Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F534 I1 R1 1235 230.2 7611948 95.1% 7238962 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009273 2,183,642 C→A D250Y (GAT→TAT)  yehB ← putative outer membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,183,6420CA99.6% 877.5 / NA 263D250Y (GAT→TAT) yehBputative outer membrane protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base A (149/113);  minor base T (0/1);  total (149/114)
Fisher's exact test for biased strand distribution p-value = 4.33e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 263 total aligned reads displayed.

AATCTGTAATCGCGAACGGGCCAGGAGGAACCTCTTTCTGATAAACCACAAAACCATTCTGTTCAATAGTTACCAGCGCGTTACTCTGAGCAATCCCCTGCACCCGTGGCGTAAAATTTTGTTTCGAGTTAGGCAACATCTGCATATCACGAAACAACCGCACACCTCTGAAGCGAACAGAATCAAAAATATCGCTTGATGTGTACATATCACCCACGCGAAGCGTGCCGAGAAGTTGGGCAAATCCACGTTCCAGATACAGGGTATTGCTTTTCCACACCCCTGGATTGTTA  >  CP009273/2183495‑2183787
                                                                                                                                                   |                                                                                                                                                 
aaTCTGTAATCGCGAACGGGCCAGGAGGAACCTCTTTCTGATAAACCACAAAACCATTCTGTTCAATAGTTACCAGCGCGTTACTCTGAGCAATCCCCTGCACCCGTGGCGTAAAATTTTGTTTCGAGTTAGGCAACATCTGCATATAAc                                                                                                                                                 >  1:1155234/1‑150 (MQ=255)
aaTCTGTAATCGCGAACGGGCCAGGAGGAACCTCTTTCTGATAAACCACAAAACCATTCTGTTCAATAGTTACCAGCGCGTTACTCTGAGCAATCCCCTGCACCCGTGGCGTAAAATTTTGTTTCGAGTTAGGCAACATCTGCATATAAc                                                                                                                                                 <  1:1977742/150‑1 (MQ=255)
    tGTAATCGCGAACGGGCCAGGAGGAACCTCTTTCTGATAAACCACAAAACCATTCTGTTCAATAGTTACCAGCGCGTTACTCTGAGCAATCCCCTGCACCCGTGGCGTAAAATTTTGTTTCGAGTTAGGCAACATCTGCATATAACGaaa                                                                                                                                             >  1:2217740/1‑150 (MQ=255)
      tAATCGCGAACGGGCCAGGAGGAACCTCTTTCTGATAAACCACAAAACCATTCTGTTCAATAGTTACCAGCGCGTTACTCTGAGCAATCCCCTGCACCCGTGGCGTAAAATTTTGTTTCGAGTTAGGCAACATCTGCATATAACGAaaca                                                                                                                                           <  2:3470932/150‑1 (MQ=255)
       aaTCGCGAACGGGCCAGGAGGAACCTCTTTCTGATAAACCACAAAACCATTCTGTTCAATAGTTACCAGCGCGTTACTCTGAGCAATCCCCTGCACCCGTGGCGTAAAATTTTGTTTCGAGTTAGGCAACATCTGCATATAACGAaacaa                                                                                                                                          >  1:3338392/1‑150 (MQ=255)
         tCGCGAACGGGCCAGGAGGAACCTCTTTCTGATAAACCACAAAACCATTCTGTTCAATAGTTACCAGCGCGTTACTCTGAGCAATCCCCTGCACCCGTGGCGTAAAATTTTGTTTCGAGTTAGGCAACATCTGCATATAACGAAACAAcc                                                                                                                                        <  1:1550270/150‑1 (MQ=255)
            cgAACGGGCCAGGAGGAACCTCTTTCTGATAAACCACAAAACCATTCTGTTCAATAGTTACCAGCGCGTTACTCTGAGCAATCCCCTGCACCCGTGGCGTAAAATTTTGTTTCGAGTTAGGCAACATCTGCATATAACGAAACAACCGca                                                                                                                                     >  1:1795877/1‑150 (MQ=255)
              aaCGGGCCAGGAGGAACCTCTTTCTGATAAACCACAAAACCATTCTGTTCAATAGTTACCATCGCGTTACTCTTAGCAATCCCCTGCAACCGTGGCGTAAAATTTTTTTTCGAGTTAGGCAACATCTGCATATAACTAAACAACCTcaca                                                                                                                                   >  1:3423394/1‑150 (MQ=255)
              aaCGGGCCAGGAGGAACCTCTTTCTGATAAACCACAAAACCATTCTGTTCAATAGTTACCAGCGCGTTACTCTGAGCAATCCCCTGCACCCGTGGCGTAAAATTTTGTTTCGAGTTAGGCAACATCTGCATATAACGAAACAACCGcaca                                                                                                                                   >  2:1571885/1‑150 (MQ=255)
                 gggCCAGGAGGAACCTCTTTCTGATAAACCACAAAACCATTCTGTTCAATAGTTACCAGCGCGTTACTCTGAGCAATCCCCTGCACCCGTGGCGTAAAATTTTGTTTCGAGTTAGGCAACATCTGCATATAACGAAACAACCGCACACct                                                                                                                                <  2:3026883/150‑1 (MQ=255)
                   gCCAGGAGGAACCTCTTTCTGATAAACCACAAAACCATTCTGTTCAATAGTTACCAGCGCGTTACTCTGAGCAATCCCCTGCACCCGTGGCGTAAAATTTTGTTTCGAGTTAGGCAACATCTGCATATAACGAAACAACCGCACACctct                                                                                                                              >  1:3612824/1‑150 (MQ=255)
                   gCCAGGAGGAACCTCTTTCTGATAAACCACAAAACCATTCTGTTCAATAGTTACCAGCGCGTTACTCTGAGCAATCCCCTGCACCCGTGGCGTAAAATTTTGTTTCGAGTTAGGCAACATCTGCATATAACGAAACAACCGCACACctct                                                                                                                              <  2:806297/150‑1 (MQ=255)
                    ccAGGAGGAACCTCTTTCTGATAAACCACAAAACCATTCTGTTCAATAGTTACCAGCGCGTTACTCTGAGCAATCCCCTGCACCCGTGGCGTAAAATTTTGTTTCGAGTTAGGCAACATCTGCATATAACGAAACAACCGCACACCTCTg                                                                                                                             >  2:2403316/1‑150 (MQ=255)
                    ccAGGAGGAACCTCTTTCTGATAAACCACAAAACCATTCTGTTCAATAGTTACCAGCGCGTTACTCTGAGCAATCCCCTGCACCCGTGGCGTAAAATTTTGTTTCGAGTTAGGCAACATCTGCATATAACGAAACAACCGCACACCTCTg                                                                                                                             >  2:3176087/1‑150 (MQ=255)
                     cAGGAGGAACCTCTTTCTGATAAACCACAAAACCATTCTGTTCAATAGTTACCAGCGCGTTACTCTGAGCAATCCCCTGCACCCGTGGCGTAAAATTTTGTTTCGAGTTAGGCAACATCTGCATATAACGAAACAACCGCACACCTCTGa                                                                                                                            >  1:1730768/1‑150 (MQ=255)
                         aggaACCTCTTTCTGATAAACCACAAAACCATTCTGTTCAATAGTTACCAGCGCGTTACTCGGAGCAATCCCCTGCACCCGTGGCGTCCAATTTTGTTTCGAGTTAGGCACCATCGGCATATAACGAAACAACCGCACGCCTCTGAcgcg                                                                                                                        >  2:3252829/1‑150 (MQ=255)
                          ggaACCTCTTTCTGATAAACCACAAAACCATTCTGTTCAATAGTTACCAGCGCGTTACTCTGAGCAATCCCCTGCACCCGTGGCGTAAAATTTTGTTTCGAGTTAGGCAACATCTGCATATAACGAAACAACCGCACACCTCTGAAGCGa                                                                                                                       >  2:127258/1‑150 (MQ=255)
                             aCCTCTTTCTGATAAACCACAAAACCATTCTGTTCAATAGTTACCAGCGCGTTACTCTGAGCAATCCCCTGCACCCGTGGCGTAAAATTTTGTTTCGAGTTAGGCAACATCTGCATATAACGAAACAACCGCACACCTCTGAAGCGAACa                                                                                                                    >  1:210548/1‑150 (MQ=255)
                             aCCTCTTTCTGATAAACCACAAAACCATTCTGTTCAATAGTTACCAGCGCGTTACTCTGAGCAATCCCCTGCACCCGTGGCGTAAAATTTTGTTTCGAGTTAGGCAACATCTGCATATAACGAAACAACCGCACACCTCTGAAGCGAACa                                                                                                                    >  2:3581755/1‑150 (MQ=255)
                                  tttCTGATAAACCACAAAACCATTCTGTTCAATAGTTACCAGCGCGTTACTCTGAGCAATCCCCTGCACCCGTGGCGTAAAATTTTGTTTCGAGTTAGGCAACATCTGCATATAACGAAACAACCGCACACCTCTGAAGCGAACAGAATc                                                                                                               <  1:3739522/150‑1 (MQ=255)
                                        aTAAACCACAAAACCATTCTGTTCAATAGTTACCAGCGCGTTACTCTGAGCAATCCCCTGCACCCGTGGCGTAAAATTTTGTTTCGAGTTAGGCAACATCTGCATATAACGAAACAACCGCACACCTCTGAAGCGAACAGAATCAAAAat                                                                                                         >  1:2482266/1‑150 (MQ=255)
                                        aTAAACCACAAAACCATTCTGTTCAATAGTTACCAGCGCGTTACTCTGAGCAATCCCCTGCACCCGTGGCGTAAAATTTTGTTTCGAGTTAGGCAACATCTGCATATAACGAAACAACCGCACACCTCTGAAGCGAACAGAATCAAAAat                                                                                                         >  1:3573741/1‑150 (MQ=255)
                                        aTAAACCACAAAACCATTCTGTTCAATAGTTACCAGCGCGTTACTCTGAGCAATCCCCTGCACCCGTGGCGTAAAATTTTGTTTCGAGTTAGGCAACATCTGCATATAACGAAACAACCGCACACCTCTGAAGCGAACAGAATCAAAAat                                                                                                         >  1:861477/1‑150 (MQ=255)
                                         tAAACCACAAAACCATTCTGTTCAATAGTTACCAGCGCGTTACTCTGAGCAATCCCCTGCACCCGTGGCGTAAAATTTTGTTTCGAGTTAGGCAACATCTGCATATAACGAAACAACCGCACACCTCTGAAGCGAACAGAATCAAAAata                                                                                                        >  2:1436668/1‑150 (MQ=255)
                                           aaCCACAAAACCATTCTGTTCAATAGTTACCAGCGCGTTACTCTGAGCAATCCCCTGCACCCGTGGCGTAAAATTTTGTTTCGAGTTAGGCAACATCTGCATATAACGAAACAACCGCACACCTCTGAAGCGAACAGAATCAAAAatata                                                                                                      >  2:2880629/1‑149 (MQ=255)
                                           aaCCACAAAACCATTCTGTTCAATAGTTACCAGCGCGTTACTCTGAGCAATCCCCTGCACCCGTGGCGTAAAATTTTGTTTCGAGTTAGGCAACATCTGCATATAACGAAACAACCGCACACCTCTGAAGCGAACAGAATCAAAAATATc                                                                                                      >  2:1052199/1‑150 (MQ=255)
                                           aaCCACAAAACCATTCTGTTCAATAGTTACCAGCGCGTTACTCTGAGCAATCCCCTGCACCCGTGGCGTAAAATTTTGTTTCGAGTTAGGCAACATCTGCATATAACGAAACAACCGCACACCTCTGAAGCGAACAGAATCAAAAATATc                                                                                                      <  1:2985599/150‑1 (MQ=255)
                                           aaCCACAAAACCATTCTGTTCAATAGTTACCAGCGCGTTACTCTGAGCAATCCCCTGCACCCGTGGCGTAAAATTTTGTTTCGAGTTAGGCAACATCTGCATATAACGAAACAACCGCACACCTCTGAAGCGAACAGAATCAAAAATATc                                                                                                      <  2:848334/150‑1 (MQ=255)
                                            aCCACAAAACCATTCTGTTCAATAGTTACCAGCGCGTTACTCTGAGCAATCCCCTGCACCCGTGGCGTAAAATTTTGTTTCGAGTTAGGCAACATCTGCATATAACGAAACAACCGCACACCTCTGAAGCGAACAGAATCAAAAATATCg                                                                                                     >  2:3143602/1‑150 (MQ=255)
                                              cacaAAACCATTCTGTTCAATAGTTACCAGCGCGTTACTCTGAGCAATCCCCTGCACCCGTGGCGTAAAATTTTGTTTCGAGTTAGGCAACATCTGCATATAACGAAACAACCGCACACCTCTGAAGCGAACAGAATCAAAAATATCGCt                                                                                                   >  2:603672/1‑150 (MQ=255)
                                               acaAAACCATTCTGTTCAATAGTTACCAGCGCGTTACTCTGAGCAATCCCCTGCACCCGTGGCGTAAAATTTTGTTTCGAGTTAGGCAACATCTGCATATAACGAAACAACCGCACACCTCTGAAGCGAACATAAACAAAAATAACGCtt                                                                                                  >  1:3482240/1‑150 (MQ=255)
                                                  aaaCTATTCTGTTCAATAGTTACCAGCGCGTTACTCTGAGCAATCCCCTGCACCCGTGGCGTAAAATTTTGTTTCGAGTTAGGCAACATCTGCATATAACGATACAACCGCACACCTCTGAAGCGAACAGAATCAAAAATATCGCTTGAt                                                                                               <  2:2809087/150‑1 (MQ=255)
                                                  aaaCCATTCTGTTCAATAGTTACCAGCGCGTTACTCTGAGCAATCCCCTGCACCCGTGGCGTAAAATTTTGTTTCGAGTTAGGCAACATCTGCATATAACGAAACAACCGCACACCTCTGAAGCGAACAGAATCAAAAATATCGCTTGAt                                                                                               <  1:672527/150‑1 (MQ=255)
                                                  aaaCCATTCTGTTCAATAGTTACCAGCGCGTTACTCTGAGCAATCCCCTGCACCCGTGGCGTAAAATTTTGTTTCGAGTTAGGCAACATCTGCATATAACGAAACAACCGCACACCTCTGAAGCGAACAGAATCAAAAATATCGCTTGAt                                                                                               >  1:1737851/1‑150 (MQ=255)
                                                  aaaCCATTCTGTTCAATAGTTACCAGCGCGTTACTCTGAGCAATCCCCTGCACCCGTGGCGTAAAATTTTGTTTCGAGTTAGGCAACATCTGCATATAACGAAACAACCGCACACCTCTGAAGCGAACAGAATCAAAAATATCGCTTGAt                                                                                               >  1:1040571/1‑150 (MQ=255)
                                                     ccATTCTGTTCAATAGTTACCAGCGCGTTACTCTGAGCAATCCCCTGCACCCGTGGCGTAAAATTTTGTTTCGAGTTAGGCAACATCTGCATATAACGAAACAACCGCACACCTCTGAAGCGAACAGAATCAAAAATATCGCTTGAtgtg                                                                                            >  1:355282/1‑150 (MQ=255)
                                                     ccATTCTGTTCAATAGTTACCAGCGCGTTACTCTGAGCAATCCCCTGCACCCGTGGCGTAAAATTTTGTTTCGAGTTAGGCAACATCTGCATATAACGAAACAACCGCACACCTCTGAAGCGAACAGAATCAAAAATATCGCTTGAtgtg                                                                                            <  2:1251145/150‑1 (MQ=255)
                                                     ccATTCTGTTCAATAGTTACCAGCGCGTTACTCTGAGCAATCCCCTGCACCCGTGGCGTAAAATTTTGTTTCGAGTTAGGCAACATCTGCATATAACGAAACAACCGCACACCTCTGAAGCGAACAGAATCAAAAATATCGCTTGAtgtg                                                                                            <  1:3689342/150‑1 (MQ=255)
                                                        ttCTGTTCAATAGTTACCAGCGCGTTACTCTGAGCAATCCCCTGCACCCGTGGCGTAAAATTTTGTTTCGAGTTAGGCAACATCTGCATATAACGAAACAACCGCACACCTCTGAAGCGAACAGAATCAAAAATATCGCTTGATGTGTAc                                                                                         >  1:3085626/1‑150 (MQ=255)
                                                           tGTTCAATAGTTACCAGCGCGTTACTCTGAGCAATCGCCTGCACCCGTGGCGTAAAATTTTGTTTCGAGTTAGGCAACATCTGCATATAACGAAACAACCGCACACCTCTGAAGCGAACAGAATCAAAAATATCGCTTGATGTGTACata                                                                                      >  1:1676319/1‑150 (MQ=255)
                                                            gTTCAATAGTTACCAGCGCGTTACTCTGAGCAATCCCCTGCACCCGTGGCGTAAAATTTTGTTTCGAGTTAGGCAACATCTGCATATAACGAAACAACCGCACACCTCTGAAGCGAACAGAATCAAAAATATCGCTTGATGTGTACatat                                                                                     <  2:453726/150‑1 (MQ=255)
                                                            gTTCAATAGTTACCAGCGCGTTACTCTGAGCAATCCCCTGCACCCGTGGCGTAAAATTTTGTTTCGAGTTAGGCAACATCTGCATATAACGAAACAACCGCACACCTCTGAAGCGAACAGAATCAAAAATATCGCTTGATGTGTACatat                                                                                     >  2:1029500/1‑150 (MQ=255)
                                                            gTTCAATAGTTACCAGCGCGTTACTCTGAGCAATCCCCTGCACCCGTGGCGTAAAATTTTGTTTCGAGTTAGGCAACATCTGCATATAACGAAACAACCGCACACCTCTGAAGCGAACAGAATCAAAAATATCGCTTGATGTGTACatat                                                                                     >  2:975232/1‑150 (MQ=255)
                                                               cAATAGTTACCAGCGCGTTACTCTGAGCAATCCCCTGCACCCGTGGCGTAAAATTTTGTTTCGAGTTAGGCAACATCTGCATATAACGAAACAACCGCACACCTCTGAAGCGAACAGAATCAAAAATATCGCTTGATGTGTACATATCAc                                                                                  <  1:3065608/150‑1 (MQ=255)
                                                                 aTAGTTACCAGCGCGTTACTCTGAGCAATCCCCTGCACCCGTGGCGTAAAATTTTGTTTCGAGTTAGGCAACATCTGCATATAACGAAACAACCGCACACCTCTGAAGCGAACAGAATCAAAAATATCGCTTGATGTGTACATATCAccc                                                                                >  1:3673663/1‑150 (MQ=255)
                                                                  tAGTTACCAGCGCGTTACTCTGAGCAATCCCCTGCACCCGTGGCGTAAAATTTTGTTTCGAGTTAGGCAACATCTGCATATAACGAAACAACCGCACACCTCTGAAGCGAACAGAATCAAAAATATCGCTTGATGTGTACATATCACCCa                                                                               >  2:641405/1‑150 (MQ=255)
                                                                   aGTTACCAGCGCGTTACTCTGAGCAATCCCCTGCACCCGTGGCGTAAAATTTTGTTTCGAGTTAGGCAACATCTGCATATAACGAAACAACCGCACACCTCTGAAGCGAACAGAATCAAAAATATCGCTTGATGTGTACATATCACCCAc                                                                              >  2:3553403/1‑150 (MQ=255)
                                                                         cAGCGCGTTACTCTGAGCAATCCCCTGCACCCGTGGCGTAAAATTTTGTTTCGAGTTAGGCAACATCTGCATATAACGAAACAACCGCACACCTCTGAAGCGAACAGAATCAAAAATATCGCTTGATGTGTACATATCACCCACGCGAAg                                                                        >  2:3768746/1‑150 (MQ=255)
                                                                         cAGCGCGTTACTCTGAGCAATCCCCTGCACCCGTGGCGTAAAATTTTGTTTCGAGTTAGGCAACATCTGCATATAACGAAACAACCGCACACCTCTGAAGCGAACAGAATCAAAAATATCGCTTGATGTGTACATATCACCCACGCGAAg                                                                        <  2:517040/150‑1 (MQ=255)
                                                                          aGCGCGTTACTCTGAGCAATCCCCTGCACCCGTGGCGTAAAATTTTGTTTCGAGTTAGGCAACATCTGCATATAACGAAACAACCGCACACCTCTGAAGCGAACAGAATCAAAAATATCGCTTGATGTGTACATATCACCCACGCGAAGc                                                                       <  2:216037/150‑1 (MQ=255)
                                                                          aGCGCGTTACTCTGAGCAATCCCCTGCACCCGAGGCGTAACATTTTGTTTCGAGTTAGGCAACATCTGCATATAACGAAACAACCGCACACCTCTGAAGCGAACAGAATCAAAAATAACGCTTGATGTGTACATATCACCCACGCGAAGc                                                                       <  1:726391/150‑1 (MQ=255)
                                                                           gcgcGTTACTCTGAGCAATCCCCTGCACCCGTGGCGTAAAATTTTGTTTCGAGTTAGGCAACATCTGCATATAACGAAACAACCGCACACCTCTGAAGCGAACAGAATCAAAAATATCGCTTGATGTGTACATATCACCCACGCGAAGCg                                                                      <  2:1956384/150‑1 (MQ=255)
                                                                              cgTTACTCTGAGCAATCCCCTGCACCCGTGGCGTAAAATTTTGTTTCGAGTTAGGCAACATCTGCATATAACGAAACAACCGCACACCTCTGAAGCGAACAGAATCAAAAATATCGCTTGATGTGTACATATCACCCACGCGAAGCGTGc                                                                   >  2:2661195/1‑150 (MQ=255)
                                                                              cgTTACTCTGAGCAATCCCCTGCACCCGTGGCGTAAAATTTTGTTTCGAGTTAGGCAACATCTGCATATAACGAAACAACCGCACACCTCTGAAGCGAACAGAATCAAAAATATCGCTTGATGTGTACATATCACCCACGCGAAGCGTGc                                                                   >  2:707329/1‑150 (MQ=255)
                                                                                ttACTCTGAGCAATCCCCTGCACCCGTGGCGTAAAATTTTGTTTCGAGTTAGGCAACATCTGCATATAACGAAACAACCGCACACCTCTGAAGCGAACAGAATCAAAAATATCGCTTGATGTGTACATATCACCCACGCGAAGCGTGCCg                                                                 >  2:1865552/1‑150 (MQ=255)
                                                                                 tACTCTGAGCAATCCCCTGCACCCGTGGCGTAAAATTTTGTTTCGAGTTAGGCAACATCTGCATATAACGAAACAACCGCACACCTCTGAAGCGAACAGAATCAAAAATATCGCTTGATGTGTACATATCACCCACGCGAAGCGTGCCga                                                                <  1:62281/150‑1 (MQ=255)
                                                                                  aCTCTGAGCAATCCCCTGCACCCGTGGCGTAAAATTTTGTTTCGAGTTAGGCAACATCTGCATATAACGAAACAACCGCACACCTCTGAAGCGAACAGAATCAAAAATATCGCTTGATGTGTACATATCACCCACGCGAAGCGTGCCgag                                                               >  1:2670964/1‑150 (MQ=255)
                                                                                  aCTCTGAGCAATCCCCTGCACCCGTGGCGTAAAATTTTGTTTCGAGTTAGGCAACATCTGCATATAACGAAACAACCGCACACCTCTGAAGCGAACAGAATCAAAAATATCGCTTGATGTGTACATATCACCCACGCGAAGCGTGCCgag                                                               >  1:404203/1‑150 (MQ=255)
                                                                                  aCTCTGAGCAATCCCCTGCACCCGTGGCGTAAAATTTTGTTTCGAGTTAGGCAACATCTGCATATAACGAAACAACCGCACACCTCTGAAGCGAACAGAATCAAAAATATCGCTTGATGTGTACATATCACCCACGCGAAGCGTGCCgag                                                               >  1:2670615/1‑150 (MQ=255)
                                                                                    tctGAGCAATCCCCTGCACCCGTGGCGTAAAATTTTGTTTCGAGTTAGGCAACATCTGCATATAACGAAACAACCGCACACCTCTGAAGCGAACAGAATCAAAAATATCGCTTGATGTGTACATATCACCCACGCGAAGCGTGCCGAGaa                                                             <  2:1093397/150‑1 (MQ=255)
                                                                                     ctGAGCAATCCCCTGCACCCGTGGCGTAAAATTTTGTTTCGAGTTAGGCAACATCTGCATATAACGAAACAACCGCACACCTCTGAAGCGAACAGAATCAAAAATATCGCTTGATGTGTACATATCACCCACGCGAAGCGTGCCGAGAAg                                                            >  2:248427/1‑150 (MQ=255)
                                                                                           aaTCCCCTGCACCCGTGGCGTAAAATTTTGTTTCGAGTTAGGCAACATCTGCATATAACGAAACAACCGCACACCTCTGAAGCGAACAGAATCAAAAATATCGCTTGATGTGTACATATCACCCACGCGAAGCGTGCCGAGAAGTTGGGc                                                      >  2:3006034/1‑150 (MQ=255)
                                                                                           aaTCCCCTGCACCCGTGGCGTAAAATTTTGTTTCGAGTTAGGCAACATCTGCATATAACGAAACAACCGCACACCTCTGAAGCGAACAGAATCAAAAATATCGCTTGATGTGTACATATCACCCACGCGAAGCGTGCCGAGAAGTTGGGc                                                      >  2:169118/1‑150 (MQ=255)
                                                                                            aTCCCCTGCACCCGTGGCGTAAAATTTTGTTTCGAGTTAGGCAACATCTGCATATAACGAAACAACCGCACACCTCTGAAGCGAACAGAATCAAAAATATCGCTTGATGTGTACATATCACCCACGCGAAGCGTGCCGAGAAGTTGGGCa                                                     >  2:3051411/1‑150 (MQ=255)
                                                                                               cccTGCACCCGTGGCGTAAAATTTTGTTTCGAGTTAGGCAACATCTGCATATAACGAAACAACCGCACACCTCTGAAGCGAACAGAATCAAAAATATCGCTTGATGTGTACATATCACCCACGCGAAGCGTGCCGAGAAGTTGGGCAAAt                                                  >  1:131260/1‑150 (MQ=255)
                                                                                               cccTGCACCCGTGGCGTAAAATTTTGTTTCGAGTTAGGCAACATCTGCATATAACGAAACAACCGCACACCTCTGAAGCGAACAGAATCAAAAATATCGCTTGATGTGTACATATCACCCACGCGAAGCGTGCCGAGAAGTTGGGCAAAt                                                  >  1:660789/1‑150 (MQ=255)
                                                                                                  tGCACCCGTGGCGTAAAATTTTGTTTCGAGTTAGGCAACATCTGCATATAACGAAACAACCGCACACCTCTGAAGCGAACAGAATCAAAAATATCGCTTGATGTGTACATATCACCCACGCGAAGCGTGCCGAGAAGTTGGGCAAATCCa                                               >  1:460372/1‑150 (MQ=255)
                                                                                                   gCACCCGTGGCGTAAAATTTTGTTTCGAGTTAGGCAACATCTGCATATAACGAAACAACCGCACACCTCTGAAGCGAACAGAATCAAAAATATCGCTTGATGTGTACATATCACCCACGCGAAGCGTGCCGAGAAGTTGGGCAAATCCAc                                              <  1:13679/150‑1 (MQ=255)
                                                                                                    cACCCGTGGCGTAAAATTTTGTTTCGAGTTAGGCAACATCTGCATATAACGAAACAACCGCACACCTCTGAAGCGAACAGAATCAAAAATATCGCTTGATGTGTACATATCACCCACGCGAAGCGTGCCGAGAAGTTGGGCAAATCCACg                                             >  2:1652352/1‑150 (MQ=255)
                                                                                                    cACCCGTGGCGTAAAATTTTGTTTCGAGTTAGGCAACATCTGCATATAACGAAACAACCGCACACCTCTGAAGCGAACAGAATCAAAAATATCGCTTGATGTGTACATATCACCCACGCGAAGCGTGCCGAGAAGTTGGGCAAATCCACg                                             >  2:2345177/1‑150 (MQ=255)
                                                                                                     aCCCGTGGCGTAAAATTTTGTTTCGAGTTAGGCAACATCTGCATATAACGAAACAACCGCACACCTCTGAAGCGAACAGAATCAAAAATATCGCTTGATGTGTACATATCACCCACGCGAAGCGTGCCGAGAAGTTGGGCAAATCCACGt                                            >  1:974057/1‑150 (MQ=255)
                                                                                                             cGTAAAATTTTGTTTCGAGTTAGGCAACATCTGCATATAACGAAACAACCGCACACCTCTGAAGCGAACAGAATCAAAAATATCGCTTGATGTGTACATATCACCCACGCGAAGCGTGCCGAGAAGTTGGGCAAATCCACGTTCCAGATa                                    >  2:2462195/1‑150 (MQ=255)
                                                                                                              gTAAAATTTTGTTTCGAGTTAGGCAACATCTGCATATAACGAAACAACCGCACACCTCTGAAGCGAACAGAATCAAAAATATCGCTTGATGTGTACATATCACCCACGCGAAGCGTGCCGAGAAGTTGGGCAAATCCACGTTCCAGATAc                                   >  1:943667/1‑150 (MQ=255)
                                                                                                              gTAAAATTTTGTTTCGAGTTAGGCAACATCTGCATATAACGAAACAACCGCACACCTCTGAAGCGAACAGAATCAAAAATATCGCTTGATGTGTACATATCACCCACGCGAAGCGTGCCGAGAAGTTGGGCAAATCCACGTTCCAGATAc                                   >  2:2827748/1‑150 (MQ=255)
                                                                                                               tAAAATTTTGTTTCGAGTTAGGCAACATCTGCATATAACGAAACAACCGCACACCTCTGAAGCGAACAGAATCAAAAATATCGCTTGATGTGTACATATCACCCACGCGAAGCGTGCCGAGAAGTTGGGCAAATCCACGTTCCAGATACa                                  >  1:2793724/1‑150 (MQ=255)
                                                                                                                aaaaTTTTGTTTCGAGTTAGGCAACATCTGCATATAACGAAACAACCGCACACCTCTGAAGCGAACAGAATCAAAAATATCGCTTGATGTGTACATATCACCCACGCGAAGCGTGCCGAGAAGTTGGGCAAATCCACGTTCCAGATACAg                                 <  1:2361677/150‑1 (MQ=255)
                                                                                                                aaaaTTTTGTTTCGAGTTAGGCAACATCTGCATATAACGAAACAACCGCACACCTCTGAAGCGAACAGAATCAAAAATATCGCTTGATGTGTACATATCACCCACGCGAAGCGTGCCGAGAAGTTGGGCAAATCCACGTTCCAGATACAg                                 <  1:2317017/150‑1 (MQ=255)
                                                                                                                aaaaTTTTGTTTCGAGTTAGGCAACATCTGCATATAACGAAACAACCGCACACCTCTGAAGCGAACAGAATCAAAAATATCGCTTGATGTGTACATATCACCCACGCGAAGCGTGCCGAGAAGTTGGGCAAATCCACGTTCCAGATACAg                                 <  1:1865552/150‑1 (MQ=255)
                                                                                                                   aTTTTGTTTCGAGTTAGGCAACATCTGCATATAACGAAACAACCGCACACCTCTGAAGCGAACAGAATCAAAAATATCGCTTGATGTGTACATATCACCCACGCGAAGCGTGCCGAGAAGTTGGGCAAATCCACGTTCCAGATACAGGGt                              >  1:2833128/1‑150 (MQ=255)
                                                                                                                       tGTTTCGAGTTAGGCAACATCTGCATATAACGAAACAACCGCACACCTCTGAAGCGAACAGAATCAAAAATATCGCTTGATGTGTACATATCACCCACGCGAAGCGTGCCGAGAAGTTGGGCAAATCCACGTTCCAGATACAGGGTATTg                          <  1:1571754/150‑1 (MQ=255)
                                                                                                                        gTTTCGAGTTAGGCAACATCTGCATATAACGAAACAACCGCACACCTCTGAAGCGAACAGAATCAAAAATATCGCTTGATGTGTACATATCACCCACGCGAAGCGTGCCGAGAAGTTGGGCAAATCCACGTTCCAGATACAGGGTATTGc                         <  2:1155234/150‑1 (MQ=255)
                                                                                                                         tttCGAGTTAGGCAACATCTGCATATAACGAAACAACCGCACACCTCTGAAGCGAACAGAATCAAAAATATCGCTTGATGTGTACATATCACCCACGCGAAGCGTGCCGAGAAGTTGGGCAAATCCACGTTCCAGATACAGGGTATTGCt                        <  2:147342/150‑1 (MQ=255)
                                                                                                                          ttCGAGTTAGGCAACATCTGCATATAACGAAGCAACCGCACACCTCTGAAGCGAACAGAATCAAAAATATCGCTTGATGTGTACATATCACCCACGCGAAGCGTGCCGAGAAGTTGGGCAAATCCACGTTCCAGATACAGGGTATTGCtt                       >  2:2264562/1‑150 (MQ=255)
                                                                                                                           tCGAGTTAGGCAACATCTGCATATAACGAAACAACCGCACACCTCTGAAGCGAACAGAATCAAAAATATCGCTTGATGTGTACATATCACCCACGCGAAGCGTGCCGAGAAGTTGGGCAAATCCACGTTCCAGATACAGGGTATTGCttt                      <  1:1648415/150‑1 (MQ=255)
                                                                                                                            cGAGTTAGGCAACATCTGCATATAACGAAACAACCGCACACCTCTGAAGCGAACAGAATCAAAAATATCGCTTGATGTGTACATATCACCCACGCGAAGCGTGCCGAGAAGTTGGGCAAATCCACGTTCCAGATACAGGGTATTGCtttt                     <  1:1022736/150‑1 (MQ=255)
                                                                                                                            cGAGTTAGGCAACATCTGCATATAACGAAACAACCGCACACCTCTGAAGCGAACAGAATCAAAAATATCGCTTGATGTGTACATATCACCCACGCGAAGCGTGCCGAGAAGTTGGGCAAATCCACGTTCCAGATACAGGGTATTGCtttt                     <  2:151302/150‑1 (MQ=255)
                                                                                                                            cGAGTTAGGCAACATCTGCATATAACGAAACAACCGCACACCTCTGAAGCGAACAGAATCAAAAATATCGCTTGATGTGTACATATCACCCACGCGAAGCGTGCCGAGAAGTTGGGCAAATCCACGTTCCAGATACAGGGTATTGCtttt                     <  1:2551015/150‑1 (MQ=255)
                                                                                                                            cGAGTTAGGCAACATCTGCATATAACGAAACAACCGCACACCTCTGAAGCGAACAGAATCAAAAATATCGCTTGATGTGTACATATCACCCACGCGAAGCGTGCCGAGAAGTTGGGCAAATCCACGTTCCAGATACAGGGTATTGCtttt                     <  2:1676319/150‑1 (MQ=255)
                                                                                                                            cGAGTTAGGCAACATCTGCATATAACGAAACAACCGCACACCTCTGAAGCGAACAGAATCAAAAATATCGCTTGATGTGTACATATCACCCACGCGAAGCGTGCCGAGAAGTTGGGCAAATCCACGTTCCAGATACAGGGTATTGCtttt                     >  2:297001/1‑150 (MQ=255)
                                                                                                                             gAGTTAGGCAACATCTGCATATAACGAAACAACCGCACACCTCTGAAGCGAACAGAATCAAAAATATCGCTTGATGTGTACATATCACCCACGCGAAGCGTGCCGAGAAGTTGGGCAAATCCACGTTCCAGATACAGGGTATTGCTTTTc                    <  2:3612824/150‑1 (MQ=255)
                                                                                                                             gAGTTAGGCAACATCTGCATATAACGAAACAACCGCACACCTCTGAAGCGAACAGAATCAAAAATATCGCTTGATGTGTACATATCACCCACGCGAAGCGTGCCGAGAAGTTGGGCAAATCCACGTTCCAGATACAGGGTATTGCTTTTc                    >  2:1814937/1‑150 (MQ=255)
                                                                                                                              aGTTAGGCAACATCTGCATATAACGAAACAACCGCACACCTCTGAAGCGAACAGAATCAAAAATATCGCTTGATGTGTACATATCACCCACGCGAAGCGTGCCGAGAAGTTGGGCAAATCCACGTTCCAGATACAGGGTATTGCTTTTcc                   >  1:1543731/1‑150 (MQ=255)
                                                                                                                              aGTTAGGCAACATCTGCATATAACGAAACAACCGCACACCTCTGAAGCGAACAGAATCAAAAATATCGCTTGATGTGTACATATCACCCACGCGAAGCGTGCCGAGAAGTTGGGCAAATCCACGTTCCAGATACAGGGTATTGCTTTTcc                   >  1:2869942/1‑150 (MQ=255)
                                                                                                                              aGTTAGGCAACATCTGCATATAACGAAACAACCGCACACCTCTGAAGCGAACAGAATCAAAAATATCGCTTGATGTGTACATATCACCCACGCGAAGCGTGCCGAGAAGTTGGGCAAATCCACGTTCCAGATACAGGGTATAGCTTTTcc                   >  1:746059/1‑150 (MQ=255)
                                                                                                                                 tAGGCAACATCTGCATATAACGAAACAACCGCACACCTCTGAAGCGAACAGAATCAAAAATATCGCTTGATGTGTACATATCACCCACGCGAAGCGTGCCGAGAAGTTGGGCAAATCCACGTTCCAGATACAGGGTATTGCTTTTCcaca                >  1:2929329/1‑150 (MQ=255)
                                                                                                                                      aaCATCTGCATATAACGAAACAACCGCACACCTCTGAAGCGAACAGAATCAAAAATATCGCTTGATGTGTACATATCACCCACGCGAAGCGTGCCGAGAAGTTGGGCAAATCCACGTTCCAGATACAGGGTATTGCTTTTCCACACCCCt           >  1:2041151/1‑150 (MQ=255)
                                                                                                                                        cATCTGCATATAACGAAACAACCGCACACCTCTGAAGCGAACAGAATCAAAAATATCGCTTGATGTGTACATATCACCCACGCGAAGCGTGCCGAGAAGTTGGGCAAATCCACGTTCCAGATACAGGGTATTGCTTTTCCACACCCCTgg         <  2:3083759/150‑1 (MQ=255)
                                                                                                                                        cATCTGCATATAACGAAACAACCGCACACCTCTGAAGCGAACAGAATCAAAAATATCGCTTGATGTGTACATATCACCCACGCGAAGCGTGCCGAGAAGTTGGGCAAATCCACGTTCCAGATACAGGGTATTGCTTTTCCACACCCCTgg         >  2:1623582/1‑150 (MQ=255)
                                                                                                                                         aTCTGCATATAACGAAACAACCGCACACCTCTGAAGCGAACAGAATCAAAAATATCGCTTGATGTGTACATATCACCCACGCGAAGCGTGCCGAGAAGTTGGGCAAATCCACGTTCCAGATACAGGGTATTGCTTTTCCACACCCCTGGa        >  2:363599/1‑150 (MQ=255)
                                                                                                                                              cATATAACGAAACAACCGCACACCTCTGAAGCGAACAGAATCAAAAATATCGCTTGATGTGTACATATCACCCACGCGAAGCGTGCCGAGAAGTTGGGCAAATCCACGTTCCAGATACAGGGTATTGCTTTTCCACACCCCTGGATTGtt   <  2:210548/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                 
AATCTGTAATCGCGAACGGGCCAGGAGGAACCTCTTTCTGATAAACCACAAAACCATTCTGTTCAATAGTTACCAGCGCGTTACTCTGAGCAATCCCCTGCACCCGTGGCGTAAAATTTTGTTTCGAGTTAGGCAACATCTGCATATCACGAAACAACCGCACACCTCTGAAGCGAACAGAATCAAAAATATCGCTTGATGTGTACATATCACCCACGCGAAGCGTGCCGAGAAGTTGGGCAAATCCACGTTCCAGATACAGGGTATTGCTTTTCCACACCCCTGGATTGTTA  >  CP009273/2183495‑2183787

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
AAATCTGTAATCGCGAACGGGCCAGGAGGAACCTCTTTCTGATAAACCACAAAACCATTCTGTTCAATAGTTACCAGCGCGTTACTCTGAGCAATCCCCTGCACCCGTGGCGTAAAATTTTGTTTCGAGTTAGGCAACATCTGCATATCACGAAACAACCGCACACCTCTGAAGCGAACAGAATCAAAAATATCGCTTGATGTGTACATATCACCCACGCGAAGCGTGCCGAGAAGTTGGGCAAATCCACGTTCCAGATACAGGGTATTGCTTTTCCACACCCCTGGATTGTTATT  >  CP009273/2183494‑2183789
                                                                                                                                                    |                                                                                                                                                   
AAATCTGTAATCGCGAACGGGCCAGGAGGAACCTCTTTCTGATAAACCACAAAACCATTCTGTTCAATAGTTACCAGCGCGTTACTCTGAGCAATCCCCTGCACCCGTGGCGTAAAATTTTGTTTCGAGTTAGGCAACATCTGCATATAA                                                                                                                                                    >  SRR19973061.3580933/1‑150 (MQ=60)
 AATCTGTAATCGCGAACGGGCCAGGAGGAACCTCTTTCTGATAAACCACAAAACCATTCTGTTCAATAGTTACCAGCGCGTTACTCTGAGCAATCCCCTGCACCCGTGGCGTAAAATTTTGTTTCGAGTTAGGCAACATCTGCATATAAC                                                                                                                                                   <  SRR19973061.1982689/150‑1 (MQ=60)
 AATCTGTAATCGCGAACGGGCCAGGAGGAACCTCTTTCTGATAAACCACAAAACCATTCTGTTCAATAGTTACCAGCGCGTTACTCTGAGCAATCCCCTGCACCCGTGGCGTAAAATTTTGTTTCGAGTTAGGCAACATCTGCATATAAC                                                                                                                                                   >  SRR19973061.1158166/1‑150 (MQ=60)
   TCTGTAATCGCGAACGGGCCAGGAGGAACCTCTTTCTGATAAACCACAAAACCATTCTGTTCAATAGTTACCAGCGCGTTACTCTGAGCAATCCCCTGCACCCGTGGCGTAAAATTTTGTTTCGAGTTAGGCAACATCTGCATATAACGA                                                                                                                                                 <  SRR19973061.3479299/150‑1 (MQ=60)
     TGTAATCGCGAACGGGCCAGGAGGAACCTCTTTCTGATAAACCACAAAACCATTCTGTTCAATAGTTACCAGCGCGTTACTCTGAGCAATCCCCTGCACCCGTGGCGTAAAATTTTGTTTCGAGTTAGGCAACATCTGCATATAACGAAA                                                                                                                                               >  SRR19973061.2223307/1‑150 (MQ=60)
      GTAATCGCGAACGGGCCAGGAGGAACCTCTTTCTGATAAACCACAAAACCATTCTGTTCAATAGTTACCAGCGCGTTACTCTGAGCAATCCCCTGCACCCGTGGCGTAAAATTTTGTTTCGAGTTAGGCAACATCTGCATATAACGAAAC                                                                                                                                              <  SRR19973061.1368951/150‑1 (MQ=60)
        AATCGCGAACGGGCCAGGAGGAACCTCTTTCTGATAAACCACAAAACCATTCTGTTCAATAGTTACCAGCGCGTTACTCTGAGCAATCCCCTGCACCCGTGGCGTAAAATTTTGTTTCGAGTTAGGCAACATCTGCATATAACGAAACAA                                                                                                                                            >  SRR19973061.1989274/1‑150 (MQ=60)
        AATCGCGAACGGGCCAGGAGGAACCTCTTTCTGATAAACCACAAAACCATTCTGTTCAATAGTTACCAGCGCGTTACTCTGAGCAATCCCCTGCACCCGTGGCGTAAAATTTTGTTTCGAGTTAGGCAACATCTGCATATAACGAAACAA                                                                                                                                            >  SRR19973061.3346988/1‑150 (MQ=60)
          TCGCGAACGGGCCAGGAGGAACCTCTTTCTGATAAACCACAAAACCATTCTGTTCAATAGTTACCAGCGCGTTACTCTGAGCAATCCCCTGCACCCGTGGCGTAAAATTTTGTTTCGAGTTAGGCAACATCTGCATATAACGAAACAACC                                                                                                                                          <  SRR19973061.1554124/150‑1 (MQ=60)
          TCGCGAACGGGCCAGGAGGAACCTCTTTCTGATAAACCACAAAACCATTCTGTTCAATAGTTACCAGCGCGTTACTCTGAGCAATCCCCTGCACCCGTGGCGTAAAATTTTGTTTCGAGTTAGGCAACATCTGCATATAACGAAACAACC                                                                                                                                          <  SRR19973061.1554300/150‑1 (MQ=60)
            GCGAACGGGCCAGGAGGAACCTCTTTCTGATAAACCACAAAACCATTCTGTTCAATAGTTACCAGCGCGTTACTCTGAGCAATCCCCTGCACCCGTGGCGTAAAATTTTGTTTCGAGTTAGGCAACATCTGCATATAACGAAACAACCGC                                                                                                                                        <  SRR19973061.707839/150‑1 (MQ=60)
             CGAACGGGCCAGGAGGAACCTCTTTCTGATAAACCACAAAACCATTCTGTTCAATAGTTACCAGCGCGTTACTCTGAGCAATCCCCTGCACCCGTGGCGTAAAATTTTGTTTCGAGTTAGGCAACATCTGCATATAACGAAACAACCGCA                                                                                                                                       >  SRR19973061.1800370/1‑150 (MQ=60)
             CGAACGGGCCAGGAGGAACCTCTTTCTGATAAACCACAAAACCATTCTGTTCAATAGTTACCAGCGCGTTACTCTGAGCAATCCCCTGCACCCGTGGCGTAAAATTTTGTTTCGAGTTAGGCAACATCTGCATATAACGAAACAACCGCA                                                                                                                                       >  SRR19973061.1808247/1‑150 (MQ=60)
             CGAACGGGCCAGGAGGAACCTCTTTCTGATAAACCACAAAACCATTCTGTTCAATAGTTACCAGCGCGTTACTCTGAGCAATCCCCTGCACCCGTGGCGTAAAATTTTGTTTCGAGTTAGGCAACATCTGCATATAACGAAACAACCGCA                                                                                                                                       >  SRR19973061.3091708/1‑150 (MQ=60)
              GAACGGGCCAGGAGGAACCTCTTTCTGATAAACCACAAAACCATTCTGTTCAATAGTTACCAGCGCGTTACTCTGAGCAATCCCCTGCACCCGTGGCGTAAAATTTTGTTTCGAGTTAGGCAACATCTGCATATAACGAAACAACCGCAC                                                                                                                                      >  SRR19973061.1108370/1‑150 (MQ=60)
              GAACGGGCCAGGAGGAACCTCTTTCTGATAAACCACAAAACCATTCTGTTCAATAGTTACCAGCGCGTTACTCTGAGCAATCCCCTGCACCCGTGGCGTAAAATTTTGTTTCGAGTTAGGCAACATCTGCATATAACGAAACAACCGCAC                                                                                                                                      >  SRR19973061.2855236/1‑150 (MQ=60)
               AACGGGCCAGGAGGAACCTCTTTCTGATAAACCACAAAACCATTCTGTTCAATAGTTACCATCGCGTTACTCTTAGCAATCCCCTGCAACCGTGGCGTAAAATTTTTTTTCGAGTTAGGCAACATCTGCATATAACTAAACAACCTCACA                                                                                                                                     >  SRR19973061.3432179/1‑150 (MQ=60)
               AACGGGCCAGGAGGAACCTCTTTCTGATAAACCACAAAACCATTCTGTTCAATAGTTACCAGCGCGTTACTCTGAGCAATCCCCTGCACCCGTGGCGTAAAATTTTGTTTCGAGTTAGGCAACATCTGCATATAACGAAACAACCGCACA                                                                                                                                     <  SRR19973061.3036947/150‑1 (MQ=60)
               AACGGGCCAGGAGGAACCTCTTTCTGATAAACCACAAAACCATTCTGTTCAATAGTTACCAGCGCGTTACTCTGAGCAATCCCCTGCACCCGTGGCGTAAAATTTTGTTTCGAGTTAGGCAACATCTGCATATAACGAAACAACCGCACA                                                                                                                                     <  SRR19973061.3782986/150‑1 (MQ=60)
                    GCCAGGAGGAACCTCTTTCTGATAAACCACAAAACCATTCTGTTCAATAGTTACCAGCGCGTTACTCTGAGCAATCCCCTGCACCCGTGGCGTAAAATTTTGTTTCGAGTTAGGCAACATCTGCATATAACGAAACAACCGCACACCTCT                                                                                                                                >  SRR19973061.3622066/1‑150 (MQ=60)
                      CAGGAGGAACCTCTTTCTGATAAACCACAAAACCATTCTGTTCAATAGTTACCAGCGCGTTACTCTGAGCAATCCCCTGCACCCGTGGCGTAAAATTTTGTTTCGAGTTAGGCAACATCTGCATATAACGAAACAACCGCACACCTCTGA                                                                                                                              <  SRR19973061.1450075/150‑1 (MQ=60)
                      CAGGAGGAACCTCTTTCTGATAAACCACAAAACCATTCTGTTCAATAGTTACCAGCGCGTTACTCTGAGCAATCCCCTGCACCCGTGGCGTAAAATTTTGTTTCGAGTTAGGCAACATCTGCATATAACGAAACAACCGCACACCTCTGA                                                                                                                              >  SRR19973061.1734226/1‑150 (MQ=60)
                      CAGGAGGAACCTCTTTCTGATAAACCACAAAACCATTCTGTTCAATAGTTACCAGCGCGTTACTCTGAGCAATCCCCTGCACCCGTGGCGTAAAATTTTGTTTCGAGTTAGGCAACATCTGCATATAACGAAACAACCGCACACCTCTGA                                                                                                                              >  SRR19973061.1735116/1‑150 (MQ=60)
                       AGGAGGAACCTCTTTCTGATAAACCACAAAACCATTCTGTTCAATAGTTACCAGCGCGTTACTCTGAGCAATCCCCTGCACCCGTGGCGTAAAATTTTGTTTCGAGTTAGGCAACATCTGCATATAACGAAACAACCGCACACCTCTGAA                                                                                                                             >  SRR19973061.850548/1‑150 (MQ=60)
                        GGAGGAACCTCTTTCTGATAAACCACAAAACCATTCTGTTCAATAGTTACCAGCGCGTTACTCTGAGCAATCCCCTGCACCCGTGGCGTAAAATTTTGTTTCGAGTTAGGCAACATCTGCATATAACGAAACAACCGCACACCTCTGAAG                                                                                                                            <  SRR19973061.2091557/150‑1 (MQ=60)
                        GGAGGAACCTCTTTCTGATAAACCACAAAACCATTCTGTTCAATAGTTACCAGCGCGTTACTCTGAGCAATCCCCTGCACCCGTGGCGTAAAATTTTGTTTCGAGTTAGGCAACATCTGCATATAACGAAACAACCGCACACCTCTGAAG                                                                                                                            <  SRR19973061.176029/150‑1 (MQ=60)
                             AACCTCTTTCTGATAAACCAAATAACCATTCTGTTCAATAGTTACCAGCGCTTTACTCTGAGCAATCCCCTGCACCCGTGGCGTAAAATTTTGTTTCGAGTTAGGCAACATCTGCATATAACGAAACAACCGCACACCTCTGAAGCGAAC                                                                                                                       <  SRR19973061.40827/150‑1 (MQ=60)
                              ACCTCTTTCTGATAAACCACAAAACCATTCTGTTCAATAGTTACCAGCGCGTTACTCTGAGCAATCCCCTGCACCCGTGGCGTAAAATTTTGTTTCGAGTTAGGCAACATCTGCATATAACGAAACAACCGCACACCTCTGAAGCGAACA                                                                                                                      >  SRR19973061.211178/1‑150 (MQ=60)
                              ACCTCTTTCTGATAAACCACAAAACCATTCTGTTCAATAGTTACCAGCGCGTTACTCTGAGCAATCCCCTGCACCCGTGGCGTAAAATTTTGTTTCGAGTTAGGCAACATCTGCATATAACGAAACAACCGCACACCTCTGAAGCGAACA                                                                                                                      <  SRR19973061.1027492/150‑1 (MQ=60)
                                  CTTTCTGATAAACCACAAAACCATTCTGTTCAATAGTTACCAGCGCGTTACTCTGAGCAATCCCCTGCACCCGTGGCGTAAAATTTTGTTTCGAGTTAGGCAACATCTGCATATAACGAAACAACCGCACACCTCTGAAGCGAACAGAAT                                                                                                                  <  SRR19973061.3061715/150‑1 (MQ=60)
                                   TTTCTGATAAACCACAAAACCATTCTGTTCAATAGTTACCAGCGCGTTACTCTGAGCAATCCCCTGCACCCGTGGCGTAAAATTTTGTTTCGAGTTAGGCAACATCTGCATATAACGAAACAACCGCACACCTCTGAAGCGAACAGAATC                                                                                                                 <  SRR19973061.3749096/150‑1 (MQ=60)
                                   TTTCTGATAAACCACAAAACCATTCTGTTCAATAGTTACCAGCGCGTTACTCTGAGCAATCCCCTGCACCCGTGGCGTAAAATTTTGTTTCGAGTTAGGCAACATCTGCATATAACGAAACAACCGCACACCTCTGAAGCGAACAGAATC                                                                                                                 <  SRR19973061.1287502/150‑1 (MQ=60)
                                    TTCTGATAAACCACAAAACCATTCTGTTCAATAGTTACCAGCGCGTTACTCTGAGCAATCCCCTGCACCCGTGGCGTAAAATTTTGTTTCGAGTTAGGCAACATCTGCATATAACGAAACAACCGCACACCTCTGAAGCGAACAGAATCA                                                                                                                <  SRR19973061.159055/150‑1 (MQ=60)
                                         ATAAACCACAAAACCATTCTGTTCAATAGTTACCAGCGCGTTACTCTGAGCAATCCCCTGCACCCGTGGCGTAAAATTTTGTTTCGAGTTAGGCAACATCTGCATATAACGAAACAACCGCACACCTCTGAAGCGAACAGAATCAAAAAT                                                                                                           >  SRR19973061.3582900/1‑150 (MQ=60)
                                         ATAAACCACAAAACCATTCTGTTCAATAGTTACCAGCGCGTTACTCTGAGCAATCCCCTGCACCCGTGGCGTAAAATTTTGTTTCGAGTTAGGCAACATCTGCATATAACGAAACAACCGCACACCTCTGAAGCGAACAGAATCAAAAAT                                                                                                           >  SRR19973061.2488582/1‑150 (MQ=60)
                                         ATAAACCACAAAACCATTCTGTTCAATAGTTACCAGCGCGTTACTCTGAGCAATCCCCTGCACCCGTGGCGTAAAATTTTGTTTCGAGTTAGGCAACATCTGCATATAACGAAACAACCGCACACCTCTGAAGCGAACAGAATCAAAAAT                                                                                                           >  SRR19973061.636951/1‑150 (MQ=60)
                                         ATAAACCACAAAACCATTCTGTTCAATAGTTACCAGCGCGTTACTCTGAGCAATCCCCTGCACCCGTGGCGTAAAATTTTGTTTCGAGTTAGGCAACATCTGCATATAACGAAACAACCGCACACCTCTGAAGCGAACAGAATCAAAAAT                                                                                                           >  SRR19973061.863714/1‑150 (MQ=60)
                                            AACCACAAAACCATTCTGTTCAATAGTTACCAGCGCGTTACTCTGAGCAATCCCCTGCACCCGTGGCGTAAAATTTTGTTTCGAGTTAGGCAACATCTGCATATAACGAAACAACCGCACACCTCTGAAGCGAACAGAATCAAAAATATC                                                                                                        <  SRR19973061.2993301/150‑1 (MQ=60)
                                                ACAAAACCATTCTGTTCAATAGTTACCAGCGCGTTACTCTGAGCAATCCCCTGCACCCGTGGCGTAAAATTTTGTTTCGAGTTAGGCAACATCTGCATATAACGAAACAACCGCACACCTCTGAAGCGAACATAAACAAAAATAACGCTT                                                                                                    >  SRR19973061.3491149/1‑150 (MQ=60)
                                                ACAAAACCATTCTGTTCAATAGTTACCAGCGCGTTACTCTGAGCAATCCCCTGCACCCGTGGCGTAAAATTTTGTTTCGAGTTAGGCAACATCTGCATATAACGAAACAACCGCACACCTCTGAAGCGAACAGAATCAAAAATATCGCTT                                                                                                    >  SRR19973061.1794593/1‑150 (MQ=60)
                                                 CAAAACCATTCTGTTCAATAGTTACCAGCGCGTTACTCTGAGCAATCCCCTGCACCCGTGGCGTAAAATTTTGTTTCGAGTTAGGCAACATCTGCATATAACGAAACAACCGCACACCTCTGAAGCGAACAGAATCAAAAATATCGCTTG                                                                                                   <  SRR19973061.3548391/150‑1 (MQ=60)
                                                 CAAAACCATTCTGTTCAATAGTTACCAGCGCGTTACTCTGAGCAATCCCCTGCACCCGTGGCGTAAAATTTTGTTTCGAGTTAGGCAACATCTGCATATAACGAAACAACCGCACACCTCTGAAGCGAACAGAATCAAAAATATCGCTTG                                                                                                   >  SRR19973061.3297552/1‑150 (MQ=60)
                                                   AAACCATTCTGTTCAATAGTTACCAGCGCGTTACTCTGAGCAATCCCCTGCACCCGTGGCGTAAAATTTTGTTTCGAGTTAGGCAACATCTGCATATAACGAAACAACCGCACACCTCTGAAGCGAACAGAATCAAAAATATCGCTTGAT                                                                                                 >  SRR19973061.1043236/1‑150 (MQ=60)
                                                   AAACCATTCTGTTCAATAGTTACCAGCGCGTTACTCTGAGCAATCCCCTGCACCCGTGGCGTAAAATTTTGTTTCGAGTTAGGCAACATCTGCATATAACGAAACAACCGCACACCTCTGAAGCGAACAGAATCAAAAATATCGCTTGAT                                                                                                 >  SRR19973061.156667/1‑150 (MQ=60)
                                                   AAACCATTCTGTTCAATAGTTACCAGCGCGTTACTCTGAGCAATCCCCTGCACCCGTGGCGTAAAATTTTGTTTCGAGTTAGGCAACATCTGCATATAACGAAACAACCGCACACCTCTGAAGCGAACAGAATCAAAAATATCGCTTGAT                                                                                                 <  SRR19973061.674270/150‑1 (MQ=60)
                                                   AAACCATTCTGTTCAATAGTTACCAGCGCGTTACTCTGAGCAATCCCCTGCACCCGTGGCGTAAAATTTTGTTTCGAGTTAGGCAACATCTGCATATAACGAAACAACCGCACACCTCTGAAGCGAACAGAATCAAAAATATCGCTTGAT                                                                                                 >  SRR19973061.1742222/1‑150 (MQ=60)
                                                   AAACCATTCTGTTCAATAGTTACCAGCGCGTTACTCTGAGCAATCCCCTGCACCCGTGGCGTAAAATTTTGTTTCGAGTTAGGCAACATCTGCATATAACGAAACAACCGCACACCTCTGAAGCGAACAGAATCAAAAATATCGCTTGAT                                                                                                 <  SRR19973061.674530/150‑1 (MQ=60)
                                                     ACCATTCTGTTCAATAGTTACCAGCGCGTTACTCTGAGCAATCCCCTGCACCCGTGGCGTAAAATTTTGTTTCGAGTTAGGCAACATCTGCATATAACGAAACAACCGCACACCTCTGAAGCGAACAGAATCAAAAATATCGCTTGATGT                                                                                               <  SRR19973061.338644/150‑1 (MQ=60)
                                                      CCATTCTGTTCAATAGTTACCAGCGCGTTACTCTGAGCAATCCCCTGCACCCGTGGCGTAAAATTTTGTTTCGAGTTAGGCAACATCTGCATATAACGAAACAACCGCACACCTCTGAAGCGAACAGAATCAAAAATATCGCTTGATGTG                                                                                              <  SRR19973061.3698774/150‑1 (MQ=60)
                                                      CCATTCTGTTCAATAGTTACCAGCGCGTTACTCTGAGCAATCCCCTGCACCCGTGGCGTAAAATTTTGTTTCGAGTTAGGCAACATCTGCATATAACGAAACAACCGCACACCTCTGAAGCGAACAGAATCAAAAATATCGCTTGATGTG                                                                                              >  SRR19973061.147814/1‑150 (MQ=60)
                                                      CCATTCTGTTCAATAGTTACCAGCGCGTTACTCTGAGCAATCCCCTGCACCCGTGGCGTAAAATTTTGTTTCGAGTTAGGCAACATCTGCATATAACGAAACAACCGCACACCTCTGAAGCGAACAGAATCAAAAATATCGCTTGATGTG                                                                                              >  SRR19973061.356241/1‑150 (MQ=60)
                                                        ATTCTGTTCAATAGTTACCAGCGCGTTACTCTGAGCAATCCCCTGCACCCGTGGCGTAAAATTTTGTTTCGAGTTAGGCAACATCTGCATATAACGAAACAACCGCACACCTCTGAAGCGAACAGAATCAAAAATATCGCTTGATGTGTA                                                                                            >  SRR19973061.2678061/1‑150 (MQ=60)
                                                         TTCTGTTCAATAGTTACCAGCGCGTTACTCTGAGCAATCCCCTGCACCCGTGGCGTAAAATTTTGTTTCGAGTTAGGCAACATCTGCATATAACGAAACAACCGCACACCTCTGAAGCGAACAGAATCAAAAATATCGCTTGATGTGTAC                                                                                           >  SRR19973061.3093580/1‑150 (MQ=60)
                                                           CTGTTCAATAGTTACCAGCGCGTTACTCTGAGCAATCCCCTGCACCCGGGGCGTAAAATTTTGTTTCGAGTTAGGCAACATCTGCATATAACGAAACAACCGCACACCTCTGAAGCGAACAGAATCAAAAATATCGCTTGATGTGTACAT                                                                                         <  SRR19973061.2988684/150‑1 (MQ=60)
                                                            TGTTCAATAGTTACCAGCGCGTTACTCTGAGCAATCGCCTGCACCCGTGGCGTAAAATTTTGTTTCGAGTTAGGCAACATCTGCATATAACGAAACAACCGCACACCTCTGAAGCGAACAGAATCAAAAATATCGCTTGATGTGTACATA                                                                                        >  SRR19973061.1680528/1‑150 (MQ=60)
                                                            TGTTCAATAGTTACCAGCGCGTTACTCTGAGCAATCCCCTGCACCCGTGGCGTAAAATTTTGTTTCGAGTTAGGCAACATCTGCATATAACGAAACAACCGCACACCTCTGAAGCGAACAGAATCAAAAATATCGCTTGATGTGTACATA                                                                                        >  SRR19973061.1680527/1‑150 (MQ=60)
                                                              TTCAATAGTTACCAGCGCGTTACTCTGAGCAATCCCCTGCACCCGTGGCGTAAAATTTTGTTTCGAGTTAGGCAACATCTGCATATAACGAAACAACCGCACACCTCTGAAGCGAACAGAATCAAAAATATCGCTTGATGTGTACATATC                                                                                      >  SRR19973061.85266/1‑150 (MQ=60)
                                                               TCAATAGTTACCAGCGCGTTACTCTGAGCAATCCCCTGCACCCGTGGCGTAAAATTTTGTTTCGAGTTAGGCAACATCTGCATATAACGAAACAACCGCACACCTCTGAAGCGAACAGAATCAAAAATATCGCTTGATGTGTACATATCA                                                                                     <  SRR19973061.3571484/150‑1 (MQ=60)
                                                                CAATAGTTACCAGCGCGTTACTCTGAGCAATCCCCTGCACCCGTGGCGTAAAATTTTGTTTCGAGTTAGGCAACATCTGCATATAACGAAACAACCGCACACCTCTGAAGCGAACAGAATCAAAAATATCGCTTGATGTGTACATATCAC                                                                                    <  SRR19973061.3073526/150‑1 (MQ=60)
                                                                 AATAGTTACCAGCGCGTTACTCTGAGCAATCCCCTGCACCCGTGGCGTAAAATTTTGTTTCGAGTTAGGCAACATCTGCATATAACGAAACAACCGCACACCTCTGAAGCGAACAGAATCAAAAATATCGCTTGATGTGTACATATCACC                                                                                   >  SRR19973061.3582343/1‑150 (MQ=60)
                                                                  ATAGTTACCAGCGCGTTACTCTGAGCAATCCCCTGCACCCGTGGCGTAAAATTTTGTTTCGAGTTAGGCAACATCTGCATATAACGAAACAACCGCACACCTCTGAAGCGAACAGAATCAAAAATATCGCTTGATGTGTACATATCACCC                                                                                  >  SRR19973061.3683053/1‑150 (MQ=60)
                                                                  ATAGTTACCAGCGCGTTACTCTGAGCAATCCCCTGCACCCGTGGCGTAAAATTTTGTTTCGAGTTAGGCAACATCTGCATATAACGAAACAACCGCACACCTCTGAAGCGAACAGAATCAAAAATATCGCTTGATGTGTACATATCACCC                                                                                  >  SRR19973061.3123215/1‑150 (MQ=60)
                                                                    AGTTACCAGCGCGTTACTCTGAGCAATCCCCTGCACCCGTGGCGTAAAATTTTGTTTCGAGTTAGGCAACATCTGCATATAACGAAACAACCGCACACCTCTGAAGCGAACAGAATCAAAAATATCGCTTGATGTGTACATATCACCCAC                                                                                <  SRR19973061.1316740/150‑1 (MQ=60)
                                                                    AGTTACCAGCGCGTTACTCTGAGCAATCCCCTGCACCCGTGGCGTAAAATTTTGTTTCGAGTTAGGCAACATCTGCATATAACGAAACAACCGCACACCTCTGAAGCGAACAGAATCAAAAATATCGCTTGATGTGTACATATCACCCAC                                                                                >  SRR19973061.216233/1‑150 (MQ=60)
                                                                           AGCGCGTTACTCTGAGCAATCCCCTGCACCCGAGGCGTAACATTTTGTTTCGAGTTAGGCAACATCTGCATATAACGAAACAACCGCACACCTCTGAAGCGAACAGAATCAAAAATAACGCTTGATGTGTACATATCACCCACGCGAAGC                                                                         <  SRR19973061.728290/150‑1 (MQ=60)
                                                                                  TACTCTGAGCAATCCCCTGCACCCGTGGCGTAAAATTTTGTTTCGAGTTAGGCAACATCTGCATATAACGAAACAACCGCACACCTCTGAAGCGAACAGAATCAAAAATATCGCTTGATGTGTACATATCACCCACGCGAAGCGTGCCGA                                                                  <  SRR19973061.500636/150‑1 (MQ=60)
                                                                                  TACTCTGAGCAATCCCCTGCACCCGTGGCGTAAAATTTTGTTTCGAGTTAGGCAACATCTGCATATAACGAAACAACCGCACACCTCTGAAGCGAACAGAATCAAAAATATCGCTTGATGTGTACATATCACCCACGCGAAGCGTGCCGA                                                                  <  SRR19973061.62517/150‑1 (MQ=60)
                                                                                  TACTCTGAGCAATCCCCTGCACCCGTGGCGTAAAATTTTGTTTCGAGTTAGGCAACATCTGCATATAACGAAACAACCGCACACCTCTGAAGCGAACAGAATCAAAAATATCGCTTGATGTGTACATATCACCCACGCGAAGCGTGCCGA                                                                  <  SRR19973061.2014427/150‑1 (MQ=60)
                                                                                   ACTCTGAGCAATCCCCTGCACCCGTGGCGTAAAATTTTGTTTCGAGTTAGGCAACATCTGCATATAACGAAACAACCGCACACCTCTGAAGCGAACAGAATCAAAAATATCGCTTGATGTGTACATATCACCCACGCGAAGCGTGCCGAG                                                                 >  SRR19973061.2316926/1‑150 (MQ=60)
                                                                                   ACTCTGAGCAATCCCCTGCACCCGTGGCGTAAAATTTTGTTTCGAGTTAGGCAACATCTGCATATAACGAAACAACCGCACACCTCTGAAGCGAACAGAATCAAAAATATCGCTTGATGTGTACATATCACCCACGCGAAGCGTGCCGAG                                                                 >  SRR19973061.2677836/1‑150 (MQ=60)
                                                                                   ACTCTGAGCAATCCCCTGCACCCGTGGCGTAAAATTTTGTTTCGAGTTAGGCAACATCTGCATATAACGAAACAACCGCACACCTCTGAAGCGAACAGAATCAAAAATATCGCTTGATGTGTACATATCACCCACGCGAAGCGTGCCGAG                                                                 >  SRR19973061.2677828/1‑150 (MQ=60)
                                                                                   ACTCTGAGCAATCCCCTGCACCCGTGGCGTAAAATTTTGTTTCGAGTTAGGCAACATCTGCATATAACGAAACAACCGCACACCTCTGAAGCGAACAGAATCAAAAATATCGCTTGATGTGTACATATCACCCACGCGAAGCGTGCCGAG                                                                 >  SRR19973061.2677487/1‑150 (MQ=60)
                                                                                   ACTCTGAGCAATCCCCTGCACCCGTGGCGTAAAATTTTGTTTCGAGTTAGGCAACATCTGCATATAACGAAACAACCGCACACCTCTGAAGCGAACAGAATCAAAAATATCGCTTGATGTGTACATATCACCCACGCGAAGCGTGCCGAG                                                                 >  SRR19973061.2677485/1‑150 (MQ=60)
                                                                                   ACTCTGAGCAATCCCCTGCACCCGTGGCGTAAAATTTTGTTTCGAGTTAGGCAACATCTGCATATAACGAAACAACCGCACACCTCTGAAGCGAACAGAATCAAAAATATCGCTTGATGTGTACATATCACCCACGCGAAGCGTGCCGAG                                                                 >  SRR19973061.405302/1‑150 (MQ=60)
                                                                                    CTCTGAGCAATCCCCTGCACCCGTGGCGTAAAATTTTGTTTCGAGTTAGGCAACATCTGCATATAACGAAACAACCGCACACCTCTGAAGCGAACAGAATCAAAAATATCGCTTGATGTGTACATATCACCCACGCGAAGCGTGCCGAGA                                                                >  SRR19973061.3813519/1‑150 (MQ=60)
                                                                                        GAGCAATCCCCTGCACCCGTGGCGTAAAATTTTGTTTCGAGTTAGGCAACATCTGCATATAACGAAACAACCGCACACCTCTGAAGCGAACAGAATCAAAAATATCGCTTGATGTGTACATATCACCCACGCGAAGCGTGCCGAGAAGTT                                                            <  SRR19973061.1116845/150‑1 (MQ=60)
                                                                                            AATCCCCTGCACCCGTGGCGTAAAATTTTGTTTCGAGTTAGGCAACATCTGCATATAACGAAACAACCGCACACCTCTGAAGCGAACAGAATCAAAAATATCGCTTGATGTGTACATATCACCCACGCGAAGCGTGCCGAGAAGTTGGGC                                                        <  SRR19973061.2743432/150‑1 (MQ=60)
                                                                                              TCCCCTGCACCCGTGGCGTAAAATTTTGTTTCGAGTTAGGCAACATCTGCATATAACGAAACAACCGCACACCTCTGAAGCGAACAGAATCAAAAATATCGCTTGATGTGTACATATCACCCACGCGAAGCGTGCCGAGAAGTTGGGCAA                                                      >  SRR19973061.2453471/1‑150 (MQ=60)
                                                                                              TCCCCTGCACCCGTGGCGTAAAATTTTGTTTCGAGTTAGGCAACATCTGCATATAACGAAACAACCGCACACCTCTGAAGCGAACAGAATCAAAAATATCGCTTGATGTGTACATATCACCCACGCGAAGCGTGCCGAGAAGTTGGGCAA                                                      >  SRR19973061.2453464/1‑150 (MQ=60)
                                                                                              TCCCCTGCACCCGTGGCGTAAAATTTTGTTTCGAGTTAGGCAACATCTGCATATAACGAAACAACCGCACACCTCTGAAGCGAACAGAATCAAAAATATCGCTTGATGTGTACATATCACCCACGCGAAGCGTGCCGAGAAGTTGGGCAA                                                      >  SRR19973061.1689181/1‑150 (MQ=60)
                                                                                                CCCTGCACCCGTGGCGTAAAATTTTGTTTCGAGTTAGGCAACATCTGCATATAACGAAACAACCGCACACCTCTGAAGCGAACAGAATCAAAAATATCGCTTGATGTGTACATATCACCCACGCGAAGCGTGCCGAGAAGTTGGGCAAAT                                                    >  SRR19973061.662485/1‑150 (MQ=60)
                                                                                                CCCTGCACCCGTGGCGTAAAATTTTGTTTCGAGTTAGGCAACATCTGCATATAACGAAACAACCGCACACCTCTGAAGCGAACAGAATCAAAAATATCGCTTGATGTGTACATATCACCCACGCGAAGCGTGCCGAGAAGTTGGGCAAAT                                                    >  SRR19973061.131682/1‑150 (MQ=60)
                                                                                                CCCTGCACCCGTGGCGTAAAATTTTGTTTCGAGTTAGGCAACATCTGCATATAACGAAACAACCGCACACCTCTGAAGCGAACAGAATCAAAAATATCGCTTGATGTGTACATATCACCCACGCGAAGCGTGCCGAGAAGTTGGGCAAAT                                                    >  SRR19973061.662490/1‑150 (MQ=60)
                                                                                                   TGCACCCGTGGCGTAAAATTTTGTTTCGAGTTAGGCAACATCTGCATATAACGAAACAACCGCACACCTCTGAAGCGAACAGAATCAAAAATATCGCTTGATGTGTACATATCATCCACGCGAAGCGTGCCGAGAAGTTGGGCAAATCCA                                                 >  SRR19973061.1820369/1‑150 (MQ=60)
                                                                                                   TGCACCCGTGGCGTAAAATTTTGTTTCGAGTTAGGCAACATCTGCATATAACGAAACAACCGCACACCTCTGAAGCGAACAGAATCAAAAATATCGCTTGATGTGTACATATCACCCACGCGAAGCGTGCCGAGAAGTTGGGCAAATCCA                                                 >  SRR19973061.3704523/1‑150 (MQ=60)
                                                                                                   TGCACCCGTGGCGTAAAATTTTGTTTCGAGTTAGGCAACATCTGCATATAACGAAACAACCGCACACCTCTGAAGCGAACAGAATCAAAAATATCGCTTGATGTGTACATATCACCCACGCGAAGCGTGCCGAGAAGTTGGGCAAATCCA                                                 >  SRR19973061.461622/1‑150 (MQ=60)
                                                                                                   TGCACCCGTGGCGTAAAATTTTGTTTCGAGTTAGGCAACATCTGCATATAACGAAACAACCGCACACCTCTGAAGCGAACAGAATCAAAAATATCGCTTGATGTGTACATATCACCCACGCGAAGCGTGCCGAGAAGTTGGGCAAATCCA                                                 <  SRR19973061.3558771/150‑1 (MQ=60)
                                                                                                    GCACCCGTGGCGTAAAATTTTGTTTCGAGTTAGGCAACATCTGCATATAACGAAACAACCGCACACCTCTGAAGCGAACAGAATCAAAAATATCGCTTGATGTGTACATATCACCCACGCGAAGCGTGCCGAGAAGTTGGGCAAATCCAC                                                <  SRR19973061.1929646/150‑1 (MQ=60)
                                                                                                    GCACCCGTGGCGTAAAATTTTGTTTCGAGTTAGGCAACATCTGCATATAACGAAACAACCGCACACCTCTGAAGCGAACAGAATCAAAAATATCGCTTGATGTGTACATATCACCCACGCGAAGCGTGCCGAGAAGTTGGGCAAATCCAC                                                <  SRR19973061.13735/150‑1 (MQ=60)
                                                                                                      ACCCGTGGCGTAAAATTTTGTTTCGAGTTAGGCAACATCTGCATATAACGAAACAACCGCACACCTCTGAAGCGAACAGAATCAAAAATATCGCTTGATGTGTACATATCACCCACGCGAAGCGTGCCGAGAAGTTGGGCAAATCCACGT                                              >  SRR19973061.976968/1‑150 (MQ=60)
                                                                                                      ACCCGTGGCGTAAAATTTTGTTTCGAGTTAGGCAACATCTGCATATAACGAAACAACCGCACACCTCTGAAGCGAACAGAATCAAAAATATCGCTTGATGTGTACATATCACCCACGCGAAGCGTGCCGAGAAGTTGGGCAAATCCACGT                                              >  SRR19973061.976534/1‑150 (MQ=60)
                                                                                                          GTGGCGTAAAATTTTGTTTCGAGTTAGGCAACATCTGCATATAACGAAACAACCGCACACCTCTGAAGCGAACAGAATCAAAAATATCGCTTGATGTGTACATATCACCCACGCGAAGCGTGCCGAGAAGTTGGGCAAATCCACGTTCCA                                          <  SRR19973061.2513210/150‑1 (MQ=60)
                                                                                                               GTAAAATTTTGTTTCGAGTTAGGCAACATCTGCATATAACGAAACAACCGCACACCTCTGAAGCGAACAGAATCAAAAATATCGCTTGATGTGTACATATCACCCACGCGAAGCGTGCCGAGAAGTTGGGCAAATCCACGTTCCAGATAC                                     >  SRR19973061.946119/1‑150 (MQ=60)
                                                                                                               GTAAAATTTTGTTTCGAGTTAGGCAACATCTGCATATAACGAAACAACCGCACACCTCTGAAGCGAACAGAATCAAAAATATCGCTTGATGTGTACATATCACCCACGCGAAGCGTGCCGAGAAGTTGGGCAAATCCACGTTCCAGATAC                                     >  SRR19973061.946062/1‑150 (MQ=60)
                                                                                                                TAAAATTTTGTTTCGAGTTAGGCAACATCTGCATATAACGAAACAACCGCACACCTCTGAAGCGAACAGAATCAAAAATATCGCTTGATGTGTACATATCACCCACGCGAAGCGTGCCGAGAAGTTGGGCAAATCCACGTTCCAGATACA                                    >  SRR19973061.2800901/1‑150 (MQ=60)
                                                                                                                TAAAATTTTGTTTCGAGTTAGGCAACATCTGCATATAACGAAACAACCGCACACCTCTGAAGCGAACAGAATCAAAAATATCGCTTGATGTGTACATATCACCCACGCGAAGCGAGCCGAGAAGTTGGGCAAATCCACGTTCCAGATACA                                    >  SRR19973061.2800991/1‑150 (MQ=60)
                                                                                                                 AAAATTTTGTTTCGAGTTAGGCAACATCTGCATATAACGAAACAACCGCACACCTCTGAAGCGAACAGAATCAAAAATATCGCTTGATGTGTACATATCACCCACGCGAAGCGTGCCGAGAAGTTGGGCAAATCCACGTTCCAGATACAG                                   <  SRR19973061.1870204/150‑1 (MQ=60)
                                                                                                                 AAAATTTTGTTTCGAGTTAGGCAACATCTGCATATAACGAAACAACCGCACACCTCTGAAGCGAACAGAATCAAAAATATCGCTTGATGTGTACATATCACCCACGCGAAGCGTGCCGAGAAGTTGGGCAAATCCACGTTCCAGATACAG                                   <  SRR19973061.2322851/150‑1 (MQ=60)
                                                                                                                 AAAATTTTGTTTCGAGTTAGGCAACATCTGCATATAACGAAACAACCGCACACCTCTGAAGCGAACAGAATCAAAAATATCGCTTGATGTGTACATATCACCCACGCGAAGCGTGCCGAGAAGTTGGGCAAATCCACGTTCCAGATACAG                                   <  SRR19973061.2322896/150‑1 (MQ=60)
                                                                                                                 AAAATTTTGTTTCGAGTTAGGCAACATCTGCATATAACGAAACAACCGCACACCTCTGAAGCGAACAGAATCAAAAATATCGCTTGATGTGTACATATCACCCACGCGAAGCGTGCCGAGAAGTTGGGCAAATCCACGTTCCAGATACAG                                   <  SRR19973061.2367683/150‑1 (MQ=60)
                                                                                                                  AAATTTTGTTTCGAGTTAGGCAACATCTGCATATAACGAAACAACCGCACACCTCTGAAGCGAACAGAATCAAAAATATCGCTTGATGTGTACATATCACCCACGCGAAGCGTGCCGAGAAGTTGGGCAAATCCACGTTCCAGATACAGG                                  <  SRR19973061.2361565/150‑1 (MQ=60)
                                                                                                                    ATTTTGTTTCGAGTTAGGCAACATCTGCATATAACGAAACAACCGCACACCTCTGAAGCGAACAGAATCAAAAATATCGCTTGATGTGTACATATCACCCACGCGAAGCGTGCCGAGAAGTTGGGCAAATCCACGTTCCAGATACAGGGT                                >  SRR19973061.2840926/1‑150 (MQ=60)
                                                                                                                    ATTTTGTTTCGAGTTAGGCAACATCTGCATATAACGAAACAACCGCACACCTCTGAAGCGAACAGAATCAAAAATATCGCTTGATGTGTACATATCACCCACGCGAAGCGTGCCGAGAAGTTGGGCAAATCCACGTTCCAGATACAGGGT                                >  SRR19973061.2840384/1‑150 (MQ=60)
                                                                                                                        TGTTTCGAGTTAGGCAACATCTGCATATAACGAAACAACCGCACACCTCTGAAGCGAACAGAATCAAAAATATCGCTTGATGTGTACATATCACCCACGCGAAGCGTGCCGAGAAGTTGGGCAAATCCACGTTCCAGATACAGGGTATTG                            <  SRR19973061.1575789/150‑1 (MQ=60)
                                                                                                                        TGTTTCGAGTTAGGCAACATCTGCATATAACGAAACAACCGCACACCTCTGAAGCGAACAGAATCAAAAATATCGCTTGATGTGTACATATCACCCACGCGAAGCGTGCCGAGAAGTTGGGCAAATCCACGTTCCAGATACAGGGTATTG                            <  SRR19973061.1575658/150‑1 (MQ=60)
                                                                                                                         GTTTCGAGTTAGGCAACATCTGCATATAACGAAACAACCGCACACCTCTGAAGCGAACAGAATCAAAAATATCGCTTGATGTGTACATATCACCCACGCGAAGCGTGCCGAGAAGTTGGGCAAATCCACGTTCCAGATACAGGGTATTGC                           >  SRR19973061.3351783/1‑150 (MQ=60)
                                                                                                                         GTTTCGAGTTAGGCAACATCTGCATATAACGAAACAACCGCACACCTCTGAAGCGAACAGAATCAAAAATATCGCTTGATGTGTACATATCACCCACGCGAAGCGTGCCGAGAAGTTGGGCAAATCCACGTTCCAGATACAGGGTATTGC                           >  SRR19973061.6554/1‑150 (MQ=60)
                                                                                                                            TCGAGTTAGGCAACATCTGCATATAACGAAACAACCGCACACCTCTGAAGCGAACAGAATCAAAAATATCGCTTGATGTGTACATATCACCCACGCGAAGCGTGCCGAGAAGTTGGGCAAATCCACGTTCCAGATACAGGGTATTGCTTT                        <  SRR19973061.1652556/150‑1 (MQ=60)
                                                                                                                             CGAGTTAGGCAACATCTGCATATAACGAAACAACCGCACACCTCTGAAGCGAACAGAATCAAAAATATCGCTTGATGTGTACATATCACCCACGCGAAGCGTGCCGAGAAGTTGGGCAAATCCACGTTCCAGATACAGGGTATTGCTTTT                       <  SRR19973061.2557511/150‑1 (MQ=60)
                                                                                                                             CGAGTTAGGCAACATCTGCATATAACGAAACAACCGCACACCTCTGAAGCGAACAGAATCAAAAATATCGCTTGATGTGTACATATCACCCACGCGAAGCGTGCCGAGAAGTTGGGCAAATCCACGTTCCAGATACAGGGTATTGCTTTT                       <  SRR19973061.1025349/150‑1 (MQ=60)
                                                                                                                               AGTTAGGCAACATCTGCATATAACGAAACAACCGCACACCTCTGAAGCGAACAGAATCAAAAATATCGCTTGATGTGTACATATCACCCACGCGAAGCGTGCCGAGAAGTTGGGCAAATCCACGTTCCAGATACAGGGTATTGCTTTTCC                     >  SRR19973061.1547700/1‑150 (MQ=60)
                                                                                                                               AGTTAGGCAACATCTGCATATAACGAAACAACCGCACACCTCTGAAGCGAACAGAATCAAAAATATCGCTTGATGTGTACATATCACCCACGCGAAGCGTGCCGAGAAGTTGGGCAAATCCACGTTCCAGATACAGGGTATTGCTTTTCC                     <  SRR19973061.3742127/150‑1 (MQ=60)
                                                                                                                               AGTTAGGCAACATCTGCATATAACGAAACAACCGCACACCTCTGAAGCGAACAGAATCAAAAATATCGCTTGATGTGTACATATCACCCACGCGAAGCGTGCCGAGAAGTTGGGCAAATCCACGTTCCAGATACAGGGTATTGCTTTTCC                     <  SRR19973061.915440/150‑1 (MQ=60)
                                                                                                                               AGTTAGGCAACATCTGCATATAACGAAACAACCGCACACCTCTGAAGCGAACAGAATCAAAAATATCGCTTGATGTGTACATATCACCCACGCGAAGCGTGCCGAGAAGTTGGGCAAATCCACGTTCCAGATACAGGGTATTGCTTTTCC                     <  SRR19973061.915515/150‑1 (MQ=60)
                                                                                                                               AGTTAGGCAACATCTGCATATAACGAAACAACCGCACACCTCTGAAGCGAACAGAATCAAAAATATCGCTTGATGTGTACATATCACCCACGCGAAGCGTGCCGAGAAGTTGGGCAAATCCACGTTCCAGATACAGGGTATTGCTTTTCC                     >  SRR19973061.2877299/1‑150 (MQ=60)
                                                                                                                               AGTTAGGCAACATCTGCATATAACGAAACAACCGCACACCTCTGAAGCGAACAGAATCAAAAATATCGCTTGATGTGTACATATCACCCACGCGAAGCGTGCCGAGAAGTTGGGCAAATCCACGTTCCAGATACAGGGTATTGCTTTTCC                     >  SRR19973061.1547571/1‑150 (MQ=60)
                                                                                                                               AGTTAGGCAACATCTGCATATAACGAAACAACCGCACACCTCTGAAGCGAACAGAATCAAAAATATCGCTTGATGTGTACATATCACCCACGCGAAGCGTGCCGAGAAGTTGGGCAAATCCACGTTCCAGATACAGGGTATAGCTTTTCC                     >  SRR19973061.748018/1‑150 (MQ=60)
                                                                                                                                  TAGGCAACATCTGCATATAACGAAACAACCGCACACCTCTGAAGCGAACAGAATCAAAAATATCGCTTGATGTGTACATATCACCCACGCGAAGCGTGCCGAGAAGTTGGGCAAATCCACGTTCCAGATACAGGGTATTGCTTTTCCACA                  <  SRR19973061.1736323/150‑1 (MQ=60)
                                                                                                                                  TAGGCAACATCTGCATATAACGAAACAACCGCACACCTCTGAAGCGAACAGAATCAAAAATATCGCTTGATGTGTACATATCACCCACGCGAAGCGTGCCGAGAAGTTGGGCAAATCCACGTTCCAGATACAGGGTATTGCTTTTCCACA                  >  SRR19973061.2936859/1‑150 (MQ=60)
                                                                                                                                  TAGGCAACATCTGCATATAACGAAACAACCGCACACCTCTGAAGCGAACAGAATCAAAAATATCGCTTGATGTGTACATATCACCCACGCGAAGCGTGCCGAGAAGTTGGGCAAATCCACGTTCCAGATACAGGGTATTGCTTTTCCACA                  >  SRR19973061.2936613/1‑150 (MQ=60)
                                                                                                                                    GGCAACATCTGCATATAACGAAACAACCGCACACCTCTGAAGCGAACAGAATCAAAAATATCGCTTGATGTGTACATATCACCCACGCGAAGCGTGCCGAGAAGTTGGGCAAATCCACGTTCCAGATACAGGGTATTGCTTTTCCACACC                <  SRR19973061.249129/150‑1 (MQ=60)
                                                                                                                                       AACATCTGCATATAACGAAACAACCGCACACCTCTGAAGCGAACAGAATCAAAAATATCGCTTGATGTGTACATATCACCCACGCGAAGCGTGCCGAGAAGTTGGGCAAATCCACGTTCCAGATACAGGGTATTGCTTTTCCACACCCCT             >  SRR19973061.2046283/1‑150 (MQ=60)
                                                                                                                                       AACATCTGCATATAACGAAACAACCGCACACCTCTGAAGCGAACAGAATCAAAAATATCGCTTGATGTGTACATATCACCCACGCGAAGCGTGCCGAGAAGTTGGGCAAATCCACGTTCCAGATACAGGGTATTGCTTTTCCACACCCCT             >  SRR19973061.2047713/1‑150 (MQ=60)
                                                                                                                                       AACATCTGCATATAACGAAACAACCGCACACCTCTGAAGCGAACAGAATCAAAAATATCGCTTGATGTGTACATATCACCCACGCGAAGCGTGCCGAGAAGTTGGGCAAATCCACGTTCCAGATACAGGGTATTGCTTTTCCACACCCCT             <  SRR19973061.2611835/150‑1 (MQ=60)
                                                                                                                                       AACATCTGCATATAACGAAACAACCGCACACCTCTGAAGCGAACAGAATCAAAAATATCGCTTGATGTGTACATATCACCCACGCGAAGCGTGCCGAGAAGTTGGGCAAATCCACGTTCCAGATACAGGGTATTGCTTTTCCACACCCCT             >  SRR19973061.3687267/1‑150 (MQ=60)
                                                                                                                                          ATCTGCATATAACGAAACAACCGCACACCTCTGAAGCGAACAGAATCAAAAATATCGCTTGATGTGTACATATCACCCACGCGAAGCGTGCCGAGAAGTTGGGCAAATCCACGTTCCAGATACAGGGTATTGCTTTTCCACACCCCTGGA          >  SRR19973061.2917277/1‑150 (MQ=60)
                                                                                                                                              GCATATAACGAAACAACCGCACACCTCTGAAGCGAACAGAATCAAAAATATCGCTTGATGTGTACATATCACCCACGCGAAGCGTGCCGAGAAGTTGGGCAAATCCACGTTCCAGATACAGGGTATTGCTTTTCCACACCCCTGGATTGT      <  SRR19973061.58548/150‑1 (MQ=60)
                                                                                                                                               CATATAACGAAACAACCGCACACCTCTGAAGCGAACAGAATCAAAAATATCGCTTGATGTGTACTTATCACCCACGCGAAGTGTGCCGAGAAGTTGGGCAAATCCACGTTCCAGATACAGGGTATTGCTTTTCCACACCCCTGGATTGTT     <  SRR19973061.3680497/150‑1 (MQ=60)
                                                                                                                                               CATATAACGAAACAACCGCACACCTCTGAAGCGAACAGAATCAAAAATATCGCTTGATGTGTACATATCACCCACGCGAAGCGTGCCGAGAAGTTGGGCAAATCCACGTTCCAGATACAGGGTATTGCTTTTCCACACCCCTGGATTGTT     >  SRR19973061.732533/1‑150 (MQ=60)
                                                                                                                                               CATATAACGAAACAACCGCACACCTCTGAAGCGAACAGAATCAAAAATATCGCTTGATGTGTACATATCACCCACGCGAAGCGTGCCGAGAAGTTGGGCAAATCCACGTTCCAGATACAGGGTATTGCTTTTCCACACCCCTGGATTGTT     <  SRR19973061.3680312/150‑1 (MQ=60)
                                                                                                                                                ATATAACGAAACAACCGCACACCTCTGAAGCGAACAGAATCAAAAATATCGCTTGATGTGTACATATCACCCACGCGAAGCGTGCCGAGAAGTTGGGCAAATCCACGTTCCAGATACAGGGTATTGCTTTTCCACACCCCTGGATTGTTA    >  SRR19973061.3587879/1‑150 (MQ=60)
                                                                                                                                                  ATAACGAAACAACCGCACACCTCTGAAGCGAACAGAATCAAAAATATCGCTTGATGTGTACATATCACCCACGCGAAGCGTGCCGAGAAGTTGGGCAAATCCACGTTCCAGATACAGGGTATTGCTTTTCCACACCCCTGGATTGTTATT  <  SRR19973061.2434941/150‑1 (MQ=60)
                                                                                                                                                  ATAACGAAACAACCGCACACCTCTGAAGCGAACAGAATCAAAAATATCGCTTGATGTGTACATATCACCCACGCGAAGCGTGCCGAGAAGTTGGGCAAATCCACGTTCCAGATACAGGGTATTGCTTTTCCACACCCCTGGATTGTTATT  <  SRR19973061.269491/150‑1 (MQ=60)
                                                                                                                                                    |                                                                                                                                                   
AAATCTGTAATCGCGAACGGGCCAGGAGGAACCTCTTTCTGATAAACCACAAAACCATTCTGTTCAATAGTTACCAGCGCGTTACTCTGAGCAATCCCCTGCACCCGTGGCGTAAAATTTTGTTTCGAGTTAGGCAACATCTGCATATCACGAAACAACCGCACACCTCTGAAGCGAACAGAATCAAAAATATCGCTTGATGTGTACATATCACCCACGCGAAGCGTGCCGAGAAGTTGGGCAAATCCACGTTCCAGATACAGGGTATTGCTTTTCCACACCCCTGGATTGTTATT  >  CP009273/2183494‑2183789

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑