Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F394 I0 R1 1342 215.0 7134094 96.2% 6862998 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,149,603 A→T 100% E287V (GAA→GTA)  mdtB → multidrug efflux system, subunit B

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,149,6030AT100.0% 592.7 / NA 182E287V (GAA→GTA) mdtBmultidrug efflux system, subunit B
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (82/100);  total (82/100)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 182 total aligned reads displayed.

TCCGCGAACGACCAGATGCAATCCGCCGAAGAGTATCGCCAGCTAATCATCGCCTACCAGAACGGCGCGCCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGAACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGACAGCATTCGGCAGATGCTGCCACAGCTCACTGAGAGTCTGCCGAAATCGGTGAAGGTGACAGTGCTTTCCGATCGCACCACCAA  >  CP009273/2149458‑2149747
                                                                                                                                                 |                                                                                                                                                
    cgAACGACCAGATGCAATCCGCCGAAGAGTATCGCCAGCTAATCATCGCCTACCAGAACGGCGCGCCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGc                                                                                                                                            >  1:3473892/1‑148 (MQ=255)
      aaCGACCAGATGCAATCCGCCGAAGAGTATCGCCAGCTAATCATCGCCTACCAGAACGGCGCGCCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCa                                                                                                                                          <  2:3028818/148‑1 (MQ=255)
       aCGACCAGATGCAATCCGCCGAAGAGTATCGCCAGCTAATCATCGCCTACCAGAACGGCGCGCCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCAt                                                                                                                                         >  1:24072/1‑148 (MQ=255)
       aCGACCAGATGCAATCCGCCGAAGAGTATCGCCAGCTAATCATCGCCTACCAGAACGGCGCGCCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCAt                                                                                                                                         >  1:1424248/1‑148 (MQ=255)
        cGACCAGATGCAATCCGCCGAAGAGTATCGCCAGCTAATCATCGCCTACCAGAACGGCGCGCCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCAtt                                                                                                                                        >  2:1903352/1‑148 (MQ=255)
        cGACCAGATGCAATCCGCCGAAGAGTATCGCCAGCTAATCATCGCCTACCAGAACGGCGCGCCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCAtt                                                                                                                                        >  1:2410459/1‑148 (MQ=255)
         gACCAGATGCAATCCGCCGAAGAGTATCGCCAGCTACTCATCGCCTACCAGAACGGCGCGCCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATtg                                                                                                                                       <  2:1790607/148‑1 (MQ=255)
          aCCAGATGCAATCCGCCGAAGAGTATCGCCAGCTAATCATCGCCTACCAGAACGGCGCGCCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATtgt                                                                                                                                      >  2:66830/1‑148 (MQ=255)
              gATGCAATCCGCCGAAGAGTATCGCCAGCTAATCATCGCCTACCAGAACGGCGCGCCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGtgatg                                                                                                                                  <  2:1405532/148‑1 (MQ=255)
              gATGCAATCCGCCGAAGAGTATCGCCAGCTAATCATCGCCTACCAGAACGGCGCGCCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGtgatg                                                                                                                                  >  2:138907/1‑148 (MQ=255)
                tGCAATCCGCCGAAGAGTATCGCCAGCTAATCATCGCCTACCAGAACGGCGCGCCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGaa                                                                                                                                <  1:2982414/148‑1 (MQ=255)
                tGCAATCCGCCGAAGAGTATCGCCAGCTAATCATCGCCTACCAGAACGGCGCGCCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGaa                                                                                                                                >  2:1227164/1‑148 (MQ=255)
                 gCACTCCGCCGAAGAGTATCGCCAGCTACTCATCGCCTACCAGAACGGCGCGCCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAAt                                                                                                                               <  1:2789530/148‑1 (MQ=255)
                 gCAATCCGCCGAAGAGTATCGCCAGCTAATCATCGCCTACCAGAACGGCGCGCCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAAt                                                                                                                               <  2:1949214/148‑1 (MQ=255)
                 gCAATCCGCCGAAGAGTATCGCCAGCTAATCATCGCCTACCAGAACGGCGCGCCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAAt                                                                                                                               <  2:459107/148‑1 (MQ=255)
                  cAATCCGCCGAAGAGTATCGCCAGCTAATCATCGCCTACCAGAACGGCGCGCCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATg                                                                                                                              <  1:1591591/148‑1 (MQ=255)
                   aaTCCGCCGAAGAGTATCGCCAGCTAATCATCGCCTACCAGAACGGCGCGCCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGt                                                                                                                             <  2:1534905/148‑1 (MQ=255)
                    aTCCGCCGAAGAGTATCGCCAGCTAATCATCGCCTACCAGAACGGCGCGCCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGtt                                                                                                                            >  1:3109414/1‑148 (MQ=255)
                     tCCGCCGAAGAGTATCGCCAGCTAATCATCGCCTACCAGAACGGCGCGCCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTc                                                                                                                           <  1:3473152/148‑1 (MQ=255)
                      ccgccgAAGAGTATCGCCAGCTAATCATCGCCTACCAGAACGGCGCGCCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCa                                                                                                                          >  1:129943/1‑148 (MQ=255)
                       cgccgAAGAGTATCGCCAGCTAATCATCGCCTACCAGAACGGCGCGCCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAg                                                                                                                         <  1:2549425/148‑1 (MQ=255)
                            aaGAGTATCGCCAGCTAATCATCGCCTACCAGAACGGCGCGCCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCa                                                                                                                    >  2:1104860/1‑148 (MQ=255)
                            aaGAGTATCGCCAGCTAATCATCGCCTACCAGAACGGCGCGCCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCa                                                                                                                    >  2:227089/1‑148 (MQ=255)
                            aaGAGTATCGCCAGCTAATCATCGCCTACCAGAACGGCGCGCCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCa                                                                                                                    >  2:226999/1‑148 (MQ=255)
                             agagTATCGCCAGCTAATCATCGCCTACCAGAACGGCGCGCCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAg                                                                                                                   >  2:736444/1‑148 (MQ=255)
                              gagTATCGCCAGCTAATCATCGCCTACCAGAACGGCGCGCCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCACGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGc                                                                                                                  <  1:1513481/148‑1 (MQ=255)
                              gagTATCGCCAGCTAATCATCGCCTACCAGAACGGCGCGCCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCACGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGc                                                                                                                  <  1:1513460/148‑1 (MQ=255)
                                 tATCGCCAGCTAATCATCGCCTACCAGAACGGCGCGCCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCg                                                                                                               >  1:802366/1‑148 (MQ=255)
                                  aacGCCAGCTAATCATCGCCTACCAGAACGGCTCGCCAATTCGTCTGGGCGATGTCGCAACTGAAGATCAAGGTGCAGAAAACAGCTCGCTCGGCGCGTGGGCTAACAAAGTACAGGCCATTGTGATGACTGTTCAGCGCCAGCCAgg                                                                                                              >  2:323492/3‑148 (MQ=255)
                                    cGCCAGCTAATCATCGCCTACCAGAACGGCGCGCCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTg                                                                                                            <  1:3435374/148‑1 (MQ=255)
                                     gCCAGCTAATCATCGCCTACCAGAACGGCGCGCCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGc                                                                                                           <  1:3370283/148‑1 (MQ=255)
                                       cAGCTAATCATCGCCTACCAGAACGGCGCGCCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTa                                                                                                         <  1:354738/148‑1 (MQ=255)
                                       cAGCTAATCATCGCCGACCAGAACGGCGCGCCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTa                                                                                                         <  1:353919/148‑1 (MQ=255)
                                        aGCTAATCATCGCCTACCAGAACGGCGCGCCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTaa                                                                                                        >  1:3070006/1‑148 (MQ=255)
                                         gCTAATCATCGCCTACCAGAACGGCGCGCCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAAc                                                                                                       >  2:589504/1‑148 (MQ=255)
                                         gCTAATCATCGCCTACCAGAACGGCGCGCCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGCGCTAAc                                                                                                       <  2:1431156/148‑1 (MQ=255)
                                             atcatcGCCTACCAGAACGGCGCGCCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTa                                                                                                   >  2:2122142/1‑148 (MQ=255)
                                              tcatcGCCTACCAGAACGGCGCGCCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTAt                                                                                                  >  2:218735/1‑148 (MQ=255)
                                               catcGCCTACCAGAACGGCGCGCCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTAtc                                                                                                 >  1:2191091/1‑148 (MQ=255)
                                                   gCCTACCAGAACGGCGCGCCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGACAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCa                                                                                             <  1:970888/148‑1 (MQ=255)
                                                   gCCTACCAGAACGGCGCGCCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCa                                                                                             <  1:971659/148‑1 (MQ=255)
                                                        ccAGAACGGCGCGCCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCAccgcc                                                                                        >  1:747358/1‑148 (MQ=255)
                                                        ccAGAACGGCGCGCCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCAccgcc                                                                                        >  2:186858/1‑148 (MQ=255)
                                                         cAGAACGGCGCGCCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCAccgccg                                                                                       >  1:18293/1‑148 (MQ=255)
                                                           gatcGGCGCGCCAAGTCGTCTGGGCGTTGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGAc                                                                                     <  2:2680815/145‑1 (MQ=255)
                                                           gAACGGCGCGCCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGAc                                                                                     >  1:3404960/1‑148 (MQ=255)
                                                                 cgcgcCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGATCAGCGCCAGCCCGGTGCTAACAGTATCTCCACCGCCGACAGCagt                                                                               >  2:1841362/1‑146 (MQ=255)
                                                                    gcCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGACAGCATTCgg                                                                            <  2:1605142/148‑1 (MQ=255)
                                                                     ccAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGACAGCATTCGGc                                                                           <  1:1634821/148‑1 (MQ=255)
                                                                      cAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGACAGCATTCGGCa                                                                          <  2:2413221/148‑1 (MQ=255)
                                                                        aTTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGACAGCATTCGGCAGa                                                                        >  1:3196140/1‑148 (MQ=255)
                                                                           cGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGACAGCATTCGGCAGAtgc                                                                     <  2:3463429/148‑1 (MQ=255)
                                                                             tCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGACAGCATTCGGCAGAtgctg                                                                   <  2:2285951/148‑1 (MQ=255)
                                                                             tCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGACAGCATTCGGCAGAtgctg                                                                   >  2:2773844/1‑148 (MQ=255)
                                                                              cTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGACAGCATTCGGCAGAtgctgc                                                                  <  2:3303212/148‑1 (MQ=255)
                                                                              cTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGACAGCATTCGGCAGAtgctgc                                                                  <  2:3473892/148‑1 (MQ=255)
                                                                              cTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGACAGCATTCGGCAGAtgctgc                                                                  <  1:1641474/148‑1 (MQ=255)
                                                                                gggCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGACAGCATTCGGCAGATGCTGCca                                                                >  1:2833828/1‑148 (MQ=255)
                                                                                gggCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGACAGCATTCGGCAGATGCTGCca                                                                >  2:3086100/1‑148 (MQ=255)
                                                                                gggCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGACAGCATTCGGCAGATGCTGCca                                                                >  1:1530579/1‑148 (MQ=255)
                                                                                 ggCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGACAGCATTCGGCAGATGCTGCcac                                                               >  1:3167874/1‑148 (MQ=255)
                                                                                 ggCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGACAGCATTCGGCAGATGCTGCcac                                                               >  1:1615290/1‑148 (MQ=255)
                                                                                        tCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGACAGCATTCGGCAGATGCTGCCACAGCTCAc                                                        <  1:3341193/148‑1 (MQ=255)
                                                                                           cAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGACAGCATTCGGCAGATGCTGCCACAGCTCACTga                                                     >  2:1698618/1‑148 (MQ=255)
                                                                                            aaCTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGACAGCATTCGGCAGATGCTGCCACAGCTCACTgag                                                    <  1:3206530/148‑1 (MQ=255)
                                                                                               tGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGACAGCATTCGGCAGATGCTGCCACAGCTCACTGAGAGt                                                 <  1:1171709/148‑1 (MQ=255)
                                                                                                 tAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGACAGCATTCGGCAGATGCTGCCACAGCTCACTGAGAGTCt                                               >  1:889404/1‑148 (MQ=255)
                                                                                                  agagCAAGGTGCAGAAAACAGCTGGCTCGGCGCTTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGACAGCATTCGGCAGATGCTGCCACAGCTCACTGAGAGTCTg                                              <  2:1538291/148‑1 (MQ=255)
                                                                                                  agagCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGACAGCATTCGGCAGATGCTGCCACAGCTCACTGAGAGTCTg                                              <  1:1533140/148‑1 (MQ=255)
                                                                                                   gagCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGACAGCATTCGGCAGATGCTGCCACAGCTCACTGAGAGTCTGc                                             >  1:3135410/1‑148 (MQ=255)
                                                                                                       aaGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGACAGCATTCGGCAGATGCTGCCACAGCTCACTGAGAGTCTGCCGaa                                         <  1:269656/148‑1 (MQ=255)
                                                                                                       aaGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGACAGCATTCGGCAGATGCTGCCACAGCTCACTGAGAGTCTGCCGaa                                         <  1:2093989/148‑1 (MQ=255)
                                                                                                       aaGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGACAGCATTCGGCAGATGCTGCCACAGCTCACTGAGAGTCTGCCGaa                                         <  1:1949547/148‑1 (MQ=255)
                                                                                                         ggTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGACAGCATTCGGCAGATGCTGCCACAGCTCACTGAGAGTCTGCCGAAAt                                       <  2:1052423/148‑1 (MQ=255)
                                                                                                         ggTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGACAGCATTCGGCAGATGCTGCCACAGCTCACTGAGAGTCTGCCGAAAt                                       >  2:2631303/1‑148 (MQ=255)
                                                                                                          gTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGACAGCATTCGGCAGATGCTGCCACAGCTCACTGAGAGTCTGCCGAAATc                                      <  1:2607058/148‑1 (MQ=255)
                                                                                                          gTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGACAGCATTCGGCAGATGCTGCCACAGCTCACTGAGAGTCTGCCGAAATc                                      <  2:2025727/148‑1 (MQ=255)
                                                                                                            gCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGACAGCATTCGGCAGATGCTGCCACAGCTCACTGAGAGTCTGCCGAAATCgg                                    <  1:913045/148‑1 (MQ=255)
                                                                                                               gAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGACAGCATTCGGCAGATGCTGCCACAGCTCACTGAGAGTCTGCCGAAATCGGTGa                                 <  2:2404049/148‑1 (MQ=255)
                                                                                                                 aaaCAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGACAGCATTCGGCAGATGCTGCCACAGCTCACTGAGAGTCTGCCGAAATCGGTGAAg                               >  2:2491526/1‑148 (MQ=255)
                                                                                                                   aCAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGACAGCATTCGGCAGATGCTGCCACAGCTCACTGAGAGTCTGCCGAAATCGGTGAAGGt                             >  1:1394822/1‑148 (MQ=255)
                                                                                                                   aCAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGACAGCATTCGGCAGATGCTGCCACAGCTCACTGAGAGTCTGCCGAAATCGGTGAAGGt                             <  1:2267704/148‑1 (MQ=255)
                                                                                                                          gCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGACAGCATTCGGCAGATGCTGCCACAGCTCACTGAGAGTCTGCCGAAATCGGTGAAGGTGACAGTg                      >  2:579969/1‑148 (MQ=255)
                                                                                                                            tCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGACAGCATTCGGCAGATGCTGCCACAGCTCACTGAGAGTCTGCCGAAATCGGTGAAGGTGACAGTGCt                    <  2:3282707/148‑1 (MQ=255)
                                                                                                                            tCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGACAGCATTCGGCAGATGCTGCCACAGCTCACTGAGAGTCTGCCGAAATCGGTGAAGGTGACAGTGCt                    <  2:3227685/148‑1 (MQ=255)
                                                                                                                             cGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGACAGCATTCGGCAGATGCTGCCACAGCTCACTGAGAGTCTGCCGAAATCGGTGAAGGTGACAGTGCtt                   <  2:747358/148‑1 (MQ=255)
                                                                                                                              ggCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGACAGCATTCGGCAGATGCTGCCACAGCTCACTGAGAGTCTGCCGAAATCGGTGAAGGTGACAGTGCttt                  <  1:1593002/148‑1 (MQ=255)
                                                                                                                               gcgcGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGACAGCATTCGGCAGATGCTGCCACAGCTCACTGAGAGTCTGCCGAAATCGGTGAAGGTGACAGTGCTTTc                 <  1:2620514/148‑1 (MQ=255)
                                                                                                                               gcgcGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGACAGCATTCGGCAGATGCTGCCACAGCTCACTGAGAGTCTGCCGAAATCGGTGAAGGTGACAGTGCTTTc                 >  1:1944812/1‑148 (MQ=255)
                                                                                                                                  cgTGGGCGAACAAAGTACATGCGATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTATCATTATCTCCACCGCCGACAGCATTCGGCAGATGCTGCCACAGCTCACTGAGAGTCTGCCGATATCGGTGAAGGTGACAGTGCTTTCCGa              <  1:2117672/148‑1 (MQ=255)
                                                                                                                                   gTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGACAGCATTCGGCAGATGCTGCCACAGCTCACTGAGAGTCTGCCGAAATCGGTGAAGGTGACAGTGCTTTCCGAt             <  1:1903352/148‑1 (MQ=255)
                                                                                                                                     gggCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAGCATTATCTCCACCGCCGACAGCATTCGGCAGATGCTGCCACAGCTCACTGAGAGTCTGCCGAAATCGGTGAAGGTGACAGTGCTTTCCGATCg           >  2:2766541/1‑148 (MQ=255)
                                                                                                                                       gCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGACAGCATTCGGCAGATGCTGCCACAGCTCACTGAGAGTCTGCCGAAATCGGTGAAGGTGACAGTGCTTTCCGATCGca         >  1:1048460/1‑148 (MQ=255)
                                                                                                                                        cGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGACAGCATTCGGCAGATGCTGCCACAGCTCACTGAGAGTCTGCCGAAATCGGTGAAGGTGACAGTGCTTTCCGATCGcac        <  2:3027883/148‑1 (MQ=255)
                                                                                                                                           aCAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGACAGCATTCGGCAGATGCTGCCACAGCTCACTGAGAGTCTGCCGAAATCGGTGAAGGTGACAGTGCTTTCCGATCGcaccac     >  1:2891611/1‑148 (MQ=255)
                                                                                                                                           aCAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGACAGCATTCGGCAGATGCTGCCACAGCTCACTGAGAGTCTGCCGAAATCGGTGAAGGTGACAGTGCTTTCCGATCGcaccac     >  1:579640/1‑148 (MQ=255)
                                                                                                                                             aaaGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGACAGCATTCGGCAGATGCTGCCACAGCTCACTGAGAGTCTGCCGAAATCGGTGAAGGTGACGGTGCTTTCCGATCGCAccacca   >  2:2706044/1‑148 (MQ=255)
                                                                                                                                             aaaGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGACAGCATTCGGCAGATGCTGCCACAGCTCACTGAGAGTCTGCCGAAATCGGTGAAGGTGACAGTGCTTTCCGATCGCAccacca   <  2:258989/148‑1 (MQ=255)
                                                                                                                                             aaaGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGACAGCATTCGGCAGATGCTGCCACAGCTCACTGAGAGTCTGCCGAAATCGGTGAAGGTGACAGTGCTTTCCGATCGCAccacca   <  2:24072/148‑1 (MQ=255)
                                                                                                                                             aaaGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGACAGCATTCGGCAGATGCTGCCACAGCTCACTGAGAGTCTGCCGAAATCGGTGAAGGTGACAGTGCTTTCCGATCGCAccacca   <  1:71605/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
TCCGCGAACGACCAGATGCAATCCGCCGAAGAGTATCGCCAGCTAATCATCGCCTACCAGAACGGCGCGCCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGAACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGACAGCATTCGGCAGATGCTGCCACAGCTCACTGAGAGTCTGCCGAAATCGGTGAAGGTGACAGTGCTTTCCGATCGCACCACCAA  >  CP009273/2149458‑2149747

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CTTTCCGCGAACGACCAGATGCAATCCGCCGAAGAGTATCGCCAGCTAATCATCGCCTACCAGAACGGCGCGCCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGAACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGACAGCATTCGGCAGATGCTGCCACAGCTCACTGAGAGTCTGCCGAAATCGGTGAAGGTGACAGTGCTTTCCGATCGCACCACCAATA  >  CP009273/2149455‑2149749
                                                                                                                                                    |                                                                                                                                                  
CTTTCCGCGAACGACCAGATGCAATCCGCCGAAGAGTATCGCCAGCTAATCATCGCCTACCAGAACGGCGCGCCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTA                                                                                                                                                   <  SRR19973066.482139/150‑1 (MQ=60)
CTTTCCGCGAACGACCAGATGCAATCCGCCGAAGAGTATCGCCAGCTAATCATCGCCTACCAGAACGGCGCGCCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTA                                                                                                                                                   >  SRR19973066.696875/1‑150 (MQ=60)
   TCCGCGAACGACCAGATGCAATCCGCCGAAGAGTATCGCCAGCTAATCATCGCCTACCAGAACGGCGCGCCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAG                                                                                                                                                <  SRR19973066.1995639/150‑1 (MQ=60)
     CGCGAACGACCAGATGCAATCCGCCGAAGAGTATCGCCAGCTAATCATCGCCTACCAGAACGGCGCGCCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGC                                                                                                                                              >  SRR19973066.3496797/1‑150 (MQ=60)
     CGCGAACGACCAGATGCAATCCGCCGAAGAGTATCGCCAGCTAATCATCGCCTACCAGAACGGCGCGCCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGC                                                                                                                                              >  SRR19973066.3497088/1‑150 (MQ=60)
        GAACGACCAGATGCAATCCGCCGAAGAGTATCGCCAGCTAATCATCGCCTACCAGAACGGCGCGCCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCAT                                                                                                                                           >  SRR19973066.24423/1‑150 (MQ=60)
        GAACGACCAGATGCAATCCGCCGAAGAGTATCGCCAGCTAATCATCGCCTACCAGAACGGCGCGCCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCAT                                                                                                                                           >  SRR19973066.3218340/1‑150 (MQ=60)
        GAACGACCAGATGCAATCCGCCGAAGAGTATCGCCAGCTAATCATCGCCTACCAGAACGGCGCGCCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCAT                                                                                                                                           >  SRR19973066.1434430/1‑150 (MQ=60)
         AACGACCAGATGCAATCCGCCGAAGAGTATCGCCAGCTAATCATCGCCTACCAGAACGGCGCGCCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATT                                                                                                                                          >  SRR19973066.2426780/1‑150 (MQ=60)
         AACGACCAGATGCAATCCGCCGAAGAGTATCGCCAGCTAATCATCGCCTACCAGAACGGCGCGCCAAGTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATT                                                                                                                                          <  SRR19973066.3002822/150‑1 (MQ=60)
                 GATGCAATCCGCCGAAGAGTATCGCCAGCTAATCATCGCCTACCAGAACGGCGCGCCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAA                                                                                                                                  >  SRR19973066.676537/1‑150 (MQ=60)
                   TGCAATCCGCCGAAGAGTATCGCCAGCTAATCATCGCCTACCAGAACGGCGCGCCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATG                                                                                                                                <  SRR19973066.3002113/150‑1 (MQ=60)
                    GCACTCCGCCGAAGAGTATCGCCAGCTACTCATCGCCTACCAGAACGGCGCGCCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGT                                                                                                                               <  SRR19973066.2808304/150‑1 (MQ=60)
                     CAATCCGCCGAAGAGTATCGCCAGCTAATCATCGCCTACCAGAACGGCGCGCCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTT                                                                                                                              >  SRR19973066.3130183/1‑150 (MQ=60)
                     CAATCCGCCGAAGAGTATCGCCAGCTAATCATCGCCTACCAGAACGGCGCGCCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTT                                                                                                                              >  SRR19973066.2212193/1‑150 (MQ=60)
                     CAATCCGCCGAAGAGTATCGCCAGCTAATCATCGCCTACCAGAACGGCGCGCCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTT                                                                                                                              <  SRR19973066.1603112/150‑1 (MQ=60)
                     CAATCCGCCGAAGAGTATCGCCAGCTAATCATCGCCTACCAGAACGGCGCGCCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTT                                                                                                                              <  SRR19973066.1603509/150‑1 (MQ=60)
                       ATCCGCCGAAGAGTATCGCCAGCTAATCATCGCCTACCAGAACGGCGCGCCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCA                                                                                                                            >  SRR19973066.131222/1‑150 (MQ=60)
                       ATCCGCCGAAGAGTATCGCCAGCTAATCATCGCCTACCAGAACGGCGCGCCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCA                                                                                                                            <  SRR19973066.1210644/150‑1 (MQ=60)
                        TCCGCCGAAGAGTATCGCCAGCTAATCATCGCCTACCAGAACGGCGCGCCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAG                                                                                                                           <  SRR19973066.3496344/150‑1 (MQ=60)
                        TCCGCCGAAGAGTATCGCCAGCTAATCATCGCCTACCAGAACGGCGCGCCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAG                                                                                                                           <  SRR19973066.746511/150‑1 (MQ=60)
                          CGCCGAAGAGTATCGCCAGCTAATCATCGCCTACCAGAACGGCGCGCCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCG                                                                                                                         <  SRR19973066.2566745/150‑1 (MQ=60)
                             CGAAGAGTATCGCCAGCTAATCATCGCCTACCAGAACGGCGCGCCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCA                                                                                                                      >  SRR19973066.260943/1‑150 (MQ=60)
                              GAAGAGTATCGCCAGCTAATCATCGCCTACCAGAACGGCGCGCCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAG                                                                                                                     >  SRR19973066.2193385/1‑150 (MQ=60)
                                 GAGTATCGCCAGCTAATCATCGCCTACCAGAACGGCGCGCCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCC                                                                                                                  <  SRR19973066.937997/150‑1 (MQ=60)
                                 GAGTATCGCCAGCTAATCATCGCCTACCAGAACGGCGCGCCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCACGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCC                                                                                                                  <  SRR19973066.1524103/150‑1 (MQ=60)
                                 GAGTATCGCCAGCTAATCATCGCCTACCAGAACGGCGCGCCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCACGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCC                                                                                                                  <  SRR19973066.1524081/150‑1 (MQ=60)
                                  AGTATCGCCAGCTAATCATCGCCTACCAGAACGGCGCGCCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCG                                                                                                                 >  SRR19973066.808263/1‑150 (MQ=60)
                                  AGTATCGCCAGCTAATCATCGCCTACCAGAACGGCGCGCCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCG                                                                                                                 >  SRR19973066.808251/1‑150 (MQ=60)
                                       CGCCAGCTAATCATCGCCTACCAGAACGGCGCGCCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCT                                                                                                            <  SRR19973066.1681868/150‑1 (MQ=60)
                                       CGCCAGCTAATCATCGCCTACCAGAACGGCGCGCCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCT                                                                                                            <  SRR19973066.3458359/150‑1 (MQ=60)
                                        GCCAGCTAATCATCGCCTACCAGAACGGCGCGCCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTA                                                                                                           <  SRR19973066.3392764/150‑1 (MQ=60)
                                         CCAGCTAATCATCGCCTACCAGAACGGCGCGCCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAA                                                                                                          >  SRR19973066.3090532/1‑150 (MQ=60)
                                          CAGCTAATCATCGCCTACCAGAACGGCGCGCCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAAC                                                                                                         <  SRR19973066.357495/150‑1 (MQ=60)
                                          CAGCTAATCATCGCCGACCAGAACGGCGCGCCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAAC                                                                                                         <  SRR19973066.356672/150‑1 (MQ=60)
                                              TAATCATCGCCTACCAGAACGGCGCGCCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTA                                                                                                     >  SRR19973066.3111400/1‑150 (MQ=60)
                                                ATCATCGCCTACCAGAACGGCGCGCCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATC                                                                                                   >  SRR19973066.2206291/1‑150 (MQ=60)
                                                     CGCCTACCAGAACGGCGCGCCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCAC                                                                                              <  SRR19973066.2668110/150‑1 (MQ=60)
                                                      GCCTACCAGAACGGCGCGCCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGACAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACC                                                                                             <  SRR19973066.978029/150‑1 (MQ=60)
                                                      GCCTACCAGAACGGCGCGCCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACC                                                                                             <  SRR19973066.978064/150‑1 (MQ=60)
                                                      GCCTACCAGAACGGCGCGCCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACC                                                                                             <  SRR19973066.978805/150‑1 (MQ=60)
                                                         TACCAGAACGGCGCGCCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCC                                                                                          >  SRR19973066.752905/1‑150 (MQ=60)
                                                          ACCAGAACGGCGCGCCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCG                                                                                         >  SRR19973066.18570/1‑150 (MQ=60)
                                                            CAGAACGGCGCGCCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGAC                                                                                       >  SRR19973066.3427695/1‑150 (MQ=60)
                                                              GAACGGCGCGCCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCAGTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGACAG                                                                                     <  SRR19973066.349643/150‑1 (MQ=60)
                                                                     GCGCCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGACAGCATTCGG                                                                              <  SRR19973066.2448310/150‑1 (MQ=60)
                                                                       GCCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGACAGCATTCGGCA                                                                            >  SRR19973066.1475111/1‑150 (MQ=60)
                                                                        CCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGACAGCATTCGGCAG                                                                           <  SRR19973066.3424988/150‑1 (MQ=60)
                                                                        CCAAGTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGACAGCATTCGGCAG                                                                           <  SRR19973066.1646707/150‑1 (MQ=60)
                                                                         CAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGACAGCATTCGGCAGA                                                                          >  SRR19973066.1327166/1‑150 (MQ=60)
                                                                         CAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGACAGCATTCGGCAGA                                                                          >  SRR19973066.3217370/1‑150 (MQ=60)
                                                                           ATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGACAGCATTCGGCAGATG                                                                        <  SRR19973066.141606/150‑1 (MQ=60)
                                                                                 CTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGACAGCATTCGGCAGATGCTGCCA                                                                  <  SRR19973066.1653413/150‑1 (MQ=60)
                                                                                 CTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGACAGCATTCGGCAGATGCTGCCA                                                                  >  SRR19973066.1541428/1‑150 (MQ=60)
                                                                                 CTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGACAGCATTCGGCAGATGCTGCCA                                                                  >  SRR19973066.1541455/1‑150 (MQ=60)
                                                                                 CTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGACAGCATTCGGCAGATGCTGCCA                                                                  >  SRR19973066.1541480/1‑150 (MQ=60)
                                                                                 CTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGACAGCATTCGGCAGATGCTGCCA                                                                  <  SRR19973066.1652661/150‑1 (MQ=60)
                                                                                 CTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGACAGCATTCGGCAGATGCTGCCA                                                                  >  SRR19973066.2852868/1‑150 (MQ=60)
                                                                                 CTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGACAGCATTCGGCAGATGCTGCCA                                                                  >  SRR19973066.2852874/1‑150 (MQ=60)
                                                                                  TGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGACAGCATTCGGCAGATGCTGCCAC                                                                 >  SRR19973066.1627009/1‑150 (MQ=60)
                                                                                  TGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGACAGCATTCGGCAGATGCTGCCAC                                                                 >  SRR19973066.3188974/1‑150 (MQ=60)
                                                                                  TGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGACAGCATTCGGCAGATGCTGCCAC                                                                 >  SRR19973066.3189208/1‑150 (MQ=60)
                                                                                           TCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGACAGCATTCGGCAGATGCTGCCACAGCTCACTG                                                        <  SRR19973066.865486/150‑1 (MQ=60)
                                                                                           TCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGACAGCATTCGGCAGATGCTGCCACAGCTCACTG                                                        <  SRR19973066.3363434/150‑1 (MQ=60)
                                                                                               AACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGACAGCATTCGGCAGATGCTGCCACAGCTCACTGAGAG                                                    <  SRR19973066.3227801/150‑1 (MQ=60)
                                                                                                ACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGATTGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGACAGCATTCGGCAGATGCTGCCACAGCTCACTGAGAGT                                                   <  SRR19973066.1948523/150‑1 (MQ=60)
                                                                                                ACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGACAGCATTCGGCAGATGCTGCCACAGCTCACTGAGAGT                                                   <  SRR19973066.470801/150‑1 (MQ=60)
                                                                                                  TGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGACAGCATTCGGCAGATGCTGCCACAGCTCACTGAGAGTCT                                                 <  SRR19973066.1179981/150‑1 (MQ=60)
                                                                                                  TGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGACAGCATTCGGCAGATGCTGCCACAGCTCACTGAGAGTCT                                                 >  SRR19973066.895711/1‑150 (MQ=60)
                                                                                                    TAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGACAGCATTCGGCAGATGCTGCCACAGCTCACTGAGAGTCTGC                                               >  SRR19973066.3156898/1‑150 (MQ=60)
                                                                                                    TAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGACAGCATTCGGCAGATGCTGCCACAGCTCACTGAGAGTCTGC                                               >  SRR19973066.3156336/1‑150 (MQ=60)
                                                                                                     AGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGACAGCATTCGGCAGATGCTGCCACAGCTCACTGAGAGTCTGCC                                              <  SRR19973066.1544056/150‑1 (MQ=60)
                                                                                                          AAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGACAGCATTCGGCAGATGCTGCCACAGCTCACTGAGAGTCTGCCGAAAT                                         <  SRR19973066.1963351/150‑1 (MQ=60)
                                                                                                          AAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGACAGCATTCGGCAGATGCTGCCACAGCTCACTGAGAGTCTGCCGAAAT                                         <  SRR19973066.271657/150‑1 (MQ=60)
                                                                                                          AAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGACAGCATTCGGCAGATGCTGCCACAGCTCACTGAGAGTCTGCCGAAAT                                         <  SRR19973066.2108414/150‑1 (MQ=60)
                                                                                                          AAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGACAGCATTCGGCAGATGCTGCCACAGCTCACTGAGAGTCTGCCGAAAT                                         <  SRR19973066.1909723/150‑1 (MQ=60)
                                                                                                             GTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGACAGCATTCGGCAGATGCTGCCACAGCTCACTGAGAGTCTGCCGAAATCGG                                      <  SRR19973066.2624684/150‑1 (MQ=60)
                                                                                                               GCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGACAGCATTCGGCAGATGCTGCCACAGCTCACTGAGAGTCTGCCGAAATCGGTG                                    <  SRR19973066.919546/150‑1 (MQ=60)
                                                                                                                 AGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGACAGCATTCGGCAGATGCTGCCACAGCTCACTGAGAGTCTGCCGAAATCGGTGAA                                  >  SRR19973066.2634357/1‑150 (MQ=60)
                                                                                                                    AAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGACAGCATTCGGCAGATGCTGCCACAGCTCACTGAGAGTCTGCCGAAATCGGTGAAGGT                               >  SRR19973066.1404876/1‑150 (MQ=60)
                                                                                                                      ACAGCTGGCTCGGCGCGTGGGCGAACAAATTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGACAGCATTCGGCAGATGCTGCCACAGCTCACTGAGAGTCTGCCGAAATCGGTGAAGGTGA                             <  SRR19973066.3528688/150‑1 (MQ=60)
                                                                                                                      ACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGACAGCATTCGGCAGATGCTGCCACAGCTCACTGAGAGTCTGCCGAAATCGGTGAAGGTGA                             <  SRR19973066.2283299/150‑1 (MQ=60)
                                                                                                                           TGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGACAGCATTCGGCAGATGCTGCCACAGCTCACTGAGAGTCTGCCGAAATCGGTGAAGGTGACAGTG                        <  SRR19973066.1622780/150‑1 (MQ=60)
                                                                                                                                CGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGACAGCATTCGGCAGATGCTGCCACAGCTCACTGAGAGTCTGCCGAAATCGGTGAAGGTGACAGTGCTTTC                   >  SRR19973066.1958590/1‑150 (MQ=60)
                                                                                                                                 GGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGACAGCATTCGGCAGATGCTGCCACAGCTCACTGAGAGTCTGCCGAAATCGGTGAAGGTGACAGTGCTTTCC                  <  SRR19973066.1604532/150‑1 (MQ=60)
                                                                                                                                 GGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGACAGCATTCGGCAGATGCTGCCACAGCTCACTGAGAGTCTGCCGAAATCGGTGAAGGTGACAGTGCTTTCC                  <  SRR19973066.2869312/150‑1 (MQ=60)
                                                                                                                                  GCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGACAGCATTCGGCAGATGCTGCCACAGCTCACTGAGAGTCTGCCGAAATCGGTGAAGGTGACAGTGCTTTCCG                 <  SRR19973066.2638196/150‑1 (MQ=60)
                                                                                                                                     CGTGTGCGAACATAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGACAGAATTCGGCAGATGCTGCCACAGCTCACTGAGAGTCTGCCGAAATCTGTGAAGGTGACAGTGCTTTCCGATC              <  SRR19973066.325940/150‑1 (MQ=60)
                                                                                                                                     CGTGGGCGAACAAAGTACATGCGATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTATCATTATCTCCACCGCCGACAGCATTCGGCAGATGCTGCCACAGCTCACTGAGAGTCTGCCGATATCGGTGAAGGTGACAGTGCTTTCCGATC              <  SRR19973066.2132306/150‑1 (MQ=60)
                                                                                                                                      GTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGACAGCATTCGGCAGATGCTGCCACAGCTCACTGAGAGTCTGCCGAAATCGGTGAAGGTGACAGTGCTTTCCGATCG             <  SRR19973066.158128/150‑1 (MQ=60)
                                                                                                                                      GTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGACAGCATTCGGCAGATGCTGCCACAGCTCACTGAGAGTCTGCCGAAATCGGTGAAGGTGACAGTGCTTTCCGATCG             <  SRR19973066.1916924/150‑1 (MQ=60)
                                                                                                                                      GTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGACAGCATTCGGCAGATGCTGCCACAGCTCACTGAGAGTCTGCCGAAATCGGTGAAGGTGACAGTGCTTTCCGATCG             <  SRR19973066.3447261/150‑1 (MQ=60)
                                                                                                                                        GGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGACAGCATTCGGCAGATGCTGCCACAGCTCACTGAGAGTCTGCCGAAATCGGTGAAGGTGACAGTGCTTTCCGATCGCA           >  SRR19973066.1056167/1‑150 (MQ=60)
                                                                                                                                            GAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGACAGCATTCGGCAGATGCTGCCACAGCTCACTGAGAGTCTGCCGAAATCGGTGAAGGTGACAGTGCTTTCCGATCGCACCAC       >  SRR19973066.583708/1‑150 (MQ=60)
                                                                                                                                            GAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGACAGCATTCGGCAGATGCTGCCACAGCTCACTGAGAGTCTGCCGAAATCGGTGAAGGTGACAGTGCTTTCCGATCGCACCAC       >  SRR19973066.2910944/1‑150 (MQ=60)
                                                                                                                                            GAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGACAGCATTCGGCAGATGCTGCCACAGCTCACTGAGAGTCTGCCGAAATCGGTGAAGGTGACAGTGCTTTCCGATCGCACCAC       >  SRR19973066.2910976/1‑150 (MQ=60)
                                                                                                                                              ACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGACAGCATTCGCCACATGCTGCCACAGCTCACTGAGAGTCTGCCGAAATCGGTGAAGGTGACAGTGCTTTCCGATCGCACCACCA     >  SRR19973066.2123637/1‑150 (MQ=60)
                                                                                                                                                AAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGACAGCATTCGGCAGATGCTGCCACAGCTCACTGAGAGTCTGCCGAAATCGGTGAAGGTGACAGTGCTTTCCGATCGCACCACCAAT   <  SRR19973066.72460/150‑1 (MQ=60)
                                                                                                                                                AAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGACAGCATTCGGCAGATGCTGCCACAGCTCACTGAGAGTCTGCCGAAATCGGTGAAGGTGACAGTGCTTTCCGATCGCACCACCAAT   >  SRR19973066.3459262/1‑150 (MQ=60)
                                                                                                                                                 AAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGACAGCATTCGGCAGATGCTGCCACAGCTCACTGAGAGTCTGCCGAAATCGGTGAAGGTGACAGTGCTTTCCGATCGCACCACCAATA  <  SRR19973066.2313460/150‑1 (MQ=60)
                                                                                                                                                    |                                                                                                                                                  
CTTTCCGCGAACGACCAGATGCAATCCGCCGAAGAGTATCGCCAGCTAATCATCGCCTACCAGAACGGCGCGCCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGAACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGACAGCATTCGGCAGATGCTGCCACAGCTCACTGAGAGTCTGCCGAAATCGGTGAAGGTGACAGTGCTTTCCGATCGCACCACCAATA  >  CP009273/2149455‑2149749

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑