Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F534 I1 R1 1235 230.2 7611948 95.1% 7238962 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009273 535,073 C→A I157I (ATC→ATA)  allB → allantoinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273535,0730CA100.0% 609.2 / NA 186I157I (ATC→ATA) allBallantoinase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (91/95);  total (91/95)

Only 100 of 188 total aligned reads displayed.

TTCGATGCCGCTAAAGGCAAGCTGACTATTGATGCGGCACAACTCGGTGGCCTGGTGTCTTACAACATCGACCGTCTGCATGAGCTGGATGAAGTGGGCGTTGTCGGCTTCAAATGCTTCGTTGCGACCTGTGGCGATCGCGGTATCGACAACGACTTCCGTGATGTAAACGACTGGCAGTTCTTCAAAGGTGCGCAGAAGCTGGGCGAACTGGGTCAGCCGGTGCTGGTGCACTGCGAAAACGCGCTGATTTGTGACGAACTGGGCGAAGAAGCGAAGCGTGAAGGTCGCGT  >  CP009273/534927‑535219
                                                                                                                                                  |                                                                                                                                                  
ttCGATGCCGCTAAAGGCAAGCTGACTATTGATGCGGCACAACTCGGTGGCCTGGTGTCTTACAACATCGACCGTCTGCATGAGCTGGATGAAGTGGGCGTTGTCGGCTTCAAATGCTTCGTTGCGACCTGTGGCGATCGCGGTATAGAc                                                                                                                                                 >  2:1062804/1‑150 (MQ=255)
   gATGCCGCTAAAGGCAAGCTGACTATTGATGCGGCACAACTCGGTGGCCTGGTGTCTTACAACATCGACCGTCTGCATGAGCTGGATGAAGTGGGCGTTGTCGGCTTCAAATGCTTCGTTGCGACCTGTGGCGATCGCGGTATAGACAAc                                                                                                                                              >  1:818342/1‑150 (MQ=255)
   gATGCCGCTAAAGGCAAGCTGACTATTGATGCGGCACAACTCGGTGGCCTGGTGTCTTACAACATCGACCGTCTGCATGAGCTGGATGAAGTGGGCGTTGTCGGCTTCAAATGCTTCGTTGCGACCTGTGGCGATCGCGGTATAGACAAc                                                                                                                                              >  1:22537/1‑150 (MQ=255)
     tGCCGCTAAAGGCAAGCTGACTATTGATGCGGCACAACTCGGTGGCCTGGTGTCTTACAACATCGACCGTCTGCATGAGCTGGATGAAGTGGGCGTTGTCGGCTTCAAATGCTTCGTTGCGACCTGTGGCGATCGCGGTATAGACAACGa                                                                                                                                            >  1:1875212/1‑150 (MQ=255)
          cTAAAGGCAAGCTGACTATTGATGCGGCACAACTCGGTGGCCTGGTGTCTTACAACATCGACCGTCTGCATGAGCTGGATGAGGTGGGCGTTGTCGGCTTCAAATGCTTCGTTGCGACCTGTGGCGATCGCGGTATAGACAACGACTTcc                                                                                                                                       >  2:2411558/1‑150 (MQ=255)
          cTAAAGGCAAGCTGACTATTGATGCGGCACAACTCGGTGGCCTGGTGTCTTACAACATCGACCGTCTGCATGAGCTGGATGAAGTGGGCGTTGTCGGCTTCAAATGCTTCGTTGCGACCTGTGGCGATCGCGGTATAGACAACGACTTcc                                                                                                                                       >  2:504060/1‑150 (MQ=255)
          cTAAAGGCAAGCTGACTATTGATGCGGCACAACTCGGTGGCCTGGTGTCTTACAACATCGACCGTCTGCATGAGCTGGATGAAGTGGGCGTTGTCGGCTTCAAATGCTTCGTTGCGACCTGTGGCGATCGCGGTATAGACAACGACTTcc                                                                                                                                       >  2:1692966/1‑150 (MQ=255)
          cTAAAGGCAAGCTGACTATTGATGCGGCACAACTCGGTGGCCTGGTGTCTTACAACATCGACCGTCTGCATGAGCTGGATGAAGTGGGCGTTGTCGGCTTCAAATGCTTCGTTGCGACCTGTGGCGATCGCGGTATAGACAACGACTTcc                                                                                                                                       >  1:527967/1‑150 (MQ=255)
          cTAAAGGCAAGCTGACTATTGATGCGGCACAACTCGGTGGCCTGGTGTCTTACAACATCGACCGTCTGCATGAGCTGGATGAAGTGGGCGTTGTCGGCTTCAAATGCTTCGTTGCGACCTGTGGCGATCGCGGTATAGACAACGACTTcc                                                                                                                                       >  2:3121359/1‑150 (MQ=255)
           tAAAGGCAAGCTGACTATTGATGCGGCACAACTCGGTGGCCTGGTGTCTTACAACATCGACCGTCTGCATGAGCTGGATGAAGTGGGCGTTGTCGGCTTCAAATGCTTCGTTGCGACCTGTGGCGATCGCGGTATAGACAACGACTTCCg                                                                                                                                      <  1:1084514/150‑1 (MQ=255)
           tAAAGGCAAGCTGACTATTGATGCGGCACAACTCGGTGGCCTGGTGTCTTACAACATCGACCGTCTGCATGAGCTGGATGAAGTGGGCGTTGTCGGCTTCAAATGCTTCGTTGCGACCTGTGGCGATCGCGGTATAGACAACGACTTCCg                                                                                                                                      >  2:2867628/1‑150 (MQ=255)
             aaGGCAAGCTGACTATTGATGCGGCACAACTCGGTGGCCTGGTGTCTTACAACATCGACCGTCTGCATGAGCTGGATGAAGTGGGCGTTGTCGGCTTCAAATGCTTCGTTGCGACCTGTGGCGATCGCGGTATAGACAACGACTTCCGTg                                                                                                                                    >  1:274732/1‑150 (MQ=255)
             aaGGCAAGCTGACTATTGATGCGGCACAACTCGGTGGCCTGGTGTCTTACAACATCGACCGTCTGCATGAGCTGGATGAAGTGGGCGTTGTCGGCTTCAAATGCTTCGTTGCGACCTGTGGCGATCGCGGTATAGACAACGACTTCCGTg                                                                                                                                    >  1:3744257/1‑150 (MQ=255)
               ggCAAGCTGACTATTGATGCGGCACAACTCGGTGGCCTGGTGTCTTACAACATCGACCGTCTGCATGAGCTGGATGAAGTGGGCGTTGTCGGCTTCAAATGCTTCGTTGCGACCTGTGGCGATCGCGGTATAGACAACGACTTCCGTGAt                                                                                                                                  >  2:602421/1‑150 (MQ=255)
               ggCAAGCTGACTATTGATGCGGCACAACTCGGTGGCCTGGTGTCTTACAACATCGACCGTCTGCATGAGCTGGATGAAGTGGGCGTTGTCGGCTTCAAATGCTTCGTTGCGACCTGTGGCGATCGCGGTATAGACAACGACTTCCGTGAt                                                                                                                                  >  2:3303659/1‑150 (MQ=255)
                gCAAGCTGACTATTGATGCGGCACAACTCGGTGGCCTGGTGTCTTACAACATCGACCGTCTGCATGAGCTGGATGAAGTGGGCGTTGTCGGCTTCAAATGCTTCGTTGCGACCTGTGGCGATCGCGGTATAGACAACGACTTCCGTGATg                                                                                                                                 >  2:1317155/1‑150 (MQ=255)
                gCAAGCTGACTATTGATGCGGCACAACTCGGTGGCCTGGTGTCTTACAACATCGACCGTCTGCATGAGCTGGATGAAGTGGGCGTTGTCGGCTTCAAATGCTTCGTTGCGACCTGTGGCGATCGCGGTATAGACAACGACTTCCGTGATg                                                                                                                                 >  2:2088981/1‑150 (MQ=255)
                   aGCTGACTATTGATGCGGCACAACTCGGTGGCCTGGTGTCTTACAACATCGACCGTCTGCATGAGCTGGATGAAGTGGGCGTTGTCGGCTTCAAATGCTTCGTTGCGACCTGTGGCGATCGCGGTATAGACAACGACTTCCGTGATGTaa                                                                                                                              <  2:1118293/150‑1 (MQ=255)
                    gCTGACTATTGATGCGGCACAACTCGGTGGCCTGGTGTCTTACAACATCGACCGTCTGCATGAGCTGGATGAAGTGGGCGTTGTCGGCTTCAAATGCTTCGTTGCGACCTGTGGCGATCGCGGTATAGACAACGACTTCCGTGATGTaaa                                                                                                                             <  2:1151901/150‑1 (MQ=255)
                    gCTGACTATTGATGCGGCACAACTCGGTGGCCTGGTGTCTTACAACATCGACCGTCTGCATGAGCTGGATGAAGTGGGCGTTGTCGGCTTCAAATGCTTCGTTGCGACCTGTGGCGATCGCGGTATAGACAACGACTTCCGTGATGTaaa                                                                                                                             >  1:796859/1‑150 (MQ=255)
                     cTGACTATTGATGCGGCACAACTCGGTGGCCTGGTGTCTTACAACATCGACCGTCTGCATGAGCTGGATGAAGTGGGCGTTGTCGGCTTCAAATGCTTCGTTGCGACCTGTGGCGATCGCGGTATAGACAACGACTTCCGTGATGTAAAc                                                                                                                            >  2:1409439/1‑150 (MQ=255)
                     cTGACTATTGATGCGGCACAACTCGGTGGCCTGGTGTCTTACAACATCGACCGTCTGCATGAGCTGGATGAAGTGGGCGTTGTCGGCTTCAAATGCTTCGTTGCGACCTGTGGCGATCGCGGTATAGACAACGACTTCCGTGATGTAAAc                                                                                                                            <  1:3569588/150‑1 (MQ=255)
                           aTTGATGCGGCACAACTCGGTGGCCTGGTGTCTTACAACATCGACCGTCTGCATGAGCTGGATGAAGTGGGCGTTGTCGGCTTCAAATGCTTCGTTGCGACCTGTGGCGATCGCGGTATAGACAACGACTTCCGTGATGTAAACGACTgg                                                                                                                      >  1:1019918/1‑150 (MQ=255)
                                tGCGGCACAACTCGGTGGCCTGGTGTCTTACAACATCGACCGTCTGCATGAGCTGGATGAAGTGGGCGTTGTCGGCTTCAAATGCTTCGTTGCGACCTGTGGCGATCGCGGTATAGACAACGACTTCCGTGATGTAAACGACTGGCAGtt                                                                                                                 <  2:2301624/150‑1 (MQ=255)
                                      acaACTCGGTGGCCTGGTGTCTTACAACATCGACCGTCTGCATGAGCTGGATGAAGTGGGCGTTGTCGGCTTCAAATGCTTCGTTGCGACCTGTGGCGATCGCGGTATAGACAACGACTTCCGTGATGTAAACGACCGGCAGTTCTTCaa                                                                                                           >  1:3622947/1‑150 (MQ=255)
                                       caACTCGGTGGCCTGGTGTCTTACAACATCGACCGTCTGCATGAGCTGGATGATGTGGGCGTTGTCGGCTTCAAATGCTTCGTTGCGACCTGTGGCGATCGCGGTATAGACAACGACTTCCGTGATGTAAACGACTGGCAGTTCTTCaaa                                                                                                          <  1:2918754/150‑1 (MQ=255)
                                        aaCTCGGTGGCCTGGTGTCTTACAACATCGACCGTCTGCATGAGCTGGATGAAGTGGGCGTTGTCGGCTTCAAATGCTTCGTTGCGACCTGTGGCGATCGCGGTATAGACAACGACTTCCGTGATGTAAACGACTGGCAGTTCTTCAAAg                                                                                                         >  2:2539142/1‑150 (MQ=255)
                                         aCTCGGTGGCATGGTGTTTTACAACATCGACCGTTGGCATGAGCTGGATGATGTGGGTGTTGTCGGCTTAAAATGATTCGTTGCGACCTGTGGCGATCGCGGTATAGACAACGACTTCCGTGATTTAAACGACTGGCAGTTCTTCAAAgg                                                                                                        <  1:2338052/150‑1 (MQ=255)
                                          cTCGGTGGCCTGGTGTCTTACAACATCGACCGTCTGCATGAGCTGGATGAAGTGGGCGTTGTCGGCTTCAAATGCTTCGTTGCGACCTGTGGCGATCGCGGTATAGACAACGACTTCCGTGATGTAAACGACTGGCAGTTCTTCAAAGGt                                                                                                       <  2:1944945/150‑1 (MQ=255)
                                           tCGGTGGCCTGGTGTCTTACAACATCGACCGTCTGCATGAGCTGGATGAAGTGGGCGTTGTCGGCTTCAAATGCTTCGTTGCGACCTGTGGCGATCGCGGTATAGACAACGACTTCCGTGATGTAAACGACTGGCAGTTCTTCAAAGGTg                                                                                                      >  1:1542989/1‑150 (MQ=255)
                                           tCGGTGGCCTGGTGTCTTACAACATCGACCGTCTGCATGAGCTGGATGAAGTGGGCGTTGTCGGCTTCAAATGCTTCGTTGCGACCTGTGGCGATCGCGGTATAGACAACGACTTCCGTGATGTAAACGACTGGCAGTTCTTCAAAGGTg                                                                                                      >  1:3438218/1‑150 (MQ=255)
                                               tGGCCTGGTGTCTTACAACATCGACCGTCTGCATGAGCTGGATGAAGTGGGCGTTGTCGGCTTCAAATGCTTCGTTGCGACCTGTGGCGATCGCGGTATAGACAACGACTTCCGTGATGTAAACGACTGGCAGTTCTTCAAAGGTGCGCa                                                                                                  <  1:3401204/150‑1 (MQ=255)
                                                 gCCTGGTGTCTTACAACATCGACCGTCTGCATGAGCTGGATGAAGTGGGCGTTGTCGGCTTCAAATGCTTCGTTGCGACCTGTGGCGATCGCGGTATAGACAACGACTTCCGTGATGTAAACGACTGGCAGTTCTTCAAAGGTGCGCAGa                                                                                                >  1:3239721/1‑150 (MQ=255)
                                                 gCCTGGTGTCTTACAACATCGACCGTCTGCATGAGCTGGATGAAGTGGGCGTTGTCGGCTTCAAATGCTTCGTTGCGACCTGTGGCGATCGCGGTATAGACAACGACTTCCGTGATGTAAACGACTGGCAGTTCTTCAAAGGTGCGCAGa                                                                                                >  1:2028623/1‑150 (MQ=255)
                                                  ccTGGTGTCTTACAACATCGACCGTCTGCATGAGCTGGATGAAGTGGGCGTTGTCGGCTTCAAATGCTTCGTTGCGACCTGTGGCGATCGCGGTATAGACAACGACTTCCGTGATGTAAACGACTGGCAGTTCTTCAAAGGTGCGCAGaa                                                                                               <  2:696965/150‑1 (MQ=255)
                                                  ccTGGTGTCTTACAACATCGACCGTCTGCATGAGCTGGATGAAGTGGGCGTTGTCGGCTTCAAATGCTTCGTTGCGACCTGTGGCGATCGCGGTATAGACAACGACTTCCGTGATGTAAACGACTGGCAGTTCTTCAAAGGTGCGCAGaa                                                                                               >  2:3463392/1‑150 (MQ=255)
                                                    tGGTGTCTTACAACATCGACCGTCTGCATGAGCTGGATGAAGTGGGCGTTGTCGGCTTCAAATGCTTCGTTGCGACCTGTGGCGATCGCGGTATAGACAACGACTTCCGTGATGTAAACGACTGGCAGTTCTTCAAAGGTGCGCAGAAGc                                                                                             >  1:1628780/1‑150 (MQ=255)
                                                     ggTGTCTTACAACATCGACCGTCTGCATGAGCTGGATGATGTGGGCGTTGTCGGCTTCAAATGCTTCGTTGCGACCTGTGGCGATCGCGGTATAGACAACGACTTCCGTGATGTAAACGACTGGCAGTTCTTCAAAGGTGCGCAGAAGCt                                                                                            <  1:3288753/150‑1 (MQ=255)
                                                     ggTGTCTTACAACATCGACCGTCTGCATGAGCTGGATGAAGTGGGCGTTGTCGGCTTCAAATGCTTCGTTGCGACCTGTGGCGAGCGCGGTATAGACAACGACTTCCGTGATGTAAACGACTGGCAGTTCTTCAAAGGTGCGCAGAAGCt                                                                                            >  2:2057088/1‑150 (MQ=255)
                                                      gtgtCTTACAACATCGACCGTCTGCATGAGCTGGATGAAGTGGGCGTTGTCGGCTTCAAATGCTTCGTTGCGACCTGTGGCGATCGCGGTATAGACAACGACTTCCGTGATGTAAACGACTGGCAGTTCTTCAAAGGTGCGCAGAAGCTg                                                                                           >  1:2003552/1‑150 (MQ=255)
                                                               aacaTCGACCGTCTGCATGAGCTGGATGAAGTGGGCGTTGTCGGCTTCAAATGCTTCGTTGCGACCTGTGGCGATCGCGGTATAGACAACGACTTCCGTGATGTAAACGACTGGCAGTTCTTCAAAGGTGCGCAGAAGCTGGGCGAACTg                                                                                  <  2:633637/150‑1 (MQ=255)
                                                                 caTCGACCGTCTGCATGAGCTGGATGAAGTGGGCGTTGTCGGCTTCAAATGCTTCGTTGCGACCTGTGGCGATCGCGGTATAGACAACGACTTCCGTGATGTAAACGACTGGCAGTTCTTCAAAGGTGCGCAGAAGCTGGGCGAACTggg                                                                                >  1:1866076/1‑150 (MQ=255)
                                                                  aTCGACCGTCTGCATGAGCTGGATGAAGTGGGCGTTGTCGGCTTCAAATGCTTCGTTGCGACCTGTGGCGATCGCGGTATAGACAACGACTTCCGTGATGTAAACGACTGGCAGTTCTTCAAAGGTGCGCAGAAGCTGGGCGAACTGGGt                                                                               >  1:649547/1‑150 (MQ=255)
                                                                     gACCGTCTGCATGAGCTGGATGAAGTGGGCGTTGTCGGCTTCAAATGCTTCGTTGCGACCTGTGGCGATCGCGGTATAGACAACGACTTCCGTGATGTAAACGACTGGCAGTTCTTCAAAGGTGCGCAGAAGCTGGGCGAACTGGGTCAg                                                                            <  1:1170101/150‑1 (MQ=255)
                                                                           cTGCATGAGCTGGATGAAGTGGGCGTTGTCGGCTTCAAATGCTTCGTTGCGACCTGTGGCGATCGCGGTATAGACAACGACTTCCGTGATGTAAACGACTGGCAGTTCTTCAAAGGTGCGCAGAAGCTGGGCGAACTGGGTCAGCCGGTg                                                                      >  2:2476530/1‑150 (MQ=255)
                                                                            tGCATGAGCTGGATGAAGTGGGCGTTGTCGGCTTCAAATGCTTCGTTGCGACCTGTGGCGATCGCGGTATAGACAACGACTTCCGTGATGTAAACGACTGGCAGTTCTTCAAAGGTGCGCAGAAGCTGGGCGAACTGGGTCAGCCGGTGc                                                                     >  2:2460211/1‑150 (MQ=255)
                                                                              cATGAGCTGGATGAAGTGGGCGTTGTCGGCTTCAAATGCTTCGTTGCGACCTGTGGCGATCGCGGTATAGACAACGACTTCCGTGATGTAAACGACTGGCAGTTCTTCAAAGGTGCGCAGAAGCTGGGCGAACTGGGTCAGCCGGTGCTg                                                                   <  1:677672/150‑1 (MQ=255)
                                                                              cATGAGCTGGATGAAGTGGGCGTTGTCGGCTTCAAATGCTTCGTTGCGACCTGTGGCGATCGCGGTATAGACAACGACTTCCGTGATGTAAACGACTGGCAGTTCTTCAAAGGTGCGCAGAAGCTGGGCGAACTGGGTCAGCCGGTGCTg                                                                   >  1:3182503/1‑150 (MQ=255)
                                                                              cATGAGCTGGATGAAGTGGGCGTTGTCGGCTTCAAATGCTTCGTTGCGACCTGTGGCGATCGCGGTATAGACAACGACTTCCGTGATGTAAACGACTGGCAGTTCTTCAAAGGTGCGCAGAAGCTGGGCGAACTGGGTCAGCCGGTGCTg                                                                   <  2:2616337/150‑1 (MQ=255)
                                                                               aTGAGCTGGATGAAGTGGGCGTTGTCGGCTTCAAATGCTTCGTTGCGACCTGTGGCGATCGCGGTATAGACAACGACTTCCGTGATGTAAACGACTGGCAGTTCTTCAAAGGTGCGCAGAAGCTGGGCGAACTGGGTCAGCCGGTGCTgg                                                                  >  2:860450/1‑150 (MQ=255)
                                                                                 gAGCTGGATGAAGTGGGCGTTGTCGGCTTCAAATGCTTCGTTGCGACCTGTGGCGATCGCGGTATAGACAACGACTTCCGTGATGTAAACGACTGGCAGTTCTTCAAAGGTGCGCAGAAGCTGGGCGAACTGGGTCAGCCGGTGCTGGTg                                                                >  1:2381223/1‑150 (MQ=255)
                                                                                 gAGCTGGATGAAGTGGGCGTTGTCGGCTTCAAATGCTTCGTTGCGACCTGTGGCGATCGCGGTATAGACAACGACTTCCGTGATGTAAACGACTGGCAGTTCTTCAAAGGTGCGCAGAAGCTGGGCGAACTGGGTCAGCCGGTGCTGGTg                                                                <  1:1710297/150‑1 (MQ=255)
                                                                                      ggATGAAGTGGGCGTTGTCGGCTTCAAATGCTTCGTTGCGACCTGTGGCGATCGCGGTATAGACAACGACTTCCGTGATGTAAACGACTGGCAGTTCTTCAAAGGTGCGCAGAAGCTGGGCGAACTGGGTCAGCCGGTGCTGGTGCACTg                                                           <  2:1875212/150‑1 (MQ=255)
                                                                                      ggATGAAGTGGGCGTTGTCGGCTTCAAATGCTTCGTTGCGACCTGTGGCGATCGCGGTATAGACAACGACTTCCGTGATGTAAACGACTGGCAGTTCTTCAAAGGTGCGCAGAAGCTGGGCGAACTGGGTCAGCCGGTGCTGGTGCACTg                                                           >  2:2710702/1‑150 (MQ=255)
                                                                                       gATGAAGTGGGCGTTGTCGGCTTCAAATGCTTCGTTGCGACCTGTGGCGATCGCGGTATAGACAACGACTTCCGTGATGTAAACGACTGGCAGTTCTTCAAAGGTGCGCAGAAGCTGGGCGAACTGGGTCAGCCGGTGCTGGTGCACTGc                                                          >  2:1498581/1‑150 (MQ=255)
                                                                                        aTGAAGTGGGCGTTGTCGGCTTCAAATGCTTCGTTGCGACCTGTGGCGATCGCGGTATAGACAACGACTTCCGTGATGTAAACGACTGGCAGTTCTTCAAAGGTGCGCAGAAGCTGGGCGAACTGGGTCAGCCGGTGCTGGTGCACTGCg                                                         >  1:1425464/1‑150 (MQ=255)
                                                                                        aTGAAGTGGGCGTTGTCGGCTTCAAATGCTTCGTTGCGACCTGTGGCGATCGCGGTATAGACAACGACTTCCGTGATGTAAACGACTGGCAGTTCTTCAAAGGTGCGCAGAAGCTGGGCGAACTGGGTCAGCCGGTGCTGGTGCACTGCg                                                         <  2:2696645/150‑1 (MQ=255)
                                                                                             gTGGGCGTTGTCGGCTTCAAATGCTTCGTTGCGACCTGTGGCGATCGCGGTATAGACAACGACTTCCGTGATGTAAACGACTGGCAGTTCTTCAAAGGTGCGCAGAAGCTGGGCGAACTGGGTCAGCCGGTGCTGGTGCACTGCGAAAAc                                                    >  2:364899/1‑150 (MQ=255)
                                                                                             gTGGGCGTTGTCGGCTTCAAATGCTTCGTTGCGACCTGTGGCGATCGCGGTATAGACAACGACTTCCGTGATGTAAACGACTGGCAGTTCTTCAAAGGTGCGCAGAAGCTGGGCGAACTGGGTCAGCCGGTGCTGGTGCACTGCGAAAAc                                                    <  2:3486755/150‑1 (MQ=255)
                                                                                             gTGGGCGTTGTCGGCTTCAAATGCTTCGTTGCGACCTGTGGCGATCGCGGTATAGACAACGACTTCCGTGATGTAAACGACTGGCAGTTCTTCAAAGGTGCGCAGAAGCTGGGCGAACTGGGTCAGCCGGTGCTGGTGCACTGCGAAAAc                                                    <  1:3251066/150‑1 (MQ=255)
                                                                                             gTGGGCGGTGTCGGCTTCAAATGCTTCGTTGCGACCTGTGGCGATCGCGGTATAGACAACGACTTCCGTGATGTAAACGACTGGCAGTTCTTCAAAGGTGCGCAGAAGCTGGGCGAACTGGGTCAGCCGGTGCTGGTGCACTGCGAAAAc                                                    <  1:1061342/150‑1 (MQ=255)
                                                                                              tGGGCGTTGTCGGCTTCAAATGCTTCGTTGCGACCTGTGGCGATCGCGGTATAGACAACGACTTCCGTGATGTAAACGACTGGCAGTTCTTCAAAGGTGCGCAGAAGCTGGGCGAACTGGGTCAGCCGGTGCTGGTGCACTGCGAAAAcg                                                   <  1:3428502/150‑1 (MQ=255)
                                                                                               gggCGTTGTCGGCTTCAAATGCTTCGTTGCGACCTGTGGCGATCGCGGTATAGACAACGACTTCCGTGATGTAAACGACTGGCAGTTCTTCAAAGGTGCGCAGAAGCTGGGCGAACTGGGTCAGCCGGTGCTGGTGCACTGCGAAAAcgc                                                  <  1:1422637/150‑1 (MQ=255)
                                                                                                   gtggtgggCTTCATAGGTTTCGTTGCGACCTGTGGTGATCGCGGTATAGACAACGACTTCCGTGATGTAAACGACTGGCAGTTCTTCAAAGGTGCGCAGAAGCTGGGCGAACTGGGTCAGCCGGTGCTGGTGCACTGCGAAAACGCGCTg                                              <  1:646633/144‑1 (MQ=255)
                                                                                                   gTTGTCGGCTTCAAATGCTTCGTTGCGACCTGTGGCGATCGCGGTATAGACAACGACTTCCGTGATGTAAACGACTGGCAGTTCTTCAAAGGTGCGCAGAAGCTGGGCGAACTGGGTCAGCCGGTGCTGGTGCACTGCGAAAACGCGCTg                                              >  2:429300/1‑150 (MQ=255)
                                                                                                   gTTGTCGGCTTCAAATGCTTCGTTGCGACCTGTGGCGATCGCGGTATAGACAACGACTTCCGTGATGTAAACGACTGGCAGTTCTTCAAAGGTGCGCAGAAGCTGGGCGAACTGGGTCAGCCGGTGCTGGTGCACTGCGAAAACGCGCTg                                              <  1:2695598/150‑1 (MQ=255)
                                                                                                    ttGTCGGCTTCAAATGCTTCGTTGCGACCTGTGGCGATCGCGGTATAGACAACGACTTCCGTGATGTAAACGACTGGCAGTTCTTCAAAGGTGCGCAGAAGCTGGGCGAACTGGGTCAGCCGGTGCTGGTGCACTGCGAAAACGCGCTGa                                             >  2:535908/1‑150 (MQ=255)
                                                                                                       tCGGCTTCAAATGCTTCGTTGCGACCTGTGGCGATCGCGGTATAGACAACGACTTCCGTGATGTAAACGACTGGCAGTTCTTCAAAGGTGCGCAGAAGCTGGGCGAACTGGGTCAGCCGGTGCTGGTGCACTGCGAAAACGCGCTGAttt                                          <  1:2568840/150‑1 (MQ=255)
                                                                                                          gCTTCAAATGCTTCGTTGCGACCTGTGGCGATCGCGGTATAGACAACGACTTCCGTGATGTAAACGACTGGCAGTTCTTCAAAGGTGCGCAGAAGCTGGGCGAACTGGGTCAGCCGGTGCTGGTGCACTGCGAAAACGCGCTGATTtgtg                                       <  2:3527287/150‑1 (MQ=255)
                                                                                                            ttCAAATGTTTCGTTGCGACCTGTGGCGATCGCGGTATAGACAACGACTTCCGTGATGTAAACGACTGGCAGTTCTTCAAAGGTGCGCAGAAGCTGGGCGAACTGGGTCAGCCGGTGCTGGTGCACTGCGAAAACGCGCTGATTTGTGAc                                     <  1:1312284/150‑1 (MQ=255)
                                                                                                            ttCAAATGCTTCGTTGCGACCTGTGGCGATCGCGGTATAGACAACGACTTCCGTGATGTAAACGACTGGCAGTTCTTCAAAGGTGCGCAGAAGCTGGGCGAACTGGGTCAGCCGGTGCTGGTGCACTGCGAAAACGCGCTGATTTGTGAc                                     >  2:769044/1‑150 (MQ=255)
                                                                                                            ttCAAATGCTTCGTTGCGACCTGTGGCGATCGCGGTATAGACAACGACTTCCGTGATGTAAACGACTGGCAGTTCTTCAAAGGTGCGCAGAAGCTGGGCGAACTGGGTCAGCCGGTGCTGGTGCACTGCGAAAACGCGCTGATTTGTGAc                                     >  1:251732/1‑150 (MQ=255)
                                                                                                                    cTTCGTTGCGACCTGTGGCGATCGCGGTATAGACAACGACTTCCGTGATGTAAACGACTGGCAGTTCTTCAAAGGTGCGCAGAAGCTGGGCGAACTGGGTCAGCCGGTGCTGGTGCACTGCGAAAACGCGCTGATTTGTGACGAACTggg                             >  2:2069805/1‑150 (MQ=255)
                                                                                                                     ttCGTTGCGTCCTGTGGGGATCGCGGTATATACAACGATTTCGGTTATGTAAACGACTGGCAGTTCTTCAAAGGTGCGCAGAAGCTGGGCGAACTGGGTCAGCCGGTGCTGGTGCACTGCGAAAACGCGCTGATTTGTGACGAACTGGGc                            <  1:1025853/150‑1 (MQ=255)
                                                                                                                     ttCGTTGCGACCTGTGGCGATCGCGGTATAGACAACGACTTCCGTGATGTAAACGACTGGCAGTTCTTCAAAGGTGCGCAGAAGCTGGGCGAACTGGGTCAGCCGGTGCTGGTGCACTGCGAAAACGCGCTGATTTGTGACGAACTGGGc                            >  2:3798085/1‑150 (MQ=255)
                                                                                                                     ttCGTTGCGACCTGTGGCGATCGCGGTATAGACAACGACTTCCGTGATGTAAACGACTGGCAGTTCTTCAAAGGTGCGCAGAAGCTGGGCGAACTGGGTCAGCCGGTGCTGGTGCACTGCGAAAACGCGCTGATTTGTGACGAACTGGGc                            <  2:902235/150‑1 (MQ=255)
                                                                                                                     ttCGTTGCGACCTGTGGCGATCGCGGTATAGACAACGACTTCCGTGATGTAAACGACTGGCAGTTCTTCAAAGGTGCGCAGAAGCTGGGCGAACTGGGTCAGCCGGTGCTGGTGCACTGCGAAAACGCGCTGATTTGTGACGAACTGGGc                            <  2:1146098/150‑1 (MQ=255)
                                                                                                                      tCGTTGCGACCTGTGGCGATCGCGGTATAGACAACGACTTCCGTGATGTAAACGACTGGCAGTTCTTCAAAGGTGCGCAGAAGCTGGGCGAACTGGGTCAGCCGGTGCTGGTGCACTGCGAAAACGCGCTGATTTGTGACGAACTGGGCg                           <  1:1062913/150‑1 (MQ=255)
                                                                                                                      tCGTTGCGACCTGTGGCGATCGCGGTATAGACAACGACTTCCGTGATGTAAACGACTGGCAGTTCTTCAAAGGTGCGCAGAAGCTGGGCGAACTGGGTCAGCCGGTGCTGGTGCACTGCGAAAACGCGCTGATTTGTGACGAACTGGGCg                           <  2:2966552/150‑1 (MQ=255)
                                                                                                                       cGTTGCGACCTGTGGCGATCGCGGTATAGACAACGACTTCCGTGATGTAAACGACTGGCAGTTCTTCAAAGGTGCGCAGAAGCTGGGCGAACTGGGTCAGCCGGTGCTGGTGCACTGCGAAAACGCGCTGATTTGTGACGAACTGGGCga                          >  2:1669283/1‑150 (MQ=255)
                                                                                                                        gTTGCGACCTGTGGCGATCGCGGTATAGACAACGACTTCCGTGATGTAAACGACTGGCAGTTCTTCAAAGGTGCGCAGAAGCTGGGCGAACTGGGTCAGCCGGTGCTGGTGCACTGCGAAAACGCGCTGATTTGTGACGAACTGGGCgaa                         <  1:3329668/150‑1 (MQ=255)
                                                                                                                         ttGCGACCTGTGGCGATCGCGGTATAGACAACGACTTCCGTGATGTAAACGACTGGCAGTTCTTCAAAGGTGCGCAGAAGCTGGGCGAACTGGGTCAGCCGGTGCTGGTGCACTGCGAAAACGCGCTGATTTGTGACGAACTGGGCgaag                        <  1:2602939/150‑1 (MQ=255)
                                                                                                                         ttGCGACCTGTGGCGATCGCGGTATAGACAACGACTTCCGTGATGTAAACGACTGGCAGTTCTTCAAAGGTGCGCAGAAGCTGGGCGAACTGGGTCAGCCGGTGCTGGTGCACTGCGAAAACGCGCTGATTTGTGACGAACTGGGCgaag                        <  2:2376500/150‑1 (MQ=255)
                                                                                                                           gCGACCTGTGGCGATCGCGGTATAGACAACGACTTCCGTGATGTAAACGACTGGCAGTTCTTCAAAGGTGCGCAGAAGCTGGGCGAACTGGGTCAGCCGGTGCTGGTGCACTGCGAAAACGCGCTGATTTGTGACGAACTGGGCgaagaa                      <  1:3094436/150‑1 (MQ=255)
                                                                                                                           gCGACCTGTGGCGATCGCGGTATAGACAACGACTTCCGTGATGTAAACGACTGGCAGTTCTTCAAAGGTGCGCAGAAGCTGGGCGAACTGGGTCAGCCGGTGCTGGTGCACTGCGAAAACGCGCTGATTTGTGACGAACTGGGCgaagaa                      <  1:3094823/150‑1 (MQ=255)
                                                                                                                             gACCTGTGGCGATCGCGGTATAGACAACGACTTCCGTGATGTAAACGACTGGCAGTTCTTCAAAGGTGCGCAGAAGCTGGGCGAACTGGGTCAGCCGGTGCTGGTGCACTGCGAAAACGCGCTGATTTGTGACGAACTGGGCGAAgaagc                    <  2:1421048/150‑1 (MQ=255)
                                                                                                                              aCCTGTGGCGATCGCGGTATAGACAACGACTTCCGTGATGTAAACGACTGGCAGTTCTTCAAAGGTGCGCAGAAGCTGGGCGAACTGGGTCAGCCGGTGCTGGTGCACTGCGAAAACGCGCTGATTTGTGACGAACTGGGCGAAgaagcg                   >  1:2432484/1‑150 (MQ=255)
                                                                                                                                cTGTGGCGATCGCGGTATAGACAACGACTTCCGTGATGTAAACGACTGGCAGTTCTTCAAAGGTGCGCAGAAGCTGGGCGAACTGGGTCAGCCGGTGCTGGTGCACTGCGAAAACGCGCTGATTTGTGACGAACTGGGCGAAgaagcgaa                 <  2:733320/150‑1 (MQ=255)
                                                                                                                                 tgtgGCGATCGCGGTATAGACAACGACTTCCGTGATGTAAACGACTGGCAGTTCTTCAAAGGTGCGCAGAAGCTGGGCGAACTGGGTCAGCCGGTGCTGGTGCACTGCGAAAACGCGCTGATTTGTGACGAACTGGGCGAAgaagcgaag                >  1:1033883/1‑150 (MQ=255)
                                                                                                                                  gtgGCGATCGCGGTATAGACAACGACTTCCGTGATGTAAACGACTGGCAGTTCTTCAAAGGTGCGCAGAAGCTGGGCGAACTGGGTCAGCCGGTGCTGGTGCACTGCGAAAACGCGCTGATTTGTGACGAACTGGGCGAAgaagcgaagc               <  2:1587101/150‑1 (MQ=255)
                                                                                                                                    ggCGATCGCGGTATAGACAACGACTTCCGTGATGTAAACGACTGGCAGTTCTTCAAAGGTGCGCAGAAGCTGGGCGAACTGGGTCAGCCGGTGCTGGTGCACTGCGAAAACGCGCTGATTTGTGACGAACTGGGCGAAGAAGCGAAGCGt             <  1:769316/150‑1 (MQ=255)
                                                                                                                                       gATCGCGGTATAGACAACGACTTCCGTGATGTAAACGACTGGCAGTTCTTCAAAGGTGCGCAGAAGCTGGGCGAACTGGGTCAGCCGGTGCTGGTGCACTGCGAAAACGCGCTGATTTGTGACGAACTGGGCGAAGAAGCGAAGCGTGaa          >  1:2615603/1‑150 (MQ=255)
                                                                                                                                       gATCGCGGTATAGACAACGACTTCCGTGATGTAAACGACTGGCAGTTCTTCAAAGGTGCGCAGAAGCTGGGCGAACTGGGTCAGCCGGTGCTGGTGCACTGCGAAAACGCGCTGATTTGTGACGAACTGGGCGAAGAAGCGAAGCGTGaa          >  1:2614598/1‑150 (MQ=255)
                                                                                                                                       gATCGCGGTATAGACAACGACTTCCGTGATGTAAACGACTGGCAGTTCTTCAAAGGTGCGCAGAAGCTGGGCGAACTGGGTCAGCCGGTGCTGGTGCACTGCGAAAACGCGCTGATTTGTGACGAACTGGGCGAAGAAGCGAAGCGTGaa          >  1:968380/1‑150 (MQ=255)
                                                                                                                                       gATCGCGGTATAGACAACGACTTCCGTGATGTAAACGACTGGCAGTTCTTCAAAGGTGCGCAGAAGCTGGGCGAACTGGGTCAGCCGGTGCTGGTGCACTGCGAAAACGCGCTGATTTGTGACGAACTGGGCGAAGAAGCGAAGCGTGaa          <  1:1409439/150‑1 (MQ=255)
                                                                                                                                        aTCGCGGTATAGACAACGACTTCCGTGATGTAAACGACTGGCAGTTCTTCAAAGGTGCGCAGAAGCTGGGCGAACTGGGTCAGCCGGTGCTGGTGCACTGCGAAAACGCGCTGATTTGTGACGAACTGGGCGAAGAAGCGAAGCGTGAAg         <  2:649547/150‑1 (MQ=255)
                                                                                                                                         tCGCGGTATAGACAACGACTTCCGTGATGTAAACGACTGGCAGTTCTTCAAAGGTGCGCAGAAGCTGGGCGAACTGGGTCAGCCGGTGCTGGTGCACTGCGAAAACGCGCTGATTTGTGACGAACTGGGCGAAGAAGCGAAGCGTGAAgg        >  1:3408823/1‑150 (MQ=255)
                                                                                                                                         tCGCGGTATAGACAACGACTTCCGTGATGTAAACGACTGGCAGTTCTTCAAAGGTGCGCAGAAGCTGGGCGAACTGGGTCAGCCGGTGCTGGTGCACTGCGAAAACGCGCTGATTTGTGACGAACTGGGCGAAGAAGCGAAGCGTGAAgg        <  2:796859/150‑1 (MQ=255)
                                                                                                                                            cgGTATAGACAACGACTTCCGTGATGTAAACGACTGGCAGTTCTTCAAAGGTGCGCAGAAGCTGGGCGAACTGGGTCAGCCGGTGCTGGTGCACTGCGAAAACGCGCTGATTTGTGACGAACTGGGCGAAGAAGCGAAGCGTGAAGGTcg     >  1:481091/1‑150 (MQ=255)
                                                                                                                                               tataGACAACGACTTCCGTGATGTAAACGACTGGCAGTTCTTCAAAGGTGCGCAGAAGCTGGGCGAACTGGGTCAGCCGGTGCTGGTGCACTGCGAAAACGCGCTGATTTGTGACGAACTGGGCGAAGAAGCGAAGCGTGAAGGTCGCGt  >  2:2601071/1‑150 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
TTCGATGCCGCTAAAGGCAAGCTGACTATTGATGCGGCACAACTCGGTGGCCTGGTGTCTTACAACATCGACCGTCTGCATGAGCTGGATGAAGTGGGCGTTGTCGGCTTCAAATGCTTCGTTGCGACCTGTGGCGATCGCGGTATCGACAACGACTTCCGTGATGTAAACGACTGGCAGTTCTTCAAAGGTGCGCAGAAGCTGGGCGAACTGGGTCAGCCGGTGCTGGTGCACTGCGAAAACGCGCTGATTTGTGACGAACTGGGCGAAGAAGCGAAGCGTGAAGGTCGCGT  >  CP009273/534927‑535219

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
AAGTTCGATGCCGCTAAAGGCAAGCTGACTATTGATGCGGCACAACTCGGTGGCCTGGTGTCTTACAACATCGACCGTCTGCATGAGCTGGATGAAGTGGGCGTTGTCGGCTTCAAATGCTTCGTTGCGACCTGTGGCGATCGCGGTATCGACAACGACTTCCGTGATGTAAACGACTGGCAGTTCTTCAAAGGTGCGCAGAAGCTGGGCGAACTGGGTCAGCCGGTGCTGGTGCACTGCGAAAACGCGCTGATTTGTGACGAACTGGGCGAAGAAGCGAAGCGTGAAGGTCGCGTAAC  >  CP009273/534924‑535222
                                                                                                                                                     |                                                                                                                                                     
AAGTTCGATGCCGCTAAAGGCAAGCTGACTATTGATGCGGCACAACTCGGTGGCCTGGTGTCTTACAACATCGACCGTCTGCATGAGCTGGATGAAGTGGGCGTTGTCGGCTTCAAATGCTTCGTTGCGACCTGTGGCGATCGCGGTATA                                                                                                                                                       <  SRR19973061.827770/150‑1 (MQ=60)
     CGATGCCGCTAAAGGCAAGCTGACTATTGATGCGGCACAACTCGGTGGCCTGGTGTCTTACAACATCGACCGTCTGCATGAGCTGGATGAAGTGGGCGTTGTCGGCTTCAAATGCTTCGTTGCGACCTGTGGCGATCGCGGTATAGACAA                                                                                                                                                  >  SRR19973061.1994758/1‑150 (MQ=60)
      GATGCCGCTAAAGGCAAGCTGACTATTGATGCGGCACAACTCGGTGGCCTGGTGTCTTACAACATCGACCGTCTGCATGAGCTGGATGAAGTGGGCGTTGTCGGCTTCAAATGCTTCGTTGCGACCTGTGGCGATCGCGGTATAGACAAC                                                                                                                                                 >  SRR19973061.22624/1‑150 (MQ=60)
      GATGCCGCTAAAGGCAAGCTGACTATTGATGCGGCACAACTCGGTGGCCTGGTGTCTTACAACATCGACCGTCTGCATGAGCTGGATGAAGTGGGCGTTGTCGGCTTCAAATGCTTCGTTGCGACCTGTGGCGATCGCGGTATAGACAAC                                                                                                                                                 >  SRR19973061.820481/1‑150 (MQ=60)
        TGCCGCTAAAGGCAAGCTGACTATTGATGCGGCACAACTCGGTGGCCTGGTGTCTTACAACATCGACCGTCTGCATGAGCTGGATGAAGTGGGCGTTGTCGGCTTCAAATGCTTCGTTGCGACCTGTGGCGATCGCGGTATAGACAACGA                                                                                                                                               >  SRR19973061.1879881/1‑150 (MQ=60)
          CCGCTAAAGGCAAGCTGACTATTGATGCGGCACAACTCGGTGGCCTGGTGTCTTACAACATCGACCGTCTGCATGAGCTGGATGAAGTGGGCGTTGTCGGCTTCAAATGCTTCGTTGCGACCTGTGGCGATCGCGGTATAGACAACGACA                                                                                                                                             <  SRR19973061.3510405/150‑1 (MQ=60)
             CTAAAGGCAAGCTGACTATTGATGCGGCACAACTCGGTGGCCTGGTGTCTTACAACATCGACCGTCTGCATGAGCTGGATGAAGTGGGCGTTGTCGGCTTCAAATGCTTCGTTGCGACCTGTGGCGATCGCGGTATAGACAACGACTTCC                                                                                                                                          <  SRR19973061.544125/150‑1 (MQ=60)
             CTAAAGGCAAGCTGACTATTGATGCGGCACAACTCGGTGGCCTGGTGTCTTACAACATCGACCGTCTGCATGAGCTGGATGAAGTGGGCGTTGTCGGCTTCAAATGCTTCGTTGCGACCTGTGGCGATCGCGGTATAGACAACGACTTCC                                                                                                                                          >  SRR19973061.529363/1‑150 (MQ=60)
              TAAAGGCAAGCTGACTATTGATGCGGCACAACTCGGTGGCCTGGTGTCTTACAACATCGACCGTCTGCATGAGCTGGATGAAGTGGGCGTTGTCGGCTTCAAATGCTTCGTTGCGACCTGTGGCGATCGCGGTATAGACAACGACTTCCG                                                                                                                                         <  SRR19973061.1087293/150‑1 (MQ=60)
                AAGGCAAGCTGACTATTGATGCGGCACAACTCGGTGGCCTGGTGTCTTACAACATCGACCGTCTGCATGAGCTGGATGAAGTGGGCGTTGTCGGCTTCAAATGCTTCGTTGCGACCTGTGGCGATCGCGGTATAGACAACGACTTCCGTG                                                                                                                                       >  SRR19973061.3753839/1‑150 (MQ=60)
                AAGGCAAGCTGACTATTGATGCGGCACAACTCGGTGGCCTGGTGTCTTACAACATCGACCGTCTGCATGAGCTGGATGAAGTGGGCGTTGTCGGCTTCAAATGCTTCGTTGCGACCTGTGGCGATCGCGGTATAGACAACGACTTCCGTG                                                                                                                                       >  SRR19973061.275482/1‑150 (MQ=60)
                      AGCTGACTATTGATGCGGCACAACTCGGTGGCCTGGTGTCTTACAACATCGACCGTCTGCATGAGCTGGATGAAGTGGGCGTTGTCGGCTTCAAATGCTTCGTTGCGACCTGTGGCGATCGCGGTATAGACAACGACTTCCGTGATGTAA                                                                                                                                 <  SRR19973061.1335763/150‑1 (MQ=60)
                       GCTGACTATTGATGCGGCACAACTCGGTGGCCTGGTGTCTTACAACATCGACCGTCTGCATGAGCTGGATGAAGTGGGCGTTGTCGGCTTCAAATGCTTCGTTGCGACCTGTGGCGATCGCGGTATAGACAACGACTTCCGTGATGTAAA                                                                                                                                >  SRR19973061.798948/1‑150 (MQ=60)
                        CTGACTATTGATGCGGCACAACTCGGTGGCCTGGTGTCTTACAACATCGACCGTCTGCATGAGCTGGATGAAGTGGGCGTTGTCGGCTTCAAATGCTTCGTTGCGACCTGTGGCGATCGCGGTATAGACAACGACTTCCGTGATGTAAAC                                                                                                                               <  SRR19973061.3675685/150‑1 (MQ=60)
                        CTGACTATTGATGCGGCACAACTCGGTGGCCTGGTGTCTTACAACATCGACCGTCTGCATGAGCTGGATGAAGTGGGCGTTGTCGGCTTCAAATGCTTCGTTGCGACCTGTGGCGATCGCGGTATAGACAACGACTTCCGTGATGTAAAC                                                                                                                               <  SRR19973061.3578732/150‑1 (MQ=60)
                        CTGACTATTGATGCGGCACAACTCGGTGGCCTGGTGTCTTACAACATCGACCGTCTGCATGAGCTGGATGAAGTGGGCGTTGTCGGCTTCAAATGCTTCGTTGCGACCTGTGGCGATCGCGGTATAGACAACGACTTCCGTGATGTAAAC                                                                                                                               <  SRR19973061.187253/150‑1 (MQ=60)
                        CTGACTATTGATGCGGCACAACTCGGTGGCCTGGTGTCTTACAACATCGACCGTCTGCATGAGCTGGATGAAGTGGGCGTTGTCGGCTTCAAATGCTTCGTTGCGACCTGTGGCGATCGCGGTATAGACAACGACTTCCGTGATGTAAAC                                                                                                                               <  SRR19973061.1680129/150‑1 (MQ=60)
                              ATTGATGCGGCACAACTCGGTGGCCTGGTGTCTTACAACATCGACCGTCTGCATGAGCTGGATGAAGTGGGCGTTGTCGGCTTCAAATGCTTCGTTGCGACCTGTGGCGATCGCGGTATAGACAACGACTTCCGTGATGTAAACGACTGG                                                                                                                         >  SRR19973061.1022516/1‑150 (MQ=60)
                                         ACAACTCGGTGGCCTGGTGTCTTACAACATCGACCGTCTGCATGAGCTGGATGAAGTGGGCGTTGTCGGCTTCAAATGCTTCGTTGCGACCTGTGGCGATCGCGGTATAGACAACGACTTCCGTGATGTAAACGACTGGCAGTTCTTCAA                                                                                                              >  SRR19973061.1732612/1‑150 (MQ=60)
                                         ACAACTCGGTGGCCTGGTGTCTTACAACATCGACCGTCTGCATGAGCTGGATGAAGTGGGCGTTGTCGGCTTCAAATGCTTCGTTGCGACCTGTGGCGATCGCGGTATAGACAACGACTTCCGTGATGTAAACGACCGGCAGTTCTTCAA                                                                                                              >  SRR19973061.3632214/1‑150 (MQ=60)
                                          CAACTCGGTGGCCTGGTGTTTTACAACATCGACCGTCTGCATGAGCTGGATGATGTGGGCGTTGTCGGCTTCAAATGCTTCGTTGCGACCTGTGGCGATCGCGGTATAGACAACGACTTCCGTGATGTAAACGACTGGCAGTTCTTCAAA                                                                                                             <  SRR19973061.2926247/150‑1 (MQ=60)
                                            ACTCGGTGGCATGGTGTTTTACAACATCGACCGTTGGCATGAGCTGGATGATGTGGGTGTTGTCGGCTTAAAATGATTCGTTGCGACCTGTGGCGATCGCGGTATAGACAACGACTTCCGTGATTTAAACGACTGGCAGTTCTTCAAAGG                                                                                                           <  SRR19973061.2343988/150‑1 (MQ=60)
                                              TCGGTGGCCTGGTGTCTTACAACATCGACCGTCTGCATGAGCTGGATGAAGTGGGCGTTGTCGGCTTCAAATGCTTCGTTGCGACCTGTGGCGATCGCGGTATAGACAACGACTTCCGTGATGTAAACGACTGGCAGTTCTTCAAAGGTG                                                                                                         >  SRR19973061.3447035/1‑150 (MQ=60)
                                              TCGGTGGCCTGGTGTCTTACAACATCGACCGTCTGCATGAGCTGGATGAAGTGGGCGTTGTCGGCTTCAAATGCTTCGTTGCGACCTGTGGCGATCGCGGTATAGACAACGACTTCCGTGATGTAAACGACTGGCAGTTCTTCAAAGGTG                                                                                                         >  SRR19973061.1546827/1‑150 (MQ=60)
                                                  TGGCCTGGTGTCTTACAACATCGACCGTCTGCATGAGCTGGATGAAGTGGGCGTTGTCGGCTTCAAATGCTTCGTTGCGACCTGTGGCGATCGCGGTATAGACAACGACTTCCGTGATGTAAACGACTGGCAGTTCTTCAAAGGTGCGCA                                                                                                     <  SRR19973061.3409939/150‑1 (MQ=60)
                                                    GCCTGGTGTCTTACAACATCGACCGTCTGCATGAGCTGGATGAAGTGGGCGTTGTCGGCTTCAAATGCTTCGTTGCGACCTGTGGCGATCGCGGTATAGACAACGACTTCCGTGATGTAAACGACTGGCAGTTCTTCAAAGGTGCGCAGA                                                                                                   >  SRR19973061.3248058/1‑150 (MQ=60)
                                                    GCCTGGTGTCTTACAACATCGACCGTCTGCATGAGCTGGATGAAGTGGGCGTTGTCGGCTTCAAATGCTTCGTTGCGACCTGTGGCGATCGCGGTATAGACAACGACTTCCGTGATGTAAACGACTGGCAGTTCTTCAAAGGTGCGCAGA                                                                                                   >  SRR19973061.2033713/1‑150 (MQ=60)
                                                       TGGTGTCTTACAACATCGACCGTCTGCATGAGCTGGATGAAGTGGGCGTTGTCGGCTTCAAATGCTTCGTTGCGACCTGTGGCGATCGCGGTATAGACAACGACTTCCGTGATGTAAACGACTGGCAGTTCTTCAAAGGTGCGCAGAAGC                                                                                                >  SRR19973061.1632850/1‑150 (MQ=60)
                                                        GGTGTCTTACAACATCGACCGTCTGCATGAGCTGGATGATGTGGGCGTTGTCGGCTTCAAATGCTTCGTTGCGACCTGTGGCGATCGCGGTATAGACAACGACTTCCGTGATGTAAACGACTGGCAGTTCTTCAAAGGTGCGCAGAAGCT                                                                                               <  SRR19973061.3297223/150‑1 (MQ=60)
                                                         GTGTCTTACAACATCGACCGTCTGCATGAGCTGGATGAAGTGGGCGTTGTCGGCTTCAAATGCTTCGTTGCGACCTGTGGCGATCGCGGTATAGACAACGACTTCCGTGATGTAAACGACTGGCAGTTCTTCAAAGGTGCGCAGAAGCTG                                                                                              >  SRR19973061.2008573/1‑150 (MQ=60)
                                                         GTGTCTTACAACATCGACCGTCTGCATGAGCTGGATGAAGTGGGCGTTGTCGGCTTCAAATGCTTCGTTGCGACCTGTGGCGATCGCGGTATAGACAACGACTTCCGTGATGTAAACGACTGGCAGTTCTTCAAAGGTGCGCAGAAGCTG                                                                                              >  SRR19973061.92871/1‑150 (MQ=60)
                                                         GTGTCTTACAACATCGACCGTCTGCATGAGCTGGATGAAGTGGGCGTTGTCGGCTTAAAATGCTTCGTTGCGACCTGTGGCGATCGCGGTATAGACAACGACTTCCGTGATGTAAACGACTGGCAGTTCTTCAAAGGTGCGCAGAAGCTG                                                                                              <  SRR19973061.1071582/150‑1 (MQ=60)
                                                                   ACATCGACCGTCTGCATGAGCTGGATGAAGTGGGCGTTGTCGGCTTCAAATGCTTCGTTGCGACCTGTGGCGATCGCGGTATAGACAACGACTTCCGTGATGTAAACGACTGGCAGTTCTTCAAAGGTGCGCAGAAGCTGGGCGAACTGG                                                                                    <  SRR19973061.2066946/150‑1 (MQ=60)
                                                                    CATCGACCGTCTGCATGAGCTGGATGAAGTGGGCGTTGTCGGCTTCAAATGCTTCGTTGCGACCTGTGGCGATCGCGGTATAGACAACGACTTCCGTGATGTAAACGACTGGCAGTTCTTCAAAGGTGCGCAGAAGCTGGGCGAACTGGG                                                                                   >  SRR19973061.1870728/1‑150 (MQ=60)
                                                                     ATCGACCGTCTGCATGAGCTGGATGAAGTGGGCGTTGTCGGCTTCAAATGCTTCGTTGCGACCTGTGGCGATCGCGGTATAGACAACGACTTCCGTGATGTAAACGACTGGCAGTTCTTCAAAGGTGCGCAGAAGCTGGGCGAACTGGGT                                                                                  >  SRR19973061.651212/1‑150 (MQ=60)
                                                                     ATCGACCGTCTGCATGAGCTGGATGAAGTGGGCGTTGTCGGCTTCAAATGCTTCGTTGCGACCTGTGGCGATCGCGGTATAGACAACGACTTCCGTGATGTAAACGACTGGCAGTTCTTCAAAGGTGCGCAGAAGCTGGGCGAACTGGGT                                                                                  >  SRR19973061.651190/1‑150 (MQ=60)
                                                                        GACCGTCTGCATGAGCTGGATGAAGTGGGCGTTGTCGGCTTCAAATGCTTCGTTGCGACCTGTGGCGATCGCGGTATAGACAACGACTTCCGTGATGTAAACGACTGGCAGTTCTTCAAAGGTGCGCAGAAGCTGGGCGAACTGGGTCAG                                                                               <  SRR19973061.1173063/150‑1 (MQ=60)
                                                                               TGCATGAGCTGGATGAAGTGGGCGTTGTCGGCTTCAAATGCTTCGTTGCGACCTGTGGCGATCGCGGTATAGACAACGACTTCCGTGATGTAAACGACTGGCAGTTCTTCAAAGGTGCGCAGAAGCTGGGCGAACTGGGTCAGCCGGTGC                                                                        <  SRR19973061.2294109/150‑1 (MQ=60)
                                                                               TGCATGAGCTGGATGAAGTGGGCGTTGTCGGATTCAAATGCTTCGTTGCGACCTGTGGCGATCGCGGTATAGACAACGACTTCCGTGATGTAAACGACTGGCAGTTCTTCAAAGGTGCGCAGAAGCTGGGCGAACTGGGTCAGCCGGTGC                                                                        <  SRR19973061.383109/150‑1 (MQ=60)
                                                                                 CATGTGCTGGATGATGTGGGCGTTGTCGGCTTCAAATGCTTCGTTGCGACCTGTGGCGATCGCGGTATAGACAACGACTTCCGTGATGTAAACGACTGGCAGTTCTTCAAAGGTGCGCAGAAGCTGGGCGAACTGGGTCAGCCGGTGCTG                                                                      <  SRR19973061.679430/150‑1 (MQ=60)
                                                                                 CATGAGCTGGATGAAGTGGGCGTTGTCGGCTTCAAATGCTTCGTTGCGACCTGTGGCGATCGCGGTATAGACAACGACTTCCGTGATGTAAACGACTGGCAGTTCTTCAAAGGTGCGCAGAAGCTGGGCGAACTGGGTCAGCCGGTGCTG                                                                      >  SRR19973061.3190669/1‑150 (MQ=60)
                                                                                  ATGAGCTGGATGAAGTGGGCGTTGTCGGCTTCAAATGCTTCGTTGCGACCTGTGGCGATCGCGGTATAGACAACGACTTCCGTGATGTAAACGACTGGCAGTTCTTCAAAGGTGCGCAGAAGCTGGGCGAACTGGGTCAGCCGGTGCTGG                                                                     >  SRR19973061.998947/1‑150 (MQ=60)
                                                                                    GAGCTGGATGAAGTGGGCGTTGTCGGCTTCAAATGCTTCGTTGCGACCTGTGGCGATCGCGGTATAGACAACGACTTCCGTGATGTAAACGACTGGCAGTTCTTCAAAGGTGCGCAGAAGCTGGGCGAACTGGGTCAGCCGGTGCTGGTG                                                                   >  SRR19973061.2387289/1‑150 (MQ=60)
                                                                                    GAGCTGGATGAAGTGGGCGTTGTCGGCTTCAAATGCTTCGTTGCGACCTGTGGCGATCGCGGTATAGACAACGACTTCCGTGATGTAAACGACTGGCAGTTCTTCAAAGGTGCGCAGAAGCTGGGCGAACTGGGTCAGCCGGTGCTGGTG                                                                   <  SRR19973061.1714592/150‑1 (MQ=60)
                                                                                           ATGAAGTGGGCGTTGTCGGCTTCAAATGCTTCGTTGCGACCTGTGGCGATCGCGGTATAGACAACGACTTCCGTGATGTAAACGACTGGCAGTTCTTCAAAGGTGCGCAGAAGCTGGGCGAACTGGGTCAGCCGGTGCTGGTGCACTGCG                                                            >  SRR19973061.3141877/1‑150 (MQ=60)
                                                                                           ATGAAGTGGGCGTTGTCGGCTTCAAATGCTTCGTTGCGACCTGTGGCGATCGCGGTATAGACAACGACTTCCGTGATGTAAACGACTGGCAGTTCTTCAAAGGTGCGCAGAAGCTGGGCGAACTGGGTCAGCCGGTGCTGGTGCACTGCG                                                            >  SRR19973061.1429054/1‑150 (MQ=60)
                                                                                                GTGGGCGTTGTCGGCTTCAAATGCTTCGTTGCGACCTGTGGCGATCGCGGTATAGACAACGACTTCCGTGATGTAAACGACTGGCAGTTCTTCAAAGGTGCGCAGAAGCTGGGCGAACTGGGTCAGCCGGTGCTGGTGCACTGCGAAAAC                                                       <  SRR19973061.1063905/150‑1 (MQ=60)
                                                                                                GTGGGCGTTGTCGGCTTCAAATGCTTCGTTGCGACCTGTGGCGATCGCGGTATAGACAACGACTTCCGTGATGTAAACGACTGGCAGTTCTTCAAAGGTGCGCAGAAGCTGGGCGAACTGGGTCAGCCGGTGCTGGTGCACTGCGAAAAC                                                       <  SRR19973061.3259429/150‑1 (MQ=60)
                                                                                                GTGGGCGGTGTCGGCTTCAAATGCTTCGTTGCGACCTGTGGCGATCGCGGTATAGACAACGACTTCCGTGATGTAAACGACTGGCAGTTCTTCAAAGGTGCGCAGAAGCTGGGCGAACTGGGTCAGCCGGTGCTGGTGCACTGCGAAAAC                                                       <  SRR19973061.1064069/150‑1 (MQ=60)
                                                                                                 TNGGCGTTGTCGGCTTCAAATGCTTCGTTGCGACCTGTGGCGATCGCGGTATAGACAACGACTTCCGTGATGTAAACGACTGGCAGTTCTTCAAAGGTGCGCAGAAGCTGGGCGAACTGGGTCAGCCGGTGCTGGTGCACTGCGAAAACG                                                      >  SRR19973061.49161/1‑150 (MQ=60)
                                                                                                 TGGGCGTTGTCGGCTTCAAATGCTTCGTTGCGACCTGTGGCGATCGCGGTATAGACAACGACTTCCGTGATGTAAACGACTGGCAGTTCTTCAAAGGTGCGCAGAAGCTGGGCGAACTGGGTCAGCCGGTGCTGGTGCACTGCGAAAACG                                                      <  SRR19973061.3437294/150‑1 (MQ=60)
                                                                                                 TGGGCGTTGTCGGCTTCAAATGCTTCGTTGCGACCTGTGGCGATCGCGGTATAGACAACGACTTCCGTGATGTAAACGACTGGCAGTTCTTCAAAGGTGCGCAGAAGCTGGGCGAACTGGGTCAGCCGGTGCTGGTGCACTGCGAAAACG                                                      <  SRR19973061.3437443/150‑1 (MQ=60)
                                                                                                  GGGCGTTGTCGGCTTCAAATGCTTCGTTGCGACCTGTGGCGATCGCGGTATAGACAACGATTTCCGTGATGTAAACGACTGGCAGTTCTTCAAAGGTGCGCAGAAGCTGGGCGAACTGGGTCAGCCGGTGCTGGTGCACTGCGAAAACGC                                                     <  SRR19973061.1426469/150‑1 (MQ=60)
                                                                                                  GGGCGTTGTCGGCTTCAAATGCTTCGTTGCGACCTGTGGCGATCGCGGTATAGACAACGACTTCCGTGATGTAAACGACTGGCAGTTCTTCAAAGGTGCGCAGAAGCTGGGCGAACTGGGTCAGCCGGTGCTGGTGCACTGCGAAAACGC                                                     <  SRR19973061.1426224/150‑1 (MQ=60)
                                                                                                     CGTTGTCGGCTTCAAATGCTTCGTTGCGACCTGTGGCGATCGCGGTATAGACAACGACTTCCGTGATGTAAACGACTGGCAGTTCTTCAAAGGTGCGCAGAAGCTGGGCGAACTGGGTCAGCCGGTGCTGGTGCACTGCGAAAACGCGCT                                                  >  SRR19973061.2449882/1‑150 (MQ=60)
                                                                                                      gtggtgggcttcataggtTTCGTTGCGACCTGTGGTGATCGCGGTATAGACAACGACTTCCGTGATGTAAACGACTGGCAGTTCTTCAAAGGTGCGCAGAAGCTGGGCGAACTGGGTCAGCCGGTGCTGGTGCACTGCGAAAACGCGCTG                                                 <  SRR19973061.648292/132‑1 (MQ=60)
                                                                                                      GTTGTCGGCTTCAAATGCTTCGTTGCGACCTGTGGCGATCGCGGTATAGACAACGACTTCCGTGATGTAAACGACTGGCAGTTCTTCAAAGGTGCGCAGAAGCTGGGCGAACTGGGTCAGCCGGTGCTGGTGCACTGCGAAAACGCGCTG                                                 >  SRR19973061.2562008/1‑150 (MQ=60)
                                                                                                      GTTGTCGGCTTCAAATGCTTCGTTGCGACCTGTGGCGATCGCGGTATAGACAACGACTTCCGTGATGTAAACGACTGGCAGTTCTTCAAAGGTGCGCAGAAGCTGGGCGAACTGGGTCAGCCGGTGCTGGTGCACTGCGAAAACGCGCTG                                                 <  SRR19973061.2702523/150‑1 (MQ=60)
                                                                                                      GTTGTCGGCTTCAAATGCTTCGTTGCGACCTGTGGCGATCGCGGTATAGACAACGACTTCCGTGATGTAAACGACTGGCAGTTCTTCAAAGGTGCGCAGAAGCTGGGCGAACTGGGTCAGCCGGTGCTGGTGCACTGCGAAAACGCGCTG                                                 <  SRR19973061.792591/150‑1 (MQ=60)
                                                                                                      GTTGTCGGCTTCAAATGCTTCGTTGCGACCTGTGGCGATCGCGGTATAGACAACGACTTCCGTGATGTAAACGACTGGCAGTTCTTCAAAGGTGCGCAGAAGCTGGGCGAACTGGGTCAGCCGGTGCTGGTGCACTGCGAAAACGCGCTG                                                 >  SRR19973061.1589942/1‑150 (MQ=60)
                                                                                                         GTCGGCTTCAAATGCTTCGTTGCGACCTGTGGCGATCGCGGTATAGACAACGACTTCCGTGATGTAAACGACTGGCAGTTCTTCAAAGGTGCGCAGAAGCTGGGCGAACTGGGTCAGCCGGTGCTGGTGCACTGCGAAAACGCGCTGATT                                              >  SRR19973061.3386901/1‑150 (MQ=60)
                                                                                                          TCGGCTTCAATTGCTTCGTTGCGACCTGTGGCGATCGCGGTATAGACAACGACTTCCGTGATGTAAACGACTGGCAGTTCTTCAAAGGTGCGCAGAAGCTGGGCGAACTGGGTCAGCCGGTGCTGGTGCACTGCGAAAACGCGCTGATTT                                             <  SRR19973061.663725/150‑1 (MQ=60)
                                                                                                          TCGGCTTCAAATGCTTCGTTGCGACCTGTGGCGATCGCGGTATAGACAACGACTTCCGTGATGTAAACGACTGGCAGTTCTTCAAAGGTGCGCAGAAGCTGGGCGAACTGGGTCAGCCGGTGCTGGTGCACTGCGAAAACGCGCTGATTT                                             <  SRR19973061.2575405/150‑1 (MQ=60)
                                                                                                            GGCTTCAAATGCTTCGTTGCGACCTGTGGCGATCGCGGTATAGACAACGACTTCCGTGATGTAAACGACTGGCAGTTCTTCAAAGGTGCGCAGAAGCTGGGCGAACTGGGTCAGCCGGTGCTGGTGCACTGCGAAAACGCGCTGATTTGT                                           <  SRR19973061.2353862/150‑1 (MQ=60)
                                                                                                             GCTTCAAATGCTTCGTTGCGACCTGTGGCGATCGCGGTATAGACAACGACTTCCGTGATGTAAACGACTGGCAGTTCTTCAAAGGTGCGCAGAAGCTGGGCGAACTGGGTCAGCCGGTGCTGGTGCACTGCGAAAACGCGCTGATTTGTG                                          <  SRR19973061.3286477/150‑1 (MQ=60)
                                                                                                               TTCAAATGTTTCGTTGCGACCTGTGGCGATCGCGGTATAGACAACGACTTCCGTGATGTAAACGACTGGCAGTTCTTCAAAGGTGCGCAGAAGCTGGGCGAACTGGGTCAGCCGGTGCTGGTGCACTGCGAAAACGCGCTGATTTGTGAC                                        <  SRR19973061.1315570/150‑1 (MQ=60)
                                                                                                               TTCAAATGCTTCGTTGCGACCTGTGGCGATCGCGGTATAGACAACGACTTCCGTGATGTAAACGACTGGCAGTTCTTCAAAGGTGCGCAGAAGCTGGGCGAACTGGGTCAGCCGGTGCTGGTGCACTGCGAAAACGCGCTGATTTGTGAC                                        >  SRR19973061.252435/1‑150 (MQ=60)
                                                                                                                 CAAATGCTTCGTTGCGACCTGTGGCGATCGCGGTATAGACAACGACTTCCGTGATGTAAACGACTGGCAGTTCTTCAAAGGTGCGCAGAAGCTGGGCGAACTGGGTCAGCCGGTGCTGGTGCACTGCGAAAACGCGCTGATTTGTGACGA                                      >  SRR19973061.3428772/1‑150 (MQ=60)
                                                                                                                  AAATGCTTCGTTGCGACCTGTGGCGATCGCGGTATAGACAACGACTTCCGTGATGTAAACGACTGGCAGTTCTTCAAAGGTGTGCAGAAGCTGGGCGAACTGGGTCAGCCGGTGCTGGTGCACTGCGAAAACGCGCTGAGTTGTGACGAA                                     >  SRR19973061.2999488/1‑150 (MQ=60)
                                                                                                                        TTCGTTGCGTCCTGTGGGGATCGCGGTATATACAACGATTTCGGTTATGTAAACGACTGGCAGTTCTTCAAAGGTGCGCAGAAGCTGGGCGAACTGGGTCAGCCGGTGCTGGTGCACTGCGAAAACGCGCTGATTTGTGACGAACTGGGC                               <  SRR19973061.1028476/150‑1 (MQ=60)
                                                                                                                        TTCGTTGCGACCTGTGGCGATCGCGGTATAGACAACGACTTCCGTGATGTAAACGACTGGCAGTTCTTCAAAGGTGCGCAGAAGCTGGGCGAACTGGGTCAGCCGGTGCTGGTGCACTGCGAAAACGCGCTGATTTGTGACGAACTGGGC                               <  SRR19973061.1028578/150‑1 (MQ=60)
                                                                                                                         TCGTTGCGACCTGTGGCGATCGCGGTATAGACAACGACTTCCGTGATGTAAACGACTGGCAGTTCTTCAAAGGTGCGCAGAAGCTGGGCGAACTGGGTCAGCCGGTGCTGGTGCACTGCGAAAACGCGCTGATTTGTGACGAACTGGGCG                              <  SRR19973061.1065535/150‑1 (MQ=60)
                                                                                                                         TCGTTGCGACCTGTGGCGATCGCGGTATAGACAACGACTTCCGTGATGTAAACGACTGGCAGTTCTTCAAAGGTGCGCAGAAGCTGGGCGAACTGGGTCAGCCGGTGCTGGTGCACTGCGAAAACGCGCTGATTTGTGACGAACTGGGCG                              <  SRR19973061.1065644/150‑1 (MQ=60)
                                                                                                                           GTTGCGACCTGTGGCGATCGCGGTATAGACAACGACTTCCGTGATGTAAACGACTGGCAGTTCTTCAAAGGTGCGCAGAAGCTGGGCGAACTGGGTCAGCCGGTGCTGGTGCACTGCGAAAACGCGCTGATTTGTGACGAACTGGGCGAA                            <  SRR19973061.3338246/150‑1 (MQ=60)
                                                                                                                            TTGCGACCTGTGGCGATCGCGGTATAGACAACGACTTCCGTGATGTAAACGACTGGCAGTTCTTCAAAGGTGCGCAGAAGCTGGGCGAACTGGGTCAGCCGGTGCTGGTGCACTGCGAAAACGCGCTGATTTGTGACGAACTGGGCGAAG                           <  SRR19973061.2609625/150‑1 (MQ=60)
                                                                                                                            TTGCGACCGGTGGCGATCGCGGTATAGACAACGACTTCCGTGATGTAAACGACTGGCAGTTCTTCAAAGGTGCGCAGAAGCTGGGCGAACTGGGTCAGCCGGTGCTGGTGCACTGCGAAAACGCGCTGATTTGTGACGAACTGGGCGAAG                           <  SRR19973061.2609108/150‑1 (MQ=60)
                                                                                                                              GCGACTTGTGGCGATCGCGGTATAGACAACGAATTCTGTGATGTAAACGACTGGCAGTTCTTCAAAGGTGCGCAGAAGCTGGGCGAACTGGGTCAGCCGGTGCTGGTGCACTGCGAAAACGCGCTGATTTGTGACGAACTGGGCGAAGAA                         <  SRR19973061.3102410/150‑1 (MQ=60)
                                                                                                                              GCGACCTGTGGCGATCGCGGTATAGACAACGACTTCCGTGATGTAAACGACTGGCAGTTCTTCAAAGGTGCGCAGAAGCTGGGCGAACTGGGTCAGCCGGTGCTGGTGCACTGCGAAAACGCGCTGATTTGTGACGAACTGGGCGAAGAA                         <  SRR19973061.3102409/150‑1 (MQ=60)
                                                                                                                              GCGACCTGTGGCGATCGCGGTATAGACAACGACTTCCGTGATGTAAACGACTGGCAGTTCTTCAAAGGTGCGCAGAAGCTGGGCGAACTGGGTCAGCCGGTGCTGGTGCACTGCGAAAACGCGCTGATTTGTGACGAACTGGGCGAAGAA                         <  SRR19973061.33366/150‑1 (MQ=60)
                                                                                                                              GCGACCTGTGGCGATCGCGGTATAGACAACGACTTCCGTGATGTAAACGACTGGCAGTTCTTCAAAGGTGCGCAGAAGCTGGGCGAACTGGGTCAGCCGGTGCTGGTGCACTGCGAAAACGCGCTGATTTGTGACGAACTGGGCGAAGAA                         <  SRR19973061.3102798/150‑1 (MQ=60)
                                                                                                                                GACCTGTGGCGATCGCGGTATAGACAACGACTTCCGTGATGTAAACGACTGGCAGTTCTTCAAAGGTGCGCAGAAGCTGGGCGAACTGGGTCAGCCGGTGCTGGTGCACTGCGAAAACGCGCTGATTTGTGACGAACTGGGCGAAGAAGC                       >  SRR19973061.3218154/1‑150 (MQ=60)
                                                                                                                                 ACCTGTGGCGATCGCGGTATAGACAACGACTTCCGTGATGTAAACGACTGGCAGTTCTTCAAAGGTGCGCAGAAGCTGGGCGAACTGGGTCAGCCGGTGCTGGTGCACTGCGAAAACGCGCTGATTTGTGACGAACTGGGCGAAGAAGCG                      >  SRR19973061.2438690/1‑150 (MQ=60)
                                                                                                                                    TGTGGCGATCGCGGTATAGACAACGACTTCCGTGATGTAAACGACTGGCAGTTCTTCAAAGGTGCGCAGAAGCTGGGCGAACTGGGTCAGCCGGTGCTGGTGCACTGCGAAAACGCGCTGATTTGTGACGAACTGGGCGAAGAAGCGAAG                   <  SRR19973061.2874975/150‑1 (MQ=60)
                                                                                                                                    TGTGGCGATCGCGGTATAGACAACGACTTCCGTGATGTAAACGACTGGCAGTTCTTCAAAGGTGCGCAGAAGCTGGGCGAACTGGGTCAGCCGGTGCTGGTGCACTGCGAAAACGCGCTGATTTGTGACGAACTGGGCGAAGAAGCGAAG                   >  SRR19973061.1036533/1‑150 (MQ=60)
                                                                                                                                       GGCGATCGCGGTATAGACAACGACTTCCGTGATGTAAACGACTGGCAGTTCTTCAAAGGTGCGCAGAAGCTGGGCGAACTGGGTCAGCCGGTGCTGGTGCACTGCGAAAACGCGCTGATTTGTGACGAACTGGGCGAAGAAGCGAAGCGT                <  SRR19973061.771335/150‑1 (MQ=60)
                                                                                                                                       GGCGATCGCGGTATAGACAACGACTTCCGTGATGTAAACGACTGGCAGTTCTTCAAAGGTGCGCAGAAGCTGGGCGAACTGGGTCAGCCGGTGCTGGTGCACTGCGAAAACGCGCTGATTTGTGACGAACTGGGCGAAGAAGCGAAGCGT                <  SRR19973061.771063/150‑1 (MQ=60)
                                                                                                                                        GCGATCGCGGTATAGACAACGACTTCCGTGATGTAAACGACTGGCAGTTCTTCAAAGGTGCGCAGAAGCTGGGCGAACTGGGTCAGCCGGTGCTGGTGCACTGCGAAAACGCGCTGATTTGTGACGAACTGGGCGAAGAAGCGAAGCGTG               <  SRR19973061.2430524/150‑1 (MQ=60)
                                                                                                                                          GATCGCGGTATAGACAACGACTTCCGTGATGTAAACGACTGGCAGTTCTTCAAAGGTGCGCAGAAGCTGGGCGAACTGGGTCAGCCGGTGCTGGTGCACTGCGAAAACGCGCTGATTTGTGACGAACTGGGCGAAGAAGCGAAGCGTGNA             <  SRR19973061.1154809/150‑1 (MQ=60)
                                                                                                                                          GATCGCGGTATAGACAACGACTTCCGTGATGTAAACGACTGGCAGTTCTTCAAAGGTGCGCAGAAGCTGGGCGAACTGGGTCAGCCGGTGCTGGTGCACTGCGAAAACGCGCTGATTTGTGACGAACTGGGCGAAGAAGCGAAGCGTGAA             <  SRR19973061.1412996/150‑1 (MQ=60)
                                                                                                                                          GATCGCGGTATAGACAACGACTTCCGTGATGTAAACGACTGGCAGTTCTTCAAAGGTGCGCAGAAGCTGGGCGAACTGGGTCAGCCGGTGCTGGTGCACTGCGAAAACGCGCTGATTTGTGACGAACTGGGCGAAGAAGCGAAGCGTGAA             >  SRR19973061.970830/1‑150 (MQ=60)
                                                                                                                                          GATCGCGGTATAGACAACGACTTCCGTGATGTAAACGACTGGCAGTTCTTCAAAGGTGCGCAGAAGCTGGGCGAACTGGGTCAGCCGGTGCTGGTGCACTGCGAAAACGCGCTGATTTGTGACGAACTGGGCGAAGAAGCGAAGCGTGAA             >  SRR19973061.2621310/1‑150 (MQ=60)
                                                                                                                                          GATCGCGGTATAGACAACGACTTCCGTGATGTAAACGACTGGCAGTTCTTCAAAGGTGCGCAGAAGCTGGGCGAACTGGGTCAGCCGGTGCTGGTGCACTGCGAAAACGCGCTGATTTGTGACGAACTGGGCGAAGAAGCGAAGCGTGAA             >  SRR19973061.2622320/1‑150 (MQ=60)
                                                                                                                                          GATCGCGGTATAGACAACGACTTCCGTGATGTAAACGACTGGCAGTTCTTCAAAGGTGCGCAGAAGCTGGGCGAACTGGGTCAGCCGGTGCTGATGCACTGCGAAAACGCGCTGATTTGTGACGAACTGGGCGAAGAAGCGAAGCGTGAA             >  SRR19973061.2621309/1‑150 (MQ=60)
                                                                                                                                            TCGCGGTATAGACAACGACTTCCGTGATGTAAACGACTGGCAGTTCTTCAAAGGTGCGCAGAAGCTGGGCGAACTGGGTCAGCCGGTGCTGGTGCACTGCGAAAACGCGCTGATTTGTGACGAACTGGGCGAAGAAGCGAAGCGTGAAGG           >  SRR19973061.1514626/1‑150 (MQ=60)
                                                                                                                                            TCGCGGTATAGACAACGACTTCCGTGATGTAAACGACTGGCAGTTCTTCAAAGGTGCGCAGAAGCTGGGCGAACTGGGTCAGCCGGTGCTGGTGCACTGCGAAAACGCGCTGATTTGTGACGAACTGGGCGAAGAAGCGAAGCGTGAAGG           >  SRR19973061.3417577/1‑150 (MQ=60)
                                                                                                                                            TCGCGGTATAGACAACGACTTCCGTGATGTAAACGACTGGCAGTTCTTCAAAGGTGCGCAGAAGCTGGGCGAACTGGGTCAGCCGGTGCTGGTGCACTGCGAAAACGCGCTGATTTGTGACGAACTGGGCGAAGAAGCGAAGCGTGAAGG           >  SRR19973061.3417542/1‑150 (MQ=60)
                                                                                                                                             CGCGGTATAGACAACGACTTCCGTGATGTAAACGACTGGCAGTTCTTCAAAGGTGCGCAGAAGCTGGGCGAACTGGGTCAGCCGGTGCTGGTGCACTGCGAAAACGCGCTGATTTGTGACGAACTGGGCGAAGAAGCGAAGCGTGAAGGT          <  SRR19973061.2681231/150‑1 (MQ=60)
                                                                                                                                               CGGTATAGACAACGACTTCCGTGATGTAAACGACTGGCAGTTCTTCAAAGGTGCGCAGAAGCTGGGCGAACTGGGTCAGCCGGTGCTGGTGCACTGCGAAAACGCGCTGATTTGTGACGAACTGGGCGAAGAAGCGAAGCGTGAAGGTCG        >  SRR19973061.482382/1‑150 (MQ=60)
                                                                                                                                                 GTATAGACAACGACTTCCGTGATGTAAACGACTGGCAGTTCTTCAAAGGTGCGCAGAAGCTGGGCGAACTGGGTCAGCCGGTGCTGGTGCACTGCGAAAACGCGCTGATTTGTGACGAACTGGGCGAAGAAGCGAAGCGTGAAGGTCGCG      >  SRR19973061.271586/1‑150 (MQ=60)
                                                                                                                                                  TATAGACAACGACTTCCGTGATGTAAACGACTGGCAGTTCTTCAAAGGTGCGCAGAAGCTGGGCGAACTGGGTCAGCCGGTGCTGGTGCACTGCGAAAACGCGCTGATTTGTGACGAACTGGGCGAAGAAGCGAAGCGTGAAGGTCGCGT     <  SRR19973061.1247374/150‑1 (MQ=60)
                                                                                                                                                     AGACAACGACTTCCGTGATGTAAACGACTGGCAGTTCTTCAAAGGTGCGCAGAAGCTGGGCGAACTGGGTCAGCCGGTGCTGGTGCACTGCGAAAACGCGCTGATTTGTGACGAACTGGGCGAAGAAGCGAAGCGTGAAGGTCGCGTAAC  >  SRR19973061.1385209/1‑150 (MQ=60)
                                                                                                                                                     AGACAACGACTTCCGTGATGTAAACGACTGGCAGTTCTTCAAAGGTGCGCAGAAGCTGGGCGAACTGGGTCAGCCGGTGCTGGTGCACTGCGAAAACGCGCTGATTTGTGACGAACTGGGCGAAGAAGCGAAGCGTGAAGGTCGCGTAAC  <  SRR19973061.1703566/150‑1 (MQ=60)
                                                                                                                                                     AGACAACGACTTCCGTGATGTAAACGACTGGCAGTTCTTCAAAGGTGCGCAGAAGCTGGGCGAACTGGGTCAGCCGGTGCTGGTGCACTGCGAAAACGCGCTGATTTGTGACGAACTGGGCGAAGAAGCGAAGCGTGAAGGTCGCGTAAC  >  SRR19973061.882451/1‑150 (MQ=60)
                                                                                                                                                     |                                                                                                                                                     
AAGTTCGATGCCGCTAAAGGCAAGCTGACTATTGATGCGGCACAACTCGGTGGCCTGGTGTCTTACAACATCGACCGTCTGCATGAGCTGGATGAAGTGGGCGTTGTCGGCTTCAAATGCTTCGTTGCGACCTGTGGCGATCGCGGTATCGACAACGACTTCCGTGATGTAAACGACTGGCAGTTCTTCAAAGGTGCGCAGAAGCTGGGCGAACTGGGTCAGCCGGTGCTGGTGCACTGCGAAAACGCGCTGATTTGTGACGAACTGGGCGAAGAAGCGAAGCGTGAAGGTCGCGTAAC  >  CP009273/534924‑535222

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑