Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F394 I0 R1 1342 215.0 7134094 96.2% 6862998 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,739,015 T→A 39.9% I434I (ATT→ATA)  mdtK → multidrug efflux system transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,739,0150TA39.9% 156.2 / 262.8 208I434I (ATT→ATA) mdtKmultidrug efflux system transporter
Reads supporting (aligned to +/- strand):  ref base T (70/55);  new base A (50/33);  total (120/88)
Fisher's exact test for biased strand distribution p-value = 5.69e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.45e-01

Only 100 of 211 total aligned reads displayed.

TTCCATTTTCTATATTACCTTTACGGCTTACTGGGTGCTGGGCTTGCCAAGCGGCTATATTCTGGCACTGACCGATCTGGTCGTTGAACCTATGGGGCCAGCAGGCTTCTGGATAGGCTTTATTATTGGCCTGACGTCGGCAGCCATTATGATGATGTTGCGTATGCGGTTCCTGCAACGTCTGCCGTCAGCCATCATTCTGCAACGAGCATCCCGCTAATAAAGACAAGGCGCAACCTTCACGGGTTGCGCCTGTATTTTTACGCAGGCTGGAGCGTTGCGCCAATCCCGTCT  >  CP009273/1738868‑1739161
                                                                                                                                                   |                                                                                                                                                  
ttCCATTTTCTATATTACCTTTACGGCTTACTGGGTGCTGGGCTTGCCAAGCGGCTATATTCTGGCACTGACCGATCTGGTCGTTGAACCTATGGGGCCAGCAGGCTTCTGGATAGGCTTTATTATTGGCCTGACGTCGGCAGCCAtt                                                                                                                                                    >  1:1898316/1‑148 (MQ=255)
  ccATTTTCTATATTACCTTTACGGCTTACTGGGTGCTGGGCTTGCCAAGCGGCTATATTCTGGCACTGACCGATCTGGTCGTTGAACCTATGGGGCCAGCAGGCTTCTGGATAGGCTTTATTATTGGCCTGACGTCGGCAGCCATTat                                                                                                                                                  >  2:2847162/1‑148 (MQ=255)
   cATTTTCTATATTACCTTTACGGCTTACTGGGTGCTGGGCTTGCCAAGCGGCTATATTCTGGCACTGACCGATCTGGTCGTTGAACCTATGGGGCCAGCAGGCTTCTGGATAGGCTTTATTATTGGCCTGACGTCGGCAGCCATAatg                                                                                                                                                 <  2:634579/148‑1 (MQ=255)
      tttCTATATTACCTTTACGGCTTACTGGGTGCTGGGCTTGCCAAGCGGCTATATTCTGGCACTGACCGATCTGGTCGTTGAACCTATGGGGCCAGCAGGCTTCTGGATAGGCTTTATTATTGGCCTGACGTCGGCAGCCATTatgatg                                                                                                                                              <  2:258219/148‑1 (MQ=255)
          tataTTACCTTTACGGCTTACTGGGTGCTGGGCTTGCCAAGCGGCTATATTCTGGCACTGACCGATCTGGTCGTTGAACCTATGGGGCCAGCAGGCTTCTGGATAGGCTTTATTATTGGCCTGACGTCGGCAGCCATTATGATGATGt                                                                                                                                          >  2:1546797/1‑148 (MQ=255)
           atatTACCTTTACGGCTTACTGGGTGCTGGGCTTGCCAAGCGGCTATATTCTGGCACTGACCGATCTGGTCGTTGAACCTATGGGGCCAGCAGGCTTCTGGATAGGCTTTATTATTGGCCTGACGTCGGCAGCCATTATGATGATGtt                                                                                                                                         <  1:1640830/148‑1 (MQ=255)
            tatTACCTTTACGGCTTACTGGGTGCTGGGCTTGCCAAGCGGCTATATTCTGGCACTGACCGATCTGGTCGTTGAACCTATGGGGCCAGCAGGCTTCTGGATAGGCTTTATTATTGGCCTGACGTCGGCAGCCATTATGATGATGTTg                                                                                                                                        <  2:543529/148‑1 (MQ=255)
            tatTACCTTTACGGCTTACTGGGTGCTGGGCTTGCCAAGCGGCTATATTCTGGCACTGACCGATCTGGTCGTTGAACCTATGGGGCCAGCAGGCTTCTGGATAGGCTTTATTATTGGCCTGACGTCGGCAGCCATAATGATGATGTTg                                                                                                                                        <  1:859572/148‑1 (MQ=255)
              ttACCTTTACGGCTTACTGGGTGCTGGGCTTGCCAAGCGGCTATATTCTGGCACTGACCGATCTGGTCGTTGAACCTATGGGGCCAGCAGGCTTCTGGATAGGCTTTATTATTGGCCTGACGTCGGCAGCCATTATGATGATGTTGCg                                                                                                                                      >  1:3252871/1‑148 (MQ=255)
              ttACCTTTACGGCTTACTGGGTGCTGGGCTTGCCAAGCGGCTATATTCTGGCACTGACCGATCTGGTCGTTGAACCTATGGGGCCAGCAGGCTTCTGGATAGGCTTTATTATTGGCCTGACGTCGGCAGCCATAATGATGATGTTGCg                                                                                                                                      >  2:3139166/1‑148 (MQ=255)
               tACCTTTACGGCTTACTGGGTGCTGGGCTTGCCAAGCGGCTATATTCTGGCACTGACCGATCTGGTCGTTGAACCTATGGGGCCAGCAGGCTTCTGGATAGGCTTTATTATTGGCCTGACGTCGGCAGCCATAATGATGATGTTGCGt                                                                                                                                     >  2:675501/1‑148 (MQ=255)
                aCCTTTACGGCTTACTGGGTGCTGGGCTTGCCAAGCGGCTATATTCTGGCACTGACCGATCTGGTCGTTGAACCTATGGGGCCAGCAGGCTTCTGGATAGGCTTTATTATTGGCCTGACGTCGGCAGCCATTATGATGATGTTGCGTa                                                                                                                                    >  2:882954/1‑148 (MQ=255)
                 ccTTTACGGCTTACTGGGTGCTGGGCTTGCCAAGCGGCTATATTCTGGCACTGACCGATCTGGTCGTTGAACCTATGGGGCCAGCAGGCTTCTGGATAGGCTTTATTATTGGCCTGACGTCGGCAGCCATTATGATGATGTTGCGTAt                                                                                                                                   >  1:1147254/1‑148 (MQ=255)
                      aCGGCTTACTGGGTGCTGGGCTTGCCAAGCGGCTATATTCTGGCACTGACCGATCTGGTCGTTGAACCTATGGGGCCAGCAGGCTTCTGGATAGGCTTTATTATTGGCCTGACGTCGGCAGCCATAATGATGATGTTGCGTATGCGGt                                                                                                                              <  1:811488/148‑1 (MQ=255)
                        ggCTTACTGGGTGCTGGGCTTGCCAAGCGGCTATATTCTGGCACTGACCGATCTGGTCGTTGAACCTATGGGGCCAGCAGGCTTCTGGATAGGCTTTATTATTGGCCTGACGTCGGCAGCCATTATGATGATGTTGCGTATGCGGTTc                                                                                                                            <  1:3109513/148‑1 (MQ=255)
                        ggCTTACTGGGTGCTGGGCTTGCCAAGCGGCTATATTCTGGCACTGACCGATCTGGTCGTTGAACCTATGGGGCCAGCAGGCTTCTGGATAGGCTTTATTATTGGCCTGACGTCGGCAGCCATTATGATGATGTTGCGTATGCGGTTc                                                                                                                            <  2:3494148/148‑1 (MQ=255)
                                gggTGCTGGGCTTGCCAAGCGGCTATATTCTGGCACTGACCGATCTGGTCGTTGAACCTATGGGGCCAGCAGGCTTCTGGATAGGCTTTATTATTGGCCTGACGTCGGCAGCCATAATGATGATGTTGCGTATGCGGTTCCTGCAACg                                                                                                                    >  2:610421/1‑148 (MQ=255)
                                 ggTGCTGGGCTTGCCAAGCGGCTATATTCTGGCACTGACCGATCTGGTCGTTGAACCTATGGGGCCAGCAGGCTTCTGGATAGGCTTTATTATTGGCCTGACGTCGGCAGCCATTATGATGATGTTGCGTATGCGGTTCCTGCAACGt                                                                                                                   <  1:305114/148‑1 (MQ=255)
                                 ggTGCTGGGCTTGCCAAGCGGCTATATTCTGGCACTGACCGATCTGGTCGTTGAACCTATGGGGCCAGCAGGCTTCTGGATAGGCTTTATTATTGGCCTGACGTCGGCAGCCATTATGATGATGTTGCGTATGCGGTTCCTGCAACGt                                                                                                                   <  1:1324640/148‑1 (MQ=255)
                                 ggTGCTGGGCTTGCCAAGCGGCTATATTCTGGCACTGACCGATCTGGTCGTTGAACCTATGGGGCCAGCAGGCTTCTGGATAGGCTTTATTATTGGCCTGACGTCGGCAGCCATAATGATGATGTTGCGTATGCGGTTCCTGCAACGt                                                                                                                   >  2:2323064/1‑148 (MQ=255)
                                 ggTGCTGGGCTTGCCAAGCGGCTATATTCTGGCACTGACCGATCTGGTCGTTGAACCTATGGGGCCAGCAGGCTTCTGGATAGGCTTTATTATTGGCCTGACGTCGGCAGCCATAATGATGATGTTGCGTATGCGGTTCCTGCAACGt                                                                                                                   >  1:3456152/1‑148 (MQ=255)
                                   tGCTGGGCTTGCCAAGCGGCTATATTCTGGCACTGACCGATCTGGTCGTTGAACCTATGGGGCCAGCAGGCTTCTGGATAGGCTTTATTATTGGCCTGACGTCGGCAGCCATAATGATGATGTTGCGTATGCGGTTCCTGCAACGTCt                                                                                                                 >  2:107545/1‑148 (MQ=255)
                                             gCCAAGCGGCTATATTCTGGCACTGACCGATCTGGTCGTTGAACCTATGGGGCCAGCCGGCTTCTGGCTAGGCTTTATTATTGGCCTGACGTCGGCAGCCATAATGATGATGTTGCGTATGCGGTTCCTGCAACGTCTGCCGTCAGcc                                                                                                       >  2:1842743/1‑148 (MQ=255)
                                               cAAGCGGCTATATTCTGGCACTGACCGATCTGGTCGTTGAACCTATGGGGCCAGCAGGCTTCTGGATAGGCTTTATTATTGGCCTGACGTCGGCAGCCATAATGATGATGTTGCGTATGCGGTTCCTGCAACGTCTGCCGTCAGCcat                                                                                                     <  1:991147/148‑1 (MQ=255)
                                                aaGCGGCTATATTCTGGCACTGACCGATCTGGTCGTTGAACCTATGGGGCCAGCAGGCTTCTGGATAGGCTTTATTATTGGCCTGACGTCGGCAGCCATTATGATGATGTTGCGTATGCGGTTCCTGCAACGTCTGCCGTCAGCcatc                                                                                                    >  1:882943/1‑148 (MQ=255)
                                                   cGGCTATATTCTGGCACTGACCGATCTGGTCGTTGAACCTATGGGGCCAGCAGGCTTCTGGATAGGCTTTATTATTGGCCTGACGTCGGCAGCCATTATGATGATGTTGCGTATGCGGTTCCTGCAACGTCTGCCGTCAGCCATCAtt                                                                                                 <  2:2305484/148‑1 (MQ=255)
                                                   cGGCTATATTCTGGCACTGACCGATCTGGTCGTTGAACCTATGGGGCCAGCAGGCTTCTGGATAGGCTTTATTATTGGCCTGACGTCGGCAGCCATTATGATGATGTTGCGTATGCGGTTCCTGCAACGTCTGCCGTCAGCCATCAtt                                                                                                 >  1:2088245/1‑148 (MQ=255)
                                                     gCTATATTCTGGCACTGACCGATCTGGTCGTTGAACCTATGGGGCCAGCAGGCTTCTGGATAGGCGTTATTATTGGCCTGACGTCGGCAGCCATTATGATGATGTTGCGTATGCGGTTCCTGCAACGTCTGCCGTCAGCCATCATTCt                                                                                               >  1:1742320/1‑148 (MQ=255)
                                                      cTATATTCTGGCACTGACCGATCTGGTCGTTGAACCTATGGGGCCAGCAGGCTTCTGGATAGGCTTTATTATTGGCCTGACGTCGGCAGCCATTATGATGATGTTGCGTATGCGGTTCCTGCAACGTCTGCCGTCAGCCATCATTCTg                                                                                              >  1:2707552/1‑148 (MQ=255)
                                                      cTATATTCTGGCACTGACCGATCTGGTCGTTGAACCTATGGGGCCAGCAGGCTTCTGGATAGGCTTTATTATTGGCCTGACGTCGGCAGCCATTATGATGATGTTGCGTATGCGGTTCCTGCAACGTCTGCCGTCAGCCATCATTCTg                                                                                              >  1:685267/1‑148 (MQ=255)
                                                      cTATATTCTGGCACTGACCGATCTGGTCGTTGAACCTATGGGGCCAGCAGGCTTCTGGATAGGCTTTATTATTGGCCTGACGTCGGCAGCCATTATGATGATGTTGCGTATGCGGTTCCTGCAACGTCTGCCGTCAGCCATCATTCTg                                                                                              >  2:3513574/1‑148 (MQ=255)
                                                      cTATATTCTGGCACTGACCGATCTGGTCGTTGAACCGATGGGGCCAGCAGGCTTCTGGATAGGCTTTATTATTGGCCTGACGTCGGCAGCCATTATGATGATGTTGCGTATGCGGTTCCTGCAACGTCTGCCGTCAGCCATCATTCTg                                                                                              >  1:2472588/1‑148 (MQ=255)
                                                       tataTTCTGGCACTGACCGATCTGGTCGTTGAACCTATGGGGCCAGCAGGCTTCTGGATAGGCTTTATTATTGGCCTGACGTCGGCAGCCATTATGATGATGTTGCGTATGCGGTTCCTGCAACGTCTGCCGTCAGCCATCATTCTGc                                                                                             >  1:3287704/1‑148 (MQ=255)
                                                       tataTTCTGGCACTGACCGATCTGGTCGTTGAACCTATGGGGCCAGCAGGCTTCTGGATAGGCTTTATTATTGGCCTGACGTCGGCAGCCATTATGATGATGTTGCGTATGCGGTTCCTGCAACGTCTGCCGTCAGCCATCATTCTGc                                                                                             >  2:3039982/1‑148 (MQ=255)
                                                         tatTCTGGCACTGACCGATCTGGTCGTTGAACCTATGGGGCCAGCAGGCTTCTGGATAGGCTTTATTATTGGCCTGACGTCGGCAGCCATTATGATGATGTTGCGTATGCGGTTCCTGCAACGTCTGCCGTCAGCCATCATTCTGCaa                                                                                           >  1:1902590/1‑148 (MQ=255)
                                                         tatTCTGGCACTGACCGATCTGGTCGTTGAACCTATGGGGCCAGCAGGCTTCTGGATAGGCTTTAATATTGGCCTGACGTCGGCAGCCATTATGATTATGGTGCGTATGCGGTTCCTGCAACCTCAGCCGTCAGCCATCAATCTGCaa                                                                                           >  1:593173/1‑148 (MQ=255)
                                                         tatTCTGGCACTGACCGATCTGGTCGTTGAACCTAAGGGGCCAGCAGGCATCAGGATAGGCTTTATTATTGGCCTGACCTCGGCAGCCAATAAGATTGTGTTGCGTATTCGGTTCCAGCCACGTCTGCCGTCAGCCATCATTATGCaa                                                                                           >  1:124774/1‑148 (MQ=255)
                                                          atTCTGGCACTGACCGATCTGGTCGTTGAACCTATGGGGCCAGCAGGCTTCTGGATAGGCTTTATTATTGGCCTGACGTCGGCAGCCGTTATGATGATGTTGCGTATGCGGTTCCTGCAACGTCTGCCGTCAGCCATCATTCTGCAAc                                                                                          >  2:2621169/1‑148 (MQ=255)
                                                           ttCTGGCACTGACCGATCTGGTCGTTGAACCTATGGGGCCAGCAGGCTTCTGGATAGGCTTTATTATTGGCCTGACGTCGGCAGCCATTATGATGATGTTGCGTATGCGGTTCCTGCAACGTCTGCCGTCAGCCATCATTCTGCAACg                                                                                         >  1:2357513/1‑148 (MQ=255)
                                                                gCACTGACCGATCTGGTCGTTGAACCTATGGGGCCAGCAGGCTTCTGGATAGGCTTTATTATTGGCCTGTCGTCGGCAGCCATTATGATGATGTTGCGTATGCGGTTCCTGCAACGTCTGCCGTCAGCCATCATTATGCAACGAGCAt                                                                                    >  1:3430076/1‑148 (MQ=255)
                                                                 cACTGACCGATCTGGTCGTTGAACCTATGGGGCCAGCAGGCTTCTGGATAGGCTTTATTATTGGCCTGACGTCGGCAGCCATAATGATGATGTTGCGTATGCGGTTCCTGCAACGTCTGCCGTCAGCCATCATTCTGCAACGAGCATc                                                                                   <  1:1025948/148‑1 (MQ=255)
                                                                 cACTGACCGATCTGGTCGTTGAACCTATGGGGCCAGCAGGCTTCTGGATAGGCTTTATTATTGGCCTGACGTCGGCAGCCATAATGATGATGTTGCGTATGCGGTTCCTGCAACGTCTGCCGTCAGCCATCATTCTGCAACGAGCATc                                                                                   <  1:3345828/148‑1 (MQ=255)
                                                                   cTGACCGATCTGGTCGTTGAACCTATGGGGCCAGCAGGCTTCTGGATAGGCTTTATTATTGGCCTGACGTCGGCAGCCATTATGATGATGTTGCGTATGCGGTTCCTGCAACGTCTGCCGTCAGCCATCATTCTGCAACGAGCATccc                                                                                 >  2:2860013/1‑148 (MQ=255)
                                                                   cTGACCGATCTGGTCGTTGAACCTATGGGGCCAGCAGGCTTCTGGATAGGCTTTATTATTGGCCTGACGTCGGCAGCCATTATGATGATGTTGCGTATGCGGTTCCTGCAACGTCTGCCGTCAGCCATCATTCTGCAACGAGCATccc                                                                                 >  2:2034866/1‑148 (MQ=255)
                                                                      aCCGATCTGGTCGTTGAACCTATGGGGCCAGCAGGCTTCTGGATAGGCTTTATTATTGGCCTGACGTCGGCAGCCATTATGATGATGTTGCGTATGCGGTTCCTGCAACGTCTGCCGTCAGCCATCATTCTGCAACGAGCATCCCGCt                                                                              >  2:460850/1‑148 (MQ=255)
                                                                      aCCGATCTGGTCGTTGAACCTATGGGGCCAGCAGGCTTCTGGATAGGCTTTATTATTGGCCTGACGTCGGCAGCCATAATGATGATGTTGCGTATGCGGTTCCTGCAACGTCTGCCGTCAGCCATCATTCTGCAACGAGCATCCCGCt                                                                              >  1:1192418/1‑148 (MQ=255)
                                                                      aCCGATCTGGTCGTTGAACCTATGGGGCCAGCAGGCTTCTGGATAGGCTTTATTATTGGCCTGACGTCGGCAGCCATAATGATGATGTTGCGTATGCGGTTCCTGCAACGTCTGCCGTCAGCCATCATTCTGCAACGAGCATCCCGCt                                                                              >  1:3228732/1‑148 (MQ=255)
                                                                      aCCGATCTGGTCGTTGAACCTATGGGGCCAGCAGGCTTCTGGATAGGCGTTATTATTGGCCTGACGTCGGCAGCCATAATGATGATGTTGCGTATGCGGTTCCTGCAACGTCTGCCGTCAGCCATCATTCTGCAACGAGCATCCCGCt                                                                              >  1:1643925/1‑148 (MQ=255)
                                                                      aCCGATCTGGTCGTTGAACCTATGGGGCCAGCACGCTTATGGATACGCTTTATAATTGGCCAGACGTAGGCAGCCATTATTATGATGTTGCGTATGCGGTTCCAGCAACGTCTGCCGTAAGCCAACAATCTGCAACGAGCATCCCGca                                                                              >  1:2522419/1‑147 (MQ=255)
                                                                        cGATCTGGTCGTTGAACCTATGGGGCCAGCAGGCTTCTGGATAGGCTTTATTATTGGCCTGACGTCGGCAGCCATAATGATGATGTTGCGTATGCGGTTCCTGCAACGTCTGCCGTCAGCCATCATTCTGCAACGAGCATCCCGCtaa                                                                            >  2:3177880/1‑148 (MQ=255)
                                                                           tCTGGTCGTTGAACCTATGGGGCCAGCAGGCTTCTGGATAGGCTTTATTATTGGCCTGACGTCGGCAGCCATTATGATGATGTTGCGTATGCGGTTCCTGCAACGTCTGCCGTCAGCCATCATTCTGCAACGAGCATCCCGCtaataa                                                                         <  1:354952/148‑1 (MQ=255)
                                                                              ggTCGTTGAACCTATGGGGCCAGCAGGCTTCTGGAAAGGCTTTATTATTGGCCTGACGTCGGCAGCCATAATGATGATGTTGCGTATGCGGTTCCTGCAACGTCTGCCGTCAGCCATCATTCTGCAACGAGCCTCCCGCTAATAAAGa                                                                      >  2:2298881/1‑148 (MQ=255)
                                                                                tCGTTGAACCTATGGGGCCAGCAGGCTTCTGGATAGGCTTTATTATTGGCCTGACGTCGGCAGCCATTATGATGATGTTGCGTATGCGGTTCCTGCAACGTCTGCCGTCAGCCATCATTCTGCAACGAGCATCCCGCTAATAAAGACa                                                                    <  2:3274052/148‑1 (MQ=255)
                                                                                tCGTTGAACCTATGGGGCCAGCAGGCTTCTGGATAGGCTTTATTATTGGCCTGACGTCGGCAGCCATTATGATGATGTTGCGTATGCGGTTCCTGCAACGTCTGCCGTCAGCCATCATTCTGCAACGAGCATCCCGCTAATAAAGACa                                                                    <  2:3274202/148‑1 (MQ=255)
                                                                                 cGTTGAATCTATGGGGCCATCAGGCTTATGGATATGCTTTATTATTGGACTGACGTCTTCAGCAATTATGATGATGTTGCGTATTCTGTTCCTTCAACGTCTGCCGTCAGCCATCATTCTGCATCGAGAATCCCGCTAATAAAGaaaa                                                                   <  2:2215838/148‑1 (MQ=255)
                                                                                 cGTTGAACCTATGGGGCCAGCAGGCTTCTGGATAGGCTTTATTATTGGCCTGACGTCGGCAGCCATAATGATGATGTTGCGTATGCGGTTCCTGCAACGTCTGCCGTCAGCCATCATTCTGCAACGAGCATCCCGCTAATAAAGACaa                                                                   <  2:358911/148‑1 (MQ=255)
                                                                                  gTTGAACCTATGGGGCCATCAGGATTCTGGATAGGCTTTATTATTGGCCTGACGTCGGCAGCCATAATGATGATGTTGCGTATGCGGTTCCTGCAACGTCTTCCGTCAGCCATCATTCTGCAACGAGCATCCCGCTAATAAAGACAAg                                                                  <  2:1859986/148‑1 (MQ=255)
                                                                                  gTTGAACCTATGGGGCCAGCAGGCTTCTGGATAGGCTTTATTATTGGCCTGACGTCGGCAGCCATTATGATGATGTTGCGTATGCGGTTCCTGCAACGTCTGCCGTCAGCCATCATTCTGCAACGAGCATCCCGCTAATAAAGACAAg                                                                  <  2:246851/148‑1 (MQ=255)
                                                                                  gTTGAACCTATGGGGCCAGCAGGCTTCTGGATAGGCTTTATTATTGGCCTGACGTCGGCAGCCATAATGATGATGTTGCGTATGCGGTTCCTGCAACGTCTGCCGTCAGCCATCATTCTGCAACGAGCATCCCGCTAATAAAGACAAg                                                                  <  1:95926/148‑1 (MQ=255)
                                                                                   ttGAACCTATGGGGCCAGCAGGCTTCTGGATAGGCTTTATTATTGGCCTGACGTCGGCAGCCATAATGATGATGTTGCGTATGCGGTTCCTGCAACGTCTGCCGTCAGCCATCATTCTGCAACGAGCATCCCGCTAATAAAGACAAgg                                                                 <  1:1177551/148‑1 (MQ=255)
                                                                                    tGAACCTATGGGGCCAGCAGGCTTCTGGATAGGCTTTATTATTGGCCTGACGTCGGCAGCCATTATGATGATGTTGCGTATGCGGTTCCTGCAACGTCTGCCGTCAGCCATCATTCTGCAACGAGCATCCCGCTAATAAAGACAAGgc                                                                >  2:916996/1‑148 (MQ=255)
                                                                                    tGAACCTATGGGGCCAGCAGGCTTCTGGATAGGCTTTATTATTGGCCTGACGTCGGCAGCCATAATGATGATGTTGCGTATGCGGTTCCTGCAACGTCTGCCGTCAGCCATCATTCTGCAACGAGCATCCCGCTAATAAAGACAAGgc                                                                >  1:1489328/1‑148 (MQ=255)
                                                                                    tGAACCTATGGGGCCAGCAGGCTTCTGGATAGGCTTTATTATTGGCCTGACGTCGGCAGCCATAATGATGATGTTGCGTATGCGGTTCCTGCAACGTCTGCCGTCAGCCATCATTCTGCAACGAGCATCCCGCTAATAAAGACAAGgc                                                                >  2:449060/1‑148 (MQ=255)
                                                                                      aaCCTATGGGGCCAGCAGGCTTCTGGATAGGCTTTATTATTGGCCTGACGTCGGCAGCCATTATGATGATGTTGCGTATGCGGTTCCTGCAACGTCTGCCGTCAGCCATCATTCTGCAACGAGCATCCCGCTAATAAAGACAAGgcgc                                                              >  2:2098427/1‑148 (MQ=255)
                                                                                      aaCCTATGGGGCCAGCAGGCTTCTGGATAGGCTTTATTATTGGCCTGACGTCGGCAGCCATAATGATGATGTTGCGTATGCGGTTCCTGCAACGTCTGCCGTCAGCCATCATTCTGCAACGAGCATCCCGCTAATAAAGACAAGgcgc                                                              >  2:1251142/1‑148 (MQ=255)
                                                                                      aaCCTATGGGGCCAGCAGGCTTCTGGATAGGCTTTATTATTGGCCTGACGTCGGCAGCCATAATGATGATGTTGCGTATGCGGTTCCTGCAACGTCTGCCGTCAGCCATCATTCTGCAACGAGCATCCCGCTAATAAAGACAAGgcgc                                                              >  2:1213107/1‑148 (MQ=255)
                                                                                       aCCTATGGGGCCAGCAGGCTTCTGGATAGGCTTTATTATTGGCCTGACGTCGGCAGCCATAATGATGATGTTGCGTATGCGGTTCCTGCAACGTCTGCCGTCAGCCATCATTCTGCAACGAGCATCCCGCTAATAAAGACAAGGCGCa                                                             <  2:2459389/148‑1 (MQ=255)
                                                                                         cTATGGGGCCAGCAGGCTTCTGGATAGGCTTTATTATTGGCCTGACGTCGGCAGCCATTATGATGATGTTGCGTATGCGGTTCCTGCAACGTCTGCCGTCAGCCATCATTCTGCAACGAGCATCCCGCTAATAAAGACAAGGCGCAAc                                                           <  1:665277/148‑1 (MQ=255)
                                                                                                gCCAGCAGGCTTCTGGATAGGCTTTATTATTGGCCTGACGTCGGCAGCCATTATGATGATGTTGCGTATGCGGTTCCTGCAACGTCTGCCGTCAGCCATCATTCTGCAACGAGCATCCCGCTAATAAAGACAAGGCGCAACCTTCACg                                                    <  2:1747186/148‑1 (MQ=255)
                                                                                                   agcagGCTTCTGGATAGGCTTTATTATTGGCCTGACGTCGGCAGCCATTATGATGATGTTGCGTATGCGGTTCCTGCAACGTCTGCCGTCAGCCATCATTCTGCAACGAGCATCCCGCTAATAAAGACAAGGCGCAACCTTCACGGGt                                                 >  1:1890733/1‑148 (MQ=255)
                                                                                                   agcagGCTTCTGGATAGGCTTTATTATTGGCCTGACGTCGGCAGCCATAATGATGATGTTGCGTATGCGGTTCCTGCAACGTCTGCCGTCAGCCATCATTCTGCAACGAGCATCCCGCTAATAAAGACAAGGCGCAACCTTCACGGGt                                                 <  1:1431258/148‑1 (MQ=255)
                                                                                                             tGGATAGGCTTTATTATTGGCCTGACGTCGGCAGCCATTATGATGATGTTGCGTATGCGGTTCCTGCAACGTCTGCCGTCAGCCATCATTCTGCAACGAGCATCCCGCTAATAAAGACAAGGCGCAACCTTCACGGGTTGCGCCTGTa                                       <  1:436070/148‑1 (MQ=255)
                                                                                                                aTAGGCTTTATTATTGGCCTGACGTCGGCAGCCATAATGATGATGTTGCGTATGCGGTTCCTGCAACGTCTGCCGTCAGCCATCATTCTGCAACGAGCATCCCGCTAATAAAGACAAGGCGCAACCTTCACGGGTTGCGCCTGTAttt                                    <  2:2973081/148‑1 (MQ=255)
                                                                                                                aTAGGCTTTATTATTGGCCTGACGTCGGCAGCCATAATGATGATGTTGCGTATGCGGTTCCTGCAACGTCTGCCGTCAGCCATCATTCTGCAACGAGCATCCCGCTAATAAAGACAAGGCGCAACCTTCACGGGTTGCGCCTGTAttt                                    <  2:1300434/148‑1 (MQ=255)
                                                                                                                  aGGCTTTATTATTGGCCTGACGTCGGCAGCCATTATGATGATGTTGCGTATGCGGTTCCTGCAACGTCTGCCGTCAGCCATCATTCTGCAACGAGCATCCCGCTAATAAAGACAAGGCGCAACCTTCACGGGTTGCGCCTGTAttttt                                  >  1:1603331/1‑148 (MQ=255)
                                                                                                                   ggCTTTATTATTGGCCTGACGTCGGCAGCCATAATGATGATGTTGCGTATGCGGTTCCTGCAACGTCTGCCGTCAGCCATCATTCTGCAACGAGCATCCCGCTAATAAAGACAAGGCGCAACCTTCACGGGTTGCGCCTGTATTTTTa                                 >  2:1371562/1‑148 (MQ=255)
                                                                                                                    gCTTTATTATTGGCCTGACGTCGGCAGCCATAATGATGATGTTGCGTATGCGGTTCCTGCAACGTCTGCCGTCAGCCATCATTCTGCAACGAGCATCCCGCTAATAAAGACAAGGCGCAACCTTCACGGGTTGCGCCTGTATTTTTAc                                >  2:506103/1‑148 (MQ=255)
                                                                                                                    gCTTTATTATTGGCCTGACGTCGGCAGCCATAATGATGATGTTGCGTATGCGGTTCCTGCAACGTCTGCCGTCAGCCATCATTCTGCAACGAGCATCCCGCTAATAAAGACAAGGCGCAACCTTCACGGGTTGCGCCTGTATTTTTAc                                >  1:372104/1‑148 (MQ=255)
                                                                                                                       ttattaTTGGCCTGACGTCGGCAGCCATTATGATGATGTTGCGTATGCGGTTCCTGCAACGTCTGCCGTCAGCCATCATTCTGCAACGAGCATCCCGCTAATAAAGACAAGGCGCAACCTTCACGGGTTGCGCCTGTATTTTTACGCa                             <  1:2438485/148‑1 (MQ=255)
                                                                                                                       ttattaTGGGCCTGACGTCGGCAGCCATTATGATGATGTTGCGTATGCGGTTCCTGCAACGTCTGCCGTCAGCCATCATTCTGCAACGAGCATCCCGCTAATAAAGACAAGGCGCAACCTTCACGGGTTGCGCCTGTATTTTTACGCa                             <  1:2772012/148‑1 (MQ=255)
                                                                                                                        tattatTGGCCTGACGTCGGCAGCCATAATGATGATGTTGCGTATGCGGTTCCTGCAACGTCTGCCGTCAGCCATCATTCTGCAACGAGCATCCCGCTAATAAAGACAAGGCGCAACCTTCACGGGTTGCGCCTGTATTTTTACGCAg                            <  2:132737/148‑1 (MQ=255)
                                                                                                                          ttattGGCCTGACGTCGGCAGCCATTATGATGATGTTGCGTATGCGGTTCCTGCAACGTCTGCCGTCAGCCATCATTCTGCAACGAGCATCCCGCTAATAAAGACAAGGCGCAACCTTCACGGGTTGCGCCTGTATTTTTACGCAGGc                          <  1:1079961/148‑1 (MQ=255)
                                                                                                                           tattGGCCTGACGTCGGCAGCCATTATGATGATGTTGCGTATGCGGTTCCTGCAACGTCTGCCGTCAGCCATCATTCTGCAACGAGCATCCCGCTAATAAATACAAGGCGCAACCTTCACGGGTTGCGCCTGTATTTTTACGCAGGCt                         >  1:3406844/1‑148 (MQ=255)
                                                                                                                           tattGGCCTGACGTCGGCAGCCATTATGATGATGTTGCGTATGCGGTTCCTGCAACGTCTGCCGTCAGCCATCATTCTGCAACGAGCATCCCGCTAATAAAGACAAGGCGCAACCTTCACGGGTTGCGCCTGTATTTTTACGCAGGCt                         <  2:2408070/148‑1 (MQ=255)
                                                                                                                           tattGGCCTGACGTCGGCAGCCATTATGATGATGTTGCGTATGCGGTTCCTGCAACGTCTGCCGTCAGCCATCATTCTGCAACGAGCATCCCGCTAATAAAGACAAGGCGCAACCTTCACGGGTTGCGCCTGTATTTTTACGCAGGCt                         >  2:3318760/1‑148 (MQ=255)
                                                                                                                             ttGGCCTGACGTCGGCAGCCATTATGATGATGTTGCGTATGCGGTTCCTGCAACGTCTGCCGTCAGCCATCATTCTGCAACGAGCATCCCGCTAATAAAGACAAGGCGCAACCTTCACGGGTTGCGCCTGTATTTTTACGCAGGCTgg                       <  1:263272/148‑1 (MQ=255)
                                                                                                                              tGGCCTGACGTCGGCAGCCATTATGATGATGTTGCGTATGCGGTTCCTGCAACGTCTGCCGTCAGCCATCATTCTGCAACGAGCATCCCGCTAATAAAGACAAGGCGCAACCTTCACGGGTTGCGCCTGTATTTTTACGCAGGCTGGa                      <  1:2847183/148‑1 (MQ=255)
                                                                                                                               ggCCTGACGTCGGCAGCCATTATGATGATGTTGCGTATGCGGTTCCTGCAACGTCTGCCGTCAGCCATCATTCTGCAACGAGCATCCCGCTAATAAAGACAAGGCGCAACCTTCACGGGTTGCGCCTGTATTTTTACGCAGGCTGGAg                     <  2:2318758/148‑1 (MQ=255)
                                                                                                                                  cTGACGTCGGCAGCCATAATGATGATGTTGCGTATGCGGTTCCTGCAACGTCTGCCGTCAGCCATCATTCTGCAACGAGCATCCCGCTAATAAAGACAAGGCGCAACCTTCACGGGTTGCGCCTGTATTTTTACGCAGGCTGGAGCGt                  <  1:2416033/148‑1 (MQ=255)
                                                                                                                                   tGACGTCGGCAGCCATAATGATGATGTTGCGTATGCGGTTCCTGCAACGTCTGCCGTCAGCCATCATTCTGCAACGAGCATCCCGCTAATAAAGACAAGGCGCAACCTTCACGGGTTGCGCCTGTATTTTTACGCAGGCTGGAGCGtt                 >  2:2280622/1‑148 (MQ=255)
                                                                                                                                    gACGTCGGCAGCCATTATGATGATGTTGCGTATGCGGTTCCTGCAACGTCTGCCGTCAGCCATCATTCTGCAACGAGCATCCCGCTAATAAAGACAAGGCGCAACCTTCACGGGTTGCGCCTGTATTTTTACGCAGGCTGGAGCGTTg                >  2:1413422/1‑148 (MQ=255)
                                                                                                                                       gTCGGCAGCCATTATGATGATGTTGCGTATGCGGTTCCTGCAACGTCTGCCGTCAGCCATCATTCTGCAACGAGCATCCCGCTATTAAAGACAAGGCGCAACCTTCACGGGTTGCGCCTGTATTTTTACGCAGGCTGGAGCGTTgcgc             <  2:864085/148‑1 (MQ=255)
                                                                                                                                        tCGGCAGCCATAATGATGATGTTGCGTATGCGGTTCCTGCACCGTCTGCCGTCAGCCATCATTCTGCAACGAGCATCCCGCTAATAAAGACAAGGCGCAACCTTCACGGGTTGCGCCTGTATTTTTACGCAGGCTGGAGCGTTGCGcc            >  1:1894828/1‑148 (MQ=255)
                                                                                                                                        tCGGCAGCCATAATGATGATGTTGCGTATGCGGTTCCTGCAACGTCTGCCGTCAGCCATCATTCTGCAACGAGCATCCCGCTAATAAAGACAAGGCGCAACCTTCACGGGTTGCGCCTGTATTTTTACGCAGGCTGGAGCGTTGCGcc            >  2:2277825/1‑148 (MQ=255)
                                                                                                                                          ggCAGCCATAATGATGATGTTGCGTATGCGGTTCCTGCAACGTCTGCCGTCAGCCATCATTCTGCAACGAGCATCCCGCTAATAAAGACAAGGCGCAACCTTCACGGGTTGCGCCTGTATTTTTACGCAGGCTGGAGCGTTGCGCCaa          <  1:1014170/148‑1 (MQ=255)
                                                                                                                                            cAGCCATTATGATGATGTTGCGTATGCGGTTCCTGCAACGTCTGCCGTCAGCCATCATTCTGCAACGAGCATCCCGCTAATAAAGACAAGGCGCAACCTTCACGGGTTGCGCCTGTATTTTTACGCAGGCTGGAGCGTTGCGCCAATc        <  2:1898353/148‑1 (MQ=255)
                                                                                                                                             aGCCATTATGATGATGTTGCGTATGCGGTTCCTGCAACGTCTGCCGTCAGCCATCATTCTGCAACGAGCATCCCGCTAATAAAGACAAGGCGCAACCTTCACGGGTTGCGCCTGTATTTTTACGCAGGCTGGAGCGTTGCGCCAATcc       >  1:1837539/1‑148 (MQ=255)
                                                                                                                                               ccATAATGATGATGTTGCGTATGCGGTTCCTGCAACGTCTGCCGTCAGCCATCATTCTGCAACGAGCATCCCGCTAATAAAGACAAGGCGCAACCTTCACGGGTTGCGCCTGTATTTTTACGCAGGCTGGAGCGTTGCGCCAATCCCg     >  1:2961568/1‑148 (MQ=255)
                                                                                                                                                 aTTATGATGATGTTGCGTATGCGGTTCCTGCAACGTCTGCCGTCAGCCATCATTCTGCAACGAGCATCCCGCTAATAAAGACAAGGCGCAACCTTCACGGGTTGCGCCTGTATTTTTACGCAGGCTGGAGCGTTGCGCCAATCCCGTc   <  1:2207744/148‑1 (MQ=255)
                                                                                                                                                 aTTATGATGATGTTGCGTATGCGGTTCCTGCAACGTCTGCCGTCAGCCATCATTCTGCAACGAGCATCCCGCTAATAAAGACAAGGCGCAACCTTCACGGGTTGCGCCTGTATTTTTACGCAGGCTGGAGCGTTGCGCCAATCCCGTc   <  1:318275/148‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
TTCCATTTTCTATATTACCTTTACGGCTTACTGGGTGCTGGGCTTGCCAAGCGGCTATATTCTGGCACTGACCGATCTGGTCGTTGAACCTATGGGGCCAGCAGGCTTCTGGATAGGCTTTATTATTGGCCTGACGTCGGCAGCCATTATGATGATGTTGCGTATGCGGTTCCTGCAACGTCTGCCGTCAGCCATCATTCTGCAACGAGCATCCCGCTAATAAAGACAAGGCGCAACCTTCACGGGTTGCGCCTGTATTTTTACGCAGGCTGGAGCGTTGCGCCAATCCCGTCT  >  CP009273/1738868‑1739161

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A