Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F368 I0 R1 1472 214.4 7116750 95.9% 6824963 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,041,321 G→T 100% I360I (ATC→ATA)  gcvP ← glycine decarboxylase, PLP‑dependent, subunit (protein P) of glycine cleavage complex

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,041,3210GT95.2% 682.3 / 22.2 230I360I (ATC→ATA) gcvPglycine decarboxylase, PLP‑dependent, subunit (protein P) of glycine cleavage complex
Reads supporting (aligned to +/- strand):  ref base G (3/8);  new base T (109/110);  total (112/118)
Fisher's exact test for biased strand distribution p-value = 2.17e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 230 total aligned reads displayed.

CCGCTTCGGCACGCGTCAGTACGCCCGCTTTGTCGGCCACTTCCACACACAAGGTGTCGAAATAGTGCGCATGGCGCAGTTTCAGACCTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCGATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGTACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTGCTCGCGAGTCTGCATCGCCATGCGCAGCGC  >  CP009273/3041179‑3041464
                                                                                                                                              |                                                                                                                                               
 cGCTTCGGCACGCGTCAGTACGCCCGCTTTGTCGGCCACTTCCACACACAAGGTGTCGAAATAGTGCGCATGGCGCAGTTTCAGACCTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCTATACGt                                                                                                                                           <  2:2676484/148‑1 (MQ=255)
 cGCTTCGGCACGCGTCAGTACGCCCGCTTTGTCGGCCACTTCCACACACAAGGTGTCGAAATAGTGCGCATGGCGCAGTTTCAGACCTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCTATACGt                                                                                                                                           <  2:376465/148‑1 (MQ=255)
 cGCTTCGGCACGCGTCAGTACGCCCGCTTTGTCGGCCACTTCCACACACAAGGTGTCGAAATAGTGCGCATGGCGCAGTTTCAGACCTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCTATACGt                                                                                                                                           <  2:376290/148‑1 (MQ=255)
  gCTTCGGCACGCGTCAGTACGCCCGCTTTGTCGGCCACTTCCACACACAAGGTGTCGAAATAGTGCGCATGGCGCAGTTTCAGACCTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCTCTACGtt                                                                                                                                          >  2:2393229/1‑148 (MQ=255)
   cTTCGGCACGCGTCAGTACGCCCGCTTTGTCGGCCACTTCCACACACAAGGTGTCGAAATAGTGCGCATGGCGCAGTTTCAGACCTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCTATACGttt                                                                                                                                         <  2:3108384/148‑1 (MQ=255)
    ttCGGCACGCGTCAGTACGCCCGCTTTGTCGGCCACTTCCACACACAAGGTGTCGAAATAGTGCGCATGGCGCAGTTTCAGACCTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTc                                                                                                                                        >  2:3417082/1‑148 (MQ=255)
        gCACGCGTCAGTACGCCCGCTTTGTCGGCCACTTCCACACACAAGGTGTCGAAATAGTGCGCATGGCGCAGTTTCAGACCTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGc                                                                                                                                    <  1:429434/148‑1 (MQ=255)
         cACGCGTCAGTACGCCCGCTTTGTCGGCCACTTCCACACACAAGGTGTCGAAATAGTGCGCATGGCGCAGTTTCAGACCTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGcc                                                                                                                                   >  2:2592819/1‑148 (MQ=255)
           cgcgTCAGTACGCCCGCTTTGTCGGCCACTTCCACACACAAGGTGTCGAAATAGTGCGCATGGCGCAGTTTCAGACCTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCaa                                                                                                                                 <  1:3120228/148‑1 (MQ=255)
               tCAGTACGCCCGCTTTGTCGGCCACTTCCACACACAAGGTGTCGAAATAGTGCGCATGGCGCAGTTTCAGACCTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCgg                                                                                                                             <  1:420365/148‑1 (MQ=255)
               tCAGTACGCCCGCTTTGTCGGCCACTTCCACACACAAGGTGTCGAAATAGTGCGCATGGCGCAGTTTCAGACCTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCgg                                                                                                                             <  1:2579844/148‑1 (MQ=255)
                  gTACGCCCGCTTTGTCGGCCACTTCCACACACAAGGTGTCGAAATAGTGCGCATGGCGCAGTTTCAGACCTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCGATACGTTTCAGGCCAACCGGGcc                                                                                                                          >  2:1794159/1‑148 (MQ=255)
                     cGCCCGCTTTGTCGGCCACTTCCACACACAAGGTGTCGAAATAGTGCGCATGGCGCAGTTTCAGACCTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTg                                                                                                                       <  1:2346684/148‑1 (MQ=255)
                      gCCCGCTTTGTCGGCCACTTCCACACACAAGGTGTCGAAATAGTGCGCATGGCGCAGTTTCAGACCTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGa                                                                                                                      >  1:1606764/1‑148 (MQ=255)
                       cccGCTTTGTCGGCCACTTCCACACACAAGGTGTCGAAATAGTGCGCATGGCGCAGTTTCAGACCTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCGATACGTTTCAGGCCAACCGGGCCGTGAt                                                                                                                     <  2:3185462/148‑1 (MQ=255)
                        ccGCTTTGTCGGCCACTTCCACACACAAGGTGTCGAAATAGTGCGCATGGCGCAGTTTCAGACCTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATa                                                                                                                    <  2:1887151/148‑1 (MQ=255)
                        ccGCTTTGTCGGCCACTTCCACACACAAGGTGTCGAAATAGTGCGCATGGCGCAGTTTCAGACCTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATa                                                                                                                    >  2:1167337/1‑148 (MQ=255)
                        ccGCTTTGTCGGCCACTTCCACACACAAGGTGTCGAAATAGTGCGCATGGCGCAGTTTCAGACCTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATa                                                                                                                    >  1:3151182/1‑148 (MQ=255)
                        ccGCTTTGTCGGCCACTTCCACACACAAGGTGTCGAAATAGTGCGCATGGCGCAGTTTCAGACCTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATa                                                                                                                    >  1:3148752/1‑148 (MQ=255)
                         cGCTTTGTCGGCCACTTCCACACACAAGGTGTCGAAATAGTGCGCATGGCGCAGTTTCAGACCTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATaa                                                                                                                   <  1:3062245/148‑1 (MQ=255)
                          gCTTTGTCGGCCACTTCCACACCCAAGGTGTCGAAATAGTGCGCATGGCGCAGTTTCAGACCTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATaaa                                                                                                                  <  2:1972808/148‑1 (MQ=255)
                          gCTTTGTCGGCCACTTCCACACACAAGGTGTCGAAATAGTGCGCATGGCGCAGTTTCAGACCTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCGATACGTTTCAGGCCAACCGGGCCGTGATaaa                                                                                                                  <  1:871791/148‑1 (MQ=255)
                            tttGTCGGCCACTTCCACACACAAGGTGTCGAAATAGTGCGCATGGCGCAGTTTCAGACCTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATAAACg                                                                                                                >  2:1987936/1‑148 (MQ=255)
                            tttGTCGGCCACTTCCACACACAAGGTGTCGAAATAGTGCGCATGGCGCAGTTTCAGACCTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATAAACg                                                                                                                >  1:1172556/1‑148 (MQ=255)
                             ttGTCGGCCACTTCCACACACAAGGTGTCGAAATAGTGCGCATGGCGAAGTTTCATACCTTTTTGTAGAAGGACCGACGCCAGGATATCGGACAGACGGTGAATGAGGTTCGCTATACGTTTCAGTCCAAAAGGGACGAGATAAACgg                                                                                                               >  1:3419874/1‑148 (MQ=255)
                               gTCGGCCACTTCCACACACAAGGTGTCGAAATAGTGCGCATGGCGCAGTTTCAGACCTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCa                                                                                                             <  2:1192723/148‑1 (MQ=255)
                                  ggCCACTTCCACACACAAGGTGTCGAAATAGTGCGCATGGCGCAGTTTCAGACCTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATAc                                                                                                          <  1:2490403/148‑1 (MQ=255)
                                  ggCCACTTCCACACACAAGGTGTCGAAATAGTGCGCATGGCGCAGTTTCAGACCTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATAc                                                                                                          <  1:2490369/148‑1 (MQ=255)
                                   gCCACTTCCACACACAAGGTGTCGAAATAGTGCGCATGGCGCAGTTTCAGACCTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACa                                                                                                         >  2:2455529/1‑148 (MQ=255)
                                   gCCACTTCCACACACAAGGTGTCGAAATAGTGCGCATGGCGCAGTTTCAGACCTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACa                                                                                                         >  1:21807/1‑148 (MQ=255)
                                   gCCACTTCCACACACAAGGTGTCGAAATAGTGCGCATGGCGCAGTTTCAGACCTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACa                                                                                                         >  1:1823288/1‑148 (MQ=255)
                                    ccACTTCCACACACAAGGTGTCGAAATAGTGCGCATGGCGCAGTTTCAGACCTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAg                                                                                                        >  1:97961/1‑148 (MQ=255)
                                     cACTTCCACACACAAGGTGTCGAAATAGTGCGCATGGCGCAGTTTCAGACCTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAgg                                                                                                       <  2:3205016/148‑1 (MQ=255)
                                     cACTTCCACACACAAGGTGTCGAAATAGTGCGCATGGCGCAGTTTCAGACCTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAgg                                                                                                       >  2:2867195/1‑148 (MQ=255)
                                      aCTTCCACACACAAGGTGTCGAAATAGTGCGCATGGCGCAGTTTCAGACCTTTTTGGTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGc                                                                                                      <  1:3285530/148‑1 (MQ=255)
                                       cTTCCACACACAAGGTGTCGAAATAGTGCGCATGGCGCAGTTTCAGACCTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCt                                                                                                     <  2:2210138/148‑1 (MQ=255)
                                         tCCACACACAAGGTGTCGAAATAGTGCGCATGGCGCAGTTTCAGACCTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTgg                                                                                                   <  1:3384027/148‑1 (MQ=255)
                                         tCCACACACAAGGTGTCGAAATAGTGCGCATGGCGCAGTTTCAGACCTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTgg                                                                                                   >  1:3357439/1‑148 (MQ=255)
                                           cacacacaAGGTGTCGAAATAGTGCGCATGGCGCAGTTTCAGACCTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCg                                                                                                 <  2:2241368/148‑1 (MQ=255)
                                           cacacacaAGGTGTCGAAATAGTGCGCATGGCGCAGTTTCAGACCTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCg                                                                                                 <  2:1456521/148‑1 (MQ=255)
                                             cacacaAGGTGTCGAAATAGTGCGCATGGCGCAGTTTCAGACCTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGAt                                                                                               >  2:2335970/1‑148 (MQ=255)
                                               cacaAGGTGTCGAAATAGTGCGCATGGCGCAGTTTCAGACCTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGt                                                                                             <  1:752405/148‑1 (MQ=255)
                                               cacaAGGTGTCGAAATAGTGCGCATGGCGCAGTTTCAGACCTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGt                                                                                             <  1:930220/148‑1 (MQ=255)
                                                acaAGGTGTCGAAATAGTGCGCATGGCGCAGTTTCAGACCTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGtt                                                                                            <  1:1882776/148‑1 (MQ=255)
                                                acaAGGTGTCGAAATAGTGCGCATGGCGCAGTTTCAGACCTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGtt                                                                                            >  2:110139/1‑148 (MQ=255)
                                                     gtgtCGAAATAGTGCGCATGGCGCAGTTTCAGACCTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCca                                                                                       >  1:2507799/1‑148 (MQ=255)
                                                         cGAAATAGTGCGCATGGCGCAGTTTCAGACCTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGCTTGCcagcag                                                                                   >  2:2976728/1‑148 (MQ=255)
                                                          gAAATAGTGCGCATGGCGCAGTTTCAGACCTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGt                                                                                  <  1:2652351/148‑1 (MQ=255)
                                                          gAAATAGTGCGCATGGCGCAGTTTCAGACCTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGt                                                                                  <  2:2855138/148‑1 (MQ=255)
                                                            aaTAGTGCGCATGGCGCAGTTTCAGACCTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGTAc                                                                                >  1:1854723/1‑148 (MQ=255)
                                                              tAGTGCGCATGGCGCAGTTTCAGACCTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGTACCt                                                                              <  2:2392939/148‑1 (MQ=255)
                                                               aGTGCGCATGGCGCAGTTTCAGACCTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGTACCTg                                                                             <  1:1269937/148‑1 (MQ=255)
                                                                  gcgcATGGCGCAGTTTCAGACCTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGTACCTGGGa                                                                          >  2:2131618/1‑148 (MQ=255)
                                                                  gcgcATGGCGCAGTTTCAGACCTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGTACCTGGGa                                                                          >  2:466700/1‑148 (MQ=255)
                                                                      aTGGCGCAGTTTCAGACCTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGTACCTGGGAAGTa                                                                      <  2:2861349/148‑1 (MQ=255)
                                                                          cgcAGTTTCAGACCTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGTACCTGGGAAGTACaaa                                                                  <  2:1434124/148‑1 (MQ=255)
                                                                            cAGTTTCAGACCTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGTACCTGGGAAGTACAAATg                                                                >  1:3077400/1‑148 (MQ=255)
                                                                            cAGTTTCAGACCTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGTACCTGGGAAGTACAAATg                                                                <  2:3248017/148‑1 (MQ=255)
                                                                              gTTTCAGACCTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGTACCTGGGAAGTACAAATGtt                                                              >  2:1914007/1‑148 (MQ=255)
                                                                               tttCAGACCTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGTACCTGGGAAGTACAAATGTTg                                                             >  1:1099581/1‑148 (MQ=255)
                                                                               tttCAGACCTTTATGTAGCAGGCCACCCGCCAGGATATCGGTCAGACGGTGAATGCGGATAGCTCTAAGTTTCAGGCCAACCGGGCCGAGATAAGCGGAAAACAGGCTGGCGATGTATGACATAAGTAACTGGGAAGTACAAATGtag                                                             >  2:294023/1‑146 (MQ=255)
                                                                                   aGACCTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGTACCTGGGAAGTACAAATGTTGGAGt                                                         <  1:1813732/148‑1 (MQ=255)
                                                                                    gACCTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGTACCTGGGAAGTACAAATGTTGGAGtt                                                        >  1:3205684/1‑148 (MQ=255)
                                                                                    gACCTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGTACCTGGGAAGTACAAATGTTGGAGtt                                                        >  1:2116067/1‑148 (MQ=255)
                                                                                    gACCTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGTACCTGGGAAGTACAAATGTTGGAGtt                                                        <  2:206099/148‑1 (MQ=255)
                                                                                      ccTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGTACCTGGGAAGTACAAATGTTGGAGTTCg                                                      <  1:716889/148‑1 (MQ=255)
                                                                                       cTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGTACCTGGGAAGTACAAATGTTGGAGTTCGc                                                     <  2:481069/148‑1 (MQ=255)
                                                                                       cTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGTACCTGGGAAGTACAAATGTTGGAGTTCGc                                                     <  1:1088124/148‑1 (MQ=255)
                                                                                       cTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGTACCTGGGAAGTACAAATGTTGGAGTTCGc                                                     >  1:1419965/1‑148 (MQ=255)
                                                                                        tttttGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGTACCTGGGAAGTACAAATGTTGGAGTTCGCt                                                    >  2:1481381/1‑148 (MQ=255)
                                                                                          tttGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGTACCTGGGAAGTACAAATGTTGGAGTTCGCttt                                                  <  2:1348283/148‑1 (MQ=255)
                                                                                           ttgttgCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGTACCTGGGAAGTACAAATGTTGGAGTTCGCTTtc                                                 <  2:1926409/148‑1 (MQ=255)
                                                                                           ttgttgCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGTACCTGGGAAGTACAAATGTTGGAGTTCGCTTtc                                                 >  1:1595109/1‑148 (MQ=255)
                                                                                           ttgttgCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGTACCTGGGAAGTACAAATGTTGGAGTTCGCTTtc                                                 >  2:3542540/1‑148 (MQ=255)
                                                                                           ttgttgCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGTACCTGGGAAGTACAAATGTTGGAGTTCGCTTtc                                                 <  2:1044948/148‑1 (MQ=255)
                                                                                             gttgCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGTACCTGGGAAGTACAAATGTTGGAGTTCGCTTtctc                                               <  2:2582489/148‑1 (MQ=255)
                                                                                              ttgCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGTACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCa                                              >  2:893233/1‑148 (MQ=255)
                                                                                                    gCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGTACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCAcggcgg                                        <  2:2101623/148‑1 (MQ=255)
                                                                                                     cccGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGTACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGa                                       <  2:826442/148‑1 (MQ=255)
                                                                                                               ggATATCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGTACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTGCTc                             >  2:2656016/1‑148 (MQ=255)
                                                                                                               ggATATCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGTACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTGCTc                             >  2:1783124/1‑148 (MQ=255)
                                                                                                               ggATATCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGTACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTGCTc                             >  2:3401242/1‑148 (MQ=255)
                                                                                                                gATATCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGTACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTGCTcg                            >  1:652617/1‑148 (MQ=255)
                                                                                                                    tCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGTACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTGCTCGCGAg                        >  2:1251262/1‑148 (MQ=255)
                                                                                                                    tCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGTACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTGCTCGCGAg                        <  1:1783124/148‑1 (MQ=255)
                                                                                                                      ggTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGTACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTGCTCGCGAGTc                      <  1:1679740/148‑1 (MQ=255)
                                                                                                                      ggTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGTACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTGCTCGCGAGTc                      <  1:1485343/148‑1 (MQ=255)
                                                                                                                      ggTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGTACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTGCTCGCGAGTc                      <  1:805768/148‑1 (MQ=255)
                                                                                                                        tCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGTACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTGCTCGCGAGTCTg                    <  2:3345668/148‑1 (MQ=255)
                                                                                                                        tCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGTACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTGCTCGCGAGTCTg                    <  2:1753187/148‑1 (MQ=255)
                                                                                                                         cAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGTACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTGCTCGCGAGTCTGc                   <  1:527308/148‑1 (MQ=255)
                                                                                                                         cAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGTACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTGCTCGCGAGTCTGc                   <  1:609452/148‑1 (MQ=255)
                                                                                                                           gACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGTACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTGCTCGCGAGTCTGCAt                 >  2:1837150/1‑148 (MQ=255)
                                                                                                                           gACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGTACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTGCTCGCGAGTCTGCAt                 <  1:1028918/148‑1 (MQ=255)
                                                                                                                           gACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGCTTGCCAGCAGTACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTGCTCGCGAGTCTGCAt                 <  1:2976728/148‑1 (MQ=255)
                                                                                                                               gTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGTACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTGCTCGCGAGTCTGCATCGcc             >  1:220874/1‑148 (MQ=255)
                                                                                                                                  aaTGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGTACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTGCTCGCGAGTCTGCATCGCCATg          <  1:2867195/148‑1 (MQ=255)
                                                                                                                                   aTGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGTACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTGCTCGCGAGTCTGCATCGCCATgc         >  2:765012/1‑148 (MQ=255)
                                                                                                                                         ttAGCTATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGTACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTGCTCGCGAGTCTGCATCGCCATGCGCAgcg   >  2:691338/1‑148 (MQ=255)
                                                                                                                                          tAGCTATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGTACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTGCTCGCGAGTCTGCATCGCCATGCGCAgcgc  >  1:2041651/1‑148 (MQ=255)
                                                                                                                                              |                                                                                                                                               
CCGCTTCGGCACGCGTCAGTACGCCCGCTTTGTCGGCCACTTCCACACACAAGGTGTCGAAATAGTGCGCATGGCGCAGTTTCAGACCTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCGATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGTACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTGCTCGCGAGTCTGCATCGCCATGCGCAGCGC  >  CP009273/3041179‑3041464

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
ATTTCAGCCGCTTCGGCACGCGTCAGTACGCCCGCTTTGTCGGCCACTTCCACACACAAGGTGTCGAAATAGTGCGCATGGCGCAGTTTCAGACCTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCGATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGTACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTGCTCGCGAGTCTGCATCGCCATGCGCAGCGCGGTAT  >  CP009273/3041172‑3041469
                                                                                                                                                     |                                                                                                                                                    
ATTTCAGCCGCTTCGGCACGCGTCAGTACGCCCGCTTTGTCGGCCACTTCCACACACAAGGTGTCGAAATAGTGCGCATGGCGCAGTTTCAGACCTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCT                                                                                                                                                      <  SRR19973067.1657344/150‑1 (MQ=60)
ATTTCAGCCGCTTCGGCACGCGTCAGTACGCCCGCTTTGTCGGCCACTTCCACACACAAGGTGTCGAAATAGTGCGCATGGCGCAGTTTCAGACCTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCT                                                                                                                                                      <  SRR19973067.531995/150‑1 (MQ=60)
ATTTCAGCCGCTTCGGCACGCGTCAGTACGCCCGCTTTGTCGGCCACTTCCACACACAAGGTGTCGAAATAGTGCGCATGGCGCAGTTTCAGACCTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCT                                                                                                                                                      <  SRR19973067.531935/150‑1 (MQ=60)
              GNCACGCGTCAGTACGCCCGCTTTGTCGGCCACTTCCACACACAAGGTGTCGAAATAGTGCGCATGGCGCAGTTTCAGACCTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCC                                                                                                                                        >  SRR19973067.1195524/1‑150 (MQ=60)
               GCACGCGTCAGTACGCCCGCTTTGTCGGCCACTTCCACACACAAGGTGTCGAAATAGTGCGCATGGCGCAGTTTCAGACCTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCA                                                                                                                                       <  SRR19973067.432423/150‑1 (MQ=60)
                  CGCGTCAGTACGCCCGCTTTGTCGGCCACTTCCACACACAAGGTGTCGAAATAGTGCGCATGGCGCAGTTTCAGACCTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACC                                                                                                                                    <  SRR19973067.3140063/150‑1 (MQ=60)
                   actatttcgacaccttgtgtgtggaaACTTCCACACACAAGGTGTCGAAATAGTGCGCATGGCGCAGTTTCAGACCTTTTTGTTGCAGGCCCGCCGCCAGGCTATCGGTCAGACGGTGAATGCGGGTAGCTATACGTTTCAGGCCAACCG                                                                                                                                   >  SRR19973067.509675/27‑150 (MQ=60)
                      TCAGTACGCCCGCTTTGTCGGCCACTTCCACACACAAGGTGTCGAAATAGTGCGCATGGCGCAGTTTCAGACCTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGC                                                                                                                                <  SRR19973067.2596653/150‑1 (MQ=60)
                      TCAGTACGCCCGCTTTGTCGGCCACTTCCACACACAAGGTGTCGAAATAGTGCGCATGGCGCAGTTTCAGACCTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGC                                                                                                                                <  SRR19973067.423289/150‑1 (MQ=60)
                      TCAGTACGCCCGCTTTGTCGGCCACTTCCACACACAAGGTGTCGAAATAGTGCGCATGGCGCAGTTTCAGACCTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGC                                                                                                                                <  SRR19973067.423513/150‑1 (MQ=60)
                       CAGTACGCCCGCTTTGTCGGCCACTTCCACACACAAGGTGTCGAAATAGTGCGCATGGCGCAGTTTCAGACCTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCC                                                                                                                               <  SRR19973067.1465781/150‑1 (MQ=60)
                         GTACGCCCGCTTTGTCGGCCACTTCCACACACAAGGTGTCGAAATAGTGCGCATGGCGCAGTTTCAGACCTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGT                                                                                                                             >  SRR19973067.311882/1‑150 (MQ=60)
                          TACGCCCGCTTTGTCGGCCACTTCCACACACAAGGTGTCGAAATAGTGCGCATGGCGCAGTTTCAGACCTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTG                                                                                                                            >  SRR19973067.214512/1‑150 (MQ=60)
                           ACGCCCGCTTTGTCGGCCACTTCCACACACAAGGTGTCGAAATAGTGCGCATGGCGCAGTTTCAGACCTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGA                                                                                                                           >  SRR19973067.1617793/1‑150 (MQ=60)
                           ACGCCCGCTTTGTCGGCCACTTCCACACACAAGGTGTCGAAATAGTGCGCATGGCGCAGTTTCAGACCTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGA                                                                                                                           >  SRR19973067.1617786/1‑150 (MQ=60)
                            CGCCCGCTTTGTCGGCCACTTCCACACACAAGGTGTCGAAATAGTGCGCATGGCGCAGTTTCAGACCTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGAT                                                                                                                          <  SRR19973067.2362309/150‑1 (MQ=60)
                            CGCCCGCTTTGTCGGCCACTTCCACACACAAGGTGTCGAAATAGTGCGCATGGCGCAGTTTCAGACCTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGAT                                                                                                                          <  SRR19973067.1093090/150‑1 (MQ=60)
                            CGCCCGCTTTGTCGGCCACTTCCACACACAAGGTGTCGAAATAGTGCGCATGGCGCAGTTTCAGACCTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGAT                                                                                                                          <  SRR19973067.2362019/150‑1 (MQ=60)
                             GCCCGCTTTGTCGGCCACTTCCACACACAAGGTGTCGAAATAGTGCGCATGGCGCAGTTTCAGACCTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATA                                                                                                                         >  SRR19973067.3168363/1‑150 (MQ=60)
                             GCCCGCTTTGTCGGCCACTTCCACACACAAGGTGTCGAAATAGTGCGCATGGCGCAGTTTCAGACCTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATA                                                                                                                         >  SRR19973067.3168723/1‑150 (MQ=60)
                             GCCCGCTTTGTCGGCCACTTCCACACACAAGGTGTCGAAATAGTGCGCATGGCGCAGTTTCAGACCTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATA                                                                                                                         >  SRR19973067.3171163/1‑150 (MQ=60)
                             GCCCGCTTTGTCGGCCACTTCCACACACAAGGTGTCGAAATAGTGCGCATGGCGCAGTTTCAGACCTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATA                                                                                                                         >  SRR19973067.3168728/1‑150 (MQ=60)
                                CGCTTTGTCGGCCACTTCCACACACAAGGTGTCGAAATAGTGCGCATGGCGCAGTTTCAGACCTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATAAAC                                                                                                                      <  SRR19973067.3081736/150‑1 (MQ=60)
                                 GCTTTGTCGGCCACTTCCACACACAAGGTGTCGAAATAGTGCGCATGGCGCAGTTTCAGACCTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATAAACG                                                                                                                     >  SRR19973067.1180328/1‑150 (MQ=60)
                                 GCTTTGTCGGCCACTTCCACACACAAGGTGTCGAAATAGTGCGCATGGCGCAGTTTCAGACCTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCGATACGTTTCAGGCCAACCGGGCCGTGATAAACG                                                                                                                     <  SRR19973067.877610/150‑1 (MQ=60)
                                 GCTTTGTCGGCCACTTCCACACACAAGGTGTCGAAATAGTGCGCATGGCGCAGTTTCAGACCTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCGATACGTTTCAGGCCAACCGGGCCGTGATAAACG                                                                                                                     <  SRR19973067.877613/150‑1 (MQ=60)
                                  CTTTGTCGGCCACTTCCACACACAAGGTGTCGAAATAGTGCGCATGGCGAAGTTTCATACCTTTTTGTAGAAGGACCGACGCCAGGATATCGGACAGACGGTGAATGAGGTTCGCTATACGTTTCAGTCCAAAAGGGACGAGATAAACGG                                                                                                                    >  SRR19973067.3441636/1‑150 (MQ=60)
                                        CGGCCACTTCCACACACAAGGTGTCGAAATAGTGCGCATGGCGCAGTTTCAGACCTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACA                                                                                                              >  SRR19973067.1835633/1‑150 (MQ=60)
                                        CGGCCACTTCCACACACAAGGTGTCGAAATAGTGCGCATGGCGCAGTTTCAGACCTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACA                                                                                                              >  SRR19973067.1835655/1‑150 (MQ=60)
                                        CGGCCACTTCCACACACAAGGTGTCGAAATAGTGCGCATGGCGCAGTTTCAGACCTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACA                                                                                                              >  SRR19973067.22136/1‑150 (MQ=60)
                                         GGCCACTTCCACACACAAGGTGTCGAAATAGTGCGCATGGCGCAGTTTCAGACCTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAG                                                                                                             <  SRR19973067.2506774/150‑1 (MQ=60)
                                         GGCCACTTCCACACACAAGGTGTCGAAATAGTGCGCATGGCGCAGTTTCAGACCTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAG                                                                                                             <  SRR19973067.2506740/150‑1 (MQ=60)
                                         GGCCACTTCCACACACAAGGTGTCGAAATAGTGCGCATGGCGCAGTTTCAGACCTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAG                                                                                                             <  SRR19973067.2506757/150‑1 (MQ=60)
                                         GGCCACTTCCACACACAAGGTGTCGAAATAGTGCGCATGGCGCAGTTTCAGACCTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAG                                                                                                             >  SRR19973067.98968/1‑150 (MQ=60)
                                            CACTTCCACACACAAGGTGTCGAAATAGTGCGCATGGCGCAGTTTCAGACCTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCT                                                                                                          >  SRR19973067.1808766/1‑150 (MQ=60)
                                            CACTTCCACACACAAGGTGTCGAAATAGTGCGCATGGCGCAGTTTCAGACCTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCT                                                                                                          >  SRR19973067.2506110/1‑150 (MQ=60)
                                             ACTTCCACACACAAGGTGTCGAAATAGTGCGCATGGCGCAGTTTCAGACCTTTTTGGTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTG                                                                                                         <  SRR19973067.3306157/150‑1 (MQ=60)
                                              CTTCCACACACAAGGTGTCGAAATAGTGCGCATGGCGCAGTTTCAGACCTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGG                                                                                                        >  SRR19973067.3378751/1‑150 (MQ=60)
                                              CTTCCACACACAAGGTGTCGAAATAGTGCGCATGGCGCAGTTTCAGACCTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGG                                                                                                        >  SRR19973067.3378767/1‑150 (MQ=60)
                                                TCCACACACAAGGTGTCGAAATAGTGCGCATGGCGCAGTTTCAGACCTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCG                                                                                                      <  SRR19973067.3405531/150‑1 (MQ=60)
                                                      CACAAGGTGTCGAAATAGTGCGCATGGCGCAGTTTCAGACCTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTT                                                                                                <  SRR19973067.757626/150‑1 (MQ=60)
                                                      CACAAGGTGTCGAAATAGTGCGCATGGCGCAGTTTCAGACCTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTT                                                                                                <  SRR19973067.757630/150‑1 (MQ=60)
                                                      CACAAGGTGTCGAAATAGTGCGCATGGCGCAGTTTCAGACCTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTT                                                                                                <  SRR19973067.936514/150‑1 (MQ=60)
                                                       ACAAGGTGTCGAAATAGTGCGCATGGCGCAGTTTCAGACCTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTG                                                                                               <  SRR19973067.1895628/150‑1 (MQ=60)
                                                         AAGGTGTCGAAATAGTGCGCATGGCGCAGTTTCAGACCTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCC                                                                                             >  SRR19973067.1886023/1‑150 (MQ=60)
                                                          CGGTGTCGAAAGAGTGCGCATGGCGCAGTTTCAGCCCTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCA                                                                                            <  SRR19973067.2526361/150‑1 (MQ=60)
                                                          AGGTGTCGAAATAGTGCGCATGGCGCAGTTTCAGACCTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCA                                                                                            >  SRR19973067.2524249/1‑150 (MQ=60)
                                                                 GAAATAGTGCGCATGGCGCAGTTTCAGACCTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGTAC                                                                                     <  SRR19973067.2669445/150‑1 (MQ=60)
                                                                 GAAATAGTGCGCATGGCGCAGTTTCAGACCTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGTAC                                                                                     >  SRR19973067.1867363/1‑150 (MQ=60)
                                                                      AGTGCGCATGGCGCAGTTTCAGACCTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGTACCTGGG                                                                                <  SRR19973067.1278574/150‑1 (MQ=60)
                                                                      AGTGCGCATGGCGCAGTTTCAGACCTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGTACCTGGG                                                                                <  SRR19973067.1278675/150‑1 (MQ=60)
                                                                       GTGCGCATGGCGCAGTTTCAGACCTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGTACCTGGGA                                                                               <  SRR19973067.347965/150‑1 (MQ=60)
                                                                        TGCGCATGGCGCAGTTTCAGACCTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCGATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGTACCTGGGAA                                                                              <  SRR19973067.2802961/150‑1 (MQ=60)
                                                                             ATGGCGCAGTTTCAGACCTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCGATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGTACCTGGGAAGTACA                                                                         <  SRR19973067.2305921/150‑1 (MQ=60)
                                                                               GGCGCAGTTTCAGACCTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGTACCTGGGAAGTACAAA                                                                       <  SRR19973067.48611/150‑1 (MQ=60)
                                                                                GCGCAGTTTCAGACCTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGTACCTGGGAAGTACAAAT                                                                      <  SRR19973067.2519757/150‑1 (MQ=60)
                                                                                 CGCAGTTTCAGACCTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGTACCTGGGACGTACAAATG                                                                     >  SRR19973067.3096984/1‑150 (MQ=60)
                                                                                 CGCAGTTTCAGACCTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGTACCTGGGAAGTACAAATG                                                                     >  SRR19973067.3096985/1‑150 (MQ=60)
                                                                                 CGCAGTTTCAGACCTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGTACCTGGGAAGTACAAATG                                                                     <  SRR19973067.3358250/150‑1 (MQ=60)
                                                                                   CAGTTTCAGACCTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGTACCTGGGAAGTACAAATGTT                                                                   >  SRR19973067.7883/1‑150 (MQ=60)
                                                                                   CAGTTTCAGACCTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGTACCTGGGAAGTACAAATGTT                                                                   >  SRR19973067.7985/1‑150 (MQ=60)
                                                                                    AGTTTCAGACCTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGTACCTGGGAAGTACAAATGTTG                                                                  >  SRR19973067.1107061/1‑150 (MQ=60)
                                                                                    AGTTTCAGACCTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCGATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGTACCTGGGAAGTACAAATGTTG                                                                  >  SRR19973067.1176063/1‑150 (MQ=60)
                                                                                        TCAGACCTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGTACCTGGGAAGTACAAATGTTGGAGT                                                              <  SRR19973067.545022/150‑1 (MQ=60)
                                                                                        TCAGACCTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGTACCTGGGAAGTACAAATGTTGGAGT                                                              <  SRR19973067.1213282/150‑1 (MQ=60)
                                                                                         CAGACCTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGTACCTGGGAAGTACAAATGTTGGAGTT                                                             >  SRR19973067.3225893/1‑150 (MQ=60)
                                                                                         CAGACCTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGTACCTGGGAAGTACAAATGTTGGAGTT                                                             >  SRR19973067.3223737/1‑150 (MQ=60)
                                                                                         CAGACCTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGTACCTGGGAAGTACAAATGTTGGAGTT                                                             >  SRR19973067.328525/1‑150 (MQ=60)
                                                                                         CAGACCTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGTACCTGGGAAGTACAAATGTTGGAGTT                                                             >  SRR19973067.2130170/1‑150 (MQ=60)
                                                                                          AGACCTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGTACCTGGGAAGTACAAATGTTGGAGTTC                                                            <  SRR19973067.1826007/150‑1 (MQ=60)
                                                                                           GACCTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGTACCTGGGAAGTACAAATGTTGGAGTTCG                                                           <  SRR19973067.1843855/150‑1 (MQ=60)
                                                                                            ACCTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGTACCTGGGAAGTACAAATGTTGGAGTTCGC                                                          >  SRR19973067.1429562/1‑150 (MQ=60)
                                                                                             CCTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGTACCTGGGAAGTACAAATGTTGGAGTTCGCT                                                         <  SRR19973067.721580/150‑1 (MQ=60)
                                                                                             CCTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGTACCTGGGAAGTACAAATGTTGGAGTTCGCT                                                         <  SRR19973067.721859/150‑1 (MQ=60)
                                                                                              CTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGTACCTGGGAAGTACAAATGTTGGAGTTCGCTT                                                        <  SRR19973067.1095587/150‑1 (MQ=60)
                                                                                              CTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGTACCTGGGAAGTACAAATGTTGGAGTTCGCTT                                                        <  SRR19973067.1095557/150‑1 (MQ=60)
                                                                                               TTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGTACCTGGGAAGTACAAATGTTGGAGTTCGCTTT                                                       <  SRR19973067.2204394/150‑1 (MQ=60)
                                                                                                TTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGTACCTGGGAAGTACAAATGTTGGAGTTCGCTTTC                                                      >  SRR19973067.1606049/1‑150 (MQ=60)
                                                                                                     TTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGTACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACG                                                 <  SRR19973067.2095161/150‑1 (MQ=60)
                                                                                                     TTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGTACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACG                                                 <  SRR19973067.575479/150‑1 (MQ=60)
                                                                                                       GCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGTACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGC                                               >  SRR19973067.3314707/1‑150 (MQ=60)
                                                                                                         AGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGCCTGGCGATGTTTGCCAGCAGTACCTGGGAAGTACACATGTTGGAGTTCGCTATCTCACGGCGG                                             >  SRR19973067.1894860/1‑150 (MQ=60)
                                                                                                            CCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGTACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATA                                          >  SRR19973067.87124/1‑150 (MQ=60)
                                                                                                               GCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGTACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGT                                       >  SRR19973067.1963897/1‑150 (MQ=60)
                                                                                                               GCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGTACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGT                                       >  SRR19973067.154213/1‑150 (MQ=60)
                                                                                                                   CCAGGATATCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGTACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTGCT                                   <  SRR19973067.1782625/150‑1 (MQ=60)
                                                                                                                     AGGATATCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGTACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTGCTCG                                 >  SRR19973067.937352/1‑150 (MQ=60)
                                                                                                                     AGGATATCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGTACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTGCTCG                                 >  SRR19973067.938405/1‑150 (MQ=60)
                                                                                                                     AGGATATCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGTACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTGCTCG                                 >  SRR19973067.657047/1‑150 (MQ=60)
                                                                                                                     AGGATATCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGTACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTGCTCG                                 >  SRR19973067.41495/1‑150 (MQ=60)
                                                                                                                     AGGATATCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGTACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTGCTCG                                 >  SRR19973067.3032366/1‑150 (MQ=60)
                                                                                                                     AGGATATCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGTACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTGCTCG                                 >  SRR19973067.3032348/1‑150 (MQ=60)
                                                                                                                         TATCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGTACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTGCTCGCGAG                             >  SRR19973067.3170451/1‑150 (MQ=60)
                                                                                                                           TCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGTACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTGCTCGCGAGTC                           <  SRR19973067.3422895/150‑1 (MQ=60)
                                                                                                                           TCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGTACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTGCTCGCGAGTC                           <  SRR19973067.1795173/150‑1 (MQ=60)
                                                                                                                           TCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGTACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTGCTCGCGAGTC                           <  SRR19973067.1793944/150‑1 (MQ=60)
                                                                                                                            CGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGTACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTGCTCGCGAGTCT                          <  SRR19973067.150609/150‑1 (MQ=60)
                                                                                                                             GGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGTACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTGCTCGCGAGTCTG                         <  SRR19973067.811312/150‑1 (MQ=60)
                                                                                                                             GGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGTACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTGCTCGCGAGTCTG                         <  SRR19973067.1495186/150‑1 (MQ=60)
                                                                                                                             GGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGTACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTGCTCGCGAGTCTG                         <  SRR19973067.1691316/150‑1 (MQ=60)
                                                                                                                             GGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGTACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTGCTCGCGAGTCTG                         <  SRR19973067.1691180/150‑1 (MQ=60)
                                                                                                                             GGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGTACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTGCTCGCGAGTCTG                         <  SRR19973067.2605628/150‑1 (MQ=60)
                                                                                                                                CAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGTACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTGCTCGCGAGTCTGCAT                      <  SRR19973067.613388/150‑1 (MQ=60)
                                                                                                                                CAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGTACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTGCTCGCGAGTCTGCAT                      <  SRR19973067.530854/150‑1 (MQ=60)
                                                                                                                                  GACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGTACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTGCTCGCGAGTCTGCATCG                    >  SRR19973067.2154210/1‑150 (MQ=60)
                                                                                                                                  GACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGTACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTGCTCGCGAGTCTGCATCG                    <  SRR19973067.1035999/150‑1 (MQ=60)
                                                                                                                                  GACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCACCAGTACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTGCTCGCGAGTCTGCATCG                    <  SRR19973067.2608853/150‑1 (MQ=60)
                                                                                                                                  GACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGCTTGCCAGCAGTACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTGCTCGCGAGTCTGCATCG                    <  SRR19973067.2995509/150‑1 (MQ=60)
                                                                                                                                    CGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGTACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTGCTCGCGAGTCTGCATCGCC                  >  SRR19973067.222489/1‑150 (MQ=60)
                                                                                                                                    CGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGTACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTGCTCGCGAGTCTGCATCGCC                  >  SRR19973067.640059/1‑150 (MQ=60)
                                                                                                                                      GTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGTACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTGCTCGCGAGTCTGCATCGCCAT                <  SRR19973067.2912422/150‑1 (MQ=60)
                                                                                                                                         AATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGTACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTGCTCGCGAGTCTGCATCGCCATGCG             <  SRR19973067.2885558/150‑1 (MQ=60)
                                                                                                                                               GTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGTACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTGCTCGCGAGTCTGCATCGCCATGCGCAGCGC       >  SRR19973067.2055307/1‑150 (MQ=60)
                                                                                                                                                    CTATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGTACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTGCTCGCGAGTCTGCATCGCCATGCGCAGCGCGGTAT  >  SRR19973067.1388789/1‑150 (MQ=60)
                                                                                                                                                    CTATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGTACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTGCTCGCGAGTCTGCATCGCCATGCGCAGCGCGGTAT  <  SRR19973067.3284326/150‑1 (MQ=60)
                                                                                                                                                    CTATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGTACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTGCTCGCGAGTCTGCATCGCCATGCGCAGCGCGGTAT  <  SRR19973067.1071037/150‑1 (MQ=60)
                                                                                                                                                     |                                                                                                                                                    
ATTTCAGCCGCTTCGGCACGCGTCAGTACGCCCGCTTTGTCGGCCACTTCCACACACAAGGTGTCGAAATAGTGCGCATGGCGCAGTTTCAGACCTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCGATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGTACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTGCTCGCGAGTCTGCATCGCCATGCGCAGCGCGGTAT  >  CP009273/3041172‑3041469

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑