Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F534 I1 R1 1235 230.2 7611948 95.1% 7238962 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009273 582,322 C→A E15* (GAA→TAA)  envY ← porin thermoregulatory transcriptional activator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273582,3220CA99.5% 615.6 / NA 187E15* (GAA→TAA) envYporin thermoregulatory transcriptional activator
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base A (105/81);  total (106/81)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 187 total aligned reads displayed.

ATGATGTTACGGTTGATGTGGGTGATTAATACGTGATTTAACGTTGAGAGTTCAATGACGTTATTGTTGCACGCGAAGGCGGCCAGGTAGTTTTTCGGAAGGGTAAACGTAGCATGGTTATTGACGCTTACCTCTACCTCTTTTTCGGTCAGGATCACCACGCAAGGTTCACTGCTGCTCAATTGCATTTCGCACTCCTCAGATATCAGAAACTCCGCTCAAAGGATCTATGCTTCCTGCATGAGTGATCGGCCCGTTCGCCGATAACGATCTTCTTTCTTTAGCACGCTTT  >  CP009273/582177‑582468
                                                                                                                                                 |                                                                                                                                                  
atgatgTTACGGTTGATGTGGGTGATTAATACGTGATTTAACGTTGAGAGTTCAATGACGTTATTGTTGCACGCGAAGGCGGCCAGGTAGTTTTTCGGAAGGGTAAACGTAGCATGGTTATTGACGCTTACCTCTACCTCTTTTTAGGTc                                                                                                                                                <  2:2721860/150‑1 (MQ=255)
 tgatgTTACGGTTGATGTGGGTGATTAATACGTGATTTAACGTTGAGAGTTCAATGACGTTATTGTTGCACGCGAAGGCGGCCAGGTAGTTTTTCGGAAGGGTAAACGTAGCATGGTTATTGACGCTTACCTCTACCTCTTTTTAGGTCa                                                                                                                                               <  2:1391431/150‑1 (MQ=255)
 tgatgTTACGGTTGATGTGGGTGATTAATACGTGATTTAACGTTGAGAGTTCAATGACGTTATTGTTGCACGCGAAGGCGGCCAGGTAGTTTTTCGGAAGGGTAAACGTAGCATGGTTATTGACGCTTACCTCTACCTCTTTTTAGGTCa                                                                                                                                               >  2:450884/1‑150 (MQ=255)
   atgTTACGGTTGATGTGGGTGATTAATACGTGATTTAACGTTGAGAGTTCAATGACGTTATTGTTGCACGCGAAGGCGGCCAGGTAGTTTTTCGGAAGGGTAAACGTAGCATGGTTATTGACGCTTACCTCTACCTCTTTTTAGGTCAgg                                                                                                                                             >  1:3040068/1‑150 (MQ=255)
    tgTTACGGTTGATGTGGGTGATTAATACGTGATTTAACGTTGAGAGTTCAATGACGTTATTGTTGCACGCGAAGGCGGCCAGGTAGTTTTTCGGAAGGGTAAACGTAGCATGGTTATTGACGCTTACCTCTACCTCTTTTTAGGTCAGGa                                                                                                                                            <  1:3526681/150‑1 (MQ=255)
    tgTTACGGTTGATGTGGGTGATTAATACGTGATTTAACGTTGAGAGTTCAATGACGTTATTGTTGCACGCGAAGGCGGCCAGGTAGTTTTTCGGAAGGGTAAACGTAGCATGGTTATTGACGCTTACCTCTACCTCTTTTTAGGTCAGGa                                                                                                                                            >  2:648086/1‑150 (MQ=255)
           gTTGATGTGGGTGATTAATACGTGATTTAACGTTGAGAGTTCAATGACGTTATTGTTGCACGCGAAGGCGGCCAGGTAGTTTTTCGGAAGGGTAAACGTAGCATGGTTATTGACGCTTACCTCTACCTCTTTTTAGGTCAGGATcaccac                                                                                                                                     <  1:3509550/150‑1 (MQ=255)
            ttGATGTGGGTGATTAATACGTGATTTAACGTTGAGAGTTCAATGACGTTATTGTTGCACGCGAAGGCGGCCAGGTAGTTTTTCGGAAGGGTAAACGTAGCATGGTTATTGACGCTTACCTCTACCTCTTTTTAGGTCAGGATCACCACg                                                                                                                                    <  1:1056580/150‑1 (MQ=255)
                 gtgGGTGATTAATACGTGATTTAACGTTGAGAGTTCAATGACGTTATTGTTGCACGCGAAGGCGGCCAGGTAGTTTTTCGGAAGGGTAAACGTAGCATGGTTATTGACGCTTACCTCTACCTCTTTTTAGGTCAGGATCACCACGCAAgg                                                                                                                               <  2:1861347/150‑1 (MQ=255)
                 gtgGGTGATTAATACGTGATTTAACGTTGAGAGTTCAATGACGTTATTGTTGCACGCGAAGGCGGCCAGGTAGTTTTTCGGAAGGGTAAACGTAGCATGGTTATTGACGCTTACCTCTACCTCTTTTTAGGTCAGGATCACCACGCAAgg                                                                                                                               <  2:2174826/150‑1 (MQ=255)
                   gngTGATTAATACGTGATTTAACGTTGAGAGTTCAATGACGTTATTGTTGCACGCGAAGGCGGCCAGGTAGTTTTTCGGAAGGGTAAACGTAGCATGGTTATTGACGCTTACCTCTACCTCTTTTTAGGTCAGGATCACCACGCAAGGtt                                                                                                                             >  1:2528739/1‑150 (MQ=255)
                     gTGATTAATACGTGATTTAACGTTGAGAGTTCAATGACGTTATTGTTGCACGCGAAGGCGGCCAGGTAGTTTTTCGGAAGGGTAAACGTAGCATGGTTATTGACGCTTACCTCTACCTCTTTTTAGGTCAGGATCACCACGCAAGGTTCa                                                                                                                           >  2:1244599/1‑150 (MQ=255)
                       gATTAATACGTGATTTAACGTTGAGAGTTCAATGACGTTATTGTTGCACGCGAAGGCGGCCAGGTAGTTTTTCGGAAGGGTAAACGTAGCATGGTTATTGACGCTTACCTCTACCTCTTTTTAGGTCAGGATCACCACGCAAGGTTCAct                                                                                                                         <  1:1170443/150‑1 (MQ=255)
                        aTTAATACGTGATTTAACGTTGAGAGTTCAATGACGTTATTGTTGCACGCGAAGGCGGCCAGGTAGTTTTTCGGAAGGGTAAACGTAGCATGGTTATTGACGCTTACCTCTACCTCTTTTTAGGTCAGGATCACCACGCAAGGTTCActg                                                                                                                        >  1:2013055/1‑150 (MQ=255)
                        aTTAATACGTGATTTAACGTTGAGAGTTCAATGACGTTATTGTTGCACGCGAAGGCGGCCAGGTAGTTTTTCGGAAGGGTAAACGTAGCATGGTTATTGACGCTTACCTCTACCTCTTTTTAGGTCAGGATCACCACGCAAGGTTCActg                                                                                                                        >  1:1872515/1‑150 (MQ=255)
                          tAATACGTGATTTAACGTTGAGAGTTCAATGACGTTATTGTTGCACGCGAAGGCGGCCAGGTAGTTTTTCGGAAGGGTAAACGTAGCATGGTTATTGACGCTTACCTCTACCTCTTTTTAGGTCAGGATCACCACGCAAGGTTCActgct                                                                                                                      >  1:611740/1‑150 (MQ=255)
                           aaTACGTGATTTAACGTTGAGAGTTCAATGACGTTATTGTTGCACGCGAAGGCGGCCAGGTAGTTTTTCGGAAGGGTAAACGTAGCATGGTTATTGACGCTTACCTCTACCTCTTTTTAGGTCAGGATCACCACGCAAGGTTCActgctg                                                                                                                     <  2:2104033/150‑1 (MQ=255)
                            aTACGTGATTTAACGTTGAGAGTTCAATGACGTTATTGTTGCACGCGAAGGCGGCCAGGTAGTTTTTCGGAAGGGTAAACGTAGCATGGTTATTGACGCTTACCTCTACCTCTTTTTAGGTCAGGATCACCACGCAAGGTTCACtgctgc                                                                                                                    >  2:2681286/1‑150 (MQ=255)
                            aTACGTGATTTAACGTTGAGAGTTCAATGACGTTATTGTTGCACGCGAAGGCGGCCAGGTAGTTTTTCGGAAGGGTAAACGTAGCATGGTTATTGACGCTTACCTCTACCTCTTTTTAGGTCAGGATCACCACGCAAGGTTCACtgctgc                                                                                                                    >  1:3771228/1‑150 (MQ=255)
                            aTACGTGATTTAACGTTGAGAGTTCAATGACGTTATTGTTGCACGCGAAGGCGGCCAGGTAGTTTTTCGGAAGGGTAAACGTAGCATGGTTATTGACGCTTACCTCTACCTCTTTTTAGGTCAGGATCACCACGCAAGGTTCACtgctgc                                                                                                                    >  1:3549138/1‑150 (MQ=255)
                             tACGTGATTTAACGTTGAGAGTTCAATGACGTTATTGTTGCACGCGAAGGCGGCCAGGTAGTTTTTCGGAAGGGTAAACGTAGCATGGTTATTGACGCTTACCTCTACCTCTTTTTAGGTCAGGATCACCACGCAAGGTTCACTgctgct                                                                                                                   <  1:863115/150‑1 (MQ=255)
                             tACGTGATTTAACGTTGAGAGTTCAATGACGTTATTGTTGCACGCGAAGGCGGCCAGGTAGTTTTTCGGAAGGGTAAACGTAGCATGGTTATTGACGCTTACCTCTACCTCTTTTTAGGTCAGGATCACCACGCAAGGTTCACTgctgct                                                                                                                   <  2:2225094/150‑1 (MQ=255)
                              aCGTGATTTAACGTTGAGAGTTCAATGACGTTATTGTTGCACGCGAAGGCGGCCAGGTAGTTTTTCGGAAGGGTAAACGTAGCATGGTTATTGACGCTTACCTCTACCTCTTTTTAGGTCAGGATCACCACGCAAGGTTCACTGCTGCTc                                                                                                                  <  2:1343907/150‑1 (MQ=255)
                                gTGATTTAACGTTGAGAGTTCAATGACGTTATTGTTGCACGCGAAGGCGGCCAGGTAGTTTTTCGGAAGGGTAAACGTAGCATGGTTATTGACGCTTACCTCTACCTCTTTTTAGGTCAGGATCACCACGCAAGGTTCACTGCTGCTCaa                                                                                                                <  1:1244599/150‑1 (MQ=255)
                                 tGATTTAACGTTGAGAGTTCAATGACGTTATTGTTGCACGCGAAGGCGGCCAGGTAGTTTTTCGGAAGGGTAAACGTAGCATGGTTATTGACGCTTACCTCTACCTCTTTTTAGGTCAGGATCACCACGCAAGGTTCACTGCTGCTCAAt                                                                                                               <  2:687123/150‑1 (MQ=255)
                                   aTTTAACGTTGAGAGTTCAATGACGTTATTGTTGCACGCGAAGGCGGCCAGGTAGTTTTTCGGAAGGGTAAACGTAGCATGGTTATTGACGCTTACCTCTACCTCTTTTTAGGTCAGGATCACCACGCAAGGTTCACTGCTGCTCAATTg                                                                                                             >  2:449016/1‑150 (MQ=255)
                                      tAACGTTGAGAGTTCAATGACGTTATTGTTGCACGCGAAGGCGGCCAGGTAGTTTTTCGGAAGGGTAAACGTAGCATGGTTATTGACGCTTACCTCTACCTCTTTTTAGGTCAGGATCACCACGCAAGGTTCACTGCTGCTCAATTGCAt                                                                                                          <  2:3335511/150‑1 (MQ=255)
                                       aaCGTTGAGAGTTCAATGACGTTATTGTTGCACGCGAAGGCGGCCAGGTAGTTTTTCGGAAGGGTAAACGTAGCATGGTTATTGACGCTTACCTCTACCTCTTTTTAGGTCAGGATCACCACGCAAGGTTCACTGCTGCTCAATTGCAtt                                                                                                         <  1:1343309/150‑1 (MQ=255)
                                       aaCGTTGAGAGTTCAATGACGTTATTGTTGCACGCGAAGGCGGCCAGGTAGTATTTCGGAAGGGTAAACGTAGCATGGTTATTGACGCTTACCTCTACCTCTTTTTAGGTCAGGATCACCACGCAAGGTTCACTGCTGCTCAATTGCAtt                                                                                                         >  1:2118107/1‑150 (MQ=255)
                                          gTTGAGAGTTCAATGACGTTATTGTTGCACGCGAAGGCGGCCAGGTAGTTTTTCGGAAGGGTAAACGTAGCATGGTTATTGACGCTTACCTCTACCTCTTTTTAGGTCAGGATCACCACGCAAGGTTCACTGCTGCTCAATTGCATTTCg                                                                                                      >  2:3311779/1‑150 (MQ=255)
                                          gTTGAGAGTTCAATGACGTTATTGTTGCACGCGAAGGCGGCCAGGTAGTTTTTCGGAAGGGTAAACGTAGCATGGTTATTGACGCTTACCTCTACCTCTTTTTAGGTCAGGATCACCACGCAAGGTTCACTGCTGCTCAATTGCATTTCg                                                                                                      >  1:2261065/1‑150 (MQ=255)
                                           ttGAGAGTTCAATGACGTTATTGTTGCACGCGAAGGCGGCCAGGTAGTTTTTCGGAAGGGTAAACGTAGCATGGTTATTGACGCTTACCTCTACCTCTTTTTAGGTCAGGATCACCACGCAAGGTTCACTGCTGCTCAATTGCATTTCGc                                                                                                     >  1:278963/1‑150 (MQ=255)
                                           ttGAGAGTTCAATGACGTTATTGTTGCACGCGAAGGCGGCCAGGTAGTTTTTCGGAAGGGTAAACGTAGCATGGTTATTGACGCTTACCTCTACCTCTTTTTAGGTCAGGATCACCACGCAAGGTTCACTGCTGCTCAATTGCATTTCGc                                                                                                     >  2:2810052/1‑150 (MQ=255)
                                                   tCAATGACGTTATTGTTGCACGCGAAGGCGGCCAGGTAGTTTTTCGGAAGGGTAAACGTAGCATGGTTATTGACGCTTACCTCTACCTCTTTTTAGGTCAGGATCACCACGCAAGGTTCACTGCTGCTCAATTGCATTTCGCACTCCTCa                                                                                             <  1:73282/150‑1 (MQ=255)
                                                   tCAATGACGTTATTGTTGCACGCGAAGGCGGCCAGGTAGTTTTTCGGAAGGGTAAACGTAGCATGGTTATTGACGCTTACCTCTACCTCTTTTTAGGTCAGGATCACCACGCAAGGTTCACTGCTGCTCAATTGCATTTCGCACTCCTCa                                                                                             <  1:74601/150‑1 (MQ=255)
                                                    cAATGACGTTATTGTTGCACGCGAAGGCGGCCAGGTAGTTTTTCGGAAGGGTAAACGTAGCATGGTTATTGACGCTTACCTCTACCTCTTTTTAGGTCAGGATCACCACGCAAGGTTCACTGCTGCTc                                                                                                                  <  1:561041/128‑1 (MQ=255)
                                                    cAATGACGTTATTGTTGCACGCGAAGGCGGCCAGGTAGTTTTTCGGAAGGGTAAACGTAGCATGGTTATTGACGCTTACCTCTACCTCTTTTTAGGTCAGGATCACCACGCAAGGTTCACTGCTGCTc                                                                                                                  >  2:561041/1‑128 (MQ=255)
                                                     aaTGACGTTATTGTTGCACGCGAAGGCGGCCAGGTAGTTTTTCGGAAGGGTAAACGTAGCATGGTTATTGACGCTTACCTCTACCTCTTTTTAGGTCAGGATCACCACGCAAGGTTCACTGCTGCTCAATTGCATTTCGCACTCCTCAGa                                                                                           >  2:283533/1‑150 (MQ=255)
                                                      aTGACGTTATTGTTGCACGCGAAGGCGGCCAGGTAGTTTTTCGGAAGGGTAAACGTAGCATGGTTATTGACGCTTACCTCTACCTCTTTTTAGGTCAGGATCACCACGCAAGGTTCACTGCTGCTCAATTGCATTTCGCACTCCTCAGat                                                                                          >  1:1759614/1‑150 (MQ=255)
                                                       tGACGTTATTGTTGCACGCGAAGGCGGCCAGGTAGTTTTTCGGAAGGGTAAACGTAGCATGGTTATTGACGCTTACCTCTACCTCTTTTTAGGTCAGGATCACCACGCAAGGTTCACTGCTGCTCAATTGCATTTCGCACTCCTCAGata                                                                                         <  1:3614123/150‑1 (MQ=255)
                                                        gACGTTATTGTTGCACGCGAAGGCGGCCAGGTAGTTTTTCGGAAGGGTAAACGTAGCATGGTTATTGACGCTTACCTCTACCTCTTTTTAGGTCAGGATCACCACGCAAGGTTCACTGCTGCTCAATTGCATTTCGCACTCCTCAGatat                                                                                        >  1:2316401/1‑150 (MQ=255)
                                                        gACGTTATTGTTGCACGCGAAGGCGGCCAGGTAGTTTTTCGGAAGGGTAAACGTAGCATGGTTATTGACGCTTACCTCTACCTCTTTTTAGGTCAGGATCACCACGCAAGGTTCACTGCTGCTCAATTGCATTTCGCACTCCTCAGatat                                                                                        >  1:410025/1‑150 (MQ=255)
                                                           gTTATTGTTGCACGCGAAGGCGGCCAGGTAGTTTTTCGGAAGGGTAAACGTAGCATGGTTATTGACGCTTACCTCTACCTCTTTTTAGGTCAGGATCACCACGCAAGGTTCACTGCTGCTCAATTGCATTTCGCACTCCTCAGATATCAg                                                                                     >  1:2712226/1‑150 (MQ=255)
                                                             tATTGTTGCACGCGAAGGCGGCCAGGTAGTTTTTCGGAAGGGTAAACGTAGCATGGTTATTGACGCTTACCTCTACCTCTTTTTAGGTCAGGATCACCACGCAAGGTTCACTGCTGCTCAATTGCATTTCGCACTCCTCAGATATCAGaa                                                                                   >  1:2164598/1‑150 (MQ=255)
                                                             tATTGTTGCACGCGAAGGCGGCCAGGTAGTTTTTCGGAAGGGTAAACGTAGCATGGTTATTGACGCTTACCTCTACCTCTTTTTAGGTCAGGATCACCACGCAAGGTTCACTGCTGCTCAATTGCATTTCGCACTCCTCAGATATCAGaa                                                                                   >  2:3699525/1‑150 (MQ=255)
                                                             tATTGTTGCACGCGAAGGCGGCCAGGTAGTTTTTCGGAAGGGTAAACGTAGCATGGTTATTGACGCTTACCTCTACCTCTTTTTAGGTCAGGATCACCACGCAAGGTTCACTGCTGCTCAATTGCATTTCGCACTCCTCAGATATCAGaa                                                                                   >  1:989164/1‑150 (MQ=255)
                                                              aTTGTTGCACGCGAAGGCGGCCAGGTAGTTTTTCGGAAGGGTAAACGTAGCATGGTTATTGACGCTTACCTCTACCTCTTTTTAGGTCAGGATCACCACGCAAGGTTCACTGCTGCTCAATTGCATTTCGCACTCCTCAGATATCAGaaa                                                                                  >  2:850060/1‑150 (MQ=255)
                                                               ttgttgCACGCGAAGGCGGCCAGGTAGTTTTTCGGAAGGGTAAACGTAGCATGGTTATTGACGCTTACCTCTACCTCTTTTTAGGTCAGGATCACCACGCAAGGTTCACTGCTGCTCAATTGCATTTCGCACTCCTCAGATATCAGAAAc                                                                                 <  2:1695942/150‑1 (MQ=255)
                                                                tgttgCACGCGAAGGCGGCCAGGTAGTTTTTCGGAAGGGTAAACGTAGCATGGTTATTGACGCTTACCTCTACCTCTTTTTAGGTCAGGATCACCACGCAAGGTTCACTGCTGCTCAATTGCATTTCGCACTCCTCAGATATCAGAAACt                                                                                <  2:3165140/150‑1 (MQ=255)
                                                                tgttgCACGCGAAGGCGGCCAGGTAGTTTTTCGGAAGGGTAAACGTAGCATGGTTATTGACGCTTACCTCTACCTCTTTTTAGGTCAGGATCACCACGCAAGGTTCACTGCTGCTCAATTGCATTTCGCACTCCTCAGATATCAGAAACt                                                                                >  2:2303347/1‑150 (MQ=255)
                                                                  ttgCACGCGAAGGCGGCCAGGTAGTTTTTCGGAAGGGTAAACGTAGCATGGTTATTGACGCTTACCTCTACCTCTTTTTAGGTCAGGATCACCACGCAAGGTTCACTGCTGCTCAATTGCATTTCGCACTCCTCAGATATCAGAAACTcc                                                                              >  2:2583417/1‑150 (MQ=255)
                                                                         cgAAGGCGGCCAGGTAGTTTTTCGGAAGGGTAAACGTAGCATGGTTATTGACGCTTACCTCTACCTCTTTTTAGGTCAGGATCACCACGCAAGGTTCACTGCTGCTCAATTGCATTTCGCACTCCTCAGATATCAGAAACTCCGCTCaaa                                                                       >  2:3582737/1‑150 (MQ=255)
                                                                         cgAAGGCGGCCAGGTAGTTTTTCGGAAGGGTAAACGTAGCATGGTTATTGACGCTTACCTCTACCTCTTTTTAGGTCAGGATCACCACGCAAGGTTCACTGCTGCTCAATTGCATTTCGCACTCCTCAGATATCAGAAACTCCGCTCaaa                                                                       >  1:3321562/1‑150 (MQ=255)
                                                                          gAAGGCGGCCAGGTAGTTTTTCGGAAGGGTAAACGTAGCATGGTTATTGACGCTTACCTCTACCTCTTTTTAGGTCAGGATCACCACGCAAGGTTCACTGCTGCTCAATTGCATTTCGCACTCCTCAGATATCAGAAACTCCGCTCAAAg                                                                      >  1:283671/1‑150 (MQ=255)
                                                                               cggcCAGGTAGTTTTTCGGAAGGGTAAACGTAGCATGGTTATTGACGCTTACCTCTACCTCTTTTTAGGTCAGGATCACCACGCAAGGTTCACTGCTGCTCAATTGCATTTCGCACTCCTCAGATATCAGAAACTCCGCTCAAAGGATCt                                                                 >  2:1237318/1‑150 (MQ=255)
                                                                               cggcCAGGTAGTTTTTCGGAAGGGTAAACGTAGCATGGTTATTGACGCTTACCTCTACCTCTTTTTAGGTCAGGATCACCACGCAAGGTTCACTGCTGCTCAATTGCATTTCGCACTCCTCAGATATCAGAAACTCCGCTCAAAGGATCt                                                                 >  2:3138016/1‑150 (MQ=255)
                                                                                ggcCAGGTAGTTTTTCGGAAGGGTAAACGTAGCATGGTTATTGACGCTTACCTCTACCTCTTTTTAGGTCAGGATCACCACGCAAGGTTCACTGCTGCTCAATTGCATTTCGCACTCCTCAGATATCAGAAACTCCGCTCAAAGGATCTa                                                                >  1:2276541/1‑150 (MQ=255)
                                                                                ggcCAGGTAGTTTTTCGGAAGGGTAAACGTAGCATGGTTATTGACGCTTACCTCTACCTCTTTTTAGGTCAGGATCACCACGCAAGGTTCACTGCTGCTCAATTGCATTTCGCACTCCTCAGATATCAGAAACTCCGCTCAAAGGATCTa                                                                <  2:2397112/150‑1 (MQ=255)
                                                                                 gcCAGGTAGTTTTTCGGAAGGGTAAACGTAGCATGGTTATTGACGCTTACCTCTACCTCTTTTTAGGTCAGGATCACCACGCAAGGTTCACTGCTGCTCAATTGCATTTCGCACTCCTCAGATATCAGAAACTCCGCTCAAAGGATCTAt                                                               <  1:648086/150‑1 (MQ=255)
                                                                                   cAGGTAGTTTTTCGGAAGGGTAAACGTAGCATGGTTATTGACGCTTACCTCTACCTCTTTTTAGGTCAGGATCACCACGCAAGGTTCACTGCTGCTCAATTGCATTTCGCACTCCTCAGATATCAGAAACTCCGCTCAAAGGATCTATGc                                                             >  1:2961946/1‑150 (MQ=255)
                                                                                    aGGTAGTTTTTCGGAAGGGTAAACGTAGCATGGTTATTGACGCTTACCTCTACCTCTTTTTAGGTCAGGATCACCACGCAAGGTTCACTGCTGCTCAATTGCATTTCGCACTCCTCAGATATCAGAAACTCCGCTCAAAGGATCTATGCt                                                            >  2:570304/1‑150 (MQ=255)
                                                                                     ggTAGTTTTTCGGAAGGGTAAACGTAGCATGGTTATTGACGCTTACCTCTACCTCTTTTTAGGTCAGGATCACCACGCAAGGTTCACTGCTGCTCAATTGCATTTCGCACTCCTCAGATATCAGAAACTCCGCTCAAAGGATCTATGCtt                                                           >  1:264547/1‑150 (MQ=255)
                                                                                      gTAGTTTTTCGGAAGGGTAAACGTAGCATGGTTATTGACGCTTACCTCTACCTCTTTTTAGGTCAGGATCACCACGCAAGGTTCACTGCTGCTCAATTGCATTTCGCACTCCTCAGATATCAGAAACTCCGCTCAAAGGATCTATGCTTc                                                          <  1:833478/150‑1 (MQ=255)
                                                                                          tttttCGGAAGGGTAAACGTAGCATGGTTATTGACGCTTACCTCTACCTCTTTTTAGGTCAGGATCACCACGCAAGGTTCACTGCTGCTCAATTGCATTTCGCACTCCTCAGATATCAGAAACTCCGCTCAAAGGATCTATGCTTCCTGc                                                      <  1:3465242/150‑1 (MQ=255)
                                                                                          tttttCGGAAGGGTAAACGTAGCATGGTTATTGACGCTTACCTCTACCTCTTTTTAGGTCAGGATCACCACGCAAGGTTCACTGCTGCTCAATTGCATTTCGCACTCCTCAGATATCAGAAACTCCGCTCAAAGGATCTATGCTTCCTGc                                                      >  2:2449284/1‑150 (MQ=255)
                                                                                                ggAAGGGTAAACGTAGCATGGTTATTGACGCTTACCTCTACCTCTTTTTAGGTCAGGATCACCACGCAAGGTTCACTGCTGCTCAATTGCATTTCGCACTCCTCAGATATCAGAAACTCCGCTCAAAGGATCTATGCTTCCTGCATGAGt                                                >  1:3100531/1‑150 (MQ=255)
                                                                                                 gAAGGGTAAACGTAGCATGGTTATTGACGCTTACCTCTACCTCTTTTTAGGTCAGGATCACCACGCAAGGTTCACTGCTGCTCAATTGCATTTCGCACTCCTCAGATATCAGAAACTCCGCTCAAAGGATCTATGCTTCCTGCATGAGTg                                               <  2:1875118/150‑1 (MQ=255)
                                                                                                   aGGGTAAACGTAGCATGGTTATTGACGCTTACCTCTACCTCTTTTTAGGTCAGGATCACCACGCAAGGTTCACTGCTGCTCAATTGCATTTCGCACTCCTCAGATATCAGAAACTCCGCTCAAAGGATCTATGCTTCCTGCATGAGTGAt                                             >  1:1116971/1‑150 (MQ=255)
                                                                                                   aGGGTAAACGTAGCATGGTTATTGACGCTTACCTCTACCTCTTTTTAGGTCAGGATCACCACGCAAGGTTCACTGCTGCTCAATTGCATTTCGCACTCCTCAGATATCAGAAACTCCGCTCAAAGGATCTATGCTTCCTGCATGAGTGAt                                             >  2:1307693/1‑150 (MQ=255)
                                                                                                   aGGGTAAACGTAGCATGGTTATTGACGCTGACCTCTACCTCTTTTTAGGTCAGGATCACCACGCAAGGTTCACTGCTGCTCAATTGCATTTCGCACTCCTCAGATATCAGAAACTCCGCTCAAAGGATCTATGCTTCCTGCATGAGTGAt                                             <  2:3478807/150‑1 (MQ=255)
                                                                                                     ggTAAACGTAGCCTGGTTATTGACGCTTACCTCTACCTCTTTTTAGGTCAGGATCACCACGCAAGGTTCACTGCTGCTCAATTGCATTTCGCACTCCTCAGATATCAGAAACTCCGCTCAAAGGATCTATGCTTCCTGCATGAGTGATCg                                           <  2:3199914/150‑1 (MQ=255)
                                                                                                     ggTAAACGTAGCATGGTTATTGACGCTTACTTCTACCTCTTTTTAGGTCAGGATCACCACGCAAGGTTCACTGCTGCTCAATTGCATTTCGCACTCCTCAGATATCAGAAACTCCGCTCAAAGGATCTATGCTTCCTGCATGAGTGATCg                                           >  2:354841/1‑150 (MQ=255)
                                                                                                     ggTAAACGTAGCATGGTTATTGACGCTTACCTCTACCTCTTTTTAGGTCAGGATCACCACGCAAGGTTCACTGCTGCTCAATTGCATTTCGCACTCCTCAGATATCAGAAACTCCGCTCAAAGGATCTATGCTTCCTGCATGAGTGATCg                                           >  2:410587/1‑150 (MQ=255)
                                                                                                         aaCGTAGCATGGTTATTGACGCTTACCTCTACCTCTTTTTAGGTCAGGATCACCACGCAAGGTTCACTGCTGCTCAATTGCATTTCGCACTCCTCAGATATCAGAAACTCCGCTCAAAGGATCTATGCTTCCTGCATGAGTGATCGGccc                                       >  2:1304046/1‑150 (MQ=255)
                                                                                                           cGTAGCATGGTTATTGACGCTTACCTCTACCTCTTTTTAGGTCAGGATCACCACGCAAGGTTCACTGCTGCTCAATTGCATTTCGCACTCCTCAGATATCAGAAACTCCGCTCAAAGGATCTATGCTTCCTGCATGAGTGATCGGCCCGt                                     <  2:2261065/150‑1 (MQ=255)
                                                                                                            gTAGCATGGTTATTGACGCTTACCTCTACCTCTTTTTAGGTCAGGATCACCACGCAAGGTTCACTGCTGCTCAATTGCATTTCGCACTCCTCAGATATCAGAAACTCCGCTCAAAGGATCTATGCTTCCTGCATGAGTGATCGGCCCGtt                                    >  2:3095781/1‑150 (MQ=255)
                                                                                                            gTAGCATGGTTATTGACGCTTACCTCTACCTCTTTTTAGGTCAGGATCACCACGCAAGGTTCACTGCTGCTCAATTGCATTTCGCACTCCTCAGATATCAGAAACTCCGCTCAAAGGATCTATGCTTCCTGCATGAGTGATCGGCCCGtt                                    >  2:1706277/1‑150 (MQ=255)
                                                                                                             tAGCATGGTTATTGACGCTTACCTCTACCTCTTTTTAGGTCAGGATCACCACGCAAGGTTCACTGCTGCTCAATTGCATTTCGCACTCCTCAGATATCAGAAACTCCGCTCAAAGGATCTATGCTTCCTGCATGAGTGATCGGCCCGTTc                                   <  2:298603/150‑1 (MQ=255)
                                                                                                             tAGCATGGTTATTGACGCTTACCTCTACCTCTTTTTAGGTCAGGATCACCACGCAAGGTTCACTGCTGCTCAATTGCATTTCGCACTCCTCAGATATCAGAAACTCCGCTCAAAGGATCTATGCTTCCTGCATGAGTGATCGGCCCGTTc                                   <  2:416335/150‑1 (MQ=255)
                                                                                                                      tATTGACGCTTACCTCTACCTCTTTTTAGGTCAGGATCACCACGCAAGGTTCACTGCTGCTCAATTGCATTTCGCACTCCTCAGATATCAGAAACTCCGCTCAAAGGATCTATGCTTCCTGCATGAGTGATCGGCCCGTTCGCCGATAAc                          >  1:1610649/1‑150 (MQ=255)
                                                                                                                      tATTGACGCTTACCTCTACCTCTTTTTAGGTCAGGATCACCACGCAAGGTTCACTGCTGCTCAATTGCATTTCGCACTCCTCAGATATCAGAAACTCCGCTCAAAGGATCTATGCTTCCTGCATGAGTGATCGGCCCGTTCGCCGATAAc                          <  2:3529258/150‑1 (MQ=255)
                                                                                                                         tGACGCTTACCTCTACCTCTTTTTAGGTCAGGATCACCACGCAAGGTTCACTGCTGCTCAATTGCATTTCGCACTCCTCAGATATCAGAAACTCCGCTCAAAGGATCTATGCTTCCTGCATGAGTGATCGGCCCGTTCGCCGATAACGAt                       <  2:1942267/150‑1 (MQ=255)
                                                                                                                         tGACGCTTACCTCTACCTCTTTTTAGGTCAGGATCACCACGCAAGGTTCACTGCTGCTCAATTGCATTTCGCACTCCTCAGATATCAGAAACTCCGCTCAAAGGATCTATGCTTCCTGCATGAGTGATCGGCCCGTTCGCCGATAACGAt                       <  1:450884/150‑1 (MQ=255)
                                                                                                                         tGACGCTTACCTCTACCTCTTTTTAGGTCAGGATCACCACGCAAGGTTCACTGCTGCTCAATTGCATTTCGCACTCCTCAGATATCAGAAACTCCGCTCAAAGGATCTATGCTTCCTGCATGAGTGATCGGCCCGTTCGCCGATAACGAt                       >  1:3174357/1‑150 (MQ=255)
                                                                                                                         tGACGCTTACCTCTACCTCTTTTTAGGTCAGGATCACCACGCAAGGTTCACTGCTGCTCAATTGCATTTCGCACTCCTCAGATATCAGAAACTCCGCTCAAAGGATCTATGCTTCCTGCATGAGTGATCGGCCCGTTCGCCGATAACGAt                       >  1:896026/1‑150 (MQ=255)
                                                                                                                         tGACGCTGACCTCTACCTCTTTTTAGGTCAGGATCACCACGCAAGGTTCACTGCTGCTCAATTGCATTTCGCACTCCTCAGATATCAGAAACTCCGCTCAAAGGATCTATGCTTCCTGCATGAGTGATCGGCCCGTTCGCCGATAACGAt                       <  2:644217/150‑1 (MQ=255)
                                                                                                                          gACGCTTACCTCTACCTCTTTTTAGGTCAGGATCACCACGCAAGGTTCACTGCTGCTCAATTGCATTTCGCACTCCTCAGATATCAGAAACTCCGCTCAAAGGATCTATGCTTCCTGCATGAGTGATCGGCCCGTTCGCCGATAACGAtc                      <  2:2169725/150‑1 (MQ=255)
                                                                                                                            cGCTTACCTCTACCTCTTTTTAGGTCAGGATCACCACGCAAGGTTCACTGCTGCTCAATTGCATTTCGCACTCCTCAGATATCAGAAACTCCGCTCAAAGGATCTATGCTTCCTGCATGAGTGATCGGCCCGTTCGCCGATAACGAtctt                    <  1:3763590/150‑1 (MQ=255)
                                                                                                                                tacctctacctcTTTTTAGGTCAGGATCACCACGCAAGGTTCACTGCTGCTCAATTGCATTTCGCACTCCTCAGATATCAGAAACTCCGCTCAAAGGATCTATGCTTCCTGCATGAGTGATCGGCCCGTTCGCCGATAACGATCttcttt                <  1:549736/150‑1 (MQ=255)
                                                                                                                                tacctctacctcTTTTTAGGTCAGGATCACCACGCAAGGTTCACTGCTGCTCAATTGCATTTCGCACTCCTCAGATATCAGAAACTCCGCTCAAAGGATCTATGCTTCCTGCATGAGTGATCGGCCCGTTCGCCGATAACGATCttcttt                <  1:2135577/150‑1 (MQ=255)
                                                                                                                                  cctctacctctTTTTAGGTCAGGATCACCACGCAAGGTTCACTGCTGCTCAATTGCATTTCGCACTCCTCAGATATCAGAAACTCCGCTCAAAGGATCTATGCTTCCTGCATGAGTGATCGGCCCGTTCGCCGATAACGATCttctttct              <  2:1575892/150‑1 (MQ=255)
                                                                                                                                  cctctacctctTTTTAGGTCAGGATCACCACGCAAGGTTCACTGCTGCTCAATTGCATTTCGCACTCCTCAGATATCAGAAACTCCGCTCAAAGGATCTATGCTTCCTGCATGAGTGATCGGCCCGTTCGCCGATAACGATCttctttct              >  2:1652104/1‑150 (MQ=255)
                                                                                                                                  cctctacctctTTTTAGGTCAGGATCACCACGCAAGGTTCACTGCTGCTCAATTGCATTTCGCACTCCTCAGATATCAGAAACTCCGCTCAAAGGATCTATGCTTCCTGCATGAGTGATCGGCCCGTTCGCCGATAACGATCttctttct              <  2:2961946/150‑1 (MQ=255)
                                                                                                                                   ctctacctctTTTTAGGTCAGGATCACCACGCAAGGTTCACTGCTGCTCAATTGCATTTCGCACTCCTCAGATATCAGAAACTCCGCTCAAAGGATCTATGCTTCCTGCATGAGTGATCGGCCCGTTCGCCGATAACGATCTtctttctt             >  1:3438101/1‑150 (MQ=255)
                                                                                                                                     ctacctctTTTTAGGTCAGGATCACCACGCAAGGTTCACTGCTGCTCAATTGCATTTCGCACTCCTCAGATATCAGAAACTCCGCTCAAAGGATCTATGCTTCCTGCATGAGTGATCGGCCCGTTCGCCGATAACGATCTTCTTTCTTTa           <  1:14269/150‑1 (MQ=255)
                                                                                                                                      tacctctTTTTAGGTCAGGATCACCACGCAAGGTTCACTGCTGCTCAATTGCATTTCGCACTCCTCAGATATCAGAAACTCCGCTCAAAGGATCTATGCTTCCTGCATGAGTGATCGGCCCGTTCGCCGATAACGATCTTCTTTCTTTAg          >  1:594268/1‑150 (MQ=255)
                                                                                                                                        cctctTTTTAGGTCAGGATCACCACGCAAGGTTCACTGCTGCTCAATTGCATTTCGCACTCCTCAGATATCAGAAACTCCGCTCAAAGGATCTATGCTTCCTGCATAAGTGATCGGCCCGTTCGCCGATAACGATCTTCTTTCTTTAGCa        >  1:3384087/1‑150 (MQ=255)
                                                                                                                                         ctctTTTTAGGTCAGGATCACCACGCAAGGTTCACTGCTGCTCAATTGCATTTCGCACTCCTCAGATATCAGAAACTCCGCTCAAAGGATCTATGCTTCCTGCATGAGTGATCGGCCCGTTCGCCGATAACGATCTTCTTTCTTTAGCAc       >  1:2407300/1‑150 (MQ=255)
                                                                                                                                            tttttAGGTCAGGATCACCACGCAAGGTTCACTGCTGCTCAATTGCATTTCGCACTCCTCAGATATCAGAAACTCCGCTCAAAGGATCTATGCTTCCTGCATGAGTGATCGGCCCGTTCGCCGATAACGATCTTCTTTCTTTAGCACGCt    >  2:3672176/1‑150 (MQ=255)
                                                                                                                                            tttttAGGTCAGGATCACCACGCAAGGTTCACTGCTGCTCAATTGCATTTCGCACTCCTCAGATATCAGAAACTCCGCTCAAAGGATCTATGCTTCCTGCATGAGTGATCGGCCCGTTCGCCGATAACGATCTTCTTTCTTTAGCACGCt    >  2:1979485/1‑150 (MQ=255)
                                                                                                                                                 |                                                                                                                                                  
ATGATGTTACGGTTGATGTGGGTGATTAATACGTGATTTAACGTTGAGAGTTCAATGACGTTATTGTTGCACGCGAAGGCGGCCAGGTAGTTTTTCGGAAGGGTAAACGTAGCATGGTTATTGACGCTTACCTCTACCTCTTTTTCGGTCAGGATCACCACGCAAGGTTCACTGCTGCTCAATTGCATTTCGCACTCCTCAGATATCAGAAACTCCGCTCAAAGGATCTATGCTTCCTGCATGAGTGATCGGCCCGTTCGCCGATAACGATCTTCTTTCTTTAGCACGCTTT  >  CP009273/582177‑582468

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
ATGATGTTACGGTTGATGTGGGTGATTAATACGTGATTTAACGTTGAGAGTTCAATGACGTTATTGTTGCACGCGAAGGCGGCCAGGTAGTTTTTCGGAAGGGTAAACGTAGCATGGTTATTGACGCTTACCTCTACCTCTTTTTCGGTCAGGATCACCACGCAAGGTTCACTGCTGCTCAATTGCATTTCGCACTCCTCAGATATCAGAAACTCCGCTCAAAGGATCTATGCTTCCTGCATGAGTGATCGGCCCGTTCGCCGATAACGATCTTCTTTCTTTAGCACGCTTTTTA  >  CP009273/582177‑582471
                                                                                                                                                 |                                                                                                                                                     
ATGATGTTACGGTTGATGTGGGTGATTAATACGTGATTTAACGTTGAGAGTTCAATGACGTTATTGTTGCACGCGAAGGCGGCCAGGTAGTTTTTCGGAAGGGTAAACGTAGCATGGTTATTGACGCTTACCTCTACCTCTTTTTAGGTC                                                                                                                                                   >  SRR19973061.2175171/1‑150 (MQ=60)
  GATGTTACGGTTGATGTGGGTGATTAATACGTGATTTAACGTTGAGAGTTCAATGACGTTATTGTTGCACGCGAAGGCGGCCAGGTAGTTTTTCGGAAGGGTAAACGTAGCATGGTTATTGACGCTTACCTCTACCTCTTTTTAGGTCAG                                                                                                                                                 >  SRR19973061.1947108/1‑150 (MQ=60)
   ATGTTACGGTTGATGTGGGTGATTAATACGTGATTTAACGTTGAGAGTTCAATGACGTTATTGTTGCACGCGAAGGCGGCCAGGTAGTTTTTCGGAAGGGTAAACGTAGCATGGTTATTGACGCTTACCTCTACCTCTTTTTAGGTCAGG                                                                                                                                                >  SRR19973061.3047929/1‑150 (MQ=60)
    TGTTACGGTTGATGTGGGTGATTAATACGTGATTTAACGTTGAGAGTTCAATGACGTTATTGTTGCACGCGAAGGCGGCCAGGTAGTTTTTCGGAAGGGTAAACGTAGCATGGTTATTGACGCTTACCTCTACCTCTTTTTAGGTCAGGA                                                                                                                                               <  SRR19973061.3535716/150‑1 (MQ=60)
        ACGGTTGATGTGGGTGATTAATACGTGATTTAACGTTGAGAGTTCAATGACGTTATTGTTGCACGCGAAGGCGGCCAGGTAGTTTTTCGGAAGGGTAAACGTAGCATGGTTATTGACGCTTACCTCTACCTCTTTTTAGGTCAGGATCAC                                                                                                                                           >  SRR19973061.3338117/1‑150 (MQ=60)
           GTTGATGTGGGTGATTAATACGTGATTTAACGTTGAGAGTTCAATGACGTTATTGTTGCACGCGAAGGCGGCCAGGTAGTTTTTCGGAAGGGTAAACGTAGCATGGTTATTGACGCTTACCTCTACCTCTTTTTAGGTCAGGATCACCAC                                                                                                                                        <  SRR19973061.3518533/150‑1 (MQ=60)
            TTGATGTGGGTGATTAATACGTGATTTAACGTTGAGAGTTCAATGACGTTATTGTTGCACGCGAAGGCGGCCAGGTAGTTTTTCGGAAGGGTAAACGTAGCATGGTTATTGACGCTTACCTCTACCTCTTTTTAGGTCAGGATCACCACG                                                                                                                                       <  SRR19973061.1059294/150‑1 (MQ=60)
              GATGTGGGTGATTAATACGTGATTTAACGTTGAGAGTTCAATGACGTTATTGTTGCACGCGAAGGCGGCCAGGTAGTTTTTCGGAAGGGTAAACGTAGCATGGTTATTGACGCTTACCTCTACCTCTTTTTAGGTCAGGATCACCACGCA                                                                                                                                     <  SRR19973061.2774563/150‑1 (MQ=60)
                   GNGTGATTAATACGTGATTTAACGTTGAGAGTTCAATGACGTTATTGTTGCACGCGAAGGCGGCCAGGTAGTTTTTCGGAAGGGTAAACGTAGCATGGTTATTGACGCTTACCTCTACCTCTTTTTAGGTCAGGATCACCACGCAAGGTT                                                                                                                                >  SRR19973061.2535181/1‑150 (MQ=60)
                   GGGTGATTAATACGTGATTTAACGTTGAGAGTTCAATGACGTTATTGTTGCACGCGAAGGCGGCCAGGTAGTTTTTCGGAAGGGTAAACGTAGCATGGTTATTGACGCTTACCTCTACCTCTTTTTAGGTCAGGATCACCACGCAAGGTT                                                                                                                                >  SRR19973061.424833/1‑150 (MQ=60)
                     GTGATTAATACGTGATTTAACGTTGAGAGTTCAATGACGTTATTGTTGCACGCGAAGGCGGCCAGGTAGTTTTTCGGAAGGGTAAACGTAGCATGGTTATTGACGCTTACCTCTACCTCTTTTTAGGTCAGGATCACCACGCAAGGTTCA                                                                                                                              <  SRR19973061.1356982/150‑1 (MQ=60)
                       GATTAATACGTGATTTAACGTTGAGAGTTCAATGACGTTATTGTTGCACGCGAAGGCGGCCAGGTAGTTTTTCGGAAGGGTAAACGTAGCATGGTTATTGACGCTTACCTCTACCTCTTTTTAGGTCAGGATCACCACGCAAGGTTCACT                                                                                                                            <  SRR19973061.1173405/150‑1 (MQ=60)
                        ATTAATACGTGATTTAACGTTGAGAGTTCAATGACGTTATTGTTGCACGCGAAGGCGGCCAGGTAGTTTTTCGGAAGGGTAAACGTAGCATGGTTATTGACGCTTACCTCTACCTCTTTTTAGGTCAGGATCACCACGCAAGGTTCACTG                                                                                                                           >  SRR19973061.1877176/1‑150 (MQ=60)
                        ATTAATACGTGATTTAACGTTGAGAGTTCAATGACGTTATTGTTGCACGCGAAGGCGGCCAGGTAGTTTTTCGGAAGGGTAAACGTAGCATGGTTATTGACGCTTACCTCTACCTCTTTTTAGGTCAGGATCACCACGCAAGGTTCACTG                                                                                                                           >  SRR19973061.2018111/1‑150 (MQ=60)
                          TAATACGTGATTTAACGTTGAGAGTTCAATGACGTTATTGTTGCACGCGAAGGCGGCCAGGTAGTTTTTCGGAAGGGTAAACGTAGCATGGTTATTGACGCTTACCTCTACCTCTTTTTAGGTCAGGATCACCACGCAAGGTTCACTGCT                                                                                                                         >  SRR19973061.613313/1‑150 (MQ=60)
                           AATACGTGATTTAACGTTGAGAGTTCAATGACGTTATTGTTGCACGCGAAGGCGGCCAGGTAGTTTTTCGGAAGGGTAAACGTAGCATGGTTATTGACGCTTACCTCTACCTCTTTTTAGGTCAGGATCACCACGCAAGGTTCACTGCTG                                                                                                                        <  SRR19973061.3806829/150‑1 (MQ=60)
                            ATACGTGATTTAACGTTGAGAGTTCAATGACGTTATTGTTGCACGCGAAGGCGGCCAGGTAGTTTTTCGGAAGGGTAAACGTAGCATGGTTATTGACGCTTACCTCTACCTCTTTTTAGGTCAGGATCACCACGCAAGGTTCACTGCTGC                                                                                                                       >  SRR19973061.3558225/1‑150 (MQ=60)
                            ATACGTGATTTAACGTTGAGAGTTCAATGACGTTATTGTTGCACGCGAAGGCGGCCAGGTAGTTTTTCGGAAGGGTAAACGTAGCATGGTTATTGACGCTTACCTCTACCTCTTTTTAGGTCAGGATCACCACGCAAGGTTCACTGCTGC                                                                                                                       >  SRR19973061.3780523/1‑150 (MQ=60)
                            ATACGTGATTTAACGTTGAGAGTTCAATGACGTTATTGTTGCACGCGAAGGCGGCCAGGTAGTTTTTCGGAAGGGTAAACGTAGCATGGTTATTGACGCTTACCTCTACCTCTTTTTAGGTCAGGATCACCACGCAAGGTTCACTGCTGC                                                                                                                       >  SRR19973061.3780857/1‑150 (MQ=60)
                            ATACGTGATTTAACGTTGAGAGTTCAATGACGTTATTGTTGCACGCGAAGGCGGCCAGGTAGTTTTTCGGAAGGGTAAACGTAGCATGGTTATTGACGCTTACCTCTACCTCTTTTTAGGTCAGGATCACCACGCAAGGTTCACTGCTGC                                                                                                                       >  SRR19973061.3558222/1‑150 (MQ=60)
                             TACGTGATTTAACGTTGAGAGTTCAATGACGTTATTGTTGCACGCGAAGGCGGCCAGGTAGTTTTTCGGAAGGGTAAACGTAGCATGGTTATTGACGCTTACCTCTACCTCTTTTTAGGTCAGGATCACCACGCAAGGTTCACTGCTGCT                                                                                                                      <  SRR19973061.865354/150‑1 (MQ=60)
                              cagacgtgtgctcttccgatcTCAATGACGTTATTGTTGCACGCGAAGGCGGCCAGGTAGTTTTTCGGAAGGGTAAACGTAGCATGGTTATTGACGCTTACCTCTACCTCTTTTTAGGTCAGGATCACCACGCAAGGTTCACTGCTGCTC                                                                                                                     <  SRR19973061.562516/129‑1 (MQ=60)
                              cagacgtgtgctcttccgatcTCAATGACGTTATTGTTGCACGCGAAGGCGGCCAGGTAGTTTTTCGGAAGGGTAAACGTAGCATGGTTATTGACGCTTACCTCTACCTCTTTTTAGGTCAGGATCACCACGCAAGGTTCACTGCTGCTC                                                                                                                     <  SRR19973061.562512/129‑1 (MQ=60)
                                GTGATTTAACGTTGAGAGTTCAATGACGTTATTGTTGCACGCGAAGGCGGCCAGGTAGTTTTTCGGAAGGGTAAACGTAGCATGGTTATTGACGCTTACCTCTACCTCTTTTTAGGTCAGGATCACCACGCAAGGTTCACTGCTGCTCAA                                                                                                                   <  SRR19973061.1247683/150‑1 (MQ=60)
                                GTGATTTAACGTTGAGAGTTCAATGACGTTATTGTTGCACGCGAAGGCGGCCAGGTAGTTTTTCGGAAGGGTAAACGTAGCATGGTTATTGACGCTTACCTCTACCTCTTTTTAGGTCAGGATCACCACGCAAGGTTCACTGCTGCTCAA                                                                                                                   <  SRR19973061.3432949/150‑1 (MQ=60)
                                       AACGTTGAGAGTTCAATGACGTTATTGTTGCACGCGAAGGCGGCCAGGTAGTTTTTCGGAAGGGTAAACGTAGCATGGTTATTGACGCTTACCTCTACCTCTTTTTAGGTCAGGATCACCACGCAAGGTTCACTGCTGCTCAATTGCATT                                                                                                            <  SRR19973061.774327/150‑1 (MQ=60)
                                       AACGTTGAGAGTTCAATGACGTTATTGTTGCACGCGAAGGCGGCCAGGTAGTTTTTCGGAAGGGTAAACGTAGCATGGTTATTGACGCTTACCTCTACCTCTTTTTAGGTCAGGATCACCACGCAAGGTTCACTGCTGCTCAATTGCATT                                                                                                            <  SRR19973061.1346685/150‑1 (MQ=60)
                                       AACGTTGAGAGTTCAATGACGTTATTGTTGCACGCGAAGGCGGCCAGGTAGTTTTTCGGAAGGGTAAACGTAGCATGGTTATTGACGCTTACCTCTACCTCTTTTTAGGTCAGGATCACCACGCAAGGTTCACTGCTGCTCAATTGCATT                                                                                                            <  SRR19973061.1346007/150‑1 (MQ=60)
                                       AACGTTGAGAGTTCAATGACGTTATTGTTGCACGCGAAGGCGGCCAGGTAGTATTTCGGAAGGGTAAACGTAGCATGGTTATTGACGCTTACCTCTACCTCTTTTTAGGTCAGGATCACCACGCAAGGTTCACTGCTGCTCAATTGCATT                                                                                                            >  SRR19973061.2123426/1‑150 (MQ=60)
                                         CGTTGAGAGTTCAATGACGTTATTGTTGCACGCGAAGGCGGCCAGGTAGTTTTTCGGAAGGGTAAACGTAGCATGGTTATTGACGCTTACCTCTACCTCTTTTTAGGTCAGGATCACCACGCAAGGTTCACTGCTGCTCAATTGCATTTC                                                                                                          <  SRR19973061.744879/150‑1 (MQ=60)
                                         CGTTGAGAGTTCAATGACGTTATTGTTGCACGCGAAGGCGGCCAGGTAGTTTTTCGGAAGGGTAAACGTAGCATGGTTATTGACGCTTACCTCTACCTCTTTTTAGGTCAGGATCACCACGCAAGGTTCACTGCTGCTCAATTGCATTTC                                                                                                          <  SRR19973061.3618235/150‑1 (MQ=60)
                                         CGTTGAGAGTTCAATGACGTTATTGTTGCACGCGAAGGCGGCCAGGTAGTTTTTCGGAAGGGTAAACGTAGCATGGTTATTGACGCTTACCTCTACCTCTTTTTAGGTCAGGATCACCACGCAAGGTTCACTGCTGCTCAATTGCATTTC                                                                                                          >  SRR19973061.3505256/1‑150 (MQ=60)
                                          GTTGAGAGTTCAATGACGTTATTGTTGCACGCGAAGGCGGCCAGGTAGTTTTTCGGAAGGGTAAACGTAGCATGGTTATTGACGCTTACCTCTACCTCTTTTTAGGTCAGGATCACCACGCAAGGTTCACTGCTGCTCAATTGCATTTCG                                                                                                         >  SRR19973061.2266761/1‑150 (MQ=60)
                                           TTGAGAGTTCAATGACGTTATTGTTGCACGCGAAGGCGGCCAGGTAGTTTTTCGGAAGGGTAAACGTAGCATGGTTATTGACGCTTACCTCTACCTCTTTTTAGGTCAGGATCACCACGCAAGGTTCACTGCTGCTCAATTGCATTTCGC                                                                                                        >  SRR19973061.279719/1‑150 (MQ=60)
                                                   TCAATGACGTTATTGTTGCACGCGAAGGCGGCCAGGTAGTTTTTCGGAAGGGTAAACGTAGCATGGTTATTGACGCTTACCTCTACCTCTTTTTAGGTCAGGATCACCACGCAAGGTTCACTGCTGCTCAATTGCATTTCGCACTCCTCA                                                                                                <  SRR19973061.73550/150‑1 (MQ=60)
                                                   TCAATGACGTTATTGTTGCACGCGAAGGCGGCCAGGTAGTTTTTCGGAAGGGTAAACGTAGCATGGTTATTGACGCTTACCTCTACCTCTTTTTAGGTCAGGATCACCACGCAAGGTTCACTGCTGCTCAATTGCATTTCGCACTCCTCA                                                                                                <  SRR19973061.74873/150‑1 (MQ=60)
                                                    CAATGACGTTATTGTTGCACGCGAAGGCGGCCAGGTAGTTTTTCGGAAGGGTAAACGTAGCATGGTTATTGACGCTTACCTCTACCTCTTTTTAGGTCAGGATCACCACGCAAGGTTCACTGCTGCTCAATTGCATTTCGCACTCCTCAG                                                                                               <  SRR19973061.2803772/150‑1 (MQ=60)
                                                      ATGACGTTATTGTTGCACGCGAAGGCGGCCAGGTAGTTTTTCGGAAGGGTAAACGTAGCATGGTTATTGACGCTTACCTCTACCTCTTTTTAGGTCAGGATCACCACGCAAGGTTCACTGCTGCTCAATTGCATTTCGCACTCCTCAGAT                                                                                             >  SRR19973061.1764021/1‑150 (MQ=60)
                                                       TGACGTTATTGTTGCACGCGAAGGCGGCCAGGTAGTTTTTCGGAAGGGTAAACGTAGCATGGTTATTGACGCTTACCTCTACCTCTTTTTAGGTCAGGATCACCACGCAAGGTTCACTGCTGCTCAATTGCATTTCGCACTCCTCAGATA                                                                                            <  SRR19973061.3623370/150‑1 (MQ=60)
                                                        GACGTTATTGTTGCACGCGAAGGCGGCCAGGTAGTTTTTCGGAAGGGTAAACGTAGCATGGTTATTGACGCTTACCTCTACCTCTTTTTAGGTCAGGATCACCACGCAAGGTTCACTGCTGCTCAATTGCATTTCGCACTCCTCAGATAT                                                                                           >  SRR19973061.2322277/1‑150 (MQ=60)
                                                        GACGTTATTGTTGCACGCGAAGGCGGCCAGGTAGTTTTTCGGAAGGGTAAACGTAGCATGGTTATTGACGCTTACCTCTACCTCTTTTTAGGTCAGGATCACCACGCAAGGTTCACTGCTGCTCAATTGCATTTCGCACTCCTCAGATAT                                                                                           >  SRR19973061.411144/1‑150 (MQ=60)
                                                           GTTATTGTTGCACGCGAAGGCGGCCAGGTAGTTTTTCGGAAGGGTAAACGTAGCATGGTTATTGACGCTTACCTCTACCTCTTTTTAGGTCAGGATCACCACGCAAGGTTCACTGCTGCTCAATTGCATTTCGCACTCCTCAGATATCAG                                                                                        >  SRR19973061.2719211/1‑150 (MQ=60)
                                                             TATTGTTGCACGCGAAGGCGGCCAGGTAGTTTTTCGGAAGGGTAAACGTAGCATGGTTATTGACGCTTACCTCTACCTCTTTTTAGGTCAGGATCACCACGCAAGGTTCACTGCTGCTCAATTGCATTTCGCACTCCTCAGATATCAGAA                                                                                      >  SRR19973061.2170034/1‑150 (MQ=60)
                                                             TATTGTTGCACGCGAAGGCGGCCAGGTAGTTTTTCGGAAGGGTAAACGTAGCATGGTTATTGACGCTTACCTCTACCTCTTTTTAGGTCAGGATCACCACGCAAGGTTCACTGCTGCTCAATTGCATTTCGCACTCCTCAGATATCAGAA                                                                                      >  SRR19973061.991689/1‑150 (MQ=60)
                                                              ATTGTTGCACGCGAAGGCGGCCAGGTAGTTTTTCGGAAGGGTAAACGTAGCATGGTTATTGACGCTTACCTCTACCTCTTTTTAGGTCAGGATCACCACGCAAGGTTCACTGCTGCTCAATTGCATTTCGCACTCCTCAGATATCAGAAA                                                                                     >  SRR19973061.3684558/1‑150 (MQ=60)
                                                                       CGCGAAGGCGGCCAGGTAGTTTTTCGGAAGGGTAAACGTAGCATGGTTATTGACGCTTACCTCTACCTCTTTTTAGGTCAGGATCACCACGCAAGGTTCACTGCTGCTCAATTGCATTTCGCACTCCTCAGATATCAGAAACTCCGCTCA                                                                            >  SRR19973061.2273505/1‑150 (MQ=60)
                                                                         CGAAGGCGGCCAGGTAGTTTTTCGGAAGGGTAAACGTAGCATGGTTATTGACGCTTACCTCTACCTCTTTTTAGGTCAGGATCACCACGCAAGGTTCACTGCTGCTCAATTGCATTTCGCACTCCTCAGATATCAGAAACTCCGCTCAAA                                                                          >  SRR19973061.3330126/1‑150 (MQ=60)
                                                                         CGAAGGCGGCCAGGTAGTTTTTCGGAAGGGTAAACGTAGCATGGTTATTGACGCTTACCTCTACCTCTTTTTAGGTCAGGATCACCACGCAAGGTTCACTGCTGCTCAATTGCATTTCGCACTCCTCAGATATCAGAAACTCCGCTCAAA                                                                          >  SRR19973061.2552713/1‑150 (MQ=60)
                                                                          GAAGGCGGCCAGGTAGTTTTTCGGAAGGGTAAACGTAGCATGGTTATTGACGCTTACCTCTACCTCTTTTTAGGTCAGGATCACCACGCAAGGTTCACTGCTGCTCAATTGCATTTCGCACTCCTCAGATATCAGAAACTCCGCTCAAAG                                                                         >  SRR19973061.284432/1‑150 (MQ=60)
                                                                                GGCCAGGTAGTTTTTCGGAAGGGTAAACGTAGCATGGTTATTGACGCTTACCTCTACCTCTTTTTAGGTCAGGATCACCACGCAAGGTTCACTGCTGCTCAATTGCATTTCGCACTCCTCAGATATCAGAAACTCCGCTCAAAGGATCTA                                                                   <  SRR19973061.2021251/150‑1 (MQ=60)
                                                                                GGCCAGGTAGTTTTTCGGAAGGGTAAACGTAGCATGGTTATTGACGCTTACCTCTACCTCTTTTTAGGTCAGGATCACCACGCAAGGTTCACTGCTGCTCAATTGCATTTCGCACTCCTCAGATATCAGAAACTCCGCTCAAAGGATCTA                                                                   >  SRR19973061.2282286/1‑150 (MQ=60)
                                                                                 GCCAGGTAGTTTTTCGGAAGGGTAAACGTAGCATGGTTATTGACGCTTACCTCTACCTCTTTTTAGGTCAGGATCACCACGCAAGGTTCACTGCTGCTCAATTGCATTTCGCACTCCTCAGATATCAGAAACTCCGCTCAAAGGATCTAT                                                                  <  SRR19973061.649748/150‑1 (MQ=60)
                                                                                  CCAGGTAGTTTTTCGGAAGGGTAAACGTAGCATGGTTATTGACGCTTACCTCTACCTCTTTTTAGGTCAGGATCACCACGCAAGGTTCACTGCTGCTCAATTGCATTTCGCACTCCTCAGATATCAGAAACTCCGCTCAAAGGATCTATG                                                                 <  SRR19973061.2657399/150‑1 (MQ=60)
                                                                                   CAGGTAGTTTTTCGGAAGGGTAAACGTAGCATGGTTATTGACGCTTACCTCTACCTCTTTTTAGGTCAGGATCACCACGCAAGGTTCACTGCTGCTCAATTGCATTTCGCACTCCTCAGATATCAGAAACTCCGCTCAAAGGATCTATGC                                                                >  SRR19973061.2969592/1‑150 (MQ=60)
                                                                                     GGTAGTTTTTCGGAAGGGTAAACGTAGCATGGTTATTGACGCTTACCTCTACCTCTTTTTAGGTCAGGATCACCACGCAAGGTTCACTGCTGCTCAATTGCATTTCGCACTCCTCAGATATCAGAAACTCCGCTCAAAGGATCTATGCTT                                                              >  SRR19973061.265272/1‑150 (MQ=60)
                                                                                      GTAGTTTTTCGGAAGGGTAAACGTAGCATGGTTATTGACGCTTACCTCTACCTCTTTTTAGGTCAGGATCACCACGCAAGGTTCACTGCTGCTCAATTGCATTTCGCACTCCTCAGATATCAGAAACTCCGCTCAAAGGATCTATGCTTC                                                             <  SRR19973061.835651/150‑1 (MQ=60)
                                                                                       TAGTTTTTCGGAAGGGTAAACGTAGCATGGTTATTGACGCTTACCTCTACCTCTTTTTAGGTCAGGATCACCACGCAAGGTTCACTGCTGCTCAATTGCATTTCGCACTCCTCAGATATCAGAAACTCCGCTCAAAGGATCTATGCTTCC                                                            >  SRR19973061.619534/1‑150 (MQ=60)
                                                                                       TAGATTTTCGGAAGGGTAAACGTAGCATGGTTATTGACGCTTACCTCTACCTCTTTTTAGGTCAGGATCACCACGCAAGGTTCACTGCTGCTCAATTGCATTTCGCACTCCTCAGATATCAGAAACTCCGCTCAAAGGATCTATGCTTCC                                                            >  SRR19973061.166270/1‑150 (MQ=60)
                                                                                        AGTTTTTCGGAAGGGTAAACGTAGCATGGTTATTGACGCTTACCTCTACCTCTTTTTAGGTCAGGATCACCACGCAAGGTTCACTGCTGCTCAATTGCATTTCGCACTCCTCAGATATCAGAAACTCCGCTCAAAGGATCTATGCTTCCT                                                           >  SRR19973061.1132138/1‑150 (MQ=60)
                                                                                          TTTTTCGGAAGGGTAAACGTAGCATGGTTATTGACGCTTACCTCTACCTCTTTTTAGGTCAGGATCACCACGCAAGGTTCACTGCTGCTCAATTGCATTTCGCACTCCTCAGATATCAGAAACTCCGCTCAAAGGATCTATGCTTCCTGC                                                         <  SRR19973061.3474118/150‑1 (MQ=60)
                                                                                                GGAAGGGTAAACGTAGCATGGTTATTGACGCTTACCTCTACCTCTTTTTAGGTCAGGATCACCACGCAAGGTTCACTGCTGCTCAATTGCATTTCGCACTCCTCAGATATCAGAAACTCCGCTCAAAGGATCTATGCTTCCTGCATGAGT                                                   >  SRR19973061.3108520/1‑150 (MQ=60)
                                                                                                   AGGGTAAACGTAGCATGGTTATTGACGCTTACCTCTACCTCTTTTTAGGTCAGGATCACCACGCAAGGTTCACTGCTGCTCAATTGCATTTCGCACTCCTCAGATATCAGAAACTCCGCTCAAAGGATCTATGCTTCCTGCATGAGTGAT                                                >  SRR19973061.1119827/1‑150 (MQ=60)
                                                                                                    GGGTAAACGTAGCATGGTTATTGACGCTTACCTCTACCTCTTTTTAGGTCAGGATCACCACGCAAGGTTCACTGCTGCTCAATTGCATTTCGCACTCCTCAGATATCAGAAACTCCGCTCAAAGGATCTATGCTTCCTGCATGAGTGATC                                               <  SRR19973061.2309184/150‑1 (MQ=60)
                                                                                                         AACGTAGCATGGTTATTGACGCTTACCTCTACCTCTTTTTAGGTCAGGATCACCACGCAAGGTTCACTGCTGCTCAATTGCATTTCGCACTCCTCAGATATCAGAAACTCCACTCAAAGGATCTATGCTTCCTGCATGAGTGATCGGCCC                                          >  SRR19973061.119311/1‑150 (MQ=60)
                                                                                                             TAGCATGGTTATTGACGCTTACCTCTACCTCTTTTTAGGTCAGGATCACCACGCAAGGTTCACTGCTGCTCAATTGCATTTCGCACTCCTCAGATATCAGAAACTCCGCTCAAAGGATCTATGCTTCCTGCATGAGTGATCGGCCCGTTC                                      >  SRR19973061.2910392/1‑150 (MQ=60)
                                                                                                             TAGCATGGTTATTGACGCTTACCTCTACCTCTTTTTAGGTCAGGATCACCACGCAAGGTTCACTGCTGCTCAATTGCATTTCGCACTCCTCAGATATCAGAAACTCCGCTCAAAGGATCTATGCTTCCTGCATGAGTGATCGGCCCGTTC                                      >  SRR19973061.2452858/1‑150 (MQ=60)
                                                                                                                   GGTTATTGACGCTTACCTCTACCTCTTTTTAGGTCAGGATCACCACGCAAGGTTCACTGCTGCTCAATTGCATTTCGCACTCCTCAGATATCAGAAACTCCGCTCAAAGGATCTATGCTTCCTGCATGAGTGATCGGCCCGTTCGCCGAT                                >  SRR19973061.663669/1‑150 (MQ=60)
                                                                                                                    GTTATTGACGCTTACCTCTACCTCTTTTTAGGTCAGGATCACCACGCAAGGTTCACTGCTGCTCAATTGCATTTCGCACTCCTCAGATATCAGAAACTCCGCTCAAAGGATCTATGCTTCCTGCATGAGTGATCGGCCCGTTCGCCGATA                               >  SRR19973061.3023361/1‑150 (MQ=60)
                                                                                                                      TATTGACGCTTACCTCTACCTCTTTTTAGGTCAGGATCACCACGCAAGGTTCACTGCTGCTCAATTGCATTTCGCACTCCTCAGATATCAGAAACTCCGCTCAAAGGATCTATGCTTCCTGCATGAGTGATCGGCCCGTTCGCCGATAAC                             <  SRR19973061.601894/150‑1 (MQ=60)
                                                                                                                      TATTGACGCTTACCTCTACCTCTTTTTAGGTCAGGATCACCACGCAAGGTTCACTGCTGCTCAATTGCATTTCGCACTCCTCAGATATCAGAAACTCCGCTCAAAGGATCTATGCTTCCTGCATGAGTGATCGGCCCGTTCGCCGATAAC                             >  SRR19973061.1614662/1‑150 (MQ=60)
                                                                                                                         TGACGCTTACCTCTACCTCTTTTTAGGTCAGGATCACCACGCAAGGTTCACTGCTGCTCAATTGCATTTCGCACTCCTCAGATATCAGAAACTCCGCTCAAAGGATCTATGCTTCCTGCATGAGTGATCGGCCCGTTCGCCGATAACGAT                          >  SRR19973061.3182501/1‑150 (MQ=60)
                                                                                                                         TGACGCTTACCTCTACCTCTTTTTAGGTCAGGATCACCACGCAAGGTTCACTGCTGCTCAATTGCATTTCGCACTCCTCAGATATCAGAAACTCCGCTCAAAGGATCTATGCTTCCTGCATGAGTGATCGGCCCGTTCGCCGATAACGAT                          >  SRR19973061.898337/1‑150 (MQ=60)
                                                                                                                         TGACGCTTACCTCTACCTCTTTTTAGGTCAGGATCACCACGCAAGGTTCACTGCTGCTCAATTGCATTTCGCACTCCTCAGATATCAGAAACTCCGCTCAAAGGATCTATGCTTCCTGCATGAGTGATCGGCCCGTTCGCCGATAACGAT                          <  SRR19973061.452103/150‑1 (MQ=60)
                                                                                                                          GACGCTTACCTCTACCTCTTTTTAGGTCAGGATCACCACGCAAGGTTCACTGCTGCTCAATTGCATTTCGCACTCCTCAGATATCAGAAACTCCGCTCAAAGGATCTATGCTTCCTGCATGAGTGATCGGCCCGTTCGCCGATAACGATC                         >  SRR19973061.1591769/1‑150 (MQ=60)
                                                                                                                            CGCTTACCTCTACCTCTTTTTAGGTCAGGATCACCACGCAAGGTTCACTGCTGCTCAATTGCATTTCGCACTCCTCAGATATCAGAAACTCCGCTCAAAGGATCTATGCTTCCTGCATGAGTGATCGGCCCGTTCGCCGATAACGATCTT                       <  SRR19973061.3773209/150‑1 (MQ=60)
                                                                                                                              CTTACCTCTACCTCTTTTTAGGTCAGGATCACCACGCAAGGTTCACTGCTGCTCAATTGCATTTCGCACTCCTCAGATATCAGAAACTCCGCTCAAAGGATCTATGCTTCCTGCATGAGTGATCGGCCCGTTCGCCGATAACGATCTTCT                     >  SRR19973061.3456854/1‑150 (MQ=60)
                                                                                                                                TACCTCTACCTCTTTTTAGGTCAGGATCACCACGCAAGGTTCACTGCTGCTCAATTGCATTTCGCACTCCTCAGATATCAGAAACTCCGCTCAAAGGATCTATGCTTCCTGCATGAGTGATCGGCCCGTTCGCCGATAACGATCTTCTTT                   <  SRR19973061.551185/150‑1 (MQ=60)
                                                                                                                                TACCTCTACCTCTTTTTAGGTCAGGATCACCACGCAAGGTTCACTGCTGCTCAATTGCATTTCGCACTCCTCAGATATCAGAAACTCCGCTCAAAGGATCTATGCTTCCTGCATGAGTGATCGGCCCGTTCGCCGATAACGATCTTCTTT                   <  SRR19973061.2140940/150‑1 (MQ=60)
                                                                                                                                  CCTCTACCTCTTTTTAGGTCAGGATCACCACGCAAGGTTCACTGCTGCTCAATTGCATTTCGCACTCCTCAGATATCAGAAACTCCGCTCAAAGGATCTATGCTTCCTGCATGAGTGATCGGCCCGTTCGCCGATAACGATCTTCTTTCT                 <  SRR19973061.3185900/150‑1 (MQ=60)
                                                                                                                                   CTCTACCTCTTTTTAGGTCAGGATCACCACGCAAGGTTCACTGCTGCTCAATTGCATTTCGCACTCCTCAGATATCAGAAACTCCGCTCAAAGGATCTATGCTTCCTGCATGAGTGATCGGCCCGTTCGCCGATAACGATCTTCTTTCTT                <  SRR19973061.856326/150‑1 (MQ=60)
                                                                                                                                   CTCTACCTCTTTTTAGGTCAGGATCACCACGCAAGGTTCACTGCTGCTCAATTGCATTTCGCACTCCTCAGATATCAGAAACTCCGCTCAAAGGATCTATGCTTCCTGCATGAGTGATCGGCCCGTTCGCCGATAACGATCTTCTTTCTT                >  SRR19973061.3446918/1‑150 (MQ=60)
                                                                                                                                   CTCTACCTCTTTTTAGGTCAGGATCACCACGCAAGGTTCACTGCTGCTCAATTGCATTTCGCACTCCTCAGATATCAGAAACTCCGCTCAAAGGATCTATGCTTCCTGCATGAGTGATCGGCCCGTTCGCCGATAACGATCTTCTTTCTT                <  SRR19973061.540301/150‑1 (MQ=60)
                                                                                                                                     CTACCTCTTTTTAGGTCAGGATCACCACGCAAGGTTCACTGCTGCTCAATTGCATTTCGCACTCCTCAGATATCAGAAACTCCGCTCAAAGGATCTATGCTTCCTGCATGAGTGATCGGCCCGTTCGCCGATAACGATCTTCTTTCTTTA              <  SRR19973061.14328/150‑1 (MQ=60)
                                                                                                                                      TACCTCTTTTTAGGTCAGGATCACCACGCAAGGTTCACTGCTGCTCAATTGCATTTCGCACTCCTCAGATATCAGAAACTCCGCTCAAAGGATCTATGCTTCCTGCATGAGTGATCGGCCCGTTCGCCGATAACGATCTTCTTTCTTTAG             >  SRR19973061.595818/1‑150 (MQ=60)
                                                                                                                                        CCTCTTTTTAGGTCAGGATCACCACGCAAGGTTCACTGCTGCTCAATTGCATTTCGCACTCCTCAGATATCAGAAACTCCGCTCAAAGGATCTATGCTTCCTGCATAAGTGATCGGCCCGTTCGCCGATAACGATCTTCTTTCTTTAGCA           >  SRR19973061.3392780/1‑150 (MQ=60)
                                                                                                                                         CTCTTTTTAGGTCAGGATCACCACGCAAGGTTCACTGCTGCTCAATTGCATTTCGCACTCCTCAGATATCAGAAACTCCGCTCAAAGGATCTATGCTTCCTGCATGAGTGATCGGCCCGTTCGCCGATAACGATCTTCTTTCTTTAGCAC          >  SRR19973061.2413449/1‑150 (MQ=60)
                                                                                                                                           CTTTTTAGGTCAGGATCACCACGCAAGGTTCACTGCTGCTCAATTGCATTTCGCACTCCTCAGATATCAGAAACTCCGCTCAAAGGATCTATGCTTCCTGCATGAGTGATCGGCCCGTTCGCCGATAACGATCTTCTTTCTTTAGCACGC        >  SRR19973061.215585/1‑150 (MQ=60)
                                                                                                                                           CTTTTTAGGTCAGGATCACCACGCAAGGTTCACTGCTGCTCAATTGCATTTCGCACTCCTCAGATATCAGAAACTCCGCTCAAAGGATCTATGCTTCCTGCATGAGTGATCGGCCCGTTCGCCGATAACGATCTTCTTTCTTTAGCACGC        >  SRR19973061.2130045/1‑150 (MQ=60)
                                                                                                                                            TTTTTAGGTCAGGATCACCACGCAAGGTTCACTGCTGCTCAATTGCATTTCGCACTCCTCAGATATCAGAAACTCCGCTCAAAGGATCTATGCTTCCTGCATGAGTGATCGGCCCGTTCGCCGATAACGATCTTCTTTCTTTAGCACGCT       <  SRR19973061.3163434/150‑1 (MQ=60)
                                                                                                                                            TTTTTAGGTCAGGATCACCACGCAAGGTTCACTGCTGCTCAATTGCATTTCGCACTCCTCAGATATCAGAAACTCCGCTCAAAGGATCTATGCTTCCTGCATGAGTGATCGGCCCGTTCGCCGATAACGATCTTCTTTCTTTAGCACGCT       <  SRR19973061.944623/150‑1 (MQ=60)
                                                                                                                                                 AGGTCAGGATCACCACGCAAGGTTCACTGCTGCTCAATTGCATTTCGCACTCCTCAGATATCAGAAACTCCGCTCAAAGGATCTATGCTTCCTGCATGAGTGATCGGCCCGTTCGCCGATAACGATCTTCTTTCTTTAGCACGCTTTTTA  >  SRR19973061.1352056/1‑150 (MQ=60)
                                                                                                                                                 AGGTCAGGATCACCACGCAAGGTTCACTGCTGCTCAATTGCATTTCGCACTCCTCAGATATCAGAAACTCCGCTCAAAGGATCTATGCTTCCTGCATGAGTGATCGGCCCGTTCGCCGATAACGATCTTCTTTCTTTAGCACGCTTTTTA  >  SRR19973061.273303/1‑150 (MQ=60)
                                                                                                                                                 |                                                                                                                                                     
ATGATGTTACGGTTGATGTGGGTGATTAATACGTGATTTAACGTTGAGAGTTCAATGACGTTATTGTTGCACGCGAAGGCGGCCAGGTAGTTTTTCGGAAGGGTAAACGTAGCATGGTTATTGACGCTTACCTCTACCTCTTTTTCGGTCAGGATCACCACGCAAGGTTCACTGCTGCTCAATTGCATTTCGCACTCCTCAGATATCAGAAACTCCGCTCAAAGGATCTATGCTTCCTGCATGAGTGATCGGCCCGTTCGCCGATAACGATCTTCTTTCTTTAGCACGCTTTTTA  >  CP009273/582177‑582471

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑