Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F394 I0 R1 1342 215.0 7134094 96.2% 6862998 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,723,809 G→T 100% E156* (GAA→TAA)  lhr → putative ATP‑dependent helicase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,723,8090GT95.9% 579.0 / 14.5 198E156* (GAA→TAA) lhrputative ATP‑dependent helicase
Reads supporting (aligned to +/- strand):  ref base G (8/0);  new base T (88/102);  total (96/102)
Fisher's exact test for biased strand distribution p-value = 2.61e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.31e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 198 total aligned reads displayed.

CCCGTTGAAGGGTATTGCCGATGAACGGCGGCGGCGCGGCGAAACGGAAGTCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCGAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTAGCGGGCAGTAAACGTGGTGCGCATCTGGCGTTAAGTCTGGAGCGGCTCGATGCGCTGC  >  CP009273/1723664‑1723953
                                                                                                                                                 |                                                                                                                                                
cccGTTGAAGGGTATTGCCGATGAACGGCGGCGGCGCGGCGAAACGGAAGTCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCGaa                                                                                                                                                >  1:1590576/1‑148 (MQ=255)
cccGTTGAAGGGTATTGCCGATGAACGGCGGCGGCGCGGCGAAACGGAAGTCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCGaa                                                                                                                                                >  1:1590850/1‑148 (MQ=255)
 ccGTTGAAGGGTATTGCCGATGAACGGCGGCGGCGCGGCGAAACGGAAGTCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAAt                                                                                                                                               <  2:1778956/148‑1 (MQ=255)
 ccGTTGAAGGGTATTGCCGATGAACGGCGGCGGCGCGGCGAAACGGAAGTCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAAt                                                                                                                                               >  1:3051348/1‑148 (MQ=255)
     tGAAGGGTATTGCCGATGAACGGCGGCGGCGCGGCGAAACGGAAGTCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCAct                                                                                                                                           >  2:2539178/1‑148 (MQ=255)
     tGAAGGGTATTGCCGATGAACGGCGGCGGCGCGGCGAAACGGAAGTCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCAct                                                                                                                                           >  1:3342954/1‑148 (MQ=255)
     tGAAGGGTATTGCCGATGAACGGCGGCGGCGCGGCGAAACGGAAGTCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCAct                                                                                                                                           <  2:2094680/148‑1 (MQ=255)
       aaGGGTATTGCCGATGAACGGCGGCGGCGCGGCGAAACGGAAGTCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATCTTCTGATCACCACACCCTAATCActct                                                                                                                                         >  1:2813509/1‑148 (MQ=255)
          ggTATTGCCGATGAACGGCGGCGGCGCGGCGAAACGGAAGTCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATc                                                                                                                                      <  2:3460276/148‑1 (MQ=255)
            tATTGCCGATGAACGGCGGCGGCGCGGCGAAACGGAAGTCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTg                                                                                                                                    <  2:2791759/148‑1 (MQ=255)
            tATTGCCGATGAACGGCGGCGGCGCGGCGAAACGGAAGTCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTg                                                                                                                                    <  2:3278730/148‑1 (MQ=255)
             aTTGCCGATGAACGGCGGCGGCGCGGCGAAACGGAAGTCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGa                                                                                                                                   <  1:3171008/148‑1 (MQ=255)
               tGCCGATGAACGGCGGCGGCGCGGCGAAACGGAAGTCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATg                                                                                                                                 <  2:3027515/148‑1 (MQ=255)
               tGCCGATGAACGGCGGCGGCGCGGCGAAACGGAAGTCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATg                                                                                                                                 >  2:257845/1‑148 (MQ=255)
                  cGATGAACGGCGGCGGCGCGGCGAAACGGAAGTCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTg                                                                                                                              <  1:3286116/148‑1 (MQ=255)
                     tGAACGGCGGCGGCGCGGCGAAACGGAAGTCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGAcc                                                                                                                           >  1:2866594/1‑148 (MQ=255)
                     tGAACGGCGGCGGCGCGGCGAAACGGAAGTCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGAcc                                                                                                                           <  2:1269938/148‑1 (MQ=255)
                     tGAACGGCGGCGGCGCGGCGAAACGGAAGTCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGAcc                                                                                                                           >  1:1088015/1‑148 (MQ=255)
                        aCGGCGGCGGCGCGGCGAAACGGAAGTCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTcc                                                                                                                        <  1:1942833/148‑1 (MQ=255)
                         cggcggcggCGCGGCGAAACGGAAGTCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTccc                                                                                                                       >  2:1702494/1‑148 (MQ=255)
                         cggcggcggCGCGGCGAAACGGAAGTCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTccc                                                                                                                       >  2:925948/1‑148 (MQ=255)
                                 gcgcggcgAAACGGAAGTCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCcgcgcgcgc                                                                                                               <  1:16932/148‑1 (MQ=255)
                                  cgcggcgAAACGGAAGTCAATCTTCGCGTAGGTATCCGTACTGGCGATACGACTGCACAGGAACGCAGCAAACTCTCCCGTAATCCGCCGGATATTCTTATAACCACACCCTATTCACTCTATCTGATGCTGACCTCCcgcgcgcgcg                                                                                                              <  2:371909/148‑1 (MQ=255)
                                  cgcggcgAAACGGAAGTCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCcgcgcgcgcg                                                                                                              <  2:2166693/148‑1 (MQ=255)
                                    cggcgAAACGGAAGTCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGaa                                                                                                            <  1:2309347/148‑1 (MQ=255)
                                       cgAAACGGAAGTCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACg                                                                                                         <  1:2439675/148‑1 (MQ=255)
                                       cgAAACGGAAGTCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACg                                                                                                         <  1:1729928/148‑1 (MQ=255)
                                         aaaCGGAAGTCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCt                                                                                                       <  1:2528501/148‑1 (MQ=255)
                                         aaaCGGAAGTCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCt                                                                                                       <  2:270900/148‑1 (MQ=255)
                                         aaaCGGAAGTCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCt                                                                                                       >  2:3011880/1‑148 (MQ=255)
                                           aCGGAAGTCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTAc                                                                                                     >  1:2038733/1‑148 (MQ=255)
                                             ggAAGTCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTAcgc                                                                                                   <  2:1733906/148‑1 (MQ=255)
                                               aaGTCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACgcgg                                                                                                 >  1:659052/1‑148 (MQ=255)
                                               aaGTCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACgcgg                                                                                                 >  2:1293013/1‑148 (MQ=255)
                                               aaGTCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACgcgg                                                                                                 >  2:2901274/1‑148 (MQ=255)
                                                  tCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGt                                                                                              <  2:1712075/148‑1 (MQ=255)
                                                      tCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGaa                                                                                          <  2:536590/148‑1 (MQ=255)
                                                      tCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGaa                                                                                          <  1:612988/148‑1 (MQ=255)
                                                      tCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGaa                                                                                          <  1:222259/148‑1 (MQ=255)
                                                       cTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGaaa                                                                                         >  2:1241368/1‑148 (MQ=255)
                                                         tCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACg                                                                                       <  2:3408406/148‑1 (MQ=255)
                                                           gcgTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGt                                                                                     <  2:2050702/148‑1 (MQ=255)
                                                            cgTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTa                                                                                    >  2:949535/1‑148 (MQ=255)
                                                              tAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAat                                                                                  >  2:2033409/1‑148 (MQ=255)
                                                                 ggATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAattat                                                                               <  1:1732530/148‑1 (MQ=255)
                                                                   aTCCGTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATtg                                                                             <  1:2598735/148‑1 (MQ=255)
                                                                   aTCCGTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATtg                                                                             >  2:1231128/1‑148 (MQ=255)
                                                                   aTCCGTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATtg                                                                             <  2:1417795/148‑1 (MQ=255)
                                                                    tCCGTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATtga                                                                            >  2:1623829/1‑148 (MQ=255)
                                                                       gTACTGTCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTAAGCGGCGTCTAAAAGGTAATTATtgatga                                                                         <  2:2919641/148‑1 (MQ=255)
                                                                       gTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTCTCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATtgatga                                                                         <  1:71438/148‑1 (MQ=255)
                                                                       gTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATtgatga                                                                         <  1:2718593/148‑1 (MQ=255)
                                                                        tACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGaa                                                                        <  1:1314949/148‑1 (MQ=255)
                                                                         aCTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAg                                                                       <  2:2825505/148‑1 (MQ=255)
                                                                            ggCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTcc                                                                    <  1:844485/148‑1 (MQ=255)
                                                                                aTACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCAcgc                                                                >  1:1505213/1‑148 (MQ=255)
                                                                                aTACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCAcgc                                                                >  2:1917361/1‑148 (MQ=255)
                                                                                  aCGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCgg                                                              >  2:1253153/1‑148 (MQ=255)
                                                                                  aCGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCgg                                                              >  2:1253253/1‑148 (MQ=255)
                                                                                  aCGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCgg                                                              <  1:3132858/148‑1 (MQ=255)
                                                                                   cGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGt                                                             <  1:3502379/148‑1 (MQ=255)
                                                                                    gCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTa                                                            <  1:257845/148‑1 (MQ=255)
                                                                                       tGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTAGCg                                                         <  2:796547/148‑1 (MQ=255)
                                                                                        gCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTACTCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTAGCgg                                                        <  2:151740/148‑1 (MQ=255)
                                                                                        gCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCGAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTAGCgg                                                        >  1:571990/1‑148 (MQ=255)
                                                                                         cacaGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTAGCggg                                                       >  1:2159203/1‑148 (MQ=255)
                                                                                          acaGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTAGCGGGc                                                      <  2:1088015/148‑1 (MQ=255)
                                                                                             ggAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTAGCGGGCAGt                                                   >  1:3216753/1‑148 (MQ=255)
                                                                                               aaCGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTAGCGGGCAGTaa                                                 >  2:3488338/1‑148 (MQ=255)
                                                                                               aaCGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTAGCGGGCAGTaa                                                 >  2:1724601/1‑148 (MQ=255)
                                                                                                aCGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTAGCGGGCAGTaaa                                                <  2:2490093/148‑1 (MQ=255)
                                                                                                 cGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTAGCGGGCAGTAAAc                                               >  2:573793/1‑148 (MQ=255)
                                                                                                   cagcaAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTAGCGGGCAGTAAACgt                                             <  2:1022540/148‑1 (MQ=255)
                                                                                                    agcaAACTCACCCGTAATCCGCCGGATATACTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTAGCGGGCAGTAAACgtg                                            <  2:1396126/148‑1 (MQ=255)
                                                                                                     gcaAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTAGCGGGCAGTAAACgtgg                                           >  1:461930/1‑148 (MQ=255)
                                                                                                      caAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTAGCGGGCAGTAAACgtggt                                          >  2:2531326/1‑148 (MQ=255)
                                                                                                      caAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTAGCGGGCAGTAAACgtggt                                          >  1:2755382/1‑148 (MQ=255)
                                                                                                            cACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTAGCGGGCAGTAAACGTGGTGCGCAt                                    <  1:3005787/148‑1 (MQ=255)
                                                                                                             aCCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTAGCGGGCAGTAAACGTGGTGCGCATc                                   >  2:3123895/1‑148 (MQ=255)
                                                                                                              cccGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTAGCGGGCAGTAAACGTGGTGCGCATCt                                  <  1:1241368/148‑1 (MQ=255)
                                                                                                              cccGTAATCCGCCGGATATTCTGATCACCACACCCGAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTAGCGGGCAGTAAACGTGGTGCGCATCt                                  >  1:1466817/1‑148 (MQ=255)
                                                                                                               ccGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTAGCGGGCAGTAAACGTGGTGCGCATCTg                                 >  1:1522487/1‑148 (MQ=255)
                                                                                                                    aTCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTAGCGGGCAGTAAACGTGGTGCGCATCTGGCGtt                            >  1:971130/1‑148 (MQ=255)
                                                                                                                         ccgGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTAGCGGGCAGTAAACGTGGTGCGCATCTGGCGTTAAGTc                       >  2:1667724/1‑148 (MQ=255)
                                                                                                                         ccgGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTAGCGGGCAGTAAACGTGGTGCGCATCTGGCGTTAAGTc                       >  1:257670/1‑148 (MQ=255)
                                                                                                                         ccgGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCGACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTAGCGGGCAGTAAACGTGGTGCGCATCTGGCGTTAAGTc                       <  2:1492603/148‑1 (MQ=255)
                                                                                                                           ggATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTAGCGGGCAGTAAACGTGGTGCGCATCTGGCGTTAAGTCTg                     >  1:1705073/1‑148 (MQ=255)
                                                                                                                           ggATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTAGCGGGCAGTAAACGTGGTGCGCATCTGGCGTTAAGTCTg                     <  2:3212876/148‑1 (MQ=255)
                                                                                                                           ggATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTAGCGGGCAGTAAACGTGGTGCGCATCTGGCGTTAAGTCTg                     >  1:1854231/1‑148 (MQ=255)
                                                                                                                            gATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTAGCGGGCAGTAAACGTGGTGCGCATCTGGCGTTAAGTCTgg                    <  2:2309179/148‑1 (MQ=255)
                                                                                                                            gATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTAGCGGGCAGTAAACGTGGTGCGCATCTGGCGTTAAGTCTgg                    >  2:2426725/1‑148 (MQ=255)
                                                                                                                             atatTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTAGCGGGCAGTCAACGTGGTGCGCATCTGGCGTTAAGTCTGGa                   >  2:3043714/1‑148 (MQ=255)
                                                                                                                                 tCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTAGCGGGCAGTAAACGTGGTGCGCATCTGGCGTTAAGTCTGGAGCgg               >  1:2895574/1‑148 (MQ=255)
                                                                                                                                 tCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTAGCGGGCAGTAAACGTGGTGCGCATCTGGCGTTAAGTCTGGAGCgg               <  1:2033409/148‑1 (MQ=255)
                                                                                                                                 tCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTAGCGGGCAGTAAACGTGGTGCGCATCTGGCGTTAAGTCTGGAGCgg               >  2:2634527/1‑148 (MQ=255)
                                                                                                                                 tCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTAGCGGGCAGTAAACGTGGTGCGCATCTGGCGTTAAGTCTGGAGCgg               >  1:519117/1‑148 (MQ=255)
                                                                                                                                  cTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTAGCGGGCAGTAAACGTGGTGCGCATCTGGCGTTAAGTCTGGAGCGGc              <  2:109732/148‑1 (MQ=255)
                                                                                                                                      tCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTAGCGGGCAGTAAACGTGGTGCGCATCTGGCGTTAAGTCTgg                    <  2:2878872/138‑1 (MQ=255)
                                                                                                                                           acacCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTAGCGGGCAGTAAACGTGGTGCGCATCTGGCGTTAAGTCTGGAGCGGCTCGATgcgc     >  1:3179134/1‑148 (MQ=255)
                                                                                                                                              cccTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTAGCGGGCAGTAAACGTGGTGCGCATCTGGCGTTAAGTCTGGAGCGGCTCGATGCgctgc  >  2:695467/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
CCCGTTGAAGGGTATTGCCGATGAACGGCGGCGGCGCGGCGAAACGGAAGTCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCGAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTAGCGGGCAGTAAACGTGGTGCGCATCTGGCGTTAAGTCTGGAGCGGCTCGATGCGCTGC  >  CP009273/1723664‑1723953

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GATCCCGTTGAAGGGTATTGCCGATGAACGGCGGCGGCGCGGCGAAACGGAAGTCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCGAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTAGCGGGCAGTAAACGTGGTGCGCATCTGGCGTTAAGTCTGGAGCGGCTCGATGCGCTGCTCCA  >  CP009273/1723661‑1723957
                                                                                                                                                    |                                                                                                                                                    
GATCCCGTTGAAGGGTATTGCCGATGAACGGCGGCGGCGCGGCGAAACGGAAGTCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTA                                                                                                                                                     <  SRR19973066.1761087/150‑1 (MQ=60)
GATCCCGTTGAAGGGTATTGCCGATGAACGGCGGCGGCGCGGCGAAACGGAAGTCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTA                                                                                                                                                     <  SRR19973066.1761099/150‑1 (MQ=60)
GATCCCGTTGAAGGGTATTGCCGATGAACGGCGGCGGCGCGGCGAAACGGAAGTCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTA                                                                                                                                                     <  SRR19973066.3101739/150‑1 (MQ=60)
GATCCCGTTGAAGGGTATTGCCGATGAACGGCGGCGGCGCGGCGAAACGGAAGTCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTA                                                                                                                                                     <  SRR19973066.3101339/150‑1 (MQ=60)
GATCCCGTTGAAGGGTATTGCCGATGAACGGCGGCGGCGCGGCGAAACGGAAGTCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTA                                                                                                                                                     <  SRR19973066.3101122/150‑1 (MQ=60)
 ATCCCGTTGAAGGGTATTGCCGATGAACGGCGGCGGCGCGGCGAAACGGAAGTCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCGAA                                                                                                                                                    >  SRR19973066.1602087/1‑150 (MQ=60)
 ATCCCGTTGAAGGGTATTGCCGATGAACGGCGGCGGCGCGGCGAAACGGAAGTCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCGAA                                                                                                                                                    >  SRR19973066.1602367/1‑150 (MQ=60)
 ATCCCGTTGAAGGGTATTGCCGATGAACGGCGGCGGCGCGGCGAAACGGAAGTCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCGAA                                                                                                                                                    >  SRR19973066.1602329/1‑150 (MQ=60)
  TCCCGTTGAAGGGTATTGCCGATGAACGGCGGCGGCGCGGCGAAACGGAAGTCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAAT                                                                                                                                                   >  SRR19973066.3071755/1‑150 (MQ=60)
   CCCGTTGAAGGGTATTGCCGATGAACGGCGGCGGCGCGGCGAAACGGAAGTCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATC                                                                                                                                                  >  SRR19973066.2259118/1‑150 (MQ=60)
   CCCGTTGAAGGGTATTGCCGATGAACGGCGGCGGCGCGGCGAAACGGAAGTCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATC                                                                                                                                                  <  SRR19973066.2026697/150‑1 (MQ=60)
     CGTTGAAGGGTATTGCCGATGAACGGCGGCGGCGCGGCGAAACGGAAGTCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCAC                                                                                                                                                >  SRR19973066.1531833/1‑150 (MQ=60)
      GTTGAAGGGTATTGCCGATGAACGGCGGCGGCGCGGCGAAACGGAAGTCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACT                                                                                                                                               >  SRR19973066.3365209/1‑150 (MQ=60)
        TGAAGGGTATTGCCGATGAACGGCGGCGGCGCGGCGAAACGGAAGTCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATCTTCTGATCACCACACCCTAATCACTCT                                                                                                                                             >  SRR19973066.2832431/1‑150 (MQ=60)
        TGAAGGGTATTGCCGATGAACGGCGGCGGCGCGGCGAAACGGAAGTCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCT                                                                                                                                             >  SRR19973066.2832291/1‑150 (MQ=60)
            GGGTATTGCCGATGAACGGCGGCGGCGCGGCGAAACGGAAGTCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCT                                                                                                                                         >  SRR19973066.3309545/1‑150 (MQ=60)
             GGTATTGCCGATGAACGGCGGCGGCGCGGCGAAACGGAAGTCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTG                                                                                                                                        <  SRR19973066.557924/150‑1 (MQ=60)
                ATTGCCGATGAACGGCGGCGGCGCGGCGAAACGGAAGTCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATG                                                                                                                                     <  SRR19973066.3192126/150‑1 (MQ=60)
                ATTGCCGATGAACGGCGGCGGCGCGGCGAAACGGAAGTCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATG                                                                                                                                     <  SRR19973066.2174856/150‑1 (MQ=60)
                     CGATGAACGGCGGCGGCGCGGCGAAACGGAAGTCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGAC                                                                                                                                <  SRR19973066.3307763/150‑1 (MQ=60)
                      GATGAACGGCGGCGGCGCGGCGAAACGGAAGTCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACC                                                                                                                               >  SRR19973066.2885827/1‑150 (MQ=60)
                      GATGAACGGCGGCGGCGCGGCGAAACGGAAGTCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACC                                                                                                                               >  SRR19973066.1095951/1‑150 (MQ=60)
                           ACGGCGGCGGCGCGGCGAAACGGAAGTCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCG                                                                                                                          <  SRR19973066.1956604/150‑1 (MQ=60)
                                    GCGCGGCGAAACGGAAGTCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGA                                                                                                                 <  SRR19973066.17194/150‑1 (MQ=60)
                                       CGGCGAAACGGAAGTCTATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTTCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCGGATGCTGACCTCCCGCGCGCGCGAACC                                                                                                              <  SRR19973066.2836857/150‑1 (MQ=60)
                                       CGGCGAAACGGAAGTCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAAC                                                                                                              <  SRR19973066.2325156/150‑1 (MQ=60)
                                        GGCGAAACGGAAGTCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACG                                                                                                             >  SRR19973066.2751395/1‑150 (MQ=60)
                                          CGAAACGGAAGTCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCT                                                                                                           <  SRR19973066.2456293/150‑1 (MQ=60)
                                          CGAAACGGAAGTCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCT                                                                                                           <  SRR19973066.1742428/150‑1 (MQ=60)
                                          CGAAACGGAAGTCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCT                                                                                                           <  SRR19973066.1742427/150‑1 (MQ=60)
                                            AAACGGAAGTCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTAC                                                                                                         >  SRR19973066.2052940/1‑150 (MQ=60)
                                            AAACGGAAGTCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTAC                                                                                                         <  SRR19973066.2545693/150‑1 (MQ=60)
                                                GGAAGTCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTCCGCGG                                                                                                     >  SRR19973066.1708363/1‑150 (MQ=60)
                                                GGAAGTCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGG                                                                                                     >  SRR19973066.944891/1‑150 (MQ=60)
                                                GGAAGTCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGG                                                                                                     >  SRR19973066.663864/1‑150 (MQ=60)
                                                   AGTCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGT                                                                                                  <  SRR19973066.1807149/150‑1 (MQ=60)
                                                         TCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAAC                                                                                            <  SRR19973066.617271/150‑1 (MQ=60)
                                                         TCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAAC                                                                                            <  SRR19973066.224066/150‑1 (MQ=60)
                                                                  AGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTAT                                                                                   <  SRR19973066.2595772/150‑1 (MQ=60)
                                                                    GGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTG                                                                                 <  SRR19973066.1745043/150‑1 (MQ=60)
                                                                    GGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTG                                                                                 <  SRR19973066.1744825/150‑1 (MQ=60)
                                                                    GGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTG                                                                                 <  SRR19973066.2079550/150‑1 (MQ=60)
                                                                      ATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGAT                                                                               <  SRR19973066.2616325/150‑1 (MQ=60)
                                                                          GTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTCTCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAG                                                                           <  SRR19973066.72293/150‑1 (MQ=60)
                                                                          GTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAG                                                                           <  SRR19973066.2736871/150‑1 (MQ=60)
                                                                          GTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAG                                                                           <  SRR19973066.2735319/150‑1 (MQ=60)
                                                                           TACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGT                                                                          <  SRR19973066.1324483/150‑1 (MQ=60)
                                                                            ACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCAATCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTC                                                                         >  SRR19973066.3481093/1‑150 (MQ=60)
                                                                               GGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCAC                                                                      <  SRR19973066.2562600/150‑1 (MQ=60)
                                                                               GGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCAC                                                                      <  SRR19973066.850616/150‑1 (MQ=60)
                                                                                 CGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGC                                                                    >  SRR19973066.1515696/1‑150 (MQ=60)
                                                                                     ACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTA                                                                <  SRR19973066.3153769/150‑1 (MQ=60)
                                                                                      CGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTACTCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTAG                                                               <  SRR19973066.3552305/150‑1 (MQ=60)
                                                                                      CGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTAG                                                               <  SRR19973066.3525730/150‑1 (MQ=60)
                                                                                       GCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTAGC                                                              <  SRR19973066.259792/150‑1 (MQ=60)
                                                                                         CTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCGAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTAGCGG                                                            >  SRR19973066.576031/1‑150 (MQ=60)
                                                                                          TGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTAGCGGG                                                           >  SRR19973066.2174199/1‑150 (MQ=60)
                                                                                          TGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTAGCGGG                                                           <  SRR19973066.320068/150‑1 (MQ=60)
                                                                                             ACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTAGCGGGCAG                                                        >  SRR19973066.2468553/1‑150 (MQ=60)
                                                                                              CAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTAGCGGGCAGT                                                       >  SRR19973066.3238048/1‑150 (MQ=60)
                                                                                                  AACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTAGCGGGCAGTAAAC                                                   >  SRR19973066.620206/1‑150 (MQ=60)
                                                                                                  AACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTAGCGGGCAGTAAAC                                                   >  SRR19973066.683076/1‑150 (MQ=60)
                                                                                                      CAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTAGCGGGCAGTAAACGTGG                                               >  SRR19973066.465463/1‑150 (MQ=60)
                                                                                                      CAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATAATTGATGAAGTCCACGCGGTAGCGGGCAGTAAACGTGG                                               >  SRR19973066.465476/1‑150 (MQ=60)
                                                                                                       AGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTAGCGGGCAGTAAACGTGGT                                              <  SRR19973066.3293112/150‑1 (MQ=60)
                                                                                                       AGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTAGCGGGCAGTAAACGTGGT                                              >  SRR19973066.2773935/1‑150 (MQ=60)
                                                                                                         CAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTAGCGGGCAGTAAACGTGGTGC                                            <  SRR19973066.3151098/150‑1 (MQ=60)
                                                                                                               CACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTAGCGGGCAGTAAACGTGGTGCGCATCT                                      <  SRR19973066.3025795/150‑1 (MQ=60)
                                                                                                               CACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTAGCGGGCAGTAAACGTGGTGCGCATCT                                      <  SRR19973066.3025778/150‑1 (MQ=60)
                                                                                                               CACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTAGCGGGCAGTAAACGTGGTGCGCATCT                                      <  SRR19973066.3025700/150‑1 (MQ=60)
                                                                                                               CACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTAGCGGGCAGTAAACGTGGTGCGCATCT                                      >  SRR19973066.612023/1‑150 (MQ=60)
                                                                                                               CACCCGTAATCCGCCGGATATTCTGATCACCACACCCGAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTAGCGGGCAGTAAACGTGGTGCGCATCT                                      >  SRR19973066.1477169/1‑150 (MQ=60)
                                                                                                               CACCCGTAATCCGCCGGATATTCTGATCACCACACCCGAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTAGCGGGCAGTAAACGTGGTGCGCATCT                                      >  SRR19973066.1477396/1‑150 (MQ=60)
                                                                                                                ACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTAGCGGGCAGTAAACGTGGTGCGCATCTG                                     >  SRR19973066.1533241/1‑150 (MQ=60)
                                                                                                                ACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTAGCGGGCAGTAAACGTGGTGCGCATCTG                                     >  SRR19973066.1533264/1‑150 (MQ=60)
                                                                                                                 CCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTAGCGGGCAGTAAACGTGGTGCGCATCTGG                                    <  SRR19973066.1250248/150‑1 (MQ=60)
                                                                                                                   CGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCTCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGAGGTAGCGGGCAGTAAACGTGGTGCGCATCTGGCG                                  >  SRR19973066.2606152/1‑150 (MQ=60)
                                                                                                                     TAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTAGCGGGCAGTAAACGTGGTGCGCATCTGGCGTT                                >  SRR19973066.978272/1‑150 (MQ=60)
                                                                                                                        TCCGCCGGATATTCTGATCACCACACCCTACTCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTAGCGGGCAGTAAACGTGGTGCGCATCTGGCGTTAAG                             <  SRR19973066.3170390/150‑1 (MQ=60)
                                                                                                                          CGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTAGCGGGCAGTAAACGTGGTGCGCATCTGGCGTTAAGTC                           >  SRR19973066.259617/1‑150 (MQ=60)
                                                                                                                            CCGTATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTAGCGGGCAGTAAACGTGGTGCGCATCTGTCGTTAAGTCTG                         >  SRR19973066.1867633/1‑150 (MQ=60)
                                                                                                                            CCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTAGCGGGCAGTAAACGTGGTGCGCATCTGGCGTTAAGTCTG                         >  SRR19973066.1717451/1‑150 (MQ=60)
                                                                                                                            CCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTAGCGGGCAGTAAACGTGGTGCGCATCTGGCGTTAAGTCTG                         >  SRR19973066.1867499/1‑150 (MQ=60)
                                                                                                                                 TATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTAGCGGGCAGTAAACGTGGTGCGCATCTGGCGTTAAGTCTGGAGCG                    >  SRR19973066.3216281/1‑150 (MQ=60)
                                                                                                                                  ATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTAGCGGGCAGTAAACGTGGTGCGCATCTGGCGTTAAGTCTGGAGCGG                   <  SRR19973066.1393155/150‑1 (MQ=60)
                                                                                                                                  ATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTAGCGGGCAGTAAACGTGGTGCGCATCTGGCGTTAAGTCTGGAGCGG                   >  SRR19973066.2914926/1‑150 (MQ=60)
                                                                                                                                  ATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTAGCGGGCAGTAAACGTGGTGCGCATCTGGCGTTAAGTCTGGAGCGG                   >  SRR19973066.522940/1‑150 (MQ=60)
                                                                                                                                    TCTGATCACCACACCCTCATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACTCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTAGCGGGCAGTAAACGTGGTGCGCATCTGGCGTTAAGTCTGGAGCGGCT                 <  SRR19973066.2047628/150‑1 (MQ=60)
                                                                                                                                    TCTGATCACCACACCCTACTCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTAGCGGGCAGTAAACGTGGTGCGCATCTGGCGTTAAGTCTGGAGCGGCT                 <  SRR19973066.2047597/150‑1 (MQ=60)
                                                                                                                                    TCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTAGCGGGCAGTAAACGTGGTGCGCATCTGGCGTTAAGTCTGGAGCGGCT                 <  SRR19973066.1302378/150‑1 (MQ=60)
                                                                                                                                       GATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTAGCGGGCAGTAAACGTGGTGCGCATCTGGCGTTAAGTCTGGAGagatcgga              >  SRR19973066.2898161/1‑142 (MQ=60)
                                                                                                                                        ATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTAGCGGGCAGTAAACGTGGTGCGCATCTGGCGTTAAGTCTGGAGCGGCTCGAT             <  SRR19973066.2333975/150‑1 (MQ=60)
                                                                                                                                          CACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTAGCGGGCAGTAAACGTGGTGCGCATCTGGCGTTAAGTCTGGAGCGGCTCGATGC           <  SRR19973066.1635601/150‑1 (MQ=60)
                                                                                                                                            CCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTAGCGGGCAGTAAACGTGGTGCGCATCTGGCGTTAAGTCTGGAGCGGCTCGATGCGC         >  SRR19973066.3200286/1‑150 (MQ=60)
                                                                                                                                             CACACCCTAATCACTCTCTCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTAGCGGGCAGTAAACGTGGTGCGCATCTGGCGTTAAGTCTGGAGCGGCTCGATGCGCT        <  SRR19973066.725336/150‑1 (MQ=60)
                                                                                                                                                   CTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTAGCGGGCAGTAAACGTGGTGCGCATCTGGCGTTAAGTCTGGAGCGGCTCGATGCGCTGCTCCA  <  SRR19973066.3477496/150‑1 (MQ=60)
                                                                                                                                                    |                                                                                                                                                    
GATCCCGTTGAAGGGTATTGCCGATGAACGGCGGCGGCGCGGCGAAACGGAAGTCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCGAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTAGCGGGCAGTAAACGTGGTGCGCATCTGGCGTTAAGTCTGGAGCGGCTCGATGCGCTGCTCCA  >  CP009273/1723661‑1723957

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑