Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F394 I0 R1 1342 215.0 7134094 96.2% 6862998 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 435,862 A→T 100% T232T (ACT→ACA)  ispA ← geranyltranstransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273435,8620AT100.0% 452.5 / ‑6.7 166T232T (ACT→ACA) ispAgeranyltranstransferase
Reads supporting (aligned to +/- strand):  ref base A (2/3);  major base T (68/87);  minor base G (6/0);  total (76/90)
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 166 total aligned reads displayed.

TTCAGCCAGTTGTTTCAGCGACTGACGGGCATCGTCGATCAGATCCCGGGCTTTCTTCCGGGCTTGCTCAAGACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCAGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCGGCGCTTAATGCACCAAGGCGAACGGCGGCGCGAATCAA  >  CP009273/435721‑436005
                                                                                                                                             |                                                                                                                                               
ttCAGCCAGTTGTTTCAGCGACTGACGGGCATCGTCGATCAGATCCCGGGCTTTCTTCCGGGCTTGCTCAAGACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTACCAACGTTGCGGTAtct                                                                                                                                           >  2:1060768/1‑148 (MQ=255)
ttCAGCCAGTTGTTTCAGCGACTGACGGGCATCGTCGATCAGATCCCGGGCTTTCTTCCGGGCTTGCTCAAGACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTACCAAAGTTGCTGTAtct                                                                                                                                           >  2:1219048/1‑148 (MQ=255)
 tCAGCCAGTTGTTTCAGCGACTGACGGGCATCGTCGATCAGATCCCGGGCTTTCTTCCGGGCTTGCTCAAGACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCTGTAtctc                                                                                                                                          <  1:1385419/148‑1 (MQ=255)
 tCAGCCAGTTGTTTCAGCGACTGACGGGCATCGTCGATCAGATCCCGGGCTTTCTTCCGGGCTTGCTCAAGACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCTGTAtctc                                                                                                                                          <  1:1386953/148‑1 (MQ=255)
 tCAGCCAGTTGTTTCAGCGACTGACGGGCATCGTCGATCAGATCCCGGGCTTTCTTCCGGGCTTGCTCAAGACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCTGTAtctc                                                                                                                                          <  1:1830881/148‑1 (MQ=255)
  aaGCCAGTTGTTTCAGCGACTGACGGGCATCGTCGATCAGATCCCGGGCTTTCTTCCGGGCTTGCTCAAGACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCTGTATCTcc                                                                                                                                         <  1:3279013/147‑1 (MQ=255)
     ccAGTTGTTTCAGCGACTGACGGGCATCGTCGATCAGATCCCGGGCTTTCTTCCGGGCTTGCTCAAGACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCTGTATCTCccac                                                                                                                                      <  1:2314190/148‑1 (MQ=255)
     ccAGTTGTTTCAGCGACTGACGGGCATCGTCGATCAGATCCCGGGCTTTCTTCCGGGCTTGCTCAAGACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCTGTATCTCccac                                                                                                                                      >  2:3160422/1‑148 (MQ=255)
       aGTTGTTTCAGCGACTGACGGGCATCGTCGATCAGATCCCGGGCTTTCTTCCGGGCTTGCTCAAGACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCCACGTTGCGGTATATCccacca                                                                                                                                    >  1:2804971/1‑148 (MQ=255)
       aGTTGTTTCAGCGACTGACGGGCATCGTCGATCAGATCCCGGGCTTTCTTCCGGGCTTGCTCAAGACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCTGTATCTCccacca                                                                                                                                    <  2:1057517/148‑1 (MQ=255)
        gttgttTCAGCGACTGACGGGCATCGTCGATCAGATCCCGGGCTTTCTTCCGGGCTTGCTCAAGACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGGTGTTTATACcaccac                                                                                                                                   >  1:51060/1‑148 (MQ=255)
        gttgttTCAGCGACTGACGGGCATCGTCGATCAGATCCCGGGCTTTCTTCCGGGCTTGCTCAAGACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCTGTATCTCCccccac                                                                                                                                   >  1:2046701/1‑148 (MQ=255)
         ttgttTCAGCGACTGACGGGCATCGTCGATCAGATCCCGGGCTTTCTTCCGGGCTTGCTCAAGACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCTGTATCTCCCACcaca                                                                                                                                  <  1:3122783/148‑1 (MQ=255)
         ttgttTCAGCGACTGACGGGCATCGTCGATCAGATCCCGGGCTTTCTTCCGGGCTTGCTCAAGACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCTGTATCTCCCACcaca                                                                                                                                  <  1:324932/148‑1 (MQ=255)
         ttgttTCAGCGACTGACGGGCATCGTCGATCAGATCCCGGGCTTTCTTCCGGGCTTGCTCAAGACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCTGTATCTACCACcaca                                                                                                                                  >  2:59841/1‑148 (MQ=255)
          tgttTCAGCGACTGACGGGCATCGTCGATCAGATCCCGGGCTTTCTTCCGGGCTTGCTCAAGACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCTGTATCTCCCACCACAt                                                                                                                                 >  2:2915182/1‑148 (MQ=255)
          tgttTCAGCGACTGACGGGCATCGTCGATCAGATCCCGGGCTTTCTTCCGGGCTTGCTCAAGACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCTGTATCTCCCACCACAt                                                                                                                                 >  2:1645224/1‑148 (MQ=255)
           gttTCAGCGACTGACGGGCATCGTCGATCAGATCCCGGGCTTTCTTCCGGGCTTGCTCAAGACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCTGTATCTCCCACCACATc                                                                                                                                <  2:1381384/148‑1 (MQ=255)
           gttTCAGCGACTGACGGGCATCGTCGATCAGATCCCGGGCTTTCTTCCGGGCTTGCTCAAGACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCTGTATATCCCACCACATc                                                                                                                                >  2:2518448/1‑148 (MQ=255)
           gttTCAGCGACTGACGGGCATCGTCGATCAGATCCCGGGCTTTCTTCCGGGCTTGCTCAAGACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGGTGCTGTATCTCCCACCACATc                                                                                                                                >  2:738091/1‑148 (MQ=255)
            tttCAGCGACTGACGGGCATCGTCGATCAGATCCCGGGCTTTCTTCCGGGCTTGCTCAAGACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCTGTATCTCCCACCACATcc                                                                                                                               <  2:2867530/148‑1 (MQ=255)
                aGCGACTGACGGGCATCGTCGATCAGATCCCGGGCTTTCTTCCGGGCTTGCTCAAGACCCAGAAGTGCAGGGTAGGTACTTTTATCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCTGTATCTCCCACCACATCCAGGa                                                                                                                           <  2:3341161/148‑1 (MQ=255)
                aGCGACTGACGGGCATCGTCGATCAGATCCCGGGCTTTCTTCCGGGCTTGCTCAAGACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCTGTATCTCCCACCACATCCAGGa                                                                                                                           <  2:1811781/148‑1 (MQ=255)
                  cGACTGACGGGCATCGTCGATCAGATCCCGGGCTTTCTTCCGGGCTTGCTCAAGACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCTGTATCTCCCACCACATCCACGATg                                                                                                                         >  1:1114440/1‑148 (MQ=255)
                      tGACGGGCATCGTCGATCAGATCCCGGGCTTTCTTCCGGGCTTGCTCAAGACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCTGTATCTCCCACCACATCCAGGATGTCAt                                                                                                                     <  2:2609640/148‑1 (MQ=255)
                      tGACGGGCATCGTCGATCAGATCCCGGGCTTTCTTCCGGGCTTGCTCAAGACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCTGTATCTCCCACCACATCCAGGATGTCAt                                                                                                                     <  2:2609758/148‑1 (MQ=255)
                       gACGGGCATCGTCGATCAGATCCCGGGCTTTCTTCCGGGCTTGCTCAAGACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCTGTATCTCCCACCACATCCAGGATGTCATc                                                                                                                    >  2:412064/1‑148 (MQ=255)
                        aCGGGCATCGTCGATCAGATCCCGGGCTTTCTTCCGGGCTTGCTCAAGACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCTGTATCTCCCACCACATCCAGGATGTCATcc                                                                                                                   <  2:2114087/148‑1 (MQ=255)
                        aCGGGCATCGTCGATCAGATCCCGGGCTTTCTTCCGGGCTTGCTCAAGACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCTGTATCTCCCACCACATCCAGGATGTCATcc                                                                                                                   >  2:660374/1‑148 (MQ=255)
                             cATCGTCGATCAGATCCCGGGCTTTCTTCCGGGCTTGCTCAAGACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCTGTATCTCCCACCACATCCAGGATGTCATCCTGAAc                                                                                                              <  1:3540583/148‑1 (MQ=255)
                               tcgtcgATCAGATCCCGGGCTTTCTTCCGGGCTTGCTCAAGACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTACCAAACTTTCTGTATATACCACCACAAACAGGAATTCATACGGaaaca                                                                                                            >  1:401874/1‑145 (MQ=255)
                                cgtcgATCAGATCCCGGGCTTTCTTCCGGGCTTGCTCAAGACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCTGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTg                                                                                                           <  2:2935076/148‑1 (MQ=255)
                                   cgATCAGATCCCGGGCTTTCTTCCGGGCTTGCTCAAGACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCTGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGaa                                                                                                        >  2:2517141/1‑148 (MQ=255)
                                    gATCAGATCCCGGGCTTTCTTCCGGGCTTGCTCAAGACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCTGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGaag                                                                                                       <  1:1033705/148‑1 (MQ=255)
                                     aTCAGATCCCGGGCTTTCTTCCGGGCTTGCTCAAGACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTTCCAACCTTGCTGTATATCCCACAACAAACACGATCTCAACCTGAAACAGGaagg                                                                                                      >  1:1819873/1‑148 (MQ=255)
                                          aTCCCGGGCTTTCTTCCGGGCTTGCTCAAGACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCAGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGaaggcaagg                                                                                                 >  2:3114873/1‑148 (MQ=255)
                                           tCCCGGGCTTTCTTCCGGGCTTGCTCAAGACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCTGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGaaggcaaggc                                                                                                <  2:3508936/148‑1 (MQ=255)
                                            cccGGGCTTTCTTCCGGGCTTGCTCAAGACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCTGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGcc                                                                                               >  2:1741357/1‑148 (MQ=255)
                                             ccGGGCTTTCTTCCGGGCTTGCTCAAGACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCTGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCg                                                                                              <  1:2260150/148‑1 (MQ=255)
                                             ccGGGCTTTCTTCCGGGCTTGCTCAAGACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCTGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCg                                                                                              <  1:405170/148‑1 (MQ=255)
                                                ggCTTTCTTCCGGGCTTGCTCAAGACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCTGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATg                                                                                           >  2:463355/1‑148 (MQ=255)
                                                  cTTTCTTCCGGGCTTGCTCAAGACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCCACGTTGCTGTATCTCCCACCCCAACCACGATGTCATCCTGAAACTGGAAGGCAAGGCCGATGct                                                                                         >  1:3388410/1‑148 (MQ=255)
                                                  cTTTCTTCCGGGCTTGCTCAAGACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCTGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGct                                                                                         <  1:3070021/148‑1 (MQ=255)
                                                    ttcttcCGGGCTTGCTCAAGACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCTGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGctct                                                                                       <  2:110422/148‑1 (MQ=255)
                                                         ccGGGCTTGCTCAAGACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCTGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCa                                                                                  <  2:2039372/148‑1 (MQ=255)
                                                               ttGCTCAAGACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCTGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTg                                                                            <  2:1063287/148‑1 (MQ=255)
                                                                  cTCAAGACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCTGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCg                                                                         <  2:636546/148‑1 (MQ=255)
                                                                   tCAAGACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCTGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGa                                                                        <  2:2046701/148‑1 (MQ=255)
                                                                    cAAGACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCTGTATCTCCCACCACAACCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAg                                                                       >  2:1347465/1‑148 (MQ=255)
                                                                    cAAGACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCTGTATCTACCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCAGCATACTTGTCGAg                                                                       >  2:2999849/1‑148 (MQ=255)
                                                                     aaGACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCAGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGt                                                                      <  2:138849/148‑1 (MQ=255)
                                                                           cAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCTGTATCTCCCACCACATACAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCGGc                                                                >  1:2643464/1‑148 (MQ=255)
                                                                             gAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCTGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCGGCag                                                              >  2:2598224/1‑148 (MQ=255)
                                                                             gAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACCTTGCTGTAACTACCACCACAAACAGGATGTCATCCTGAACCTGGGAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCGGCag                                                              >  1:1298810/1‑148 (MQ=255)
                                                                             gAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTACCAACGTTGCTGTATCTCCCACCACAACCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTTTCGAGTACCGGCag                                                              >  2:2412213/1‑148 (MQ=255)
                                                                              aaGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCTGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCGGCaga                                                             >  2:572747/1‑148 (MQ=255)
                                                                              aaGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCTGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCGGCaga                                                             >  2:104646/1‑148 (MQ=255)
                                                                              aaGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGGTTCTGTATATACCACCACATCCAGGATGTCATACTGAAACTGGAAGGCAACGCCGATGCTCTCTGCATAATTTTCTAGTAACGGCaga                                                             >  1:2362668/1‑148 (MQ=255)
                                                                                gTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCTGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCGGCAGAGc                                                           <  2:522502/148‑1 (MQ=255)
                                                                                 tGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTTCGGTATCTCCCACCACATACAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGATTACCGGCAGAGCa                                                          >  2:322026/1‑148 (MQ=255)
                                                                                 tGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCTGTATCTCCCACCACATCCAGGATGTGCTCCTGAAACAGGAAGGCAACGCCGATGCTCTCTGCATACTTGTCGAGTACCGGCAGAGCa                                                          >  1:515446/1‑148 (MQ=255)
                                                                                         aggtaCTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCACCGTTGCTGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCGGCAGAGCACGACGTcc                                                  <  1:1627953/148‑1 (MQ=255)
                                                                                         aggtaCTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCTGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCGGCAGAGCACGACGTcc                                                  <  1:3364152/148‑1 (MQ=255)
                                                                                         aggtaCTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCTGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCGGCAGAGCACGACGTcc                                                  <  1:1627881/148‑1 (MQ=255)
                                                                                         aggtaCTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCTGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCGGCAGAGCACGACGTcc                                                  <  1:2146206/148‑1 (MQ=255)
                                                                                          ggtaCTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCTGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCGGCAGAGCACGACGTCCt                                                 >  2:1474484/1‑148 (MQ=255)
                                                                                           gtaCTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCTGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCGGCAGAGCACGACGTCCtt                                                <  2:2298754/148‑1 (MQ=255)
                                                                                           gtaCTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCTGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCGGCAGAGCACGACGTCCtt                                                <  2:3373531/148‑1 (MQ=255)
                                                                                             aCTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCTGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCGGCAGACCACGACGGCCGTTa                                              >  2:653299/1‑148 (MQ=255)
                                                                                             aCTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGGTGCTGTATCTCCCACCACAACCACGATGTCATCCTGAACCAGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCGGCAGAGCACGACGTCCGTTa                                              >  1:3077255/1‑148 (MQ=255)
                                                                                               ttttACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGGTGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCGGCAGAGCACGACGTCCTTTAtc                                            >  1:1449713/1‑148 (MQ=255)
                                                                                                tttACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCTGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCGGCAGAGCACGACGTCCTTTAtct                                           <  2:3542062/148‑1 (MQ=255)
                                                                                                 ttACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCTGTATCTCCCACCACAACCCGGATGTCATACAGAACCTGGAAGGCAAGGCCGATGCTCTCAGCATAATTGTCGAGTACCCGCAGAGCACCACGTCCATTAtctc                                          >  1:1942814/1‑148 (MQ=255)
                                                                                                     cAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCTGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCGgc                                      <  2:372393/148‑1 (MQ=255)
                                                                                                     cAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCTGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCGgc                                      <  2:2168841/148‑1 (MQ=255)
                                                                                                        ggtGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCTGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCGGCGCt                                   <  2:2220781/146‑1 (MQ=255)
                                                                                                         ttGCTGGTCGGCACCCTGGCGTTTTCCCCACGTTGGGGTATCTCCCACCACAACCAGGCTGTCATCCGGAACCTGGAAGGCCAGGCCGCTGCTCTCTGCATACATGTGGAGTACCGGCCGAGCACGACGTCCGGTATCTCCGgcgcgt                                  >  1:1013906/1‑146 (MQ=255)
                                                                                                         ttGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCTGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGGAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCGGCGCtt                                  >  2:1233389/1‑148 (MQ=255)
                                                                                                         ttGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCTGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATATCCGGCGCtt                                  >  1:2045733/1‑148 (MQ=255)
                                                                                                          tGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCTGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCGGCGCTTa                                 <  1:463355/148‑1 (MQ=255)
                                                                                                          tGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCTGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCGGCGCTTa                                 <  2:2276887/148‑1 (MQ=255)
                                                                                                          agCTGGACGGCACCCCGGCCTTTTCCCAACGTTGCTGTATCGCCCACCACATCCACGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCGGCGCTTa                                 <  2:1252085/147‑1 (MQ=255)
                                                                                                                   gCACCCTGGCGTTTTCCCAACGTTGCTGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCGGCGCTTAATGCACCaa                        <  2:2759458/148‑1 (MQ=255)
                                                                                                                   gCACCCTGGCGTTTTCCCAACGTTGCTGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCGGCGCTTAATGCACCaa                        <  2:606275/148‑1 (MQ=255)
                                                                                                                    cACCCTGGCGTTTTCCCAACGTTGCTGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCGGCGCTTAATGCACCAAg                       <  2:401874/148‑1 (MQ=255)
                                                                                                                     aCCCTGGCGTTTTCCCAACGTTGCTGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGTTGCTCTCTGCATACTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCGGCGCTTAATGCACCAAgg                      <  2:2044235/148‑1 (MQ=255)
                                                                                                                     aCCCTGGCGTTTTCCCAACGTTGCTGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCGGCGCTTAATGCACCAAgg                      <  2:315574/148‑1 (MQ=255)
                                                                                                                     aCCCTGGCGTTTTCCCAACGTTGCTGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCGGCGCTTAATGCACCAAgg                      <  1:104646/148‑1 (MQ=255)
                                                                                                                      cccTGGCGTTTTCCCAACGTTGCTGTATCTCCCACCACATCCAGGATGTCATCCTGAAACTGGAAGGCAAGGCCGATGCTCTCTGCATAATTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCGGCGCTTAATGCACCAAGGc                     >  1:584241/1‑148 (MQ=255)
                                                                                                                         tGGCGTTTTCCCAACGTTGCTGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCGGCGCTTAATGCACCAAGGCGaa                  >  1:1498990/1‑148 (MQ=255)
                                                                                                                          ggCGTTTTCCCAACGTTGCTGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCGGCGCTTAATGCACCAAGGCGAAc                 >  1:3048405/1‑148 (MQ=255)
                                                                                                                           gCGTTTTCCCAACGTTGCTGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCGGCGCTTAATGCACCAAGGCGAAcg                <  1:1571965/148‑1 (MQ=255)
                                                                                                                              ttttCCCAACGTTGCTGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCGGCGCTTAATGCACCAAGGCGAAcggcg             >  2:855276/1‑148 (MQ=255)
                                                                                                                              ttttCCCAACGTTGCTGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCGGCGCTTAATGCACCAAGGCGAAcggcg             >  2:817903/1‑148 (MQ=255)
                                                                                                                              ttttCCCAACGTTGCTGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCGGCGCTTAATGCACCAAGGCGAAcggcg             >  2:2996217/1‑148 (MQ=255)
                                                                                                                               tttCCCAACGTTGCTGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCGGCGCTTAATGCACCAAGGCGAAcggcgg            <  1:1474484/148‑1 (MQ=255)
                                                                                                                                 tCCCAACGTTGCTGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCGGCGCTTAATGCACCAAGGCGAACGgcggcg          <  1:3164252/148‑1 (MQ=255)
                                                                                                                                      aCGTTGCTGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCGGCGCTTAATGCACCAAGGCGAACGGCGGCGCGAAt     <  2:1819873/148‑1 (MQ=255)
                                                                                                                                       cGTTGCTGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCGGCGCTTAATGCACCAAGGCGAACGGCGGCGCGAATc    <  1:1741357/148‑1 (MQ=255)
                                                                                                                                         ttGCTGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCGGCGCTTAATGCACCAAGGCGAACGGCGGCGCGAATCaa  >  2:176939/1‑148 (MQ=255)
                                                                                                                                             |                                                                                                                                               
TTCAGCCAGTTGTTTCAGCGACTGACGGGCATCGTCGATCAGATCCCGGGCTTTCTTCCGGGCTTGCTCAAGACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCAGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCGGCGCTTAATGCACCAAGGCGAACGGCGGCGCGAATCAA  >  CP009273/435721‑436005

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GTGACTGTTCAGCCAGTTGTTTCAGCGACTGACGGGCATCGTCGATCAGATCCCGGGCTTTCTTCCGGGCTTGCTCAAGACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCAGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCGGCGCTTAATGCACCAAGGCGAACGGCGGCGCGAATCAA  >  CP009273/435714‑436005
                                                                                                                                                    |                                                                                                                                               
GTGACTGTTCAGCCAGTTGTTTCAGCGACTGACGGGCATCGTCGATCAGATCCCGGGCTTTCTTCCGGGCTTGCTCAAGACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCTG                                                                                                                                                <  SRR19973066.291332/150‑1 (MQ=60)
 TGACTGTTCAGCCAGTTGTTTCAGCGACTGACGGGCATCGTCGATCAGATCCCGGGCTTTCTTCCGGGCTTGCTCAAGACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCTGT                                                                                                                                               <  SRR19973066.1354485/150‑1 (MQ=60)
 TGACTGTTCAGCCAGTTGTTTCAGCGACTGACGGGCATCGTCGATCAGATCCCGGGCTTTCTTCCGGGCTTGCTCAAGACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCTGT                                                                                                                                               <  SRR19973066.1354167/150‑1 (MQ=60)
  GACTGTTCAGCCAGTTGTTTCAGCGACTGACGGGCATCGTCGATCAGATCCCGGGCTTTCTTCCGGGCTTGCTCAAGACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCTGTA                                                                                                                                              <  SRR19973066.529141/150‑1 (MQ=60)
  GACTGTTCAGCCAGTTGTTTCAGCGACTGACGGGCATCGTCGATCAGATCCCGGGCTTTCTTCCGGGCTTGCTCAAGACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCTGTA                                                                                                                                              <  SRR19973066.2347011/150‑1 (MQ=60)
        TCAGCCAGTTGTTTCAGCGACTGACGGGCATCGTCGATCAGATCCCGGGCTTTCTTCCGGGCTTGCTCAAGACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCTGTATCTCCC                                                                                                                                        <  SRR19973066.1395428/150‑1 (MQ=60)
        TCAGCCAGTTGTTTCAGCGACTGACGGGCATCGTCGATCAGATCCCGGGCTTTCTTCCGGGCTTGCTCAAGACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCTGTATCTCCC                                                                                                                                        <  SRR19973066.1843954/150‑1 (MQ=60)
        TCAGCCAGTTGTTTCAGCGACTGACGGGCATCGTCGATCAGATCCCGGGCTTTCTTCCGGGCTTGCTCAAGACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCTGTATCTCCC                                                                                                                                        <  SRR19973066.1847383/150‑1 (MQ=60)
        TCAGCCAGTTGTTTCAGCGACTGACGGGCATCGTCGATCAGATCCCGGGCTTTCTTCCGGGCTTGCTCAAGACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCTGTATCTCCC                                                                                                                                        <  SRR19973066.1396972/150‑1 (MQ=60)
         CAGCCAGTTGTTTCAGCGACTGACGGGCATCGTCGATCAGATCCCGGGCTTTCTTCCGGGCTTGCTCAAGACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCTGTATCTCCCA                                                                                                                                       <  SRR19973066.3300535/150‑1 (MQ=60)
         AAGCCAGTTGTTTCAGCGACTGACGGGCATCGTCGATCAGATCCCGGGCTTTCTTCCGGGCTTGCTCAAGACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCTGTATCTCCCA                                                                                                                                       <  SRR19973066.3300548/150‑1 (MQ=60)
            CCAGTTGTTTCAGCGACTGACGGGCATCGTCGATCAGATCCCGGGCTTTCTTCCGGGCTTGCTCAAGACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCCACGTTGCGGTATATCCCACCA                                                                                                                                    >  SRR19973066.2823848/1‑150 (MQ=60)
            CCAGTTGTTTCAGCGACTGACGGGCATCGTCGATCAGATCCCGGGCTTTCTTCCGGGCTTGCTCAAGACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCTGTATCTCCCACCA                                                                                                                                    <  SRR19973066.2330025/150‑1 (MQ=60)
             CAGTTGTTTCAGCGACTGACGGGCATCGTCGATCAGATCCCGGGCTTTCTTCCGGGCTTGCTCAAGACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCTGTATCTCCCCCCAC                                                                                                                                   >  SRR19973066.2060934/1‑150 (MQ=60)
               GTTGTTTCAGCGACTGACGGGCATCGTCGATCAGATCCCGGGCTTTCTTCCGGGCTTGCTCAAGACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCTGTATCTCCCACCACAT                                                                                                                                 <  SRR19973066.1606802/150‑1 (MQ=60)
                TTGTTTCAGCGACTGACGGGCATCGTCGATCAGATCCCGGGCTTTCTTCCGGGCTTGCTCAAGACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCTGTATCTCCCACCACATC                                                                                                                                <  SRR19973066.327396/150‑1 (MQ=60)
                TTGTTTCAGCGACTGACGGGCATCGTCGATCAGATCCCGGGCTTTCTTCCGGGCTTGCTCAAGACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCTGTATCTCCCACCACATC                                                                                                                                <  SRR19973066.3143639/150‑1 (MQ=60)
                 TGTTTCAGCGACTGACGGGCATCGTCGATCAGATCCCGGGCTTTCTTCCGGGCTTGCTCAAGACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCTGTATCTCCCACCACATCC                                                                                                                               <  SRR19973066.471564/150‑1 (MQ=60)
                       AGCGACTGACGGGCATCGTCGATCAGATCCCGGGCTTTCTTCCGGGCTTGCTCAAGACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCTGTATCTCCCACCACATCCACGATG                                                                                                                         >  SRR19973066.1122485/1‑150 (MQ=60)
                                    CATCGTCGATCAGATCCCGGGCTTTCTTCCGGGCTTGCTCAAGACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCTGTATCTCCCACCACATCCAGGATGTCATCCTGAACCT                                                                                                            <  SRR19973066.3564106/150‑1 (MQ=60)
                                          CGATCAGATCCCGGGCTTTCTTCCGGGCTTGCTCAAGACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTTCCAACCTTGCTGTATATCCCACaacaaacacgatctcaacctgaaacaggaagg                                                                                                      >  SRR19973066.1832842/1‑118 (MQ=60)
                                           GATCAGATCCCGGGCTTTCTTCCGGGCTTGCTCAAGACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCTGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGC                                                                                                     <  SRR19973066.1041313/150‑1 (MQ=60)
                                           GATCAGATCCCGGGCTTTCTTCCGGGCTTGCTCAAGACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCTGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGC                                                                                                     <  SRR19973066.1043000/150‑1 (MQ=60)
                                              CAGATCCCGGGCTTTCTTCCGGGCTTGCTCAAGACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCTGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAG                                                                                                  <  SRR19973066.699558/150‑1 (MQ=60)
                                                  TCCCGGGCTTTCTTCCGGGCTTGCTCAAGACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCTGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCG                                                                                              <  SRR19973066.1367889/150‑1 (MQ=60)
                                                    CCGGGCTTTCTTCCGGGCTTGCTCAAGACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCTGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGAT                                                                                            <  SRR19973066.2147906/150‑1 (MQ=60)
                                                    CCGGGCTTTCTTCCGGGCTTGCTCAAGACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCTGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGAT                                                                                            <  SRR19973066.2275718/150‑1 (MQ=60)
                                                    CCGGGCTTTCTTCCGGGCTTGCTCAAGACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCTGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGAT                                                                                            <  SRR19973066.408358/150‑1 (MQ=60)
                                                       GGCTTTCTTCCGGGCTTGCTCAAGACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCCACGTTGCTGTATCTCCCACCCCAACCACGATGTCATCCTGAAACTGGAAGGCAAGGCCGATGCT                                                                                         >  SRR19973066.3411022/1‑150 (MQ=60)
                                                         CTTTCTTCCGGGCTTGCTCAAGACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCTGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCT                                                                                       <  SRR19973066.3090547/150‑1 (MQ=60)
                                                                CCGGGCTTGCTCAAGACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCTGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATA                                                                                <  SRR19973066.1397332/150‑1 (MQ=60)
                                                                 CGGGCTTGCTCAAGACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCTGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATAC                                                                               >  SRR19973066.1513595/1‑150 (MQ=60)
                                                                     CTTGCTCAAGACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCTGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGT                                                                           <  SRR19973066.2534262/150‑1 (MQ=60)
                                                                       TGCTCAAGACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGGTGCTGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCG                                                                         >  SRR19973066.1770872/1‑150 (MQ=60)
                                                                           CAAGACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCTGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTA                                                                     <  SRR19973066.3512870/150‑1 (MQ=60)
                                                                                CCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCTGTATCTCCCACCACATACAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCGGC                                                                >  SRR19973066.2661235/1‑150 (MQ=60)
                                                                                  CAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACCTTGCTGTAACTACCACCACAAACAGGATGTCATCCTGAACCTGGGAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCGGCAG                                                              >  SRR19973066.1308221/1‑150 (MQ=60)
                                                                                   AGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGGTTCTGTATATACCACCACATCCAGGATGTCATACTGAAACTGGAAGGCAACGCCGATGCTCTCTGCATAATTTTCTAGTAACGGCAGA                                                             >  SRR19973066.2378678/1‑150 (MQ=60)
                                                                                      AGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCTGTATCTCCCACCACATCCAGGATGTGCTCCTGAAACAGGAAGGCAACGCCGATGCTCTCTGCATACTTGTCGAGTACCGGCAGAGCA                                                          >  SRR19973066.519249/1‑150 (MQ=60)
                                                                                          CAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCTGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCGGCAGAGCACGAC                                                      <  SRR19973066.2266723/150‑1 (MQ=60)
                                                                                           AGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACCTTGCGGTATCTCCCACCACAACCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATTCTCTCTGCAAAAATGTCGAGTAACCGCAGAGCACGACG                                                     >  SRR19973066.2860077/1‑150 (MQ=60)
                                                                                               TAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTGTTGCCCACGTTTCTGTATATCCCACCACAACCACGATGTCATACTGAACCGGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCGGCCGCGCACCACGTCCT                                                 >  SRR19973066.317931/1‑150 (MQ=60)
                                                                                                AGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCACCGTTGCTGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCGGCAGAGCACGACGTCCTT                                                <  SRR19973066.1703809/150‑1 (MQ=60)
                                                                                                AGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCACCGTTGCTGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCGGCAGAGCACGACGTCCTT                                                <  SRR19973066.1639762/150‑1 (MQ=60)
                                                                                                AGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCTGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCGGCAGAGCACGACGTCCTT                                                <  SRR19973066.1639689/150‑1 (MQ=60)
                                                                                                AGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCTGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCGGCAGAGCACGACGTCCTT                                                <  SRR19973066.3386581/150‑1 (MQ=60)
                                                                                                AGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCTGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCGGCAGAGCACGACGTCCTT                                                <  SRR19973066.2161101/150‑1 (MQ=60)
                                                                                                 GGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTGCCAACGTTTGTGTATCTCCCACCACAACCACGATGTGAACCTGAACCTGGAAGGCACCGCCGATGCGCTCTGGATAATTTTGGAGTACCGGCAGAGCAACACGTCCTTT                                               >  SRR19973066.984959/1‑150 (MQ=60)
                                                                                                  GTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGGTGCTGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGCTGCTCTCTGCATACTTGTCGAGGACCGGCAGAGCACGACGTCCTTTA                                              >  SRR19973066.3098569/1‑150 (MQ=60)
                                                                                                  GTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGGTGCTGTATCTCCCACCACAACCACGATGTCATCCTGAACCAGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCGGCAGAGCACGACGTCCGTTA                                              >  SRR19973066.3097808/1‑150 (MQ=60)
                                                                                                    ACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGGTGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCGGCCGAGCACGACGTCCTTTATC                                            >  SRR19973066.1460007/1‑150 (MQ=60)
                                                                                                    ACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCTGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATC                                            >  SRR19973066.1460060/1‑150 (MQ=60)
                                                                                                     CTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAAAGTTGCTGTATCTCCCACCACATCCCGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTAACGGCAGAGCACGACGTCCTTTATCT                                           >  SRR19973066.2374267/1‑150 (MQ=60)
                                                                                                      TTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCTGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTC                                          <  SRR19973066.3107930/150‑1 (MQ=60)
                                                                                                      TTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCTGTATCTCCCACCACAACCCGGATGTCATACAGAACCTGGAAGGCAAGGCCGATGCTCTCAGCATAATTGTCGAGTACCCGCAGAGCACCACGTCCATTATCTC                                          >  SRR19973066.1956585/1‑150 (MQ=60)
                                                                                                             AAGTTGCTGGTCGGCACCCTGGCGTTTTGCCCACCTTGGTGTATATCCCACCACATCCAGGATGTAAAACTGAACCAGGGAGGCAAGGCCGCAGCTCTCTGCCTAAATGTCGAGTAACGGCCGAGCACGACGTCCgttataaccggcgcg                                   >  SRR19973066.1053522/1‑135 (MQ=33)
                                                                                                             AAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGGTGCGGTATCTACCACCACATCCAGGATGTGATCCTGAACCAGGAAGGCACGGCCGATGCTCTCTGCATACTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCGGCGCT                                   >  SRR19973066.357388/1‑150 (MQ=60)
                                                                                                              AGTTGCTGGTCGGCACCCTGGCGTTTTCCCCACGTTGGGGTATCTCCCACCACAACCAGGCTGTCATCCGGAACCTGGAAGGCCAGGCCGCTGCTCTCTGCATACATGTGGAGTACCGGCCGAGCACGACGTCCGGTATCTCCGGCGCGT                                  >  SRR19973066.1021371/1‑150 (MQ=60)
                                                                                                              AGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCTGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATATCCGGCGCTT                                  >  SRR19973066.2059965/1‑150 (MQ=60)
                                                                                                                 TGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCTGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCGGCGCTTAAT                               <  SRR19973066.424520/150‑1 (MQ=60)
                                                                                                                 TGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCTGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCGGCGCTTAAT                               <  SRR19973066.466896/150‑1 (MQ=60)
                                                                                                                   CTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCTGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCGGCGCTTAATGC                             <  SRR19973066.1191053/150‑1 (MQ=60)
                                                                                                                           CACCCTGGCGTTTTCCCAACGTTGCTGTATCTCCCACCACATCCAGGATGTCATCCTGAAACTGGAAGGCAAGGCCGATGCTCTCTGCATAATTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCGGCGCTTAATTCACCAAGGC                     >  SRR19973066.588323/1‑150 (MQ=60)
                                                                                                                           CACCCTGGCGTTTTCCCAACGTTGCTGTATCTCCCACCACATACAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCGGCGCTTAATGCACCAAGGC                     >  SRR19973066.588250/1‑150 (MQ=60)
                                                                                                                            ACCCTGGCGTTTTCCCAACGTTGCTGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCGGCGCTTAATGCACCAAGGCG                    <  SRR19973066.105743/150‑1 (MQ=60)
                                                                                                                              CCTGGCGTTTTCCCAACGTTGCTGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCGGCGCTTAATGCACCAAGGCGAA                  >  SRR19973066.1509438/1‑150 (MQ=60)
                                                                                                                              CCTGGCGTTTTCCCAACGTTGCAGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCGGCGCTTAATGCACCAAGGCGAA                  <  SRR19973066.335862/150‑1 (MQ=60)
                                                                                                                               CTGGCGTTTTCCCAACGTTGCTGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCGGCGCTTAATGCACCAAGGCGAAC                 >  SRR19973066.3069231/1‑150 (MQ=60)
                                                                                                                               CTGGCGTTTTCCCAACGTTGCTGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCGGCGCTTAATGCACCAAGGCGAAC                 >  SRR19973066.3068782/1‑150 (MQ=60)
                                                                                                                                  GCGTTTTCCCAACGTTGCTGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCGGCGCTTAATGCACCAAGGCGAACGGC              <  SRR19973066.1583300/150‑1 (MQ=60)
                                                                                                                                      TTTCCCAACGTTGCTGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCGGCGCTTAATGCACCAAGGCGAACGGCGGCG          <  SRR19973066.1484869/150‑1 (MQ=60)
                                                                                                                                        TCCCAACGTTGCTGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCGGCGCTTAATGCACCAAGGCGAACGGCGGCGCG        <  SRR19973066.3185329/150‑1 (MQ=60)
                                                                                                                                              CGTTGCTGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCGGCGCTTAATGCACCAAGGCGAACGGCGGCGCGAATCAA  <  SRR19973066.1753911/150‑1 (MQ=60)
                                                                                                                                                    |                                                                                                                                               
GTGACTGTTCAGCCAGTTGTTTCAGCGACTGACGGGCATCGTCGATCAGATCCCGGGCTTTCTTCCGGGCTTGCTCAAGACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCAGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCGGCGCTTAATGCACCAAGGCGAACGGCGGCGCGAATCAA  >  CP009273/435714‑436005

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑