Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F142 I0 R1 859 274.6 8723680 96.4% 8409627 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,102,205 C→A 28.0% V287V (GTG→GTT)  wzxB ← putative polisoprenol‑linked O‑antigen transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,102,2050CA28.0% 337.5 / 128.2 170V287V (GTG→GTT) wzxBputative polisoprenol‑linked O‑antigen transporter
Reads supporting (aligned to +/- strand):  ref base C (65/57);  new base A (20/28);  total (85/85)
Fisher's exact test for biased strand distribution p-value = 2.33e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.49e-01

Only 100 of 174 total aligned reads displayed.

AGAGGCGATATAATCATTAGCACAATCACTGCATTATCATATCCCGGCCCTATACTTATTTTTACTAGTATAGATGCACCCAAGAGCAGAATTAATGAAAAAGCACCACCAATCAAACTCAAGCAGGTCAATGATTTTTTAATTAAAATCACACCCTTCACACGATTAAGAACAAGCGTACTTGATATTCTTGGGTATATTGCTTGGGTGATAGGATTTAATAGCCCTTGAAGCGCGTTTCTTATAGTATTGGCCGCATTAAAATTCCCTACGGACGTTGGTCCAGATATAAATCCCAG  >  CP009273/2102056‑2102354
                                                                                                                                                     |                                                                                                                                                     
  agGCGATATAATCATTAGCACAATCACTGCATTATCATATCCCGGCCCTATACTTATTTTTACTAGTATAGATGCACCCAAGAGCAGAATTAATGAAAAAGCACCACCAATCAAACTCAAGCAGGTCAATGATTTTTTAATTAAAATcac                                                                                                                                                     >  1:4006221/1‑150 (MQ=255)
   ggCGATATAATCATTAGCACAATCACTGCATTATCATATCCCGGCCCTATACTTATTTTTACTAGTATAGATGCACCCAAGAGCAGAATTAATGAAAAAGCACCACCAATCAAACTCAAGCAGGTCAATGATTTTTTAATTAAAATcaca                                                                                                                                                    >  2:794751/1‑150 (MQ=255)
      tatatAATCATTATCAAAATCACTGCATTATCATATCCAGTCACTATACTTATTTTTACTATTATAGATGCACCCAAGAGAATAATTAATGAAAAAGCACCACCAATAAAACTCAAGCAGGTCAATGATTTTTTAATTAAAATCACAccc                                                                                                                                                 <  1:1432059/149‑1 (MQ=255)
              cATTAGCACAATCACTGCATTATCATATCCCGGCCCTATACTTATTTTTACTAGTATAGATGCACCCAAGAGCAGAATTAATGAAAAAGCACCACCAATCAAACTCAAGCAGGTCAATGATTTTTTAATTAAAATAACACCCTTCACACg                                                                                                                                         <  2:2860445/150‑1 (MQ=255)
                ttAGCACAATCACTGCATTATCATATCCCGGCCCTATACTTATTTTTACTAGTATAGATGCACCCAAGAGCAGAATTAATGAAAAAGCACCACCAATCAAACTCAAGCAGGTCAATGATTTTTTAATTAAAATCACACCCTTCACACGAt                                                                                                                                       >  2:3558313/1‑150 (MQ=255)
                 tAGCACAATCACTGCATTATCATATCCCGGCCCTATACTTATTTTTACTAGTATAGATGCACCCAAGAGCAGAATTAATGAAAAAGCACCACCAATCAAACTCAAGCAGGTCAATGATTTTTTAATTAAAATCACACCCTTCACACGAtt                                                                                                                                      <  1:370910/150‑1 (MQ=255)
                   gCACAATCACTGCATTATCATATCCCGGCCCTATACTTATTTTTACTAGTATAGATGCACCCAAGAGCAGAATTAATGAAAAAGCACCACCAATCAAACTCAAGCAGGTCAATGATTTTTTAATTAAAATCACACCCTTCACACGATTaa                                                                                                                                    >  2:3490060/1‑150 (MQ=255)
                    cacaATCACTGCATTATCATATCCCGGCCCTATACTTATTTTTACTAGTATAGATGCACCCAAGAGCAGAATTAATGAAAAAGCACCACCAATCAAACTCAAGCAGGTCAATGATTTTTTAATTAAAATCACACCCTTCACACGATTAAg                                                                                                                                   <  2:3313087/150‑1 (MQ=255)
                        aTCACTGCATTATCATATCCCGGCCCTATACTTATTTTTACTAGTATAGATGCACCCAAGAGCAGAATTAATGAAAAAGCACCACCAATCAAACTCAAGCAGGTCAATGATTTTTTAATTAAAATCACACCCTTCACACGATTAAGAACa                                                                                                                               >  1:2572398/1‑150 (MQ=255)
                            cTGCATTATCATATCCCGGCCCTATACTTATTTTTACTAGTATAGATGCACCCAAGAGCAGAATTAATGAAAAAGCACCACCAATCAAACTCAAGCAGGTCAATGATTTTTTAATTAAAATCACACCCTTCACACGATTAAGAACAAGCg                                                                                                                           >  2:1502498/1‑150 (MQ=255)
                             tGCATTATCATATCCCGGCCCTATACTTATTTTTACTAGTATAGATGCACCCAAGAGCAGAATTAATGAAAAAGCACCACCAATCAAACTCAAGCAGGTCAATGATTTTTTAATTAAAATAACACCCTTCACACGATTAAGAACAAGAGt                                                                                                                          >  2:3560210/1‑150 (MQ=255)
                              gCATTATCATATCCCGGCCCTATACTTATTTTTACTAGTATAGATGCACCCAAGAGCAGAATTAATGAAAAAGCACCACCAATCAAACTCAAGCAGGTCAATGATTTTTTAATTAAAATAACACCCTTCACACGATTAAGAACAAGCGTa                                                                                                                         <  2:543817/150‑1 (MQ=255)
                                aTTATCATATCCCGGCCCTATACTTATTTTTACTAGTATAGATGCACCCAAGAGCAGAATTAATGAAAAAGCACCACCAATCAAACTCAAGCAGGTCAATGATTTTTTAATTAAAATAACACCCTTCACACGATTAATAACAAGCGTACt                                                                                                                       >  1:2213287/1‑150 (MQ=255)
                                     cATATCCCGGCCCTATACTTATTTTTACTAGTATAGATGCACCCAAGAGCAGAATTAATGAAAAAGCACCACCAATCAAACTCAAGCAGGTCAATGATTTTTTAATTAAAATCACACCCTTCACACGATTAAGAACAAGCGTACTTGata                                                                                                                  <  2:2805672/150‑1 (MQ=255)
                                        atCCCGGCCCTATACTTATTTTTACTAGTATAGATGCACCCAAGAGCAGAATTAATGAAAAAGCACCACCAATCAAACTCAAGCAGGTCAATGATTTTTTAATTAAAATAACACCCTTCACACGATTAAGAACAAGCGTACTTGATATTc                                                                                                               >  2:456717/1‑150 (MQ=255)
                                         tCCCGGCCCTATACTTATTTTTACTAGTATAGATGCACCCAAGAGCAGAATTAATGAAAAAGCACCACCAATCAAACTCAAGCAGGTCAATGATTTTTTAATTAAAATAACACCCTTCACACGATTAAGAACAAGCGTACTTGATATTCt                                                                                                              <  1:1774536/150‑1 (MQ=255)
                                            cGGCCCTATACTTATTTTTACTAGTATAGATGCACCCAAGAGCAGAATTAATGAAAAAGCACCACCAATCAAACTCAAGCAGGTCAATGATTTTTTAATTAAAATCACACCCTTCACACGATTAAGAACAAGCGTACTTGATATTCTTgg                                                                                                           >  2:1319272/1‑150 (MQ=255)
                                               cccTATACTTATTTTTACTAGTATAGATGCACCCAAGAGCAGAATTAATGAAAAAGCACCACCAATCAAACTCAAGCAGGTCAATGATTTTTTAATTAAAATCACACCCTTCACACGATTAAGAACAAGCGTACTTGATATTCTTGGGta                                                                                                        >  1:3447855/1‑150 (MQ=255)
                                                ccTATACTTATTTTTACTAGTATAGATGCACCCAAGAGCAGAATTAATGAAAAAGCACCACCAATCAAACTCAAGCAGGTCAATGATTTTTTAATTAAAATCACACCCTTCACACGATTAAGAACAAGCGTACTTGATATTCTTGGGtat                                                                                                       <  2:2172040/150‑1 (MQ=255)
                                                  tataCTTATTTTTACTAGTATAGATGCACCCAAGAGCAGAATTAATGAAAAAGCACCACCAATCAAACTCAAGCAGGTCAATGATTTTTTAATTAAAATCACACCCTTCACACGATTAAGAACAAGCGTACTTGATATTCTTGGGTatat                                                                                                     >  2:1250318/1‑150 (MQ=255)
                                                  tataCTTATTTTTACTAGTATAGATGCACCCAAGAGCAGAATTAATGAAAAAGCACCACCAATCAAACTCAAGCAGGTCAATGATTTTTTAATTAAAATCACACCCTTCACACGATTAAGAACAAGCGTACTTGATATTCTTGGGTatat                                                                                                     >  2:541181/1‑150 (MQ=255)
                                                  tataCTTATTTTTACTAGTATAGATGCACCCAAGAGCAGAATTAATGAAAAAGCACCACCAATCAAACTCAAGCAGGTCAATGATTTTTTAATTAAAATAACACCCTTCACACGATTAAGAACAAGCGTACTTGATATTCTTGGGTatat                                                                                                     <  2:1611405/150‑1 (MQ=255)
                                                    taCTTATTTTTACTAGTATAGATGCACCCAAGAGCAGAATTAATGAAAAAGCACCACCAATCAAACTCAAGCAGGTCAATGATTTTTTAATTAAAATCACACCCTTCACACGATTAAGAACAAGCGTACTTGATATTCTTGGGTATATTg                                                                                                   >  1:1462342/1‑150 (MQ=255)
                                                     aCTTATTTTTACTAGTATAGATGCACCCAAGAGCAGAATTAATGAAAAAGCACCACCAATCAAACTCAAGCAGGTCAATGATTTTTTAATTAAAATCACACCCTTCACACGATTAAGAACAAGCGTACTTGATATTCTTGGGTATATTGc                                                                                                  >  1:2382220/1‑150 (MQ=255)
                                                         ctttttACTAGTATAGATGCACCCAAGAGCAGAATTAATGAAAAAGCACCACCAATCACACTCAAGCAGGTCACTGATTTTTTAATTAAAATCACACCCTTCACACGATTAAGAACAAGCGTACTTGATATTCTTGGGTATATTGCTTgg                                                                                              <  2:626717/149‑1 (MQ=255)
                                                          tttttACTAGTATAGATGCACCCAAGAGCAGAATTAATGAAAAAGCACCACCAATCAAACTCAAGCAGGTCAATGATTTTTTAATTAAAATAACACCCTTCACACGATTAAGAACAAGCGTACTTGATATTCTTGGGTATATTGCTTggg                                                                                             >  2:1686891/1‑150 (MQ=255)
                                                            tttACTAGTATAGATGCACCCAAGAGCAGAATTAATGAAAAAGCACCACCAATCAAACTCAAGCAGGTCAATGATTTTTTAATTAAAATCACACCCTTCACACGATTAAGAACAAGCGTACTTGATATTCTTGGGTATATTGCTTGGGTg                                                                                           <  1:2802540/150‑1 (MQ=255)
                                                            tttACTAGTATAGATGCACCCAAGAGCAGAATTAATGAAAAAGCACCACCAATCAAACTCAAGCAGGTCAATGATTTTTTAATTAAAATCACACCCTTCACACGATTAAGAACAAGCGTACTTGATATTCTTGGGTATATTGCTTGGGTg                                                                                           <  1:2802517/150‑1 (MQ=255)
                                                                cTAGTATAGATGCACCCAAGAGCAGAATTAATGAAAAAGCACCACCAATCAAACTCAAGCAGGTCAATGATTTTTTAATTAAAATCACACCCTTCACACGATTAAGAACAAGCGTACTTGATATTCTTGGGTATATTGCTTGGGTGATAg                                                                                       <  1:3689531/150‑1 (MQ=255)
                                                                cTAGTATAGATGCACCCAAGAGCAGAATTAATGAAAAAGCACCACCAATCAAACTCAAGCAGGTCAATGATTTTTTAATTAAAATCACACCCTTCACACGATTAAGAACAAGCGTACTTGATATTCTTGGGTATATTGCTTGGGTGATAg                                                                                       >  1:1852037/1‑150 (MQ=255)
                                                                 tAGTATAGATGCACCCAAGAGCAGAATTAATGAAAAAGCACCACCAATCAAACTCAAGCAGGTCAATGATTTTTTAATTAAAATCACACCCTTCACACGATTAAGAACAAGCGTACTTGATATTCTTGGGTATATTGCTTGGGTGATAgg                                                                                      >  2:200456/1‑150 (MQ=255)
                                                                  aGTATAGATGCACCCAAGAGCAGAATTAATGAAAAAGCACCACCAATCAAACTCAAGCAGGTCAATGATTTTTTAATTAAAATCACACCCTTCACACGATTAAGAACAAGCGTACTTGATATTCTTGGGTATATTGCTTGGGTGATAGGa                                                                                     >  2:1869596/1‑150 (MQ=255)
                                                                   gTATAGATGCACCCAAGAGCAGAATTAATGAAAAAGCACCACCAATCAAACTCAAGCAGGTCAATGATTTTTTAATTAAAATCACACCCTTCACACGATTAAGAACAAGCGTACTTGATATTCTTGGGTATATTGCTTGGGTGATAGGAt                                                                                    <  2:418987/150‑1 (MQ=255)
                                                                    tataGATGCACCCAAGAGCAGAATTAATGAAAAAGCACCACCAATCAAACTCAAGCAGGTCAATGATTTTTTAATTAAAATCACACCCTTCACACGATTAAGAACAAGCGTACTTGATATTCTTGGGTATATTGCTTGGGTGATAGGAtt                                                                                   >  2:488116/1‑150 (MQ=255)
                                                                     ataGATGCACCCAAGAGCAGAATTAATGAAAAAGCACCACCAATCAAACTCAAGCAGGTCAATGATTTTTTAATTAAAATCACACCCTTCACACGATTAAGAACAAGCGTACTTGATATTCTTGGGTATATTGCTTGGGTGATAGGAttt                                                                                  >  1:2679819/1‑150 (MQ=255)
                                                                     ataGATGCACCCAAGAGCAGAATTAATGAAAAAGCACCACCAATCAAACTCAAGCAGGTCAATGATTTTTTAATTAAAATAACACCCTTCACACGATTAAGAACAAGCGTACTTGATATTCTTGGGTATATTGCTTGGGTGATAGGAttt                                                                                  <  2:3170890/150‑1 (MQ=255)
                                                                       aGATGCACCCAAGAGCAGAATTAATGAAAAAGCACCACCAATCAAACTCAAGCAGGTCAATGATTTTTTAATTAAAATCACACCCTTCACACGATTAAGAACAAGCGTACTTGATATTCTTGGGTATATTGCTTGGGTGATAGGATTTaa                                                                                <  2:2393513/150‑1 (MQ=255)
                                                                        gATGCACCCAAGAGCAGAATTAATGAAAAAGCACCACCAATCAAACTCAAGCAGGTCAATGATTTTTTAATTAAAATAACACCCTTCACACGATTAAGAACAAGCGTACTTGATATTCTTGGGTATATTGCTTGGGTGATAGGATTTAAt                                                                               <  1:3561068/150‑1 (MQ=255)
                                                                        gATGCACCCAAGAGCAGAATTAATGAAAAAGCACCACCAAGCAAACTCAAGCAGGTCAATGATTTTTTAATTAAAATAACACCCTTCACACGATTAAGAACAAGCGTACTTGATATTCTTGGGTATATTGCTTGGGTGATAGGATTTAAt                                                                               >  1:3080986/1‑150 (MQ=255)
                                                                         aTGCACCCAAGAGCAGAATTAATGAAAAAGCACCACCAATCAAACTCAAGCAGGTCAATGATTTTTTAATTAAAATCACACCCTTCACACGATTAAGAACAAGCGTACTTGATATTCTTGGGTATATTGCTTGGGTGATAGGATTTAATa                                                                              >  2:1057092/1‑150 (MQ=255)
                                                                         aTGCACCCAAGAGCAGAATTAATGAAAAAGCACCACCAATCAAACTCAAGCAGGTCAATGATTTTTTAATTAAAATCACACCCTTCACACGATTAAGAACAAGCGTACTTGATATTCTTGGGTATATTGCTTGGGTGATAGGATTTAATa                                                                              >  2:1150147/1‑150 (MQ=255)
                                                                           gCNCCCAAGAGCAGAATTAATGAAAAAGCACCACCAATCAAACTCAAGCAGGTCAATGATTTTTTAATTAAAATCACACCCTTCACACGATTAAGAACAAGCGTACTTGATATTCTTGGGTATATTGCTTGGGTGATAGGATTTAATAGc                                                                            >  2:1032567/1‑150 (MQ=255)
                                                                           gCACCCAAGATCAGAATTAATGAAAAAGCACCACCACTCAAACTCAAGCAGGTCAATGATTTTTTAATTAAAATAACACCCTTCACACGATTAAGAACAAGCGTACTTGATATTCTTGGGTATATTGCTTGGGTGATAGGATTTAATAGc                                                                            <  2:3903931/150‑1 (MQ=255)
                                                                           gCACCCAAGAGCAGAATTAATGAAAAAGCACCACCAATCAAACTCAAGCAGGTCAATGATTTTTTAATTAAAATAACACCCTTCACACGATTAAGAACAAGCGTACTTGATATTCTTGGGTATATTGCTTGGGTGATAGGATTTAATAGc                                                                            <  2:1717639/150‑1 (MQ=255)
                                                                           gCACCCAAGAGCAGAATTAATGAAAAAGCACCACCAATCAAACTCAAGCAGGTCAATGATTTTTTAATTAAAATAACACCCTTCACACGATTAAGAACAAGCGTACTTGATATTCTTGGGTATATTGCTTGGGTGATAGGATTTAATAGc                                                                            <  2:3837922/150‑1 (MQ=255)
                                                                            cACCCAAGAGCAGAATTAATGAAAAAGCACCACCAATCAAACTCAAGCAGGTCAATGATTTTTTAATTAAAATCACACCCTTCACACGATTAAGAACAAGCGTACTTGATATTCTTGGGTATATTGCTTGGGTGATAGGATTTAATAGcc                                                                           >  2:3035079/1‑150 (MQ=255)
                                                                            cACCCAAGAGCAGAATTAATGAAAAAGCACCACCAATCAAACTCAAGCAGGTCAATGATTTTTTAATTAAAATCACACCCTTCACACGATTAAGAACAAGCGTACTTGATATTCTTGGGTATATTGCTTGGGTGATAGGATTTAATAGcc                                                                           >  2:856214/1‑150 (MQ=255)
                                                                            cACCCAAGAGCAGAATTAATGAAAAAGCACCACCAATCAAACTCAAGCAGGTCAATGATTTTTTAATTAAAATCACACCCTTCACACGATTAAGAACAAGCGTACTTGATATTCTTGGGTATATTGCTTGGGTGATAGGATTTAATAGcc                                                                           <  1:50661/150‑1 (MQ=255)
                                                                               ccAAGAGCAGAATTAATGAAAAAGCACCACCAATCAAACTCAAGCAGGTCAATGATTTTTTAATTAAAATCACACCCTTCACACGATTAAGAACAAGCGTACTTGATATTCTTGGGTATATTGCTTGGGTGATAGGATTTAATAGCCCtt                                                                        <  2:3239394/150‑1 (MQ=255)
                                                                               ccAAGAGCAGAATTAATGAAAAAGCACCACCAATCAAACTCAAGCAGGTCAATGATTTTTTAATTAAAATAACACCCTTCACACGATTAAGAACAAGCGTACTTGATATTCTTGGGTATATTGCTTGGGTGATAGGATTTAATAGCCCtt                                                                        >  1:3736325/1‑150 (MQ=255)
                                                                                cAAGAGCAGAATTAATGAAAAAGCACCACCAATCAAACTCAAGCAGGTCAATGATTTTTTAATTAAAATAACACCCTTCACACGATTAAGAACAAGCGTACTTGATATTCTTGGGTCTATTGCTTGGGTGATAGGATTTAATAGCCCTTg                                                                       >  2:2917453/1‑150 (MQ=255)
                                                                                 aaGAGCAGAATTAATGAAAAAGCACCACCAATCAAACTCAAGCAGGTCAATGATTTTTTAATTAAAATCACACCCTTCACACGATTAAGAACAAGCGTACTTGATATTCTTGGGTATATTGCTTGGGTGATAGGATTTAATAGCCCTTGa                                                                      >  2:3334269/1‑150 (MQ=255)
                                                                                  agagCAGAATTAATGAAAAAGCACCACCAATCAAACTCAAGCAGGTCAATGATTTTTTAATTAAAATCACACCCTTCACACGATTAAGAACAAGCGTACTTGATATTCTTGGGTATATTGCTTGGGTGATAGGATTTAATAGCCCTTGaa                                                                     <  1:453176/150‑1 (MQ=255)
                                                                                  agagCAGAATTAATGAAAAAGCACCACCAATCAAACTCAAGCAGGTCAATGATTTTTTAATTAAAATCACACCCTTCACACGATTAAGAACAAGCGTACTTGATATTCTTGGGTATATTGCTTGGGTGATAGGATTTAATAGCCCTTGaa                                                                     <  1:453195/150‑1 (MQ=255)
                                                                                   gagCAGAATTAATGAAAAAGCACCACCAATCAAACTCAAGCAGGTCAATGATTTTTTAATTAAAATCACACCCTTCACACGATTAAGAACAAGCGTACTTGATATTCTTGGGTATATTGCTTGGGTGATAGGATTTAATAGCCCTTGAAg                                                                    <  2:3585747/150‑1 (MQ=255)
                                                                                   gagCAGAATTAATGAAAAAGCACCACCAATCAAACTCAAGCAGGTCAATGATTTTTTAATTAAAATCACACCCTTCACACGATTAAGAACAAGCGTACTTGATATTCTTGGGTATATTGCTTGGGTGATAGGATTTAATAGCCCTTGAAg                                                                    >  2:3146459/1‑150 (MQ=255)
                                                                                     gCAGAATTAATGAAAAAGCACCACCAATCAAACTCAAGCAGGTCAATGATTTTTTAATTAAAATAACACCCTTCACACGATTAAGAACAAGCGTACTTGATATTCTTGGGTATATTGCTTGGGTGATAGGATTTAATAGCCCTTGAAgcg                                                                  <  2:1228465/150‑1 (MQ=255)
                                                                                         aaTTAATGAAAAAGCACCACCAATCAAACTCAAGCAGGTCAATGATTTTTTAATTAAAATCACACCCTTCACACGATTAAGAACAAGCGTACTTGATATTCTTGGGTATATTGCTTGGGTGATAGGATTTAATAGCCCTTGAAGCGCGtt                                                              >  1:3902495/1‑150 (MQ=255)
                                                                                         aaTTAATGAAAAAGCACCACCAATCAAACTCAAGCAGGTCAATGATTTTTTAATTAAAATAACACCCTTCACACGATTAAGAACAAGCGTACTTGATATTCTTGGGTATATTGCTTGTGTGATAGGATTTAATAGCCCTTGAAGCGCGtt                                                              >  1:1843536/1‑150 (MQ=255)
                                                                                         aaTTAATGAAAAAGCACCACCAATCAAACTCAAGCAGGTCAATGATTTTTTAATTAAAATAACACCCTTCACACGATTAAGAACAAGCGTACTTGATATTCTTGGGTATATTGCTTGGGTGATAGGATTTAATAGCCCTTGAAGCGCGtt                                                              <  1:1302212/150‑1 (MQ=255)
                                                                                          aTTAATGAAAAAGCACCACCAATCAAACTCAAGCAGGTCAATGATTTTTTAATTAAAATCACACCCTTCACACGATTAAGAACAAGCGTACTTGATATTCTTGGGTATATTGCTTGTGTGATAGGATTTAATATCCCTTGAAGCGCGttt                                                             >  1:3754397/1‑150 (MQ=255)
                                                                                          aTTAATGAAAAAGCACCACCAATCAAACTCAAGCAGGTCAATGATTTTTTAATTAAAATAACACCCTTCACACGATTAAGAACAAGCGTACTTGATATTCTTGGGTATATTGCTTGGGTGATAGGATTTAATAGCCCTTGAAGCGCGttt                                                             >  1:4139488/1‑150 (MQ=255)
                                                                                          aTTAATGAAAAAGCACCACCAATCAAACTCAAGCAGGTCAATGATTTTTTAATTAAAATAACACCCTTCACACGATTAAGAACAAGCGTACTTGATATTCTTGGGTATATTGCTTGGGTGATAGGATTTAATAGCCCTTGAAGCGCGttt                                                             >  1:1673285/1‑150 (MQ=255)
                                                                                               tGAAAAAGCACCACCAATCAAACTCAAGCAGGTCAATGATTTTTTAATTAAAATCACACCCTTCACACGATTAAGAACAAGCGTACTTGATATTCTTGGGTATATTGCTTGGGTGATAGGATTTAATAGCCCTTGAAGCGCGTTTCTtat                                                        >  1:1011844/1‑150 (MQ=255)
                                                                                                    aaGCACCACCAATCAAACTCAAGCAGGTCAATGATTTTTTAATTAAAATCACACCCTTCACACGATTAAGAACAAGCGTACTTGATATTCTTGGGTATATTGCTTGGGTGATAGGATTTAATAGCCCTTGAAGCGCGTTTCTTATAGTAt                                                   >  1:4063524/1‑150 (MQ=255)
                                                                                                     aGCACCACCAATCAAACTCAAGCAGGTCAATGATTTTTTAATTAAAATCACACCCTTCACACGATTAAGAACAAGCGTACTTGATATTCTTGGGTATATTGCTTGGGTGATAGGATTTAATAGCCCTTGAAGCGCGTTTCTTATAGTAtt                                                  <  1:828817/150‑1 (MQ=255)
                                                                                                      gCACCACCAATCAAACTCAAGCAGGTCAATGATTTTTTAATTAAAATAACACCCTTCACACGATTAAGAACAAGCGTACTTGATATTCTTGGGTATATTGCTTGGGTGATAGGATTTAATAGCCCTTGAAGCGCGTTTCTTATAGTATTg                                                 <  1:2192468/150‑1 (MQ=255)
                                                                                                       caccacCAATCAAACTCAAGCAGGTCAATGATTTTTTAATTAAAATCACACCCTTCACACGATTAAGAACAAGCGTACTTGATATTCTTGGGTATATTGCTTGGGTGATAGGATTTAATAGCCCTTGAAGCGCGTTTCTTATAGTATTgg                                                >  1:4021790/1‑150 (MQ=255)
                                                                                                          caccaATCAAACTCAAGCAGGTCAATGATTTTTTAATTAAAATAACACCCTTCACACGATTAAGAACAAGCGTACTTGATATTCTTGGGTATATTGCTTGGGTGATAGGATTTAATAGCCCTTGAAGCGCGTTTCTTATAGTATTGGCCg                                             <  1:102476/150‑1 (MQ=255)
                                                                                                            ccaATCAAACTCAAGCAGGTCAATGATTTTTTAATTAAAATCACACCCTTCACACGATTAAGAACAAGCGTACTTGATATTCTTGGGTATATTGCTTGGGTGATAGGATTTAATAGCCCTTGAAGCGCGTTTCTTATAGTATTGGCCGCa                                           <  2:1747965/150‑1 (MQ=255)
                                                                                                            ccaATCAAACTCAAGCAGGTCAATGATTTTTTAATTAAAATCACACCCTTCACACGATTAAGAACAAGCGTACTTGATATTCTTGGGTATATTGCTTGGGTGATAGGATTTAATAGCCCTTGAAGCGCGTTTCTTATAGTATTGGCCGCa                                           >  1:3920276/1‑150 (MQ=255)
                                                                                                             caATCAAACTCAAGCAGGTCAATGATTTTTTAATTAAAATAACACCCTTCACACGATTAAGAACAAGCGTACTTGATATTCTTGGGTATATTGCTTGGGTGATAGGATTTAATAGCCCTTGAAGCGCGTTTCTTATAGTATTGGCCGCAt                                          <  2:1511911/150‑1 (MQ=255)
                                                                                                              aaTCAAACTCAAGCAGGTCAATGATTTTTTAATTAAAATCACACCCTTCACACGATTAAGAACAAGCGTACTTGATATTCTTGGGTATATTGCTTGGGTGATAGGATTTAATAGCCCTTGAAGCGCGTTTCTTATAGTATTGGCCGCAtt                                         >  2:3999922/1‑150 (MQ=255)
                                                                                                              aaTCAAACTCAAGCAGGTCAATGATTTTTTAATTAAAATCACACCCTTCACACGATTAAGAACAAGCGTACTTGATATTCTTGGGTATATTGCTTGGGTGATAGGATTTAATAGCCCTTGAAGCGCGTTTCTTATAGTATTGGCCGCAtt                                         >  2:3999852/1‑150 (MQ=255)
                                                                                                                 cAAACTCAAGCAGGTCAATGATTTTTTAATTAAAATCACACCCTTCACACGATTAAGAACAAGCGTACTTGATATTCTTGGGTATATTGCTTGGGTGATAGGATTTAATAGCCCTTGAAGCGCGTTTCTTATAGTATTGGCCGCATTaaa                                      >  1:2865458/1‑150 (MQ=255)
                                                                                                                 cAAACTCAAGCAGGTCAATGATTTTTTAATTAAAATCACACCCTTCACACGATTAAGAACAAGCGTACTTGATATTCTTGGGTATATTGCTTGGGTGATAGGATTTAATAGCCCTTGAAGCGCGTTTCTTATAGTATTGGCCGCATTaaa                                      >  1:1088267/1‑150 (MQ=255)
                                                                                                                 cAAACTCAAGCAGGTCAATGATTTTTTAATTAAAATAACACCCTTCACACGATTAAGAACAAGCGTACTTGATATTCTTGGGTATATTGCTTGGGTGATAGGATTTAATAGCCCTTGAAGCGCGTTTCTTATAGTATTGGCCGCATTaaa                                      >  2:526227/1‑150 (MQ=255)
                                                                                                                   aaCTCAAGCAGGTCAATGATTTTTTAATTAAAATCACACCCTTCACACGATTAAGAACAAGCGTACTTGATATTCTTGGGTATATTGCTTGGGTGATAGGATTTAATAGCCCTTGAAGCGCGTTTCTTATAGTATTGGCCGCATTAAAAt                                    >  1:1555509/1‑150 (MQ=255)
                                                                                                                   aaCTCAAGCAGGTCAATGATTTTTTAATTAAAATAACACCCTTCACACGATTAAGAACAAGCGTACTTGATATTCTTGGGTATATTGCTTGGGTGATAGGATTTAATAGCCCTTGAAGCGCGTTTCTTATAGTATTGGCCGCATTAAAAt                                    <  2:4159779/150‑1 (MQ=255)
                                                                                                                    aCTCAAGCAGGTCAATGATTTTTTAATTAAAATCACACCCTTCACACGATTAAGAACAAGCGTACTTGATATTCTTGGGTATATTGCTTGGGTGATAGGATTTAATAGCCCTTGAAGCGCGTTTCTTATAGTATTGGCCGCATTAAAAtt                                   >  2:3439379/1‑150 (MQ=255)
                                                                                                                         aGCAGGTCAATGATTTTTTAATTAAAATCACACCCTTCACACGATTAAGAACAAGCGTACTTGATATTCTTGGGTATATTGCTTGGGTGATAGGATTTAATAGCCCTTGAAGCGCGTTTCTTATAGTATTGGCCGCATTAAAATTCCCTa                              <  1:3600080/150‑1 (MQ=255)
                                                                                                                          gCAGGTCAATGATTTTTTAATTAAAATCACACCCTTCACACGATTAAGAACAAGCGTACTTGATATTCTTGGGTATATTGCTTGGGTGATAGGATTTAATAGCCCTTGAAGCGCGTTTCTTATAGTATTGGCCGCATTAAAATTCCCTAc                             <  1:2427116/150‑1 (MQ=255)
                                                                                                                              gTCAATGATTTTTTAATTAAAATCACACCCTTCACACGATTAAGAACAAGCGTACTTGATATTCTTGGGTATATTGCTTGGGTGATAGGATTTAATAGCCCTTGAAGCGCGTTTCTTATAGTATTGGCCGCATTAAAATTCCCTACGGAc                         >  2:2432310/1‑150 (MQ=255)
                                                                                                                              gTCAATGATTTTTTAATTAAAATCACACCCTTCACACGATTAAGAACAAGCGTACTTGATATTCTTGGGTATATTGCTTGGGTGATAGGATTTAATAGCCCTTGAAGCGCGTTTCTTATAGTATTGGCCGCATTAAAATTCCCTACGGAc                         <  2:1363058/150‑1 (MQ=255)
                                                                                                                              gTCAATGATTTTTTAATTAAAATCACACCCTTCACACGATTAAGAACAAGCGTACTTGATATTCTTGGGTATATTGCTTGGGTGATAGGATTTAATAGCCCTTGAAGCGCGTTTCTTATAGTATTGGCCGCATTAAAATTCCCTACGGAc                         <  2:3102029/150‑1 (MQ=255)
                                                                                                                               tCAATGATTTTTTAATTAAAATCACACCCTTCACACGATTAAGAACAAGCGTACTTGATATTCTTGGGTATATTGCTTGGGTGATAGGATTTAATAGCCCTTGAAGCGCGTTTCTTATAGTATTGGCCGCATTAAAATTCCCTACGGACg                        <  2:2572398/150‑1 (MQ=255)
                                                                                                                                      ttttttAATTAAAATCACACCCTTCACACGATTAAGAACAAGCGTACTTGATATTCTTGGGTATATTGCTTGGGTGATAGGATTTAATAGCCCTTGAAGCGCGTTTCTTATAGTATTGGCCGCATTAAAATTCCCTACGGACGTTGGTcc                 <  2:1078757/150‑1 (MQ=255)
                                                                                                                                       tttttAATTAAAATCACACCCTTCACACGATTAAGAACAAGCGTACTTGATATTCTTGGGTATATTGCTTGGGTGATAGGATTTAATAGCCCTTGAAGCGCGTTTCTTATAGTATTGGCCGCATTAAAATTCCCTACGGACGTTGGTCCa                >  2:3827530/1‑150 (MQ=255)
                                                                                                                                       tttttAATTAAAATCACACCCTTCACACGATTAAGAACAAGCGTACTTGATATTCTTGGGTATATTGCTTGGGTGATAGGATTTAATAGCCCTTGAAGCGCGTTTCTTATAGTATTGGCCGCATTAAAATTCCCTACGGACGTTGGTCCa                <  1:1218923/150‑1 (MQ=255)
                                                                                                                                          ttaattaaAATCACACCCTTCACACGATTAAGAACAAGCGTACTTGATATTCTTGGGTATATTGCTTGGGTGATAGGATTTAATAGCCCTTGAAGCGCGTTTCTTATAGTATTGGCCGCATTAAAATTCCCTACGGACGTTGGTCCAGat             >  2:2000017/1‑150 (MQ=255)
                                                                                                                                          ttaattaaAATCACACCCTTCACACGATTAAGAACAAGCGTACTTGATATTCTTGGGTATATTGCTTGGGTGATAGGATTTAATAGCCCTTGAAGCGCGTTTCTTATAGTATTGGCCGCATTAAAATTCCCTACGGACGTTGGTCCAGat             >  1:1120832/1‑150 (MQ=255)
                                                                                                                                           taattaaAATCACACCCTTCACACGATTAAGAACAAGCGTACTTGATATTCTTGGGTATATTGCTTGGGTGATAGGATTTAATAGCCCTTGAAGCGCGTTTCTTATAGTATTGGCCGCATTAAAATTCCCTACGGACGTTGGTCCAGata            <  1:1869619/150‑1 (MQ=255)
                                                                                                                                           taattaaAATAACACCCTTCACACGATTAAGAACAAGCGTACTTGATATTCTTGGGTATATTGCTTGGGTGATAGGATTTAATAGCCCTTGAAGCGCGTTTCTTATAGTATTGGCCGCATTAAAATTCCCTACGGACGTTGGTCCAGata            <  1:681222/150‑1 (MQ=255)
                                                                                                                                            aattaaAATCACACCCTTCACACGATTAAGAACAAGCGTACTTGATATTCTTGGGTATATTGCTTGGGTGATAGGATTTAATAGCCCTTGAAGCGCGTTTCTTATAGTATTGGCCGCATTAAAATTCCCTACGGACGTTGGTCCAGatat           <  1:3184284/150‑1 (MQ=255)
                                                                                                                                               taaAATCACACCCTTCACACGATTAAGAACAAGCGTACTTGATATTCTTGGGTATATTGCTTGGGTGATAGGATTTAATAGCCCTTGAAGCGCGTTTCTTATAGTATTGGCCGCATTAAAATTCCCTACGGACGTTGGTCCAGATATaaa        >  2:3966248/1‑150 (MQ=255)
                                                                                                                                                aaaaTAACACCCTTCACACGATTAAGAACAAGCGTACTTGATATTCTTGGGTATATTGCTTGGGTGATAGGATTTAATAGCCCTTGAAGCGCGTTTCTTATAGTATTGGCCGCATTAAAATTCCCTACGGACGTTGGTCCAGATATAAAt       <  2:3736325/150‑1 (MQ=255)
                                                                                                                                                 aaaTCACACCCTTCACACGATTAAGAACAAGCGTACTTGATATTCTTGGGTATAGTGCTTGGGTGATAGGATTTAATAGCCCTTGAAGCGCGTTTCTTATAGTATTGGCCGCATTAAAATTCCCTACGGACGTTGGTCCAGATATAAATc      <  2:1540321/150‑1 (MQ=255)
                                                                                                                                                 aaaTAACACCCTTCACACGATTAAGAACAAGCGTACTTGATATTCTTGGGTATATTGCTTGGGTGATAGGATTTAATAGCCCTTGAAGCGCGTTTCTTATAGTATTGGCCGCATTAAAATTCCCTACGGACGTTGGTCCAGATATAAATc      <  1:850961/150‑1 (MQ=255)
                                                                                                                                                   aTCACACCCTTCACACGATTAAGAACAAGCGTACTTGATATTCTTGGGTATATTGCTTGGGTGATAGGATTTAATAGCCCTTGAAGCGCGTTTCTTATAGTATTGGCCGCATTAAAATTCCCTACGGACGTTGGTCCAGATATAAATccc    >  2:2414053/1‑150 (MQ=255)
                                                                                                                                                    tCACACCCTTCACACGATTAAGAACAAGCGTACTTGATATTCTTGGGTATATTGCTTGGGTGATAGGATTTAATAGCCCTTGAAGCGCGTTTCTTATAGTATTGGCCGCATTAAAATTCCCTACGGACGTTGGTCCAGATATAAATCCCa   <  2:2212953/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
AGAGGCGATATAATCATTAGCACAATCACTGCATTATCATATCCCGGCCCTATACTTATTTTTACTAGTATAGATGCACCCAAGAGCAGAATTAATGAAAAAGCACCACCAATCAAACTCAAGCAGGTCAATGATTTTTTAATTAAAATCACACCCTTCACACGATTAAGAACAAGCGTACTTGATATTCTTGGGTATATTGCTTGGGTGATAGGATTTAATAGCCCTTGAAGCGCGTTTCTTATAGTATTGGCCGCATTAAAATTCCCTACGGACGTTGGTCCAGATATAAATCCCAG  >  CP009273/2102056‑2102354

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A