Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F394 I0 R1 1342 215.0 7134094 96.2% 6862998 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,370,987 C→A 100% E29* (GAA→TAA)  menH ← 2‑succinyl‑6‑hydroxy‑2, 4‑cyclohexadiene‑1‑carboxylate synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,370,9870CA100.0% 770.0 / NA 236E29* (GAA→TAA) menH2‑succinyl‑6‑hydroxy‑2, 4‑cyclohexadiene‑1‑carboxylate synthase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (127/109);  total (127/109)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 236 total aligned reads displayed.

TGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTCGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGCTTACCTGCGCCAGAAGTTGCTGGAGCGTTTGCGCACCATCGGTGTCGTTAACC  >  CP009273/2370843‑2371131
                                                                                                                                                |                                                                                                                                                
tgttgtAACTAACCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTg                                                                                                                                               <  1:356731/148‑1 (MQ=255)
 gttgtAACTAACCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTgg                                                                                                                                              >  2:3247461/1‑148 (MQ=255)
      aactaacCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATc                                                                                                                                         >  1:2606067/1‑148 (MQ=255)
      aactaacCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATc                                                                                                                                         >  1:60228/1‑148 (MQ=255)
      aactaacCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATc                                                                                                                                         >  1:59409/1‑148 (MQ=255)
       actaacCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCg                                                                                                                                        >  1:2666835/1‑148 (MQ=255)
        ctaacCAATGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGc                                                                                                                                       <  1:440451/148‑1 (MQ=255)
        ctaacCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGc                                                                                                                                       >  1:1437808/1‑148 (MQ=255)
         taacCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGcc                                                                                                                                      >  2:1714300/1‑148 (MQ=255)
          aacCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCg                                                                                                                                     >  2:437512/1‑148 (MQ=255)
          aacCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCg                                                                                                                                     >  1:1865328/1‑148 (MQ=255)
              aaGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGaaa                                                                                                                                 >  1:3375306/1‑148 (MQ=255)
               agtttttACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGaaaa                                                                                                                                <  1:390717/145‑1 (MQ=255)
               aGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGaaaa                                                                                                                                <  1:2387171/148‑1 (MQ=255)
                 gTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAAc                                                                                                                              >  1:2493551/1‑148 (MQ=255)
                 gTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAAc                                                                                                                              >  1:2186083/1‑148 (MQ=255)
                 gTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAAc                                                                                                                              >  1:849239/1‑148 (MQ=255)
                  ttttACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAAcc                                                                                                                             >  1:3229062/1‑148 (MQ=255)
                   tttACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCg                                                                                                                            >  1:1885983/1‑148 (MQ=255)
                           gTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCaaaaa                                                                                                                    >  1:1519797/1‑148 (MQ=255)
                            tAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAac                                                                                                                   >  1:544438/1‑148 (MQ=255)
                            tAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAac                                                                                                                   >  1:436684/1‑148 (MQ=255)
                               gTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACAcc                                                                                                                >  1:1432410/1‑148 (MQ=255)
                               gTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACAcc                                                                                                                <  2:711338/148‑1 (MQ=255)
                                  ggTTACATCATAAAATCCATCGACGATAATCGCCGCCGAACCACCGTGACGTGGGAGATAAACATACAACCTTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACAccagc                                                                                                             <  1:978303/148‑1 (MQ=255)
                                  ggTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACAccagc                                                                                                             >  2:651078/1‑148 (MQ=255)
                                   gTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACAccagcc                                                                                                            <  2:872149/148‑1 (MQ=255)
                                    tGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACAccagcca                                                                                                           >  1:404875/1‑148 (MQ=255)
                                      aCATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAgg                                                                                                         >  1:610793/1‑148 (MQ=255)
                                      aCATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAgg                                                                                                         >  2:1045873/1‑148 (MQ=255)
                                      aCATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAgg                                                                                                         >  1:3033592/1‑148 (MQ=255)
                                         tcatcaAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTa                                                                                                      >  2:2391348/1‑148 (MQ=255)
                                          catcaAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTaa                                                                                                     <  2:1145242/148‑1 (MQ=255)
                                            tcaAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAAc                                                                                                   <  2:1473297/148‑1 (MQ=255)
                                            taaaaTCCAGCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAAc                                                                                                   <  1:906568/146‑1 (MQ=255)
                                             caAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAAcc                                                                                                  <  2:1670271/148‑1 (MQ=255)
                                               aaTCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTg                                                                                                <  1:934022/148‑1 (MQ=255)
                                                aTCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTgg                                                                                               >  1:2844731/1‑148 (MQ=255)
                                                aTCCAGCGACGCGACTCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTgg                                                                                               <  2:2350800/148‑1 (MQ=255)
                                                 tCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGt                                                                                              <  2:1940213/148‑1 (MQ=255)
                                                   cATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGttt                                                                                            <  2:1672995/148‑1 (MQ=255)
                                                   cATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGttt                                                                                            <  2:3414019/148‑1 (MQ=255)
                                                   cATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGttt                                                                                            <  2:534371/148‑1 (MQ=255)
                                                    aTCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGtttt                                                                                           <  1:2524929/148‑1 (MQ=255)
                                                     tCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTAGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTc                                                                                          <  1:100466/148‑1 (MQ=255)
                                                      cGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTcc                                                                                         >  1:3448320/1‑148 (MQ=255)
                                                      cGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTcc                                                                                         >  1:1481045/1‑148 (MQ=255)
                                                       gACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCg                                                                                        <  1:2455378/148‑1 (MQ=255)
                                                         cGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCgtg                                                                                      >  1:1197109/1‑148 (MQ=255)
                                                         cGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCgtg                                                                                      <  1:30365/148‑1 (MQ=255)
                                                          gCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCgtgt                                                                                     <  1:2535386/148‑1 (MQ=255)
                                                          gCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCgtgt                                                                                     <  1:1295789/148‑1 (MQ=255)
                                                           cTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGtt                                                                                    <  2:1298037/148‑1 (MQ=255)
                                                            tAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGttt                                                                                   <  2:1982297/148‑1 (MQ=255)
                                                            tAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGttt                                                                                   <  2:2919204/148‑1 (MQ=255)
                                                            tAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGttt                                                                                   <  2:1140638/148‑1 (MQ=255)
                                                                cgccgcCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGcc                                                                               <  1:3337512/148‑1 (MQ=255)
                                                                      cgAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGcgcg                                                                         <  2:1117202/148‑1 (MQ=255)
                                                                      cgAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGcgcg                                                                         <  2:1781783/148‑1 (MQ=255)
                                                                        aaCCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTg                                                                       <  2:2333009/148‑1 (MQ=255)
                                                                         accaccGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGc                                                                      >  2:3103945/1‑148 (MQ=255)
                                                                            accGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAgg                                                                   <  1:1661248/148‑1 (MQ=255)
                                                                                tGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCa                                                               >  2:3299148/1‑148 (MQ=255)
                                                                                  aCCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATa                                                             >  2:1862680/1‑148 (MQ=255)
                                                                                   ccTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATaa                                                            <  2:3367618/148‑1 (MQ=255)
                                                                                       ggAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATgg                                                        >  2:207536/1‑148 (MQ=255)
                                                                                        gagaTCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGc                                                       <  2:1437660/148‑1 (MQ=255)
                                                                                        gagaTCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGc                                                       <  2:3229062/148‑1 (MQ=255)
                                                                                           aTCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGCTTa                                                    >  1:1307782/1‑148 (MQ=255)
                                                                                           aTCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGCTTa                                                    >  1:735766/1‑148 (MQ=255)
                                                                                           aTCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGCTTa                                                    >  2:1154484/1‑148 (MQ=255)
                                                                                           aTCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGCTTa                                                    >  2:1084766/1‑148 (MQ=255)
                                                                                            tCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGCTTAc                                                   <  1:1056700/148‑1 (MQ=255)
                                                                                             cAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGCTTAcc                                                  >  1:918308/1‑148 (MQ=255)
                                                                                               aCATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGCTTACCTg                                                >  2:838496/1‑148 (MQ=255)
                                                                                               aCATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGCTTACCTg                                                >  2:2625267/1‑148 (MQ=255)
                                                                                                  tACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGCTTACCTgcgc                                             >  1:1898817/1‑148 (MQ=255)
                                                                                                       ccGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGCTTACCTGCGCCAGaa                                        <  1:1200548/148‑1 (MQ=255)
                                                                                                       ccGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGCTTACCTGCGCCAGaa                                        >  2:1582528/1‑148 (MQ=255)
                                                                                                        cGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGCTTACCTGCGCCAGAAg                                       <  2:58040/148‑1 (MQ=255)
                                                                                                          tGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGCTTACCTGCGCCAGAAGtt                                     >  1:141065/1‑148 (MQ=255)
                                                                                                              tagtCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCACAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGCTTACCTGCGCCAGAAGTTGCTg                                 <  2:3561629/148‑1 (MQ=255)
                                                                                                              tagtCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGCTTACCTGCGCCAGAAGTTGCTg                                 >  2:1743739/1‑148 (MQ=255)
                                                                                                                 tCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGCTTACCTGCGCCAGAAGTTGCTGGAg                              >  2:3524788/1‑148 (MQ=255)
                                                                                                                 tCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGCTTACCTGCGCCAGAAGTTGCTGGAg                              <  1:1931839/148‑1 (MQ=255)
                                                                                                                        aCGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGCTTACCTGCGCCAGAAGTTGCTGGAGCGTTTgc                       >  1:3181747/1‑148 (MQ=255)
                                                                                                                         cGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGCTTACCTGCGCCAGAAGTTGCTGGAGCGTTTgcg                      >  1:2444948/1‑148 (MQ=255)
                                                                                                                         cGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGCTTACCTGCGCCAGAAGTTGCTGGAGCGTTTgcg                      >  2:1925056/1‑148 (MQ=255)
                                                                                                                           ccTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGCTTACCTGCGCCAGAAGTTGCTGGAGCGTTTGCGCa                    >  1:1337831/1‑148 (MQ=255)
                                                                                                                              cGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGCTTACCTGCGCCAGAAGTTGCTGGAGCGTTTGCGCACCa                 >  2:3271744/1‑148 (MQ=255)
                                                                                                                              cGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGCTTACCTGCGCCAGAAGTTGCTGGAGCGTTTGCGCACCa                 >  2:3245071/1‑148 (MQ=255)
                                                                                                                                cccACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGCTTACCTGCGCCAGAAGTTGCTGGAGCGTTTGCGCACCATc               >  1:1679735/1‑148 (MQ=255)
                                                                                                                                 ccACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGCTTACCTGCGCCAGAAGTTGCTGGAGCGTTTGCGCACCATCg              <  1:1154484/148‑1 (MQ=255)
                                                                                                                                 ccACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGCTTACCTGCGCCAGAAGTTGCTGGAGCGTTTGCGCACCATCg              <  1:1084766/148‑1 (MQ=255)
                                                                                                                                   aCTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGCTTACCTGCGCCAGAAGTTGCTGGAGCGTTTGCGCACCATCGgt            <  1:2174413/148‑1 (MQ=255)
                                                                                                                                   aCTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGCTTACCTGCGCCAGAAGTTGCTGGAGCGTTTGCGCACCATCGgt            >  2:2595339/1‑148 (MQ=255)
                                                                                                                                      tcttGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGCTTACCTGCGCCAGAAGTTGCTGGAGCGTTTGCGCACCATCGGTGTc         >  1:2714219/1‑148 (MQ=255)
                                                                                                                                      tcttGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGCTTACCTGCGCCAGAAGTTGCTGGAGCGTTTGCGCACCATCGGTGTc         >  2:555713/1‑148 (MQ=255)
                                                                                                                                        ttGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGCTTACCTGCGCCAGAAGTTGCTGGAGCGTTTGCGCACCATCGGTGTCGt       <  2:141065/148‑1 (MQ=255)
                                                                                                                                             aTTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGCTTACCTGCGCCAGAAGTTGCTGGAGCGTTTGCGCACCATCGGTGTCGTTAacc  >  2:847621/1‑148 (MQ=255)
                                                                                                                                                |                                                                                                                                                
TGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTCGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGCTTACCTGCGCCAGAAGTTGCTGGAGCGTTTGCGCACCATCGGTGTCGTTAACC  >  CP009273/2370843‑2371131

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
AAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTCGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGCTTACCTGCGCCAGAAGTTGCTGGAGCGTTTGCGCACCATCGGTGTCGTTAACCACC  >  CP009273/2370838‑2371134
                                                                                                                                                     |                                                                                                                                                   
AAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACTCCTCGCCCACTTCTTGCCATTA                                                                                                                                                     >  SRR19973066.153327/1‑150 (MQ=60)
 AGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAG                                                                                                                                                    <  SRR19973066.1903893/150‑1 (MQ=60)
    ATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGG                                                                                                                                                 >  SRR19973066.414509/1‑150 (MQ=60)
     TGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGC                                                                                                                                                <  SRR19973066.359512/150‑1 (MQ=60)
      GTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCA                                                                                                                                               <  SRR19973066.415993/150‑1 (MQ=60)
         GTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATC                                                                                                                                            >  SRR19973066.2623691/1‑150 (MQ=60)
         GTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATC                                                                                                                                            >  SRR19973066.60973/1‑150 (MQ=60)
         GTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATC                                                                                                                                            >  SRR19973066.60786/1‑150 (MQ=60)
         GTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATC                                                                                                                                            >  SRR19973066.60145/1‑150 (MQ=60)
         GTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATC                                                                                                                                            >  SRR19973066.2763153/1‑150 (MQ=60)
          TAACTAACCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCG                                                                                                                                           >  SRR19973066.2684679/1‑150 (MQ=60)
           AACTAACCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGC                                                                                                                                          >  SRR19973066.1448051/1‑150 (MQ=60)
           AACTAACCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGC                                                                                                                                          >  SRR19973066.1447902/1‑150 (MQ=60)
             CTAACCACGGTTTTACGCAGTAAGTCGGTGACATCATAAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCG                                                                                                                                        <  SRR19973066.3433545/150‑1 (MQ=60)
             CTAACCAATGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCG                                                                                                                                        <  SRR19973066.443854/150‑1 (MQ=60)
             CTAACCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCG                                                                                                                                        >  SRR19973066.1878654/1‑150 (MQ=60)
             CTAACCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCG                                                                                                                                        <  SRR19973066.443897/150‑1 (MQ=60)
                ACCACGGTTTTACGCAGTATGTCGGTGACATCATCAAACCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAA                                                                                                                                     <  SRR19973066.1033486/150‑1 (MQ=60)
                 CCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAA                                                                                                                                    >  SRR19973066.3397813/1‑150 (MQ=60)
                   AAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAA                                                                                                                                  >  SRR19973066.2515958/1‑150 (MQ=60)
                    AGTTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAAC                                                                                                                                 <  SRR19973066.393798/150‑1 (MQ=60)
                    AGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAAC                                                                                                                                 >  SRR19973066.2201253/1‑150 (MQ=60)
                    AGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAAC                                                                                                                                 >  SRR19973066.394736/1‑150 (MQ=60)
                    AGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAAC                                                                                                                                 <  SRR19973066.2403252/150‑1 (MQ=60)
                    AGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAAC                                                                                                                                 >  SRR19973066.2436448/1‑150 (MQ=60)
                    AGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAAC                                                                                                                                 >  SRR19973066.2510552/1‑150 (MQ=60)
                    AGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAAC                                                                                                                                 >  SRR19973066.855388/1‑150 (MQ=60)
                     GGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACC                                                                                                                                >  SRR19973066.3250398/1‑150 (MQ=60)
                     GGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACC                                                                                                                                <  SRR19973066.3116522/150‑1 (MQ=60)
                      GTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCG                                                                                                                               >  SRR19973066.649241/1‑150 (MQ=60)
                      GTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCG                                                                                                                               >  SRR19973066.1900167/1‑150 (MQ=60)
                      GTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCG                                                                                                                               >  SRR19973066.1899429/1‑150 (MQ=60)
                      GTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCG                                                                                                                               >  SRR19973066.1898773/1‑150 (MQ=60)
                      GTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCG                                                                                                                               >  SRR19973066.267509/1‑150 (MQ=60)
                          TACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCA                                                                                                                           >  SRR19973066.549892/1‑150 (MQ=60)
                          TACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCA                                                                                                                           >  SRR19973066.2354193/1‑150 (MQ=60)
                              CAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAA                                                                                                                       >  SRR19973066.1530513/1‑150 (MQ=60)
                              CAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAA                                                                                                                       >  SRR19973066.1307440/1‑150 (MQ=60)
                               AGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAAC                                                                                                                      >  SRR19973066.548370/1‑150 (MQ=60)
                               AGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAAC                                                                                                                      >  SRR19973066.440067/1‑150 (MQ=60)
                               AGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAAC                                                                                                                      >  SRR19973066.440065/1‑150 (MQ=60)
                                 TAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACAC                                                                                                                    <  SRR19973066.3381964/150‑1 (MQ=60)
                                  AAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACC                                                                                                                   >  SRR19973066.1442630/1‑150 (MQ=60)
                                  AAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACC                                                                                                                   >  SRR19973066.1442619/1‑150 (MQ=60)
                                  AAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACC                                                                                                                   <  SRR19973066.1372164/150‑1 (MQ=60)
                                    GTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAG                                                                                                                 <  SRR19973066.2588704/150‑1 (MQ=60)
                                     TCGGTGACATAATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGC                                                                                                                <  SRR19973066.970777/150‑1 (MQ=60)
                                       GGTTACATCATAAAATCCATCGACGATAATCGCCGCCGAACCACCGTGACGTGGGAGATAAACATACAACCTTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCA                                                                                                              <  SRR19973066.985515/150‑1 (MQ=60)
                                       GGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCA                                                                                                              >  SRR19973066.408060/1‑150 (MQ=60)
                                         TGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGG                                                                                                            >  SRR19973066.3053864/1‑150 (MQ=60)
                                         TGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGG                                                                                                            >  SRR19973066.615052/1‑150 (MQ=60)
                                            CATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTA                                                                                                         >  SRR19973066.3093509/1‑150 (MQ=60)
                                             ATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAA                                                                                                        <  SRR19973066.1202489/150‑1 (MQ=60)
                                                ATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACC                                                                                                     >  SRR19973066.2499282/1‑150 (MQ=60)
                                                ATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACC                                                                                                     >  SRR19973066.2499328/1‑150 (MQ=60)
                                                 TAAAATCCAGCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCT                                                                                                    <  SRR19973066.912970/150‑1 (MQ=60)
                                                   AAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGG                                                                                                  >  SRR19973066.1376666/1‑150 (MQ=60)
                                                   AAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGG                                                                                                  >  SRR19973066.2863848/1‑150 (MQ=60)
                                                    AATCCATCGACGCTACTCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGT                                                                                                 <  SRR19973066.940855/150‑1 (MQ=60)
                                                    AATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGT                                                                                                 <  SRR19973066.940805/150‑1 (MQ=60)
                                                     ATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTT                                                                                                <  SRR19973066.1086737/150‑1 (MQ=60)
                                                     ATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTT                                                                                                <  SRR19973066.905087/150‑1 (MQ=60)
                                                     ATCCATCGACGATAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTT                                                                                                <  SRR19973066.614703/150‑1 (MQ=60)
                                                         ATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCC                                                                                            >  SRR19973066.2705809/1‑150 (MQ=60)
                                                         ATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCC                                                                                            <  SRR19973066.2542167/150‑1 (MQ=60)
                                                         ATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCC                                                                                            <  SRR19973066.2542099/150‑1 (MQ=60)
                                                         ATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCC                                                                                            >  SRR19973066.1491452/1‑150 (MQ=60)
                                                         ATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCC                                                                                            >  SRR19973066.3471379/1‑150 (MQ=60)
                                                         ATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCC                                                                                            >  SRR19973066.3471376/1‑150 (MQ=60)
                                                          TCGACGCTAAGCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACGGTGAGTAGTAGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCG                                                                                           <  SRR19973066.101535/150‑1 (MQ=60)
                                                            GACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTG                                                                                         >  SRR19973066.1205468/1‑150 (MQ=60)
                                                            GACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTG                                                                                         >  SRR19973066.1206429/1‑150 (MQ=60)
                                                            GACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTG                                                                                         <  SRR19973066.2472109/150‑1 (MQ=60)
                                                              CGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTT                                                                                       <  SRR19973066.30772/150‑1 (MQ=60)
                                                              CGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTT                                                                                       <  SRR19973066.30784/150‑1 (MQ=60)
                                                               GCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTT                                                                                      <  SRR19973066.2552772/150‑1 (MQ=60)
                                                               GCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTT                                                                                      <  SRR19973066.2552620/150‑1 (MQ=60)
                                                               GCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTT                                                                                      <  SRR19973066.1305177/150‑1 (MQ=60)
                                                               GCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTT                                                                                      <  SRR19973066.1852507/150‑1 (MQ=60)
                                                                CTAATCGCCGCCGACCCACCGTGACCTGGGAGATCAACATACACCCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTT                                                                                     <  SRR19973066.694067/150‑1 (MQ=60)
                                                                CTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACACCCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTT                                                                                     <  SRR19973066.168816/150‑1 (MQ=60)
                                                                CTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTT                                                                                     <  SRR19973066.1616284/150‑1 (MQ=60)
                                                                CTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTT                                                                                     <  SRR19973066.2407439/150‑1 (MQ=60)
                                                                CTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTT                                                                                     <  SRR19973066.1500188/150‑1 (MQ=60)
                                                                     CGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTG                                                                                <  SRR19973066.3359725/150‑1 (MQ=60)
                                                                             AACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCA                                                                        >  SRR19973066.3327905/1‑150 (MQ=60)
                                                                             AACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCA                                                                        >  SRR19973066.58761/1‑150 (MQ=60)
                                                                                 ACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGAT                                                                    >  SRR19973066.2892457/1‑150 (MQ=60)
                                                                                 ACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGAT                                                                    <  SRR19973066.1673352/150‑1 (MQ=60)
                                                                                  CCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATC                                                                   <  SRR19973066.934850/150‑1 (MQ=60)
                                                                                              AGATCAACATTCAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGCTTA                                                       <  SRR19973066.895461/150‑1 (MQ=60)
                                                                                              AGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGCTTA                                                       >  SRR19973066.1318105/1‑150 (MQ=60)
                                                                                              AGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGCTTA                                                       >  SRR19973066.1317268/1‑150 (MQ=60)
                                                                                              AGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGCTTA                                                       <  SRR19973066.14911/150‑1 (MQ=60)
                                                                                              AGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGCTTA                                                       >  SRR19973066.741227/1‑150 (MQ=60)
                                                                                                ATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGCTTACC                                                     >  SRR19973066.1893648/1‑150 (MQ=60)
                                                                                                ATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGCTTACC                                                     >  SRR19973066.924893/1‑150 (MQ=60)
                                                                                                ATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGCTTACC                                                     >  SRR19973066.924902/1‑150 (MQ=60)
                                                                                                 TCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGCTTACCT                                                    <  SRR19973066.1064466/150‑1 (MQ=60)
                                                                                                  CAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGCTTACCTG                                                   >  SRR19973066.3531305/1‑150 (MQ=60)
                                                                                                     CATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGCTTACCTGCGC                                                >  SRR19973066.3368534/1‑150 (MQ=60)
                                                                                                     CATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGCTTACCTGCGC                                                >  SRR19973066.1912427/1‑150 (MQ=60)
                                                                                                     CATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGCTTACCTGCGC                                                >  SRR19973066.1912365/1‑150 (MQ=60)
                                                                                                     CATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGCTTACCTGCGC                                                >  SRR19973066.1586877/1‑150 (MQ=60)
                                                                                                            CCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGCTTACCTGCGCCAGAAGT                                         <  SRR19973066.1208913/150‑1 (MQ=60)
                                                                                                             CGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGCTTACCTGCGCCAGAAGTT                                        >  SRR19973066.142424/1‑150 (MQ=60)
                                                                                                             CGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGCTTACCTGCGCCAGAAGTT                                        >  SRR19973066.2068170/1‑150 (MQ=60)
                                                                                                                    AGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGCTTACCTGCGCCAGAAGTTGCTGGAG                                 >  SRR19973066.1485590/1‑150 (MQ=60)
                                                                                                                      TCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGCTTACCTGCGCCAGAAGTTGCTGGAGCG                               <  SRR19973066.1945553/150‑1 (MQ=60)
                                                                                                                       CGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGCTTACCTGCGCCAGAAGTTGCTGGAGCGT                              <  SRR19973066.2466251/150‑1 (MQ=60)
                                                                                                                           AAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGCTTACCTGCGCCAGAAGTTGCTGGAGCGTTTGC                          >  SRR19973066.3202916/1‑150 (MQ=60)
                                                                                                                           AAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGCTTACCTGCGCCAGAAGTTGCTGGAGCGTTTGC                          >  SRR19973066.3202911/1‑150 (MQ=60)
                                                                                                                            AACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGCTTACCTGCGCCAGAAGTTGCTGGAGCGTTTGCG                         >  SRR19973066.2461599/1‑150 (MQ=60)
                                                                                                                            AACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGCTTACCTGCGCCAGAAGTTGCTGGAGCGTTTGCG                         >  SRR19973066.2822276/1‑150 (MQ=60)
                                                                                                                              CGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGCTTACCTGCGCCAGAAGTTGCTGGAGCGTTTGCGCA                       >  SRR19973066.1347539/1‑150 (MQ=60)
                                                                                                                                   CGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGCTTACCTGCGCCAGAAGTTGCTGGAGCGTTTGCGCACCATC                  >  SRR19973066.1691959/1‑150 (MQ=60)
                                                                                                                                   CGCCCACTTCGTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGCTTACCTGCGCCAGAAGTTGCTGGAGCGTTTGCGCACCAAC                  <  SRR19973066.1284260/150‑1 (MQ=60)
                                                                                                                                    GCCCACTTCTTGCCATTAGTGTCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGCTTACCTGCGCCAGAAGTTGCTGGAGCGTTTGCGCACCATCG                 <  SRR19973066.2056613/150‑1 (MQ=60)
                                                                                                                                     CCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGCTTACCTGCGCCAGAAGTTGCTGGAGCGTTTGCGCACCATCGG                >  SRR19973066.1145971/1‑150 (MQ=60)
                                                                                                                                      CCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGCTTACCTGCGCCAGAAGTTGCTGGAGCGTTTGCGCACCATCGGT               <  SRR19973066.1092687/150‑1 (MQ=60)
                                                                                                                                      CCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGCTTACCTGCGCCAGAAGTTGCTGGAGCGTTTGCGCACCATCGGT               <  SRR19973066.1162698/150‑1 (MQ=60)
                                                                                                                                      CCACGTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGCTTACCTGCGCCAGAAGTTGCTGGAGCGTTTGCGCACCATCGGT               <  SRR19973066.1162689/150‑1 (MQ=60)
                                                                                                                                        ACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGCTTACCTGCGCCAGAAGTTGCTGGAGCGTTTGCGCACCATCGGTGT             <  SRR19973066.381998/150‑1 (MQ=60)
                                                                                                                                        ACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGCTTACCTGCGCCAGAAGTTGCTGGAGCGTTTGCGCACCATCGGTGT             <  SRR19973066.2189535/150‑1 (MQ=60)
                                                                                                                                        ACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGCTTACCTGCGCCAGAAGTTGCTGGAGCGTTTGCGCACCATCGGTGT             <  SRR19973066.2189516/150‑1 (MQ=60)
                                                                                                                                         CTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGCTTACCTGCGCCAGAAGTTGCTGGAGCGTTTGCGCACCATCGGTGTC            >  SRR19973066.2732453/1‑150 (MQ=60)
                                                                                                                                         CTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGCTTACCTGCGCCAGAAGTTGCTGGAGCGTTTGCGCACCATCGGTGTC            >  SRR19973066.1251090/1‑150 (MQ=60)
                                                                                                                                               GCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGCTTACCTGCGCCAGAAGTTGCTGGAGCGTTTGCGCACCATCGGTGTCGTTAAC      >  SRR19973066.819510/1‑150 (MQ=60)
                                                                                                                                                   TTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGCTTACCTGCGCCAGAAGTTGCTGGAGCGTTTGCGCACCATCGGTGTCGTTAACCACC  <  SRR19973066.2242282/150‑1 (MQ=60)
                                                                                                                                                     |                                                                                                                                                   
AAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTCGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGCTTACCTGCGCCAGAAGTTGCTGGAGCGTTTGCGCACCATCGGTGTCGTTAACCACC  >  CP009273/2370838‑2371134

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑