Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F368 I0 R1 1472 214.4 7116750 95.9% 6824963 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,633,402 C→A 100% R178L (CGC→CTC)  hisS ← histidyl tRNA synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,633,4020CA98.4% 634.8 / 0.7 202R178L (CGC→CTC) hisShistidyl tRNA synthetase
Reads supporting (aligned to +/- strand):  ref base C (1/3);  new base A (97/101);  total (98/104)
Fisher's exact test for biased strand distribution p-value = 6.22e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 202 total aligned reads displayed.

ACCTAATGCCGGAGCGTCGTTGAGAAGCGCCTGCACTTCCGGATTTTTTGAATCCAGCACGCGCAGCGGGTTAGTGTACATGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGCGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCAGTGAGCATAATCAGTTCAGCGTCGATATCCGGACCTTGCAGACCGAAAACTTCGC  >  CP009273/2633257‑2633546
                                                                                                                                                 |                                                                                                                                                
aCCTAATGCCGGAGCGTCGTTGAGAAGCGCCTGCACTTCCGGATTTTTTGAATCCAGCACGCGCAGCGGGTTAGTGTACATGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAgg                                                                                                                                                <  2:1719846/148‑1 (MQ=255)
    aaTGCCGGAGCGTCGTTGAGAAGCGCCTGCACTTCCGGATTTTTTGAATCCAGCACGCGCAGCGGGTTAGTGTACATGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAAt                                                                                                                                            >  2:2200725/1‑148 (MQ=255)
     aTGCCGGAGCGTCGTTGAGAAGCGCCTGCACTTCCGGATTTTTTGAATCCAGCACGCGCAGCGGGTTAGTGTACATGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAAtt                                                                                                                                           >  2:591437/1‑148 (MQ=255)
      tGCCGGAGCGTCGTTGAGAAGCGCCTGCACTTCCGGATTTTTTGAATCCAGCACGCGCAGCGGGTTAGTGTACATGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTg                                                                                                                                          >  2:783863/1‑148 (MQ=255)
      tGCCGGAGCGTCGTTGAGAAGCGCCTGCACTTCCGGATTTTTTGAATCCAGCACGCGCAGCGGGTTAGTGTACATGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTg                                                                                                                                          >  1:1799829/1‑148 (MQ=255)
       gCCGGAGCGTCTGTGAGAAGCGCCTGCACGTCCGGATTTTTTGAATCCAGCACGCGCAGCGTGTTAGTGTACATGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGTATGCCACCAGCGCTTCTAGGTAATTgg                                                                                                                                         <  2:1921108/148‑1 (MQ=255)
       gCCGGAGCGTCGTTGAGAAGCGCCTGCACTTCCGGATTTTTTGAATCCAGCACGCGCAGCGGGTTAGTGTACATGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTgg                                                                                                                                         <  1:2027288/148‑1 (MQ=255)
       gCCGGAGCGTCGTTGAGAAGCGCCTGCACTTCCGGATTTTTTGAATCCAGCACGCGCAGCGGGTTAGTGTACATGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTgg                                                                                                                                         <  2:2020034/148‑1 (MQ=255)
           gAGCGTCGTTGAGAAGCGCCTGCACTTCCGGATTTTTTGAATCCAGCACGCGCAGCGGGTTAGTGTACATGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGcgcg                                                                                                                                     >  2:525674/1‑148 (MQ=255)
            aGCGTCGTTGAGAAGCGCCTGCACTTCCGGATTTTTTGAATCCAGCACGCGCAGCGGGTTAGTGTACATGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGt                                                                                                                                    >  2:2550375/1‑148 (MQ=255)
              cgtcgtTGAGAAGCGCCTGCACTTCCGGATTTTTTGAATCCAGCACGCGCAGCGGGTTAGTGTACATGCGGCGTTTTCAGTCTTCGTCCAGCTTTTCTTTATTCTGCTCAAGGAATGCCACCACCGCATCGAGGTAATTGGCGCGTGc                                                                                                                                  >  1:539465/1‑148 (MQ=255)
               gtcgtTGAGAAGCGCCTGCACTTCCGGATTTTTTGAATCCAGCACGCGCAGCGGGTTAGTGTACATGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCt                                                                                                                                 <  2:1317597/148‑1 (MQ=255)
               gtcgtTGAGAAGCGCCTGCACTTCCGGATTTTTTGAATCCAGCACGCGCAGCGGGTTAGTGTACATGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCt                                                                                                                                 <  2:2291570/148‑1 (MQ=255)
                tcgtTGAGAAGCGCCTGCACTTCCGGATTTTTTGAATCCAGCACGCGCAGCGGGTTAGTGTACATGCGGCGTTTGCAGTCTTCGTCCAGCGTTTCTTTATTCTGCTCAAGGAATTCCACCAGCGCATCGAGGTTATTGGCGCGTGCtt                                                                                                                                >  1:952126/1‑148 (MQ=255)
                 cgtTGAGAAGCGCCTGCACTTCCGGATTTTTTGAATCCAGCACGCGCAGCGGGTTAGTGTACATGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTc                                                                                                                               >  2:3424397/1‑148 (MQ=255)
                 cgtTGAGAAGCGCCTGCACTTCCGGATTTTTTGAATCCAGCACGCGCAGCGGGTTAGTGTACATGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTc                                                                                                                               >  2:255927/1‑148 (MQ=255)
                   ttGAGAAGCGCCTGCACTTCCGGATTTTTTGAATCCAGCACGCGCAGCGGGTTAGTGTACATGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCa                                                                                                                             <  2:1754199/148‑1 (MQ=255)
                          gcgcCTGCACTTCCGGATTTTTTGAATCCAGCACGCGCAGCGGGTTAGTGTACATGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAAcc                                                                                                                      >  1:3455879/1‑148 (MQ=255)
                              cTGCACTTCCGGATTTTTTGAATCCAGCACGCGCAGCGGGTTAGTGTACATGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATa                                                                                                                  <  2:2258877/148‑1 (MQ=255)
                               tGCACTTCCGGATTTTTTGAATCCAGCACGCGCAGCGGGTTAGTGTACATGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATag                                                                                                                 <  2:3140465/148‑1 (MQ=255)
                                gCACTTCCGGATTTTTTGAATCCAGCACGCGCAGCGGGTTAGTGTACATGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATaga                                                                                                                <  1:539359/148‑1 (MQ=255)
                                   cTTCCGGATTTTTTGAATCCAGCACGCGCAGCGGGTTAGTGTACATGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCCTCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGtt                                                                                                             >  1:1408849/1‑148 (MQ=255)
                                   cTTCCGGATTTTTTGAATCCAGCACGCGCAGCGGGTTAGTGTACATGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGCGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGtt                                                                                                             <  1:2652856/148‑1 (MQ=255)
                                   cTTCCGGATTTTTTGAATCCAGCACGCGCAGCGGGTTAGTGTACATGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGtt                                                                                                             <  2:3521199/148‑1 (MQ=255)
                                   cTTCCGGATTTTTTGAATCCAGCACGCGCAGCGGGTTAGTGTACATGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGtt                                                                                                             >  1:3100420/1‑148 (MQ=255)
                                   cTTCCGGATTTTTTGAATCCAGCACGCGCAGCGGGTTAGTGTACATGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGtt                                                                                                             >  1:3114422/1‑148 (MQ=255)
                                     tCCGGATTTTTTGAATCCAGCACGCGCAGCGGGTTAGTGTACATGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCa                                                                                                           >  1:1904584/1‑148 (MQ=255)
                                        ggATTTTTTGAATCCATCACGCGCAGAGGGTTAGTGTACATGCGGCGTTTGCAGTCTTCGTACAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTTCTTCCAGCGAACCGATAGAGTTCAGCt                                                                                                        <  1:67986/148‑1 (MQ=255)
                                          aTTTTTTGAATCCAGCACGCGCAGCGGGTTAGTGTACATGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCa                                                                                                      <  2:1256089/148‑1 (MQ=255)
                                          aTTTTTTGAATCCAGCACGCGCAGCGGGTTAGTGTACATGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCa                                                                                                      <  1:624851/148‑1 (MQ=255)
                                                  aaTCCAGCACGCGCAGCGGGTTAGTGTACATGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTAc                                                                                              >  1:1617733/1‑148 (MQ=255)
                                                    tCCAGCACGCGCAGCGGGTTAGTGTACATGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGt                                                                                            >  2:741514/1‑148 (MQ=255)
                                                     ccAGCACGCGCAGCGGGTTAGTGTACATGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTg                                                                                           <  2:968906/148‑1 (MQ=255)
                                                      cAGCACGCGCAGCGGGTTAGTGTACATGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGc                                                                                          <  1:806960/148‑1 (MQ=255)
                                                       aGCACGCGCAGCGGGTTAGTGTACATGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCt                                                                                         <  1:3082434/148‑1 (MQ=255)
                                                       aGCACGCGCAGCGGGTTAGTGTACATGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCt                                                                                         <  1:3082333/148‑1 (MQ=255)
                                                            gcgcAGCGGGTTAGTGTACATGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGaa                                                                                    <  2:2750435/148‑1 (MQ=255)
                                                             cgcAGCGGGTTAGTGTACATGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGaaa                                                                                   <  1:604213/148‑1 (MQ=255)
                                                              gcAGCGGGTTAGTGTACATGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAAt                                                                                  <  1:2256828/148‑1 (MQ=255)
                                                                  cGGGTTAGTGTACATGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATTCCCCCCGCGCCTCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGAAAGAGTTCAGCTCAATAGTTACGTGCTCGGAAATAacc                                                                              >  1:1234033/1‑148 (MQ=255)
                                                                  cGGGTTAGTGTACATGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATAccc                                                                              >  1:2529923/1‑148 (MQ=255)
                                                                      ttAGTGTACATGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAgcg                                                                          >  2:352523/1‑148 (MQ=255)
                                                                           gtACTTGCGGAGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCATCTCAAGAGTTACGTGCTCGGTAATACCCAgcgcgcgc                                                                     <  2:1478127/148‑1 (MQ=255)
                                                                           gtACATGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAgcgcgcgc                                                                     <  2:3244663/148‑1 (MQ=255)
                                                                                 gcggcgTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAg                                                               <  1:525674/148‑1 (MQ=255)
                                                                                 gaggcgTTTGCAGTCTTCGTCCAGCTTTTCTTTATTCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAg                                                               >  2:1169419/3‑148 (MQ=255)
                                                                                      gTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGc                                                          <  2:3071218/148‑1 (MQ=255)
                                                                                      gTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGc                                                          <  2:28616/148‑1 (MQ=255)
                                                                                       tttGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCa                                                         >  1:473586/1‑148 (MQ=255)
                                                                                       tttGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCa                                                         >  2:769775/1‑148 (MQ=255)
                                                                                       tttGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCa                                                         >  1:2262258/1‑148 (MQ=255)
                                                                                        ttGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCAg                                                        <  1:2809082/148‑1 (MQ=255)
                                                                                        ttGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCAg                                                        >  2:337049/1‑148 (MQ=255)
                                                                                        ttGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCAg                                                        >  2:2259220/1‑148 (MQ=255)
                                                                                          gCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCAGTg                                                      <  2:1867904/148‑1 (MQ=255)
                                                                                             gTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCAGTGAGc                                                   >  1:1320635/1‑148 (MQ=255)
                                                                                             gTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCAGTGAGc                                                   >  1:586912/1‑148 (MQ=255)
                                                                                               cTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCAGTGAGCAt                                                 <  1:401740/148‑1 (MQ=255)
                                                                                                ttCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCAGTGAGCATa                                                <  1:2751393/148‑1 (MQ=255)
                                                                                                 tCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCTCGCCACCAGCGGGCAGTGAGCATaa                                               >  1:2277413/1‑148 (MQ=255)
                                                                                                 tCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCAGTGAGCATaa                                               >  1:392743/1‑148 (MQ=255)
                                                                                                 tCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCAGTGAGCATaa                                               <  1:3026137/148‑1 (MQ=255)
                                                                                                   gTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCAGTGAGCATAATc                                             <  1:2550375/148‑1 (MQ=255)
                                                                                                    tCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCAGTGAGCATAATCa                                            >  1:2118764/1‑148 (MQ=255)
                                                                                                    tCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCAGTGAGCATAATCa                                            >  2:1692652/1‑148 (MQ=255)
                                                                                                     ccAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCAGTGAGCATAATCAg                                           >  2:2007357/1‑148 (MQ=255)
                                                                                                      cAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCAGTGAGCATAATCAGt                                          >  1:1022859/1‑148 (MQ=255)
                                                                                                       aGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCAGTGAGCATAATCAGtt                                         >  1:1445118/1‑148 (MQ=255)
                                                                                                        gCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCAGTGAGCATAATCAGTTc                                        <  2:685278/148‑1 (MQ=255)
                                                                                                            ttCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCAGTGAGCATAATCAGTTCAGCg                                    <  1:220088/148‑1 (MQ=255)
                                                                                                             tCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCAGTGAGCATAATCAGTTCAGCGt                                   <  2:500911/148‑1 (MQ=255)
                                                                                                                ttATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCAGTGAGCATAATCAGTTCAGCGTCGa                                <  1:171534/148‑1 (MQ=255)
                                                                                                                 tATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCAGTGAGCATAATCAGTTCAGCGTCGat                               <  2:2763766/148‑1 (MQ=255)
                                                                                                                 tATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCAGTGAGCATAATCAGTTCAGCGTCGat                               <  1:591502/148‑1 (MQ=255)
                                                                                                                  aTGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCAGTGAGCATAATCAGTTCAGCGTCGata                              >  1:378006/1‑148 (MQ=255)
                                                                                                                  aTGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCAGTGAGCATAATCAGTTCAGCGTCGata                              >  2:3164933/1‑148 (MQ=255)
                                                                                                                   tgctgcTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCAGTGAGCATAATCAGTTCAGCGTCGatat                             <  2:840882/148‑1 (MQ=255)
                                                                                                                    gctgctCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCAGTGAGCATAATCAGTTCAGCGTCGATATc                            >  1:19077/1‑148 (MQ=255)
                                                                                                                    gctgctCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCAGTGAGCATAATCAGTTCAGCGTCGATATc                            >  2:138895/1‑148 (MQ=255)
                                                                                                                         tCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCAGTGAGCATAATCAGTTCAGCGTCGATATCCGGAc                       <  1:1160540/148‑1 (MQ=255)
                                                                                                                          cAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCAGTGAGCATAATCAGTTCAGCGTCGATATCCGGAcc                      <  2:2529923/148‑1 (MQ=255)
                                                                                                                          cAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCAGTGAGCATAATCAGTTCAGCGTCGATATCCGGAcc                      <  2:496757/148‑1 (MQ=255)
                                                                                                                            aGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCAGTGAGCATAATCAGTTCAGCGTCGATATCCGGACCtt                    >  2:1365717/1‑148 (MQ=255)
                                                                                                                              gAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCAGTGAGCATAATCAGTTCAGCGTCGATATCCGGACCTTGc                  >  1:2658137/1‑148 (MQ=255)
                                                                                                                                 tGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCAGTGAGCATAATCAGTTCAGCGTCGATATCCGGACCTTGCAGa               <  2:2508450/148‑1 (MQ=255)
                                                                                                                                 tGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCAGTGAGCATAATCAGTTCAGCGTCGATATCCGGACCTTGCAGa               >  1:2495367/1‑148 (MQ=255)
                                                                                                                                  gCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCAGTGAGCATAATCAGTTCAGCGTCGATATCCGGACCTTGCAGAc              <  2:2120343/148‑1 (MQ=255)
                                                                                                                                  gCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCAGTGAGCATAATCAGTTCAGCGTCGATATCCGGACCTTGCAGAc              <  2:1076275/148‑1 (MQ=255)
                                                                                                                                      ccaGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCAGTGAGCATAATCAGTTCAGCGTCGATATCCGTACCTTGCAGACCGaa          >  1:330006/1‑148 (MQ=255)
                                                                                                                                      ccaGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCAGTGAGCATAATCAGTTCAGCGTCGATATCCGGACCTTGCAGACCGaa          >  1:1491049/1‑148 (MQ=255)
                                                                                                                                      ccaGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCAGTGAGCATAATCAGTTCAGCGTCGATATCCGGACCTTGCAGACCGaa          >  1:3526408/1‑148 (MQ=255)
                                                                                                                                       caGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCAGTGAGCATAATCAGTTCAGCGTCGATATCCGGACCTTGCAGACCGaaa         >  1:835852/1‑148 (MQ=255)
                                                                                                                                       caGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCAGTGAGCATAATCAGTTCAGCGTCGATATCCGGACCTTGCAGACCGaaa         >  2:417560/1‑148 (MQ=255)
                                                                                                                                       caGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCAGTGAGCATAATCAGTTCAGCGTCGATATCCGGACCTTGCAGACCGaaa         <  2:2933232/148‑1 (MQ=255)
                                                                                                                                       caGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCAGTGAGCATAATCAGTTCAGCGTCGATATCCGGACCTTGCAGACCGaaa         <  2:2909303/148‑1 (MQ=255)
                                                                                                                                       caGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCAGTGAGCATAATCAGTTCAGCGTCGATATCCGGACCTTGCAGACCGaaa         >  2:2182192/1‑148 (MQ=255)
                                                                                                                                          cgcATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCAGTGAGCATAATCAGTTCAGCGTCGATATCCGGACCTTGCAGACCGAAAACt      <  1:1388397/148‑1 (MQ=255)
                                                                                                                                             aTCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCAGTGAGCATAATCAGTTCAGCGTCGATATCCGGACCTTGCAGACCGAAAACTTCg   >  1:2719146/1‑148 (MQ=255)
                                                                                                                                              tCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCAGTGAGCATAATCAGTTCAGCGTCGATATCCGGACCTTGCAGACCGAAAACTTCGc  >  2:1279582/1‑148 (MQ=255)
                                                                                                                                              tCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCAGTGAGCATAATCAGTTCAGCGTCGATATCCGGACCTTGCAGACCGAAAACTTCGc  >  1:177274/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
ACCTAATGCCGGAGCGTCGTTGAGAAGCGCCTGCACTTCCGGATTTTTTGAATCCAGCACGCGCAGCGGGTTAGTGTACATGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGCGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCAGTGAGCATAATCAGTTCAGCGTCGATATCCGGACCTTGCAGACCGAAAACTTCGC  >  CP009273/2633257‑2633546

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GTCACCTAATGCCGGAGCGTCGTTGAGAAGCGCCTGCACTTCCGGATTTTTTGAATCCAGCACGCGCAGCGGGTTAGTGTACATGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGCGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCAGTGAGCATAATCAGTTCAGCGTCGATATCCGGACCTTGCAGACCGAAAACTTCGCAG  >  CP009273/2633254‑2633548
                                                                                                                                                    |                                                                                                                                                  
GTCACCTAATGCCGGAGCGTCGTTGAGAAGCGCCTGCACTTCCGGATTTTTTGAATCCAGCACGCGCAGCGGGTTAGTGTACATGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAG                                                                                                                                                   <  SRR19973067.947354/150‑1 (MQ=60)
   ACCTAATGCCGGAGCGTCGTTGAGAAGCGCCTGCACTTCCGGATTTTTTGAATCCAGCACGCGCAGCGGGTTAGTGTACATGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTA                                                                                                                                                >  SRR19973067.1193628/1‑150 (MQ=60)
   ACCTAATGCCGGAGCGTCGTTGAGAAGCGCCTGCACTTCCGGATTTTTTGAATCCAGCACGCGCAGCGGGTTAGTGTACATGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTA                                                                                                                                                <  SRR19973067.1584777/150‑1 (MQ=60)
       AATGCCGGAGCGTCGTTGAGAAGCGCCTGCACTTCCGGATTTTTTGAATCCAGCACGCGCAGCGGGTTAGTGTACATGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTG                                                                                                                                            >  SRR19973067.1811922/1‑150 (MQ=60)
        ATGCCGGAGCGTCGTTGAGAAGCGCCTGCACTTCCGGATTTTTTGAATCCAGCACGCGCAGCGGGTTAGTGTACATGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGG                                                                                                                                           <  SRR19973067.2274896/150‑1 (MQ=60)
          GCCGGAGCGTCGTTGAGAAGCGCCTGCACTTCCGGATTTTTTGAATCCAGCACGCGCAGCGGGTTAGTGTACATGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCG                                                                                                                                         <  SRR19973067.2040890/150‑1 (MQ=60)
             GGAGCGTCGTTGAGAAGCGCCTGCACTTCCGGATTTTTTGAATCCAGCACGCGCAGCGGGTTAGTGTACATGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAAATGGCGCGT                                                                                                                                      >  SRR19973067.3056978/1‑150 (MQ=60)
               AGCGTCGTTGAGAAGCGCCTGCACTTCCGGATTTTTTGAATCCAGCACGCGCAGCGGGTTAGTGTACATGCGGCGTTTTCAGTCTTCGTCCAGCTTTTCTTTATTCTGCTCAAGGAATGCCACCACCGCATCGAGGTAATTGGCGCGTGC                                                                                                                                    >  SRR19973067.543059/1‑150 (MQ=60)
               AGCGTCGTTGAGAAGCGCCTGCACTTCCGGATTTTTTGAATCCAGCACGCGCAGCGGGTTAGTGTACATGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAAAGCCACCAGCGCATCGAGGTAATTGGCGCGTGC                                                                                                                                    >  SRR19973067.543057/1‑150 (MQ=60)
                 CGTCGTTGAGAAGCGCCTGCACTTCCGGATTTTTTGAATCCAGCACGCGCAGCGGGTTAGTGTACATGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTT                                                                                                                                  <  SRR19973067.613591/150‑1 (MQ=60)
                 CGTCGTTGAGAAGCGCCTGCACTTCCGGATTTTTTGAATCCAGCACGCGCAGCGGGTTAGTGTACATGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTT                                                                                                                                  <  SRR19973067.1476519/150‑1 (MQ=60)
                 CGTCGTTGAGAAGCGCCTGCACTTCCGGATTTTTTGAATCCAGCACGCGCAGCGGGTTAGTGTACATGCGGCGTTTGCAGTCTTCGTCCAGCGTTTCTTTATTCTGCTCAAGGAATTCCACCAGCGCATCGAGGTTATTGGCGCGTGCTT                                                                                                                                  >  SRR19973067.958641/1‑150 (MQ=60)
                   TCGTTGAGAAGCGCCTGCACTTCCGGAGTTTTTGAATCCAGCACGCGCAGCGGGTTAGTGTACATGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCAACAGCGCATCGAGGTAATTGGCGCGTGCTTCC                                                                                                                                <  SRR19973067.2150623/150‑1 (MQ=60)
                     GTTGAGAAGCGCCTGCACTTCCGGATTTTTTGAATCCAGCACGCGCAGCGGGTTAGTGTACATGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAG                                                                                                                              <  SRR19973067.398919/150‑1 (MQ=60)
                         AGAAGCGCCTGCACTTCCGGATTTTTTGAATCCAGCACGCGCAGCGGGTTAGTGTACATGCGGCGTTTGCAGTCTTCGTCCAGCTTTTGTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAA                                                                                                                          >  SRR19973067.379121/1‑150 (MQ=60)
                          GAAGCGCCTGCACTTCCGGATTTTTTGAATCCAGCACGCGCAGCGGGTTAGTGTACATGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTACCAGCGAAC                                                                                                                         >  SRR19973067.2803052/1‑150 (MQ=60)
                           AAGCGCCTGCACTTCCGGATTTTTTGAATCCAGCACGCGCAGCGGGTTAGTGTACATGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACC                                                                                                                        >  SRR19973067.3477859/1‑150 (MQ=60)
                               GCCTGCACTTCCGGATTTTTTGAATCCAGCACGCGCAGCGGGTTAGTGTACATGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCCCATCGAGGTAATTGGCGCGTGCTTCCAGCCAACCGATA                                                                                                                    >  SRR19973067.540356/1‑150 (MQ=60)
                                   GCACTTCCGGATTTTTTGAATCCAGCACGCGCAGCGGGTTAGTGTACATGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGT                                                                                                                <  SRR19973067.542953/150‑1 (MQ=60)
                                    CACTTCCGGATTTTTTGAATCCAGCACGCGCAGCGGGTTAGTGTACATGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCGGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTT                                                                                                               >  SRR19973067.1340864/1‑150 (MQ=60)
                                    CACTTCCGGATTTTTTGAATCCAGCACGCGCAGCGGGTTAGTGTACATGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCCTCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTT                                                                                                               >  SRR19973067.1418391/1‑150 (MQ=60)
                                    CACTTCCGGATTTTTTGAATCCAGCACGCGCAGCGGGTTAGTGTACATGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTT                                                                                                               >  SRR19973067.3120150/1‑150 (MQ=60)
                                    CACTTCCGGATTTTTTGAATCCAGCACGCGCAGCGGGTTAGTGTACATGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTT                                                                                                               >  SRR19973067.3134220/1‑150 (MQ=60)
                                    CACTTCCGGATTTTTTGAATCCAGCACGCGCAGCGGGTTAGTGTACATGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATACAGGTAATTGGCGCGTGCTTCCAGCGAACAGATAGAGTT                                                                                                               >  SRR19973067.3134124/1‑150 (MQ=60)
                                     ACTTCCGGATTTTTTGAATCCAGCACGCGCAGCGGGTTAGTGTACATGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTC                                                                                                              <  SRR19973067.2270480/150‑1 (MQ=60)
                                      CTTCCGGATTTTTTGAATCCAGCACGCGCAGCGGGTTAGTGTACATGCGGCGTTTGCAGTCTTCGTCCAGCTTTTGTTTATGCTGCTCAAGGAAAGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGACCCGATAGAGTTCA                                                                                                             >  SRR19973067.107245/1‑150 (MQ=60)
                                      CTTCCGGATTTTTTGAATCCAGCACGCGCAGCGGGTTAGTGTACATGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGCGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCA                                                                                                             <  SRR19973067.2669954/150‑1 (MQ=60)
                                      CTTCCGGATTTTTTGAATCCAGCACGCGCAGCGGGTTAGTGTACATGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGCGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCA                                                                                                             <  SRR19973067.2669970/150‑1 (MQ=60)
                                      CTTCCGGATTTTTTGAATCCAGCACGCGCAGCGGGTTAGTGTACATGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCA                                                                                                             >  SRR19973067.1917657/1‑150 (MQ=60)
                                      CTTCCGGATTTTTTGAATCCAGCACGCGCAGCGGGTTAGTGTACATGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCA                                                                                                             >  SRR19973067.1917568/1‑150 (MQ=60)
                                       TTCCGGATTTTTTGAATCCAGCACGCGCAGCGGGTTAGTGTACATGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAG                                                                                                            >  SRR19973067.1774774/1‑150 (MQ=60)
                                           GGATTTTTTGAATCCATCACGCGCAGAGGGTTAGTGTACATGCGGCGTTTGCAGTCTTCGTACAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTTCTTCCAGCGAACCGATAGAGTTCAGCTCA                                                                                                        <  SRR19973067.68788/150‑1 (MQ=60)
                                           GGATTTTTTGAATCCAGCACGCGCAGCGGGTTAGTGTACATGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCA                                                                                                        >  SRR19973067.390506/1‑150 (MQ=60)
                                             ATTTTTTGAATCCAGCACGCGCAGCGGGTTAGTGTACATGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAG                                                                                                      <  SRR19973067.3221936/150‑1 (MQ=60)
                                             ATTTTTTGAATCCAGCACGCGCAGCGGGTTAGTGTACATGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAG                                                                                                      <  SRR19973067.3221909/150‑1 (MQ=60)
                                             ATTTTTTGAATCCAGCACGCGCAGCGGGTTAGTGTACATGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAG                                                                                                      <  SRR19973067.628977/150‑1 (MQ=60)
                                                 TTTGAATCCAGCACGCGCAGCGGGTTAGTGTACATGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTT                                                                                                  <  SRR19973067.482844/150‑1 (MQ=60)
                                                   TGAATCCAGCACGCGCAGCGGGTTAGTGTACATGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGCGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTAC                                                                                                >  SRR19973067.1628857/1‑150 (MQ=60)
                                                   TGAATCCAGCACGCGCAGCGGGTTAGTGTACATGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTAC                                                                                                >  SRR19973067.1628838/1‑150 (MQ=60)
                                                   TGAATCCAGCACGCGCAGCGGGTTAGTGTACATGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTAC                                                                                                <  SRR19973067.460389/150‑1 (MQ=60)
                                                      ATCCAGCACGCGCAGCGGGTTAGTGTACATGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTG                                                                                             >  SRR19973067.3134823/1‑150 (MQ=60)
                                                         CAGCACGCGCAGCGGGTTAGTGTACATGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTC                                                                                          <  SRR19973067.812517/150‑1 (MQ=60)
                                                         CAGCACGCGCAGCGGGTTAGTGTACATGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTC                                                                                          <  SRR19973067.812508/150‑1 (MQ=60)
                                                          AGCACGCGCAGCGGGTTAGTGTACATGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCG                                                                                         <  SRR19973067.953357/150‑1 (MQ=60)
                                                          AGCACGCGCAGCGGGTTAGTGTACATGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCG                                                                                         <  SRR19973067.3102121/150‑1 (MQ=60)
                                                          AGCACGCGCAGCGGGTTAGTGTACATGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCG                                                                                         <  SRR19973067.3102048/150‑1 (MQ=60)
                                                          AGCACGCGCAGCGGGTTAGTGTACATGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCG                                                                                         <  SRR19973067.3102037/150‑1 (MQ=60)
                                                          AGCACGCGCAGCGGGTTAGTGTACATGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCG                                                                                         <  SRR19973067.3101947/150‑1 (MQ=60)
                                                                CGCAGCGGGTTAGTGTACATGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATA                                                                                   <  SRR19973067.608057/150‑1 (MQ=60)
                                                                CGCAGCGGGTTAGTGTACATGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATA                                                                                   <  SRR19973067.608044/150‑1 (MQ=60)
                                                                 GCAGCGGGTTAGTGTACATGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATAC                                                                                  <  SRR19973067.2271807/150‑1 (MQ=60)
                                                                   AGCGGGTTAGTGTACATGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATTCTGCTCAAGGAATTCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCC                                                                                >  SRR19973067.2546428/1‑150 (MQ=60)
                                                                   AGCGGGTTAGTGTACATGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATTCCCCCCGCGCCTCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGAAAGAGTTCAGCTCAATAGTTACGTGCTCGGAAATAACC                                                                                >  SRR19973067.1242307/1‑150 (MQ=60)
                                                                   AGCGGGTTAGTGTACATGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCC                                                                                >  SRR19973067.2546499/1‑150 (MQ=60)
                                                                       GGTTAGTGTACATGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCG                                                                            <  SRR19973067.2421242/150‑1 (MQ=60)
                                                                                    GCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCG                                                               <  SRR19973067.529214/150‑1 (MQ=60)
                                                                                      GGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGG                                                             <  SRR19973067.1953377/150‑1 (MQ=60)
                                                                                        CGTTTGGAGTCTTAGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCA                                                           <  SRR19973067.1467/150‑1 (MQ=60)
                                                                                        CGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCA                                                           >  SRR19973067.476858/1‑150 (MQ=60)
                                                                                        CGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCA                                                           >  SRR19973067.2277262/1‑150 (MQ=60)
                                                                                           TTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCAGTG                                                        <  SRR19973067.2827189/150‑1 (MQ=60)
                                                                                              CAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCAGTGAGC                                                     >  SRR19973067.590662/1‑150 (MQ=60)
                                                                                              CAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCAGTGAGC                                                     >  SRR19973067.590216/1‑150 (MQ=60)
                                                                                              CAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCAGTGAGC                                                     >  SRR19973067.1329651/1‑150 (MQ=60)
                                                                                                  CTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCTCGCCACCAGCGGGCAGTGAGCATAA                                                 >  SRR19973067.2292479/1‑150 (MQ=60)
                                                                                                  CTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCAGTGAGCATAA                                                 <  SRR19973067.404543/150‑1 (MQ=60)
                                                                                                  CTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCAGTGAGCATAA                                                 >  SRR19973067.395491/1‑150 (MQ=60)
                                                                                                   TTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCAGTGAGCATAAT                                                <  SRR19973067.2769175/150‑1 (MQ=60)
                                                                                                   TTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCAGTGAGCATAAT                                                <  SRR19973067.789285/150‑1 (MQ=60)
                                                                                                    TCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCAGTGAGCATAATC                                               <  SRR19973067.3045407/150‑1 (MQ=60)
                                                                                                     CGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCAGTGAGCATAATCA                                              >  SRR19973067.2132892/1‑150 (MQ=60)
                                                                                                      GTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCAGTGAGCATAATCAG                                             <  SRR19973067.2567056/150‑1 (MQ=60)
                                                                                                      GTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCAGTGAGCATAATCAG                                             <  SRR19973067.2567028/150‑1 (MQ=60)
                                                                                                       TCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCAGTGAGCATAATCAGT                                            >  SRR19973067.1029891/1‑150 (MQ=60)
                                                                                                        CCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCAGTGAGCATAATCAGTT                                           >  SRR19973067.663291/1‑150 (MQ=60)
                                                                                                        CCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCAGTGAGCATAATCAGTT                                           >  SRR19973067.264368/1‑150 (MQ=60)
                                                                                                        CCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCAGTGAGCATAATCAGTT                                           >  SRR19973067.1454821/1‑150 (MQ=60)
                                                                                                           GCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCAGTGAGCATAATCAGTTCAG                                        <  SRR19973067.1806772/150‑1 (MQ=60)
                                                                                                               TTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCAGTGAGCATAATCAGTTCAGCGTC                                    <  SRR19973067.221698/150‑1 (MQ=60)
                                                                                                                   TTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCAGTGAGCATAATCAGTTCAGCGTCGATA                                <  SRR19973067.172924/150‑1 (MQ=60)
                                                                                                                   TTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCAGTGAGCATAATCAGTTCAGCGTCGATA                                >  SRR19973067.380634/1‑150 (MQ=60)
                                                                                                                    TATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCAGTGAGCATAATCAGTTCAGCGTCGATAT                               <  SRR19973067.595265/150‑1 (MQ=60)
                                                                                                                    TATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCAGTGAGCATAATCAGTTCAGCGTCGATAT                               <  SRR19973067.595200/150‑1 (MQ=60)
                                                                                                                     ATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCAGTGAGCATAATCAGTTCAGCGTCGATATC                              >  SRR19973067.19372/1‑150 (MQ=60)
                                                                                                                      TGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCAGTGAGCATAATCAGTTCAGCGTCGATATCC                             <  SRR19973067.930022/150‑1 (MQ=60)
                                                                                                                            TCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCAGTGAGCATAATCAGTTCAGCGTCGATATCCGGACCT                       <  SRR19973067.1168267/150‑1 (MQ=60)
                                                                                                                               AGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCAGTGAGCATAATCAGTTCAGCGTCGATATCCGGACCTTGC                    >  SRR19973067.2675245/1‑150 (MQ=60)
                                                                                                                               AGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCAGTGAGCATAATCAGTTCAGCGTCGATATCCGGACCTTGC                    >  SRR19973067.1306765/1‑150 (MQ=60)
                                                                                                                               AGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCAGTGAGCATAATCAGTTCAGCGTCGATATCCGGACCTTGC                    >  SRR19973067.2675777/1‑150 (MQ=60)
                                                                                                                                  AATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCAGTGAGCATAATCAGTTCAGCGTCGATATCCGGACCTTGCAGA                 >  SRR19973067.2511757/1‑150 (MQ=60)
                                                                                                                                   ATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCAGTGAGCATAATCAGTTCAGCGTCGATATCCGGACCTTGCAGAC                <  SRR19973067.3019024/150‑1 (MQ=60)
                                                                                                                                       CACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCAGTGAGCATAATCAGTTCAGCGTCGATATCCGTACCTTGCAGACCGAA            >  SRR19973067.333174/1‑150 (MQ=60)
                                                                                                                                       CACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCAGTGAGCATAATCAGTTCAGCGTCGATATCCGTACCTTGCAGACCGAA            >  SRR19973067.332258/1‑150 (MQ=60)
                                                                                                                                       CACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCAGTGAGCATAATCAGTTCAGCGTCGATATCCGGACCTTGCAGACCGAA            >  SRR19973067.1500913/1‑150 (MQ=60)
                                                                                                                                       CACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCAGTGAGCATAATCAGTTCAGCGTCGATATCCGGACCTTGCAGACCGAA            >  SRR19973067.3548712/1‑150 (MQ=60)
                                                                                                                                       CACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCAGTGAGCATAATCAGTTCAGCGTCGATATCCGGACCTTGCAGACCGAA            >  SRR19973067.3547430/1‑150 (MQ=60)
                                                                                                                                        ACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCAGTGAGCATAATCAGTTCAGCGTCGATATCCGGACCTTGCAGACCGAAA           >  SRR19973067.841534/1‑150 (MQ=60)
                                                                                                                                         CCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCAGTGAGCATAATCAGTTCAGCGTCGATATCCGGACCTTGCAGACCGAAAA          <  SRR19973067.1898399/150‑1 (MQ=60)
                                                                                                                                             CGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCAGTGAGCATAATCAGTTCAGCGTCGATATCCGGACCTTGCAGACCGAAAACTTC      <  SRR19973067.1397837/150‑1 (MQ=60)
                                                                                                                                             CGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCAGTGAGCATAATCAGTTCAGCGTCGATATCCGGACCTTGCAGACCGAAAACTTC      <  SRR19973067.2283513/150‑1 (MQ=60)
                                                                                                                                              GCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCAGTGAGCATAATCAGTTCAGCGTCGATATCCGGACCTTGCAGACCGAAAACTTCG     >  SRR19973067.2737353/1‑150 (MQ=60)
                                                                                                                                              GCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCAGTGAGCATAATCAGTTCAGCGTCGATATCCGGACCTTGCAGACCGAAAACTTCG     >  SRR19973067.2736676/1‑150 (MQ=60)
                                                                                                                                               CATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCAGTGAGCATAATCAGTTCAGCGTCGATATCCGGACCTTGCAGACCGAAAACTTCGC    >  SRR19973067.178691/1‑150 (MQ=60)
                                                                                                                                                ATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCAGTGAGCATAATCAGTTCAGCGTCGATATCCGGACCTTGCAGACCGAAAACTTCGCA   >  SRR19973067.342292/1‑150 (MQ=60)
                                                                                                                                                 TCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCAGTGAGCATAATCAGTTCAGCGTCGATATCCGGACCTTGCAGACCGAAAACTTCGCAG  <  SRR19973067.1412327/150‑1 (MQ=60)
                                                                                                                                                    |                                                                                                                                                  
GTCACCTAATGCCGGAGCGTCGTTGAGAAGCGCCTGCACTTCCGGATTTTTTGAATCCAGCACGCGCAGCGGGTTAGTGTACATGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGCGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCAGTGAGCATAATCAGTTCAGCGTCGATATCCGGACCTTGCAGACCGAAAACTTCGCAG  >  CP009273/2633254‑2633548

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑