Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F534 I1 R1	1235	230.2	7611948	95.1%	7238962	150.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP009273	375,478	(G)_10→11	intergenic (‑141/+47)	frmR ← / ← yaiO	regulator protein that represses frmRAB operon/outer membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	375,468	1	.	G	84.5%	317.8 / 56.3	97	intergenic (‑131/+57)	frmR/yaiO	regulator protein that represses frmRAB operon/outer membrane protein
Reads supporting (aligned to +/- strand): ref base . (12/3); new base G (79/3); total (91/6)
Fisher's exact test for biased strand distribution p-value = 4.56e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 114 total aligned reads displayed.

CTTCCGGAGTACTGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑‑GG‑GGGGGGGG‑CTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGTTGGGGTGTCGTACCACACTTTACCTGCCGTCAGCCCGAG  >  CP009273/375319‑375617

cttcCGGAGTACTGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGaaa                                                                                                                                                           <  2:3489314/150‑1 (MQ=255)

 ttcCGGAGTACTGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑‑g                                                                                                                                                        <  1:3744309/150‑1 (MQ=255)

 ttcCGGAGTACTGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑‑g                                                                                                                                                        >  1:2631708/1‑150 (MQ=255)

 ttcCGGAGTACTGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑‑g                                                                                                                                                        <  1:1483265/150‑1 (MQ=255)

 ttcCGGAGAACTGGGCATTTCGCACCTCATCATCAGCATGCAATAAACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATATATCCGGTGACTGTGGTCGGAAAACAAAGAGGATA‑‑g                                                                                                                                                        >  2:3332939/1‑150 (MQ=255)

  tcCGGAGTACTGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑‑gg                                                                                                                                                       <  2:2366891/150‑1 (MQ=255)

  tcCGGAGTACTGGGCATTTCGCACCTCATCATCTGCATGCAAGATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑‑gg                                                                                                                                                       <  2:2366910/150‑1 (MQ=255)

    cGGAGTACTGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑‑gg‑gg                                                                                                                                                    <  1:1985775/150‑1 (MQ=255)

    cGGAGTACTGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑‑gg‑gg                                                                                                                                                    <  1:2834471/150‑1 (MQ=255)

    cGGAGTACTGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑‑gg‑gg                                                                                                                                                    <  1:929219/150‑1 (MQ=255)

    cGGAGTACTGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑‑gg‑gg                                                                                                                                                    <  2:1195677/150‑1 (MQ=255)

       aGTACTGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAC‑‑gg‑ggggg                                                                                                                                                 >  2:3044346/1‑150 (MQ=255)

       aGTACTGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑‑gg‑ggggg                                                                                                                                                 <  1:828314/150‑1 (MQ=255)

            tGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGACA‑‑gg‑gggggggggc                                                                                                                                            >  2:1898806/1‑148 (MQ=255)

                 aTTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAG‑GG‑GGGGGGGG‑CTAATc                                                                                                                                       >  2:3556721/1‑150 (MQ=255)

                     cGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAG‑GG‑GGGGGGGG‑CTAATCGGCa                                                                                                                                   >  2:196033/1‑150 (MQ=255)

                      gCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAAGGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGACTGTGGTCGGAAAACAACGAGGAAA‑‑GG‑GGGGGGGG‑CTAATCGGCAgg                                                                                                                                 <  2:2977705/150‑1 (MQ=255)

                       caccacATCATCTGCATGCAATATACTACCGGGGGGTATTCTAAATGACAATGCATACCCCCCTATAGTATATAACACGGTTGAATAAAATTTATCCGGTGAATGTGGTCTGAAAACAAAGAGGAAAG‑GG‑GGGGGGGG‑CTAATCGGCAgg                                                                                                                                 <  2:194601/150‑1 (MQ=255)

                        aCCTCATCATCTGCATGCAATATACTATTTTGGGGTATTCTATATGTCAATGCATACACACCTATTTTATATAACACTGGAGAATAAAATTTATCCTGTGAATGTGGTCTTAAACAAACGAGGAAA‑‑GG‑TGGTGGGT‑CTAATCGGCAGGGa                                                                                                                               <  2:3542428/150‑1 (MQ=255)

                         ccTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑‑GG‑GGGGGGGG‑CTAATCGGCAGGGaa                                                                                                                              >  1:407003/1‑150 (MQ=255)

                          cTCATCATCAGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACAGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAG‑GG‑GGGGGGGG‑CTAATCGGCAGGGaa                                                                                                                              >  2:910454/1‑150 (MQ=255)

                           taataaccTGCTTGAAATATACTATCGGGGGTTATTCAATATGCCAAGGCAGACCCCCCTTTAGTATAAACCCCTGGAGAATAAAATTTAGCCGGTGAAGGGGGCCGCAAAACAAACAGCAAAG‑GG‑GGGGGGGG‑CTAATCGGCAGGGAAg                                                                                                                             <  2:1330526/143‑1 (MQ=255)

                               catcTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAATGAGGAAAG‑GG‑GGGGGGGG‑CTAATCGGCAGGGAAGgccg                                                                                                                         >  2:459488/1‑150 (MQ=255)

                               catcTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAG‑GG‑GGGGGGGG‑CTAATCGGCAGGGAAGgccg                                                                                                                         >  2:395799/1‑150 (MQ=255)

                               catcTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAG‑GG‑GGGGGGGG‑CTAATCGGCAGGGAAGgccg                                                                                                                         >  2:901038/1‑150 (MQ=255)

                               catcTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAG‑GG‑GGGGGGGG‑CTAATAGGGAGGGAAGgccg                                                                                                                         >  2:395589/1‑150 (MQ=255)

                               catcTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑‑GG‑GGGGGGGG‑CTAATCGGCAGGGAAGgccgc                                                                                                                        >  1:1224073/1‑150 (MQ=255)

                                   tgcatgcaATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAG‑GG‑GGGGGGGG‑CTAATCGGCAGGGAAGGCCGcccc                                                                                                                     >  1:1017374/1‑150 (MQ=255)

                                   tgcatgcaATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAG‑GG‑GGGGGGGG‑CTAATAGGCAGGGAAGGCGGcccc                                                                                                                     >  1:1017363/1‑150 (MQ=255)

                                    gcatgcaATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAG‑GG‑GGGGGGGG‑CTAATCGGCAGGGAAGGCCGCCCCg                                                                                                                    >  1:2509700/1‑150 (MQ=255)

                                      atgcaATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAG‑GG‑GGGGGGGG‑CTAATCGGCAGGGAAGGCCGCCCCGGa                                                                                                                  >  2:2406849/1‑150 (MQ=255)

                                       tgcaATATACCCTAGGGGTGTATTCTCTATGTCACTGCATACCCCCCTTTAGAATATAACACTGGAGAATAAAATTTCGCCGGTGAATGTGGTCGGAAAACACCGCGGAAAG‑GG‑GGGGGGGG‑CTAATCGGCAGGGAAGGCCGCCCCGGAt                                                                                                                 <  2:3332701/150‑1 (MQ=255)

                                          aaTATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAG‑GG‑GGGGGGGG‑CTAATCAGAAGGGAAGGACGCCCCGGATAgc                                                                                                              >  1:2907783/1‑150 (MQ=255)

                                           atatACTACAGGCGTGTATTCTCTATGACACTGCATACCCCCCTCTAGTATATCACACGGCACAACACCATCTCCCCGGTCACTGGCGCCCCACAACACCCAGCACA‑‑GG‑GGGGGGGG‑CTAATCGGCAGGGAAGGCCGCCCCGGATAgcgg                                                                                                            <  2:1440720/150‑1 (MQ=255)

                                               aCTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑‑GG‑GGGGGGGGACTAATCGGCAGGGAAGGCCGCCCCGGATCgcgggcg                                                                                                         >  2:3406991/1‑150 (MQ=255)

                                                cTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAG‑GG‑GGGGGGGG‑CTAATCGGCAGGGAAGGCCGCCCCGGATAgcgggcgg                                                                                                        >  1:1097859/1‑150 (MQ=255)

                                                cTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAG‑GG‑GGGGGGGG‑CTAATCGGCAGGGAAGGCCGCCCCGGATAgcgggcgg                                                                                                        >  1:3640200/1‑150 (MQ=255)

                                                cTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAG‑GG‑GGGGGGGG‑CTAATCGGCAGGGAAGGCCGCCCCGGATAgcgggcgg                                                                                                        >  1:941268/1‑150 (MQ=255)

                                                 tataGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAG‑GG‑GGGGGGGG‑CTAATCGGCAGGGAAGGCCGCCCCGGATAGCGggcggc                                                                                                       >  1:3373793/1‑150 (MQ=255)

                                                 tataGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAG‑GG‑GGGGGGGG‑CTAATCGGCAGGGAAGGCCGCCCCGGATAGCGggcggc                                                                                                       >  1:3373732/1‑150 (MQ=255)

                                                 tataGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAG‑GG‑GGGGGGGG‑CTAATCGGCAGGGAAGGCCGCCCCGGATAGCGggcggc                                                                                                       >  1:1063261/1‑150 (MQ=255)

                                                  ataGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAG‑GG‑GGGGGGGG‑CTAAACGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCa                                                                                                      >  2:262081/1‑150 (MQ=255)

                                                    aGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑‑GG‑GGGGGGGG‑CTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGaa                                                                                                   >  2:887927/1‑150 (MQ=255)

                                                            aTTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAG‑GG‑GGGGGGGG‑CTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCa                                                                                            >  1:44697/1‑150 (MQ=255)

                                                            aTTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAG‑GG‑GGGGGGGG‑CTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCa                                                                                            >  1:1828648/1‑150 (MQ=255)

                                                              tCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAG‑GG‑GGGGGGGG‑CTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGa                                                                                          >  2:546074/1‑150 (MQ=255)

                                                                   atGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAG‑GG‑GGGGGGGG‑CTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTc                                                                                     >  2:3741368/1‑150 (MQ=255)

                                                                      tCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAG‑GG‑GGGGGGGG‑CTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAg                                                                                  >  1:2477680/1‑150 (MQ=255)

                                                                       cAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAG‑GG‑GGGGGGGG‑CTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGACTCAGAATTTCCAgg                                                                                 >  2:3390209/1‑150 (MQ=255)

                                                                       cAATGCAAACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAG‑GG‑GGGGGGGG‑CTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAgg                                                                                 >  2:849918/1‑150 (MQ=255)

                                                                        aaTGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGG‑GGGGGGGG‑CTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAgg                                                                                 >  1:1299150/1‑150 (MQ=255)

                                                                        aaTGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAG‑GG‑GGGGGGGG‑CTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGt                                                                                >  1:2807842/1‑150 (MQ=255)

                                                                        aaTGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAG‑GG‑GGGGGGGG‑CTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGt                                                                                >  1:2105428/1‑150 (MQ=255)

                                                                        aaTGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAG‑GG‑GGGGGGGG‑CTAATCGGCAGGGAAGGCCGCCCCGGAAAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGt                                                                                >  1:2106898/1‑150 (MQ=255)

                                                                         aTGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑‑GG‑GGGGGGGG‑CTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCa                                                                              >  1:1181176/1‑150 (MQ=255)

                                                                          tGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAG‑GG‑GGGGGGGG‑CTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCa                                                                              >  2:2589277/1‑150 (MQ=255)

                                                                            cATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAG‑GG‑GGGGGGGG‑CTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGa                                                                            >  1:879322/1‑150 (MQ=255)

                                                                            cATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑‑GG‑GGGGGGGG‑CTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGAc                                                                           >  1:3153208/1‑150 (MQ=255)

                                                                               aCCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAG‑GG‑GGGGGGGG‑CTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACgg                                                                         >  2:1906214/1‑150 (MQ=255)

                                                                                cccctcTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑‑GGTGGGGGGGG‑CTAATCGGCGGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACggg                                                                        >  2:1370104/1‑150 (MQ=255)

                                                                                   cccTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAG‑GG‑GGGGGGGG‑CTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTg                                                                     >  1:2355048/1‑150 (MQ=255)

                                                                                   cccTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAG‑GG‑GGGGGGGG‑CTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTg                                                                     >  2:2590798/1‑150 (MQ=255)

                                                                                   cccTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑‑GG‑GGGGGGGG‑CTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGc                                                                    >  1:1716226/1‑150 (MQ=255)

                                                                                    ccTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAG‑GG‑GGGGGGGG‑GCAATAGGGCGGGAACGCCCCCCCGGATAGCCGGGGGCCCAAGGGAGCAGAAATTGCCGGTCACACGggcggc                                                                    >  2:2355678/1‑150 (MQ=255)

                                                                                    ccTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAG‑GG‑GGGGGGGG‑CTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGc                                                                    >  2:450088/1‑150 (MQ=255)

                                                                                    ccTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAG‑GG‑GGGGGGGG‑CTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGc                                                                    >  2:2714174/1‑150 (MQ=255)

                                                                                    ccTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAG‑GG‑GGGGGGGG‑CTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGc                                                                    >  1:950496/1‑150 (MQ=255)

                                                                                    ccTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAG‑GG‑GGGGGGGG‑CTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGc                                                                    >  1:950083/1‑150 (MQ=255)

                                                                                         aGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAG‑GG‑GGGGGGGG‑CTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGtt                                                               >  2:1618144/1‑150 (MQ=255)

                                                                                         aGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAG‑GG‑GGGGGGGG‑CTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGtt                                                               >  1:2916712/1‑150 (MQ=255)

                                                                                           tatataACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAG‑GG‑GGGGGGGG‑CTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGc                                                             >  1:1480024/1‑150 (MQ=255)

                                                                                            atataACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAG‑GG‑GGGGGGGG‑CTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCa                                                            >  2:1870622/1‑150 (MQ=255)

                                                                                             tataACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAG‑GG‑GGGGGGGG‑CTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAg                                                           >  2:165274/1‑150 (MQ=255)

                                                                                              ataACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAG‑GG‑GGGGGGGG‑CTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGa                                                          >  1:1191082/1‑150 (MQ=255)

                                                                                               taACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAG‑GG‑GGGGGGGG‑CTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGAc                                                         >  2:1328812/1‑150 (MQ=255)

                                                                                                aaCACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAG‑GG‑GGGGGGGG‑CTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAAACAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGAcc                                                        >  1:3478501/1‑150 (MQ=255)

                                                                                                aaCACTGGAGAATAAAATTTATCCGGTGAATGTGGCCGGAAAACAAAGAGGAAAG‑GG‑GGGGGGGG‑CTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGAcc                                                        >  1:3121117/1‑150 (MQ=255)

                                                                                                    cTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAG‑GG‑GGGGGGGG‑CTAATAGGGAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTACAGGTAAAAAGGGCTGCAAGTTTCAGACCGGTa                                                    >  2:3668963/1‑150 (MQ=255)

                                                                                                     tGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAG‑GG‑GGGGGGGG‑CTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCACGTTGCAGACCGTTaa                                                   >  2:2697076/1‑150 (MQ=255)

                                                                                                     tGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAG‑GG‑GGGGGGGG‑CTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTaa                                                   >  2:2676136/1‑150 (MQ=255)

                                                                                                           aTAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAG‑GG‑GGGGGGGG‑CTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCATAATTTACAGGTCAAACGGGCTGCAAGTTGCAGAACGTTAAAatcat                                             >  1:1451656/1‑150 (MQ=255)

                                                                                                              aaaTTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAG‑GG‑GGGGGGGG‑CTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCgg                                          >  1:3533190/1‑150 (MQ=255)

                                                                                                              aaaTTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAG‑GG‑GGGGGGGG‑CTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCgg                                          >  1:1187906/1‑150 (MQ=255)

                                                                                                               aaTTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAG‑GG‑GGGGGGGG‑CTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGt                                         >  1:433104/1‑150 (MQ=255)

                                                                                                                 tttATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAG‑GG‑GGGGGGGG‑CTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGTTg                                       >  1:2754650/1‑150 (MQ=255)

                                                                                                                  ttATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAG‑GG‑GGGGGGGG‑CAAAACGCCAGGGAAGGCCCCCCCGAAAAGCGGGGGGCAAAAGAAAACAAAATTACCAGGACAAACGGCCGCCAAGTTCCAACCCGTACAAAACAACGGTTgg                                      >  1:2751432/1‑150 (MQ=255)

                                                                                                                       cGGTGAATGTGGTCGGAAAACAAAGAGGAAAG‑GG‑GGGGGGGG‑CTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGTTGGGgtgt                                 >  2:3789513/1‑150 (MQ=255)

                                                                                                                       cGGTGAATGTGGTCGGAAAACAAAGAGGAAAG‑GG‑GGGGGGGG‑CTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGACAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGTTGGGgtgt                                 >  2:2399969/1‑150 (MQ=255)

                                                                                                                          tGAATGTGGTCGGAAAACAAAGAGGAAAG‑GG‑GGGGGGGG‑CTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGTTGGGGTGTCGt                              >  2:2973876/1‑150 (MQ=255)

                                                                                                                           gAATGTGGTCGGAAAACAAAGAGGAAA‑‑GG‑GGGGGGGG‑CTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGTTGGGGTGTCGTAc                            >  2:1666000/1‑150 (MQ=255)

                                                                                                                                 ggTCGGAAAACAAAGAGGAAAG‑GG‑GGGGGGGG‑CTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATACGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGTTGGGGTGTCGTACCacac                       >  2:794679/1‑150 (MQ=255)

                                                                                                                                    cGGAAAACAAAGAGGAAAG‑GG‑GGGGGGGG‑CAAAACGGCAGGGAAGGCCCCCCCGGAAAGCGGGCGGCAGAAGGAAACAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGTTGGGGTGTCGAACCACACttt                    >  2:1612037/1‑150 (MQ=255)

                                                                                                                                     ggAAAACAAAGAGGAAAG‑GG‑GGGGGGGG‑CTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAAACAGAATTACCAGGTCAGACGGGCTGCAAGTTGAATACCGTTAAAAACATCGGTTGGGGTGTCGTACCACACTTTa                   >  2:634786/1‑150 (MQ=255)

                                                                                                                                      gAAAACAAAGAGGAAAG‑GG‑GGGGGGGG‑CTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGTTGGGGTGTCGTACCACACTTTAc                  >  1:2204656/1‑150 (MQ=255)

                                                                                                                                       aaaaCAAAGAGGAAAG‑GG‑GGGGGGGG‑CTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGTTGGGGTGTCGTACCACACTTTAcc                 >  2:2045300/1‑150 (MQ=255)

                                                                                                                                       aaaaCAAAGAGGAAAG‑GG‑GGGGGGGG‑CTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGTTGGGGTGTCGTACCACACTTTAcc                 >  1:291355/1‑150 (MQ=255)

                                                                                                                                           cAAAGAGGAAAG‑GG‑GGGGGGGG‑CTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGTTGGGGTGTCGTACCACACTTTACCTGcc             >  1:496104/1‑150 (MQ=255)

                                                                                                                                               gagGAAAG‑GG‑GGGGGGGG‑CTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGTTGGGGTGTCGTACCACACTTTACCTGCCGTCa         >  1:3089419/1‑150 (MQ=255)

                                                                                                                                               gagGAAAG‑GG‑GGGGGGGG‑CTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGTTGGGGTGTCGTACCACACTTTACCTGCCGTCa         >  1:3088490/1‑150 (MQ=255)

                                                                                                                                                     a‑‑GG‑GGGGGGGG‑CTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGTTGGGGTGTCGTACCACACTTTACCTGCCGTCAGCCCgag  >  2:1461725/1‑150 (MQ=255)

CTTCCGGAGTACTGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑‑GG‑GGGGGGGG‑CTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGTTGGGGTGTCGTACCACACTTTACCTGCCGTCAGCCCGAG  >  CP009273/375319‑375617

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

TGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGTTGGGGTGTCGTACCACACTTTACCTGCCGTCA  >  CP009273/375331‑375610

TGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑‑GGGGGGGGGGgc                                                                                                                                    >  SRR19973061.1253910/1‑148 (MQ=60)

             CCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑‑GGGGGGGGGGCTAATCGGCAGGGAA                                                                                                                       >  SRR19973061.408114/1‑150 (MQ=60)

                 aacatttgattgaaatatactattggttgttatacttttttaaaattcaaacccccatattttaaataacactttatAATAAAATTTTTCCGTTGAATGTGGACGCAAAACAACCACCAAA‑‑GGGGGGGGGGCTAATCGGCAGGGAAGGCC                                                                                                                   <  SRR19973061.3694811/73‑1 (MQ=26)

                   CATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGC                                                                                                                 >  SRR19973061.1227124/1‑150 (MQ=60)

                      acataaatcaatatattcttgttttttttttattttttaccttaacccccccattttttatttccctctccataaaaatttttccccgtttttggggcccgcaaccccccccccaccgGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCC                                                                                                                <  SRR19973061.1314224/32‑1 (MQ=60)

                       TGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAG‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCC                                                                                                              >  SRR19973061.1019965/1‑150 (MQ=60)

                       TGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAG‑GGGGGGGGGGCTAATAGGCAGGGAAGGCGGCCCC                                                                                                              >  SRR19973061.1019954/1‑150 (MQ=60)

                        GCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAG‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCG                                                                                                             >  SRR19973061.2516094/1‑150 (MQ=60)

                              AATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAG‑GGGGGGGGGGCTAATCAGAAGGGAAGGACGCCCCGGATAGC                                                                                                       >  SRR19973061.2915245/1‑150 (MQ=60)

                                    CTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAG‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGG                                                                                                 >  SRR19973061.943659/1‑150 (MQ=60)

                                    CTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAG‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGG                                                                                                 >  SRR19973061.3649506/1‑150 (MQ=60)

                                    CTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAG‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGG                                                                                                 >  SRR19973061.1100671/1‑150 (MQ=60)

                                     TATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAG‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGC                                                                                                >  SRR19973061.3382402/1‑150 (MQ=60)

                                     TATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAG‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGC                                                                                                >  SRR19973061.3382464/1‑150 (MQ=60)

                                     TATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAG‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGC                                                                                                >  SRR19973061.1065992/1‑150 (MQ=60)

                                      cctccccctcactacaacctcccatccccccccccccctttctcactccccccccccacccccctcccccccccccttccccccccaccccccccccccccgGGGGGGGGGGCTACGCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGC                                                                                                <  SRR19973061.180168/48‑1 (MQ=60)

                                        tttttttataaaaccccaccccccacccacccttaataataaccccttttaaactcatctctccccttttcttccccccccaccaccccccccccccgggGGGGGGGGGGCAAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAG                                                                                              <  SRR19973061.1297449/50‑1 (MQ=60)

                                           GGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAG‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGG                                                                                          >  SRR19973061.2092798/1‑150 (MQ=60)

                                                ATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAG‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCA                                                                                     >  SRR19973061.1833229/1‑150 (MQ=60)

                                                ATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAG‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCA                                                                                     >  SRR19973061.44866/1‑150 (MQ=60)

                                                   ttcacactcacacaccaaacccccctatattatatcacacttcatactaaactttcccctgtttccctccccccccccccccccccaccGGGGGGGGGCCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGA                                                                                   <  SRR19973061.409311/61‑1 (MQ=60)

                                                          TCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAG‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAG                                                                           >  SRR19973061.2483987/1‑150 (MQ=60)

                                                           cccacccacccccccccccatactaaacccccccccaccaccacttcccccctccctcctcccccccccccaccccccccgcggcGGGGGGATAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAG                                                                           <  SRR19973061.2374087/65‑1 (MQ=60)

                                                           ccacccccccccccccaacacacaccccccccccccccccccctccccccccccccccccccccccccccccccccccccccggcGGGGGCCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAG                                                                           <  SRR19973061.462780/65‑1 (MQ=60)

                                                            AATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGG                                                                          >  SRR19973061.1302385/1‑150 (MQ=60)

                                                            AATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAG‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGT                                                                         >  SRR19973061.2110712/1‑150 (MQ=60)

                                                            AATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAG‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGT                                                                         >  SRR19973061.2815053/1‑150 (MQ=60)

                                                            AATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAG‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGAAAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGT                                                                         >  SRR19973061.2112186/1‑150 (MQ=60)

                                                             ATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCA                                                                       >  SRR19973061.1184159/1‑150 (MQ=60)

                                                               accatcccccccaatctaacatccccctccataaaaacctttctcccttcctcttgctctcaaccccccccccacccgcGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCA                                                                       <  SRR19973061.3716775/71‑1 (MQ=60)

                                                                CATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAG‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGA                                                                     >  SRR19973061.881604/1‑150 (MQ=60)

                                                                CATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGAC                                                                    >  SRR19973061.3161314/1‑150 (MQ=60)

                                                                  ccccccccccaccccaacccccccccccccccccccctccccccccccctcccccccccccccccccccccccccccgcccccGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGAC                                                                    <  SRR19973061.742318/67‑1 (MQ=60)

                                                                   ACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAG‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGACTCAGAATTTCCAGGTCAGACGG                                                                  >  SRR19973061.2669580/1‑150 (MQ=60)

                                                                       ccccccccccccccccccctcccccccccccccccccccccccctcccccccccccccccccccccccccccccgcccGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCT                                                               <  SRR19973061.304104/72‑1 (MQ=60)

                                                                       CCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAG‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTG                                                              >  SRR19973061.2361033/1‑150 (MQ=60)

                                                                       CCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGC                                                             >  SRR19973061.1720528/1‑150 (MQ=60)

                                                                        CCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAG‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGC                                                             >  SRR19973061.952894/1‑150 (MQ=60)

                                                                        CCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAG‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGC                                                             >  SRR19973061.952481/1‑150 (MQ=60)

                                                                            TAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAG‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGT                                                         >  SRR19973061.3511063/1‑150 (MQ=60)

                                                                             AGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAG‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTT                                                        >  SRR19973061.2924199/1‑150 (MQ=60)

                                                                               TATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAG‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGC                                                      >  SRR19973061.1483738/1‑150 (MQ=60)

                                                                                  ATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAG‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGA                                                   >  SRR19973061.1194081/1‑150 (MQ=60)

                                                                                    AACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAG‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAAACAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACC                                                 >  SRR19973061.3487400/1‑150 (MQ=60)

                                                                                    AACACTGGAGAATAAAATTTATCCGGTGAATGTGGCCGGAAAACAAAGAGGAAAG‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACC                                                 >  SRR19973061.3129163/1‑150 (MQ=60)

                                                                                           ccccccccccccccccccccccccccccccccccccccccccccccccccccccccctGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAA                                           <  SRR19973061.443587/92‑1 (MQ=60)

                                                                                           ccaccccccaccccccccccctccccccccccccccccccccccccccgcccccgcccGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAA                                           <  SRR19973061.442931/92‑1 (MQ=60)

                                                                                               ATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAG‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCATAATTTACAGGTCAAACGGGCTGCAAGTTGCAGAACGTTAAAATCAT                                      >  SRR19973061.1455320/1‑150 (MQ=60)

                                                                                                  AAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAG‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGG                                   >  SRR19973061.1190901/1‑150 (MQ=60)

                                                                                                  AAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAG‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGG                                   >  SRR19973061.3542236/1‑150 (MQ=60)

                                                                                                   AATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAG‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGT                                  >  SRR19973061.434285/1‑150 (MQ=60)

                                                                                                     TTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAG‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGTTG                                >  SRR19973061.2761745/1‑150 (MQ=60)

                                                                                                      TTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑‑GGGGGGGGGGgcaaaacgccagggaaggcccccccgaaaagcggggggcaaaagaaaacaaaattaccaggacaaacggccgccaagttccaacccgtacaaaacaacggttgg                              >  SRR19973061.2758517/1‑46 (MQ=60)

                                                                                                        accccctttcacgtgcccccaaccccccccccaccgcGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGTGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGTTGGG                              <  SRR19973061.2422709/113‑1 (MQ=60)

                                                                                                                          GAAAACAAAGAGGAAAG‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGTTGGGGTGTCGTACCACACTTTAC           >  SRR19973061.2210196/1‑150 (MQ=60)

                                                                                                                           AAAACAAAGAGGAAAG‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGTTGGGGTGTCGTACCACACTTTACC          >  SRR19973061.292136/1‑150 (MQ=60)

                                                                                                                               CAAAGAGGAAAG‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGTTGGGGTGTCGTACCACACTTTACCTGCC      >  SRR19973061.497415/1‑150 (MQ=60)

                                                                                                                                 AAGAGGAAA‑‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGTTGGGGTGTCGTACCACACTTTACCTGCCGTC   >  SRR19973061.540565/1‑150 (MQ=60)

                                                                                                                                   GAGGAAAG‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGTTGGGGTGTCGTACCACACTTTACCTGCCGTCA  >  SRR19973061.3096451/1‑150 (MQ=60)

                                                                                                                                   GAGGAAAG‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGTTGGGGTGTCGTACCACACTTTACCTGCCGTCA  >  SRR19973061.3097384/1‑150 (MQ=60)

TGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGTTGGGGTGTCGTACCACACTTTACCTGCCGTCA  >  CP009273/375331‑375610

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`