Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F142 I0 R1 859 274.6 8723680 96.4% 8409627 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,058,692 C→T 14.7% P178S (CCT→TCT)  scpC → propionyl‑CoA:succinate CoA transferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,058,6920CT14.7% 853.6 / 113.1 292P178S (CCT→TCT) scpCpropionyl‑CoA:succinate CoA transferase
Reads supporting (aligned to +/- strand):  ref base C (121/128);  new base T (23/20);  total (144/148)
Fisher's exact test for biased strand distribution p-value = 6.21e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 295 total aligned reads displayed.

AAGCATCGGCACTGGCACCGGATGGTCGAGTCTGGTTAACCAGCGGGATCGGTAATGCGCCGACCTGGCTGCTGCGGGCGAAGAAAGTGATCATTGAACTCAATCACTATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATCGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGGATAAGCAAAAT  >  CP009273/3058543‑3058841
                                                                                                                                                     |                                                                                                                                                     
aaGCATCGGCACTGGCACCGGATGGTCGAGTCTGGTTAACCAGCGGGATCGGTAATGCGCCGACCTGGCTGCTGCGGGCGAAGAAAGTGATCATTGAACTCAATCACTATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTc                                                                                                                                                       <  2:1446411/150‑1 (MQ=255)
  gCATCGGCACTGGCACCGGATGGTCGAGTCTGGTTAACCAGCGGGATCGGTAATGCGCCGACCTGGCTGCTGCGGGCGAAGAAAGTGATCATTGAACTCAATCACTATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCt                                                                                                                                                     <  1:730894/150‑1 (MQ=255)
  gCATCGGCACTGGCACCGGATGGTCGAGTCTGGTTAACCAGCGGGATCGGTAATGCGCCGACCTGGCTGCTGCGGGCGAAGAAAGTGATCATTGAACTCAATCACTATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCt                                                                                                                                                     <  1:1253337/150‑1 (MQ=255)
   cATCGGCACTGGCACCGGATGGTCGAGTCTGGTTAACCAGCGGGATCGGTAATGCGCCGACCTGGCTGCTGCGGGCGAAGAAAGTGATCATTGAACTCAATCACTATCACGATCCGCGCGTTGCAGAACTGGCGGATCTTGTGATTCCTg                                                                                                                                                    >  2:2479337/1‑150 (MQ=255)
   cATCGGCACTGGCACCGGATGGTCGAGTCTGGTTAACCAGCGGGATCGGTAATGCGCCGACCTGGCTGCTGCGGGCGAAGAAAGTGATCATTGAACTCAATCACTATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTg                                                                                                                                                    >  2:1713322/1‑150 (MQ=255)
   cATCGGCACTGGCACCGGATGGTCGAGTCTGGTTAACCAGCGGGATCGGTAATGCGCCGACCTGGCTGCTGCGGGCGAAGAAAGTGATCATTGAACTCAATCACTATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTg                                                                                                                                                    <  2:3844034/150‑1 (MQ=255)
   cATCGGCACTGGCACCGGATGGTCGAGTCTGGTTAACCAGCGGGATCGGTAATGCGCCGACCTGGCTGCTGCGGGCGAAGAAAGTGATCATTGAACTCAATCACTATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTg                                                                                                                                                    <  2:3341312/150‑1 (MQ=255)
      cGGCACTGGCACCGGATGGTCGAGTCTGGTTAACCAGCGGGATCGGTAATGCGCCGACCTGGCTGCTGCGGGCGAAGAAAGTGATCATTGAACTCAATCACTATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGgcg                                                                                                                                                 >  2:1790042/1‑150 (MQ=255)
        gCACTGGCACCGGATGGTCGAGTCTGGTTAACCAGCGGGATCGGTAATGCGCCGACCTGGCTGCTGCGGGCGAAGAAAGTGATCATTGAACTCAATCACTATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTTCTGgcgcg                                                                                                                                               <  1:100486/150‑1 (MQ=255)
        gCACTGGCACCGGATGGTCGAGTCTGGTTAACCAGCGGGATCGGTAATGCGCCGACCTGGCTGCTGCGGGCGAAGAAAGTGATCATTGAACTCAATCACTATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTTCTGgcgcg                                                                                                                                               <  1:3794576/150‑1 (MQ=255)
        gCACTGGCACCGGATGGTCGAGTCTGGTTAACCAGCGGGATCGGTAATGCGCCGACCTGGCTGCTGCGGGCGAAGAAAGTGATCATTGAACTCAATCACTATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGgcgcg                                                                                                                                               <  1:2934064/150‑1 (MQ=255)
          aCTGGCACCGGATGGTCGAGTCTGGTTAACCAGCGGGATCGGTAATGCGCCGACCTGGCTGCTGCGGGCGAAGAAAGTGATCATTGAACTCAATCACTATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGcc                                                                                                                                             <  1:1731643/150‑1 (MQ=255)
                aCCGGATGGTCGAGTCTGGTTAACCAGCGGGATCGGTAATGCGCCGACCTGGCTGCTGCGGGCGAAGAAAGTGATCATTGAACTCAATCACTATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACcgcg                                                                                                                                       <  2:652795/150‑1 (MQ=255)
                  cGGATGGTCGAGTCTGGTTAACCAGCGGGATCGGTAATGCGCCGACCTGGCTGCTGCGGGCGAAGAAAGTGATCATTGAACTCAATCACTATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCgcggc                                                                                                                                     <  1:2931138/150‑1 (MQ=255)
                       ggTCGAGTCTGGTTAACCAGCGGGATCGGTAATGCGCCGACCTGGCTGCTGCGGGCGAAGAAAGTGATCATTGAACTCAATCACTATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAAt                                                                                                                                <  2:3966345/150‑1 (MQ=255)
                       ggTCGAGTCTGGTTAACCAGCGGGATCGGTAATGCGCCGACCTGGCTGCTGCGGGCGAAGAAAGTGATCATTGAACTCAATCACTATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAAt                                                                                                                                <  2:2153904/150‑1 (MQ=255)
                         tCGAGTCTGGTTAACCAGCGGGATCGGTAATGCGCCGACCTGGCTGCTGCGGGCGAAGAAAGTGATCATTGAACTCAATCACTATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAg                                                                                                                              >  2:3746206/1‑150 (MQ=255)
                             gTCTGGTTAACCAGCGGGATCGGTAATGCGCCGACCTGGCTGCTGCGGGCGAAGAAAGTGATCATTGAACTCAATCACTATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTTCTGGCGCGCCACCGCGGCGCAATAGCgtg                                                                                                                          <  2:3002950/150‑1 (MQ=255)
                               cTGGTTAACCAGCGGGATCGGTAATGCGCCGACCTGGCTGCTGCGGGCGAAGAAAGTGATCATTGAACTCAATCACTATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTc                                                                                                                        <  1:1442848/150‑1 (MQ=255)
                                 ggTTAACCAGCGGGATCGGTAATGCGCCGACCTGGCTGCTGCGGGCGAAGAAAGTGATCATTGAACTCAATCACTATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGa                                                                                                                      <  1:760250/150‑1 (MQ=255)
                                     aaCCAGCGGGATCGGTAATGCGCCGACCTGGCTGCTGCGGGCGAAGAAAGTGATCATTGAACTCAATCACTATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCtt                                                                                                                  >  2:2054375/1‑150 (MQ=255)
                                       ccAGCGGGATCGGTAATGCGCCGACCTGGCTGCTGCGGGCGAAGAAAGTGATCATTGAACTCAATCACTATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTcc                                                                                                                >  2:1262815/1‑150 (MQ=255)
                                          gCGGGATCGGTAATGCGCCGACCTGGCTGCTGCGGGCGAAGAAAGTGATCATTGAACTCAATCACTATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATg                                                                                                             <  1:621643/150‑1 (MQ=255)
                                             ggATCGGTAATGCGCCGACCTGGCTGCTGCGGGCGAAGAAAGTGATCATTGAACTCAATCACTATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCaa                                                                                                          <  2:4162851/150‑1 (MQ=255)
                                              gATCGGTAATGCGCCGACCTGGCTGCTGCGGGCGAAGAAAGTGATCATTGAACTCAATCACTATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAAt                                                                                                         >  2:3616490/1‑150 (MQ=255)
                                               aTCGGTAATGCGCCGACCTGGCTGCTGCGGGCGAAGAAAGTGATCATTGAACTCAATCACTATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTTCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATg                                                                                                        >  1:281178/1‑150 (MQ=255)
                                                  ggTAATGCGCCGACCTGGCTGCTGCGGGCGAAGAAAGTGATCATTGAACTCAATCACTATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTTCTGGCGCGCCCCCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGAt                                                                                                     >  1:146769/1‑150 (MQ=255)
                                                  ggTAATGCGCCGACCTGGCTGCTGCGGGCGAAGAAAGTGATCATTGAACTCAATCACTATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGAt                                                                                                     <  2:2631655/150‑1 (MQ=255)
                                                   gTAATGCGCCGACCTGGCTGCTGCGGGCGAAGACAGTGATCATTGAACTCACTCACTATCACGCTCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATc                                                                                                    <  2:3244519/150‑1 (MQ=255)
                                                   gTAATGCGCCGACCTGGCTGCTGCGGGCGAAGAAAGTGATCATTGAACTCAATCACTATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATc                                                                                                    <  1:103885/150‑1 (MQ=255)
                                                     aaTGCGCCGACCTGGCTGCTGCGGGCGAAGAAAGTGATCATTGAACTCAATCACTATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTTCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATcgc                                                                                                  >  2:2392379/1‑150 (MQ=255)
                                                      aTGCGCCGACCTGGCTGCTGCGGGCGAAGAAAGTGATCATTGAACTCAATCACTATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATcgcg                                                                                                 >  2:1843697/1‑150 (MQ=255)
                                                         cgcCGACCTGGCTGCTGCGGGCGAAGAAAGTGATCATTGAACTCAATCACTATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCg                                                                                              <  2:83229/150‑1 (MQ=255)
                                                           ccGACCTGGCTGCTGCGGGCGAAGAAAGTGATCATTGAACTCAATCACTATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGt                                                                                            >  1:3119391/1‑150 (MQ=255)
                                                           ccGACCTGGCTGCTGCGGGCGAAGAAAGTGATCATTGAACTCAATCACTATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGt                                                                                            >  1:4229698/1‑150 (MQ=255)
                                                              aCCTGGCTGCTGCGGGCGAAGAAAGTGATCATTGAACTCAATCACTATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTAcc                                                                                         <  2:1993404/150‑1 (MQ=255)
                                                              aCCTGGCTGCTGCGGGCGAAGAAAGTGATCATTGAACTCAATCACTATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTAcc                                                                                         >  1:753030/1‑150 (MQ=255)
                                                              aCCTGGCTGCTGCGGGCGAAGAAAGTGATCATTGAACTCAATCACTATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTAcc                                                                                         <  1:958501/150‑1 (MQ=255)
                                                                 tGGCTGCTGCGGGCGAAGAAAGTGATCATTGAACTCAATCACTATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTTCTGGCGCGCCCCCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGc                                                                                      >  2:2594599/1‑150 (MQ=255)
                                                                  ggCTGCTGCGGGCGAAGAAAGTGATCATTGAACTCAATCACTATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCt                                                                                     >  2:1702566/1‑150 (MQ=255)
                                                                   gctgctGCGGGCGAAGAAAGTGATCATTGAACTCAATCACTATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTTCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTa                                                                                    >  2:1334810/1‑150 (MQ=255)
                                                                     tgctgcGGGCGAAGAAAGTGATCATTGAACTCAATCACTATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTAtg                                                                                  <  1:1107695/150‑1 (MQ=255)
                                                                     tgctgcGGGCGAAGAAAGTGATCATTGAACTCAATCACTATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTAtg                                                                                  <  1:619298/150‑1 (MQ=255)
                                                                      gctgcGGGCGAAGAAAGTGATCATTGAACTCAATCACTATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTAtgt                                                                                 <  1:428864/150‑1 (MQ=255)
                                                                        tgcGGGCGAAGAAAGTGATCATTGAACTCAATCACTATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGc                                                                               >  2:122244/1‑150 (MQ=255)
                                                                            ggCGAAGAAAGTGATCATTGAACTCAATCACTATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAAt                                                                           <  1:3051301/150‑1 (MQ=255)
                                                                            ggCGAAGAAAGTGATCATTGAACACAATCACTATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAAt                                                                           >  1:289491/1‑150 (MQ=255)
                                                                              cGAAGAAAGTGATCATTGAACTCAATCACTATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTTCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATCg                                                                         <  2:1588811/150‑1 (MQ=255)
                                                                              cGAAGAAAGTGATCATTGAACTCAATCACTATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAAttg                                                                         <  1:3478083/150‑3 (MQ=255)
                                                                               gaagaaAGTGATCATTGAACTCAATCACTATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGa                                                                        <  2:892797/150‑1 (MQ=255)
                                                                               gaagaaAGTGATCATTGAACTCAATCACTATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGa                                                                        >  1:3985063/1‑150 (MQ=255)
                                                                                aagaaAGTGATCATTGAACTCAATCACTATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGAt                                                                       >  1:366881/1‑150 (MQ=255)
                                                                                aagaaAGTGATCATTGAACTCAATCACTATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGAt                                                                       <  1:451082/150‑1 (MQ=255)
                                                                                 agaaAGTGATCATTGAACTCAATCACTATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATCGATc                                                                      >  2:1187167/1‑150 (MQ=255)
                                                                                    aaGTGATCATTGAACTCAATCACTATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGa                                                                   >  2:3673871/1‑150 (MQ=255)
                                                                                    aaGTGATCATTGAACTCAATCACTATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCAAGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGa                                                                   >  2:2164075/1‑150 (MQ=255)
                                                                                     aGTGATCATTGAACTCAATCACTATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGaa                                                                  >  2:2039213/1‑150 (MQ=255)
                                                                                     aGTGATCATTGAACTCAATCACTATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATCGATCCGaa                                                                  >  2:2203559/1‑150 (MQ=255)
                                                                                       tGATCATTGAACTCAATCACTATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGaaaa                                                                >  1:416289/1‑150 (MQ=255)
                                                                                        gATCATTGAACTCAATCACTATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGaaaaa                                                               >  1:2427418/1‑150 (MQ=255)
                                                                                         aTCATTGAACTCAATCACTATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATCGATCCGAAAAAg                                                              >  1:2065179/1‑150 (MQ=255)
                                                                                           cATTGAACTCAATCACTATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGAt                                                            >  1:4170954/1‑150 (MQ=255)
                                                                                           cATTGAACTCAATCACTATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGAt                                                            >  1:1983900/1‑150 (MQ=255)
                                                                                              tGAACTCAATCACTATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGt                                                         >  1:518186/1‑150 (MQ=255)
                                                                                                aaCTCAATCACTATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATCGATCCGAAAAAGATTGTCg                                                       >  1:2625170/1‑150 (MQ=255)
                                                                                                    cAATCACTATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCcgt                                                   >  1:1417395/1‑150 (MQ=255)
                                                                                                      aTCACTATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCcgtcg                                                 >  2:1168678/1‑150 (MQ=255)
                                                                                                      aTCACTATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCcgtcg                                                 <  2:1744026/150‑1 (MQ=255)
                                                                                                       tCACTATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAGAAAGATTGTCGCcgtcgt                                                >  2:4237228/1‑150 (MQ=255)
                                                                                                        cACTATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTTCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATCGATCCGAAAAAGATTGTCGCCGTCGTg                                               >  1:603271/1‑150 (MQ=255)
                                                                                                        cACTATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATCGATCCGAAAAAGATTGTCGCCGTCGTg                                               <  2:3895305/150‑1 (MQ=255)
                                                                                                              cACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTTCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATCGATCCGAAAAAGATTGTCGCCGTCGTGGAAAcc                                         >  1:1326894/1‑150 (MQ=255)
                                                                                                              cACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTTCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATCGATCCGAAAAAGATTGTCGCCGTCGTGGAAAcc                                         <  1:2644581/150‑1 (MQ=255)
                                                                                                                  aTCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACt                                     <  1:4319821/150‑1 (MQ=255)
                                                                                                                  aTCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACt                                     >  1:3252357/1‑150 (MQ=255)
                                                                                                                   tCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACtt                                    >  1:3932853/1‑150 (MQ=255)
                                                                                                                   tCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACtt                                    <  1:1683428/150‑1 (MQ=255)
                                                                                                                    ccGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATCGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTg                                   <  1:1829175/150‑1 (MQ=255)
                                                                                                                     cgcgcgTTGCAGAACTGGCGGATATTGTGATTTCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATCGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGc                                  <  2:672986/150‑1 (MQ=255)
                                                                                                                      gcgcgTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATCGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGcc                                 <  1:3270634/150‑1 (MQ=255)
                                                                                                                       cgcgTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATCGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGccc                                >  2:267092/1‑150 (MQ=255)
                                                                                                                         cgTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATCGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGa                              >  1:407241/1‑150 (MQ=255)
                                                                                                                          gTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGAc                             >  2:4315835/1‑150 (MQ=255)
                                                                                                                             gCAGAACTGGCGGATATTGTGATTTCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATCGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGcc                          <  2:286076/150‑1 (MQ=255)
                                                                                                                               aGAACTGGCGGATATTGTGATTTCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATCGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCgg                        >  1:124445/1‑150 (MQ=255)
                                                                                                                               aGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCgg                        >  2:731844/1‑150 (MQ=255)
                                                                                                                               aGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCgg                        >  1:3386047/1‑150 (MQ=255)
                                                                                                                                 aaCTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATCGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTa                      <  1:4328753/150‑1 (MQ=255)
                                                                                                                                  aCTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATCGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTaa                     <  1:1561379/150‑1 (MQ=255)
                                                                                                                                    tGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAata                   <  2:3450969/150‑1 (MQ=255)
                                                                                                                                    tGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAata                   >  2:1981218/1‑150 (MQ=255)
                                                                                                                                      gCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATCGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATg                 <  2:594802/150‑1 (MQ=255)
                                                                                                                                        ggATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCt               >  2:4045866/1‑150 (MQ=255)
                                                                                                                                           tatTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATCGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGGa            >  1:3009007/1‑150 (MQ=255)
                                                                                                                                            atTGTGATTTCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATCGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGGAt           <  2:3017884/150‑1 (MQ=255)
                                                                                                                                            atTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATCGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGGAt           <  1:1380531/150‑1 (MQ=255)
                                                                                                                                             ttGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATCGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGGATa          >  2:3140415/1‑150 (MQ=255)
                                                                                                                                               gtgATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATCGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGGATAAg        <  2:1126359/150‑1 (MQ=255)
                                                                                                                                                 gATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGGATAAGCa      >  2:3537660/1‑150 (MQ=255)
                                                                                                                                                  aTTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGGATAAGCaa     <  1:3600726/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
AAGCATCGGCACTGGCACCGGATGGTCGAGTCTGGTTAACCAGCGGGATCGGTAATGCGCCGACCTGGCTGCTGCGGGCGAAGAAAGTGATCATTGAACTCAATCACTATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATCGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGGATAAGCAAAAT  >  CP009273/3058543‑3058841

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A