Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F534 I1 R1 1235 230.2 7611948 95.1% 7238962 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009273 2,043,643 T→A L1742Q (CTG→CAG)  yeeJ → putative adhesin

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,043,6430TA99.5% 669.5 / NA 204L1742Q (CTG→CAG) yeeJputative adhesin
Reads supporting (aligned to +/- strand):  ref base T (0/1);  new base A (94/109);  total (94/110)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 207 total aligned reads displayed.

CGGCAACCCGGTAGAAGGCATAAAAGTTAATTTCCGCGGAACCTCCGTCACGCTAAGCAGCACCAGCGTTGAAACGGATGATCGGGGTTTCGCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCTGGCAGATAAACCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTGCAGATGTTAATTCTGCGACGATTACCAGTCTGGAGATACCGGAAGGTCAGGTAATGGTCGCACAAGACGTAGCAGTTAAAGCTCACGTTAACGACCAGT  >  CP009273/2043497‑2043789
                                                                                                                                                  |                                                                                                                                                  
    aaCCCGGTAGAAGGCATAAAAGTTAATTTCCGCGGAACCTCCGTCACGCTAAGCAGCACCAGCGTTGAAACGGATGATCGGGGTTTCGCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCAGGCAGAt                                                                                                                                             <  1:2938827/150‑1 (MQ=255)
    aaCCCGGTAGAAGGCATAAAAGTTAATTTCCGCGGAACCTCCGTCACGCTAAGCAGCACCAGCGTTGAAACGGATGATCGGGGTTTCGCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCAGGCAGAt                                                                                                                                             >  1:2058684/1‑150 (MQ=255)
       ccGGTAGAAGGCATAAAAGTTAATTTCCGCGGAACCTCCGTCACGCTAAGCAGCACCAGCGTTGAAACGGATGATCGGGGTTTCGCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCAGGCAGATaaa                                                                                                                                          <  1:3275766/150‑1 (MQ=255)
        cGGTAGAAGGCATAAAAGTTAATTTCCGCGGAACCTCCGTCACGCTAAGCAGCACCAGCGTTGAAACGGATGATCGGGGTTTCGCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCAGGCAGATAAAc                                                                                                                                         >  1:2079892/1‑150 (MQ=255)
         ggTAGAAGGCATAAAAGTTAATTTCCGCGGAACCTCCGTCACGCTAAGCAGCACCAGCGTTGAAACGGATGATCGGGGTTTCGCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCAGGCAGATAAAcc                                                                                                                                        <  1:562554/150‑1 (MQ=255)
           tAGAAGGCATAAAAGTTAATTTCCGCGGAACCTCCGTCACGCTAAGCAGCACCAGCGTTGAAACGGATGATCGGGGTTTCGCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCAGGCAGATAAACCTa                                                                                                                                      <  2:2691906/150‑1 (MQ=255)
           tAGAAGGCATAAAAGTTAATTTCCGCGGAACCTCCGTCACGCTAAGCAGCACCAGCGTTGAAACGGATGATCGGGGTTTCGCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCAGGCAGATAAACCTa                                                                                                                                      <  2:3644018/150‑1 (MQ=255)
             gAAGGCATAAAAGTTAATTTCCGCGGAACCTCCGTCACGCTAAGCAGCACCAGCGTTGAAACGGATGATCGGGGTTTCGCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCAGGCAGATAAACCTACt                                                                                                                                    <  1:2386662/150‑1 (MQ=255)
             gAAGGCATAAAAGTTAATTTCCGCGGAACCTCCGTCACGCTAAGCAGCACCAGCGTTGAAACGGATGATCGGGGTTTCGCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCAGGCAGATAAACCTACt                                                                                                                                    >  1:909241/1‑150 (MQ=255)
              aaGGCATAAAAGTTAATTTCCGCGGAACCTCCGTCACGCTAAGCAGCACCAGCGTTGAAACGGATGATCGGGGTTTCGCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCAGGCAGATAAACCTACTg                                                                                                                                   <  2:1002267/150‑1 (MQ=255)
               aGGCATAAAAGTTAATTTCCGCGGAACCTCCGTCACGCTAAGCAGCACCAGCGTTGAAACGGATGATCGGGGTTTCGCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCAGGCAGATAAACCTACTGa                                                                                                                                  >  1:3473858/1‑150 (MQ=255)
               aGGCATAAAAGTTAATTTCCGCGGAACCTCCGTCACGCTAAGCAGCACCAGCGTTGAAACGGATGATCGGGGTTTCGCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCAGGCAGATAAACCTACTGa                                                                                                                                  >  2:1720656/1‑150 (MQ=255)
               aGGCATAAAAGTTAATTTCCGCGGAACCTCCGTCACGCTAAGCAGCACCAGCGTTGAAACGGATGATCGGGGTTTCGCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCAGGCAGATAAACCTACTGa                                                                                                                                  >  1:1574690/1‑150 (MQ=255)
                ggCATAAAAGTTAATTTCCGCGGAACCTCCGTCACGCTAAGCAGCACCAGCGTTGAAACGGATGATCGGGGTTTCGCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCAGGCAGATAAACCTACTGaa                                                                                                                                 <  2:2992687/150‑1 (MQ=255)
                 gCATAAAAGTTAATTTCCGCGGAACCTCCGTCACGCTAAGCAGCACCAGCGTTGAAACGGATGATCGGGGTTTCGCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCAGGCAGATAAACCTACTGAAg                                                                                                                                <  1:1981370/150‑1 (MQ=255)
                 gCATAAAAGTTAATTTCCGCGGAACCTCCGTCACGCTAAGCAGCACCAGCGTTGAAACGGATGATCGGGGTTTCGCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCAGGCAGATAAACCTACTGAAg                                                                                                                                <  2:33215/150‑1 (MQ=255)
                   aTAAAAGTTAATTTCCGCGGAACCTCCGTCACGCTAAGCAGCACCAGCGTTGAAACGGATGATCGGGGTTTCGCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCAGGCAGATAAACCTACTGAAGTc                                                                                                                              >  2:2531906/1‑150 (MQ=255)
                    tAAAAGTTAATGTCCGCGGAACCTCCGTCACGCTAAGCAGCACCAGCGTTGAAACGGATGATCGGGGTTTCGCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCAGGCAGATAAACCTACTGAAGTCa                                                                                                                             <  2:2441147/150‑1 (MQ=255)
                      aaaGTTAATTTCCGCGGAACCTCCGTCACGCTAAGCAGCACCAGCGTTGAAACGGATGATCGGGGTTTCGCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCAGGCAGATAAACCTACTGAAGTCAtc                                                                                                                           >  1:3754718/1‑150 (MQ=255)
                       aaGTTAATTTCCGCGGAACCTCCGTCACGCTAAGCAGCACCAGCGTTGAAACGGATGATCGGGGTTTCGCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCAGGCAGATAAACCTACTGAAGTCAtct                                                                                                                          <  1:989452/150‑1 (MQ=255)
                        aGTTAATTTCCGCGGAACCTCCGTCACGCTAAGCAGCACCAGCGTTGAAACGGATGATCGGGGTTTCGCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCAGGCAGATAAACCTACTGAAGTCAtctc                                                                                                                         <  2:2511840/150‑1 (MQ=255)
                        aGTTAATTTCCGCGGAACCTCCGTCACGCTAAGCAGCACCAGCGTTGAAACGGATGATCGGGGTTTCGCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCAGGCAGATAAACCTACTGAAGTCAtctc                                                                                                                         >  2:2408201/1‑150 (MQ=255)
                         gTTAATTTCCGCGGAACCTCCGTCACGCTAAGCAGCACCAGCGTTGAAACGGATGATCGGGGTTTCGCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCAGGCAGATAAACCTACTGAAGTCATCTcg                                                                                                                        >  1:3442608/1‑150 (MQ=255)
                            aaTTTCCGCGGAACCTCCGTCACGCTAAGCAGCACCAGCGTTGAAACGGATGATCGGGGTTTCGCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCAGGCAGATAAACCTACTGAAGTCATCTCGCGa                                                                                                                     <  1:1702004/150‑1 (MQ=255)
                            aaTTTCCGCGGAACCTCCGTCACGCTAAGCAGCACCAGCGTTGAAACGGATGATCGGGGTTTCGCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCAGGCAGATAAACCTACTGAAGTCATCTCGCGa                                                                                                                     >  1:517394/1‑150 (MQ=255)
                            aaTTTCCGCGGAACCTCCGTCACGCTAAGCAGCACCAGCGTTGAAACGGATGATCGGGGTTTCGCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCAGGCAGATAAACCTACTGAAGTCATCTCGCGa                                                                                                                     >  2:2736569/1‑150 (MQ=255)
                                  cgcgGAACCTCCGTCACGCTAAGCAGCACCAGCGTTGAAACGGATGATCGGGGTTTCGCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCAGGCAGATAAACCTACTGAAGTCATCTCGCGATTACTg                                                                                                               >  2:2081966/1‑150 (MQ=255)
                                    cgGAACCTCCGTCACGCTAAGCAGCACCAGCGTTGAAACGGATGATCGGGGTTTCGCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCAGGCAGATAAACCTACTGAAGTCATCTCGCGATTACTGaa                                                                                                             >  2:2777545/1‑150 (MQ=255)
                                    cgGAACCTCCGTCACGCTAAGCAGCACCAGCGTTGAAACGGATGATCGGGGTTTCGCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCAGGCAGATAAACCTACTGAAGTCATCTCGCGATTACTGaa                                                                                                             >  2:392373/1‑150 (MQ=255)
                                    cgGAACCTCCGTCACGCTAAGCAGCACCAGCGTTGAAACGGATGATCGGGGTTTCGCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCAGGCAGATAAACCTACTGAAGTCATCTCGCGATTACTGaa                                                                                                             >  2:1367791/1‑150 (MQ=255)
                                       aaCCTCCGTCACGCTAAGCAGCACCAGCGTTGAAACGGATGATCGGGGTTTCGCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCAGGCAGATAAACCTACTGAAGTCATCTCGCGATTACTGAATGc                                                                                                          >  2:2184872/1‑150 (MQ=255)
                                       aaCCTCCGTCACGCTAAGCAGCACCAGCGTTGAAACGGATGATCGGGGTTTCGCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCAGGCAGATAAACCTACTGAAGTCATCTCGCGATTACTGAATGc                                                                                                          >  2:830844/1‑150 (MQ=255)
                                           tCCGTCACGCTAAGCAGCACCAGCGTTGAAACGGATGATCGGGGTTTCGCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCAGGCAGATAAACCTACTGAAGTCATCTCGCGATTACTGAATGCCAGt                                                                                                      <  1:229972/150‑1 (MQ=255)
                                            ccGTCACGCTAAGCAGCACCAGCGTTGAAACGGATGATCGGGGTTTCGCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCAGGCAGATAAACCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTg                                                                                                     <  1:1697840/150‑1 (MQ=255)
                                            ccGTCACGCTAAGCAGCACCAGCGTTGAAACGGATGATCGGGGTTTCGCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCAGGCAGATAAACCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTg                                                                                                     <  1:3752700/150‑1 (MQ=255)
                                              gTCACGCTAAGCAGCACCAGCGTTGAAACGGATGATCGGGGTTTCGCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCAGGCAGATAAACCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTGCa                                                                                                   >  2:33958/1‑150 (MQ=255)
                                              gTCACGCTAAGCAGCACCAGCGTTGAAACGGATGATCGGGGTTTCGCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCAGGCAGATAAACCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTGCa                                                                                                   >  1:1755487/1‑150 (MQ=255)
                                                 aCGCTAAGCAGCACCAGCGTTGAAACGGATGATCGGGGTTTCGCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCAGGCAGATAAACCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTGCAGAt                                                                                                <  2:3730120/150‑1 (MQ=255)
                                                 aCGCTAAGCAGCACCAGCGTTGAAACGGATGATCGGGGTTTCGCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCAGGCAGATAAACCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTGCAGAt                                                                                                >  1:1900523/1‑150 (MQ=255)
                                                    cTAAGCAGCACCAGCGTTGAAACGGATGATCGGGGTTTCGCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCAGGCAGATAAACCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTGCAGATGtt                                                                                             <  1:3444673/150‑1 (MQ=255)
                                                        gcagcaCCAGCGTTGAAACGGATGATCGGGGTTTCGCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCAGGCAGATAAACCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTGCAGATGTTAAtt                                                                                         <  1:44116/150‑1 (MQ=255)
                                                          agcaCCAGCGTTGAAACGGATGATCGGGGTTTCGCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCAGGCAGATAAACCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTGCAGATGTTAATTCt                                                                                       <  2:770287/150‑1 (MQ=255)
                                                           gcaCCAGCGTTGAAACGGATGATCGGGGTTTCGCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCAGGCAGATAAACCTACTGAAGTCATCTCGCGATTACTGTATGCCAGTGCAGATGTTAATTCTg                                                                                      <  2:543755/150‑1 (MQ=255)
                                                           gcaCCAGCGTTGAAACGGATGATCGGGGTTTCGCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCAGGCAGATAAACCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTGCAGATGTTAATTCTg                                                                                      <  2:1753433/150‑1 (MQ=255)
                                                            caCCAGCGTTGAAACGGATGATCGGGGTTTCGCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCAGGCAGATAAACCTACTGAAGTCATCTCGCGATTACTGAATACCAGTGCAGATGTTAATTCTGc                                                                                     >  1:3662588/1‑150 (MQ=255)
                                                             aCCAGCGTTGAAACGGATGATCGGGGTTTCGCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCAGGCAGATAAACCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTGCAGATGTTAATTCTGcg                                                                                    >  1:3726336/1‑150 (MQ=255)
                                                              ccAGCGTTGAAACGGATGATCGGGGTTTCGCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCAGGCAGATAAACCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTGCAGATGTTAATTCTGcga                                                                                   <  1:968350/150‑1 (MQ=255)
                                                               cAGCGTTGAAACGGATGATCGGGGTTTCGCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCAGGCAGATAAACCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTGCAGATGTTAATTCTGcgac                                                                                  <  1:696446/150‑1 (MQ=255)
                                                               cAGCGTTGAAACGGATGATCGGGGTTTCGCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCAGGCAGATAAACCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTGCAGATGTTAATTCTGcgac                                                                                  <  1:2306110/150‑1 (MQ=255)
                                                                aGCGTTGAAACGGATGATCGGGGTTTCGCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCAGGCAGATAAACCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTGCAGATGTTAATTCTGcgacg                                                                                 >  1:1005844/1‑150 (MQ=255)
                                                                aGCGTTGAAACGGATGATCGGGGTTTCGCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCAGGCAGATAAACCTACTGAAGTCATCTCGCGATTACTGAATGCCAGT‑CAGATGTTAATTCTGcgacga                                                                                >  2:3460109/1‑150 (MQ=255)
                                                                  cGTTGAAACGGATGATCGGGGTTTCGCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCAGGCAGATAAACCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTGCAGATGTTAATTCTGCGACGAt                                                                               <  1:2138533/150‑1 (MQ=255)
                                                                  cGTTGAAACGGATGATCGGGGTTTCGCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCAGGCAGATAAACCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTGCAGATGTTAATTCTGCGACGAt                                                                               >  2:1529732/1‑150 (MQ=255)
                                                                   gTTGAAACGGATGATCGGGGTTTCGCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCAGGCAGATAAACCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTGCAGATGTTAATTCTGCGACGAtt                                                                              >  2:3081087/1‑150 (MQ=255)
                                                                    ttGAAACGGATGATCGGGGTTTCGCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCAGGCAGATAAACCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTGCAGATGTTAATTCTGCGACGATTa                                                                             <  2:489180/150‑1 (MQ=255)
                                                                         aCGGATGATCGGGGTTTCGCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCAGGCAGATAAACCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTGCAGATGTTAATTCTGCGACGATTACCAGt                                                                        <  1:1607518/150‑1 (MQ=255)
                                                                          cGGATGATCGGGGTTTCGCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCAGGCAGATAAACCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTGCAGATGTTAATTCTGCGACGATTACCAGTc                                                                       >  1:952470/1‑150 (MQ=255)
                                                                                atCGGGGTTTCGCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCAGGCAGATAAACCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTGCAGATGTTAATTCTGCGACGATTACCAGTCTGgaga                                                                 <  2:2703615/150‑1 (MQ=255)
                                                                                  cGGGGTTTCGCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCAGGCAGATAAACCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTGCAGATGTTAATTCTGCGACGATTACCAGTCTGGAGATa                                                               <  2:1358440/150‑1 (MQ=255)
                                                                                  cGGGGTTTCGCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCAGGCAGATAAACCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTGCAGATGTTAATTCTGCGACGATTACCAGTCTGGAGATa                                                               >  2:2262713/1‑150 (MQ=255)
                                                                                  cGGGGTTTCGCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCAGGCAGATAAACCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTGCAGATGTTAATTCTGCGACGATTACCAGTCTGGAGATa                                                               <  1:3555761/150‑1 (MQ=255)
                                                                                   ggggTTTCGCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCAGGCAGATAAACCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTGCAGATGTTAATTCTGCGACGATTACCAGTCTGGAGATAc                                                              <  2:623363/150‑1 (MQ=255)
                                                                                    gggTTTCGCTGAAATTCTTGTGACAAGCACAGAGGTCGGACTGAAAACAGTTTCAGCCTCTCAGGCAGATAAACCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTGCAGATGTTAATTCTGCGACGATTACCAGTCTGGAGATAcc                                                             <  1:2875741/150‑1 (MQ=255)
                                                                                            cTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCAGGCAGATAAACCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTGCAGATGTTAATTCTGCGACGATTACCAGTCTGGAGATACCGGAAGGTc                                                     >  1:1066833/1‑150 (MQ=255)
                                                                                             tGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCAGGCAGATAAACCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTGCAGATGTTAATTCTGCGACGATTACCAGTCTGGAGATACCGGAAGGTCa                                                    <  2:1255775/150‑1 (MQ=255)
                                                                                             tGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCAGGCAGATAAACCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTGCAGATGTTAATTCTGCGACGATTACCAGTCTGGAGATACCGGAAGGTCa                                                    >  1:3088807/1‑150 (MQ=255)
                                                                                             tGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCAGGCAGATAAACCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTGCAGATGTTAATTCTGCGACGATTACCAGTCTGGAGATACCGGAAGGTCa                                                    >  1:619503/1‑150 (MQ=255)
                                                                                               aaaTTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCAGGCAGATAAACCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTGCAGATGTTAATTCTGCGACGATTACCAGTCTGGAGATACCGGAAGGTCAgg                                                  >  1:2320365/1‑150 (MQ=255)
                                                                                                 aTTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCAGGCAGATAAACCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTGCAGATGTTAATTCTGCGACGATTACCAGTCTGGAGATACCGGAAGGTCAGGTa                                                >  2:1446782/1‑150 (MQ=255)
                                                                                                            aaGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCAGGCAGATAAACCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTGCAGATGTTAATTCTGCGACGATTACCAGTCTGGAGATACCGGAAGGTCAGGTAATGGTCGcaca                                     >  2:824691/1‑150 (MQ=255)
                                                                                                            aaGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCAGGCAGATAAACCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTGCAGATGTTAATTCTGCGACGATTACCAGTCTGGAGATACCGGAAGGTCAGGTAATGGTCGcaca                                     <  1:266875/150‑1 (MQ=255)
                                                                                                               cACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCAGGCAGATAAACCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTGCAGATGTTAATTCTGCGACGATTACCAGTCTGGAGATACCGGAAGGTCAGGTAATGGTCGCACAAGa                                  >  2:203500/1‑150 (MQ=255)
                                                                                                                aCCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCAGGCAGATAAACCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTGCAGATGTTAATTCTGCGACGATTACCAGTCTGGAGATACCGGAAGGTCAGGTAATGGTCGCACAAGAc                                 >  1:1467669/1‑150 (MQ=255)
                                                                                                                aCCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCAGGCAGATAAACCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTGCAGATGTTAATTCTGCGACGATTACCAGTCTGGAGATACCGGAAGGTCAGGTAATGGTCGCACAAGAc                                 <  1:3127637/150‑1 (MQ=255)
                                                                                                                aCCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCAGGCAGATAAACCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTGCAGATGTTAATTCTGCGACGATTACCAGTCTGGAGATACCGGAAGGTCAGGTAATGGTCGCACAAGAc                                 >  1:382218/1‑150 (MQ=255)
                                                                                                                 ccGAGGTCGGACTGAAAACAGTTTCAGCCTCTCAGGCAGATAAACCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTGCAGATGTTAATTCTGCGACGATTACCAGTCTGGAGATACCGGAAGGTCAGGTAATGGTCGCACAAGACg                                <  1:937990/150‑1 (MQ=255)
                                                                                                                   gAGGTCGGACTGAAAACAGTTTCAGCCTCTCAGGCAGATAAACCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTGCAGATGTTAATTCTGCGACGATTACCAGTCTGGAGATACCGGAAGGTCAGGTAATGGTCGCACAAGACGTa                              <  1:2736747/150‑1 (MQ=255)
                                                                                                                    aGGTCGGACTGAAAACAGTTTCAGCCTCTCAGGCAGATAAACCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTGCAGATGTTAATTCTGCGACGATTACCAGTCTGGAGATACCGGAAGGTCAGGTAATGGTCGCACAAGACGTNg                             <  1:392373/150‑1 (MQ=255)
                                                                                                                       tCGGACTGAAAACAGTTTCAGCCTCTCAGGCAGATAAACCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTGCAGATGTTAATTCTGCGACGATTACCAGTCTGGAGATACCGGAAGGTCAGGTAATGGTCGCACAAGACGTAGCAg                          >  1:2639999/1‑150 (MQ=255)
                                                                                                                         ggACTGAAAACAGTTTCAGCCTCTCAGGCAGATAAACCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTGCAGATGTTAATTCTGCGACGATTACCAGTCTGGAGATACCGGAAGGTCAGGTAATGGTCGCACAAGACGTAGCAGtt                        <  2:3659763/150‑1 (MQ=255)
                                                                                                                         ggACTGAAAACAGTTTCAGCCTCTCAGGCAGATAAACCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTGCAGATGTTAATTCTGCGACGATTACCAGTCTGGAGATACCGGAAGGTCAGGTAATGGTCGCACAAGACGTAGCAGtt                        >  1:2953480/1‑150 (MQ=255)
                                                                                                                         ggACTGAAAACAGTTTCAGCCTCTCAGGCAGATAAACCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTGCAGATGTTAATTCTGCGACGATTACCAGTCTGGAGATACCGGAAGGTCAGGTAATGGTCGCACAAGACGTAGCAGtt                        >  2:1665195/1‑150 (MQ=255)
                                                                                                                          gACTGAAAACAGTTTCAGCCTCTCAGGCAGATAAACCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTGCAGATGTTAATTCTGCGACGATTACCAGTCTGGAGATACCGGAAGGTCAGGTAATGGTCGCACAAGACGTAGCAGTTa                       <  1:76056/150‑1 (MQ=255)
                                                                                                                          gACTGAAAACAGTTTCAGCCTCTCAGGCAGATAAACCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTGCAGATGTTAATTCTGCGACGATTACCAGTCTGGAGATACCGGAAGGTCAGGTAATGGTCGCACAAGACGTAGCAGTTa                       <  2:680451/150‑1 (MQ=255)
                                                                                                                          gACTGAAAACAGTTTCAGCCTCTCAGGCAGATAAACCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTGCAGATGTTAATTCTGCGACGATTACCAGTCTGGAGATACCGGAAGGTCAGGTAATGGTCGCACAAGACGTAGCAGTTa                       <  2:3442608/150‑1 (MQ=255)
                                                                                                                           aCTGAAAACAGTTTCAGCCTCTCAGGCAGATAAACCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTGCAGATGTTAATTCTGCGACGATTACCAGTCTGGAGATACCGGAAGGTCAGGTAATGGTCGCACAAGACGTAGCAGTTaa                      <  2:3486832/150‑1 (MQ=255)
                                                                                                                             tGAAAACAGTTTCAGCCTCTCAGGCAGATAAACCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTGCAGATGTTAATTCTGCGACGATTACCAGTCTGGAGATACCGGAAGGTCAGGTAATGGTCGCACAAGACGTAGCAGTTAAAg                    >  2:2027887/1‑150 (MQ=255)
                                                                                                                             tGAAAACAGTTTCAGCCTCTCAGGCAGATAAACCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTGCAGATGTTAATTCTGCGACGATTACCAGTCTGGAGATACCGGAAGGTCAGGTAATGGTCGCACAAGACGTAGCAGTTAAAg                    <  2:3236518/150‑1 (MQ=255)
                                                                                                                                aaaCAGTTTCAGCCTCTCAGGCAGATAAACCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTGCAGATGTTAATTCTGCGACGATTACCAGTCTGGAGATACCGGAAGGTCAGGTAATGGTCGCACAAGACGTAGCAGTTAAAGCTc                 >  2:1761049/1‑150 (MQ=255)
                                                                                                                                aaaCAGTTTCAGCCTCTCAGGCAGATAAACCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTGCAGATGTTAATTCTGCGACGATTACCAGTCTGGAGATACCGGAAGGTCAGGTAATGGTCGCACAAGACGTAGCAGTTAAAGCTc                 >  2:918869/1‑150 (MQ=255)
                                                                                                                                  aCAGTTTCAGCCTCTCAGGCAGATAAACCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTGCAGATGTTAATTCTGCGACGATTACCAGTCTGGAGATACCGGAAGGTCAGGTAATGGTCGCACAAGACGTAGCAGTTAAAGCTCAc               <  1:2530054/150‑1 (MQ=255)
                                                                                                                                   cAGTTTCAGCCTCTCAGGCAGATAAACCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTGCAGATGTTAATTCTGCGACGATTACCAGTCTGGAGATACCGGAAGGTCAGGTAATGGTCGCACAAGACGTAGCAGTTAAAGCTCACg              <  1:3236942/150‑1 (MQ=255)
                                                                                                                                   cAGTTTCAGCCTCTCAGGCAGATAAACCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTGCAGATGTTAATTCTGCGACGATTACCAGTCTGGAGATACCGGAAGGTCAGGTAATGGTCGCACAAGACGTAGCAGTTAAAGCTCACg              <  1:1629162/150‑1 (MQ=255)
                                                                                                                                     gTTTCAGCCTCTCAGGCAGATAAACCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTGCAGATGTTAATTCTGCGACGATTACCAGTCTGGAGATACCGGAAGGTCAGGTAATGGTCGCACAAGACGTAGCAGTTAAAGCTCACGtt            <  1:2777545/150‑1 (MQ=255)
                                                                                                                                     gTTTCAGCCTCTCAGGCAGATAAACCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTGCAGATGTTAATTCTGCGACGATTACCAGTCTGGAGATACCGGAAGGTCAGGTAATGGTCGCACAAGACGTAGCAGTTAAAGCTCACGtt            <  2:3053810/150‑1 (MQ=255)
                                                                                                                                      tttCAGCCTCTCAGGCAGATAAACCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTGCAGATGTTAATTCTGCGACGATTACCAGTCTGGAGATACCGGAAGGTCAGGTAATGGTCGCACAAGACGTAGCAGTTAAAGCTCACGTTa           <  1:1139275/150‑1 (MQ=255)
                                                                                                                                        tCAGCCTCTCAGGCAGATAAACCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTGCAGATGTTAATTCTGCGACGATTACCAGTCTGGAGATACCGGAAGGTCAGGTAATGGTCGCACAAGACGTAGCAGTTAAAGCTCACGTTAAc         <  2:2657982/150‑1 (MQ=255)
                                                                                                                                           gCCTCTCAGGCAGATAAACCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTGCAGATGTTAATTCTGCGACGATTACCAGTCTGGAGATACCGGAAGGTCAGGTAATGGTCGCACAAGACGTAGCAGTTAAAGCTCACGTTAACGAc      >  2:2939378/1‑150 (MQ=255)
                                                                                                                                            ccTCTCAGGCAGATAAACCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTGCAGATGTTAATTCTGCGACGATTACCAGTCTGGAGATACCGGAAGGACAGGTAATGGTCGCACAAGACGTAGCAGTTAAAGCTCACGTTAACGAcc     >  2:2007024/1‑150 (MQ=255)
                                                                                                                                              tctcaGGCAGATAAACCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTGCAGATGTTAATTCTGCGACGATTACCAGTCTGGAGATACCGGAAGGTCAGGTAATGGTCGCACAAGACGTAGCAGTTAAAGCTCACGTTAACGACCAg   >  2:674107/1‑150 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
CGGCAACCCGGTAGAAGGCATAAAAGTTAATTTCCGCGGAACCTCCGTCACGCTAAGCAGCACCAGCGTTGAAACGGATGATCGGGGTTTCGCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCTGGCAGATAAACCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTGCAGATGTTAATTCTGCGACGATTACCAGTCTGGAGATACCGGAAGGTCAGGTAATGGTCGCACAAGACGTAGCAGTTAAAGCTCACGTTAACGACCAGT  >  CP009273/2043497‑2043789

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TAACGGCAACCCGGTAGAAGGCATAAAAGTTAATTTCCGCGGAACCTCCGTCACGCTAAGCAGCACCAGCGTTGAAACGGATGATCGGGGTTTCGCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCTGGCAGATAAACCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTGCAGATGTTAATTCTGCGACGATTACCAGTCTGGAGATACCGGAAGGTCAGGTAATGGTCGCACAAGACGTAGCAGTTAAAGCTCACGTTAACGACCAGTTTG  >  CP009273/2043494‑2043792
                                                                                                                                                     |                                                                                                                                                     
TAACGGCAACCCGGTAGAAGGCATAAAAGTTAATTTCCGCGGAACCTCCGTCACGCTAAGCAGCACCAGCGTTGAAACGGATGATCGGGGTTTCGCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCA                                                                                                                                                       >  SRR19973061.2004827/1‑150 (MQ=60)
 ANCGGCAACCCGGTAGAAGGCATAAAAGTTAATTTCCGCGGAACCTCCGTCACGCTAAGCAGCACCAGCGTTGAAACGGATGATCGGGGTTTCGCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCAG                                                                                                                                                      >  SRR19973061.1789598/1‑150 (MQ=60)
    GGCAACCCGGTAGAAGGCATAAAAGTTAATTTCCGCGGAACCTCCGTCACGCTAAGCAGCACCAGCGTTGAAACGGATGATCGGGGTTTCGCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCAGGCA                                                                                                                                                   <  SRR19973061.2167271/150‑1 (MQ=60)
      CAACCCGGTAGAAGGCATAAAAGTTAATTTCCGCGGAACCTCCGTCACGCTAAGCAGCACCAGCGTTGAAACGGATGATCGGGGTTTCGCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCAGGCAGA                                                                                                                                                 <  SRR19973061.1281901/150‑1 (MQ=60)
      CAACCCGGTAGAAGGCATAAAAGTTAATTTCCGCGGAACCTCCGTCACGCTAAGCAGCACCAGCGTTGAAACGGATGATCGGGGTTTCGCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCAGGCAGA                                                                                                                                                 >  SRR19973061.3669118/1‑150 (MQ=60)
       AACCCGGTAGAAGGCATAAAAGTTAATTTCCGCGGAACCTCCGTCACGCTAAGCAGCACCAGCGTTGAAACGGATGATCGGGGTTTCGCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCAGGCAGAT                                                                                                                                                <  SRR19973061.2946389/150‑1 (MQ=60)
       AACCCGGTAGAAGGCATAAAAGTTAATTTCCGCGGAACCTCCGTCACGCTAAGCAGCACCAGCGTTGAAACGGATGATCGGGGTTTCGCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCAGGCAGAT                                                                                                                                                >  SRR19973061.2063865/1‑150 (MQ=60)
        ACCCGGTAGAAGGCATAAAAGTTAATTTCCGCGGAACCTCCGTCACGCTAAGCAGCACCAGCGTTGAAACGGATGATCGGGGTTTCGCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCAGGCAGATA                                                                                                                                               <  SRR19973061.3757720/150‑1 (MQ=60)
          CCGGTAGAAGGCATAAAAGTTAATTTCCGCGGAACCTCCGTCACGCTAAGCAGCACCAGCGTTGAAACGGATGATCGGGGTTTCGCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCAGGCAGATAAA                                                                                                                                             <  SRR19973061.3284202/150‑1 (MQ=60)
           CGGTAGAAGGCATAAAAGTTAATTTCCGCGGAACCTCCGTCACGCTAAGCAGCACCAGCGTTGAAACGGATGATCGGGGTTTCGCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCAGGCAGATAAAC                                                                                                                                            >  SRR19973061.2085110/1‑150 (MQ=60)
            GGTAGAAGGCATAAAAGTTAATTTCCGCGGAACCTCCGTCACGCTAAGCAGCACCAGCGTTGAAACGGATGATCGGGGTTTCGCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCAGGCAGATAAACC                                                                                                                                           <  SRR19973061.564028/150‑1 (MQ=60)
            GGTAGAAGGCATAAAAGTTAATTTCCGCGGAACCTCCGTCACGCTAAGCAGCACCAGCGTTGAAACGGATGATCGGGGTTTCGCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCAGGCAGATAAACC                                                                                                                                           <  SRR19973061.1978223/150‑1 (MQ=60)
                GAAGGCATAAAAGTTAATTTCCGCGGAACCTCCGTCACGCTAAGCAGCACCAGCGTTGAAACGGATGATCGGGGTTTCGCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCAGGCAGATAAACCTACT                                                                                                                                       >  SRR19973061.911575/1‑150 (MQ=60)
                GAAGGCATAAAAGTTAATTTCCGCGGAACCTCCGTCACGCTAAGCAGCACCAGCGTTGAAACGGATGATCGGGGTTTCGCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCAGGCAGATAAACCTACT                                                                                                                                       <  SRR19973061.2392747/150‑1 (MQ=60)
                  AGGCATAAAAGTTAATTTCCGCGGAACCTCCGTCACGCTAAGCAGCACCAGCGTTGAAACGGATGATCGGGGTTTCGCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCAGGCAGATAAACCTACTGA                                                                                                                                     >  SRR19973061.3482750/1‑150 (MQ=60)
                  AGGCATAAAAGTTAATTTCCGCGGAACCTCCGTCACGCTAAGCAGCACCAGCGTTGAAACGGATGATCGGGGTTTCGCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCAGGCAGATAAACCTACTGA                                                                                                                                     >  SRR19973061.1578603/1‑150 (MQ=60)
                  AGGCATAAAAGTTAATTTCCGCGGAACCTCCGTCACGCTAAGCAGCACCAGCGTTGAAACGGATGATCGGGGTCTCGCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCAGGCAGATAAACCTACTGA                                                                                                                                     <  SRR19973061.3021153/150‑1 (MQ=60)
                    GCATAAAAGTTAATTTCCGCGGAACCTCCGTCACGCTAAGCAGCACCAGCGTTGAAACGGATGATCGGGGTTTCGCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCAGGCAGATAAACCTACTGAAG                                                                                                                                   <  SRR19973061.1986327/150‑1 (MQ=60)
                     CATAAAAGTTAATTTCCGCGGAACCTCCGTCACGCTAAGCAGCACCAGCGTTGAAACGGATGATCGGGGTTTCGCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCAGGCAGATAAACCTACTGAAGT                                                                                                                                  >  SRR19973061.3483662/1‑150 (MQ=60)
                     CATAAAAGTTAATTTCCGCGGAACCTCCGTCACGCTAAGCAGCACCAGCGTTGAAACGGATGATCGGGGTTTCGCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCAGGCAGATAAACCTACTGAAGT                                                                                                                                  >  SRR19973061.1548117/1‑150 (MQ=60)
                         AAAGTTAATTTCCGCGGAACCTCCGTCACGCTAAGCAGCACCAGCGTTGAAACGGATGATCGGGGTTTCGCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCAGGCAGATAAACCTACTGAAGTCATC                                                                                                                              >  SRR19973061.3762895/1‑150 (MQ=60)
                         AAAGTTAATTTCCGCGGAACCTCCGTCACGCTAAGCAGCACCAGCGTTGAAACGGATGATCGGGGTTTCGCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCAGGCAGATAAACCTACTGAAGTCATC                                                                                                                              >  SRR19973061.3764326/1‑150 (MQ=60)
                          AAGTTAATTTCCGCGGAACCTCCGTCACGCTAAGCAGCACCAGCGTTGAAACGGATGATCGGGGTTTCGCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCAGGCAGATAAACCTACTGAAGTCATCT                                                                                                                             <  SRR19973061.991977/150‑1 (MQ=60)
                          AAGTTAATTTCCGCGGAACCTCCGTCACGCTAAGCAGCACCAGCGTTGAAACGGATGATCGGGGTTTCGCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCAGGCAGATAAACCTACTGAAGTCATCT                                                                                                                             <  SRR19973061.992077/150‑1 (MQ=60)
                            GTTAATTTCCGCGGAACCTCCGTCACGCTAAGCAGCACCAGCGTTGAAACGGATGATCGGGGTTTCGCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCAGGCAGATAAACCTACTGAAGTCATCTCG                                                                                                                           >  SRR19973061.3452230/1‑150 (MQ=60)
                            GTTAATTTCCGCGGAACCTCCGTCACGCTAAGCAGCACCAGCGTTGAAACGGATGATCGGGGTTTCGCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCAGGCAGATAAACCTACTGAAGTCATCTCG                                                                                                                           >  SRR19973061.3451443/1‑150 (MQ=60)
                               AATTTCCGCGGAACCTCCGTCACGCTAAGCAGCACCAGCGTTGAAACGGATGATCGGGGTTTCGCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCAGGCAGATAAACCTACTGAAGTCATCTCGCGA                                                                                                                        <  SRR19973061.1706279/150‑1 (MQ=60)
                               AATTTCCGCGGAACCTCCGTCACGCTAAGCAGCACCAGCGTTGAAACGGATGATCGGGGTTTCGCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCAGGCAGATAAACCTACTGAAGTCATCTCGCGA                                                                                                                        >  SRR19973061.518753/1‑150 (MQ=60)
                                    CCGCGGAACCTCCGTCACGCTAAGCAGCACCAGCGTTGAAACGGATGATCGGGGTTTCGCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCAGGCAGATAAACCTACTGAAGTCATCTCGCGATTACT                                                                                                                   <  SRR19973061.461237/150‑1 (MQ=60)
                                          AACCTCCGTCACGCTAAGCAGCACCAGCGTTGAAACGGATGATCGGGGTTTCGCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCAGGCAGATAAACCTACTGAAGTCATCTCGCGATTACTGAATGC                                                                                                             >  SRR19973061.161581/1‑150 (MQ=60)
                                          AACCTCCGTCACGCTAAGCAGCACCAGCGTTGAAACGGATGATCGGGGTTTCGCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCAGGCAGATAAACCTACTGAAGTCATCTCGCGATTACTGAATGC                                                                                                             >  SRR19973061.161243/1‑150 (MQ=60)
                                             CTCCGTCACGCTAAGCAGCACCAGCGTTGAAACGGATGATCGGGGTTTCGCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCAGGCAGATAAACCTACTGAAGTCATCTCGCGATTACTGAATGCCAG                                                                                                          >  SRR19973061.2664810/1‑150 (MQ=60)
                                              TCCGTCACGCTAAGCAGCACCAGCGTTGAAACGGATGATCGGGGTTTCGCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCAGGCAGATAAACCTACTGAAGTCATCTCGCGATTACTGAATGCCAGT                                                                                                         <  SRR19973061.230644/150‑1 (MQ=60)
                                               CCGTCACGCTAAGCAGCACCAGCGTTGAAACGGATGATCGGGGTTTCGCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCAGGCAGATAAACCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTG                                                                                                        <  SRR19973061.1702101/150‑1 (MQ=60)
                                               CCGTCACGCTAAGCAGCACCAGCGTTGAAACGGATGATCGGGGTTTCGCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCAGGCAGATAAACCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTG                                                                                                        <  SRR19973061.3762305/150‑1 (MQ=60)
                                                 GTCACGCTAAGCAGCACCAGCGTTGAAACGGATGATCGGGGTTTCGCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCAGGCAGATAAACCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTGCA                                                                                                      >  SRR19973061.1759888/1‑150 (MQ=60)
                                                    ACGCTAAGCAGCACCAGCGTTGAAACGGATGATCGGGGTTTCGCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCAGGCAGATAAACCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTGCAGAT                                                                                                   <  SRR19973061.2263279/150‑1 (MQ=60)
                                                    ACGCTAAGCAGCACCAGCGTTGAAACGGATGATCGGGGTTTCGCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCAGGCAGATAAACCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTGCAGAT                                                                                                   >  SRR19973061.1905233/1‑150 (MQ=60)
                                                     CGCTAAGCAGCACCAGCGTTGAAACGGATGATCGGGGTTTCGCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCAGGCAGATAAACCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTGCAGATG                                                                                                  <  SRR19973061.1888121/150‑1 (MQ=60)
                                                       CTAAGCAGCACCAGCGTTGAAACGGATGATCGGGGTTTCGCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCAGGCAGATAAACCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTGCAGATGTT                                                                                                <  SRR19973061.3453513/150‑1 (MQ=60)
                                                        TAAGCAGCACCAGCGTTGAAACGGATGATCGGGGTTTCGCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCAGGCAGATAAACCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTGCAGATGTTA                                                                                               >  SRR19973061.983085/1‑150 (MQ=60)
                                                           GCAGCACCAGCGTTGAAACGGATGATCGGGGTTTCGCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCAGGCAGATAAACCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTGCAGATGTTAATT                                                                                            <  SRR19973061.44283/150‑1 (MQ=60)
                                                            CAGCACCAGCGTTGAAACGGATGATCGGGGTTTCGCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCAGGCAGATAAACCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTGCAGATGTTAATTC                                                                                           <  SRR19973061.542710/150‑1 (MQ=60)
                                                             AGCACCAGCGTTGAAACGGATGATCGGGGTTTCGCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCAGGCAGATAAACCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTGCAGATGTTAATTNT                                                                                          <  SRR19973061.1101487/150‑1 (MQ=60)
                                                             AGCACCAGCGTTGAAACGGATGATCGGGGTTTCGCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCAGGCAGATAAACCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTGCAGATGTTAATTCT                                                                                          <  SRR19973061.563816/150‑1 (MQ=60)
                                                             AGCACCAGCGTTGAAACGGATGATCGGGGTTTCGCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCAGGCAGATAAACCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTGCAGATGTTAATTCT                                                                                          <  SRR19973061.3101817/150‑1 (MQ=60)
                                                               CACCAGCGTTGAAACGGATGATCGGGGTTTCGCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCAGGCAGATAAACCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTGCAGATGTTAATTCTGC                                                                                        >  SRR19973061.3671998/1‑150 (MQ=60)
                                                               CACCAGCGTTGAAACGGATGATCGGGGTTTCGCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCAGGCAGATAAACCTACTGAAGTCATCTCGCGATTACTGAATACCAGTGCAGATGTTAATTCTGC                                                                                        >  SRR19973061.3671951/1‑150 (MQ=60)
                                                                ACCAGCGTTGAAACGGATGATCGGGGTTTCGCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCAGGCAGATAAACCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTGCAGATGTTAATTCTGCG                                                                                       >  SRR19973061.3735862/1‑150 (MQ=60)
                                                                ACCAGCGTTGAAACGGATGATCGGGGTTTCGCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCAGGCAGATAAACCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTGCAGATGTTAATTCTGCG                                                                                       <  SRR19973061.2735755/150‑1 (MQ=60)
                                                                 CCAGCGTTGAAACGGATGATCGGGGTTTCGCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCAGGCAGATAAACCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTGCAGATGTTAATTCTGCGA                                                                                      <  SRR19973061.970800/150‑1 (MQ=60)
                                                                  CAGCGTTGAAACGGATGATCGGGGTTTCGCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCAGGCAGATAAACCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTGCAGATGTTAATTCTGCGAC                                                                                     <  SRR19973061.698259/150‑1 (MQ=60)
                                                                  CAGCGTTGAAACGGATGATCGGGGTTTCGCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCAGGCAGATAAACCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTGCAGATGTTAATTCTGCGAC                                                                                     >  SRR19973061.1693408/1‑150 (MQ=60)
                                                                  CAGCGTTGAAACGGATGATCGGGGTTTCGCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCAGGCAGATAAACCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTGCAGATGTTAATTCTGCGAC                                                                                     <  SRR19973061.2311954/150‑1 (MQ=60)
                                                                  CAGCGTTGAAACGGATGATCGGGGTTTCGCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCAGGCAGATAAACCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTGCAGATGTTAATTCTGCGAC                                                                                     <  SRR19973061.2311957/150‑1 (MQ=60)
                                                                   AGCGTTGAAACGGATGATCGGGGTTTCGCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCAGGCAGATAAACCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTGCAGATGTTAATTCTGCGACG                                                                                    >  SRR19973061.1008407/1‑150 (MQ=60)
                                                                   AGCGTTGAAACGGATGATCGGGGTTTCGCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCAGGCAGATAAACCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTGCAGATGTTAATTCTGCGACG                                                                                    <  SRR19973061.1703047/150‑1 (MQ=60)
                                                                     CGTTGAAACGGATGATCGGGGTTTCGCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCAGGCAGATAAACCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTGCAGATGTTAATTCTGCGACGAT                                                                                  <  SRR19973061.2927770/150‑1 (MQ=60)
                                                                     CGTTGAAACGGATGATCGGGGTTTCGCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCAGGCAGATAAACCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTGCAGATGTTAATTCTGCGACGAT                                                                                  <  SRR19973061.2143867/150‑1 (MQ=60)
                                                                     CGTTGAAACGGATGATCGGGGTTTCGCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCAGGCAGATAAACCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTGCAGATGTTAATTCTGCGACGAT                                                                                  <  SRR19973061.2143904/150‑1 (MQ=60)
                                                                      GTTGAAACGGATGATCGGGGTTTCGCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCAGGCAGATAAACCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTGCAGATGTTAATTCTGCGACGATT                                                                                 >  SRR19973061.932430/1‑150 (MQ=60)
                                                                         GAAACGGATGATCGGGGTTTCGCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCAGGCAGATAAACCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTGCAGATGTTAATTCTGCGACGATTACC                                                                              >  SRR19973061.2816269/1‑150 (MQ=60)
                                                                            ACGGATGATCGGGGTTTCGCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCAGGCAGATAAACCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTGCAGATGTTAATTCTGCGACGATTACCAGT                                                                           <  SRR19973061.1611519/150‑1 (MQ=60)
                                                                             CGGATGATCGGGGTTTCGCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCAGGCAGATAAACCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTGCAGATGTTAATTCTGCGACGATTACCAGTC                                                                          >  SRR19973061.954874/1‑150 (MQ=60)
                                                                              GGATGATCGGGGTTTCGCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCAGGCAGATAAACCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTGCAGATGTTAATTCTGCGACGATTACCAGTCT                                                                         <  SRR19973061.1754489/150‑1 (MQ=60)
                                                                                     CGGGGTTTCGCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCAGGCAGATAAACCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTGCAGATGTTAATTCTGCGACGATTACCAGTCTGGAGATA                                                                  <  SRR19973061.3564862/150‑1 (MQ=60)
                                                                                     CGGGGTTTCGCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCAGGCAGATAAACCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTGCAGATGTTAATTCTGCGACGATTACCAGTCTGGAGATA                                                                  <  SRR19973061.238690/150‑1 (MQ=60)
                                                                                      GGGGTTTCGCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCAGGCAGATAAACCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTGCAGATGTTAATTCTGCGACGATTACCAGTCTGGAGATAC                                                                 >  SRR19973061.593771/1‑150 (MQ=60)
                                                                                       GGGTTTCGCTGAAATTCTTGTGACAAGCACAGAGGTCGGACTGAAAACAGTTTCAGCCTCTCAGGCAGATAAACCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTGCAGATGTTAATTCTGCGACGATTACCAGTCTGGAGATACC                                                                <  SRR19973061.2883116/150‑1 (MQ=60)
                                                                                               CTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCAGGCAGATAAACCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTGCAGATGTTAATTCTGCGACGATTACCAGTCTGGAGATACCGGAAGGTC                                                        >  SRR19973061.1069573/1‑150 (MQ=60)
                                                                                               CTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCAGGCAGATAAACCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTGCAGATGTTAATTCTGCGACGATTACCAGTCTGGAGATACCGGAAGGTC                                                        <  SRR19973061.3813643/150‑1 (MQ=60)
                                                                                                TGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCAGGCAGATAAACCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTGCAGATGTTAATTCTGCGACGATTACCAGTCTGGAGATACCGGAAGGTCA                                                       >  SRR19973061.621087/1‑150 (MQ=60)
                                                                                                TGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCAGGCAGATAAACCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTGCAGATGTTAATTCTGCGACGATTACCAGTCTGGAGATACCGGAAGGTCA                                                       >  SRR19973061.3096770/1‑150 (MQ=60)
                                                                                                TGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCAGGCAGATAAACCTACTGAAGTCAACTCGCGATTACTGAATGCCAGAGCAGATGTTAATTCTGCGACGATTACCAGTCTGGAGATACCGGAAGGTCA                                                       >  SRR19973061.621122/1‑150 (MQ=60)
                                                                                                  AAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCAGGCAGATAAACCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTGCAGATGTTAATTCTGCGACGATTACCAGTCTGGAGATACCGGAAGGTCAGG                                                     >  SRR19973061.2326253/1‑150 (MQ=60)
                                                                                                   AATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCAGGCAGATAAACCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTGCAGATGTTAATTCTGCGACGATTACCAGTCTGGAGATACCGGAAGGTCAGGT                                                    <  SRR19973061.1046973/150‑1 (MQ=60)
                                                                                                      TCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCAGGCAGATAAACCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTGCAGATGTTAATTCTGCGACGATTACCAGTCTGGAGATACCGGAAGGTCAGGTAAT                                                 <  SRR19973061.2467580/150‑1 (MQ=60)
                                                                                                             ACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCAGGCAGATAAACCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTGCAGATGTTAATTCTGCGACGATTACCAGTCTGGAGATACCGGAATGTCAGTTAATTGTCGCA                                          >  SRR19973061.2309087/1‑150 (MQ=60)
                                                                                                               AAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCAGGCAGATAAACCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTGCAGATGTTAATTCTGCGACGATTACCAGTCTGGAGATACCGGAAGGTCAGGTAATGGTCGCACA                                        <  SRR19973061.2020768/150‑1 (MQ=60)
                                                                                                               AAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCAGGCAGATAAACCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTGCAGATGTTAATTCTGCGACGATTACCAGTCTGGAGATACCGGAAGGTCAGGTAATGGTCGCACA                                        <  SRR19973061.267611/150‑1 (MQ=60)
                                                                                                                 GCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCAGGCAGATAAACCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTGCAGATGTTAATTCTGCGACGATTACCAGTCTGGAGATACCGGAAGGTCAGGTAATGGTCGCACAAG                                      <  SRR19973061.9675/150‑1 (MQ=60)
                                                                                                                   ACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCAGGCAGATAAACCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTGCAGATGTTAATTCTGCGACGATTACCAGTCTGGAGATACCGGAAGGTCAGGTAATGGTCGCACAAGAC                                    >  SRR19973061.1471361/1‑150 (MQ=60)
                                                                                                                   ACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCAGGCAGATAAACCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTGCAGATGTTAATTCTGCGACGATTACCAGTCTGGAGATACCGGAAGGTCAGGTAATGGTCGCACAAGAC                                    >  SRR19973061.383262/1‑150 (MQ=60)
                                                                                                                   ACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCAGGCAGATAAACCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTGCAGATGTTAATTCTGCGACGATTACCAGTCTGGAGATACCGGAAGGTCAGGTAATGGTCGCACAAGAC                                    <  SRR19973061.3135694/150‑1 (MQ=60)
                                                                                                                    CCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCAGGCAGATAAACCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTGCAGATGTTAATTCTGCGACGATTACCAGTCTGGAGATACCGGAAGGTCAGGTAATGGTCGCACAAGACG                                   <  SRR19973061.940374/150‑1 (MQ=60)
                                                                                                                     CGAGGTCGGACTGAAAACAGTTTCAGCCTCTCAGGCAGATAAACCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTGCAGATGTTAATTCTGCGACGATTACCAGTCTGGAGATACCGGAAGGTCAGGTAATGGTCGCACAAGACGT                                  >  SRR19973061.399155/1‑150 (MQ=60)
                                                                                                                      GAGGTCGGACTGAAAACAGTTTCAGCCTCTCAGGCAGATAAACCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTGCAGATGTTAATTCTGCGACGATTACCAGTCTGGAGATACCGGAAGGTCAGGTAATGGTCGCACAAGACGTA                                 <  SRR19973061.2764144/150‑1 (MQ=60)
                                                                                                                      GAGGTCGGACTGAAAACAGTTTCAGCCTCTCAGGCAGATAAACCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTGCAGATGTTAATTCTGCGACGATTACCAGTCTGGAGATACCGGAAGGTCAGGTAATGGTCGCACAAGACGTA                                 <  SRR19973061.2743781/150‑1 (MQ=60)
                                                                                                                       AGGTCGGACTGAAAACAGTTTCAGCCTCTCAGGCAGATAAACCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTGCAGATGTTAATTCTGCGACGATTACCAGTCTGGAGATACCGGAAGGTCAGGTAATGGTCGCACAAGACGTNG                                <  SRR19973061.393444/150‑1 (MQ=60)
                                                                                                                       AGGTCGGACTGAAAACAGTTTCAGCCTCTCAGGCAGATAAACCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTGCAGATGTTAATTCTGCGACGATTACCAGTCTGGAGATACCGGAAGGTCAGGTAATGGTCGCACAAGACGTAG                                <  SRR19973061.393115/150‑1 (MQ=60)
                                                                                                                       AGGTCGGACTGAAAACAGTTTCAGCCTCTCAGGCAGATAAACCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTGCAGATGTTAATTCTGCGACGATTACCAGTCTGGAGATACCGGAAGGTCAGGTAATGGTCGCACAAGACGTAG                                <  SRR19973061.2078180/150‑1 (MQ=60)
                                                                                                                          TCGGACTGAAAACAGTTTCAGCCTCTCAGGCAGATAAACCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTGCAGATGTTAATTCTGCGACGATTACCAGTCTGGAGATACCGGAAGGTCAGGTAATGGTCGCACAAGACGTAGCAG                             >  SRR19973061.2646778/1‑150 (MQ=60)
                                                                                                                            GGACTGAAAACAGTTTCAGCCTCTCAGGCAGATAAACCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTGCAGATGTTAATTCTGCGACGATTACCAGTCTGGAGATACCGGAAGGTCAGGTAATGGTCGCACAAGACGTAGCAGTT                           >  SRR19973061.2961097/1‑150 (MQ=60)
                                                                                                                            GGACTGAAAACAGTTTCAGCCTCTCAGGCAGATAAACCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTGCAGATGTTAATTCTGCGACGATTACCAGTCTGGAGATACCGGAAGGTCAGGTAATGGTCGCACAAGACGTAGCAGTT                           >  SRR19973061.2961070/1‑150 (MQ=60)
                                                                                                                             GACTGAAAACAGTTTCAGCCTCTCAGGCAGATAAACCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTGCAGATGTTAATTCTGCGACGATTACCAGTCTGGAGATACCGGAAGGTCAGGTAATGGTCGCACAAGACGTAGCAGTTA                          <  SRR19973061.76332/150‑1 (MQ=60)
                                                                                                                             GACTGAAAACAGTTTCAGCCTCTCAGGCAGATAAACCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTGCAGATGTTAATTCTGCGACGATTACCAGTCTGGAGATACCGGAAGGTCAGGTAATGGTCGCACAAGACGTAGCAGTTA                          <  SRR19973061.3408089/150‑1 (MQ=60)
                                                                                                                             GACTGAAAACAGTTTCAGCCTCTCAGGCAGATAAACCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTGCAGATGTTAATTCTGCGACGATTACCAGTCTGGAGATACCGGAAGGTCAGGTAATGGTCGCACAAGACGTAGCAGTTA                          <  SRR19973061.3240849/150‑1 (MQ=60)
                                                                                                                              ACTGAAAACAGTTTCAGCCTCTCAGGCAGATAAACCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTGCAGATGTTAATTCTGCGACGATTACCAGTCTGGAGATACCGGAAGGTCAGGTAATGGTCGCACAAGACGTAGCAGTTAA                         >  SRR19973061.3257067/1‑150 (MQ=60)
                                                                                                                                   AAACAGTTTCAGCCTCTCAGGCAGATAAACCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTGCAGATGTTAATTCTGCGACGATTACCAGTCTGGAGATACCGGAAGGTCAGGTAATGGTCGCACAAGACGTAGCAGTTAAAGCTC                    >  SRR19973061.3463861/1‑150 (MQ=60)
                                                                                                                                    AACAGTTTCAGACTCTCAGGCAGATAAACCTACTGAAGTCATCTCGTGTTTACTGAATGCCAGTGCAGATGTTAATTCTGAGACGATTACCAGTCTGGAGATACCGGAAGGTCAGGTAATGGTCGCACAAGACGTAGCAGTTAAAGCTCA                   <  SRR19973061.319970/150‑1 (MQ=60)
                                                                                                                                     ACAGTTTCAGCCTCTCAGGCAGATAAACCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTGCAGATGTTAATTCTGCGACGATTACCAGTCTGGAGATACCGGAAGGTCAGGTAATGGTCGCACAAGACGTAGCAGTTAAAGCTCAC                  <  SRR19973061.2536500/150‑1 (MQ=60)
                                                                                                                                     ACAGTTTCAGCCTCTCAGGCAGATAAACCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTGCAGATGTTAATTCTGCGACGATTACCAGTCTGGAGATACCGGAAGGTCAGGTAATGGTCGCACAAGACGTAGCAGTTAAAGCTCAC                  <  SRR19973061.2538356/150‑1 (MQ=60)
                                                                                                                                     ACAGTTTCAGCCTCTCAGGCAGATAAACCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTGCAGATGTTAATTCTGCGACGATTACCAGTCTGGAGATACCGGAAGGTCAGGTAATGGTCGCACAAGACGTAGCAGTTAAAGCTCAC                  <  SRR19973061.944873/150‑1 (MQ=60)
                                                                                                                                      CAGTTTCAGCCTCTCAGGCAGATAAACCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTGCAGATGTTAATTCTGCGACGATTACCAGTCTGGAGATACCGGAAGGTCAGGTAATGGTCGCACAAGACGTAGCAGTTAAAGCTCACG                 <  SRR19973061.3245264/150‑1 (MQ=60)
                                                                                                                                      CAGTTTCAGCCTCTCAGGCAGATAAACCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTGCAGATGTTAATTCTGCGACGATTACCAGTCTGGAGATACCGGAAGGTCAGGTAATGGTCGCACAAGACGTAGCAGTTAAAGCTCACG                 <  SRR19973061.1633233/150‑1 (MQ=60)
                                                                                                                                        GTTTCAGCCTCTCAGGCAGATAAACCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTGCAGATGTTAATTCTGCGACGATTACCAGTCTGGAGATACCGGAAGGTCAGGTAATGGTCGCACAAGACGTAGCAGTTAAAGCTCACGTT               <  SRR19973061.2784690/150‑1 (MQ=60)
                                                                                                                                         TTTCAGCCTCTCAGGCAGATAAACCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTGCAGATGTTAATTCTGCGACGATTACCAGTCTGGAGATACCGGAAGGTCAGGTAATGGTCGCACAAGACGTAGCAGTTAAAGCTCACGTTA              <  SRR19973061.1142174/150‑1 (MQ=60)
                                                                                                                                           TCAGCCTCTCAGGCAGATAAACCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTGCAGATGTTAATTCTGCGACGATTACCAGTCTGGAGATACCGGAAGGTCAGGTAATGGTCGCACAAGACGTAGCAGTTAAAGCTCACGTTAAC            <  SRR19973061.802643/150‑1 (MQ=60)
                                                                                                                                                  CTCAGGCAGATAAACCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTGCAGATGTTAATTCTGCGACGATTACCAGTCTGGAGATACCGGAAGGTCAGGTAATGGTCGCACAAGACGTAGCAGTTAAAGCTCACGTTAACGACCAGT     >  SRR19973061.710930/1‑150 (MQ=60)
                                                                                                                                                   TCAGGCAGATAAACCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTGCAGATGTTAATTCTGCGACGATTACCAGTCTGGAGATACCGGAAGGTCAGGTAATGGTCGCACAAGACGTAGCAGTTAAAGCTCACGTTAACGACCAGTT    >  SRR19973061.2291201/1‑150 (MQ=60)
                                                                                                                                                   TCAGGCAGATAAACCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTGCAGATGTTAATTCTGCGACGATTACCAGTCTGGAGATACCGGAAGGTCAGGTAATGGTCGCACAAGACGTAGCAGTTAAAGCTCACGTTAACGACCAGTT    >  SRR19973061.2291114/1‑150 (MQ=60)
                                                                                                                                                     AGGCAGATAAACCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTGCAGATGTTAATTCTGCGACGATTACCAGTCTGGAGATACCGGAAGGTCAGGTAATGGTCGCACAAGACGTAGCAGTTAAAGCTCACGTTAACGACCAGTTTG  >  SRR19973061.3118249/1‑150 (MQ=60)
                                                                                                                                                     |                                                                                                                                                     
TAACGGCAACCCGGTAGAAGGCATAAAAGTTAATTTCCGCGGAACCTCCGTCACGCTAAGCAGCACCAGCGTTGAAACGGATGATCGGGGTTTCGCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCTGGCAGATAAACCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTGCAGATGTTAATTCTGCGACGATTACCAGTCTGGAGATACCGGAAGGTCAGGTAATGGTCGCACAAGACGTAGCAGTTAAAGCTCACGTTAACGACCAGTTTG  >  CP009273/2043494‑2043792

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑