Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F368 I0 R1 1472 214.4 7116750 95.9% 6824963 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,549,090 C→A 9.0% R883S (CGC→AGC)  malT → mal regulon transcriptional activator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,549,0900CA 9.0% 748.5 / 31.7 211R883S (CGC→AGC) malTmal regulon transcriptional activator
Reads supporting (aligned to +/- strand):  ref base C (88/104);  new base A (7/12);  total (95/116)
Fisher's exact test for biased strand distribution p-value = 4.81e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01

Only 100 of 217 total aligned reads displayed.

AGCCCGCTGACGCAACGTGAATGGCAGGTACTGGGGCTGATCTACTCTGGTTACAGCAATGAGCAAATTGCCGGAGAACTGGAAGTCGCGGCAACCACCATCAAAACGCATATCCGCAATCTGTATCAGAAACTCGGCGTGGCCCATCGCCAGGATGCGGTACAACACGCCCAGCAATTGCTGAAGATGATGGGGTACGGCGTGTAAGTTTAGCCGGATAACGCGCCAGATCCGGCTTACATCTCTGCATCATTCAATGCTCACCCGCGTTACGCCATCTGTTTCTATCAAACTA  >  CP009273/3548943‑3549237
                                                                                                                                                   |                                                                                                                                                   
aGCCCGCTGACGCAACGTGAATGGCAGGTACTGGGGCTGATCTACTCTGGTTACAGCAATGAGCAAATTGCCGGAGAACTGGAAGTCGCGGCAACCACCATCAAAACGCATATCCGCAATCTGTATCAGAAACTCGGCGTGGCCCATc                                                                                                                                                     >  1:1356205/1‑148 (MQ=255)
aGCCCGCTGACGCAACGTGAATGGCAGGTACTGGGGCTGATCTACTCTGGTTACAGCAATGAGCAAATTGCCGGAGAACTGGAAGTCGCGGCAACCACCATCAAAACGCATATCCGCAATCTGTATCAGAAACTCGGCGTGGCCCATc                                                                                                                                                     >  1:3126492/1‑148 (MQ=255)
 gCCCGCTGACGCAACGTGAATGGCAGGTACTGGGGCTGATCTACTCTGGTTACAGCAATGAGCAAATTGCCGGAGAACTGGAAGTCGCGGCAACCACCATCAAAACGCATATCCGCAATCTGTATCAGAAACTCGGCGTGGCCCATCg                                                                                                                                                    >  2:3525577/1‑148 (MQ=255)
   ccGCTGACGCAACGTGAATGGCAGGTACTGGGGCTGATCTACTCTGGTTACAGCAATGAGCAAATTGCCGGAGAACTGGAAGTCGCGGCAACCACCATCAAAACGCATATCCGCAATCTGTATCAGAAACTCGGCGTGGCCCATCGcc                                                                                                                                                  <  1:2306167/148‑1 (MQ=255)
     gCTGACGCAACGTGAATGGCAGGTACTGGGGCTGATCTACTCTGGTTACAGCAATGAGCAAATTGCCGGAGAACTGGAAGTCGCGGCAACCACCATCAAAACGCATATCCGCAATCTGTATCAGAAACTCGGCGTGGCCCATCGCCAg                                                                                                                                                >  2:697117/1‑148 (MQ=255)
       tGACGCAACGTGAATGGCAGGTACTGGGGCTGATCTACTCTGGTTACAGCAATGAGCAAATTGCCGGAGAACTGGAAGTCGCGGCAACCACCATCAAAACGCATATCCGCAATCTGTATCAGAAACTCGGCGTGGCCCATCGCCAGGa                                                                                                                                              <  1:575801/148‑1 (MQ=255)
        gACGCAACGTGAATGGCAGGTACTGGGGCTGATCTACTCTGGTTACAGCAATGAGCAAATTGCCGGAGAACTGGAAGTCGCGGCAACCACCATCAAAACGCATATCCGCAATCTGTATCAGAAACTCGGCGTGGCCCATCGCCAGGAt                                                                                                                                             >  2:2156413/1‑148 (MQ=255)
            cAACGTGAATGGCAGGTACTGGGGCTGATCTACTCTGGTTACAGCAATGAGCAAATTGCCGGAGAACTGGAAGTCGCGGCAACCACCATCAAAACGCATATCCGCAATCTGTATCAGAAACTCGGCGTGGCCCATCGCCAGGATGCgg                                                                                                                                         >  2:2210897/1‑148 (MQ=255)
            cAACGTGAATGGCAGGTACTGGGGCTGATCTACTCTGGTTACAGCAATGAGCAAATTGCCGGAGAACTGGAAGTCGCGGCAACCACCATCAAAACGCATATCCGCAATCTGTATCAGAAACTCGGCGTGGCCCATCGCCAGGATGCgg                                                                                                                                         <  1:2120753/148‑1 (MQ=255)
             aaCGTGAATGGCAGGTACTGGGGCTGATCTACTCTGGTTACAGCAATGAGCAAATTGCCGGAGAACTGGAAGTCGCGGCAACCACCATCAAAACGCATATCCGCAATCTGTATCAGAAACTCGGTGTGGCCCATCGCCAGGATGCGGt                                                                                                                                        <  1:3286726/148‑1 (MQ=255)
             aaCGTGAATGGCAGGTACTGGGGCTGATCTACTCTGGTTACAGCAATGAGCAAATTGCCGGAGAACTGGAAGTCGCGGCAACCACCATCAAAACGCATATCCGCAATCTGTATCAGAAACTCGGCGTGGCCCATCGCCAGGATGCGGt                                                                                                                                        <  1:2446292/148‑1 (MQ=255)
              aCGTGAATGGCAGGTACTGGGGCTGATCTACTCTGGTTACAGCAATGAGCAAATTGCCGGAGAACTGGAAGTCGCGGCAACCACCATCAAAACGCATATCCGCAATCTGTATCAGAAACTCGGCGTGGCCCATCGCCAGGATGCGGTa                                                                                                                                       >  2:1004786/1‑148 (MQ=255)
                  gAATGGCAGGTACTGGGGCTGATCTACTCTGGTTACAGCAATGAGCAAATTGCCGGAGAACTGGAAGTCGCGGCAACCACCATCAAAACGCATATCCGCAATCTGTATCAGAAACTCGGCGTGGCCCATCGCCAGGATGCGGTacaac                                                                                                                                   >  2:2735022/1‑148 (MQ=255)
                   aaTGGCAGGTACTGGGGCTGATCTACTCTGGTTACAGCAATGAGCAAATTGCCGGAGAACTGGAAGTCGCGGCAACCACCATCAAAACGCATATCCGCAATCTGTATCAGAAACTCGGCGTGGCCCATCGCCAGGATGCGGTacaaca                                                                                                                                  >  2:2585138/1‑148 (MQ=255)
                     tGGCAGGTACTGGGGCTGATCTACTCTGGTTACAGCAATGAGCAAATTGCCGGAGAACTGGAAGTCGCGGCAACCACCATCAAAACGCATATCCGCAATCTGTATCAGAAACTCGGCGTGGCCCATCGCCAGGATGCGGTACAACACg                                                                                                                                >  2:2494372/1‑148 (MQ=255)
                      ggCAGGTACTGGGGCTGATCTACTCTGGTTACAGCAATGAGCAAATTGCCGGAGAACTGGAAGTCGCGGCAACCACCATCAAAACGCATATCCGCAATCTGTATCAGAAACTCGGCGTGGCCCATCGCCAGGATGCGGTACAACACGc                                                                                                                               <  1:1314517/148‑1 (MQ=255)
                                 gggCTGATCTACTCTGGTTACAGCAATGAGCAAATTGCCGGAGAACTGGAAGTCGCGGCAACCACCATCAAAACGCATATCCGCAATCTGTATCAGAAACTCGGCGTGGCCCATCGCCAGGATGCGGTACAACACGCCCAGCAATTGc                                                                                                                    <  2:574969/148‑1 (MQ=255)
                                 gggCTGATCTACTCTGGTTACAGCAATGAGCAAATTGCCGGAGAACTGGAAGTCGCGGCAACCACCATCAAAACGCATATCCGCAATCTGTATCAGAAACTCGGCGTGGCCCATAGCCAGGATGCGGTACAACACGCCCAGCAATTGc                                                                                                                    >  2:3069243/1‑148 (MQ=255)
                                  ggCTGATCTACTCTGGTTACAGCAATGAGCAAATTGCCGGAGAACTGGAAGTCGCGGCAACCACCATCAAAACGCATATCCGCAATCTGTATCAGAAACTCGGCGTGGCCCATCGCCAGGATGCGGTACAACACGCCCAGCAATTGCt                                                                                                                   <  1:2676779/148‑1 (MQ=255)
                                  ggCTGATCTACTCTGGTTACAGCAATGAGCAAATTGCCGGAGAACTGGAAGTCGCGGCAACCACCATCAAAACGCATATCCGCAATCTGTATCAGAAACTCGGCGTGGCCCATCGCCAGGATGCGGTACAACACGCCCAGCAATTGCt                                                                                                                   >  1:1350854/1‑148 (MQ=255)
                                   gCTGATCTACTCTGGTTACAGCAATGAGCAAATTGCCGGAGAACTGGAAGTCGCGGCAACCACCATCAAAACGCATATCCGCAATCTGTATCAGAAACTCGGCGTGGCCCATCGCCAGGATGCGGTACAACACGCCCAGCAATTGCTg                                                                                                                  <  1:510442/148‑1 (MQ=255)
                                      gATCTACTCTGGTTACAGCAATGAGCAAATTGCCGGAGAACTGGAAGTCGCGGCAACCACCATCAAAACGCATATCCGCAATCTGTATCAGAAACTCGGCGTGGCCCATCGCCAGGATGCGGTACAACACGCCCAGCAATTGCTGAAg                                                                                                               <  1:235708/148‑1 (MQ=255)
                                       aTCTACTCTGGTTACAGCAATGAGCAAATTGCCGGAGAACTGGAAGTCGCGGCAACCACCATCAAAACGCATATCCGCAATCTGTATCAGAAACTCGGCGTGGCCCATCGCCAGGATGCGGTACAACACGCCCAGCAATTGCTGAAga                                                                                                              >  2:1357204/1‑148 (MQ=255)
                                        tCTACTCTGGTTACAGCAATGAGCAAATTGCCGGAGAACTGGAAGTCGCGGCAACCACCATCAAAACGCATATCCGCAATCTGTATCAGAAACTCGGCGTGGCCCATCGCCAGGATGCGGTACAACACGCCCAGCAATTGCTGAAgat                                                                                                             <  2:291688/148‑1 (MQ=255)
                                         cTACTCTGGTTACAGCAATGAGCAAATTGCCGGAGAACTGGAAGTCGCGGCAACCACCATCAAAACGCATATCCGCAATCTGTATCAGAAACTCGGCGTGGCCCATCGCCAGGATGCGGTACAACACGCCCAGCAATTGCTGAAgatg                                                                                                            >  2:2259676/1‑148 (MQ=255)
                                          tACTCTGGTTACAGCAATGAGCAAATTGCCGGAGAACTGGAAGTCGCGGCAACCACCATCAAAACGCATATCCGCAATCTGTATCAGAAACTCGGCGTGGCCCATAGCCAGGATGCGGTACAACACGCCCAGCAATTGCTGAAgatga                                                                                                           <  2:2976113/148‑1 (MQ=255)
                                           aCTCTGGTTACAGCAATGAGCAAATTGCCGGAGAACTGGAAGTCGCGGCAACCACCATCAAAACGCATATCCGCAATCTGTATCAGAAACTCGGCGTGGCCCATCGCCAGGATGCGGTACAACACGCCCAGCAATTGCTGAAgatgat                                                                                                          >  1:2708146/1‑148 (MQ=255)
                                            ctctGGTTACAGCAATGAGCAAATTGCCGGAGAACTGGAAGTCGCGGCAACCACCATCAAAACGCATATCCGCAATCTGTATCAGAAACTCGGCGTGGCCCATCGCCAGGATGCGGTACAACACGCCCAGCAATTGCTGAAGatgatg                                                                                                         <  1:2576415/148‑1 (MQ=255)
                                             tctGGTTACAGCAATGAGCAAATTGCCGGAGAACTGGAAGTCGCGGCAACCACCATCAAAACGCATATCCGCAATCTGTATCAGAAACTCGGCGTGGCCCATCGCCAGGATGCGGTACAACACGCCCAGCAATTGCTGAAGATGATgg                                                                                                        >  2:3050669/1‑148 (MQ=255)
                                              ctGGTTACAGCAATGAGCAAATTGCCGGAGAACTGGAAGTCGCGGCAACCACCATCAAAACGCATATCCGCAATCTGTATCAGAAACTCGGCGTGGCCCATCGCCAGGATGCGGTACAACACGCCCAGCAATTGCTGAAGATGATggg                                                                                                       <  1:2161841/148‑1 (MQ=255)
                                               tGGTTACAGCAATGAGCAAATTGCCGGAGAACTGGAAGTCGCGGCAACCACCATCAAAACGCATATCCGCAATCTGTATCAGAAACTCGGCGTGGCCCATCGCCAGGATGCGGTACAACACGCCCAGCAATTGCTGAAGATGATgggg                                                                                                      <  2:1270864/148‑1 (MQ=255)
                                                ggTTACAGCAATGAGCAAATTGCCGGAGAACTGGAAGTCGCGGCAACCACCATCAAAACGCATATCCGCAATCTGTATCAGAAACTCGGCGTGGCCCATCGCCAGGATGCGGTACAACACGCCCAGCAATTGCTGAAGATGATGGGGt                                                                                                     <  1:95252/148‑1 (MQ=255)
                                                ggTTACAGCAATGAGCAAATTGCCGGAGAACTGGAAGTCGCGGCAACCACCATCAAAACGCATATCCGCAATCTGTATCAGAAACTCGGCGTGGCCCATCGCCAGGATGCGGTACAACACGCCCAGCAATTGCTGAAGATGATGGGGt                                                                                                     <  2:760236/148‑1 (MQ=255)
                                                  ttACAGCAATGAGCAAATTGCCGGAGAACTGGAAGTCGCGGCAACCACCATCAAAACGCATATCCGCAATCTGTATCAGAAACTCGGCGTGGCCCATCGCCAGGATGCGGTACAACACGCCCAGCCATTGCTGAAGATGATGGGGTAc                                                                                                   >  2:1334537/1‑148 (MQ=255)
                                                  ttACAGCAATGAGCAAATTGCCGGAGAACTGGAAGTCGCGGCAACCACCATCAAAACGCATATCCGCAATCTGTATCAGAAACTCGGCGTGGCCCATAGCCAGGATGCGGTACAACACGCCCAGCAATTGCTGAAGATGATGGGGTAc                                                                                                   >  1:2218023/1‑148 (MQ=255)
                                                    aCAGCAATGAGCAAATTGCCGGAGAACTGGAAGTCGCGGCAACCACCATCAAAACGCATATCCGCAATCTGTATCAGAAACTCGGCGTGGCCCATCGCCAGGATGCGGTACAACACGCCCAGCAATTGCTGAAGATGATGGGGTACgg                                                                                                 >  1:1278426/1‑148 (MQ=255)
                                                        cAATGAGCAAATTGCCGGAGAACTGGAAGTCGCGGCAACCACCATCAAAACGCATATCCGCAATCTGTATCAGAAACTCGGCGTGGCCCATCGCCAGGATGCGGTACAACACGCCCAGCAATTGCTGAAGATGATGGGGTACGGCgtg                                                                                             <  2:3452842/148‑1 (MQ=255)
                                                          aTGAGCAAATTGCCGGAGAACTGGAAGTCGCGGCAACCACCATCAAAACGCATATCCGCAATCTGTATCAGAAACTCGGCGTGGCCCATCGCCAGGATGCGGTACAACACGCCCAGCAATTGCTGAAGATGATGGGGTACGGCGTGTa                                                                                           <  1:2741054/148‑1 (MQ=255)
                                                          aTGAGCAAATTGCCGGAGAACTGGAAGTCGCGGCAACCACCATCAAAACGCATATCCGCAATCTGTATCAGAAACTCGGCGTGGCCCATCGCCAGGATGCGGTACAACACGCCCAGCAATTGCTGAAGATGATGGGGTACGGCGTGTa                                                                                           >  2:1821333/1‑148 (MQ=255)
                                                             aGCAAATTGCCGGAGAACTGGAAGTCGCGGCAACCACCATCAAAACGCATATCCGCAATCTGTATCAGAAACTCGGCGTGGCCCATCGCCAGGATGCGGTACAACACGCCCAGCAATTGCTGAAGATGATGGGGTACGGCGTGTAAGt                                                                                        >  2:2090295/1‑148 (MQ=255)
                                                             aGCAAATTGCCGGAGAACTGGAAGTCGCGGCAACCACCATCAAAACGCATATCCGCAATCTGTATCAGAAACTCGGCGTGGCCCATAGCCAGGATGCGGTACAACACGCCCAGCAATTGCTGAAGATGATGGGGTACGGCGTGTAAGt                                                                                        <  2:2316503/148‑1 (MQ=255)
                                                              gCAAATTGCCGGAGAACTGGAAGTCGCGGCAACCACCATCAAAACGCATATCCGCAATCTGTATCAGAAACTCGGCGTGGCCCATCGCCAGGATGCGGTACAACACGCCCAGCAATTGCTGAAGATGATGGGGTACGGCGTGTAAGtt                                                                                       <  1:1089516/148‑1 (MQ=255)
                                                               cAAATTGCCGGAGAACTGGAAGTCGCGGCAACCACCATCAAAACGCATATCCGCAATCTGTATCAGAAACTCGGCGTGGCCCATCGCCAGGATGCGGTACAACACGCCCAGCAATTGCTGAAGATGATGGGGTACGGCGTGTAAGttt                                                                                      <  2:1221326/148‑1 (MQ=255)
                                                                  aTTGCCGGAGAACTGGAAGTCGCGGCAACCACCATCAAAACGCATATCCGCAATCTGTATCAGAAACTCGGCGTGGCCCATCGCCAGGATGCGGTACAACACGCCCAGCAATTGCTGAAGATGATGGGGTACGGCGTGTAAGTTTAGc                                                                                   >  2:1541199/1‑148 (MQ=255)
                                                                     gCCGGAGAACTGGAAGTCGCGGCAACCACCATCAAAACGCATATCCGCAATCTGTATCAGAAACTCGGCGTGGCCCATCGCCAGGATGCGGTACAACACGCCCAGCAATTGCTGAAGATGATGGGGTACGGCGTGTAAGTTTAGCCgg                                                                                >  1:356859/1‑148 (MQ=255)
                                                                      ccGGAGAACTGGAAGTCGCGGCAACCACCATCAAAACGCATATCCGCAATCTGTATCAGAAACTCGGCGTGGCCCATCGCCAGGATGCGGTACAACACGCCCAGCAATTGCTGAAGATGATGGGGTACGGCGTGTAAGTTTAGCCGGa                                                                               <  1:2123175/148‑1 (MQ=255)
                                                                       cGGAGAACTGGAAGTCGCGGCAACCACCATCAAAACGCATATCCGCAATCTGTATCAGAAACTCGGCGTGGCCCATCGCCAGGATGCGGTACAACACGCCCAGCAATTGCTGAAGATGATGGGGTACGGCGTGTAAGTTTAGCCGGAt                                                                              <  2:1963019/148‑1 (MQ=255)
                                                                        ggAGAACTGGAAGTCGCGGCAACCACCATCAACACGCATATCCGCAATCTGTATCAGAAACTCGGCGTGGCCCATCGCCAGGATGCGGTACAACACGCCCAGCAATTGCTGAAGATGATGGGGTACGGCGTGTAAGTTTAGCCGGATa                                                                             <  1:1710946/148‑1 (MQ=255)
                                                                        ggAGAACTGGAAGTCGCGGCAACCACCATCAAAACGCATATCCGCAATCTGTATCAGAAACTCGGCGTGGCCCATCGCCAGGATGCGGTACAACACGCCCAGCAATTGCTGAAGATGATGGGGTACGGCGTGTAAGTTTAGCCGGATa                                                                             <  1:1004786/148‑1 (MQ=255)
                                                                             aCTGGAAGTCGCGGCAACCACCATCAAAACGCATATCCGCAATCTGTATCAGAAACTCGGCGTGGCCCATAGCCAGGATGCGGTACAACACGCCCAGCAATTGCTGAAGATGATGGGGTACGGCGTGTAAGTTTAGCCGGATAAcgcg                                                                        >  2:1916571/1‑148 (MQ=255)
                                                                             aCTGGAAGTCGCGGCAACCACCATCAAAACGCATATCCGCAATCTGTATCAGAAACTCGGCGTGGCCCATAGCCAGGATGCGGTACAACACGCCCAGCAATTGCTGAAGATGATGGGGTACGGCGTGTAAGTTTAGCCGGATAAcgcg                                                                        >  2:27976/1‑148 (MQ=255)
                                                                                  aaGTCGCGGCAACCACCATCAAAACGCATATCCGCAATCTGTATCAGAAACTCGGCGTGGCCCATCGCCAGGATGCGGTACAACACGCCCAGCAATTGCTGAAGATGATGGGGTACGGCGTGTAAGTTTAGCCGGATAACGCGCCAGa                                                                   >  2:1018015/1‑148 (MQ=255)
                                                                                    gTCGCGGCAACCACCATCAAAACGCATATCCGCAATCTGTATCAGAAACTCGGCGTGGCCCATCGCCAGGATGCGGTACAACACGCCCAGCAATTGCTGAAGATGATGGGGTACGGCGTGTAAGTTTAGCCGGATAACGCGCCAGATc                                                                 <  1:996119/148‑1 (MQ=255)
                                                                                    gTCGCGGCAACCACCATCAAAACGCATATCCGCAATCTGTATCAGAAACTCGGCGTGGCCCATCGCCAGGATGCGGTACAACACGCCCAGCAATTGCTGAAGATGATGGGGTACGGCGTGTAAGTTTAGCCGGATAACGCGCCAGATc                                                                 >  1:1487475/1‑148 (MQ=255)
                                                                                    gTCGCGGCAACCACCATCAAAACGCATATCCGCAATCTGTATCAGAAACTCGGCGTGGCCCATCGCCAGGATGCGGTACAACACGCCCAGCAATTGCTGAAGATGATGGGGTACGGCGTGTAAGTTTAGCCGGATAACGCGCCAGATc                                                                 <  1:1583112/148‑1 (MQ=255)
                                                                                    gTCGCGGCAACCACCATCAAAACGCATATCCGCAATCTGTATCAGAAACTCGGCGTGGCCCATCGCCAGGATGCGGTACAACACGCCCAGCAATTGCTGAAGATGATGGGGTACGGCGTGTAAGTTTAGCCGGATAACGCGCCAGATc                                                                 <  1:2774370/148‑1 (MQ=255)
                                                                                    gTCGCGGCAACCACCATCAAAACGCATATCCGCAATCTGTATCAGAAACTCGGCGTGGCCCATCGCCAGGATGCGGTACAACACGCCCAGCAATTGCTGAAGATGATGGGGTACGGCGTGTAAGTTTAGCCGGATAACGCGCCAGATc                                                                 <  1:3344511/148‑1 (MQ=255)
                                                                                        cgGCAACCACCATCAACACGCATATCCGCAATCTGTATCAGAAACTCGGCGTGGCCCATCGCCAGGATGCGGTACAACACGCCCAGCAATTGCTGAAGATGATGGGGTACGGCGTGTAAGTTTAGCCGGATAACGCGCCAGATCCGGc                                                             <  1:760569/148‑1 (MQ=255)
                                                                                         ggCAACCACCATCAAAACGCATATCCGCAATCTGTATCAGAAACTCGGCGTGGCCCATCGCCAGGATGCGGTACAACACGCCCAGCAATTGCTGAAGATGATGGGGTACGGCGTGTAAGTTTAGCCGGATAACGCGCCAGATCCGGCt                                                            <  1:2585138/148‑1 (MQ=255)
                                                                                                   aTCAAAACGCATATCCGCAATCTGTATCAGAAACTCGGCGTGGCCCATCGCCAGGATGCGGTACAACACGCCCAGCAATTGCTGAAGATGATGGGGTACGGCGTGTAAGTTTAGCCGGATAACGCGCCAGATCCGGCTTACATCTCTg                                                  <  1:3044176/148‑1 (MQ=255)
                                                                                                    tCAAAACGCATATCCGCAATCTGTATCAGAAACTCGGCGTGGCCCATCGCCAGGATGCGGTACAACACGCCCAGCAATTGCTGAAGATGATGGGGTACGGCGTGTAAGTTTAGCCGGATAACGCGCCAGATCCGGCTTACATCTCTGc                                                 >  2:1121169/1‑148 (MQ=255)
                                                                                                    tCAAAACGCATATCCGCAATCTGTATCAGAAACTCGGCGTGGCCCATCGCCAGGATGCGGTACAACACGCCCAGCAATTGCTGAAGATGATGGGGTACGGCGTGTAAGTTTAGCCGGATAACGCGCCAGATCCGGCTTACATCTCTGc                                                 >  2:286362/1‑148 (MQ=255)
                                                                                                        aaCGCATATCCGCAATCTGTATCAGAAACTCGGCGTGGCCCATAGCCAGGATGCGGTACAACACGCCCAGCAATTGCTGAAGATGATGGGGTACGGCGTGTAAGTTTAGCCGGATAACGCGCCAGATCCGGCTTACATCTCTGcatca                                             <  1:3415269/148‑1 (MQ=255)
                                                                                                         aCGCATATCCGCAATCTGTATCAGAAACTCGGCGTGGCCCATCGCCAGGATGCGGTACAACACGCCCAGCAATTGCTGAAGATGATGGGGTACGGCGTGTAAGTTTAGCCGGATAACGCGCCAGATCCGGCTTACATCTCTGcatcat                                            >  2:686761/1‑148 (MQ=255)
                                                                                                          cGCATATCCGCAATCTGTATCAGAAACTCGGCGTGGCCCATCGCCAGGATGCGGTACAACACGCCCAGCAATTGCTGAAGATGATGGGGTACGGCGTGTAAGTTTAGCCGGATAACGCGCCAGATCCGGCTTACATCTCTGCATCAtt                                           >  2:2171461/1‑148 (MQ=255)
                                                                                                          cGCATATCCGCAATCTGTATCAGAAACTCGGCGTGGCCCATCGCCAGGATGCGGTACAACACGCCCAGCAATTGCTGAAGATGATGGGGTACGGCGTGTAAGTTTAGCCGGATAACGCGCCAGATCCGGCTTACATCTCTGCATCAtt                                           >  2:418191/1‑148 (MQ=255)
                                                                                                          cGCATATCCGCAATCTGTATCAGAAACTCGGCGTGGCCCATCGCCAGGATGCGGTACAACACGCCCAGCAATTGCTGAAGATGATGGGGTACGGCGTGTAAGTTTAGCCGGATAACGCGCCAGATCCGGCTTACATCTCTGCATCAtt                                           >  2:1355280/1‑148 (MQ=255)
                                                                                                               atCCGCAATCTGTATCAGAAACTCGGCGTGGCCCATCGCCAGGATGCGGTACAACACGCCCAGCAATTGCTGAAGATGATGGGGTACGGCGTGTAAGTTTAGCCGGATAACGCGCCAGATCCGGCTTACATCTCTGCATCATTCAATg                                      >  2:3081100/1‑148 (MQ=255)
                                                                                                               atCCGCAATCTGTATCAGAAACTCGGCGTGGCCCATCGCCAGGATGCGGTACAACACGCCCAGCAATTGCTGAAGATGATGGGGTACGGCGTGTAAGTTTAGCCGGATAACGCGCCAGATCCGGCTTACATCTCTGCATCATTCAATg                                      >  1:1149778/1‑148 (MQ=255)
                                                                                                                 ccGCAATCTGTATCAGAAACTCGGCGTGGCCCATCGCCAGGATGCGGTACAACACGCCCAGCAATTGCTGAAGATGATGGGGTACGGCGTGTAAGTTTAGCCGGATAACGCGCCAGATCCGGCTTACATCTCTGCATCATTCAATGCt                                    <  1:198606/148‑1 (MQ=255)
                                                                                                                   gCAATCTGTATCAGAAACTCGGCGTGGCCCATCGCCAGGATGCGGTACAACACGCCCAGCAATTGCTGAAGATGATGGGGTACGGCGTGTAAGTTTAGCCGGATAACGCGCCAGATCCGGCTTACATCTCTGCATCATTCAATGCTCa                                  >  2:342747/1‑148 (MQ=255)
                                                                                                                      aTCTGTATCAGAAACTCGGCGTGGCCCATCGCCAGGATGCGGTACAACACGCCCAGCAATTGCTGAAGATGATGGGGTACGGCGTGTAAGTTTAGCCGGATAACGCGCCAGATCCGGCTTACATCTCTGCATCATTCAATGCTCAccc                               >  2:2392630/1‑148 (MQ=255)
                                                                                                                      aTCTGTATCAGAAACTCGGCGTGGCCCATCGCCAGGATGCGGTACAACACGCCCAGCAATTGCTGAAGATGATGGGGTACGGCGTGTAAGTTTAGCCGGATAACGCGCCAGATCCGGCTTACATCTCTGCATCATTCAATGCTCAccc                               >  1:3080046/1‑148 (MQ=255)
                                                                                                                       tCTGTATCAGAAACTCGGCGTGGCCCATCGCCAGGATGCGGTACAACACGCCCAGCAATTGCTGAAGATGATGGGGTACGGCGTGTAAGTTTAGCCGGATAACGCGCCAGATCCGGCTTACATCTCTGCATCATTCAATGCTCACCcg                              >  2:679405/1‑148 (MQ=255)
                                                                                                                             tCAGAAACTCGGCGTGGCCCATCGCCAGGATGCGGTACAACACGCCCAGCAATTGCTGAAGATGATGGGGTACGGCGTGTAAGTTTAGCCGGATAACGCGCCAGATCCGGCTTACATCTCTGCATCATTCAATGCTCACCCGCGTTAc                        <  1:1927657/148‑1 (MQ=255)
                                                                                                                               aGAAACTCGGCGTGGCCCATCGCCAGGATGCGGTACAACACGCCCAGCAATTGCTGAAGATGATGGGGTACGGCGTGTAAGTTTAGCCGGATAACGCGCCAGATCCGGCTTACATCTCTGCATCATTCAATGCTCACCCGCGTTACGc                      <  1:2156413/148‑1 (MQ=255)
                                                                                                                                 aaaCTCGGCGTGGCCCATCGCCAGGATGCGGTACAACACGCCCAGCAATTGCTGAAGATGATGGGGTACGGCGTGTAAGTTTAGCCGGATAACGCGCCAGATCCGGCTTACATCTCTGCATCATTCAATGCTCACCCGCGTTACGCCa                    <  1:2084464/148‑1 (MQ=255)
                                                                                                                                  aaCTCGGCGTGGCCCATCGCCAGGATGCGGTACAACACGCCCAGCAATTGCTGAAGATGATGGGGTACGGCGTGTAAGTTTAGCCGGATAACGCGCCAGATCCGGCTTACATCTCTGCATCATTCAATGCTCACCCGCGTTACGCCAt                   <  1:1781322/148‑1 (MQ=255)
                                                                                                                                  aaCTCGGCGTGGCCCATCGCCAGGATGCGGTACAACACGCACAGCAATTGCTGAAGATGATGGGGTACGGCGTGTAAGTTTAGCCGGATAACGCGCCAGATCCGGCTTACATCTCTGCATCATTCAATGCTCACCCGCGTTACGCCAt                   <  2:734630/148‑1 (MQ=255)
                                                                                                                                  aaCTCGGCGTGGCCCATAGCCAGGATGCGGTACAACACGCCCAGCAATTGCTGAAGATGATGGGGTACGGCGTGTAAGTTTAGCCGGATAACGCGCCAGATCCGGCTTACATCTCTGCATCATTCAATGCTCACCCGCGTTACGCCAt                   <  1:3499337/148‑1 (MQ=255)
                                                                                                                                   aCTCGGCGTGGCCCATCGCCAGGATGCGGTACAACACGCCCAGCAATTGCTGAAGATGATGGGGTACGGCGTGTAAGTTTAGCCGGATAACGCGCCAGATCCGGCTTACATCTCTGCATCATTCAATGCTCACCCGCGTTACGCCATc                  >  1:18727/1‑148 (MQ=255)
                                                                                                                                    cTCGGCGTGGCCCATCGCCAGGATGCGGTACAACACGCCCAGCAATTGCTGAAGATGATGGGGTACGGCGTGTAAGTTTAGCCGGATAACGCGCCAGATCCGGCTTACATCTCTGCATCATTCAATGCTCACCCGCGTTACGCCATCt                 <  1:1166587/148‑1 (MQ=255)
                                                                                                                                    cTCGGCGTGGCCCATCGCCAGGATGCGGTACAACACGCCCAGCAATTGCTGAAGATGATGGGGTACGGCGTGTAAGTTTAGCCGGATAACGCGCCAGATCCGGCTTACATCTCTGCATCATTCAATGCTCACCCGCGTTACGCCATCt                 >  1:2804217/1‑148 (MQ=255)
                                                                                                                                     tCGGCGTGGCCCATCGCCAGGATGCGGTACAACACGCCCAGCAATTGCTGAAGATGATGGGGTACGGCGTGTAAGTTTAGCCGGATAACGCGCCAGATCCGGCTTACATCTCTGCATCATTCAATGCTCACCCGCGTTACGCCATCTg                <  2:2587841/148‑1 (MQ=255)
                                                                                                                                     tCGGCGTGGCCCATCGCCAGGATGCGGTACAACACGCCCAGCAATTGCTGAAGATGATGGGGTACGGCGTGTAAGTTTAGCCGGATAACGCGCCAGATCCGGCTTACATCTCTGCATCATTCAATGCTCACCCGCGTTACGCCATCTg                >  2:2174165/1‑148 (MQ=255)
                                                                                                                                     tCGGCGTGGCCCATCGCCAGGATGCGGTACAACACGCCCAGCAATTGCTGAAGATGATGGGGTACGGCGTGTAAGTTTAGCCGGATAACGCGCCAGATCCGGCTTACATCTCTGCATCATTCAATGCTCACCCGCGTTACGCCATCTg                >  1:371916/1‑148 (MQ=255)
                                                                                                                                     tCGGCGTGGCCCATCGCCAGGATGCGGTACAACACGCCCAGCAATTGCTGAAGATGATGGGGTACGGCGTGTAAGTTTAGCCGGATAACGCGCCAGATCCGGCTTACATCTCTGCATCATTCAATGCTCACCCGCGTTACGCCATCTg                >  2:2574617/1‑148 (MQ=255)
                                                                                                                                      cGGCGTGGCCCATCGCCAGGATGCGGTACAACACGCCCAGCAATTGCTGAAGATGATGGGGTACGGCGTGTAAGTTTAGCCGGATAACGCGCCAGATCCGGCTTACATCTCTGCATCATTCAATGCTCACCCGCGTTACGCCATCTGt               <  2:3280161/148‑1 (MQ=255)
                                                                                                                                      cGGCGTGGCCCATCGCCAGGATGCGGTACAACACGCCCAGCAATTGCTGAAGATGATGGGGTACGGCGTGTAAGTTTAGCCGGATAACGCGCCAGATCCGGCTTACATCTCTGCATCATTCAATGCTCACCCGCGTTACGCCATCTGt               >  1:735644/1‑148 (MQ=255)
                                                                                                                                      cGGCGTGGCCCATCGCCAGGATGCGGTACAACACGCCCAGCAATTGCTGAAGATGATGGGGTACGGCGTGTAAGTTTAGCCGGATAACGCGCCAGATCCGGCTTACATCTCTGCATCATTCAATGCTCACCCGCGTTACGCCATCTGt               <  1:697245/148‑1 (MQ=255)
                                                                                                                                      cGGCGTGGCCCATAGCCAGGATGCGGTACAACACGCCCAGCAATTGCTGAAGATGATGGGGTACGGCGTGTAAGTTTAGCCGGATAACGCGCCAGATCCGGCTTACATCTCTGCATCATTCAATGCTCACCCGCGTTACGCCATCTGt               <  1:693593/148‑1 (MQ=255)
                                                                                                                                        gCGTGGCCCATCGCCAGGATGCGGTACAACACGCCCAGCAATTGCTGAAGATGATGGGGTACGGCGTGTAAGTTTAGCCGGATAACGCGCCAGATCCGGCTTACATCTCTGCATCATTCAATGCTCACCCGCGTTACGCCATCTGttt             <  2:3295377/148‑1 (MQ=255)
                                                                                                                                          gTGGCCCATAGCCAGGATGCGGTACAACACGCCCAGCAATTGCTGAAGATGATGGGGTACGGCGTGTAAGTTTAGCCGGATAACGCGCCAGATCCGGCTTACATCTCTGCATCATTCAATGCTCACCCGCGTTACGCCATCTGTTTCt           <  2:469699/148‑1 (MQ=255)
                                                                                                                                          gTGGCCCATAGCCAGGATGCGGTACAACACGCCCAGCAATTGCGGAAGATGATGGGGTACGGCGTGTAAGTTTAGCCGGATAACGCGCCAGATCCGGCTTACATCTCTGCATCATTCAATGCTCACCCGCGTTACGCCATCTGTTTCt           <  2:623714/148‑1 (MQ=255)
                                                                                                                                               ccATCGCCAGGATGCGGTACAACACGCCCAGCAATTGCTGAAGATGATGGGGTACGGCGTGTAAGTTTAGCCGGATAACGCGCCAGATCCGGCTTACATCTCTGCATCATTCAATGCTCACCCGCGTTACGCCATCTGTTTCTATCaa      <  2:913988/148‑1 (MQ=255)
                                                                                                                                               ccAGCGCCAGGATGCGGTACAACACGCCCAGCAATTGCTGAAGATGATGGGGTACGGCGTGTAAGTTTAGCCGGATAACGCGCCAGATCCGGCTTACATCTCTGCATCATTCAATGCTCACCCGCGTTACGCCATCTGTTTCTATCaa      <  2:913975/148‑1 (MQ=255)
                                                                                                                                                cATCGCCAGGATGCGGTACAACACGCCCAGCAATTGCTGAAGATGATGGGGTACGGCGTGTAAGTTTAGCCGGATAACGCGCCAGATCCGGCTTACATCTCTGCATCATTCAATGCTCACCCGCGTTACGCCATCTGTTTCTATCaaa     <  1:1541199/148‑1 (MQ=255)
                                                                                                                                                cATCGCCAGGATGCGGTACAACACGCCCAGCAATTGCTGAAGATGATGGGGTACGGCGTGTAAGTTTAGCCGGATAACGCGCCAGATCCGGCTTACATCTCTGCATCATTCAATGCTCACCCGCGTTACGCCATCTGTTTCTATCaaa     <  1:1946413/148‑1 (MQ=255)
                                                                                                                                                 aTCGCCAGGATGCGGTACAACACGCCCAGCAATTGCTGAAGATGATGGGGTACGGCGTGTAAGTTTAGCCGGATAACGCGCCAGATCCGGCTTACATCTCTGCATCATTCAATGCTCACCCGCGTTACGCCATCTGTTTCTATCAAAc    >  2:535303/1‑148 (MQ=255)
                                                                                                                                                   cGCCAGGATGCGGTACAACACGCCCAGCAATTGCTGAAGATGATGGGGTACGGCGTGTAAGTTTAGCCGGATAACGCGCCAGATCCGGCTTACATCTCTGCATCATTCAATGCTCACCCGCGTTACGCCATCTGTTTCTATCAAACTa  <  1:770647/148‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
AGCCCGCTGACGCAACGTGAATGGCAGGTACTGGGGCTGATCTACTCTGGTTACAGCAATGAGCAAATTGCCGGAGAACTGGAAGTCGCGGCAACCACCATCAAAACGCATATCCGCAATCTGTATCAGAAACTCGGCGTGGCCCATCGCCAGGATGCGGTACAACACGCCCAGCAATTGCTGAAGATGATGGGGTACGGCGTGTAAGTTTAGCCGGATAACGCGCCAGATCCGGCTTACATCTCTGCATCATTCAATGCTCACCCGCGTTACGCCATCTGTTTCTATCAAACTA  >  CP009273/3548943‑3549237

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A