Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F142 I0 R1 859 274.6 8723680 96.4% 8409627 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,225,260 C→T 20.4% intergenic (+67/‑63) yohK → / → cdd LrgB family inner membrane protein/cytidine/deoxycytidine deaminase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,225,2600CT20.4% 608.8 / 142.4 250intergenic (+67/‑63)yohK/cddLrgB family inner membrane protein/cytidine/deoxycytidine deaminase
Reads supporting (aligned to +/- strand):  ref base C (104/94);  new base T (27/24);  total (132/118)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 257 total aligned reads displayed.

CGCTAGCGCTGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGCTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCATTACATGATTATGAGGCAACGCCATGCATCCACGTTTTCAAACCGCTTTTGCCCAACTTGCGGATAACTTGCAATCTGCACTGGAACCTATTCTGGCAGACAAGTACTT  >  CP009273/2225111‑2225408
                                                                                                                                                     |                                                                                                                                                    
 gCTAGCGCTGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATca                                                                                                                                                     >  2:607103/1‑150 (MQ=255)
 gCTAGCGCTGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATca                                                                                                                                                     >  2:2595366/1‑150 (MQ=255)
           gtgtTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGCTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGc                                                                                                                                           <  2:2797645/150‑1 (MQ=255)
             gtTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCca                                                                                                                                         >  1:3100070/1‑150 (MQ=255)
             gtTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCca                                                                                                                                         <  1:160465/150‑1 (MQ=255)
                    gggATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACggg                                                                                                                                  <  2:13064/150‑1 (MQ=255)
                    gggATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACggg                                                                                                                                  >  1:2336232/1‑150 (MQ=255)
                     ggATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACGGGt                                                                                                                                 >  2:890884/1‑150 (MQ=255)
                     ggATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACGGGt                                                                                                                                 >  1:3497971/1‑150 (MQ=255)
                      gATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATTACATATAAAGCCACAACGGGtt                                                                                                                                <  2:377691/150‑1 (MQ=255)
                      gATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACGGGtt                                                                                                                                >  1:739752/1‑150 (MQ=255)
                       aTAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATTACATATAAAGCCACAACGGGTTc                                                                                                                               <  1:1927873/150‑1 (MQ=255)
                       aTAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACGGGTTc                                                                                                                               >  1:686424/1‑150 (MQ=255)
                        tAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAAATAATGAGATTCAGATCACATATAAAGCCACAACGGGTTCg                                                                                                                              >  2:2180993/1‑150 (MQ=255)
                         aaTTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACGGGTTCGt                                                                                                                             >  1:1910089/1‑150 (MQ=255)
                          aTTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACGGGTTCGTa                                                                                                                            >  2:710677/1‑150 (MQ=255)
                              cTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACGGGTTCGTAAACt                                                                                                                        <  1:576418/150‑1 (MQ=255)
                              cTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACGGGTTCGTAAACt                                                                                                                        <  1:2577466/150‑1 (MQ=255)
                               ttCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACGGGTTCGTAAACTg                                                                                                                       >  1:2847442/1‑150 (MQ=255)
                                     gATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACGGGTTCGTAAACTGTTATcc                                                                                                                 >  1:3771800/1‑150 (MQ=255)
                                       tCGCACCGTTCCTTGTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCa                                                                                                               <  1:4200053/150‑1 (MQ=255)
                                        cGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGGTGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCAt                                                                                                              >  1:2705931/1‑150 (MQ=255)
                                        cGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCAt                                                                                                              >  1:2807534/1‑150 (MQ=255)
                                          cACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATTACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCATTa                                                                                                            >  2:2560598/1‑150 (MQ=255)
                                          cACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATTACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCATTa                                                                                                            >  2:4297240/1‑150 (MQ=255)
                                           aCCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCATTAc                                                                                                           >  1:2976584/1‑150 (MQ=255)
                                             cGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCATTACAt                                                                                                         <  2:1718087/150‑1 (MQ=255)
                                              gTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCATTACATg                                                                                                        <  2:1515612/150‑1 (MQ=255)
                                               ttCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATTACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCATTACATGa                                                                                                       <  1:765297/150‑1 (MQ=255)
                                               ttCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATTACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCATTACATGa                                                                                                       <  2:3202207/150‑1 (MQ=255)
                                                tCCTTGTCCCGATTATTCGGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGCTCACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCATTACATGAt                                                                                                      <  2:3913772/150‑1 (MQ=255)
                                                 ccTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCATTACATGAtt                                                                                                     >  2:1205410/1‑150 (MQ=255)
                                                 ccTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCATTACATGAtt                                                                                                     >  2:3397621/1‑150 (MQ=255)
                                                  cTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCATTACATGATTa                                                                                                    <  2:2741015/150‑1 (MQ=255)
                                                      tCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCATTACATGATTATGAg                                                                                                >  1:4209646/1‑150 (MQ=255)
                                                      tCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCATTACATGATTATGAg                                                                                                <  1:4167345/150‑1 (MQ=255)
                                                      tCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCATTACATGATTATGAg                                                                                                <  1:1976941/150‑1 (MQ=255)
                                                        ccGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCATTACATGATTATGAGGc                                                                                              >  2:1511915/1‑150 (MQ=255)
                                                         cGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCATTACATGATTATGAGGCa                                                                                             <  1:342150/150‑1 (MQ=255)
                                                              attCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCATTACATGATTATGAGGCAACGcc                                                                                        >  1:1942012/1‑150 (MQ=255)
                                                                tCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCATTACATGATTATGAGGCAACGCCAt                                                                                      <  2:2278796/150‑1 (MQ=255)
                                                                   ggCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATTACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCATTACATGATTATGAGGCAACGCCATGCa                                                                                   >  1:2910462/1‑150 (MQ=255)
                                                                      aGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATTACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCATTACATGATTATGAGGCAACGCCATGCATcc                                                                                >  2:2236421/1‑150 (MQ=255)
                                                                       gTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATTACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCATTACATGATTATGAGGCAACGCCATGCATCCa                                                                               <  1:3070858/150‑1 (MQ=255)
                                                                         aaTGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCATTACATGATTATGAGGCAACGCCATGCATCCACg                                                                             <  1:784877/150‑1 (MQ=255)
                                                                               ttAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCATTACATGATTATGAGGCAACGCCATGCATCCACGTTTTCa                                                                       <  1:1059491/149‑1 (MQ=255)
                                                                                  aaaTTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCATGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCATTACATGATTATGAGGCAACGCCATGCATCCACGTTTTCAAAc                                                                    >  1:1583857/1‑150 (MQ=255)
                                                                                   aaTTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCATTACATGATTATGAGGCAACGCCATGCATCCACGTTTTCAAAcc                                                                   >  2:82264/1‑150 (MQ=255)
                                                                                      ttGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCATTACATGATTATGAGGCAACGCCATGCATCCACGTTTTCAAACCGCt                                                                <  2:2282099/150‑1 (MQ=255)
                                                                                      ttGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCATTACATGATTATGAGGCAACGCCATGCATCCACGTTTTCAAACCGCt                                                                <  1:2441776/150‑1 (MQ=255)
                                                                                      ttGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCATTACATGATTATGAGGCAACGCCATGCATCCACGTTTTCAAACCGCt                                                                <  1:2441808/150‑1 (MQ=255)
                                                                                        gCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCATTACATGATTATGCGGCAACGCCATGCATCCACGTTTTCAAACCGCttt                                                              <  1:906524/150‑1 (MQ=255)
                                                                                         cGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCATTACATGATTATGAGGCAACGCCATGCATCCACGTTTTCAAACCGCtttt                                                             >  1:1359991/1‑150 (MQ=255)
                                                                                         cGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCATTACATGATTATGAGGCAACGCCATGCATCCACGTTTTCAAACCGCtttt                                                             <  2:2868101/150‑1 (MQ=255)
                                                                                           aTGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATTACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCATTACATGATTATGAGGCAACGCCATGCATCCACGTTTTCAAACCGCTTTTGc                                                           >  2:2386208/1‑150 (MQ=255)
                                                                                            tGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCATTACATGATTATGAGGCAACGCCATGCATCCACGTTTTCAAACCGCTTTTGcc                                                          <  1:4232696/150‑1 (MQ=255)
                                                                                            tGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCATTACATGATTATGAGGCAACGCCATGCATCCACGTTTTCAAACCGCTTTTGcc                                                          <  1:4231547/150‑1 (MQ=255)
                                                                                            tGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCATTACATGATTATGAGGCAACGCCATGCATCCACGTTTTCAAACCGCTTTTGcc                                                          >  1:1924023/1‑150 (MQ=255)
                                                                                            tGCGTCGCGCATTTTTGATGTAAGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCATTACATGATTATGAGGCAACGCCATGCATCCACGTTTTCAAACCGCttattcc                                                          >  2:4336222/1‑145 (MQ=255)
                                                                                                tCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATTACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCATTACATGATTATGAGGCAACGCCATGCATCCACGTTTTCAAACCGCTTTTGCCCAAc                                                      <  1:1475835/150‑1 (MQ=255)
                                                                                                    gcATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGAGTCAGATCACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCATTACATGATTATGAGGCAACGCCATGCATCCACGTTTTCAAACCGCTTTTGCCCAACTTGc                                                  <  1:4295478/150‑1 (MQ=255)
                                                                                                     cATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCATTACATGATTATGAGGCAACGCCATGCATCCACGTTTTCAAACCGCTTTTGCCCAACTTGCg                                                 >  1:1130300/1‑150 (MQ=255)
                                                                                                         tttGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCATTACATGATTATGAGGCAACGCCATGCATCCACGTTTTCAAACCGCTTTTGCCCAACTTGCGGATa                                             >  2:3804754/1‑150 (MQ=255)
                                                                                                         tttGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCATTACATGATTATGAGGCAACGCCATGCATCCACGTTTTCAAACCGCTTTTGCCCAACTTGCGGATa                                             >  2:3804683/1‑150 (MQ=255)
                                                                                                            gATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCATTACATGATTATGAGGCAACGCCATGCATCCACGTTTTCAAACCGCTTTTGCCCAACTTGCGGATAACt                                          <  1:491270/150‑1 (MQ=255)
                                                                                                                tatgtTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCATTACATGATTATGAGGCAACGCCATGCATCCACGTTTTCAAACCGCTTTTGCCCAACTTGCGGATAACTTGCa                                      >  1:4051224/1‑150 (MQ=255)
                                                                                                                  tgtTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCATTACATGATTATGAGGCAACGCCATGCATCCACGTTTTCAAACCGCTTTTGCCCAACTTGCGGATAACTTGCAAt                                    <  1:1773904/150‑1 (MQ=255)
                                                                                                                    tttCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCATTACATGATTATGAGGCAACGCCATGCATCCACGTTTTCAAACCGCTTTTGCCCAACTTGCGGATAACTTGCAATCt                                  <  2:1916113/150‑1 (MQ=255)
                                                                                                                      tCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCATTACATGATTATGAGGCAACGCCATGCATCCACGTTTTCAAACCGCTTTTGCCCAACTTGCGGATAACTTGCAATCTGc                                >  1:2019885/1‑150 (MQ=255)
                                                                                                                          gcgTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCATTACATGATTATGAGGCAACGCCATGCATCCACGTTTTCAAACCGCTTTTGCCCAACTTGCGGATAACTTGCAATCTGCACTg                            <  1:1190151/150‑1 (MQ=255)
                                                                                                                            gTTGCATAATTAATGAGATTCAGATTACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCATTACATGATTATGAGGCAACGCCATGCATCCACGTTTTCAAACCGCTTTTGCCCAACTTGCGGATAACTTGCAATCTGCACTGGa                          >  1:462633/1‑150 (MQ=255)
                                                                                                                             ttGCATAATTAATGAGATTCAGATTACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCATTACATGATTATGAGGCAACGCCATGCATCCACGTTTTCAAACCGCTTTTGCCCAACTTGCGGATAACTTGCAATCTGCACTGGaa                         <  1:998767/150‑1 (MQ=255)
                                                                                                                             ttGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCATTACATGATTATGAGGCAACGCCATGCATCCACGTTTTCAAACCGCTTTTGCCCAACTTGCGGATAACTTGCAATCTGCACTGGaa                         >  2:1702054/1‑150 (MQ=255)
                                                                                                                              tGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCATTACATGATTATGAGGCAACGCCATGCATCCACGTTTTCAAACCGCTTTTGCCCAACTTGCGGATAACTTGCAATCTGCACTGGAAc                        >  1:1274472/1‑150 (MQ=255)
                                                                                                                               gCATAATTAATGAAATTCAGATCACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCATTACATGATTATGAGGCAACGCCATGCATCCACGTTTTCAAACCGCTTTTGCCCAACTTGCGGATAACTTGCAATCTGCACTGGAAcc                       <  1:2236552/150‑1 (MQ=255)
                                                                                                                                cATAATTAATGAGATTCAGATTACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCATTACATGATTATGAGGCAACGCCATGCATCCACGTTTTCAAACCGCTTTTGCCCAACTTGCGGATAACTTGCAATCTGCACTGGAACCt                      >  1:3292677/1‑150 (MQ=255)
                                                                                                                                cATAATTAATGAGATTCAGATTACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCATTACATGATTATGAGGCAACGCCATGCATCCACGTTTTCAAACCGCTTTTGCCCAACTTGCGGATAACTTGCAATCTGCACTGGAACCt                      >  1:2273735/1‑150 (MQ=255)
                                                                                                                                cATAATTAATGAGATTCAGATCACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCATTACATGATTATGAGGCAACGCCATGCATCCACGTTTTCAAACCGCTTTTGCCCAACTTGCGGATAACTTGCAATCTGCACTGGAACCt                      >  1:1661551/1‑150 (MQ=255)
                                                                                                                                  taattaatGAGATTCAGATCACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCATTACATGATTATGAGGCAACGCCATGCATCCACGTTTTCAAACCGCTTTTGCCCAACTTGCGGATAACTTGCAATCTGCACTGGAACCTAt                    <  1:1218569/150‑1 (MQ=255)
                                                                                                                                    attaatGAGATTCAGATCACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCATTACATGATTATGAGGCAACGCCATGCATCCACGTTTTCAAACCGCTTTTGCCCAACTTGCGGATAACTTGCAATCTGCACTGGAACCTATTc                  >  2:2017889/1‑150 (MQ=255)
                                                                                                                                      taatGAGATTCAGATCACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCATTACATGATTATGAGGCAACGCCATGCATCCACGTTTTCAAACCGCTTTTGCCCAACTTGCGGATAACTTGCAATCTGCACTGGAACCTATTCTg                <  1:1021991/150‑1 (MQ=255)
                                                                                                                                      taatGAGATTCAGATCACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCATTACATGATTATGAGGCAACGCCATGCATCCACGTTTTCAAACCGCTTTTGCCCAACTTGCGGATAACTTGCAATCTGCACTGGAACCTATTCTg                <  1:3397621/150‑1 (MQ=255)
                                                                                                                                        atGAGATTCAGATTACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCATTACATGATTATGAGGCAACGCCATGCATCCACGTTTTCAAACCGCTTTTGCCCAACTTGCGGATAACTTGCAATCTGCACTGGAACCTATTCTGGc              >  2:292595/1‑150 (MQ=255)
                                                                                                                                        atGAGATTCAGATCACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCATTACATGATTATGAGGCAACGCCATGCATCCACGTTTTCAAACCGCTTTTGCCCAACTTGCGGATAACTTGCAATCTGCACTGGAACCTATTCTGGc              >  2:3220711/1‑150 (MQ=255)
                                                                                                                                          gagaTTCAGATCACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCATTACATGATTATGAGGCAACGCCATGCATCCACGTTTTCAAACCGCTTTTGCCCAACTTGCGGATAACTTGCAATCTGCACTGGAACCTATTCTGGCAg            <  1:2580368/150‑1 (MQ=255)
                                                                                                                                          gagaTTCAGATCACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCATTACATGATTATGAGGCAACGCCATGCATCCACGTTTTCAAACCGCTTTTGCCCAACTTGCGGATAACTTGCAATCTGCACTGGAACCTATTCTGGCAg            <  1:1838893/150‑1 (MQ=255)
                                                                                                                                            gaTTCAGATCACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCATTACATGATTATGAGGCAACGCCATGCATCCACGTTTTCAAACCGCTTTTGCCCAACTTGCGGATAACTTGCAATCTGCACTGGAACCTATTCTGGCAGAc          <  1:3915029/150‑1 (MQ=255)
                                                                                                                                             aTTCAGATTACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCATTACATGATTATGAGGCAACGCCATGCATCCACGTTTTCAAACCGCTTTTGCCCAACTTGCGGATAACTTGCAATCTGCACTGGAACCTATTCTGGCAGACa         >  1:390274/1‑150 (MQ=255)
                                                                                                                                             aTTCAGATCACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCATTACATGATTATGAGGCAACGCCATGCATCCACGTTTTCAAACCGCTTTTGCCCAACTTGCGGATAACTTGCAATCTGCACTGGAACCTATTCTGGCAGACa         >  2:2877679/1‑150 (MQ=255)
                                                                                                                                             aTTCAGATCACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCATTACATGATTATGAGGCAACGCCATGCATCCACGTTTTCAAACCGCTTTTGCCCAACTTGCGGATAACTTGCAATCTGCACTGGAACCTATTCTGGCAGACa         >  2:2457267/1‑150 (MQ=255)
                                                                                                                                             aTTCAGATCACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCATTACATGATTATGAGGCAACGCCATGCATCCACGTTTTCAAACCGCTTTTGCCCAACTTGCGGATAACTTGCAATCTGCACTGGAACCTATTCTGGCAGACa         >  1:1116524/1‑150 (MQ=255)
                                                                                                                                              ttCAGATTACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCATTACATGATTATGAGGCAACGCCATGCATCCACGTTTTCAAACCGCTTTTGCCCAACTTGCGGATAACTTGCAATCTGCACTGGAACCTATTCTGGCAGACaa        >  1:4064908/1‑150 (MQ=255)
                                                                                                                                              ttCAGATCACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCATTACATGATTATGAGGCAACGCCATGCATCCACGTTTTCAAACCGCTTTTGCCCAACTTGCGGATAACTTGCAATCTGCACTGGAACCTATTCTGGCAGACaa        <  2:4209646/150‑1 (MQ=255)
                                                                                                                                                cAGATCACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCATTACATGATTATGAGGCAACGCCATGCATCCACGTTTTCAAACCGCTTTTGCCCAACTTGCGGATAACTTGCAATCTGCACTGGAACCTATTCTGGCAGACAAGt      <  2:3989480/150‑1 (MQ=255)
                                                                                                                                                 aGATCACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCATTACATGATTATGAGGCAACGCCATGCATCCACGTTTTCAAACCGCTTTTGCCCAACTTGCGGATAACTTGCAATCTGCACTGGAACCTATTCTGGCAGACAAGTa     >  2:3723202/1‑150 (MQ=255)
                                                                                                                                                 aGATCACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCATTACATGATTATGAGGCAACGCCATGCATCCACGTTTTCAAACCGCTTTTGCCCAACTTGCGGATAACTTGCAATCTGCACTGGAACCTATTCTGGCAGACAAGTa     <  1:840798/150‑1 (MQ=255)
                                                                                                                                                 aGATCACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCATTACATGATTATGAGGCAACGCCATGCATCCACGTTTTCAAACCGCTTTTGCCCAACTTGCGGATAACTTGCAATCTGCACTGGAACCTATTCTGGCAGACAAGTa     >  2:18506/1‑150 (MQ=255)
                                                                                                                                                   aTCACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCATTACATGATTATGAGGCAACGCCATGCATCCACGTTTTCAAACCGCTTTTGCCCAACTTGCGGATAACTTGCAATCTGCACTGGAACCTATTCTGGCAGACAAGTACt   >  2:871143/1‑150 (MQ=255)
                                                                                                                                                    tCACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCATTACATGATTATGAGGCAACGCCATGCATCCACGTTTTCAAACCGCTTTTGCCCAACTTGCGGATAACTTGCAATCTGCACTGGAACCTATTCTGGCAGACAAGTACtt  <  1:2231078/150‑1 (MQ=255)
                                                                                                                                                    tCACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCATTACATGATTATGAGGCAACGCCATGCATCCACGTTTTCAAACCGCTTTTGCCCAACTTGCGGATAACTTGCAATCTGCACTGGAACCTATTCTGGCAGACAAGTACtt  <  1:1521157/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
CGCTAGCGCTGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGCTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCATTACATGATTATGAGGCAACGCCATGCATCCACGTTTTCAAACCGCTTTTGCCCAACTTGCGGATAACTTGCAATCTGCACTGGAACCTATTCTGGCAGACAAGTACTT  >  CP009273/2225111‑2225408

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A