Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F368 I0 R1 1472 214.4 7116750 95.9% 6824963 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,499,568 C→A 100% D182Y (GAC→TAC)  ypdF ← Xaa‑Pro aminopeptidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,499,5680CA95.3% 503.2 / 15.6 170D182Y (GAC→TAC) ypdFXaa‑Pro aminopeptidase
Reads supporting (aligned to +/- strand):  ref base C (5/3);  new base A (74/88);  total (79/91)
Fisher's exact test for biased strand distribution p-value = 4.74e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.86e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 171 total aligned reads displayed.

CCAGCAAGGTGCGCGTCATATCAGAGCAGTAGCCCTGATACAGCGCACCGAAATCGAGAGTGACAAACTCGCCCGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTCAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGCGCTCGCTCATCCCCGCCTGAATAAAGCGGCGAATATGCTCTGCACCGCGATCAGCAATCCCACAGGCGAGGCGGATTTTC  >  CP009273/2499424‑2499710
                                                                                                                                                |                                                                                                                                              
ccAGCAAGGTGCGCGTCATATCAGAGCAGTAGCCCTGATACAGCGCACCGAACTCGAGAGTGACAAACTCGCCCGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTaaaa                                                                                                                                             <  1:649468/148‑1 (MQ=255)
   gCAAGGTGCGCGTCATATCAGAGCAGTAGCCCTGATACAGCGCACCGAAATCGAGAGTGACAAACTCGCCCGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAaga                                                                                                                                          <  2:1902935/148‑1 (MQ=255)
    cAAGGTGCGCGTCATATCAGAGCAGTAGCCCTGATACAGCGCACCGAAATCGAGAGTGACAAACTCGCCCGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAagag                                                                                                                                         >  2:543836/1‑148 (MQ=255)
    cAAGGTGCGCGTCATATCAGAGCAGTAGCCCTGATACAGCGCACCGAAATCGAGAGTGACAAACTCGCCCGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAagag                                                                                                                                         <  2:481448/148‑1 (MQ=255)
    cAAGGTGCGCGTCATATCAGAGCAGTAGCCCTGATACAGCGCACCGAAATCGAGAGTGACAAACTCGCCCGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAagag                                                                                                                                         <  2:481408/148‑1 (MQ=255)
    cAAGGTGCGCGTCATATCAGAGCAGTAGCCCTGATACAGCGCACCGAAATCGAGAGTGACAAACTCGCCCGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAagag                                                                                                                                         >  1:2473824/1‑148 (MQ=255)
    cAAGGTGCGCGTCATATCAGAGCAGTAGCCCTGATACAGCGCACCGAAATCGAGAGTGACAAACTCGCCCGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAagag                                                                                                                                         <  2:2172893/148‑1 (MQ=255)
     aaGGTGCGCGTCATATCAGAGCAGTAGCCCTGATACAGCGCACCGAAATCGAGAGTGACAAACTCGCCCGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAgg                                                                                                                                        <  1:278913/148‑1 (MQ=255)
      aGGTGCGCGTCATATCAGAGCAGTAGCCCTGATACAGCGCACCGAAATCGAGAGTGACAAACTCGCCCGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTCAAAAGAGGc                                                                                                                                       <  2:1047278/148‑1 (MQ=255)
       ggTGCGCGTCATATCAGAGCAGTAGCCCTGATACAGCGCACCGAAATCGAGAGTGACAAACTCGCCCGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCt                                                                                                                                      <  1:469340/148‑1 (MQ=255)
       ggTGCGCGTCATATCAGAGCAGTAGCCCTGATACAGCGCACCGAAATCGAGAGTGACAAACTCGCCCGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCt                                                                                                                                      >  1:816098/1‑148 (MQ=255)
        gTGCGCGTCATATCAGAGCAGTAGCCCTGATACAGCGCACCGAAATCGAGAGTGACAAACTCGCCCGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCtt                                                                                                                                     <  1:2971445/148‑1 (MQ=255)
                  tatCAGAGCAGTAGCCCTGATACAGCGCACCGAAATCGAGAGTGACAAACTCGCCCGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGcc                                                                                                                           <  2:2330429/148‑1 (MQ=255)
                   atCAGAGCAGTAGCCCTGATACAGCGCACCGGAATCGAGAGTGACAAACTCGCCCGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGccc                                                                                                                          <  2:1187655/148‑1 (MQ=255)
                   atCAGAGCAGTAGCCCTGATACAGCGCACCGAAATCGAGAGTGACAAACTCGCCCGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGccc                                                                                                                          >  2:710602/1‑148 (MQ=255)
                   atCAGAGCAGTAGCCCTGATACAGCGCACCGAAATCGAGAGTGACAAACTCGCCCGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGccc                                                                                                                          <  2:2756583/148‑1 (MQ=255)
                      agagCAGTAGCCCTGATACAGCGCACCGAAATCGAGAGTGACAAACTCGCCCGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCctgc                                                                                                                       <  2:3487605/148‑1 (MQ=255)
                       gagCAGTAGCCCTGATACAGCGCACCGAAATCGAGAGTGACAAACTCGCCCGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCctgct                                                                                                                      >  2:2435887/1‑148 (MQ=255)
                       gagCAGTAGCCCTGATACAGCGCACCGAAATCGAGAGTGACAAACTCGCCCGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCctgct                                                                                                                      >  2:2625183/1‑148 (MQ=255)
                             tAGCCCTGATACAGCGCACCGAAATCGAGAGTGACAAACTCGCCCGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCCCTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCa                                                                                                                >  1:642842/1‑148 (MQ=255)
                             tAGCCCTGATACAGCGCACCGAAATCGAGAGTGACAAACTCGCCCGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCa                                                                                                                <  2:2260797/148‑1 (MQ=255)
                             tAGCCCTGATACAGCGCACCGAAATCGAGAGTGACAAACTCGCCCGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCa                                                                                                                >  1:1929684/1‑148 (MQ=255)
                                cccTGATACAGCGCACCGAAATCGAGAGTGACAAACTCGCCCGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATaa                                                                                                             <  1:1550840/148‑1 (MQ=255)
                                  cTGATACAGCGCACCGAAATCGAGAGTGACAAACTCGCCCGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAAc                                                                                                           >  1:2737506/1‑148 (MQ=255)
                                    gATACAGCGCACCGAAATCGAGAGTGACAAACTCGCCCGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCa                                                                                                         <  1:312918/148‑1 (MQ=255)
                                        cAGCGCACCGAAATCGAGAGTGACAAACTCGCCCGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTcc                                                                                                     >  2:2043100/1‑148 (MQ=255)
                                          gcgcACCGAAATCGAGAGTGACAAACTCGCCCGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAg                                                                                                   >  2:182519/1‑148 (MQ=255)
                                            gcACCGAAATCGAGAGTGACAAACTCGCCCGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGtt                                                                                                 <  2:1005828/148‑1 (MQ=255)
                                             cACCGAAATCGAGAGTGACAAACTCGCCCGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTc                                                                                                <  2:3173960/148‑1 (MQ=255)
                                               ccGAAATCGAGAGTGACAAACTCGCCCGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAg                                                                                              <  1:156056/148‑1 (MQ=255)
                                               ccGAAATCGAGAGTGACAAACTCGCCCGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAg                                                                                              <  1:1953377/148‑1 (MQ=255)
                                                    aTCGAGAGTGACAAACTCGCCCGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCt                                                                                         >  2:387186/1‑148 (MQ=255)
                                                     tCGAGAGTGACAAACTCGCCCGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTa                                                                                        >  1:2246356/1‑148 (MQ=255)
                                                      cGAGAGTGACAAACTCGCCCGCTGCAACAATGTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCTACAATGGTTTAAAAAGAGGCTTTTTCGGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTAt                                                                                       <  1:906717/148‑1 (MQ=255)
                                                      cGAGAGTGACAAACTCGCCCGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTAt                                                                                       >  2:1991448/1‑148 (MQ=255)
                                                      cGAGAGTGACAAACTCGCCCGCTGAAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGAATAAACCACTCCAGTTCAGCGGCTAt                                                                                       <  1:3366771/148‑1 (MQ=255)
                                                       gagaGTGACAACCTCGCCCGCTGCAACAATCTTGTCGCTGGCTTGGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGCGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTAtc                                                                                      <  1:3018366/148‑1 (MQ=255)
                                                        agagTGACAAACTCGCCCGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTAtct                                                                                     >  2:2254353/1‑148 (MQ=255)
                                                        agagTGACAAACTCGCCCGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTAtct                                                                                     >  1:2186239/1‑148 (MQ=255)
                                                            tGACAAACTCGCCCGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTcgcg                                                                                 >  1:1746000/1‑148 (MQ=255)
                                                            tGACAAACTCGCCCGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTcgcg                                                                                 >  1:2963667/1‑148 (MQ=255)
                                                              aCAAACTCGCCCGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCAGTGGCGACAATGTTTTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATACACCACTCCAGTTCAGCGGCTATCTCGcgct                                                                               <  1:2417712/148‑1 (MQ=255)
                                                              aCAAACTCGCCCGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGcgct                                                                               >  2:2505394/1‑148 (MQ=255)
                                                              aCAAACTCGCCCGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGcgct                                                                               >  1:1088272/1‑148 (MQ=255)
                                                              aCAAACTCGCCCGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGcgct                                                                               >  1:1088311/1‑148 (MQ=255)
                                                              aCAAACTCGCCCGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGcgct                                                                               >  1:627034/1‑148 (MQ=255)
                                                               cAAACTCGCCCGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGcgctc                                                                              >  2:3404502/1‑148 (MQ=255)
                                                               cAAACTCGCCCGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGcgctc                                                                              >  2:1644330/1‑148 (MQ=255)
                                                                aaaCTCGCCCGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGcgctcg                                                                             <  2:360028/148‑1 (MQ=255)
                                                                 aaCTCGCCCGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGcgctcgc                                                                            <  2:1670783/148‑1 (MQ=255)
                                                                  aCTCGCCCGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGcgctcgct                                                                           >  1:2254994/1‑148 (MQ=255)
                                                                   cTCGCCCGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGCgctcgctc                                                                          <  1:766088/148‑1 (MQ=255)
                                                                   cTCGCCCGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGCgctcgctc                                                                          >  2:208031/1‑148 (MQ=255)
                                                                     cGCCCGCTGCAACAATGTTGTCGGTGGCTTGGCCGTGGGGCAGCGCCCCCCGCCCGCCACTGGCGACATTGTTTTAAAATGAGGCTTTTTCTGGGCCGTGGTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGCGCTCGCTCAt                                                                        <  1:325930/148‑1 (MQ=255)
                                                                     cGCCCGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGCGCTCGCTCAt                                                                        <  1:2819818/148‑1 (MQ=255)
                                                                     cGCCCGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGCGCTCGCTCAt                                                                        <  1:1693658/148‑1 (MQ=255)
                                                                     cGCCCGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTATGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGCGCTCGCTCAt                                                                        >  1:3399029/1‑148 (MQ=255)
                                                                        ccGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGCGCTCGCTCATccc                                                                     <  2:563886/148‑1 (MQ=255)
                                                                         cGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGCGCTCGCTCATcccc                                                                    >  1:2152001/1‑148 (MQ=255)
                                                                          gCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGCGCTCGCTCATCCCCg                                                                   >  2:684171/1‑148 (MQ=255)
                                                                           cTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGCGCTCGCTCATCCCCGc                                                                  >  2:2931215/1‑148 (MQ=255)
                                                                              caacaaTCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCCTAAACCACTCCAGTTCAGCGGCTCTCTCGCGCTCGCTCATCCCCGCCTg                                                               >  2:2132160/1‑148 (MQ=255)
                                                                               aacaaTCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGCGCTCGCTCATCCCCGCCTGa                                                              <  1:1481898/148‑1 (MQ=255)
                                                                                 caaTCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGCGCTCGCTCATCCCCGCCTGAAt                                                            >  1:333369/1‑148 (MQ=255)
                                                                                 caaTCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGCGCTCGCTCATCCCCGCCTGAAt                                                            >  2:641607/1‑148 (MQ=255)
                                                                                   aTCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGCGCTCGCTCATCCCCGCCTGAATaa                                                          <  1:2621023/148‑1 (MQ=255)
                                                                                   aTCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACCCCAGTTCAGCGGCTATCTCGCGCTCGCTCATCCCCGCCTGAATaa                                                          <  1:3364681/148‑1 (MQ=255)
                                                                                     cTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCCCGCCAGCCCCAGGCGACAAAGGTGTAAAAATAGGCTTTTTCTTCGCCCTGCGGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGCGCTCGCTCATCCCCGCCTGAATAAAg                                                        >  1:1214069/1‑148 (MQ=255)
                                                                                     cTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAATAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGCGCTCGCTCATCCCCGCCGGAATAAAg                                                        >  1:3343684/1‑148 (MQ=255)
                                                                                     cTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGCGCTCGCTCATCCCCGCCTGAATAAAg                                                        >  1:3343614/1‑148 (MQ=255)
                                                                                      ttGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGCGCTCGCTCATCCCCGCCTGAATAAAgc                                                       <  2:3513256/148‑1 (MQ=255)
                                                                                            cTGGCTTTGCCGTGCGGCAGCGCCCCCCGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGCGCTCGCTCATCCCCGCCTGAATAAAGCGGCGaa                                                 <  1:3154563/148‑1 (MQ=255)
                                                                                            cTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGCGCTCGCTCATCCCCGCCTGAATAAAGCGGCGaa                                                 >  1:2156813/1‑148 (MQ=255)
                                                                                              ggCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCTCTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGCGCTCGCTCATCCCCGCCTGAATAAAGCGGCGAata                                               <  2:451194/148‑1 (MQ=255)
                                                                                              ggCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGCGCTCGCTCATCCCCGCCTGAATAAAGCGGCGAata                                               <  1:1125123/148‑1 (MQ=255)
                                                                                                cTTTGCCGTGCGGCAGCGCCCCACGCCCGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTCCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGCGCTCGCTCATCCCCGCCTGAATAAAGCGGCGAATATg                                             <  1:372735/148‑1 (MQ=255)
                                                                                                  ttGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGCGCTCGCTCATCCCCGCCTGAATAAAGCGGCGAATATGct                                           <  2:1784002/148‑1 (MQ=255)
                                                                                                        tGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGCGCTCGCTCATCCCCGCCTGAATAAAGCGGCGAATATGCTCTGCAc                                     >  1:2708559/1‑148 (MQ=255)
                                                                                                             cAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTACTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGCGCTCGCTCATCCCCGCCTGAATAAAGCGGCGAATATGCTCTGCACCGCGa                                <  1:2550972/148‑1 (MQ=255)
                                                                                                               gcgcCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGCGCTCGCTCATCCCCGCCTGAATAAAGCGGCGAATATGCTCTGCACCGCGATc                              <  2:3080197/148‑1 (MQ=255)
                                                                                                               gcgcCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGCGCTCGCTCATCCCCGCCTGAATAAAGCGGCGAATATGCTCTGCACCGCGATc                              <  2:2045528/148‑1 (MQ=255)
                                                                                                                      aCGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGCGCTCGCTCATCCCCGCCTGAATAAAGCGGCGAATATGCTCTGCACCGCGATCAGCAATc                       >  2:2381402/1‑148 (MQ=255)
                                                                                                                       cGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGCGCTCGCTCATCCCCGCCTGAATAAAGCGGCGAATATGCTCTTCACCGCGATCAGCAATcc                      <  2:3221570/148‑1 (MQ=255)
                                                                                                                           agccaCTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGCGCTCGCTCATCCCCGCCTGAATAAAGCGGCGAATATGCTCTGCACCGCGATCAGCAATCCcaca                  <  2:1222142/148‑1 (MQ=255)
                                                                                                                           agccaCTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGCGCTCGCTCATCCCCGCCTGAATAAAGCGGCGAATATGCTCTGCACCGCGATCAGCAATCCcaca                  <  2:1222217/148‑1 (MQ=255)
                                                                                                                           agccaCTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGCGCTCGCTCATCCCCGCCTGAATAAAGCGGCGAATATGCTCTGCACCGCGATCAGCAATCCcaca                  <  2:1538237/148‑1 (MQ=255)
                                                                                                                            gccaCTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGCGCTCGCTCATCCCCGCCTGAATAAAGCGGCGAATATGCTCTGCACCGCGATCAGCAATCCCACag                 >  2:2641766/1‑148 (MQ=255)
                                                                                                                               aCTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGCGCTCGCTCATCCCCGCCTGAATAAAGCGGCGAATATGCTCTGCACCGCGATCAGCAATCCCACaggcg              >  1:221642/1‑148 (MQ=255)
                                                                                                                                cTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGCGCTCGCTCATCCCCGCCTGAATAAAGCGGCGAATATGCTCTGCACCGCGATCAGCAATCCCACaggcga             >  2:1060066/1‑148 (MQ=255)
                                                                                                                                 tGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGCGCTCGCTCATCCCCGCCTGAATAAAGCGGCGAATATGCTCTGCACCGCGATCAGCAATCCCACaggcgag            >  2:2500898/1‑148 (MQ=255)
                                                                                                                                       cAATGGTGTCAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGCGCTCGCTCATCCCCGCCTGAATAAAGCGGCGAATATGCTCTGCACCGCGATCAGCAATCCCACAGGCGAGGCGGAt      >  1:1623616/1‑148 (MQ=255)
                                                                                                                                       cAATGGTGTAAACAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGCGCTCGCTCATCCCCGCCTGAATAAAGCGGCGAATATGCTCTGCACCGCGATCAGCAATCCCACAGGCGAGGCGGAt      <  1:1748593/148‑1 (MQ=255)
                                                                                                                                       cAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGCGCTCGCTCATCCCCGCCTGAATAAAGCGGCGAATATGCTCTGCACCGCGATCAGCAATCCCACAGGCGAGGCGGAt      >  2:3456505/1‑148 (MQ=255)
                                                                                                                                       cAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGCGCTCGCTCATCCCCGCCTGAATAAAGCGGCGAATATGCTCTGCACCGCGATCAGCAATCCCACAGGCGAGGCGGAt      <  1:710596/148‑1 (MQ=255)
                                                                                                                                        aaTGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGCGCTCGCTCATCCCCGCCTGAATAAAGCGGCGAATATGCTCTGCACCGCGATCAGCAATCCCACAGGCGAGGCGGAtt     <  2:3283129/148‑1 (MQ=255)
                                                                                                                                         aTGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGCGCTCGCTCATCCCCGCCTGAATAAAGCGGCGAATATGCTCTGCACCGCGATCAGCAATCCCACAGGCGAGGCGGAttt    >  2:2804036/1‑148 (MQ=255)
                                                                                                                                         aTGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGCGCTCGCTCATCCCCGCCTGAATAAAGCGGCGAATATGCTCTGCACCGCGATCAGCAATCCCACAGGCGAGGCGGAttt    >  2:2231099/1‑148 (MQ=255)
                                                                                                                                         aTGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGCGCTCGCTCATCCCCGCCTGAATAAAGCGGCGAATATGCTCTGCACCGCGATCAGCAATCCCACAGGCGAGGCGGAttt    <  1:437303/148‑1 (MQ=255)
                                                                                                                                         aTGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGCGCTCGCTCATCCCCGCCTGAATAAAGCGGCGAATATGCTCTGCACCGCGATCAGCAATCCCACAGGCGAGGCGGAttt    >  2:1182137/1‑148 (MQ=255)
                                                                                                                                          tGGTGTCAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGCGCTCGCTCATCCCCGCCTGAATAAAGCGGCGAATATGCTCTGCACCGCGATCAGCAATCCCACAGGCGAGGCGGAtttt   >  1:3356594/1‑148 (MQ=255)
                                                                                                                                                |                                                                                                                                              
CCAGCAAGGTGCGCGTCATATCAGAGCAGTAGCCCTGATACAGCGCACCGAAATCGAGAGTGACAAACTCGCCCGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTCAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGCGCTCGCTCATCCCCGCCTGAATAAAGCGGCGAATATGCTCTGCACCGCGATCAGCAATCCCACAGGCGAGGCGGATTTTC  >  CP009273/2499424‑2499710

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CCAGCAAGGTGCGCGTCATATCAGAGCAGTAGCCCTGATACAGCGCACCGAAATCGAGAGTGACAAACTCGCCCGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTCAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGCGCTCGCTCATCCCCGCCTGAATAAAGCGGCGAATATGCTCTGCACCGCGATCAGCAATCCCACAGGCGAGGCGGATTTTCTCCAC  >  CP009273/2499424‑2499715
                                                                                                                                                |                                                                                                                                                   
CCAGCAAGGTGCGCGTCATATCAGAGCAGTAGCCCTGATACAGCGCACCGAACTCGAGAGTGACAAACTCGCCCGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAG                                                                                                                                                <  SRR19973067.653872/150‑1 (MQ=60)
  AGCAAGGTGCGCGTCATATCAGAGCAGTAGCCCTGATACAGCGCACCGAAATCGAGAGTGACAAACTCGCCCGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAG                                                                                                                                              >  SRR19973067.2490086/1‑150 (MQ=60)
     AAGGTGCGCGTCATATCAGAGCAGTAGCCCTGATACAGCGCACCGAAATCGAGAGTGACAAACTCGCCCGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCT                                                                                                                                           >  SRR19973067.821693/1‑150 (MQ=60)
     AAGGTGCGCGTCATATCAGAGCAGTAGCCCTGATACAGCGCACCGAAATCGAGAGTGACAAACTCGCCCGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCT                                                                                                                                           >  SRR19973067.3373554/1‑150 (MQ=60)
     AAGGTGCGCGTCATATCAGAGCAGTAGCCCTGATACAGCGCACCGAAATCGAGAGTGACAAACTCGCCCGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCT                                                                                                                                           <  SRR19973067.280755/150‑1 (MQ=60)
       GGTGCGCGTCATATCAGAGCAGTAGCCCTGATACAGCGCACCGAAATCGAGAGTGACAAACTCGCCCGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTT                                                                                                                                         <  SRR19973067.472583/150‑1 (MQ=60)
       GGTGCGCGTCATATCAGAGCAGTAGCCCTGATACAGCGCACCGAAATCGAGAGTGACAAACTCGCCCGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTT                                                                                                                                         <  SRR19973067.472624/150‑1 (MQ=60)
        GTGCGCGTCATATCAGAGCAGTAGCCCTGATACAGCGCACCGAAATCGAGAGTGACAAACTCGCCCGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTT                                                                                                                                        <  SRR19973067.2990238/150‑1 (MQ=60)
        GTGCGCGTCATATCAGAGCAGTAGCCCTGATACAGCGCACCGAAATCGAGAGTGACAAACTCGCCCGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTT                                                                                                                                        <  SRR19973067.2990190/150‑1 (MQ=60)
           CGCGTCATATCAGAGCAGTAGCCCTGATACAGCGCACCGAAATCGAGAGTGACAAACTCGCCCGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCT                                                                                                                                     <  SRR19973067.2210588/150‑1 (MQ=60)
                           ANTAGCCCTGATACAGCGCACCGAAATCGAGAGTGACAAACTCGCCCGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCA                                                                                                                     >  SRR19973067.1942852/1‑150 (MQ=60)
                           AGTAGCCCTGATACAGCGCACCGAAATCGAGAGTGACAAACTCGCCCGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCCCTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCA                                                                                                                     >  SRR19973067.647173/1‑150 (MQ=60)
                           AGTAGCCCTGATACAGCGCACCGAAATCGAGAGTGACAAACTCGCCCGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCA                                                                                                                     >  SRR19973067.2356864/1‑150 (MQ=60)
                           AGTAGCCCTGATACAGCGCACCGAAATCGAGAGTGACAAACTCGCCCGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCA                                                                                                                     >  SRR19973067.2653695/1‑150 (MQ=60)
                              AGCCCTGATACAGCGCACCGAAATCGAGAGTGACAAACTCGCCCGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAA                                                                                                                  >  SRR19973067.1996533/1‑150 (MQ=60)
                                CCCTGATACAGCGCACCGAAATCGAGAGTGACAAACTCGCCCGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAAC                                                                                                                >  SRR19973067.2755188/1‑150 (MQ=60)
                                CCCTGATACAGCGCACCGAAATCGAGAGTGACAAACTCGCCCGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAAC                                                                                                                <  SRR19973067.1561401/150‑1 (MQ=60)
                                CCCTGATACAGCGCACCGAAATCGAGAGTGACAAACTCGCCCGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAAC                                                                                                                >  SRR19973067.2755183/1‑150 (MQ=60)
                                    GATACAGCGCACCGAAATCGAGAGTGACAAACTCGCCCGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACT                                                                                                            <  SRR19973067.314989/150‑1 (MQ=60)
                                         AGCGCACCGAAATCGAGAGTGACAAACTCGCCCGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGT                                                                                                       >  SRR19973067.280854/1‑150 (MQ=60)
                                          GCGCACCGAAATCGAGAGTGACAAACTCGCCCGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTT                                                                                                      <  SRR19973067.1507113/150‑1 (MQ=60)
                                               CCGAAATCGAGAGTGACAAACTCGCCCGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCG                                                                                                 <  SRR19973067.1966669/150‑1 (MQ=60)
                                               CCGAAATCGAGAGTGACAAACTCGCCCGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCG                                                                                                 <  SRR19973067.157367/150‑1 (MQ=60)
                                               CCGAAATCGAGAGTGACAAACTCGCCCGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCG                                                                                                 <  SRR19973067.157310/150‑1 (MQ=60)
                                                   AATCGAGAGTGACAAACTCGCCCGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTA                                                                                             >  SRR19973067.2261277/1‑150 (MQ=60)
                                                      CGAGAGTGACAAACTCGCCCGCTGCAACAATGTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCTACAATGGTTTAAAAAGAGGCTTTTTCGGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCT                                                                                          <  SRR19973067.912716/150‑1 (MQ=60)
                                                      CGAGAGTGACAAACTCGCCCGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCT                                                                                          >  SRR19973067.2200842/1‑150 (MQ=60)
                                                      CGAGAGTGACAAACTCGCCCGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCT                                                                                          <  SRR19973067.3388761/150‑1 (MQ=60)
                                                      CGAGAGTGACAAACTCGCCCGCTGAAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGAATAAACCACTCCAGTTCAGCGGCTATCT                                                                                          <  SRR19973067.3388146/150‑1 (MQ=60)
                                                       GAGAGTGACAACCTCGCCCGCTGCAACAATCTTGTCGCTGGCTTGGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGCGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTC                                                                                         <  SRR19973067.3037581/150‑1 (MQ=60)
                                                          AGTGACAAACTCGCCCGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGCG                                                                                      >  SRR19973067.1757916/1‑150 (MQ=60)
                                                          AGTGACAAACTCGCCCGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGCG                                                                                      >  SRR19973067.2982387/1‑150 (MQ=60)
                                                            TGACAAACTCGCCCGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGCGCT                                                                                    >  SRR19973067.1095745/1‑150 (MQ=60)
                                                            TGACAAACTCGCCCGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGCGCT                                                                                    >  SRR19973067.631204/1‑150 (MQ=60)
                                                            TGACAAACTCGCCCGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGCGCT                                                                                    >  SRR19973067.631731/1‑150 (MQ=60)
                                                            TGACAAACTCGCCCGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGCGCT                                                                                    >  SRR19973067.1095705/1‑150 (MQ=60)
                                                              ACAACCTCGCCCGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCCCGCCAGCCACTGGCGACAATGGTTTAAAAAGAGGCTTTTTCTGCGCCCTGCGGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGCGCTCG                                                                                  <  SRR19973067.914068/150‑1 (MQ=60)
                                                              ACAAACTCGCCCGCTGCAACAATCTTGTCGCTGGCTTTGCGGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGGTGGCGCATACACCACTCCAGTTCAGCGGCTATCTCGCGCTCG                                                                                  <  SRR19973067.2433662/150‑1 (MQ=60)
                                                              ACAAACTCGCCCGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCAGTGGCGACAATGTTTTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATACACCACTCCAGTTCAGCGGCTATCTCGCGCTCG                                                                                  <  SRR19973067.2433545/150‑1 (MQ=60)
                                                                AAACTCGCCCGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGCGCTCGCT                                                                                >  SRR19973067.2269963/1‑150 (MQ=60)
                                                                 AACTCGCCCGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGCGCTCGCTC                                                                               <  SRR19973067.3457049/150‑1 (MQ=60)
                                                                   CTCGCCCGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGCGCTCGCTCAT                                                                             <  SRR19973067.771432/150‑1 (MQ=60)
                                                                   CTCGCCCGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGCGCTCGCTCAT                                                                             <  SRR19973067.771408/150‑1 (MQ=60)
                                                                   CTCGCCCGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGCGCTCGCTCAT                                                                             <  SRR19973067.40744/150‑1 (MQ=60)
                                                                   CTCGCCCGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTATGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGCGCTCGCTCAT                                                                             >  SRR19973067.3420669/1‑150 (MQ=60)
                                                                     CGCCCGCTGCAACAATGTTGTCGGTGGCTTGGCCGTGGGGCAGCGCCCCCCGCCCGCCACTGGCGACATTGTTTTAAAATGAGGCTTTTTCTGGGCCGTGGTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGCGCTCGCTCATCC                                                                           <  SRR19973067.328154/150‑1 (MQ=60)
                                                                     CGCCCGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGCGCTCGCTCATCC                                                                           <  SRR19973067.2837970/150‑1 (MQ=60)
                                                                     CGCCCGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGCGCTCGCTCATCC                                                                           <  SRR19973067.1705315/150‑1 (MQ=60)
                                                                       CCCGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGCGCTCGCTCATCCCC                                                                         >  SRR19973067.2166389/1‑150 (MQ=60)
                                                                           CTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGCGCTCGCTCATCCCCGCCT                                                                     >  SRR19973067.3074528/1‑150 (MQ=60)
                                                                               AACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGCGCTCGCTCATCCCCGCCTGAAT                                                                 <  SRR19973067.1491731/150‑1 (MQ=60)
                                                                               AACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGCGCTCGCTCATCCCCGCCTGAAT                                                                 >  SRR19973067.335647/1‑150 (MQ=60)
                                                                                   ATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCCCGCCAGCCCCAGGCGACAAAGGTGTAAAAATAGGCTTTTTCTTCGCCCTGCGGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGCGCTCGCTCATCCCCGCCTGAATAAAG                                                             >  SRR19973067.1222101/1‑150 (MQ=60)
                                                                                   ATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCCCAGGCGACAAAGGTGTAAAAAGAGGCTTTTTCTTCGCCCAGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGCGCTCGCTCATACCCGCCGGAATAAAT                                                             >  SRR19973067.3364783/1‑150 (MQ=60)
                                                                                   ATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAATAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGCGCTCGCTCATCCCCGCCGGAATAAAG                                                             >  SRR19973067.3364871/1‑150 (MQ=60)
                                                                                   ATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGCGCTCGCTCATCCCCGCCTGAATAAAG                                                             <  SRR19973067.2636743/150‑1 (MQ=60)
                                                                                   ATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGCGCTCGCTCATCCCCGCCTGAATAAAG                                                             <  SRR19973067.2638026/150‑1 (MQ=60)
                                                                                   ATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGCGCTCGCTCATCCCCGCCTGAATAAAG                                                             >  SRR19973067.3364801/1‑150 (MQ=60)
                                                                                   ATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGCGCTCGCTCATCCCCGCCTGAATAAAG                                                             <  SRR19973067.1191380/150‑1 (MQ=60)
                                                                                   ATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACCCCAGTTCAGCGGCTATCTCGCGCTCGCTCATCCCCGCCTGAATAAAG                                                             <  SRR19973067.3386037/150‑1 (MQ=60)
                                                                                          CGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGCGCTCGCTCATCCCCGCCTGAATAAAGCGGCGAA                                                      <  SRR19973067.1129678/150‑1 (MQ=60)
                                                                                          CGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGCGCTCGCTCATCCCCGCCTGAATAAAGCGGCGAA                                                      >  SRR19973067.2171237/1‑150 (MQ=60)
                                                                                            CTGGCTTTGCCGTGCGGCAGCGCCCCCCGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGCGCTCGCTCATCCCCGCCTGAATAAAGCGGCGAATA                                                    <  SRR19973067.3174556/150‑1 (MQ=60)
                                                                                            CTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGCGCTCGCTCATCCCCGCCTGAATAAAGCGGCGAATA                                                    <  SRR19973067.3174543/150‑1 (MQ=60)
                                                                                              GGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGCGCTCGCTCATCCCCGCCTGAATAAAGCGGCGAATATG                                                  <  SRR19973067.1132724/150‑1 (MQ=60)
                                                                                                CTTTGCCGTGCGGCAGCGCCCCACGCCCGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTCCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGCGCTCGCTCATCCCCGCCTGAATAAAGCGGCGAATATGCT                                                <  SRR19973067.375331/150‑1 (MQ=60)
                                                                                                CTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGCGCTCGCTCATCCCCGCCTGAATAAAGCGGCGAATATGCT                                                <  SRR19973067.2177885/150‑1 (MQ=60)
                                                                                                CTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGCGCTCGCTCATCCCCGCCTGAATAAAGCGGCGAATATGCT                                                <  SRR19973067.1666538/150‑1 (MQ=60)
                                                                                                 TTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGCGCTCGCTCATCCCCGCCTGAATAAAGCGGCGAATATGCTC                                               <  SRR19973067.1949891/150‑1 (MQ=60)
                                                                                                   TGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGAAAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGCGCTCGCTCATCCCCGCCTGAATAAAGCGGCGAATATGCTCTG                                             <  SRR19973067.1715933/150‑1 (MQ=60)
                                                                                                   TGCCGGGCGGCAGCGCCCCACGCCAGCCAGTGGCGACAATGGTGTAAAACGAGGGTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGCGCTCGCTCATCCCCGCCTGAATAAAGCGGCGAATATGCTCTG                                             <  SRR19973067.705453/150‑1 (MQ=60)
                                                                                                      CGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGCGCTCGCTCATCCCCGCCTGAATAAAGCGGCGAATATGCTCTGCAC                                          >  SRR19973067.2726001/1‑150 (MQ=60)
                                                                                                             CAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTACTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGCGCTCGCTCATCCCCGCCTGAATAAAGCGGCGAATATGCTCTGCACCGCGATC                                   <  SRR19973067.2567656/150‑1 (MQ=60)
                                                                                                                          CAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGCGCTCGCTCATCCCCGCCTGAATAAAGCGGCGAATATGCTCTGCACCGCGATCAGCAATCCCACAG                      <  SRR19973067.2875773/150‑1 (MQ=60)
                                                                                                                             CCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGCGCTCGCTCATCCCCGCCTGAATAAAGCGGCGAATATGCTCTGCACCGCGATCAGCAATCCCACAGGCG                   >  SRR19973067.223261/1‑150 (MQ=60)
                                                                                                                             CCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGCGCTCGCTCATCCCCGCCTGAATAAAGCGGCGAATATGCTCTGCACCGCGATCAGCAATCCCACAGGCG                   >  SRR19973067.223413/1‑150 (MQ=60)
                                                                                                                                     GACAATGGTGTCAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGCGCTCGCTCATCCCCGCCTGAATAAAGCGGCGAATATGCTCTGCACCGCGATCAGCAATCCCACAGGCGAGGCGGAT           >  SRR19973067.1634756/1‑150 (MQ=60)
                                                                                                                                       CAATGGTGTAAACAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGCGCTCGCTCATCCCCGCCTGAATAAAGCGGCGAATATGCTCTGCACCGCGATCAGCAATCCCACAGGCGAGGCGGATTT         <  SRR19973067.1760516/150‑1 (MQ=60)
                                                                                                                                       CAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGCGCTCGCTCATCCCCGCCTGAATAAAGCGGCGAATATGCTCTGCACCGCGATCAGCAATCCCACAGGCGAGGCGGATTT         <  SRR19973067.1760423/150‑1 (MQ=60)
                                                                                                                                       CAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGCGCTCGCTCATCCCCGCCTGAATAAAGCGGCGAATATGCTCTGCACCGCGATCAGCAATCCCACAGGCGAGGCGGATTT         <  SRR19973067.715522/150‑1 (MQ=60)
                                                                                                                                        AATGGTGTCAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGCGCTCGCTCATCCCCGCCTGAATAAAGCGGCGAATATGCTCTGCACCGCGATCAGCAATCCCACAGGCGAGGCGGATTTT        >  SRR19973067.3377898/1‑150 (MQ=60)
                                                                                                                                        AATGGTGTCAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGCGCTCGCTCATCCCCGCCTGAATAAAGCGGCGAATATGCTCTGCACCGCGATCAGCAATCCCACAGGCGAGGCGGATTTT        >  SRR19973067.3377948/1‑150 (MQ=60)
                                                                                                                                         ATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGCGCTCGCTCATCCCCGCCTGAATAAAGCGGCGAATATGCTCTGCACCGCGATCAGCAATCCCACAGGCGAGGCGGATTTTC       <  SRR19973067.440347/150‑1 (MQ=60)
                                                                                                                                           GGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGCGCTCGCTCATCCCCGCCTGAATAAAGCGGCGAATATGCTCTGCACCGCGATCAGCAATCCCACAGGCGAGGCGGATTTTCTC     <  SRR19973067.3342659/150‑1 (MQ=60)
                                                                                                                                              GTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGCGCTCGCTCATCCCCGCCTGAATAAAGCGGCGAATATGCTCTGCACCGCGATCAGCAATCCCACAGGCGAGGCGGATTTTCTCCAC  >  SRR19973067.301882/1‑150 (MQ=60)
                                                                                                                                                |                                                                                                                                                   
CCAGCAAGGTGCGCGTCATATCAGAGCAGTAGCCCTGATACAGCGCACCGAAATCGAGAGTGACAAACTCGCCCGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTCAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGCGCTCGCTCATCCCCGCCTGAATAAAGCGGCGAATATGCTCTGCACCGCGATCAGCAATCCCACAGGCGAGGCGGATTTTCTCCAC  >  CP009273/2499424‑2499715

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑