Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F534 I1 R1 1235 230.2 7611948 95.1% 7238962 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009273 1,870,743 C→A H272N (CAT→AAT)  yeaN → putative transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,870,7430CA99.5% 607.5 / NA 186H272N (CAT→AAT) yeaNputative transporter
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base A (99/86);  total (100/86)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 190 total aligned reads displayed.

AACTCACTGGTCTATTACGTGATTATTGGCTGGCTTCCGGCGATCCTCATCAGTCACGGCTATAGCGAAGCACAGGCGGGTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATCATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTGGACTCTGCTTTTCGGTTTTGGTTCCGGCGCAACAATGATACTGGGGTTG  >  CP009273/1870596‑1870889
                                                                                                                                                   |                                                                                                                                                  
 aCTCACTGGTCTATTACGTGATTATTGGCTGGCTTCCGGCGATCCTCATCAGTCACGGCTATAGCGAAGCACAGGCGGGTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAAtg                                                                                                                                                 <  1:2441856/150‑1 (MQ=255)
  cTCACTGGTCTATTACGTGATTATTGGCTGGCTTCCGGCGATCCTCATCAGTCACGGCTATAGCGAAGCACAGGCGGGTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAAtgt                                                                                                                                                <  1:577471/150‑1 (MQ=255)
    cACTGGTCTATTACGTGATTATTGGCTGGCTTCCGGCGATCCTCATCAGTCACGGCTATAGCGAAGCACAGGCGGGTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGa                                                                                                                                              >  2:1421325/1‑150 (MQ=255)
    cACTGGTCTATTACGTGATTATTGGCTGGCTTCCGGCGATCCTCATCAGTCACGGCTATAGCGAAGCACAGGCGGGTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGa                                                                                                                                              <  1:2376425/150‑1 (MQ=255)
       tGGTCTATTACGTGATTATTGGCTGGCTTCCGGCGATCCTCATCAGTCACGGCTATAGCGAAGCACAGGCGGGTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAg                                                                                                                                           <  1:763542/150‑1 (MQ=255)
        ggTCTATTACGTGATTATTGGCTGGCTTCCGGCGATCCTCATCAGTCACGGCTATAGCGAAGCACAGGCGGGTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGa                                                                                                                                          >  2:2278342/1‑150 (MQ=255)
            tATTACGTGATTATTGGCTGGCTTCCGGCGATCCTCATCAGTCACGGCTATAGCGAAGCACAGGCGGGTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAg                                                                                                                                      <  1:3533295/150‑1 (MQ=255)
            tATTACGTGATTATTGGCTGGCTTCCGGCGATCCTCATCAGTCACGGCTATAGCGAAGCACAGGCGGGTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAg                                                                                                                                      >  1:1430997/1‑150 (MQ=255)
             aTTACGTGATTATTGGCTGGCTTCCGGCGATCCTCATCAGTCACGGCTATAGCGAAGCACAGGCGGGTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGc                                                                                                                                     >  1:1278227/1‑150 (MQ=255)
              ttACGTGATTATTGGCTGGCTTCCGGCGATCCTCATCAGTCACGGCTATAGCGAAGCACAGGCGGGTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCg                                                                                                                                    <  1:2948074/150‑1 (MQ=255)
               tACGTGATTATTGGCTGGCTTCCGGCGATCCTCATCAGTCACGGCTATAGCGAAGCACAGGCGGGTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGt                                                                                                                                   >  1:1084103/1‑150 (MQ=255)
                 cGTGATTATTGGCTGGCTTCCGGCGATCCTCATCAGTCACGGCTATAGCGAAGCACAGGCGGGTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTgg                                                                                                                                 >  2:1855603/1‑150 (MQ=255)
                    gATTATTGGCTGGCTTCCGGCGATCCTCATCAGTCACGGCTATAGCGAAGCACAGGCGGGTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTAt                                                                                                                              >  1:679505/1‑150 (MQ=255)
                     attattGGCTGGCTTCCGGCGATCCTCATCAGTCACGGCTATAGCGAAGCACAGGCGGGTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTAtt                                                                                                                             >  1:639596/1‑150 (MQ=255)
                      ttattGGCTGGCTTCCGGCGATCCTCATCAGTCACGGCTATAGCGAAGCACAGGCGGGTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTg                                                                                                                            <  1:219669/150‑1 (MQ=255)
                      ttattGGCTGGCTTCCGGCGATCCTCATCAGTCACGGCTATAGCGAAGCACAGGCGGGTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTg                                                                                                                            <  2:413348/150‑1 (MQ=255)
                         ttGGCTGGCTTCCGGCGATCCTCATCAGTCACGGCTATAGCGAAGCACAGGCGGGTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAg                                                                                                                         <  1:3749860/150‑1 (MQ=255)
                          tggctggcTTCCGGCGATCCTCATCAGTCACGGCTATAGCGAAGCACAGGCGGGTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGc                                                                                                                        >  2:924243/1‑150 (MQ=255)
                          tggctggcTTCCGGCGATCCTCATCAGTCACGGCTATAGCGAAGCACAGGCGGGTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGc                                                                                                                        >  1:1051313/1‑150 (MQ=255)
                             ctggctTCCGGCGATCCTCATCAGTCACGGCTATAGCGAAGCACAGGCGGGTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGcgtt                                                                                                                     <  1:638572/150‑1 (MQ=255)
                              tggctTCCGGCGATCCTCATCAGTCACGGCTATAGCGAAGCACAGGCGGGTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGcgttc                                                                                                                    <  1:3166668/150‑1 (MQ=255)
                                  ttCCGGCGATCCTCATCAGTCACGGCTATAGCGAAGCACAGGCGGGTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTg                                                                                                                <  2:1444379/150‑1 (MQ=255)
                                  ttCCGGCGATCCTCATCAGTCACGGCTATAGCGAAGCACAGGCGGGTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTg                                                                                                                >  1:752430/1‑150 (MQ=255)
                                   tCCGGCGATCCTCATCAGTCACGGCTATAGCGAAGCACAGGCGGGTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGc                                                                                                               >  2:413864/1‑150 (MQ=255)
                                     cGGCGATCCTCATCAGTCACGGCTATAGCGAAGCACAGGCGGGTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCt                                                                                                             >  2:334098/1‑150 (MQ=255)
                                      ggCGATCCTCATCAGTCACGGCTATAGCGAAGCACAGGCGGGTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCtt                                                                                                            <  1:2549416/150‑1 (MQ=255)
                                      ggCGATCCTCATCAGTCACGGCTATAGCGAAGCACAGGCGGGTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCtt                                                                                                            <  2:2233167/150‑1 (MQ=255)
                                         gATCCTCATCAGTCACGGCTAGAGCGAAGCACATGCGGGTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGAt                                                                                                         <  2:2041274/150‑1 (MQ=255)
                                           tCCTCATCAGTCACGGCTATAGCGAAGCACAGGCGGGTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGAtgt                                                                                                       <  2:1334282/150‑1 (MQ=255)
                                              tcatcaGTCACGGCTATAGCGAAGCACAGGCGGGTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTgcg                                                                                                    <  2:2904750/150‑1 (MQ=255)
                                                     tCACGGCTATAGCGAAGCACAGGCGGGTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTggg                                                                                             >  1:3739110/1‑150 (MQ=255)
                                                      cACGGCTATAGCGAAGCACAGGCGGGTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGgc                                                                                            >  1:3105921/1‑150 (MQ=255)
                                                      cACGGCTATAGCGAAGCACAGGCGGGTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGgc                                                                                            >  2:3401862/1‑150 (MQ=255)
                                                       aCGGCTATAGCGAAGCACAGGCGGGTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGgcg                                                                                           <  2:1054212/150‑1 (MQ=255)
                                                       aCGGCTATAGCGAAGCACAGGCGGGTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGgcg                                                                                           >  1:1578984/1‑150 (MQ=255)
                                                       aCGGCTATAGCGAAGCACAGGCGGGTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGgcg                                                                                           <  1:1966804/150‑1 (MQ=255)
                                                        cGGCTATAGCGAAGCACAGGCGGGTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGgcgc                                                                                          >  1:2489933/1‑150 (MQ=255)
                                                          gcaataGCGAACCACAGGCGGGATCACTGCCTGGTTAGCTGCAACAAGCCACAGCAGCACCCGGTTTCCTGATCCCACTATACTAACATCATGTCAAAGAACACCGTGGGCTTGCAGCGGTCGTTGCCTTGAGGTGCGCAGTGGGCGCgg                                                                                        >  2:1823313/4‑150 (MQ=255)
                                                            tataGCGAAGCACAGGCGGGTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGtt                                                                                      >  2:3523448/1‑150 (MQ=255)
                                                            tataGCGAAGCACAGGCGGGTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGtt                                                                                      <  1:2532313/150‑1 (MQ=255)
                                                             ataGCGAAGCACAGGCGGGTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTg                                                                                     >  1:1863036/1‑150 (MQ=255)
                                                              taGCGAAGCACAGGCGGGTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTgg                                                                                    >  2:1165546/1‑150 (MQ=255)
                                                                  gAAGCACAGGCGGGTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGctc                                                                                >  2:37175/1‑150 (MQ=255)
                                                                      cacaGGCGGGTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCt                                                                            >  2:3227316/1‑150 (MQ=255)
                                                                         aGGCGGGTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTa                                                                         >  1:3538934/1‑150 (MQ=255)
                                                                         aGGCGGGTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTa                                                                         >  2:3614516/1‑150 (MQ=255)
                                                                          ggCGGGGTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTAt                                                                        <  1:3322203/150‑1 (MQ=255)
                                                                              ggTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCa                                                                    <  1:1781558/150‑1 (MQ=255)
                                                                               gTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAg                                                                   <  1:3236638/150‑1 (MQ=255)
                                                                                ttCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAgc                                                                  <  1:3425686/150‑1 (MQ=255)
                                                                                   aCTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCGGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCa                                                               >  1:1344995/1‑150 (MQ=255)
                                                                                       cATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCAcgcg                                                           >  2:1152581/1‑150 (MQ=255)
                                                                                           gTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCa                                                       <  2:801299/150‑1 (MQ=255)
                                                                                             ttGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCAcc                                                     >  2:1282112/1‑150 (MQ=255)
                                                                                             ttGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCAcc                                                     >  1:214036/1‑150 (MQ=255)
                                                                                              tgctgcAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCt                                                    <  2:1259027/150‑1 (MQ=255)
                                                                                              tgctgcAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCt                                                    <  1:766083/150‑1 (MQ=255)
                                                                                              tgctgcAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCt                                                    >  2:1433670/1‑150 (MQ=255)
                                                                                                ctgcAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTgg                                                  >  2:2191305/1‑150 (MQ=255)
                                                                                                ctgcAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTgg                                                  >  2:484759/1‑150 (MQ=255)
                                                                                                ctgcAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTgg                                                  >  2:691197/1‑150 (MQ=255)
                                                                                                 tgcAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTGGa                                                 >  2:742562/1‑150 (MQ=255)
                                                                                                  gcAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTGGAc                                                >  1:1896323/1‑150 (MQ=255)
                                                                                                  gcAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTGGAc                                                >  1:3332130/1‑150 (MQ=255)
                                                                                                   cAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTGGAct                                               <  2:168096/150‑1 (MQ=255)
                                                                                                        aGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTGGACTCTGCt                                          <  1:1434426/150‑1 (MQ=255)
                                                                                                        aGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTGGACTCTGCt                                          >  2:1167325/1‑150 (MQ=255)
                                                                                                            acaGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTGGACTCTGCTTTTc                                      <  2:543660/150‑1 (MQ=255)
                                                                                                            acaGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTGGACTCTGCTTTTc                                      <  2:1578984/150‑1 (MQ=255)
                                                                                                            acaGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTGGACTCTGCTTTTc                                      <  2:2559662/150‑1 (MQ=255)
                                                                                                             cagcagCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTGGACTCTGCTTTTCg                                     >  2:2265298/1‑150 (MQ=255)
                                                                                                              agcagcACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTGGACTCTGCTTTTCgg                                    >  2:2929321/1‑150 (MQ=255)
                                                                                                              agcagcACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTGGACTCTGCTTTTCgg                                    >  2:1028464/1‑150 (MQ=255)
                                                                                                                cagcaCCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTGGACTCTGCTTTTCGGtt                                  <  1:1330208/150‑1 (MQ=255)
                                                                                                                 agcaCCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTGGACTCTGCTTTTCGGttt                                 <  2:3760471/150‑1 (MQ=255)
                                                                                                                    aCCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTGGACTCTGCTTTTCGGTTTTgg                              >  2:2988561/1‑150 (MQ=255)
                                                                                                                     cccGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTGGACTCTGCTTTTCGGTTTTGGt                             >  1:855186/1‑150 (MQ=255)
                                                                                                                     cccGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTGGACTCTGCTTTTCGGTTTTGGt                             >  1:2597086/1‑150 (MQ=255)
                                                                                                                      ccGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTGGACTCTGCTTTTCGGTTTTGGtt                            <  2:1084103/150‑1 (MQ=255)
                                                                                                                       cGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTGGACTCTGCTTTTCGGTTTTGGTTc                           <  1:2787433/150‑1 (MQ=255)
                                                                                                                       cGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTGGACTCTGCTTTTCGGTTTTGGTTc                           >  1:3018000/1‑150 (MQ=255)
                                                                                                                         gTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTGGACTCTGCTTTTCGGTTTTGGTTCCg                         <  2:887486/150‑1 (MQ=255)
                                                                                                                          tttGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTGGACTCTGCTTTTCGGTTTTGGTTCCgg                        >  2:2870820/1‑150 (MQ=255)
                                                                                                                           ttGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTGGACTCTGCTTTTCGGTTTTGGTTCCGgc                       <  2:2205010/150‑1 (MQ=255)
                                                                                                                             gCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTGGACTCTGCTTTTCGGTTTTGGTTCCGgcgc                     >  2:989933/1‑150 (MQ=255)
                                                                                                                              cTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTGGACTCTGCTTTTCGGTTTTGGTTCCGGCGca                    >  2:2236328/1‑150 (MQ=255)
                                                                                                                                gATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTGGACTCTGCTTTTCGGTTTTGGTTCCGGCGcaac                  <  1:924243/150‑1 (MQ=255)
                                                                                                                                 aTCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCGGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTGGACTCTGCTTTTCGGTTTTGGTTCCGGCGcaaca                 >  1:3263144/1‑150 (MQ=255)
                                                                                                                                   cccACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTGGACTCTGCTTTTCGGTTTTGGTTCCGGCGCAACAAt               <  2:1736118/150‑1 (MQ=255)
                                                                                                                                   cccACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATGGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCTCACGCGATCACCTGGACTCTGCTTTTCGGTTTTGGTTCCGGCGCAACAAt               <  1:2709037/150‑1 (MQ=255)
                                                                                                                                      aCTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCCGCGCACGCGATCACCTGGACTCTGCTTTTCGGTTTTGGTTCCGGCGCAACAATGAt            >  2:2122898/1‑150 (MQ=255)
                                                                                                                                      aCTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTGGACTCTGCTTTTCGGTTTTGGTTCCGGCGCAACAATGAt            >  2:2788188/1‑150 (MQ=255)
                                                                                                                                       cTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTGGACTCTGCTTTTCGGTTTTGGTTCCGGCGCAACAATGATa           <  2:2656956/150‑1 (MQ=255)
                                                                                                                                       cTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTGGACTCTGCTTTTCGGTTTTGGTTCCGGCGCAACAATGATa           <  2:752430/150‑1 (MQ=255)
                                                                                                                                       cTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTGGACTCTGCTTTTCGGTTTTGGTTCCGGCGCAACAATGATa           <  1:704959/150‑1 (MQ=255)
                                                                                                                                       cTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGATGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTGGACTCTGCTTTTCGGTTTTGGTTCCGGCGCAACAATGATa           >  1:999065/1‑150 (MQ=255)
                                                                                                                                        ttttCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTGGACTCTGCTTTTCGGTTTTGGTTCCGGCGCAACAATGATaa          <  2:1430997/150‑2 (MQ=255)
                                                                                                                                          ttCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTGGACTCTGCTTTTCGGTTTTGGTTCCGGCGCAACAATGATACTg        <  1:413864/150‑1 (MQ=255)
                                                                                                                                               aCATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTGGACTCTGCTTTTCGGTTTTGGTTCCGGCGCAACAATGATACTGGGGtt   <  1:2278311/150‑1 (MQ=255)
                                                                                                                                                cataatGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTGGACTCTGCTTTTCGGTTTTGGTTCCGGCGCAACAATGATACTGGGGTTg  <  1:1657345/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
AACTCACTGGTCTATTACGTGATTATTGGCTGGCTTCCGGCGATCCTCATCAGTCACGGCTATAGCGAAGCACAGGCGGGTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATCATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTGGACTCTGCTTTTCGGTTTTGGTTCCGGCGCAACAATGATACTGGGGTTG  >  CP009273/1870596‑1870889

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TCAACTCACTGGTCTATTACGTGATTATTGGCTGGCTTCCGGCGATCCTCATCAGTCACGGCTATAGCGAAGCACAGGCGGGTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATCATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTGGACTCTGCTTTTCGGTTTTGGTTCCGGCGCAACAATGATACTGGGGTTGAC  >  CP009273/1870594‑1870891
                                                                                                                                                     |                                                                                                                                                    
TCAACTCACTGGTCTATTACGTGATTATTGGCTGGCTTCCGGCGATCCTCATCAGTCACGGCTATAGCGAAGCACAGGCGGGTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATA                                                                                                                                                      <  SRR19973061.3581169/150‑1 (MQ=60)
  AACTCACTGGTCTATTACGTGATTATTGGCTGGCTTCCGGCGATCCTCATCAGTCACGGCTATAGCGAAGCACAGGCGGGTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAAT                                                                                                                                                    >  SRR19973061.1334744/1‑150 (MQ=60)
   ACTCACTGGTCTATTACGTGATTATTGGCTGGCTTCCGGCGATCCTCATCAGTCACGGCTATAGCGAAGCACAGGCGGGTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATG                                                                                                                                                   <  SRR19973061.2448081/150‑1 (MQ=60)
    CTCACTGGTCTATTACGTGATTATTGGCTGGCTTCCGGCGATCCTCATCAGTCACGGCTATAGCGAAGCACAGGCGGGTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGT                                                                                                                                                  <  SRR19973061.578976/150‑1 (MQ=60)
      CACTGGTCTATTACGTGATTATTGGCTGGCTTCCGGCGATCCTCATCAGTCACGGCTATAGCGAAGCACAGGCGGGTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGA                                                                                                                                                <  SRR19973061.2382476/150‑1 (MQ=60)
      CACTGGTCTATTACGTGATTATTGGCTGGCTTCCGGCGATCCTCATCAGTCACGGCTATAGCGAAGCACAGGCGGGTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGA                                                                                                                                                <  SRR19973061.254957/150‑1 (MQ=60)
        CTGGTCTATTACGTGATTATTGGCTGGCTTCCGGCGATCCTCATCAGTCACGGCTATAGCGAAGCACAGGCGGGTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAA                                                                                                                                              >  SRR19973061.2495917/1‑150 (MQ=60)
        CTGGTCTATTACGTGATTATTGGCTGGCTTCCGGCGATCCTCATCAGTCACGGCTATAGCGAAGCACAGGCGGGTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAA                                                                                                                                              >  SRR19973061.586041/1‑150 (MQ=60)
         TGGTCTATTACGTGATTATTGGCTGGCTTCCGGCGATCCTCATCAGTCACGGCTATAGCGAAGCACAGGCGGGTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAG                                                                                                                                             <  SRR19973061.765546/150‑1 (MQ=60)
          GGTCTATTACGTGATTATTGGCTGGCTTCCGGCGATCCTCATCAGTCACGGCTATAGCGAAGCACAGGCGGGTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGA                                                                                                                                            <  SRR19973061.2959574/150‑1 (MQ=60)
          GGTCTATTACGTGATTATTGGCTGGCTTCCGGCGATCCTCATCAGTCACGGCTATAGCGAAGCACAGGCGGGTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGA                                                                                                                                            >  SRR19973061.36605/1‑150 (MQ=60)
          GGTCTATTACGTGATTATTGGCTGGCTTCCGGCGATCCTCATCAGTCACGGCTATAGCGAAGCACAGGCGGGTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGA                                                                                                                                            <  SRR19973061.3545969/150‑1 (MQ=60)
             CTATTACGTGATTATTGGCTGGCTTCCGGCGATCCTCATCAGTCACGGCTATAGCGAAGCACAGGCGGGTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCA                                                                                                                                         <  SRR19973061.3274749/150‑1 (MQ=60)
              TATTACGTGATTATTGGCTGGCTTCCGGCGATCCTCATCAGTCACGGCTATAGCGAAGCACAGGCGGGTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAG                                                                                                                                        <  SRR19973061.3542342/150‑1 (MQ=60)
              TATTACGTGATTATTGGCTGGCTTCCGGCGATCCTCATCAGTCACGGCTATAGCGAAGCACAGGCGGGTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAG                                                                                                                                        >  SRR19973061.1434599/1‑150 (MQ=60)
              TATTACGTGATTATTGGCTGGCTTCCGGCGATCCTCATCAGTCACGGCTATAGCGAAGCACAGGCGGGTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAG                                                                                                                                        >  SRR19973061.1434802/1‑150 (MQ=60)
              TATTACGTGATTATTGGCTGGCTTCCGGCGATCCTCATCAGTCACGGCTATAGCGAAGCACAGGCGGGTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACACGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAG                                                                                                                                        <  SRR19973061.3542341/150‑1 (MQ=60)
               ATTACGTGATTATTGGCTGGCTTCCGGCGATCCTCATCAGTCACGGCTATAGCGAAGCACAGGCGGGTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGC                                                                                                                                       >  SRR19973061.1281381/1‑150 (MQ=60)
               ATTACGTGATTATTGGCTGGCTTCCGGCGATCCTCATCAGTCACGGCTATAGCGAAGCACAGGCGGGTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGC                                                                                                                                       >  SRR19973061.1281343/1‑150 (MQ=60)
                TTACGTGATTATTGGCTGGCTTCCGGCGATCCTCATCAGTCACGGCTATAGCGAAGCACAGGCGGGTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCG                                                                                                                                      <  SRR19973061.2955667/150‑1 (MQ=60)
                 TACGTGATTATTGGCTGGCTTCCGGCGATCCTCATCAGTCACGGCTATAGCGAAGCACAGGCGGGTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGT                                                                                                                                     >  SRR19973061.1086882/1‑150 (MQ=60)
                      GATTATTGGCTGGCTTCCGGCGATCCTCATCAGTCACGGCTATAGCGAAGCACAGGCGGGTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTAT                                                                                                                                >  SRR19973061.681265/1‑150 (MQ=60)
                      GATTATTGGCTGGCTTCCGGCGATCCTCATCAGTCACGGCTATAGCGAAGCACAGGCGGGTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTAT                                                                                                                                >  SRR19973061.681351/1‑150 (MQ=60)
                      GATTATTGGCTGGCTTCCGGCGATCCTCATCAGTCACGGCTATAGCGAAGCACAGGCGGGTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTAT                                                                                                                                >  SRR19973061.2210550/1‑150 (MQ=60)
                       ATTATTGGCTGGCTTCCGGCGATCCTCATCAGTCACGGCTATAGCGAAGCACAGGCGGGTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATT                                                                                                                               >  SRR19973061.641222/1‑150 (MQ=60)
                        TTATTGGCTGGCTTCCGGCGATCCTCATCAGTCACGGCTATAGCGAAGCACAGGCGGGTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTG                                                                                                                              <  SRR19973061.145217/150‑1 (MQ=60)
                        TTATTGGCTGGCTTCCGGCGATCCTCATCAGTCACGGCTATAGCGAAGCACAGGCGGGTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTG                                                                                                                              <  SRR19973061.499198/150‑1 (MQ=60)
                        TTATTGGCTGGCTTCCGGCGATCCTCATCAGTCACGGCTATAGCGAAGCACAGGCGGGTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTG                                                                                                                              <  SRR19973061.2060958/150‑1 (MQ=60)
                        TTATTGGCTGGCTTCCGGCGATCCTCATCAGTCACGGCTATAGCGAAGCACAGGCGGGTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTG                                                                                                                              <  SRR19973061.220315/150‑1 (MQ=60)
                           TTGGCTGGCTTCCGGCGATCCTCATCAGTCACGGCTATAGCGAAGCACAGGCGGGTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAG                                                                                                                           <  SRR19973061.3759457/150‑1 (MQ=60)
                           TTGGCTGGCTTCCGGCGATCCTCATCAGTCACGGCTATAGCGAAGCACAGGCGGGTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAG                                                                                                                           <  SRR19973061.3759455/150‑1 (MQ=60)
                            TGGCTGGCTTCCGGCGATCCTCATCAGTCACGGCTATAGCGAAGCACAGGCGGGTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGC                                                                                                                          >  SRR19973061.1054015/1‑150 (MQ=60)
                               CTGGCTTCCGGCGATCCTCATCAGTCACGGCTATAGCGAAGCACAGGCGGGTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTT                                                                                                                       <  SRR19973061.640196/150‑1 (MQ=60)
                               CTGGCTTCCGGCGATCCTCATCAGTCACGGCTATAGCGAAGCACAGGCGGGTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTT                                                                                                                       <  SRR19973061.640376/150‑1 (MQ=60)
                                TGGCTTCCGGCGATCCTCATCAGTCACGGCTATAGCGAAGCACAGGCGGGTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTC                                                                                                                      <  SRR19973061.3174791/150‑1 (MQ=60)
                                    TTCCGGCGATCCTCATCAGTCACGGCTATAGCGAAGCACAGGCGGGTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTG                                                                                                                  >  SRR19973061.2663781/1‑150 (MQ=60)
                                    TTCCGGCGATCCTCATCAGTCACGGCTATAGCGAAGCACAGGCGGGTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTG                                                                                                                  >  SRR19973061.754410/1‑150 (MQ=60)
                                        GGCGATCCTCATCAGTCACGGCTATAGCGAAGCACAGGCGGGTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTT                                                                                                              <  SRR19973061.2555907/150‑1 (MQ=60)
                                                       TCACGGCTATAGCGAAGCACAGGCGGGTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGG                                                                                               >  SRR19973061.3748681/1‑150 (MQ=60)
                                                       TCACGGCTATAGCGAAGCACAGGCGGGTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGG                                                                                               <  SRR19973061.3216722/150‑1 (MQ=60)
                                                        CACGGCTATAGCGAAGCACAGGCGGGTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGC                                                                                              >  SRR19973061.3113925/1‑150 (MQ=60)
                                                         ACGGCTATAGCGAAGCACAGGCGGGTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCG                                                                                             <  SRR19973061.1971723/150‑1 (MQ=60)
                                                         ACGGCTATAGCGAAGCACAGGCGGGTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCG                                                                                             >  SRR19973061.1584810/1‑150 (MQ=60)
                                                         ACGGCTATAGCGAAGCACAGGCGGGTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCG                                                                                             >  SRR19973061.1582905/1‑150 (MQ=60)
                                                          CGGCTATAGCGAAGCACAGGCGGGTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGC                                                                                            >  SRR19973061.2496275/1‑150 (MQ=60)
                                                              TATAGCGAAGCACAGGCGGGTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTT                                                                                        <  SRR19973061.2538764/150‑1 (MQ=60)
                                                               ATAGCGAAGCACAGGCGGGTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTG                                                                                       >  SRR19973061.982898/1‑150 (MQ=60)
                                                               ATAGCGAAGCACAGGCGGGTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTG                                                                                       >  SRR19973061.1867686/1‑150 (MQ=60)
                                                                          CAGGCGGGTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTT                                                                            >  SRR19973061.2383448/1‑150 (MQ=60)
                                                                           AGGCGGGTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTA                                                                           >  SRR19973061.3548001/1‑150 (MQ=60)
                                                                            GGCGGGTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTAT                                                                          >  SRR19973061.2178774/1‑150 (MQ=60)
                                                                            GGCGGGGTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTAT                                                                          <  SRR19973061.3330768/150‑1 (MQ=60)
                                                                                GGTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCA                                                                      <  SRR19973061.1786022/150‑1 (MQ=60)
                                                                                GGTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCA                                                                      <  SRR19973061.1787765/150‑1 (MQ=60)
                                                                                 GTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAG                                                                     <  SRR19973061.3244959/150‑1 (MQ=60)
                                                                                  TTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGC                                                                    <  SRR19973061.3434474/150‑1 (MQ=60)
                                                                                     ACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCGGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCA                                                                 >  SRR19973061.1348378/1‑150 (MQ=60)
                                                                                             GTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGagatcggaagagcacacgtctgaactccagtcacgatcgatgatc                                                         >  SRR19973061.716578/1‑105 (MQ=60)
                                                                                               TTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACC                                                       >  SRR19973061.214669/1‑150 (MQ=60)
                                                                                                TGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCT                                                      <  SRR19973061.767871/150‑1 (MQ=60)
                                                                                                TGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCT                                                      <  SRR19973061.768094/150‑1 (MQ=60)
                                                                                                TGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCT                                                      <  SRR19973061.768120/150‑1 (MQ=60)
                                                                                                    GCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTGGAC                                                  >  SRR19973061.1901028/1‑150 (MQ=60)
                                                                                                    GCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTGGAC                                                  >  SRR19973061.3340716/1‑150 (MQ=60)
                                                                                                          AGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTGGACTCTGCT                                            <  SRR19973061.1438042/150‑1 (MQ=60)
                                                                                                             CACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTGGACTCTGCTTTT                                         <  SRR19973061.75839/150‑1 (MQ=60)
                                                                                                             CACAGCAGCACCCGGTTTGCTGATCCCACTTTTCGTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGGTGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTGGACTCTGCTTTT                                         <  SRR19973061.3431683/150‑1 (MQ=60)
                                                                                                               CAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTGGACTCTGCTTTTCG                                       >  SRR19973061.2732898/1‑150 (MQ=60)
                                                                                                                  CAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTGGACTCTGCTTTTCGGTT                                    <  SRR19973061.1333552/150‑1 (MQ=60)
                                                                                                                    GCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTGGACTCTGCTTTTCGGTTTT                                  <  SRR19973061.2897891/150‑1 (MQ=60)
                                                                                                                       CCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTGGACTCTGCTTTTCGGTTTTGGT                               >  SRR19973061.857412/1‑150 (MQ=60)
                                                                                                                       CCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTGGACTCTGCTTTTCGGTTTTGGT                               >  SRR19973061.2603751/1‑150 (MQ=60)
                                                                                                                         CGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTGGACTCTGCTTTTCGGTTTTGGTTC                             <  SRR19973061.3168383/150‑1 (MQ=60)
                                                                                                                         CGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTGGACTCTGCTTTTCGGTTTTGGTTC                             <  SRR19973061.2794601/150‑1 (MQ=60)
                                                                                                                         CGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTGGACTCTGCTTTTCGGTTTTGGTTC                             >  SRR19973061.3025791/1‑150 (MQ=60)
                                                                                                                               GCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTGGACTCTGCTTTTCGGTTTTGGTTCCGGCGC                       >  SRR19973061.3252533/1‑150 (MQ=60)
                                                                                                                                CTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTGGACTCTGCTTTTCGGTTTTGGTTCCGGCGCA                      >  SRR19973061.3067535/1‑150 (MQ=60)
                                                                                                                                  GATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTGGACTCTGCTTTTCGGTTTTGGTTCCGGCGCAAC                    <  SRR19973061.926603/150‑1 (MQ=60)
                                                                                                                                  GATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTGGACTCTGCTTTTCGGTTTTGGTTCCGGCGCAAC                    >  SRR19973061.1428196/1‑150 (MQ=60)
                                                                                                                                  GATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTGGACTCTGCTTTTCGGTTTTGGTTCCGGCGCAAC                    <  SRR19973061.2502336/150‑1 (MQ=60)
                                                                                                                                   ATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCGGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTGGACTCTGCTTTTCGGTTTTGGTTCCGGCGCAACA                   >  SRR19973061.3271544/1‑150 (MQ=60)
                                                                                                                                    TCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTGGACTCTGCTTTTCGGTTTTGGTTCCGGCGCAACAA                  >  SRR19973061.1969587/1‑150 (MQ=60)
                                                                                                                                     CCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATGGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCTCACGCGATCACCTGGACTCTGCTTTTCGGTTTTGGTTCCGGCGCAACAAT                 <  SRR19973061.2716010/150‑1 (MQ=60)
                                                                                                                                         CTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTGGACTCTGCTTTTCGGTTTTGGTTCCGGCGCAACAATGATA             >  SRR19973061.1740525/1‑150 (MQ=60)
                                                                                                                                         CTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTGGACTCTGCTTTTCGGTTTTGGTTCCGGCGCAACAATGATA             <  SRR19973061.706794/150‑1 (MQ=60)
                                                                                                                                         CTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGATGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTGGACTCTGCTTTTCGGTTTTGGTTCCGGCGCAACAATGATA             >  SRR19973061.1001612/1‑150 (MQ=60)
                                                                                                                                            TTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTGGACTCTGCTTTTCGGTTTTGGTTCCGGCGCAACAATGATACTG          <  SRR19973061.414989/150‑1 (MQ=60)
                                                                                                                                            TTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTGGACTCTGCTTTTCGGTTTTGGTTCCGGCGCAACAATGATACTG          <  SRR19973061.870241/150‑1 (MQ=60)
                                                                                                                                            GTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTGGACTCTGCTTTTCGGTTTTGGTTCCGGCGCAACAATGATACTG          <  SRR19973061.414729/150‑1 (MQ=60)
                                                                                                                                                 ACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTGGACTCTGCTTTTCGGTTTTGGTTCCGGCGCAACAATGATACTGGGGTT     <  SRR19973061.2284062/150‑1 (MQ=60)
                                                                                                                                                 ACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTGGACTCTGCTTTTCGGTTTTGGTTCCGGCGCAACAATGATACTGGGGTT     <  SRR19973061.2284093/150‑1 (MQ=60)
                                                                                                                                                  CATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTGGACTCTGCTTTTCGGTTTTGGTTCCGGCGCAACAATGATACTGGGGTTG    <  SRR19973061.1661507/150‑1 (MQ=60)
                                                                                                                                                  CATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTGGACTCTGCTTTTCGGTTTTGGTTCCGGCGCAACAATGATACTGGGGTTG    >  SRR19973061.1308059/1‑150 (MQ=60)
                                                                                                                                                    TAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTGGACTCTGCTTTTCGGTTTTGGTTCCGGCGCAACAATGATACTGGGGTTGAC  <  SRR19973061.2769858/150‑1 (MQ=60)
                                                                                                                                                    TAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTGGACTCTGCTTTTCGGTTTTGGTTCCGGCGCAACAATGATACTGGGGTTGAC  <  SRR19973061.2769900/150‑1 (MQ=60)
                                                                                                                                                    TAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTGGACTCTGCTTTTCGGTTTTGGTTCCGGCGCAACAATGATACTGGGGTTGAC  <  SRR19973061.2905724/150‑1 (MQ=60)
                                                                                                                                                     |                                                                                                                                                    
TCAACTCACTGGTCTATTACGTGATTATTGGCTGGCTTCCGGCGATCCTCATCAGTCACGGCTATAGCGAAGCACAGGCGGGTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATCATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTGGACTCTGCTTTTCGGTTTTGGTTCCGGCGCAACAATGATACTGGGGTTGAC  >  CP009273/1870594‑1870891

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑