGTCCGGTGCTGGCCCAGGCGAATACGCTACGCCCGGAAGATGCCGACCGACTGGGAATTAATCGTCAGCATTGCCTCGATAACCTGAAAATTCTGCGTGAAAATCCGCAAGTGCGCGAAAAAGTGGTGGCGATATTCGCGGAAGCCGAACCGTTTACGCCTTCAGATAACGTGGATGCACAGCTTTATAACGGCTTTTTCAGTG  >  CP009273/2077065‑2077356
                                                                                                                                                   |                                                                                                                                                
gACACATTGCGCGAGCGTTTATATACCGCAAAAACCGATCTTGGCGATAACGCCGCCGTTCCGGTTAAGCTGGTGCATATCAATAAATGTCCGGTGCTGGCCCAGGCGAATACGCTACGCCCGGAAGATGCCGACCGACTGGGAATTa                                                                                                                                                  <  1:2737836/148‑1 (MQ=255)
  cacaTTGCGCGAGCGTTTATATACCGCAAAAACCGATCTTGGCGATAACGCCGCCGTTCCGGTTAAGCTGGTGCATATCAATAAATGTCCGGTGCTGGCCCAGGCGAATACGCTACGCCCGGAAGATGCCGACCGACTGGGAATTTAt                                                                                                                                                >  2:667072/1‑148 (MQ=255)
   acaTTGCGCGAGCGTTTATATACCGCAAAAACCGATCTTGGCGATAACGCCGCCGTTCCGGTTAAGCTGGTGCATATCAATAAATGTCCGGTGCTGGCCCAGGCGAATACGCTACGCCCGGAAGATGCCGACCGACTGGGAATTTATc                                                                                                                                               >  1:254527/1‑148 (MQ=255)
   acaTTGCGCGAGCGTTTATATACCGCAAAAACCGATCTTGGCGATAACGCCGCCGTTCCGGTTAAGCTGGTGCATATCAATAAATGTCCGGTGCTGGCCCAGGCGAATACGCTACGCCCGGAAGATGCCGACCGACTGGGAATTAATc                                                                                                                                               >  2:610577/1‑148 (MQ=255)
    caTTGCGCGAGCGTTTATATACCGCAAAAACCGATCTTGGCGATAACGCCGCCGTTCCGGTTAAGCTGGTGCATATCAATAAATGTCCGGTGCTGGCCCAGGCGAATACGCTACGCCCGGAAGATGCCGACCGACTGGGAATTTATCg                                                                                                                                              >  2:1319415/1‑148 (MQ=255)
     aTTGCGCGAGCGTTTATATACCGCAAAAACCGATCTTGGCGATAACGCCGCCGTTCCGGTTAAGCTGGTGCATATCAATAAATGTCCGGTGCTGGCCCAGGCGAATACGCTACGCCCGGAAGATGCCGACCGACTGGGAATTTATCGt                                                                                                                                             <  2:1256583/148‑1 (MQ=255)
     aTTGCGCGAGCGTTTATATACCGCAAAAACCGATCTTGGCGATAACGCCGCCGTTCCGGTTAAGCTGGTGCATATCAATAAATGTCCGGTGCTGACCCAGGCGAATACGCTACGCCCGGAAGATGCCGACCGACTGGGAATTTATCGt                                                                                                                                             >  2:1108436/1‑148 (MQ=255)
      ttGCGCGAGCGTTTATATACCGCAAAAACCGATCTTGGCGATAACGCCGCCGTTCCGGTTAAGCTGGTGCATATCAATAAATGTCCGGTGCTGGCCCAGGCGAATACGCTACGCCCGGAAGATGCCGACCGACTGGGAATTTATCGTc                                                                                                                                            <  1:307914/148‑1 (MQ=255)
       tGCGCGAGCGTTTATATACCGCAAAAACCGATCTTGGCGATAACGCCGCCGTTCCGGTTAAGCTGGTGCATATCAATAAATGTCCGGTGCTGGCCCAGGCGAATACGCTACGCCCGGAAGATGCCGACCGACTGGGAATTTATCGTCa                                                                                                                                           >  2:1079298/1‑148 (MQ=255)
          gcgAGCGTTTATATACCGCAAAAACCGATCTTGGCGATAACGCCGCCGTTCCGGTTAAGCTGGTGCATATCAATAAATGTCCGGTGCTGGCCCAGGCGAATACGCTACGCCCGGAAGATGCCGACCGACTGGGAATTTATCGTCAGCa                                                                                                                                        <  2:2932726/148‑1 (MQ=255)
           cgAGCGTTTATATACCGCAAAAACCGATCTTGGCGATAACGCCGCCGTTCCGGTTAAGCTGGTGCATATCAATAAATGTCCGGTGCTGGCCCAGGCGAATACGCTACGCCCGGAAGATGCCGACCGACTGGGAATTTATCGTCAGCAt                                                                                                                                       <  1:1325045/148‑1 (MQ=255)
           cgAGCGTTTATATACCGCAAAAACCGATCTTGGCGATAACGCCGCCGTTCCGGTTAAGCTGGTGCATATCAATAAATGTCCGGTGCTGGCCCAGGCGAATACGCTACGCCCGGAAGATGCCGACCGACTGGGAATTTATCGTCAGCAt                                                                                                                                       <  1:1814268/148‑1 (MQ=255)
            gAGCGTTTATATACCGCAAAAACCGATCTTGGCGATAACGCCGCCGTTCCGGTTAAGCTGGTGCATATCAATAAATGTCCGGTGCTGGCCCAGGCGAATACGCTACGCCCGGAAGATGCCGACCGACTGGGAATTTATCGTCAGCAtt                                                                                                                                      <  2:1803305/148‑1 (MQ=255)
             aGCGTTTATATACCGCAAAAACCGATCTTGGCGATAACGCCGCCGTTCCGGTTAAGCTGGTGCATATCAATAAATGTCCGGTGCTGGCCCAGGCGAATACGCTACGCCCGGAAGATGCCGACCGACTGGGAATTTATCGTCAGCATTg                                                                                                                                     >  1:1242792/1‑148 (MQ=255)
              gCGTTTATATACCGCAAAAACCGATCTTGGCGATAACGCCGCCGTTCCGGCTAAGCTGGTGCATATCAATAAATGTCCGGTGCTGGCCCAGGCGAATACGCTACGCCCGGAAGATGCCGACCGACTGGGAATTTATCGTCAGCATTGc                                                                                                                                    <  2:2522081/148‑1 (MQ=255)
               cGTTTATATACCGCAAAAACCGATCTTGGCGATAACGCCGCCGTTCCGGTTAAGCTGGTGCATATCAATAAATGTCCGGTGCTGGCCCAGGCGAATACGCTACGCCCGGAAGATGCCGACCGACTGGGAATTTATCGTCAGCATTGcc                                                                                                                                   <  2:18374/148‑1 (MQ=255)
               cGTTTATATACCGCAAAAACCGATCTTGGCGATAACGCCGCCGTTCCGGTTAAGCTGGTGCATATCAATAAATGTCCGGTGCTGGCCCAGGCGAATACGCTACGCCCGGAAGATGCCGACCGACTGGGAATTTATCGTCAGCATTGcc                                                                                                                                   >  2:917684/1‑148 (MQ=255)
                 tttATATACCGCAAAAACCGATCTTGGCGATAACGCCGCCGTTCCGGTTAAGCTGGTGCATATCAATAAATGTCCGGTGCTGGCCCAGGCGAATACGCTACGCCCGGAAGATGCCGACCGACTGGGAATTTATCGTCAGCATTGCCTc                                                                                                                                 >  2:1477756/1‑148 (MQ=255)
                      ataCCGCAAAAACCGATCTTGGCGATAACGCCGCCGTTCCGGTTAAGCTGGTGCATATCAATAAATGTCCGGTGCTGGCCCAGGCGAATACGCTACGCCCGGATGATGCCGACCGACTGGGAATTTATCGTCAGCATTGCCTCGATaa                                                                                                                            <  1:619903/148‑1 (MQ=255)
                       taCCGCAAAAACCGATCTTGGCGATAACGCCGCCGTTCCGGTTAAGCTGGTGCATATCAATAAATGTCCGGTGCTGGCCCAGGCGAATACGCTACGCCCGGAAGATGCCGACCGACTGGGAATTAATCGTCAGCATTGCCTCGATAAc                                                                                                                           <  2:1354473/148‑1 (MQ=255)
                       taCCGCAAAAACCGATCTTGGCGATAACGCCGCCGTTCCGGTTAAGCTGGTGCATATCAATAAATGTCCGGTGCTGGCCCAGGCGAATACGCTACGCCCGGAAGATGCCGACCGACTGGGAATTAATCGTCAGCATTGCCTCGATAAc                                                                                                                           <  2:3125591/148‑1 (MQ=255)
                       taCCGCAAAAACCGATCTTGGCGATAACGCCGCCGTTCCGGTTAAGCTGGTGCATATCAATAAATGTCCGGTGCTGGCCCAGGCGAATACGCTACGCCCGGAAGATGCCGACCGACTGGGAATTAATCGTCAGCATTGCCTCGATAAc                                                                                                                           <  2:1354453/148‑1 (MQ=255)
                         ccGCAAAAACCGATCTTGGCGATAACGCCGCCGTTCCGGTTAAGCTGGTGCATATCAATAAATGTCCGGTGCTGGCCCAGGCGAATACGCTACGCCCGGAAGATGCCGACCGACTGGGAATTAATCGTCAGCATTGCCTCGATAACCt                                                                                                                         >  1:2317778/1‑148 (MQ=255)
                           gCAAAAACCGATCTTGGCGATAACGCCGCCGTTCCGGTTAAGCTGGTGCATATCAATAAATGTCCGGTGCTGGCCCAGGCGAATACGCTACGCCCGGAAGATGCCGACCGACTGGGAATTTATCGTCAGCATTGCCTCGATAACCTGa                                                                                                                       >  1:1065504/1‑148 (MQ=255)
                               aaaCCGATCTTGGCGATAACGCCGCCGTTCCGGTTAAGCTGGTGCATATCAATAAATGTCCGGTGCTGGCCCAGGCGAATACGCTACGCCCGGAAGATGCCGACCGACTGGGAATTAATCGTCAGCATTGCCTCGATAACCTGAAAAt                                                                                                                   >  2:2270890/1‑148 (MQ=255)
                               aaaCCGATCTTGGCGATAACGCCGCCGTTCCGGTTAAGCTGGTGCATATCAATAAATGTCCGGTGCTGGCCCAGGCGAATACGCTACGCCCGGAAGATGCCGACCGACTGGGAATTAATCGTCAGCATTGCCTCGATAACCTGAAAAt                                                                                                                   <  2:2060815/148‑1 (MQ=255)
                                aaCCGATCTTGGCGATAACGCCGCCGTTCCGGTTAAGCTGGTGCATATCAATAAATGTCCGGTGCTGGCCCAGGCGAATACGCTACGCCCGGAAGATGCCGACCGACTGGGAATTTATCGTCAGCATTGCCTCGATAACCTGAAAAtt                                                                                                                  <  1:768620/148‑1 (MQ=255)
                                 aCCGATCTTGGCGATAACGCCGCCGTTCCGGTTAAGCTGGTGCATATCAATAAATGTCCGGTGCTGGCCCAGGCGAATACGCTACGCCCGGAAGATGCCGACCGACTGGGAATTTATCGTCAGCATTGCCTCGATAACCTGAAAATTc                                                                                                                 >  2:2393605/1‑148 (MQ=255)
                                 aCCGATCTTGGCGATAACGCCGCCGTTCCGGTTAAGCTGGTGCATATCAATAAATGTCCGGTGCTGGCCCAGGCGAATACGCTACGCCCGGAAGATGCCGACCGACTGGGAATTTATCGTCAGCATTGCCTCGATAACCTGAAAATTc                                                                                                                 >  2:887829/1‑148 (MQ=255)
                                  ccGATCTTGGCGATAACGCCGCCGTTCCGGTTTAGCTGGTGCATATCAATAAATGTCCGGTGCTGGCCCAGGCGAATACGCTACGCCCGGAAGATTCCGACCGACTGGGAATTTATCGTCAGCATTGCCTCGATAACCTGAAAATTCt                                                                                                                <  2:445313/148‑1 (MQ=255)
                                  ccGATCTTGGCGATAACGCCGCCGTTCCGGTTAAGCTGGTGCATATCAATAAATGTCCGGTGCTGGCCCAGGCGAATACGCTACGCCCGGAAGATGCCGACCGACTGGGAATTTATCGTCAGCATTGCCTCGATAACCTGAAAATTCt                                                                                                                >  1:2398061/1‑148 (MQ=255)
                                   cGATCTTGGCGATAACGCCGCCGTTCCGGTTAAGCTGGTGCATATCAATAAATGTCCGGTGCTGGCCCAGGCGAATACGCTACGCCCGGAAGATGCCGACCGACTGGGAATTTATCGTCAGCATTGCCTCGATAACCTGAAAATTCTg                                                                                                               <  1:2950265/148‑1 (MQ=255)
                                     aTCTTGGCGATAACGCCGCCGTTCCGGGTAAGCTGGTGCATATCAATAAATGTCCGGTGCTGGCCCAGGCGAATACGCTACGCCCGGAAGATGCCGACCGACTGGGAATTTATAGTCAGCAATGCCTCGATAACCTTAAAAttattag                                                                                                             >  2:3278206/1‑143 (MQ=255)
                                       cTTGGCGATAACGCCGCCGTTCCGGTTAAGCTGGTGCATATCAATAAATGTCCGGTGCTGGCCCAGGCGAATACGCTACGCCCGGAAGATGCCGACCGACTGGGAATTTATCGTCAGCATTGCCTCGATAACCTGAAAATTCTGCGTg                                                                                                           >  2:2044225/1‑148 (MQ=255)
                                             gATAACGCCGCCGTTCCGGTTAAGCTGGTGCATATCAATAAATGTCCGGTGCTGGCCCAGGCGAATACGCTACGCCCGGAAGATGCCGACCGACTGGGAATTTATCGTCAGCATTGCCTCGATAACCTGAAAATTCTGCGTGAAAATc                                                                                                     <  2:3096706/148‑1 (MQ=255)
                                              aTAACGCCGCCGTTCCGGTTAAGCTGGTGCATATCAATAAATGTCCGGTGCTGGCCCAGGCGAATACGCTACGCCCGGAAGATGCCGACCGACTGGGAATTTATCGTCAGCATTGCCTCGATAACCTGAAAATTCTGCGTGAAAATcc                                                                                                    >  2:1625503/1‑148 (MQ=255)
                                              aTAACGCCGCCGTTCCGGTTAAGCTGGTGCATATCAATAAATGTCCGGTGCTGGCCCAGGCGAATACGCTACGCCCGGAAGATGCCGACCGACTGGGAATTTATCGTCAGCATTGCCTCGATAACCTGAAAATTCTGCGTGAAAATcc                                                                                                    >  2:2828472/1‑148 (MQ=255)
                                               tAACGCCGCCGTTCCGGTTAAGCTGGTGCATATCAATAAATGTCCGGTGCTGGCCCAGGCGAATACGCTACGCCCGGAAGATGCCGACCGACTGGGAATTTATCGTCAGCATTGCCTCGATAACCTGAAAATTCTGCGTGAAAATCCg                                                                                                   >  1:686544/1‑148 (MQ=255)
                                               tAACGCCGCCGTTCCGGTTAAGCTGGTGCATATCAATAAATGTCCGGTGCTGGCCCAGGCGAATACGCTACGCCCGGAAGATGCCGACCGACTGGGAATTAATCGTCAGCATTGCCTCGATAACCTGAAAATTCTGCGTGAAAATCCg                                                                                                   >  2:848651/1‑148 (MQ=255)
                                                 aCGCCGCCGTTCCGGTTAAGCTGGTGCATATCAATAAATGTCCGGTGCTGGCCCAGGCGAATACGCTACGCCCGGAAGATGCCGACCGACTGGGAATTTATCGTCAGCATTGCCTCGATAACCTGAAAATTCTGCGTGAAAATCCGCa                                                                                                 >  1:487224/1‑148 (MQ=255)
                                                  cgccgcCGTTCCGGTTAAGCTGGTGCATATCAATAAATGTCCGGTGCTGGCCCAGGCGAATACGCTACGCCCGGAAGATGCCGACCGACTGGGAATTTATCGTCAGCATTGCCTCGATAACCTGAAAATTCTGCGTGAAAATCCGCaa                                                                                                <  2:2001073/148‑1 (MQ=255)
                                                  cgccgcCGTTCCGGTTAAGCTGGTGCATATCAATAAATGTCCGGTGCTGGCCCAGGCGAATACGCTACGCCCGGAAGATGCCGACCGACTGGGAATTTATCGTCAGCATTGCCTCGATAACCTGAAAATTCTGCGTGAAAATCCGCaa                                                                                                <  2:203877/148‑1 (MQ=255)
                                                   gccgccGTTCCGGTTAAGCTGGTGCATATCAATAAATGTCCGGTGCTGGCCCAGGCGAATACGCTACGCCCGGAAGATGCCGACCGACTGGGAATTTATCGTCAGCATTGCCTCGATAACCTGAAAATTCTGCGTGAAAATCCGCAAg                                                                                               <  1:1926419/148‑1 (MQ=255)
                                                   gccgccGTTCCGGTTAAGCTGGTGCATATCAATAAATGTCCGGTGCTGGCCCAGGCGAATACGCTACGCCCGGAAGATGCCGACCGACTGGGAATTTATCGTCAGCATTGCCTCGATAACCTGAAAATTCTGCGTGAAAATCCGCAAg                                                                                               <  1:1319415/148‑1 (MQ=255)
                                                   gccgccGTTCCGGTTAAGCTGGTGCATATCAATAAATGTCCGGTGCTGGCCCAGGCGAATACGCTACGCCCGGAAGATGCCGACCGACTGGGAATTTATCGTCAGCATTGCCTCGATAACCTGAAAATTCTGCGTGAAAATCCGCAAg                                                                                               <  1:1926381/148‑1 (MQ=255)
                                                     cgccgTTCCGGTTAAGCTGGTGCATATCAATAAATGTCCGGTGCTGGCCCAGGCGAATACGCTACGCCCGGAAGATGCCGACCGACTGGGAATTTATCGTCAGCATTGCCTCGATAACCTGAAAATTCTGCGTGAAAATCCGCAAGTg                                                                                             <  2:2107556/148‑1 (MQ=255)
                                                       ccgTTCCGGTTAAGCTGGTGCATATCAATAAATGTCCGGTGCTGGCCCAGGCGAATACGCTACGCCCGGAAGATGCCGACCGACTGGGAATTTATCGTCAGCATTGCCTCGATAACCTGAAAATTCTGCGTGAAAATCCGCAAGTgcg                                                                                           >  1:3090509/1‑148 (MQ=255)
                                                               gTTAAGCTGGTGCATATCAATAAATGTCCGGTGCTGGCCCAGGCGAATACGCTACGCCCGGAAGATGCCGACCGACTGGGAATTTATCGTCAGCATTGCCTCGATAACCTGAAAATTCTGCGTGAAAATCCGCAAGTGCGCGAAAAAg                                                                                   <  2:342715/148‑1 (MQ=255)
                                                                 tAAGCTGGTGCATATCAATAAATGTCCGGTGCTGGCCCAGGCGAATACGCTACGCCCGGAAGATGCCGACCGACTGGGAATTTATCGTCAGCATTGCCTCGATAACCTGAAAATTCTGCGTGAAAATCCGCAAGTGCGCGAAAAAgtg                                                                                 >  1:80658/1‑148 (MQ=255)
                                                                 tAAGCTGGTGCATATCAATAAATGTCCGGTGCTGGCCCAGGCGAATACGCTACGCCCGGAAGATGCCGACCGACTGGGAATTTATCGTCAGCATTGCCTCGATAACCTGAAAATTCTGCGTGAAAATCCGCAAGTGCGCGAAAAAgtg                                                                                 >  2:2173552/1‑148 (MQ=255)
                                                                  aaGCTGGTGCATATCAATAAATGTCCGGTGCTGGCCCAGGCGAATACGCTACGCCCGGAAGATGCCGACCGACTGGGAATTTATCGTCAGCATTGCCTCGATAACCTGAAAATTCTGCGTGAAAATCCGCAAGTGCGCGAAAAAgtgg                                                                                <  2:2646716/148‑1 (MQ=255)
                                                                       ggTGCATATCAATAAATGTCCGGTGCTGGCCCAGGCGAATACGCTACGCCCGGAAGATGCCGACCGACTGGGAATTTATCGTCAGCATTGCCTCGATAACCTGAAAATTCTGCGTGAAAATCCGCAAGTGCGCGAAAAAGTGGTGGCg                                                                           <  2:640/148‑1 (MQ=255)
                                                                       ggTGCATATCAATAAATGTCCGGTGCTGGCCCAGGCGAATACGCTACGCCCGGAAGATGCCGACCGACTGGGAATTTATCGTCAGCATTGCCTCGATAACCTGAAAATTCTGCGTGAAAATCCGCAAGTGCGCGAAAAAGTGGTGGCg                                                                           <  2:3265918/148‑1 (MQ=255)
                                                                       ggTGCATATCAATAAATGTCCGGTGCTGGCCCAGGCGAATACGCTACGCCCGGAAGATGCCGACCGACTGGGAATTTATCGTCAGCATTGCCTCGATAACCTGAAAATTCTGCGTGAAAATCCGCAAGTGCGCGAAAAAGTGGTGGCg                                                                           <  2:679/148‑1 (MQ=255)
                                                                       ggTGCATATCAATAAATGTCCGGTGCTGGCCCAGGCGAATACGCTACGCCCGGAAGATGCCGACCGACTGGGAATTTATCGTCAGCATTGCCTCGATAACCTGAAAATTCTGCGTGAAAATCCGCAAGTGCGCGAAAAAGTGGTGGCg                                                                           >  2:2480044/1‑148 (MQ=255)
                                                                        gTGCATATCAATAAAGGTCCGGTGCTGGCCCAGGCGAATACGCTACGCCCGGAAGATGCCGACCGACTGGGAATTTATCGTCAGCATTGCCTCGATAACCTGAAAATTCTGCGTGAAAATCCGCAAGTGCGCGAAAAAGTGGTGGCGa                                                                          <  1:3416698/148‑1 (MQ=255)
                                                                           cATATCAATAAATGTCCGGTGCTGGCCCAGGCGAATACGCTACGCCCGGAAGATGCCGACCGACTGGGAATTTATCGTCAGCATTGCCTCGATAACCTGAAAATTCTGCGTGAAAATCCGCAAGTGCGCGAAAAAGTGGTGGCGatat                                                                       <  1:1734846/148‑1 (MQ=255)
                                                                            atatCAATAAATGTCCGGTGCTGGCCCAGGCGAATACGCTACGCCCGGAAGATGCCGACCGACTGGGAATTTATCGTCAGCATTGCCTCGATAACCTGAAAATTCTGCGTGAAAATCCGCAAGTGCGCGAAAAAGTGGTGGCGATAtt                                                                      >  1:2139794/1‑148 (MQ=255)
                                                                              atCAATAAATGTCCGGTGCTGGCCCAGGCGAATACGCTACGCCCGGAAGATGCCGACCGACTGGGAATTTATCGTCAGCATTGCCTCGATAACCTGAAAATTCTGCGTGAAAATCCGCAAGTGCGCGAAAAAGTGGTGGCGATATTcg                                                                    >  2:2418376/1‑148 (MQ=255)
                                                                                  aTAAATGTCCGGTGCTGGCCCAGGCGAATACGCTACGCCCGGAAGATGCCGACCGACTGGGAATTTATCGTCAGCATTGCCTCGATAACCTGAAAATTCTGCGTGAAAATCCGCAAGTGCGCGAAAAAGTGGTGGCGATATTCGCGGa                                                                <  2:2648409/148‑1 (MQ=255)
                                                                                  aTAAATGTCCGGTGCTGGCCCAGGCGAATACGCTACGCCCGGAAGATGCCGACCGACTGGGAATTTATCGTCAGCATTGCCTCGATAACCTGAAAATTCTGCGTGAAAATCCGCAAGTGCGCGAAAAAGTGGTGGCGATATTCGCGGa                                                                <  1:2047240/148‑1 (MQ=255)
                                                                                    aaaTGTCCGGTGCTGGCCCAGGCGAATACGCTACGCCCGGAAGATGCCGACCGACTGGGAATTTATCGTCAGCATTGCCTCGATAACCTGAAAATTCTGCGTGAAAATCCGCAAGTGCGCGAAAAAGTGGTGGCGATATTCGCGGAAg                                                              <  1:2746293/148‑1 (MQ=255)
                                                                                    aaaTGTCCGGTGCTGGCCCAGGCGAATACGCTACGCCCGGAAGATGCCGACCGACTGGGAATTTATCGTCAGCATTGCCTCGATAACCTGAAAATTCTGCGTGAAAATCCGCAAGTGCGCGAAAAAGTGGTGGCGATATTCGCGGAAg                                                              <  2:228965/148‑1 (MQ=255)
                                                                                     aaTGTCCGGTGCTGGCCCAGGCGAATACGCTACGCCCGGAAGATGCCGACCGACTGGGAATTAATCGTCAGCATTGCCTCGATAACCTGAAAATTCTGCGTGAAAATCCGCAAGTGCGCGAAAAAGTGGTGGCGATATTCGCGGAAGc                                                             >  2:3088049/1‑148 (MQ=255)
                                                                                      aTGTCCGGTGCTGGCCCAGGCGAATACGCTACGCCCGGAAGATGCCGACCGACTGGGAATTTATCGTCAGCATTGCCTCGATAACCTGAAAATTCTGCGTGAAAATCCGCAAGTGCGCGAAAAAGTGGTGGCGATATTCGCGGAAGcc                                                            >  1:316522/1‑148 (MQ=255)
                                                                                             gTGCTGGCCCAGGCGAATACGCTACGCCCGGAAGATGCCGACCGACTGGGAATTTATCGTCAGCATTGCCTCGATAACCTGAAAATTCTGCGTGAAAATCCGCAAGTGCGCGAAAAAGTGGTGGCGATATTCGCGGAAGCCGAACCGt                                                     <  2:3204913/148‑1 (MQ=255)
                                                                                              tGCTGGCCCAGGCGAATACGCTACGCCCGGAAGATGCCGACCGACTGGGAATTTATCGTCAGCATTGCCTCGATAACCTGAAAATTCTGCGTGAAAATCCGCAAGTGCGCGAAAAAGTGGTGGCGATATTCGCGGAAGCCGAACCGtt                                                    <  1:1367764/148‑1 (MQ=255)
                                                                                              tGCTGGCCCAGGCGAATACGCTACGCCCGGAAGATGCCGACCGACTGGGAATTTATCGTCAGCATTGCCTCGATAACCTGAAAATTCTGCGTGAAAATCCGCAAGTGCGCGAAAAAGTGGTGGCGATATTCGCGGAAGCCGAACCGtt                                                    <  1:981801/148‑1 (MQ=255)
                                                                                               gCTGGCCCAGGCGAATACGCTACGCCCGGAAGATGCCGACCGACTGGGAATTTATCGTCAGCATTGCCTCGATAACCTGAAAATTCTGCGTGAAAATCCGCAAGTGCGCGAAAAAGTGGTGGCGATATTCGCGGAAGCCGAACCGttt                                                   >  2:2452654/1‑148 (MQ=255)
                                                                                               gCTGGCCCAGGCGAATACGCTACGCCCGGAAGATGCCGACCGACTGGGAATTTATCGTCAGCATTGCCTCGATAACCTGAAAATTCTGCGTGAAAATCCGCAAGTGCGCGAAAAAGTGGTGGCGATATTCGCGGAAGCCGAACCGttt                                                   <  1:1597139/148‑1 (MQ=255)
                                                                                               gCTGGCCCAGGCGAATACGCTACGCCCGGAAGATGCCGACCGACTGGGAATTAATCGTCAGCATTGCCTCGATAACCTGAAAATTCTGCGTGAAAATCCGCAAGTGCGCGAAAAAGTGGTGGCGATATTCGCGGAAGCCGAACCGttt                                                   <  1:2270890/148‑1 (MQ=255)
                                                                                                cTGGCCCAGGCGAATACGCTACGCCCGGAAGATGCCGACCGACTGGGAATTTATCGTCAGCATTGCCTCGATAACCTGAAAATTCTGCGTGAAAATCCGCAAGTGCGCGAAAAAGTGGTGGCGATATTCGCGGAAGCCGAACCGTTTa                                                  >  1:1710804/1‑148 (MQ=255)
                                                                                                cTGGCCCAGGCGAATACGCTACGCCCGGAAGATGCCGACCGACTGGGAATTTATCGTCAGCATTGCCTCGATAACCTGAAAATTCTGCGTGAAAATCCGCAAGTGCGCGAAAAAGTGGTGGCGATATTCGCGGAAGCCGAACCGTTTa                                                  >  1:2365744/1‑148 (MQ=255)
                                                                                                   gCCCAGGCGAATACGCTACGCCCGGAAGATGCCGACCGACTGGGAATTTATCGTCAGCATTGCCTCGATAACCTGAAAATTCTGCGTGAAAATCCGCAAGTGCGCGAAAAAGTGGTGGCGATATTCGCGGAAGCCGAACCGTTTACGc                                               >  1:314551/1‑148 (MQ=255)
                                                                                                   gCCCAGGCGAATACGCTACGCCCGGAAGATGCCGACCGACTGGGAATTTATCGTCAGCATTGCCTCGATAACCTGAAAATTCTGCGTGAAAATCCGCAAGTGCGCGAAAAAGTGGTGGCGATATTCGCGGAAGCCGAACCGTTTACGc                                               >  2:1882269/1‑148 (MQ=255)
                                                                                                   gCCCAGGCGAATACGCTACGCCCGGAAGATGCCGACCGACTGGGAATTTATCGTCAGCATTGCCTCGATAACCTGAAAATTCTGCGTGAAAATCCGCAAGTGCGCGAAAAAGTGGTGGCGATATTCGCGGAAGCCGAACCGTTTACGc                                               >  2:2781743/1‑148 (MQ=255)
                                                                                                   gCCCAGGCGAATACGCTACGCCCGGAAGATGCCGACCGACTGGGAATTTATCGTCAGCATTGCCTCGATAACCTGAAAATTCTGCGTGAAAATCCGCAAGTGCGCGAAAAAGTGGTGGCGATATTCGCGGAAGCCGAACCGTTTACGc                                               >  1:61434/1‑148 (MQ=255)
                                                                                                    cccAGGCGAATACGCTACGCCCGGAAGATGCCGACCGACTGGGAATTTATCGTCAGCATTGCCTCGATAACCTGAAAATTCTGCGTGAAAATCCGCAAGTGCGCGAAAAAGTGGTGGCGATATTCGCGGAAGCCGAACCGTTTACGcc                                              <  1:1611404/148‑1 (MQ=255)
                                                                                                    cccAGGCGAATACGCTACGCCCGGAAGATGCCGACCGACTGGGAATTTATCGTCAGCATTGCCTCGATAACCTGAAAATTCTGCGTGAAAATCCGCAAGTGCGCGAAAAAGTGGTGGCGATATTCGCGGAAGCCGAACCGTTTACGcc                                              <  2:1699647/148‑1 (MQ=255)
                                                                                                    cccAGGCGAATACGCTACGCCCGGAAGATGCCGACCGACTGGGAATTTATCGTCAGCATTGCCTCGATAACCTGAAAATTCTGCGTGAAAATCCGCAAGTGCGCGAAAAAGTGGTGGCGATATTCGCGGAAGCCGAACCGTTTACGcc                                              <  1:673633/148‑1 (MQ=255)
                                                                                                       aGGCGAATACGCTACGCCCGGAAGATGCCGACCGACTGGGAATTTATCGTCAGCATTGCCTCGATAACCTGAAAATTCTGCGTGAAAATCCGCAAGTGCGCGAAAAAGTGGTGGCGATATTCGCGGAAGCCGAACCGTTTACGCCTTc                                           <  2:1921792/148‑1 (MQ=255)
                                                                                                       aGGCGAATACGCTACGCCCGGAAGATGCCGACCGACTGGGAATTTATCGTCAGCATTGCCTCGATAACCTGAAAATTCTGCGTGAAAATCCGCAAGTGCGCGAAAAAGTGGTGGCGATATTCGCGGAAGCCGAACCGTTTACGCCTTc                                           <  2:2608426/148‑1 (MQ=255)
                                                                                                                  ctacgcCCGGAAGATGCCGACCGACTGGGAATTTATCGTCAGCATTGCCTCGATAACCTGAAAATTCTGCGTGAAAATCCGCAAGTGCGCGAAAAAGTGGTGGCGATATTCGCGGAAGCCGAACCGTTTACGCCTTCAGATAACGTgg                                <  1:1625503/148‑1 (MQ=255)
                                                                                                                     cgcCCGGAAGATGCCGACCGACTGGGAATTTATCGTCAGCATTGCCTCGATAACCTGAAAATTCTGCGTGAAAATCCGCAAGTGCGCGAAAAAGTGGTGGCGATATTCGCGGAAGCCGAACCGTTTACGCCTTCAGATAACGTGGATg                             <  1:1129876/148‑1 (MQ=255)
                                                                                                                        ccGGAAGATGCCGACCGACTGGGAATTTATCGTCAGCATTGCCTCGATAACCTGAAAATTCTGCGTGAAAATCCGCAAGTGCGCGAAAAAGTGGTGGCGATATTCGCGGAAGCCGAACCGTTTACGCCTTCAGATAACGTGGATGcac                          <  2:1394007/148‑1 (MQ=255)
                                                                                                                        ccGGAAGATGCCGACCGACTGGGAATTTATCGTCAGCATTGCCTCGATAACCTGAAAATTCTGCGTGAAAATCCGCAAGTGCGCGAAAAAGTGGTGGCGATATTCGCGGAAGCCGAACCGTTTACGCCTTCAGATAACGTGGATGcac                          <  1:1435504/148‑1 (MQ=255)
                                                                                                                           gAAGATGCCGACCGACTGGGAATTTATCGTCAGCATTGCCTCGATAACCTGAAAATTCTGCGTGAAAATCCGCAAGTGCGCGAAAAAGTGGTGGCGATATTCGCGGAAGCCGAACCGTTTACGCCTTCAGATAACGTGGATGCACAGc                       >  1:1840286/1‑148 (MQ=255)
                                                                                                                            aaGATGCCGACCGACTTGGAATTTATCGTCAGCATTGCCTAGATAACCTGAAAATTCTGCGTGAAAATCCGCAAGTGCGCGAAAAAGTGGTGGCGATATTAGCTGAAGCCGAACCGTTTACGCCTTCAGATAACGTGGATGCACAGCt                      <  2:3005910/148‑1 (MQ=255)
                                                                                                                            aaGATGCCGACCGACTGGGAATTTATCGTCAGCATTGCCTCGATAACCTGAAAATTCTGCGTGAAAATCCGCAAGTGCGCGAAAAAGTGGTGGCGATATTCGCGGAAGCCGAACCGTTTACGCCTTCAGATAACGTGGATGCACAGCt                      >  1:3499843/1‑148 (MQ=255)
                                                                                                                              gATGCCGACCGACTGGGAATTTATCGTCAGCATTGCCTCGATAACCTGAAAATTCTGCGTGAAAATCCGCAAGTGCGCGAAAAAGTGGTGGCGATATTCGCGGAAGCCGAACCGTTTACGCCTTCAGATAACGTGGATGCACAGCttt                    <  1:3539616/148‑1 (MQ=255)
                                                                                                                               aTGCCGATCGACTGGGAATTTATCGTCAGCATTGCCTCGATAACCTGAAAATTCTGCGTGAAAATCCGCAAGTGCGCGAAAAAGTGGTGGCGATATTCGCGGAAGCCGAACCGTTTACGCCTTCAGATAACGTGGATGCACAGCTTta                   <  2:720836/148‑1 (MQ=255)
                                                                                                                               aTGCCGACCGACTGGGAATTTATCGTCAGCATTGCCTCGATAACCTGAAAATTCTGCGTGAAAATCCGCAAGTGCGCGAAAAAGTGGTGGCGATATTCGCGGAAGCCGAACCGTTTACGCCTTCAGATAACGTGGATGCACAGCTTta                   <  1:3027874/148‑1 (MQ=255)
                                                                                                                                tGCCGACCGACTGGGAATTTATCGTCAGCATTGCCTCGATAACCTGAAAATTCTGCGTGAAAATCCGCAAGTGCGCGAAAAAGTGGTGGCGATATTCGCGGAAGCCGAACCGTTTACGCCTTCAGATAACGTGGATGCACAGCTTtat                  <  1:1086927/148‑1 (MQ=255)
                                                                                                                                  ccgaccgaCTGGGAATTTATCGTCAGCATTGCCTCGATAACCTGAAAATTCTGCGTGAAAATCCGCAAGTGCGCGAAAAAGTGGTGGCGATATTCGCGGAAGCCGAACCGTTTACGCCTTCAGATAACGTGGATGCACAGCTTTATaa                >  2:1228896/1‑148 (MQ=255)
                                                                                                                                     accgacTGGGAATTTATCGTCAGCATTGCCTCGATAACCTGAAAATTCTGCGTGAAAATCCGCAAGTGCGCGAAAAAGTGGTGGCGATATTCGCGGAAGCCGAACCGTTTACGCCTTCAGATAACGTGGATGCACAGCTTTATAACgg             >  1:2005505/1‑148 (MQ=255)
                                                                                                                                      ccgacTGGGAATTTATCGTCAGCAGTGCCTCGATAACCTGAAAATTCTGCGTGAAAATCCGCAAGTGCGCGAAAAAGTGGTGGCGATATTCGCGGAAGCCGAACCGTTTACGCCTTCAGATAACGTGGATGCACAGCTTTATAACGGc            <  2:1438586/148‑1 (MQ=255)
                                                                                                                                          cTGGGAATTTATCGTCAGCATTGCCTCGATAACCTGAAAATTCTGCGTGAAAATCCGCAAGTGCGCGAAAAAGTGGTGGCGATATTCGCGGAAGCCGAACCGTTTACGCCTTCAGATAACGTGGATGCACAGCTTTATAACGGCtttt        <  2:1656958/148‑1 (MQ=255)
                                                                                                                                           tGGGAATTTATCGTCAGCATTGCCTCGATAACCTGAAAATTCTGCGTGAAAATCCGCAAGTGCGCGAAAAAGTGGTGGCGATATTCGCGGAAGCCGAACCGTTTACGCCTTCAGATAACGTGGATGCACAGCTTTATAACGGCttttt       >  1:3341172/1‑148 (MQ=255)
                                                                                                                                            gggAATTTATCGTCAGCATTGCCTCGATAACCTGAAAATTCTGCGTGAAAATCCGCAAGTGCGCGAAAAAGTGGTGGCGATATTCGCGGAAGCCGAACCGTTTACGCCTTCAGATAACGTGGATGCACAGCTTTATAACGGCTTTTTc      <  1:1101348/148‑1 (MQ=255)
                                                                                                                                                aTTTATCGTCAGCATTGCCTCGATAACCTGAAAATTCTGCGTGAAAATCCGCAAGTGCGCGAAAAAGTGGTGGCGATATTCGCGGAAGCCGAACCGTTTACGCCTTCAGATAACGTGGATGCACAGCTTTATAACGGCTTTTTCAGTg  <  1:3470543/148‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                
GACACATTGCGCGAGCGTTTATATACCGCAAAAACCGATCTTGGCGATAACGCCGCCGTTCCGGTTAAGCTGGTGCATATCAATAAATGTCCGGTGCTGGCCCAGGCGAATACGCTACGCCCGGAAGATGCCGACCGACTGGGAATTAATCGTCAGCATTGCCTCGATAACCTGAAAATTCTGCGTGAAAATCCGCAAGTGCGCGAAAAAGTGGTGGCGATATTCGCGGAAGCCGAACCGTTTACGCCTTCAGATAACGTGGATGCACAGCTTTATAACGGCTTTTTCAGTG  >  CP009273/2077065‑2077356

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GACACATTGCGCGAGCGTTTATATACCGCAAAAACCGATCTTGGCGATAACGCCGCCGTTCCGGTTAAGCTGGTGCATATCAATAAATGTCCGGTGCTGGCCCAGGCGAATACGCTACGCCCGGAAGATGCCGACCGACTGGGAATTAATCGTCAGCATTGCCTCGATAACCTGAAAATTCTGCGTGAAAATCCGCAAGTGCGCGAAAAAGTGGTGGCGATATTCGCGGAAGCCGAACCGTTTACGCCTTCAGATAACGTGGATGCACAGCTTTATAACGGCTTTTTCAGTGACGCA  >  CP009273/2077065‑2077361
                                                                                                                                                   |                                                                                                                                                     
GACACATTGCGCGAGCGTTTATATACCGCAAAAACCGATCTTGGCGATAACGCCGCCGTTCCGGTTAAGCTGGTGCATATCAATAAATGTCCGGTGCTGGCCCAGGCGAATACGCTACGCCCGGAAGATGCCGACCGACTGGGAATTTAT                                                                                                                                                     <  SRR19973067.3577816/150‑1 (MQ=60)
GACACATTGCGCGAGCGTTTATATACCGCAAAAACCGATCTTGGCGATAACGCCGCCGTTCCGGTTAAGCTGGTGCATATCAATAAATGTCCGGTGCTGGCCCAGGCGAATACGCTACGCCCGGAAGATGCCGACCGACTGGGAATTTAT                                                                                                                                                     <  SRR19973067.1782507/150‑1 (MQ=60)
GACACATTGCGCGAGCGTTTATATACCGCAAAAACCGATCTTGGCGATAACGCCGCCGTTCCGGTTAAGCTGGTGCATATCAATAAATGTCCGGTGCTGGCCCAGGCGAATACGCTACGCCCGGAAGATGCCGACCGACTGGGAATTAAT                                                                                                                                                     <  SRR19973067.2755534/150‑1 (MQ=60)
GACACATTGCGCGAGCGTTTATATACCGCAAAAACCGATCTTGGCGATAACGCCGCCGTTCCGGTTAAGCTGGTGCATATCAATAAATGTCCGGTGCTGGCCCAGGCGAATACGCTACGCCCGGAAGATGCCGACCGACTGGGAATTAAT                                                                                                                                                     <  SRR19973067.2755520/150‑1 (MQ=60)
 ACACATTGCGCGAGCGTTTATATACCGCAAAAACCGATCTTGGCGATAACGCCGCCGTTCCGGTTAAGCTGGTGCATATCAATAAATGTCCGGTGCTGGCCCAGGCGAATACGCTACGCCCGGAAGATGCCGACCGACTGGGAATTTATC                                                                                                                                                    >  SRR19973067.256268/1‑150 (MQ=60)
     ATTGCGCGAGCGTTTATATACCGCAAAAACCGATCTTGGCGATAACGCCGCCGTTCCGGTTAAGCTGGTGCATATCAATAAATGTCCGGTGCTGGCCCAGGCGAATACGCTACGCCCGGAAGATGCCGACCGACTGGGAATTTATCGTCA                                                                                                                                                <  SRR19973067.1095947/150‑1 (MQ=60)
      TTGCGCGAGCGTTTATATACCGCAAAAACCGATCTTGGCGATAACGCCGCCGTTCCGGTTAAGCTGGTGCATATCAATAAATGTCCGGTGCTGGCCCAGGCGAATACGCTACGCCCGGAAGATGCCGACCGACTGGGAATTTATCGTCAG                                                                                                                                               <  SRR19973067.309918/150‑1 (MQ=60)
           CGAGCGTTTATATACCGCAAAAACCGATCTTGGCGATAACGCCGCCGTTCCGGTTAAGCTGGTGCATATCAATAAATGTCCGGTGCTGGCCCAGGCGAATACGCTACGCCCGGAAGATGCCGACCGACTGGGAATTTATCGTCAGCATTG                                                                                                                                          <  SRR19973067.1334088/150‑1 (MQ=60)
           CGAGCGTTTATATACCGCAAAAACCGATCTTGGCGATAACGCCGCCGTTCCGGTTAAGCTGGTGCATATCAATAAATGTCCGGTGCTGGCCCAGGCGAATACGCTACGCCCGGAAGATGCCGACCGACTGGGAATTTATCGTCAGCATTG                                                                                                                                          <  SRR19973067.1826548/150‑1 (MQ=60)
           CGAGCGTTTATATACCGCAAAAACCGATCTTGGCGATAACGCCGCCGTTCCGGTTAAGCTGGTGCATATCAATAAATGTCCGGTGCTGGCCCAGGCGAATACGCTACGCCCGGAAGATGCCGACCGACTGGGAATTTATCGTCAGCATTG                                                                                                                                          <  SRR19973067.1826981/150‑1 (MQ=60)
           CGAGCGTTTATATACCGCAAAAACCGATCTTGGCGATAACGCCGCCGTTCCGGTTAAGCTGGTGCATATCAATAAATGTCCGGTGCTGGCCCAGGCGAATACGCTACGCCCGGAAGATGCCGACCGACTGGGAATTTATCGTCAGCATTG                                                                                                                                          >  SRR19973067.1297140/1‑150 (MQ=60)
           CGAGCGTTTATATACCGCAAAAACCGATCTTGGCGATAACGCCGCCGTTCCGGTTAAGCTGGTGCATATCAATAAATGTCCGGTGCTGGCCCAGGCGAATACGCTACGCCCGGAAGATGCCGACCGACTGGGAATTTATCGTCAGCATTG                                                                                                                                          >  SRR19973067.1251158/1‑150 (MQ=60)
             AGCGTTTATATACCGCAAAAACCGATCTTGGCGATAACGCCGCCGTTCCGGTTAAGCTGGTGCATATCAATAAATGTCCGGTGCTGGCCCAGGCGAATACGCTACGCCCGGAAGATGCCGACCGACTGGGAATTTATCGTCAGCATTGCC                                                                                                                                        >  SRR19973067.1581447/1‑150 (MQ=60)
                     TAGACCGCAAAAACCGATCTTGGCGATAACGCCGCCGTTCCGGTTAAGCTGGTGCATATCAATAAATGTCCGGTGCTGGCCCAGGCGAATACGCTACGCCCGGAAGATGCCGACCGACTGGGAATTAATCGTCAGCATTGCCTCGATAAC                                                                                                                                <  SRR19973067.3478162/150‑1 (MQ=60)
                      ATACCGCAAAAACCGTTCTTGGCTATAACGCCGCCGTTCCGGTTAAGCTGGTGCATATCAATAAATGTCCGGTGCTGGCCCAGGCGAATACGCTACGCCCGGATGATGCCGACCGACTGGGAATTTATCGTCAGCATTGCCTCGATAACC                                                                                                                               <  SRR19973067.623980/150‑1 (MQ=60)
                      ATACCGCAAAAACCGATCTTGGCGATAACGCCGCCGTTCCGGTTAAGCTGGTGCATATCAATAAATGTCCGGTGCTGGCCCAGGCGAATACGCTACGCCCGGAAGATGCCGACCGACTGGGAATTTATCGTCAGCATTGCCTCGATAACC                                                                                                                               <  SRR19973067.623952/150‑1 (MQ=60)
                       TACCGCAAAAACCGATCTTGGCGATAACGCCGCCGTTCCGGTTAAGCTGGTGCATATCAATAAATGTCCGGTGCTGGCCCAGGCGAATACGCTACGCCCGGAAGATGCCGACCGACTGGGAATTAATCGTCAGCATTGCCTCGATAACCT                                                                                                                              >  SRR19973067.2333022/1‑150 (MQ=60)
                         CCGCAAAAACCGATCTTGGCGATAACGCCGCCGTTCCGGTTAAGCTGGTGCATATCAATAAATGTCCGGTGCTGGCCCAGGCGAATACGCTACGCCCGGAAGATGCCGACCGACTGGGAATTTATCGTCAGCATTGCCTCGATAACCTGA                                                                                                                            >  SRR19973067.1072810/1‑150 (MQ=60)
                            CAAAAACCGATCTTGGCGATAACGCCGCCGTTCCGGTTAAGCTGGTGCATATCAATAAATGTCCGGTGCTGGCCCAGGCGAATACGCTACGCCCGGAAGATGCCGACCGACTGGGAATTTATCGTCAGCATTGCCTCGATAACCTGAAAA                                                                                                                         >  SRR19973067.1403477/1‑150 (MQ=60)
                                AACCGATCTTGGCGATAACGCCGCCGTTCCGGTTAAGCTGGTGCATATCAATAAATGTCCGGTGCTGGCCCAGGCGAATACGCTACGCCCGGAAGATGCCGACCGACTGGGACTTTATCGTCAGCATTGCCTCGATAACCTGAAAATTCT                                                                                                                     >  SRR19973067.2252714/1‑150 (MQ=60)
                                AACCGATCTTGGCGATAACGCCGCCGTTCCGGTTAAGCTGGTGCATATCAATAAATGTCCGGTGCTGGCCCAGGCGAATACGCTACGCCCGGAAGATGCCGACCGACTGGGAATTTATCGTCAGCATTGCCTCGATAACCTGAAAATTCT                                                                                                                     >  SRR19973067.2570563/1‑150 (MQ=60)
                                AACCGATCTTGGCGATAACGCCGCCGTTCCGGTTAAGCTGGTGCATATCAATAAATGTCCGGTGCTGGCCCAGGCGAATACGCTACGCCCGGAAGATGCCGACCGACTGGGAATTTATCGTCAGCATTGCCTCGATAACCTGAAAATTCT                                                                                                                     >  SRR19973067.2413687/1‑150 (MQ=60)
                                AACCGATCTTGGCGATAACGCCGCCGTTCCGGTTAAGCTGGTGCATATCAATAAATGTCCGGTGCTGGCCCAGGCGAATACGCTACGCCCGGAAGATGCCGACCGACTGGGAATTTATCGTCAGCATTGCCTCGATAACCTGAAAATTCT                                                                                                                     <  SRR19973067.773586/150‑1 (MQ=60)
                                AACCGATCTTGGCGATAACGCCGCCGTTCCGGTTAAGCTGGTGCATATCAATAAATGTCCGGTGCTGGCCCAGGCGAATACGCTACGCCCGGAAGATGCCGACCGACTGGGAATTTATCGTCAGCATTGCCTCGATAACCTGAAAATTCT                                                                                                                     >  SRR19973067.1112723/1‑150 (MQ=60)
                                AACCGATCTTGGCGATAACGCCGCCGTTCCGGTTAAGCTGGTGCATATCAATAAATGTCCGGTGCTGGCCCAGGCGAATACGCTACGCCCGGAAGATGCCGACCGACTGGGAATTTATCGTCAGCATTGCCTCGATAACCTGAAAATTCT                                                                                                                     <  SRR19973067.773950/150‑1 (MQ=60)
                                  CCGATCTTGGCGATAACGCCGCCGTTCCGGTTAAGCTGGTGCATATCAATAAATGTCCGGTGCTGGCCCAGGCGAATACGCTACGCCCGGAAGATGCCGACCGACTGGGAATTTATCGTCAGCATTGCCTCGATAACCTGAAAATTCTGC                                                                                                                   >  SRR19973067.2040196/1‑150 (MQ=60)
                                   CGATCTTGGCGATAACGCCGCCGTTCCGGTTAAGCTGGTGCATATCAATAAATGTCCGGTGCTGGCCCAGGCGAATACGCTACGCCCGGAAGATGCCGACCGACTGGGAATTTATCGTCAGCATTGCCTCGATAACCTGAAAATTCTGCG                                                                                                                  <  SRR19973067.2968931/150‑1 (MQ=60)
                                     ATCTTGGCGATAACGCCGCCGTTCCGGTTAAGCTGGTGCATATCAATAAATGTCCGGTGCTGGCCCAGGCGAATACGCTACGCCCGGAAGATGCCGACCGACTGGGAATTTATCGTCAGCATTGCCTCGATAACCTGAAAATTCTGCGTG                                                                                                                >  SRR19973067.3198156/1‑150 (MQ=60)
                                          GGCGATAACGCCGCCGTTCCGGTTAAGCTGGTGCATATCAATAAATGTCCGGTGCTGGCCCAGGCGAATACGCTACGCCCGGAAGATGCCGACCGACTGGGAATTTATCGTCAGCATTGCCTCGATAACCTGAAAATTCTGCGTGAAAAT                                                                                                           <  SRR19973067.1442435/150‑1 (MQ=60)
                                             GATAACGCCGCCGTTCCGGTTAAGCTGGTGCATATCAATAAATGTCCGGTGCTGGCCCAGGCGAATACGCTACGCCCGGAAGATGCCGACCGACTGGGAATTTATCGTCAGCATTGCCTCGATAACCTGAAAATTCTGCGTGAAAATCCG                                                                                                        >  SRR19973067.691204/1‑150 (MQ=60)
                                             GATAACGCCGCCGTTCCGGTTAAGCTGGTGCATATCAATAAATGTCCGGTGCTGGCCCAGGCGAATACGCTACGCCCGGAAGATGCCGACCGACTGGGAATTTATCGTCAGCATTGCCTCGATAACCTGAAAATTCTGCGTGAAAATCCG                                                                                                        >  SRR19973067.691263/1‑150 (MQ=60)
                                               TAACGCCGCCGTTCCGGTTAAGCTGGTGCATATCAATAAATGTCCGGTGCTGGCCCAGGCGAATACGCTACGCCCGGAAGATGCCGACCGACTGGGAATTTATCGTCAGCATTGCCTCGATAACCTGAAAATTCTGCGTGAAAATCCGCA                                                                                                      >  SRR19973067.490571/1‑150 (MQ=60)
                                               TAACGCCGCCGTTCCGGTTAAGCTGGTGCATATCAATAAATGTCCGGTGCTGGCCCAGGCGAATACGCTACGCCCGGAAGATGCCGACCGACTGGGAATTTATCGTCAGCATTGCCTCGATAACCTGAAAATTCTGCGTGAAAATCCGCA                                                                                                      >  SRR19973067.490575/1‑150 (MQ=60)
                                                   GCCGCCGTTCCGGTTAAGCTGGTGCATATCAATAAATGTCCGGTGCTGGCCCAGGCGAATACGCTACGCCCGGAAGATGCCGACCGACTGGGAATTTATCGTCAGCATTGCCTCGATAACCTGAAAATTCTGCGTGAAAATCCGCAAGTG                                                                                                  <  SRR19973067.3109403/150‑1 (MQ=60)
                                                   GCCGCCGTTCCGGTTAAGCTGGTGCATATCAATAAATGTCCGGTGCTGGCCCAGGCGAATACGCTACGCCCGGAAGATGCCGACCGACTGGGAATTTATCGTCAGCATTGCCTCGATAACCTGAAAATTCTGCGTGAAAATCCGCAAGTG                                                                                                  <  SRR19973067.2350729/150‑1 (MQ=60)
                                                   GCCGCCGTTCCGGTTAAGCTGGTGCATATCAATAAATGTCCGGTGCTGGCCCAGGCGAATACGCTACGCCCGGAAGATGCCGACCGACTGGGAATTTATCGTCAGCATTGCCTCGATAACCTGAAAATTCTGCGTGAAAATCCGCAAGTG                                                                                                  <  SRR19973067.1328419/150‑1 (MQ=60)
                                                   GCCGCCGTTCCGGTTAAGCTGGTGCATATCAATAAATGTCCGGTGCTGGCCCAGGCGAATACGCTACGCCCGGAAGATGCCGACCGACTGGGAATTTATCGTCAGCATTGCCTCGATAACCTGAAAATTCTGCGTGAAAATCCGCAAGTG                                                                                                  <  SRR19973067.1939564/150‑1 (MQ=60)
                                                   GCCGCCGTTCCGGTTAAGCTGGTGCATATCAATAAATGTCCGGTGCTGGCCCAGGCGAATACGCTACGCCCGGAAGATGCCGACCGACTGGGAATTTATCGTCAGCATTGCCTCGATAACCTGAAAATTCTGCGTGAAAATCCGCAAGTG                                                                                                  <  SRR19973067.1939526/150‑1 (MQ=60)
                                                   GCCGCCGTTCCGGTTAAGCTGGTGCATATCAATAAATGTCCGGTGCTGGCCCAGGCGAATACGCTACGCCCGGAAGATGCCGACCGACTGGGAATTTATCGTCAGCATTGCCTCGATAACCTGAAAATTCTGCGTGAAAATCCGCAAGTG                                                                                                  <  SRR19973067.1939556/150‑1 (MQ=60)
                                                     CGCCGTTCCGGTTAAGCTGGTGCATATCAATAAATGTCCGGTGCTGGCCCAGGCGAATACGCTACGCCCGGAAGATGCCGACCGACTGGGAATTTATCGTCAGCATTGCCTCGATAACCTGAAAATTCTGCGTGAAAATCCGCAAGTGCG                                                                                                >  SRR19973067.3110167/1‑150 (MQ=60)
                                                       CCGTTCCGGTTAAGCTGGTGCATATCAATAAATGTCCGGTGCTGGCCCAGGCGAATACGCTACGCCCGGAAGATGCCGACCGACTGGGAATTTATCGTCAGCATTGCCTCGATAACCTGAAAATTCTGCGTGAAAATCCGCAAGTGCGCG                                                                                              <  SRR19973067.1792453/150‑1 (MQ=60)
                                                              GGTTAAGCTGGTGCATATCAATAAATGTCCGGTGCTGGCCCAGGCGAATACGCTACGCCCGGAAGATGCCGACCGACTGGGAATTTATCGTCAGCATTGCCTCGATAACCTGAAAATTCTGCGTGAAAATCCGCAAGTGCGCGAAAAAGT                                                                                       >  SRR19973067.2061853/1‑150 (MQ=60)
                                                              GGTTAAGCTGGTGCATATCAATAAATGTCCGGTGCTGGCCCAGGCGAATACGCTACGCCCGGAAGATGCCGACCGACTGGGAATTAATCGTCAGCATTGCCTCGATAACCTGAAAATTCTGCGTGAAAATCCGCAAGTGCGCGAAAAAGT                                                                                       <  SRR19973067.614529/150‑1 (MQ=60)
                                                               GTTAAGCTGGTGCATATCAATAAATGTCCGGTGCTGGCCCAGGCGAATACGCTACGCCCGGAAGATGCCGACCGACTGGGAATTTATCGTCAGCATTGCCTCGATAACCTGAAAATTCTGCGTGAAAATCCGCAAGTGCGCGAAAAAGTG                                                                                      >  SRR19973067.1891647/1‑150 (MQ=60)
                                                               GTTAAGCTGGTGCATATCAATAAATGTCCGGTGCTGGCCCAGGCGAATACGCTACGCCCGGAAGATGCCGACCGACTGGGAATTTATCGTCAGCATTGCCTCGATAACCTGAAAATTCTGCGTGAAAATCCGCAAGTGCGCGAAAAAGTG                                                                                      >  SRR19973067.1891661/1‑150 (MQ=60)
                                                               GTTAAGCTGGTGCATATCAATAAATGTCCGGTGCTGGCCCAGGCGAATACGCTACGCCCGGAAGATGCCGACCGACTGGGAATTTATCGTCAGCATTGCCTCGATAACCTGAAAATTCTGCGTGAAAATCCGCAAGTGCGCGAAAAAGTG                                                                                      >  SRR19973067.81552/1‑150 (MQ=60)
                                                               GTTAAGCTGGTGCATATCAATAAATGTCCGGTGCTGGCCCAGGCGAATACGCTACGCCCGGAAGATGCCGACCGACTGGGAATTTATCGTCAGCATTGCCTCGATAACCTGAAAATTCTGCGTGAAAATCCGCAAGTGCGCGAAAAAGTG                                                                                      >  SRR19973067.1155173/1‑150 (MQ=60)
                                                                   AGCTGGTGCATATCAATAAATGTCCGGTGCTGGCCCAGGCGAATACGCTACGCCCGGAAGATGCCGACCGACTGGGAATTTATCGTCAGCATTGCCTCGATAACCTGAAAATTCTGCGTGAAAATCCGCAAGTGCGCGAAAAAGTGGTGG                                                                                  <  SRR19973067.2996358/150‑1 (MQ=60)
                                                                      TGGTGCATATCAATAAATGTCCGGTGCTGGCCCAGGCGAATACGCTACGCCCGGAAGATGCCGACCGACTGGGAATTTATCGTCAGCATTGCCTCGATAACCTGAAAATTCTGCGTGAAAATCCGCAAGTGCGCGAAAAAGTGGTGGCGA                                                                               <  SRR19973067.311631/150‑1 (MQ=60)
                                                                        GTGCATATCAATAAAGGTCCGGTGCTGGCCCAGGCGAATACGCTACGCCCGGAAGATGCCGACCGACTGGGAATTTATCGTCAGCATTGCCTCGATAACCTGAAAATTCTGCGTGAAAATCCGCAAGTGCGCGAAAAAGTGGTGGCGATA                                                                             <  SRR19973067.3438445/150‑1 (MQ=60)
                                                                          GCATATCAATAAATGTCCGGTGCTGGCCCAGGCGAATACGCTACGCCCGGAAGATGCCGACCGACTGGGAATTTATCGTCAGCATTGCCTCGATAACCTGAAAATTCTGCGTGAAAATCCGCAAGTGCGCGAAAAAGTGGTGGCGATATT                                                                           >  SRR19973067.2154097/1‑150 (MQ=60)
                                                                           CATATCAATAAATGTCCGGTGCTGGCCCAGGCGAATACGCTACGCCCGGAAGATGCCGACCGACTGGGAATTTATCGTCAGCATTGCCTCGATAACCTGAAAATTCTGCGTGAAAATCCGCAAGTGCGCGAAAAAGTGGTGGCGATATTC                                                                          <  SRR19973067.278835/150‑1 (MQ=60)
                                                                           CATATCAATAAATGTCCGGTGCTGGCCCAGGCGAATACGCTACGCCCGGAAGATGCCGACCGACTGGGAATTTATCGTCAGCATTGCCTCGATAACCTGAAAATTCTGCGTGAAAATCCGCAAGTGCGCGAAAAAGTGGTGGCGATATTC                                                                          <  SRR19973067.523208/150‑1 (MQ=60)
                                                                           CATATCAATAAATGTCCGGTGCTGGCCCAGGCGAATACGCTACGCCCGGAAGATGCCGACCGACTGGGAATTTATCGTCAGCATTGCCTCGATAACCTGAAAATTCTGCGTGAAAATCCGCAAGTGCGCGAAAAAGTGGTGGCGATATTC                                                                          <  SRR19973067.1746720/150‑1 (MQ=60)
                                                                                  ATAAATGTCCGGTGCTGGCCCAGGCGAATACGCTACGCCCGGAAGATGCCGACCGACTGGGAATTTATCGTCAGCATTGCCTCGATAACCTGAAAATTCTGCGTGAAAATCCGCAAGTGCGCGAAAAAGTGGTGGCGATATTCGCGGAAG                                                                   <  SRR19973067.2517650/150‑1 (MQ=60)
                                                                                  ATAAATGTCCGGTGCTGGCCCAGGCGAATACGCTACGCCCGGAAGATGCCGACCGACTGGGAATTTATCGTCAGCATTGCCTCGATAACCTGAAAATTCTGCGTGAAAATCCGCAAGTGCGCGAAAAAGTGGTGGCGATATTCGCGGAAG                                                                   <  SRR19973067.2060916/150‑1 (MQ=60)
                                                                                    AAATGTCCGGTGCTGGCCCAGGCGAATACGCTACGCCCGGAAGATGCCGACCGACTGGGAATTTATCGTCAGCATTGCCTCGATAACCTGAAAATTCTGCGTGAAAATCCGCAAGTGCGCGAAAAAGTGGTGGCGATATTCGCGGAAGCC                                                                 <  SRR19973067.2764038/150‑1 (MQ=60)
                                                                                    AAATGTCCGGTGCTGGCCCAGGCGAATACGCTACGCCCGGAAGATGCCGACCGACTGGGAATTTATCGTCAGCATTGCCTCGATAACCTGAAAATTCTGCGTGAAAATCCGCAAGTGCGCGAAAAAGTGGTGGCGATATTCGCGGAAGCC                                                                 >  SRR19973067.318645/1‑150 (MQ=60)
                                                                                    AAATGTCCGGTGCTGGCCCAGGCGAATACGCTACGCCCGGAAGATGCCGACCGACTGGGAATTTATCGTCAGCATTGCCTCGATAACCTGAAAATTCTGCGTGAAAATCCGCAAGTGCGCGAAAAAGTGGTGGCGATATTCGCGGAAGCC                                                                 >  SRR19973067.318455/1‑150 (MQ=60)
                                                                                          CCGGTGCTGGCCCAGGCGAATACGCTACGCCCGTATGATGCAGACCGACTGGTAATTAATCGTCAGCATTGCCTCGATAACCTGAAAATTCTGCGTGAAAATCCGCAAGTGCGCGAAAAAGTGGTGGCGATATTCGCGGAAGCCGAACCG                                                           <  SRR19973067.750426/150‑1 (MQ=60)
                                                                                            GGTGCTGGCCCAGGCGAATACGCTACGCCCGGAAGATGCCGACCGACTGGGAATTTATCGTCAGCATTGCCTCGATAACCTGAAAATTCTGCGTGAAAATCCGCAAGTGCGCGAAAAAGTGGTGGCGATATTCGCGGAAGCCGAACCGTT                                                         >  SRR19973067.3373077/1‑150 (MQ=60)
                                                                                            GGTGCTGGCCCAGGCGAATACGCTACGCCCGGAAGATGCCGACCGACTGGGAATTTATCGTCAGCATTGCCTCGATAACCTGAAAATTCTGCGTGAAAATCCGCAAGTGCGCGAAAAAGTGGTGGCGATATTCGCGGAAGCCGAACCGTT                                                         <  SRR19973067.2304043/150‑1 (MQ=60)
                                                                                             GTGCTGGCCCAGGCGAATACGCTACGCCCGGAAGATGCCGACCGACTGGGAATTTATCGTCAGCATTGCCTCGATAACCTGAAAATTCTGCGTGAAAATCCGCAAGTGCGCGAAAAAGTGGTGGCGATATTCGCGGAAGCCGAACCGTTT                                                        <  SRR19973067.1182245/150‑1 (MQ=60)
                                                                                              TGCTGGCCCAGGCGAATACGCTACGCCCGGAAGATGCCGACCGACTGGGAATTTATCGTCAGCATTGCCTCGATAACCTGAAAATTCTGCGTGAAAATCCGCAAGTGCGCGAAAAAGTGGTGGCGATATTCGCGGAAGCCGAACCGTTTA                                                       <  SRR19973067.988541/150‑1 (MQ=60)
                                                                                              TGCTGGCCCAGGCGAATACGCTACGCCCGGAAGATGCCGACCGACTGGGAATTTATCGTCAGCATTGCCTCGATAACCTGAAAATTCTGCGTGAAAATCCGCAAGTGCGCGAAAAAGTGGTGGCGATATTCGCGGAAGCCGAACCGTTTA                                                       <  SRR19973067.988516/150‑1 (MQ=60)
                                                                                              TGCTGGCCCAGGCGAATACGCTACGCCCGGAAGATGCCGACCGACTGGGAATTTATCGTCAGCATTGCCTCGATAACCTGAAAATTCTGCGTGAAAATCCGCAAGTGCGCGAAAAAGTGGTGGCGATATTCGCGGAAGCCGAACCGTTTA                                                       >  SRR19973067.3492917/1‑150 (MQ=60)
                                                                                              TGCTGGCCCAGGCGAATACGCTACGCCCGGAAGATGCCGACCGACTGGGAATTTATCGTCAGCATTGCCTCGATAACCTGAAAATTCTGCGTGAAAATCCGCAAGTGCGCGAAAAAGTGGTGGCGATATTCGCGGAAGCCGAACCGTTTA                                                       >  SRR19973067.2381263/1‑150 (MQ=60)
                                                                                              TGCTGGCCCAGGCGAATACGCTACGCCCGGAAGATGCCGACCGACTGGGAATTTATCGTCAGCATTGCCTCGATAACCTGAAAATTCTGCGTGAAAATCCGCAAGTGCGCGAAAAAGTGGTGGCGATATTCGCGGAAGCCGAACCGTTTA                                                       >  SRR19973067.2381392/1‑150 (MQ=60)
                                                                                              TGCTGGCCCAGGCGAATACGCTACGCCCGGAAGATGCCGACCGACTGGGAATTTATCGTCAGCATTGCCTCGATAACCTGAAAATTCTGCGTGAAAATCCGCAAGTGCGCGAAAAAGTGGTGGCGATATTCGCGGAAGCCGAACCGTTTA                                                       >  SRR19973067.2381150/1‑150 (MQ=60)
                                                                                              TGCTGGCCCAGGCGAATACGCTACGCCCGGAAGATGCCGACCGACTGGGAATTTATCGTCAGCATTGCCTCGATAACCTGAAAATTCTGCGTGAAAATCCGCAAGTGCGCGAAAAAGTGGTGGCGATATTCGCGGAAGCCGAACCGTTTA                                                       <  SRR19973067.1377105/150‑1 (MQ=60)
                                                                                              TGCTGGCCCAGGCGAATACGCTACGCCCGGAAGATGCCGACCGACTGGGAATTTATCGTCAGCATTGCCTCGATAACCTGAAAATTCTGCGTGAAAATCCGCAAGTGCGCGAAAAAGTGGTGGCGATATTCGCGGAAGCCGAACCGTTTA                                                       >  SRR19973067.1722576/1‑150 (MQ=60)
                                                                                              TGCTGGCCCAGGCGAATACGCTACGCCCGGAAGATGCCGACCGACTGGGAATTTATCGTCAGCATTGCCTCGATAACCTGAAAATTCTGCGTGAAAATCCGCAAGTGCGCGAAAAAGTGGTGGCGATATTCGCGGAAGCCGAACCGTTTA                                                       >  SRR19973067.1722544/1‑150 (MQ=60)
                                                                                               GCTGGCCCAGGCGAATACGCTACGCCCGGAAGATGCCGACCGACTGGGAATTTATCGTCAGCATTGCCTCGATAACCTGAAAATTCTGCGTGAAAATCCGCAAGTGCGCGAAAAAGTGGTGGCGATATTCGCGGAAGCCGAACCGTTTAC                                                      <  SRR19973067.1608094/150‑1 (MQ=60)
                                                                                               GCTGGCCCAGGCGAATACGCTACGCCCGGAAGATGCCGACCGACTGGGAATTAATCGTCAGCATTGCCTCGATAACCTGAAAATTCTGCGTGAAAATCCGCAAGTGCGCGAAAAAGTGGTGGCGATATTCGCGGAAGCCGAACCGTTTAC                                                      <  SRR19973067.133628/150‑1 (MQ=60)
                                                                                               GCTGGCCCAGGCGAATACGCTACGCCCGGAAGATGCCGACCGACTGGGAATTAATCGTCAGCATTGCCTCGATAACCTGAAAATTCTGCGTGAAAATCCGCAAGTGCGCGAAAAAGTGGTGGCGATATTCGCGGAAGCCGAACCGTTTAC                                                      <  SRR19973067.2285932/150‑1 (MQ=60)
                                                                                                 TGGCCCAGGCGAATACGCTACGCCCGGAAGATGCCGACCGACTGGGAATTTATCGTCAGCATTGCCTCGATAACCTGAAAATTCTGCGTGAAAATCCGCAAGTGCGCGAAAAAGTGGTGGCGATATTCGCGGAAGCCGAACCGTTTACGC                                                    >  SRR19973067.316652/1‑150 (MQ=60)
                                                                                                 TGGCCCAGGCGAATACGCTACGCCCGGAAGATGCCGACCGACTGGGAATTTATCGTCAGCATTGCCTCGATAACCTGAAAATTCTGCGTGAAAATCCGCAAGTGCGCGAAAAAGTGGTGGCGATATTCGCGGAAGCCGAACCGTTTACGC                                                    >  SRR19973067.62177/1‑150 (MQ=60)
                                                                                                    CCCAGGCGAATACGCTACGCCCGGAAGATGCCGACCGACTGGGAATTTATCGTCAGCATTGCCTCGATAACCTGAAAATTCTGCGTGAAAATCCGCAAGTGCGCGAAAAAGTGGTGGCGATATTCGCGGAAGCCGAACCGTTTACGCCTT                                                 <  SRR19973067.1622470/150‑1 (MQ=60)
                                                                                                    CCCAGGCGAATACGCTACGCCCGGAAGATGCCGACCGACTGGGAATTTATCGTCAGCATTGCCTCGATAACCTGAAAATTCTGCGTGAAAATCCGCAAGTGCGCGAAAAAGTGGTGGCGATATTCGCGGAAGCCGAACCGTTTACGCCTT                                                 <  SRR19973067.678259/150‑1 (MQ=60)
                                                                                                    CCCAGGCGAATACGCTACGCCCGGAAGATGCCGACCGACTGGGAATTTATCGTCAGCATTGCCTCGATAACCTGAAAATTCTGCGTGAAAATCCGCAAGTGCGCGAAAAAGTGGTGGCGATATTCGCGGAAGCCGAACCGTTTACGCCTT                                                 <  SRR19973067.1622434/150‑1 (MQ=60)
                                                                                                         GCGAATACGCTACGCCCGGAAGATGCCGACCGACTGGGAATTTATCGTCAGCATTGCCTCGATAACCTGAAAATTCTGCGTGAAAATCCGCAAGTGCGCGAAAAAGTGGTGGCGATATTCGCGGAAGCCGAACCGTTTACGCCTTCAGAT                                            <  SRR19973067.2066674/150‑1 (MQ=60)
                                                                                                             ATACGCTACGCCCGGAAGATGCCGACCGACTGGGAATTTATCGTCAGCATTGCCTCGATAACCTGAAAATTCTGCGTGAAAATCCGCAAGTGCGCGAAAAAGTGGTGGCGATATTCGCGGAAGCCGAACCGTTTACGCCTTCAGATAACG                                        <  SRR19973067.1085925/150‑1 (MQ=60)
                                                                                                                CGCTACGCCCGGAAGATGCCGACCGACTGGGAATTTATCGTCAGCATTGCCTCGATAACCGGAAAATTCTGCGTGAAAATCCGCAAGTGCGCGAAAAAGGGGTGGCGATATTCGCGGAAGCCGAACCGTTTACGCCTTCAGATAACGTGG                                     >  SRR19973067.1626114/1‑150 (MQ=60)
                                                                                                                  CTACGCCCGGAAGATGCCGACCGACTGGGAATTTATCGTCAGCATTGCCTCGATAACCTGAAAATTCTGCGTGAAAATCCGCAAGTGCGCGAAAAAGTGGTGGCGATATTCGCGGAAGCCGAACCGTTTACGCCTTCAGATAACGTGGAT                                   <  SRR19973067.1636654/150‑1 (MQ=60)
                                                                                                                   TACGCCCGGAAGATGCCGACCGACTGGGAATTAATCGTCAGCATTGCCTCGATAACCTGAAAATTCTGCGTGAAAATCCGCAAGTGCGCGAAAAAGTGGTGGCGATATTCGCGGAAGCCGAACCGTTTACGCCTTCAGATAACGTGGATG                                  <  SRR19973067.164853/150‑1 (MQ=60)
                                                                                                                   TACGCCCGGAAGATGCCGACCGACTGGGAATTAATCGTCAGCATTGCCTCGATAACCTGAAAATTCTGCGTGAAAATCCGCAAGTGCGCGAAAAAGTGGTGGCGATATTCGCGGAAGCCGAACCGTTTACGCCTTCAGATAACGTGGATG                                  <  SRR19973067.164909/150‑1 (MQ=60)
                                                                                                                     CGCCCGGAAGATGCCGACCGACTGGGAATTTATCGTCAGCATTGCCTCGATAACCTGAAAATTCTGCGTGAAAATCCGCAAGTGCGCGAAAAAGTGGTGGCGATATTCGCGGAAGCCGAACCGTTTACGCCTTCAGATAACGTGGATGCA                                <  SRR19973067.1137499/150‑1 (MQ=60)
                                                                                                                        CCGGAAGATGCCGACCGACTGGGAATTTATCGTCAGCATTGCCTCGATAACCTGAAAATTCTGCGTGAAAATCCGCAAGTGCGCGAAAAAGTGGTGGCGATATTCGCGGAAGCCGAACCGTTTACGCCTTCAGATAACGTGGATGCACAG                             <  SRR19973067.1445171/150‑1 (MQ=60)
                                                                                                                         CGGAAGATGCCGACCGACTGGGAATTTATCGTCAGCATTGCCTCGATAACCTGAAAATTCTGCGTGAAAATCCGCAAGTGCGCGAAAAAGTGGTGGCGATATTCGCGGAAGCCGAACCGTTTACGCCTTCAGATAACGTGGATGCACAGC                            >  SRR19973067.1852840/1‑150 (MQ=60)
                                                                                                                          GGAAGATGCCGACCGACTGGGAATTTATCGTCAGCATTGCCTCGATAACCTGAAAATTCTGCGTGAAAATCCGCAAGTGCGCGAAAAAGTGGTGGCGATATTCGCGGAAGCCGAACCGTTTACGCCTTCAGATAACGTGGATGCACAGCT                           >  SRR19973067.3522035/1‑150 (MQ=60)
                                                                                                                          GGAAGATGCCGACCGACTGGGAATTTATCGTCAGCATTGCCTCGATAACCTGAAAATTCTGCGTGAAAATCCGCAAGTGCGCGAAAAAGTGGTGGCGATATTCGCGGAAGCCGAACCGTTTACGCCTTCAGATAACGTGGATGCACAGCT                           >  SRR19973067.3522141/1‑150 (MQ=60)
                                                                                                                             AGATGCCGACCGACTGGGAATTAATCGTCAGCATTGCCTCGATAACCTGAAAATTCTGCGTGAAAATCCGCAAGTGCGCGAAAAAGTGGTGGCGATATTCGCGGAAGCCGAACCGTTTACGCCTTCAGATAACGTGGATGCACAGCTTTA                        <  SRR19973067.727759/150‑1 (MQ=60)
                                                                                                                              GATGCCGACCGACTGGGAATTTATCGTCAGCATTGCCTCGATAACCTGAAAATTCTGCGTGAAAATCCGCAAGTGCGCGAAAAAGTGGTGGCGATATTCGCGGAAGCCGAACCGTTTACGCCTTCAGATAACGTGGATGCACAGCTTTAT                       <  SRR19973067.3561970/150‑1 (MQ=60)
                                                                                                                               ATGCCGACCGACTGGGAATTTATCGTCAGCATTGCCTCGATAACCTGAAAATTCTGCGTGAAAATCCGCAAGTGCGCGAAAAAGTGGTGGCGATATTCGCGGAAGCCGAACCGTTTACGCCTTCAGATAACGTGGATGCACAGCTTTATA                      <  SRR19973067.3047152/150‑1 (MQ=60)
                                                                                                                               ATGCCGACCGACTGGGAATTTATCGTCAGCATTGCCTCGATAACCTGAAAATTCTGCGTGAAAATCCGCAAGTGCGCGAAAAAGTGGTGGCGATATTCGCGGAAGCCGAACCGTTTACGCCTTCAGATAACGTGGATGCACAGCTTTATA                      <  SRR19973067.3047218/150‑1 (MQ=60)
                                                                                                                               ATGCCGACCGACTGGGAATTTATCGTCAGCATTGCCTCGATAACCTGAAAATTCTGCGTGAAAATCCGCAAGTGCGCGAAAAAGTGGTGGCGATATTCGCGGAAGCCGAACCGTTTACGCCTTCAGATAACGTGGATGCACAGCTTTATA                      <  SRR19973067.1359927/150‑1 (MQ=60)
                                                                                                                               ATGCCGACCGACTGGGAATTTATCGTCAGCATTGCCTCGATAACCTGAAAATTCTGCGTGAAAATCCGCAAGTGCGCGAAAAAGTGGTGGCGATATTCGCGGAAGCCGAACCGTTTACGCCTTCAGATAACGTGGATGCACAGCTTTATA                      <  SRR19973067.3124556/150‑1 (MQ=60)
                                                                                                                                TGCCGACCGACTGGGAATTTATCGTCAGCATTGCCTCGATAACCTGAAAATTCTGCGTGAAAATCCGCAAGTGCGCGAAAAAGTGGTGGCGATATTCGCGGAAGCCGAACCGTTTACGCCTTCAGATAACGTGGATGCACAGCTTTATAA                     <  SRR19973067.1094359/150‑1 (MQ=60)
                                                                                                                                   CGACCGACTGGGAATTTATCGTCAGCATTGCCTCGATAACCTGAAAATTCTGCGTGAAAATCCGCAAGTGCGCGAAAAAGTGGTGGCGATATTCGCGGAAGCCGAACCGTTTACGCCTTCAGATAACGTGGATGCACAGCTTTATAACGG                  >  SRR19973067.2019009/1‑150 (MQ=60)
                                                                                                                                   CGACCGACTGGGAATTTATCGTCAGCATTGCCTCGATAACCTGAAAATTCTGCGTGAAAATCCGCAAGTGCGCGAAAAAGTGGTGGCGATATTCGCGGAAGCCGAACCGTTTACGCCTTCAGATAACGTGGATGCACAGCTTTATAACGG                  >  SRR19973067.2018933/1‑150 (MQ=60)
                                                                                                                                   CGACCGACTGGGAATTTATCGTCAGCATTGCCTCGATAACCTGAAAATTCTGCGTGAAAATCCGCAAGTGCGCGAAAAAGTGGTGGCGATATTCGCGGAAGCCGAACCGTTTACGCCTTCAGATAACGTGGATGCACAGCTTTATAACGG                  >  SRR19973067.804581/1‑150 (MQ=60)
                                                                                                                                         ACTGGGAATTTATCGTCAGCATTGCCTCGATAACCTGAAAATTCTGCGTGAAAATCCGCAAGTGCGCGAAAAAGTGGTGGCGATATTCGCGGAAGCCGAACCGTTTACGCCTTCAGATAACGTGGATGCACAGCTTTATAACGGCTTTTT            >  SRR19973067.3362335/1‑150 (MQ=60)
                                                                                                                                         ACTGGGAATTTATCGTCAGCATTGCCTCGATAACCTGAAAATTCTGCGTGAAAATCCGCAAGTGCGCGAAAAAGTGGTGGCGATATTCGCGGAAGCCGAACCGTTTACGCCTTCAGATAACGTGGATGCACAGCTTTATAACGGCTTTTT            >  SRR19973067.3446480/1‑150 (MQ=60)
                                                                                                                                            GGGAATTTATCGTCAGCATTGCCTCGATAACCTGAAAATTCTGCGTGAAAATCCGCAAGTGCGCGAAAAAGTGGTGGCGATATTCGCGGAAGCCGAACCGTTTACGCCTTCAGATAACGTGGATGCACAGCTTTATAACGGCTTTTTCAG         <  SRR19973067.662473/150‑1 (MQ=60)
                                                                                                                                            GGGAATTTATCGTCAGCATTGCCTCGATAACCTGAAAATTCTGCGTGAAAATCCGCAAGTGCGCGAAAAAGTGGTGGCGATATTCGCGGAAGCCGAACCGTTTACGCCTTCAGATAACGTGGATGCACAGCTTTATAACGGCTTTTTCAG         <  SRR19973067.1108840/150‑1 (MQ=60)
                                                                                                                                             GGAATTTATCGTCAGCATTGCCTCGATAACCTGAAAATTCTGCGTGAAAATCCGCAAGTGCGCGAAAAAGTGGTGGCGATATTCGCGGAAGCCGAACCGTTTACGCCTTCAGATAACGTGGATGCACAGCTTTATAACGGCTTTTTCAGT        <  SRR19973067.2846659/150‑1 (MQ=60)
                                                                                                                                                ATTTATCGTCAGCATTGCCTCGATAACCTGAAAATTCTGCGTGAAAATCCGCAAGTGCGCGAAAAAGTGGTGGCGATATTCGCGGAAGCCGAACCGTTTACGCCTTCAGATAACGTGGATGCACAGCTTTATAACGGCTTTTTCAGTGAC     >  SRR19973067.1107608/1‑150 (MQ=60)
                                                                                                                                                ATTTATCGTCAGCATTGCCTCGATAACCTGAAAATTCTGCGTGAAAATCCGCAAGTGCGCGAAAAAGTGGTGGCGATATTCGCGGAAGCCGAACCGTTTACGCCTTCAGATAACGTGGATGCACAGCTTTATAACGGCTTTTTCAGTGAC     <  SRR19973067.3492590/150‑1 (MQ=60)
                                                                                                                                                 TTTATCGTCAGCATTGCCTCGATAACCTGAAAATTCTGCGTGAAAATCCGCAAGTGCGCGAAAAAGTGGTGGCGATATTCGCGGAAGCCGAACCGTTTACGCCTTCAGATAACGTGGATGCACAGCTTTATAACGGCTTTTTCAGTGACG    <  SRR19973067.1366435/150‑1 (MQ=60)
                                                                                                                                                   TATCGTCAGCATTGCCTCGATAACCTGAAAATTCTGCGTGAAAATCCGCAAGTGCGCGAAAAAGTGGTGGCGATATTCGCGGAAGCCGAACCGTTTACGCCTTCAGATAACGTGGATGCACAGCTTTATAACGGCTTTTTCAGTGACGCA  >  SRR19973067.657159/1‑150 (MQ=60)
                                                                                                                                                   TATCGTCAGCATTGCCTCGATAACCTGAAAATTCTGCGTGAAAATCCGCAAGTGCGCGAAAAAGTGGTGGCGATATTCGCGGAAGCCGAACCGTTTACGCCTTCAGATAACGTGGATGCACAGCTTTATAAAGGCTTTTTCAGTGACGCA  >  SRR19973067.657174/1‑150 (MQ=60)
                                                                                                                                                   |                                                                                                                                                     
GACACATTGCGCGAGCGTTTATATACCGCAAAAACCGATCTTGGCGATAACGCCGCCGTTCCGGTTAAGCTGGTGCATATCAATAAATGTCCGGTGCTGGCCCAGGCGAATACGCTACGCCCGGAAGATGCCGACCGACTGGGAATTAATCGTCAGCATTGCCTCGATAACCTGAAAATTCTGCGTGAAAATCCGCAAGTGCGCGAAAAAGTGGTGGCGATATTCGCGGAAGCCGAACCGTTTACGCCTTCAGATAACGTGGATGCACAGCTTTATAACGGCTTTTTCAGTGACGCA  >  CP009273/2077065‑2077361

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑