Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F142 I0 R1 859 274.6 8723680 96.4% 8409627 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,803,109 C→T 49.2% A128T (GCC→ACC)  yniB ← putative inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,803,1090CT49.2% 25.7 / inf 262A128T (GCC→ACC) yniBputative inner membrane protein
Reads supporting (aligned to +/- strand):  ref base C (82/51);  new base T (68/61);  total (150/112)
Fisher's exact test for biased strand distribution p-value = 1.70e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.88e-01

Only 100 of 267 total aligned reads displayed.

AAACCCAAGCAGAGAAAAGAACACATAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGCTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCAGTCCGACAAAAATCAGGATGAAAATCACCCAGAACATAACGTTCACGCCACCATTAAAATTGG  >  CP009273/1802960‑1803258
                                                                                                                                                     |                                                                                                                                                     
aaaCCCAAGCAGAGAAAAGAACACATAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGc                                                                                                                                                       <  1:91071/150‑1 (MQ=255)
 aaCCCAAGCAGAGAAAAGAACACATAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGCt                                                                                                                                                      <  1:347727/150‑1 (MQ=255)
    ccAAGCAGAGAAAAGAACACATAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGCtttt                                                                                                                                                   <  1:1987068/150‑1 (MQ=255)
    ccAAGCAGAGAAAAGAACACATAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGCtttt                                                                                                                                                   >  1:1212938/1‑150 (MQ=255)
      aaGCAGAGAAAAGAACACATAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTc                                                                                                                                                 <  1:3955399/150‑1 (MQ=255)
       aGCAGAGAAAAGAACACATAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCACTTTGGCTTTTTcc                                                                                                                                                >  1:3237806/1‑150 (MQ=255)
        gCAGAGAAAAGAACACATAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGCTTTTTCCa                                                                                                                                               <  1:1099243/150‑1 (MQ=255)
         cAGAGAAAAGAACACATAAACTGCAGCAATAAAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGCTTTTTCCAg                                                                                                                                              <  1:114527/150‑1 (MQ=255)
            agaAAAGAACACATAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAAt                                                                                                                                           <  2:2643559/150‑1 (MQ=255)
            agaAAAGAACACATAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGCTTTTTCCAGAAt                                                                                                                                           <  1:2882038/150‑1 (MQ=255)
            agaAAAGAACACATAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCCCTTCCCCTTTGGCTTTTTCCAGAAt                                                                                                                                           >  1:177124/1‑150 (MQ=255)
             gaAAAGAACACATAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATg                                                                                                                                          >  1:3913203/1‑150 (MQ=255)
             gaAAAGAACACATAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATg                                                                                                                                          >  1:1016801/1‑150 (MQ=255)
               aaaGAACACATAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGCTTTTTCCAGAATGAg                                                                                                                                        >  1:1374922/1‑150 (MQ=255)
               aaaGAACACATAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGCTTTTTCCAGAATGAg                                                                                                                                        >  1:1175702/1‑150 (MQ=255)
                aaGAACACATAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGt                                                                                                                                       >  1:853470/1‑150 (MQ=255)
                 aGAACACATAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACACTACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGtt                                                                                                                                      <  2:2769045/150‑1 (MQ=255)
                 aGAACACATAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGtt                                                                                                                                      >  1:471505/1‑150 (MQ=255)
                 aGAACACATAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGCTTTTTCCAGAATGAGtt                                                                                                                                      <  1:563296/150‑1 (MQ=255)
                    acacATAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGAt                                                                                                                                   <  2:2271455/150‑1 (MQ=255)
                    acacATAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGCTTTTTCCAGAATGAGTTGAt                                                                                                                                   <  1:1602772/150‑1 (MQ=255)
                     cacaTAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGCTTTTTCCAGAATGAGTTGATc                                                                                                                                  >  1:1859471/1‑150 (MQ=255)
                      acaTAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGCTTTTTCCAGAATGAGTTGATCt                                                                                                                                 <  2:2073408/150‑1 (MQ=255)
                       caTAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCtt                                                                                                                                <  1:1519956/150‑1 (MQ=255)
                       caTAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGCTTTTTCCAGAATGAGTTGATCtt                                                                                                                                <  2:3283998/150‑1 (MQ=255)
                        aTAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGCTTTTTCCAGAATGAGTTGATCTTc                                                                                                                               >  2:3658698/1‑150 (MQ=255)
                         tAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGCTTTTTCCAGAATGAGTTGATCTTCa                                                                                                                              <  2:2860263/150‑1 (MQ=255)
                             cTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAacac                                                                                                                          >  2:2947762/1‑150 (MQ=255)
                             cTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAacac                                                                                                                          >  1:333654/1‑150 (MQ=255)
                             cTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGCTTTTTCCAGAATGAGTTGATCTTCAacac                                                                                                                          >  2:379601/1‑150 (MQ=255)
                              tGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGCCTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGCTTTTTCCAGAATGAGTTGATCTTCAACAcc                                                                                                                         >  2:2046305/1‑150 (MQ=255)
                                 agcaATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGCTTTTTCCAGAATGAGTTGATCTTCAACACCTTc                                                                                                                      >  2:4114621/1‑150 (MQ=255)
                                  gcaATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTcc                                                                                                                     >  2:4355083/1‑150 (MQ=255)
                                     aTACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGCTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGc                                                                                                                  >  1:710780/1‑150 (MQ=255)
                                       aCAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCaa                                                                                                                >  1:1667341/1‑150 (MQ=255)
                                           aTAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCaagaag                                                                                                            >  2:4329425/1‑150 (MQ=255)
                                            tAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGCTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCACGAAgc                                                                                                           >  2:192980/1‑150 (MQ=255)
                                                 ggCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGCTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGcc                                                                                                      >  1:2970998/1‑150 (MQ=255)
                                                  gCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGCTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCt                                                                                                     <  1:779938/150‑1 (MQ=255)
                                                  gCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTGAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCt                                                                                                     <  2:3453332/150‑1 (MQ=255)
                                                   cAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTg                                                                                                    <  2:3615385/150‑1 (MQ=255)
                                                    aGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTgg                                                                                                   >  2:4298424/1‑150 (MQ=255)
                                                    aGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGCTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTgg                                                                                                   >  2:3533485/1‑150 (MQ=255)
                                                     ggATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGc                                                                                                  >  1:3102767/1‑150 (MQ=255)
                                                          taCAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGTACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCACCTTTGGCTTTTTCCAGAATGAGTTGATCTTCAAAACCTTCACGCAAGAAGCGCGCCTGGCGACTc                                                                                             >  1:805390/1‑150 (MQ=255)
                                                               tGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCg                                                                                        >  1:3421594/1‑150 (MQ=255)
                                                                   aaaaaCTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCa                                                                                    >  1:3866398/1‑150 (MQ=255)
                                                                   aaaaaCTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGCTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCa                                                                                    <  1:1164203/150‑1 (MQ=255)
                                                                   aaaaaCTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGCTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCa                                                                                    >  1:1427895/1‑150 (MQ=255)
                                                                       aCTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCgg                                                                                >  1:3918895/1‑150 (MQ=255)
                                                                            aGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATcc                                                                           >  1:1705238/1‑150 (MQ=255)
                                                                             ggAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGCTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCt                                                                          <  2:1220502/150‑1 (MQ=255)
                                                                               aaaaTAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGCTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGc                                                                        >  1:4343008/1‑150 (MQ=255)
                                                                                 aaTAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGCTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCaa                                                                      <  2:390981/150‑1 (MQ=255)
                                                                                  aTAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGCTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAAc                                                                     <  1:2537750/150‑1 (MQ=255)
                                                                                   tAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGCTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAACg                                                                    >  1:2261564/1‑150 (MQ=255)
                                                                                    aGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGCTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGc                                                                   >  1:3140271/1‑150 (MQ=255)
                                                                                    aGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGCTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGc                                                                   >  1:897901/1‑150 (MQ=255)
                                                                                     gTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGCTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGcc                                                                  <  2:2657800/150‑1 (MQ=255)
                                                                                       aTGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCAg                                                                >  2:831862/1‑150 (MQ=255)
                                                                                        tGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCAGt                                                               <  2:3181311/150‑1 (MQ=255)
                                                                                         ggTGCGTAACAACAATACGGGACTCAATTTGTACAATCGTTAGCCCCTCTTCCCCTTTGGCTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCTCGCCTGGCGACTCATCAGGGCACCGGAATCCTGCAACGCCAGTc                                                              >  2:3098943/1‑150 (MQ=255)
                                                                                            gCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCAGTCCGa                                                           <  2:3299675/150‑1 (MQ=255)
                                                                                             cGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGCTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCAGTCCGAc                                                          >  1:1020555/1‑150 (MQ=255)
                                                                                              ggAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCAGTCCGACa                                                         >  1:3995006/1‑150 (MQ=255)
                                                                                                   aacaaTACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCAGTCCGACAAAAAt                                                    >  1:1895577/1‑150 (MQ=255)
                                                                                                    acaaTACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCAGTCCGACAAAAATc                                                   <  2:3948267/150‑1 (MQ=255)
                                                                                                     caaTACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGCTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCAGTCCGACAAAAATCa                                                  >  2:1587219/1‑150 (MQ=255)
                                                                                                     caaTACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGCTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCAGTCCGACAAAAATCa                                                  >  2:1588053/1‑150 (MQ=255)
                                                                                                      aaTACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCAGTCCGACAAAAATCAg                                                 >  1:2404546/1‑150 (MQ=255)
                                                                                                         aCGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCAGTCCGACAAAAATCAGGAt                                              <  1:2470576/150‑1 (MQ=255)
                                                                                                          cGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGCTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCAGTCCGACAAAAATCAGGATg                                             <  1:897313/150‑1 (MQ=255)
                                                                                                          cGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGCTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCAGTCCGACAAAAATCAGGATg                                             >  1:4050526/1‑150 (MQ=255)
                                                                                                                tCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCAGTCCGACAAAAATCAGGATGAAAATc                                       <  1:993459/150‑1 (MQ=255)
                                                                                                                    tttGTTCACGCGTTAGCCCCTCTTCCCCTTTTGCTTTTTCCAGAATGAGTTGATCGTCAACACCTTCACGCAAGAAGCGCGCCTTGCGACTCATCCGGGCACCGGAATCCTGCAACGCCAGTCCGACAAAAATCAGGATGAAAATCAccc                                   <  2:2442701/150‑1 (MQ=255)
                                                                                                                    tttGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCAGTCCGACAAAAATCAGGATGAAAATCAccc                                   <  2:3920439/150‑1 (MQ=255)
                                                                                                                      tGTTCACGCGTTAGCCCCTCTTCCCCTTTGGCTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCAGTCCGACAAAAATCAGGATGAAAATCACCCAg                                 >  1:4274593/1‑150 (MQ=255)
                                                                                                                        ttCACGCGTTAGCCCCTCTTCCCCTTTGGCTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCAGTCCGACAAAAATCAGGATGAAAATCACCCAGaa                               <  2:2448339/150‑1 (MQ=255)
                                                                                                                         tCACGCGTTAGCCCCTCTTCCCCTTTGGCTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCAGTCCGACAAAAATCAGGATGAAAATCACCCAGAAc                              >  1:2282573/1‑150 (MQ=255)
                                                                                                                            cgcgTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCAGTCCGACAAAAATCAGGATGAAAATCACCCAGAACaaa                           >  1:3659660/1‑148 (MQ=255)
                                                                                                                            cgcgTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCAGTCCGACAAAAATCAGGATGAAAATCACCCAGAACATa                           <  1:3808107/150‑1 (MQ=255)
                                                                                                                            cgcgTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCAGTCCGACAAAAATCAGGATGAAAATCACCCAGAACATa                           <  1:1619642/150‑1 (MQ=255)
                                                                                                                            cgcgTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCAGTCCGACAAAAATCAGGATGAAAATCACCCAGAACATa                           <  1:2563298/150‑1 (MQ=255)
                                                                                                                             gcgTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCAGTCCGACAAAAATCAGGATGAAAATCACCCAGAACATaa                          <  2:2689190/150‑1 (MQ=255)
                                                                                                                              cgTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCAGTCCGACAAAAATCAGGATGAAAATCACCCAGAACATAAc                         <  2:118816/150‑1 (MQ=255)
                                                                                                                              cgTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCAGTCCGACAAAAATCAGGATGAAAATCACCCAGAACATAAc                         <  2:4311499/150‑1 (MQ=255)
                                                                                                                              cgTTAGCCCCTCTTCCCCTTTGGCTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCAGTCCGACAAAAATCAGGATGAAAATCACCCAGAACATAAc                         >  2:1564576/1‑150 (MQ=255)
                                                                                                                               gTTAGCCCCTCTTCCCCTTTGGCTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCAGTCCGACAAAAATCAGGATGAAAATCACCCAGAACATAACg                        >  1:2347330/1‑150 (MQ=255)
                                                                                                                                 tAGCCCCTCTTCCCCTTTGGCTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCAGTCCGACAAAAATCAGGATGAAAATCACCCAGAACATAACGtt                      >  2:1835581/1‑150 (MQ=255)
                                                                                                                                   gCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCAGTCCGACAAAAATCAGGATGAAAATCACCCAGAACATAACGTTCa                    <  2:1667263/150‑1 (MQ=255)
                                                                                                                                        tctTCCCCTTTGGCTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCAGTCCGACAAAAATCAGGATGAAAATCACCCAGAACATAACGTTCACGcca               >  1:1062816/1‑150 (MQ=255)
                                                                                                                                         ctTCCCCTTTGGCTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCAGTCCGACAAAAATCAGGATGAAAATCACCCAGAACATAACGTTCACGccac              >  1:2850174/1‑150 (MQ=255)
                                                                                                                                                tttGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCAGTCCGACAAAAATCAGGATGAAAATCACCCAGAACATAACGTTCACGCCACCATTaaa       >  2:2437638/1‑150 (MQ=255)
                                                                                                                                                tttGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCAGTCCGACAAAAATCAGGATGAAAATCACCCAGAACATAACGTTCACGCCACCATTaaa       >  1:2804608/1‑150 (MQ=255)
                                                                                                                                                tttGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCAGTCCGACAAAAATCAGGATGAAAATCACCCAGAACATAACGTTCACGCCACCATTaaa       >  2:2878449/1‑150 (MQ=255)
                                                                                                                                                tttGGCTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCAGTCCGACAAAAATCAGGATGAAAATCACCCAGAACATAACGTTCACGCCACCATTaaa       >  1:1338081/1‑150 (MQ=255)
                                                                                                                                                 ttGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCAGTCCGACAAAAATCAGGATGAAAATCACCCAGAACATAACGTTCACGCCACCATTaaaa      <  1:894964/150‑1 (MQ=255)
                                                                                                                                                   ggCTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCAGTCCGACAAAAATCAGGATGAAAATCACCCAGAACATAACGTTCACGCCACCATTAAAAtt    >  1:2115285/1‑150 (MQ=255)
                                                                                                                                                    gCTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCAGTCCGACAAAAATCAGGATGAAAATCACCCAGAACATAACGTTCACGCCACCATTAAAATTg   <  2:4030693/150‑1 (MQ=255)
                                                                                                                                                     cTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCAGTCCGACAAAAATCAGGATGAAAATCACCCAGAACATAACGTTCACGCCACCATTAAAATTgg  >  1:2152934/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
AAACCCAAGCAGAGAAAAGAACACATAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGCTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCAGTCCGACAAAAATCAGGATGAAAATCACCCAGAACATAACGTTCACGCCACCATTAAAATTGG  >  CP009273/1802960‑1803258

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A