Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F534 I1 R1 1235 230.2 7611948 95.1% 7238962 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009273 3,065,123 C→T G287S (GGT→AGT)  pgk ← phosphoglycerate kinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,065,1230CT99.5% 651.8 / NA 196G287S (GGT→AGT) pgkphosphoglycerate kinase
Reads supporting (aligned to +/- strand):  ref base C (0/1);  new base T (91/104);  total (91/105)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 196 total aligned reads displayed.

AGAACGCTTCGCTGTCTGCGATAGCGTTAGCCACGATTTCAGTACCTTTGCGGAAGTTCGGGAATTCGAACACGCCAACCGGACCGTTCCACAGAATGGTTTTCGCATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACCGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGTAGCCGGTGCAGTTTCAGAGAACTCGGTTGCTACGCGAACATCAGACGGAACCGGGATGTTGCAGGTGGTCAGCAGACGTTTAGCTTCGTCAACCA  >  CP009273/3064976‑3065269
                                                                                                                                                   |                                                                                                                                                  
agaACGCTTCGCTGTCTGCGATAGCGTTAGCCACGATTTCAGTACCTTTGCGGAAGTTCGGGAATTCGAACACGCCAACCGGACCGTTCCACAGAATGGTTTTCGCATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACTGa                                                                                                                                                  >  1:614119/1‑150 (MQ=255)
 gaACGCTTCGCTGTCTGCGATAGCGTTAGCCACGATTTCAGTACCTTTGCGGAAGTTCGGGAATTCGAACACGCCAACCGGACCGTTCCACAGATTGGTTTTCGCATTCTTCAGGATTTCTGCCAGTTCCTGAGCGGAAGCATCACTGat                                                                                                                                                 <  2:123783/150‑1 (MQ=255)
   aCGCTTCGCTGTCTGCGATAGCGTTAGCCACGATTTCAGTACCTTTGCGGAAGTTCGGGAATTCGAACACGCCAACCGGACCGTTCCACAGAATGGTTTTCGCATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACTGatat                                                                                                                                               >  2:818511/1‑150 (MQ=255)
    cGCTTCGCTGTCTGCGATAGCGTTAGCCACGATTTCAGTACCTTTGCGGAAGTTCGGGAATTCGAACACGCCAACCGGACCGTTCCACAGAATGGTTTTCGCATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACTGATATc                                                                                                                                              >  2:1348295/1‑150 (MQ=255)
     gCTTCGCTGTCTGCGATAGCGTTAGCCACGATTTCAGTACCTTTGCGGAAGTTCGGGAATTCGAACACGCCAACCGGACCGTTCCACAGAATGGTTTTCGCATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACTGATATcc                                                                                                                                             <  2:575968/150‑1 (MQ=255)
     gCTTCGCTGTCTGCGATAGCGTTAGCCACGATTTCAGTACCTTTGCGGAAGTTCGGGAATTCGAACACGCCAACCGGACCGTTCCACAGAATGGTTTTCGCATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACTGATATcc                                                                                                                                             <  2:2294869/150‑1 (MQ=255)
      cTTCGCTGTCTGCGATAGCGTTAGCCACGATTTCAGTACCTTTGCGGAAGTTCGGGGATTCGAACACGCCAACCGGACCGTTCCACAGAATGGTTTTCGCATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACTGATATCCa                                                                                                                                            >  2:6168/1‑150 (MQ=255)
       ttCGCTGTCTGCGATAGCGTTAGCCACGATTTCAGTACCTTTGCGGAAGTTCGGGAATTCGAACACGCCAACCGGACCGTTCCACAGAATGGTTTTCGCATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACTGATATCCAg                                                                                                                                           <  1:3747702/150‑1 (MQ=255)
        tCGCTGTCTGCGATAGCGTTAGCCACGATTTCAGTACCTTTGCGGAAGTTCGGGAATTCGAACACGCCAACCGGACCGTTCCACAGAATGGTTTTCGCATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACTGATATCCAgg                                                                                                                                          <  2:792170/150‑1 (MQ=255)
           cTGTCTGCGATAGCGTTAGCCACGATTTCAGTACCTTTGCGGAAGTTCGGGAATTCGAACACGCCAACCGGACCGTTCCACAGAATGGTTTTCGCATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACTGATATCCAGGATc                                                                                                                                       <  2:938020/150‑1 (MQ=255)
            tGTCTGCGATAGCGTTAGCCACGATTTCAGTACCTTTGCGGAAGTTCGGGAATTCGAACACGCCAACCGGACCGTTCCACAGAATGGTTTTCGCATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACTGATATCCAGGATCt                                                                                                                                      >  2:2849687/1‑150 (MQ=255)
            tGTCTGCGATAGCGTTAGCCACGATTTCAGTACCTTTGCGGAAGTTCGGGAATTCGAACACGCCAACCGGACCGTTCCACAGAATGGTTTTCGCATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACTGATATCCAGGATCt                                                                                                                                      <  2:162861/150‑1 (MQ=255)
             gTCTGCGATAGCGTTAGCCACGATTTCAGTACCTTTGCGGAAGTTCGGGAATTCGAACACGCCAACCGGACCGTTCCACAGAATGGTTTTCGCATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACTGATATCCAGGATCTg                                                                                                                                     <  1:945980/150‑1 (MQ=255)
             gTCTGCGATAGCGTTAGCCACGATTTCAGTACCTTTGCGGAAGTTCGGGAATTCGAACACGCCAACCGGACCGTTCCACAGAATGGTTTTCGCATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACTGATATCCAGGATCTg                                                                                                                                     >  1:2469497/1‑150 (MQ=255)
              tCTGCGATAGCGTTAGCCACGATTTCAGTACCTTTGCGGAAGTTCGGGAATTCGAACACGCCAACCGGACCGTTCCACAGAATGGTTTTCGCATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACTGATATCCAGGATCTGc                                                                                                                                    >  1:121541/1‑150 (MQ=255)
                tGCGATAGCGTTAGCCACGATTTCAGTACCTTTGCGGATGTTCGGGAATTCGAACACGCCAACCGGACCGTTCCACAGAATGGTTTTCGCATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACTGATATCCAGGATCTGCTc                                                                                                                                  <  2:2421495/150‑1 (MQ=255)
                    aTAGCGTTAGCCACGATTTCAGTACCTTTGCGGAAGTTCGGGAATTCGAACACGCCAACCGGACCGTTCCACAGAATGGTTTTCGCATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACTGATATCCAGGATCTGCTCGTCa                                                                                                                              <  2:411415/150‑1 (MQ=255)
                       gCGTTAGCCACGATTTCAGTACCTTTGCGGAAGTTCGGGAATTCGAACACGCCAACCGGACCGTTCCACAGAATGGTTTTCGCATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACTGATATCCAGGATCTGCTCGTCAGCt                                                                                                                           <  1:2656268/150‑1 (MQ=255)
                         gTTAGCCACGATTTCAGTACCTTTGCGGAAGTTCGGGAATTCGAACACGCCAACCGGACCGTTCCACAGAATGGTTTTCGCATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACTGATATCCAGGATCTGCTCGTCAGCttt                                                                                                                         <  1:1344473/150‑1 (MQ=255)
                          ttAGCCACGATTTCAGTACCTTTGCGGAAGTTCGGGAATTCGAACACGCCAACCGGACCGTTCCACAGAATGGTTTTCGCATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACTGATATCCAGGATCTGCTCGTCAGCTTTc                                                                                                                        >  1:1310412/1‑150 (MQ=255)
                            aGCCACGATTTCAGTACCTTTGCGGAAGTTCGGGAATTCGAACACGCCAACCGGACCGTTCCACAGAATGGTTTTCGCATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACTGATATCCAGGATCTGCTCGTCAGCTTTcac                                                                                                                      <  2:2348489/150‑1 (MQ=255)
                             gCCACGATTTCAGTACCTTTGCGGAAGTTCGGGAATTCGAACACGCCAACCGGACCGTTCCACAGAATGGTTTTCGCATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACTGATATCCAGGATCTGCTCGTCAGCTTTcaca                                                                                                                     >  2:2468984/1‑150 (MQ=255)
                                   aTTTCAGTACCTTTGCGGAAGTTCGGGAATTCGAACACGCCAACCGGACCGTTCCACAGAATGGTTTTCGCATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACTGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTa                                                                                                               >  1:1104237/1‑150 (MQ=255)
                                    tttCAGTACCTTTGCGGAAGTTCGGGAATTCGAACACGCCAACCGGACCGTTCCACAGAATGGTTTTCGCATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACTGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTaa                                                                                                              >  2:1132586/1‑150 (MQ=255)
                                    tttCAGTACCTTTGCGGAAGTTCGGGAATTCGAACACGCCAACCGGACCGTTCCACAGAATGGTTTTCGCATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACTGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTaa                                                                                                              >  2:671700/1‑150 (MQ=255)
                                          tACCTTTGCGGAAGTTCGGGAATTCGAACACGCCAACCGGACCGTTCCACAGAATGGTTTTCGCATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACTGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGAtt                                                                                                        <  2:2776528/150‑1 (MQ=255)
                                           aCCTTTGCGGAAGTTCGGGAATTCGAACACGCCAACCGGACCGTTCCACAGAATGGTTTTCGCATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACTGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGAttt                                                                                                       <  2:2555101/150‑1 (MQ=255)
                                            ccTTTGCGGAAGTTCGGGAATTCGAACACGCCAACCGGACCGTTCCACAGAATGGTTTTCGCATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACTGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTc                                                                                                      <  1:2404593/150‑1 (MQ=255)
                                               ttGCGGAAGTTCGGGAATTCGAACACGCCAACCGGACCGTTCCACAGAATGGTTTTCGCATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACTGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTCAgg                                                                                                   <  2:1950036/150‑1 (MQ=255)
                                                 gCGGAAGTTCGGGAATTCGAACACGCCAACCGGACCGTTCCACAGAATGGTTTTCGCATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACTGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGt                                                                                                 <  2:3670347/150‑1 (MQ=255)
                                                  cGGAAGTTCGGGAATTCGAACACGCCAACCGGACCGTTCCACAGAATGGTTTTCGCATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACTGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGTa                                                                                                <  1:1081456/150‑1 (MQ=255)
                                                  cGGAAGTTCGGGAATTCGAACACGCCAACCGGACCGTTCCACAGAATGGTTTTCGCATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACTGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGTa                                                                                                <  2:1133593/150‑1 (MQ=255)
                                                   ggAAGTTCGGGAATTCGAACACGCCAACCGGACCGTTCCACAGAATGGTTTTCGCATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACTGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGTAg                                                                                               <  1:1691852/150‑1 (MQ=255)
                                                   ggAAGTTCGGGAATTCGAACACGCCAACCGGACCGTTCCACAGAATGGTTTTCGCATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACTGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGTAg                                                                                               >  2:1304212/1‑150 (MQ=255)
                                                     aaGTTCGGGAATTCGAACACGCCAACCGGACCGTTCCACAGAATGGTTTTCGCATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACTGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGTAGcc                                                                                             <  2:2692768/150‑1 (MQ=255)
                                                      aGTTCGGGAATTCGAACACGCCAACCGGACCGTTCCACAGAATGGTTTTCGCATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACTGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGTAGCCg                                                                                            >  2:1171393/1‑150 (MQ=255)
                                                        ttCGGGAATTCGAACACGCCAACCGGACCGTTCCACAGAATGGTTTTCGCATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACTGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGTAGCCGGt                                                                                          >  2:1478663/1‑150 (MQ=255)
                                                         tCGGGAATTCGAACACGCCAACCGGACCGTTCCACAGAATGGTTTTCGCATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACTGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGTAGCCGGTg                                                                                         <  1:1631110/150‑1 (MQ=255)
                                                         tCGGGAATTCGAACACGCCAACCGGACCGTTCCACAGAATGGTTTTCGCATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACTGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGTAGCCGGTg                                                                                         >  1:89483/1‑150 (MQ=255)
                                                           gggAATTCGAACACGCCAACCGGACCGTTCCACAGAATGGTTTTCGCATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACTGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGTAGCCGGTGCa                                                                                       >  1:3571963/1‑150 (MQ=255)
                                                           gggAATTCGAACACGCCAACCGGACCGTTCCACAGAATGGTTTTCGCATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACTGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGTAGCCGGTGCa                                                                                       <  2:1390516/150‑1 (MQ=255)
                                                           gggAATTCGAACACGCCAACCGGACCGTTCCACAGAATGGTTTTCGCATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACTGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGTAGCCGGTGCa                                                                                       <  2:2920399/150‑1 (MQ=255)
                                                             gAATTCGAACACGCCAACCGGACCGTTCCACAGAATGGTTTTCGCATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACTGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGTAGCCGGTGCAGt                                                                                     >  1:668916/1‑150 (MQ=255)
                                                                  cNAACACGCCAACCGGACCGTTCCACAGAATGGTTTTCGCATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACTGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGTAGCCGGTGCAGTTTCag                                                                                >  1:1637994/1‑150 (MQ=255)
                                                                  cGAACACGCCAACCGGACCGTTCCACAGAATGGTTTTCGCATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACTGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGTAGCCGGTGCAGTTTCag                                                                                >  2:3714276/1‑150 (MQ=255)
                                                                  cGAACACGCCAACCGGACCGTTCCACAGAATGGTTTTCGCATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACTGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGTAGCCGGTGCAGTTTCag                                                                                >  2:3641631/1‑150 (MQ=255)
                                                                   gAACACGCCAACCGGACCGTTCCACAGAATGGTTTTCGCATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACTGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGTAGCCGGTGCAGTTTCaga                                                                               >  2:183781/1‑150 (MQ=255)
                                                                   gAACACGCCAACCGGACCGTTCCACAGAATGGTTTTCGCATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACTGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGTAGCCGGTGCAGTTTCaga                                                                               <  2:3073673/150‑1 (MQ=255)
                                                                    aaCACGCCAACCGGACCGTTCCACAGAATGGTTTTCGCATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACTGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGTAGCCGGTGCAGTTTCagag                                                                              <  1:3332348/150‑1 (MQ=255)
                                                                    aaCACGCCAACCGGACCGTTCCACAGAATGGTTTTCGCATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACTGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGTAGCCGGTGCAGTTTCagag                                                                              >  1:1929739/1‑150 (MQ=255)
                                                                     acacGCCAACCGGACCGTTCCACAGAATGGTTTTCGCATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACTGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGTAGCCGGTGCAGTTTCAgaga                                                                             >  1:3497699/1‑150 (MQ=255)
                                                                        cGCCAACCGGACCGTTCCACAGAATGGTTTTCGCATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACTGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGTAGCCGGTGCAGTTTCAGAGAACt                                                                          <  2:1595731/150‑1 (MQ=255)
                                                                         gCCAACCGGACCGTTCCACAGAATGGTTTTCGCATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACTGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGTAGCCGGTGCAGTTTCAGAGAACTc                                                                         >  1:3451029/1‑150 (MQ=255)
                                                                          ccAACCGGACCGTTCCACAGAATGGTTTTCGCATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACTGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGTAGCCGGTGCAGTTTCAGAGAACTCg                                                                        >  1:2896270/1‑150 (MQ=255)
                                                                           cAACCGGACCGTTCCACAGAATGGTTTTCGCATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACTGATATCCAGGATCTGCTCGTCAGCTTTCACAGCGTTAACAGATTTCAGGGTAGCCGGTGCAGTTTCAGAGAACTCgg                                                                       >  2:2106344/1‑150 (MQ=255)
                                                                              ccGGACCGTTCCACAGAATGGTTTTCGCATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACTGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGTAGCCGGTGCAGTTTCAGAGAACTCGGTTg                                                                    >  1:3222012/1‑150 (MQ=255)
                                                                               cGGACCGTTCCACAGAATGGTTTTCGCATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACTGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGTAGCCGGTGCAGTTTCAGAGAACTCGGTTGc                                                                   >  2:3397759/1‑150 (MQ=255)
                                                                                 gACCGTTCCACAGAATGGTTTTCGCATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACTGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGTAGCCGGTGCAGTTTCAGAGAACTCGGTTGCTa                                                                 >  1:218637/1‑150 (MQ=255)
                                                                                  aCCGTTCCACAGAATGGTTTTCGCATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACTGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGTAGCCGGTGCAGTTTCAGAGAACTCGGTTGCTAc                                                                >  1:885554/1‑150 (MQ=255)
                                                                                    cGTTCCACAGAATGGTTTTCGCATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACTGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGTAGCCGGTGCAGTTTCAGAGAACTCGGTTGCTAcgc                                                              >  1:3691978/1‑150 (MQ=255)
                                                                                      ttCCACAGAATGGTTTTCGCATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACTGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGTAGCCGGTGCAGTTTCAGAGAACTCGGTTGCTACGCGa                                                            >  2:207087/1‑150 (MQ=255)
                                                                                        ccACAGAATGGTTTTCGCATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACTGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGTAGCCGGTGCAGTTTCAGAGAACTCGGTTGCTACGCGAAc                                                          <  2:2040177/150‑1 (MQ=255)
                                                                                         cacaGAATGGTTTTCGCATTCGTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACTGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGTAGCCGGTGCAGTTTCAGAGAACTCGGTTGCTACGCGAACa                                                         <  2:1280800/150‑1 (MQ=255)
                                                                                            aGAATGGTTTTCGCATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACTGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGTAGCCGGTGCAGTTTCAGAGAACTCGGTTGCTACGCGAACATCa                                                      >  2:2803696/1‑150 (MQ=255)
                                                                                               aTGGTTTTCGCATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACTGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGTAGCCGGTGCAGTTTCAGAGAACTCGGTTGCTACGCGAACATCAGAc                                                   <  1:1349427/150‑1 (MQ=255)
                                                                                               aTGGTTTTCGCATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACTGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGTAGCCGGTGCAGTTTCAGAGAACTCGGTTGCTACGCGAACATCAGAc                                                   <  1:1132586/150‑1 (MQ=255)
                                                                                                 ggTTTTCGCATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACTGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGTAGCCGGTGCAGTTTCAGAGAACTCGGTTGCTACGCGAACATCAGACgg                                                 >  1:2001963/1‑150 (MQ=255)
                                                                                                   ttttCGCATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACTGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGTAGCCGGTGCAGTTTCAGAGAACTCGGTTGCTACGCGAACATCAGACGGaa                                               >  1:2134732/1‑150 (MQ=255)
                                                                                                     ttCGCATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACTGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGTAGCCGGTGCAGTTTCAGAGAACTCGGTTGATACGCGAACATCAGACGGAAcc                                             <  1:2849687/150‑1 (MQ=255)
                                                                                                      tCGCATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACTGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGTAGCCGGTGCAGTTTCAGAGAACTCGGTTGCTACGCGAACATCAGACGGACCCg                                            >  2:1764889/1‑150 (MQ=255)
                                                                                                       cGCATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACTGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGTAGCCGGTGCAGTTTCAGAGAACTCGGTTGCTACGCGAACATCAGACGGAACCgg                                           <  1:1447864/150‑1 (MQ=255)
                                                                                                        gCATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACTGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGTAGCCGGTGCAGTTTCAGAGAACTCGGTTGCTACGCGAACATCAGACGGAACCggg                                          >  1:2359941/1‑150 (MQ=255)
                                                                                                           ttcttcAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACTGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGTAGCCGGTGCAGTTTCAGAGAACTCGGTTGCTACGCGAACATCAGACGGAACCGGGATg                                       >  1:844611/1‑150 (MQ=255)
                                                                                                             cttcAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACTGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGTAGCCGGTGCAGTTTCAGAGAACTCGGTTGCTACGCGAACATCAGACGGAACCGGGATGtt                                     <  2:1024601/150‑1 (MQ=255)
                                                                                                               tcAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACTGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGTAGCCGGTGCAGTTTCAGAGAACTCGGTTGCTACGCGAACATCAGACGGAACCGGGATGTTGc                                   <  1:3011678/150‑1 (MQ=255)
                                                                                                                cAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACTGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGTAGCCGGTGCAGTTTCAGAGAACTCGGTTGCTACGCGAACATCAGACGGAACCGGGATGTTGCa                                  >  2:258173/1‑150 (MQ=255)
                                                                                                                  ggATTTCAGCCAGTTCCTGAGCGGAAGCATCACTGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGTAGCCGGTGCAGTTTCAGAGAACTCGGTTGCTACGCGAACATCAGACGGAACCGGGATGTTGCAgg                                <  2:1444420/150‑1 (MQ=255)
                                                                                                                  ggATTTCAGCCAGTTCCTGAGCGGAAGCATCACTGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGTAGCCGGTGCAGTTTCAGAGAACTCGGTTGCTACGCGAACATCAGACGGAACCGGGATGTTGCAgg                                <  1:3641631/150‑1 (MQ=255)
                                                                                                                  ggATTTCAGCCAGTTCCTGAGCGGAAGCATCACTGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGTAGCCGGTGCAGTTTCAGAGAACTCGGTTGCTACGCGAACATCAGACGGAACCGGGATGTTGCAgg                                <  1:3714276/150‑1 (MQ=255)
                                                                                                                    aTTTCAGCCAGTTCCTGAGCGGAAGCATCACTGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGTAGCCGGTGCAGTTTCAGAGAACTCGGTTGCTACGCGAACATCAGACGGAACCGGGATGTTGCAggtg                              >  2:1757118/1‑150 (MQ=255)
                                                                                                                     tttCAGCCAGTTCCTGAGCGGAAGCATCACTGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGTAGCCGGTGCAGTTTCAGAGAACTCGGTTGCTACGCGAACATCAGACGGAACCGGGATGTTGCAggtgg                             >  1:113949/1‑150 (MQ=255)
                                                                                                                         aGCCAGTTCCTGAGCGGAAGCATCACTGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGTAGCCGGTGCAGTTTCAGAGAACTCGGTTGCTACGCGAACATCAGACGGAACCGGGATGTTGCAGGTGGTcag                         >  1:2746938/1‑150 (MQ=255)
                                                                                                                           ccAGTTCCTGAGCGGAAGCATCACTGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGTAGCCGGTGCAGTTTCAGAGAACTCGGTTGCTACGCGAACATCAGACGGAACCGGGATGTTGCAGGTGGTcagca                       <  1:1478663/150‑1 (MQ=255)
                                                                                                                           ccAGTTCCTGAGCGGAAGCATCACTGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGTAGCCGGTGCAGTTTCAGAGAACTCGGTTGCTACGCGAACATCAGACGGAACCGGGATGTTGCAGGTGGTcagca                       <  1:3424871/150‑1 (MQ=255)
                                                                                                                           ccAGTTCCTGAGCGGAAGCATCACTGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGTAGCCGGTGCAGTTTCAGAGAACTCGGTTGCTACGCGAACATCAGACGGAACCGGGATGTTGCAGGTGGTcagca                       >  2:2125333/1‑150 (MQ=255)
                                                                                                                            cAGTTCCTGAGCGGAAGCATCACTGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGTAGCCGGTGCAGTTTCAGAGAACTCGGTTGCTACGCGAACATCAGACGGAACCGGGATGTTGCAGGTGGTcagcag                      >  2:3511788/1‑150 (MQ=255)
                                                                                                                             aGTTCCTGAGCGGAAGCATCACTGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGTAGCCGGTGCAGTTTCAGAGAACTCGGTTGCTACGCGAACATCAGACGGAACCGGGATGTTGCAGGTGGTCAGCAGa                     >  1:2729338/1‑150 (MQ=255)
                                                                                                                             aGTTCCTGAGCGGAAGCATCACTGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGTAGCCGGTGCAGTTTCAGAGAACTCGGTTGCTACGCGAACATCAGACGGAACCGGGATGTTGCAGGTGGTCAGCAGa                     >  2:3776688/1‑150 (MQ=255)
                                                                                                                             aGTTCCTGAGCGGAAGCATCACTGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGTAGCCGGTGCAGTTTCAGAGAACTCGGTTGCTACGCGAACATCAGACGGAACCGGGATGTTGCAGGTGGTCAGCAGa                     >  1:1397755/1‑150 (MQ=255)
                                                                                                                              gTTCCTGAGCGGAAGCATCACTGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGTAGCCGGTGCAGTTTCAGAGAACTCGGTTGCTACGCGAACATCAGACGGAACCGGGATGTTGCAGGTGGTCAGCAGAc                    <  2:3545869/150‑1 (MQ=255)
                                                                                                                                  cTGAGCGGAAGCATCACTGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGTAGCCGGTGCAGTTTCAGAGAACTCGGTTGCTACGCGAACATCAGACGGAACCGGGATGTTGCAGGTGGTCAGCAGACGttt                <  2:536722/150‑1 (MQ=255)
                                                                                                                                  cTGAGCGGAAGCATCACTGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGTAGCCGGTGCAGTTTCAGAGAACTCGGTTGCTACGCGAACATCAGACGGAACCGGGATGTTGCAGGTGGTCAGCAGACGttt                <  1:818511/150‑1 (MQ=255)
                                                                                                                                   tGAGCGGAAGCATCACTGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGTAGCCGGTGCAGTTTCAGAGAACTCGGTTGCTACGCGAACATCAGACGGAACCGGGATGTTGCAGGTGGTCAGCAGACGTTTa               >  2:3523687/1‑150 (MQ=255)
                                                                                                                                   tGAGCGGAAGCATCACTGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGTAGCCGGTGCAGTTTCAGAGAACTCGGTTGCTACGCGAACATCAGACGGAACCGGGATGTTGCAGGTGGTCAGCAGACGTTTa               >  1:3730509/1‑150 (MQ=255)
                                                                                                                                    gAGCGGAAGCATCACTGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGTAGCCGGTGCAGTTTCAGAGAACTCGGTTGCTACGCGAACATCAGACGGAACCGGGATGTTGCAGGTGGTCAGCAGACGTTTAg              <  2:3298367/150‑1 (MQ=255)
                                                                                                                                        ggAAGCATCACTGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGTAGCCGGTGCAGTTTCAGAGAACTCGGTTGCTACGCGAACATCAGACGGAACCGGGATGTTGCAGGTGGTCAGCAGACGTTTAGCTTc          <  1:576900/150‑1 (MQ=255)
                                                                                                                                        ggAAGCATCACTGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGTAGCCGGTGCAGTTTCAGAGAACTCGGTTGCTACGCGAACATCAGACGGAACCGGGATGTTGCAGGTGGTCAGCAGACGTTTAGCTTc          <  2:1929739/150‑1 (MQ=255)
                                                                                                                                         gAAGCATCACTGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGTAGCCGGTGCAGTTTCAGAGAACTCGGTTGCTACGCGAACATCAGACGGAACCGGGATGTTGCAGGTGGTCAGCAGACGTTTAGCTTCg         >  1:529627/1‑150 (MQ=255)
                                                                                                                                          aaGCATCACTGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGTAGCCGGTGCAGTTTCAGAGAACTCGGTTGCTACGCGAACATCAGACGGAACCGGGATGTTGCAGGTGGTCAGCAGACGTTTAGCTTCGt        >  1:359046/1‑150 (MQ=255)
                                                                                                                                                cACTGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGTAGCCGGTGCAGTTTCAGAGAACTCGGTTGCTACGCGAACATCAGACGGAACCGGGATGTTGCAGGTGGTCAGCAGACGTTTAGCTTCGTCAACCa  >  1:410802/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
AGAACGCTTCGCTGTCTGCGATAGCGTTAGCCACGATTTCAGTACCTTTGCGGAAGTTCGGGAATTCGAACACGCCAACCGGACCGTTCCACAGAATGGTTTTCGCATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACCGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGTAGCCGGTGCAGTTTCAGAGAACTCGGTTGCTACGCGAACATCAGACGGAACCGGGATGTTGCAGGTGGTCAGCAGACGTTTAGCTTCGTCAACCA  >  CP009273/3064976‑3065269

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GAGAACGCTTCGCTGTCTGCGATAGCGTTAGCCACGATTTCAGTACCTTTGCGGAAGTTCGGGAATTCGAACACGCCAACCGGACCGTTCCACAGAATGGTTTTCGCATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACCGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGTAGCCGGTGCAGTTTCAGAGAACTCGGTTGCTACGCGAACATCAGACGGAACCGGGATGTTGCAGGTGGTCAGCAGACGTTTAGCTTCGTCAACCAG  >  CP009273/3064975‑3065270
                                                                                                                                                    |                                                                                                                                                   
GAGAACGCTTCGCTGTCTGCGATAGCGTTAGCCACGATTTCAGTACCTTTGCGGAAGTTCGGGAATTCGAACACGCCAACCGGACCGTTCCACAGAATGGTTTTCGCATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACTG                                                                                                                                                    >  SRR19973061.2390622/1‑150 (MQ=60)
 AGAACGCTTCGCTGTCTGCGATAGCGTTAGCCACGATTTCAGTACCTTTGCGGAAGTTCGGGAATTCGAACACGCCAACCGGACCGTTCCACAGAATGGTTTTCGCATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACTGA                                                                                                                                                   >  SRR19973061.615699/1‑150 (MQ=60)
    ACGCTTCGCTGTCTGCGATAGCGTTAGCCACGATTTCAGTACCTTTGCGGAAGTTCGGGAATTCGAACACGCCAACCGGACCGGTCCACAGAATGGTTTTCGCATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACTGATAT                                                                                                                                                <  SRR19973061.2744750/150‑1 (MQ=60)
       CTTCGCTGTCTGCGATAGCGTTAGCCACGATTTCAGTACCTTTGCGGAAGTTCGGGAATTCGAACACGCCAACCGGACCGTTCCACAGAATGGTTTTCGCATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACTGATATCCA                                                                                                                                             >  SRR19973061.1136484/1‑150 (MQ=60)
        TTCGCTGTCTGCGATAGCGTTAGCCACGATTTCAGTACCTTTGCGGAAGTTCGGGAATTCGAACACGCCAACCGGACCGTTCCACAGAATGGTTTTCGCATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACTGATATCCAG                                                                                                                                            <  SRR19973061.3757292/150‑1 (MQ=60)
              GTCTGCGATAGCGTTAGCCACGATTTCAGTACCTTTGCGGAAGTTCGGGAATTCGAACACGCCAACCGGACCGTTCCACAGAATGGTTTTCGCATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACTGATCTCCAGGATCTG                                                                                                                                      >  SRR19973061.2475785/1‑150 (MQ=60)
              GTCTGCGATAGCGTTAGCCACGATTTCAGTACCTTTGCGGAAGTTCGGGAATTCGAACACGCCAACCGGACCGTTCCACAGAATGGTTTTCGCATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACTGATATCCAGGATCTG                                                                                                                                      <  SRR19973061.948375/150‑1 (MQ=60)
               TCTGCGATAGCGTTAGCCACGATTTCAGTACCTTTGCGGAAGTTCGGGAATTCGAACACGCCAACCGGACCGTTCCACAGAATGGTTTTCGCATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACTGATATCCAGGATCTGC                                                                                                                                     >  SRR19973061.121938/1‑150 (MQ=60)
               TCTGCGATAGCGTTAGCCACGATTTCAGTACCTTTGCGGAAGTTCGGGAATTCGAACACGCCAACCGGACCGTTCCACAGAATGGTTTTCGCATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACTGATATCCAGGATCTGC                                                                                                                                     >  SRR19973061.1669868/1‑150 (MQ=60)
                     ATAGCGTTAGCCACGATTTCAGTACCTTTGCGGAAGTTCGGGAATTCGAACACGCCAACCGGACCGTTCCACAGAATGGTTTTCGCATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACTGATATCCAGGATCTGCTCGTCA                                                                                                                               <  SRR19973061.537628/150‑1 (MQ=60)
                        GCGTTAGCCACGATTTCAGTACCTTTGCGGAAGTTCGGGAATTCGAACACGCCAACCGGACCGTTCCACAGAATGGTTTTCGCATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACTGATATCCAGGATCTGCTCGTCAGCT                                                                                                                            <  SRR19973061.2663092/150‑1 (MQ=60)
                          GTTAGCCACGATTTCAGTACCTTTGCGGAAGTTCGGGAATTCGAACACGCCAACCGGACCGTTCCACAGAATGGTTTTCGCATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACTGATATCCAGGATCTGCTCGTCAGCTTT                                                                                                                          <  SRR19973061.1347854/150‑1 (MQ=60)
                           TTAGCCACGATTTCAGTACCTTTGCGGAAGTTCGGGAATTCGAACACGCCAACCGGACCGTTCCACAGAATGGTTTTCGCATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACTGATATCCAGGATCTGCTCGTCAGCTTTC                                                                                                                         >  SRR19973061.1312415/1‑150 (MQ=60)
                           TTAGCCACGATTTCAGTACCTTTGCGGAAGTTCGGGAATTCGAACACGCCAACCGGACCGTTCCACAGAATGGTTTTCGCATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACTGATATCCAGGATCTGCTCGTCAGCTTTC                                                                                                                         >  SRR19973061.1313692/1‑150 (MQ=60)
                                CACGATTTCAGTACCTTTGCGGAAGTTCGGGAATTCGAACACGCCAACCGGACCGTTCCACAGAATGGTTTTCGCATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACTGATATCCAGGATCTGCTCGTCAGCTTTCACATC                                                                                                                    >  SRR19973061.495663/1‑150 (MQ=60)
                                    ATTTCAGTACCTTTGCGGAAGTTCGGGAATTCGAACACGCCAACCGGACCGTTCCACAGAATGGTTTTCGCATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACTGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTA                                                                                                                >  SRR19973061.1107061/1‑150 (MQ=60)
                                    ATTTCAGTACCTTTGCGGAAGTTCGGGAATTCGAACACGCCAACCGGACCGTTCCACAGAATGGTTTTCGCATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACTGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTA                                                                                                                <  SRR19973061.217837/150‑1 (MQ=60)
                                      TTCAGTACCTTTGCGGAAGTTCGGGAATTCGAACACGCCAACCGGACCGTTCCACAGAATGGTTTTCGCATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACTGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAAC                                                                                                              >  SRR19973061.1436351/1‑150 (MQ=60)
                                             CCTTTGCGGAAGTTCGGGAATTCGAACACGCCAACCGGACCGTTCCACAGAATGGTTTTCGCATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACTGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTC                                                                                                       <  SRR19973061.2410728/150‑1 (MQ=60)
                                             CCTTTGCGGAAGTTCGGGAATTCGAACACGCCAACCGGACCGTTCCACAGAATGGTTTTCGCATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACTGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTC                                                                                                       <  SRR19973061.3326273/150‑1 (MQ=60)
                                             CCTTTGCGGAAGTTCGGGAATTCGAACACGACAACCGGACCGTTCCACAGAATGGTTTTAGCATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACTGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTC                                                                                                       <  SRR19973061.2410674/150‑1 (MQ=60)
                                                   CGGAAGTTCGGGAATTCGAACACGCCAACCGGACCGTTCCACAGAATGGTTTTCGCATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACTGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGTA                                                                                                 <  SRR19973061.1084227/150‑1 (MQ=60)
                                                   CGGAAGTTCGGGAATTCGAACACGCCAACCGGACCGTTCCACAGAATGGTTTTCGCATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACTGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGTA                                                                                                 <  SRR19973061.3167295/150‑1 (MQ=60)
                                                   CGGAAGTTCGGGAATTCGAACACGCCAACCGGACCGTTCCACAGAATGGTTTTCGCATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACTGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGTA                                                                                                 >  SRR19973061.1901864/1‑150 (MQ=60)
                                                    GGAAGTTCGGGAATTCGAACACGCCAACCGGACCGGTCCACAGAATGGTTTTCGCATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACTGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGTAG                                                                                                <  SRR19973061.1696101/150‑1 (MQ=60)
                                                       AGTTCGGGAATTCGAACACGCCAACCGGACCGTTCCACAGAATGGTTTTCGCATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACTGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGTAGCCG                                                                                             <  SRR19973061.2416946/150‑1 (MQ=60)
                                                         TTCGGGAATTCGAACACGCCAACCGGACCGTTCCACAGAATGGTTTTCGCATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACTGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGTAGCCGGT                                                                                           >  SRR19973061.1481811/1‑150 (MQ=60)
                                                          TCGGGAATTCGAACACGCCAACCGGACCGTTCCACAGAATGGTTTTCGCATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACTGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGTAGCCGGTG                                                                                          <  SRR19973061.1635650/150‑1 (MQ=60)
                                                          TCGGGAATTCGAACACGCCAACCGGACCGTTCCACAGAATGGTTTTCGCATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACTGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGTAGCCGGTG                                                                                          >  SRR19973061.89783/1‑150 (MQ=60)
                                                          TCGGGAATTCGAACACGCCAACCGGACCGTTCCACAGAATGGTTTTCGCATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACTGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGTAGCCGGTG                                                                                          <  SRR19973061.1635189/150‑1 (MQ=60)
                                                            GGGAATTCGAACACGCCAACCGGACCGTTCCACAGAATGGTTTTCGCATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACTGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGTAGCCGGTGCA                                                                                        >  SRR19973061.3581115/1‑150 (MQ=60)
                                                            GGGAATTCGAACACGCCAACCGGACCGTTCCACAGAATGGTTTTCGCATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACTGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGTAGCCGGTGCA                                                                                        >  SRR19973061.1491963/1‑150 (MQ=60)
                                                              GAATTCGAACACGCCAACCGGACCGTTCCACAGAATGGTTTTCGCATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACTGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGTAGCCGGTGCAGT                                                                                      >  SRR19973061.821429/1‑150 (MQ=60)
                                                              GAATTCGAACACGCCAACCGGACCGTTCCACAGAATGGTTTTCGCATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACTGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGTAGCCGGTGCAGT                                                                                      >  SRR19973061.670647/1‑150 (MQ=60)
                                                                 TTCGAACACGCCAACCGGACCGTTCCACAGAATGGTTTTCGCATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACTGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGTAGCCGGTGCAGTTTC                                                                                   <  SRR19973061.1174357/150‑1 (MQ=60)
                                                                  TCGAACACGCCAACCGGACCGTTCCACAGAATGGTTTTCGCATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACTGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGTAGCCGGTGCAGTTTCA                                                                                  >  SRR19973061.2864975/1‑150 (MQ=60)
                                                                  TCGAACACGCCAACCGGACCGTTCCACAGAATGGTTTTCGCATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACTGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGTAGCCGGTGCAGTTTCA                                                                                  <  SRR19973061.3482511/150‑1 (MQ=60)
                                                                   CNAACACGCCAACCGGACCGTTCCACAGAATGGTTTTCGCATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACTGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGTAGCCGGTGCAGTTTCAG                                                                                 >  SRR19973061.1642097/1‑150 (MQ=60)
                                                                   CGAACACGCCAACCGGACCGTTCCACAGAATGGTTTTCGCATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACTGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGTAGCCGGTGCAGTTTCAG                                                                                 >  SRR19973061.3543650/1‑150 (MQ=60)
                                                                     AACACGCCAACCGGACCGTTCCACAGAATGGTTTTCGCATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACTGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGTAGCCGGTGCAGTTTCAGAG                                                                               <  SRR19973061.3340934/150‑1 (MQ=60)
                                                                     AACACGCCAACCGGACCGTTCCACAGAATGGTTTTCGCATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACTGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGTAGCCGGTGCAGTTTCAGAG                                                                               >  SRR19973061.1934532/1‑150 (MQ=60)
                                                                      ACACGCCAACCGGACCGTTCCACAGAATGGTTTTCGCATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACTGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGTAGCCGGTGCAGTTTCAGAGA                                                                              >  SRR19973061.3506634/1‑150 (MQ=60)
                                                                      ACACGCCAACCGGACCGTTCCACAGAATGGTTTTCGCATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACTGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGTAGCCGGTGCAGTTTCAGAGA                                                                              <  SRR19973061.1380086/150‑1 (MQ=60)
                                                                      ACACGCCAACCGGACCGTTCCACAGAATGGTTTTCGCATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACTGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGTAGCCGGTGCAGTTTCAGAGA                                                                              >  SRR19973061.888525/1‑150 (MQ=60)
                                                                          GCCAACCGGACCGTTCCACAGAATGGTTTTCGCATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACTGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGTAGCCGGTGCAGTTTCAGAGAACTC                                                                          >  SRR19973061.3459887/1‑150 (MQ=60)
                                                                          GCCAACCGGACCGTTCCACAGAATGGTTTTCGCATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACTGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGTAGCCGGTGCAGTTTCAGAGAACTC                                                                          <  SRR19973061.3728395/150‑1 (MQ=60)
                                                                           CCAACCGGACCGTTCCACAGAATGGTTTTCGCATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACTGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTCATGGTAGCCGGTGCAGTTTCAGAGAACTCG                                                                         >  SRR19973061.2903702/1‑150 (MQ=60)
                                                                             AACCGGACCGTTCCACAGAATGGTTTTCGCATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACTGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGTAGCCGGTGCAGTTTCAGAGAACTCGGT                                                                       >  SRR19973061.37611/1‑150 (MQ=60)
                                                                             AACCGGACCGTTCCACAGAATGGTTTTCGCATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACTGATATCCAGGATCTGCTAGTCAGCTTTCACATCGTTAACAGATTTCAGGGTAGCCGGTGCAGTTTCAGAGAACTCGGT                                                                       <  SRR19973061.961058/150‑1 (MQ=60)
                                                                               CCGGACCGTTCCACAGAATGGTTTTCGCATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACTGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGTAGCCGGTGCAGTTTCAGAGAACTCGGTTG                                                                     >  SRR19973061.3230295/1‑150 (MQ=60)
                                                                                 GGACCGTTCCACAGAATGGTTTTCGCATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACTGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGTAGCCGGTGCAGTTTCAGAGAACTCGGTTGCT                                                                   <  SRR19973061.912047/150‑1 (MQ=60)
                                                                                 GGACCGTTCCACAGAATGGTTTTCGCATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACTGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGTAGCCGGTGCAGTTTCAGAGAACTCGGTTGCT                                                                   >  SRR19973061.2731702/1‑150 (MQ=60)
                                                                                 GGACCGTTCCACAGAATGGTTTTCGCATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACTGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGTAGCCGGTGCAGTTTCAGAGAACTCGGTTGCT                                                                   >  SRR19973061.2527783/1‑150 (MQ=60)
                                                                                  GACCGTTCCACAGAATGGTTTTCGCATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACTGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGTAGCCGGTGCAGTTTCAGAGAACTCGGTTGCTA                                                                  >  SRR19973061.219278/1‑150 (MQ=60)
                                                                                   ACCGTTCCACAGAATGGTTTTCGCATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACTGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGTAGCCGGTGCAGTTTCAGAGAACTCGGTTGCTAC                                                                 >  SRR19973061.887848/1‑150 (MQ=60)
                                                                                    CCGTTCCACAGAATGGTTTTCGCATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACTGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGTAGCCGGTGCAGTTTCAGAGAACTCGGTTGCTACG                                                                >  SRR19973061.590593/1‑150 (MQ=60)
                                                                                     CGTTCCACAGAATGGTTTTCGCATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACTGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGTAGCCGGTGCAGTTTCAGAGAACTCGGTTGCTACGC                                                               >  SRR19973061.3701416/1‑150 (MQ=60)
                                                                                     CGTTCCACAGAATGGTTTTCGCATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACTGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGTAGCCGGTGCAGTTTCAGAGAACTCGGTTGCTACGC                                                               >  SRR19973061.3701714/1‑150 (MQ=60)
                                                                                             AGAATGGTTTTCGCATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACTGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGTAGCCGGTGCAGTTTCAGAGAACTCGGTTGCTACGCGAACATCA                                                       >  SRR19973061.2237482/1‑150 (MQ=60)
                                                                                                ATGGTTTTCGCATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACTGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGTAGCCGGTGCAGTTTCAGAGAACTCGGTTGCTACGCGAACATCAGAC                                                    <  SRR19973061.1352820/150‑1 (MQ=60)
                                                                                                ATGGTTTTCGCATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACTGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGTAGCCGGTGCAGTTTCAGAGAACTCGGTTGCTACGCGAACATCAGAC                                                    <  SRR19973061.1351687/150‑1 (MQ=60)
                                                                                                ATGGTTTTCGCATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACTGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGTAGCCGGTGCAGTTTCAGAGAACTCGGTTGCTACGCGAACATCAGAC                                                    <  SRR19973061.673442/150‑1 (MQ=60)
                                                                                                ATGGTTTTCGCATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACTGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGTAGCCGGTGCAGTTTCAGAGAACTCGGTTGCTACGCGAACATCAGAC                                                    <  SRR19973061.1135475/150‑1 (MQ=60)
                                                                                                ATGGTTTTCGCATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACTGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGTAGCCGGTGCAGTTTCAGAGAACTCGGTTGCTACGCGAACATCAGAC                                                    <  SRR19973061.1135363/150‑1 (MQ=60)
                                                                                                  GGTTTTCGCATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACTGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGTAGCCGGTGCAGTTTCAGAGAACTCGGTTGCTACGCGAACATCAGACGG                                                  >  SRR19973061.2006979/1‑150 (MQ=60)
                                                                                                  GGTTTTCGCATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACTGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGTAGCCGGTGCAGTTTCAGAGAACTCGGTTGCTACGCGAACATCAGACGG                                                  >  SRR19973061.2006730/1‑150 (MQ=60)
                                                                                                    TTTTCGCATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACTGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGTAGCCGGTGCAGTTTCAGAGAACTCGGTTGCTACGCGAACATCAGACGGAA                                                >  SRR19973061.2140092/1‑150 (MQ=60)
                                                                                                    TTTTCGCATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACTGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGTAGCCGGTGCAGTTTCAGAGAACTCGGTTGCTACGCGAACATCAGACGGAA                                                >  SRR19973061.2106240/1‑150 (MQ=60)
                                                                                                      TTCGCATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACTGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGTAGCCGGTGCAGTTTCAGAGAACTCGGTTGATACGCGAACATCAGACGGAACC                                              <  SRR19973061.2856985/150‑1 (MQ=60)
                                                                                                      TTCGCATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACTGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGTAGCCGGTGCAGTTTCAGAGAACTCGGTTGATACGCGAACATCAGACGGAACC                                              <  SRR19973061.2856579/150‑1 (MQ=60)
                                                                                                        CGCATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACTGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGTAGCCGGTGCAGTTTCAGAGAACTCGGTTGCTACGCGAACATCAGACGGAACCGG                                            <  SRR19973061.1451519/150‑1 (MQ=60)
                                                                                                         GCATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACTGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGTAGCCGGTGCAGTTTCAGAGAACTCGGTTGCTACGCGAACATCAGACGGAACCGGG                                           >  SRR19973061.2365939/1‑150 (MQ=60)
                                                                                                          CATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACTGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGTAGCCGGTGCAGTTTCAGAGAACTCGGTTGCTACGCGAACATCAGACGGAACCGGGA                                          >  SRR19973061.87642/1‑150 (MQ=60)
                                                                                                           ATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACTGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGTAGCCGGTGCAGTTTCAGAGAACTCGGTTGCTACGCGAACATCAGACGGAACCGGGAT                                         >  SRR19973061.3437660/1‑150 (MQ=60)
                                                                                                            TTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACTGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGTAGCCGGTGCAGTTTCAGAGAACTCGGTTGCTACGCGAACATCAGACGGAACCGGGATG                                        >  SRR19973061.846815/1‑150 (MQ=60)
                                                                                                              CTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACTGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGTAGCCGGTGCAGTTTCAGAGAACTCGGTTGCTACGCGAACATCAGACGGAACCGGGATGTT                                      <  SRR19973061.1323819/150‑1 (MQ=60)
                                                                                                              CTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACTGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGTAGCCGGTGCAGTTTCAGAGAACTCGGTTGCTACGCGAACATCAGACGGAACCGGGATGTT                                      <  SRR19973061.3598435/150‑1 (MQ=60)
                                                                                                                TCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACTGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGTAGCCGGTGCAGTTTCAGAGAACTCGGTTGCTACGCGAACATCAGACGGAACCGGGATGTTGC                                    <  SRR19973061.3019450/150‑1 (MQ=60)
                                                                                                                  AGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACTGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGTAGCCGGTGCAGTTTCAGAGAACTCGGTTGCTACGCGAACATCAGACGGAACCGGGATGTTGCAG                                  <  SRR19973061.6188/150‑1 (MQ=60)
                                                                                                                   GGATTTCAGCCAGTTCCTGAGCGGAAGCATCACTGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGTAGCCGGTGCAGTTTCAGAGAACTCGGTTGCTACGCGAACATCAGACGGAACCGGGATGTTGCAGG                                 <  SRR19973061.3650940/150‑1 (MQ=60)
                                                                                                                   GGATTTCAGCCAGTTCCTGAGCGGAAGCATCACTGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGTAGCCGGTGCAGTTTCAGAGAACTCGGTTGCTACGCGAACATCAGACGGAACCGGGATGTTGCAGG                                 <  SRR19973061.3723772/150‑1 (MQ=60)
                                                                                                                      TTTCAGCCAGTTCCTGAGCGGAAGCATCACTGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGTAGCCGGTGCAGTTTCAGAGAACTCGGTTGCTACGCGAACATCAGACGGAACCGGGATGTTGCAGGTGG                              >  SRR19973061.114328/1‑150 (MQ=60)
                                                                                                                         CAGCCAGTTCCTGAGCGGAAGCATCACTGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGTAGCCGGTGCAGTTTCAGAGAACTCGGTTGCTACGCGAACATCAGACGGAACCGGGATGTTGCAGGTGGTCA                           <  SRR19973061.3698382/150‑1 (MQ=60)
                                                                                                                          AGCCAGTTCCTGAGCGGAAGCATCACTGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGTAGCCGGTGCAGTTTCAGAGAACTCGGTTGCTACGCGAACATCAGACGGAACCGGGATGTTGCAGGTGGTCAG                          >  SRR19973061.2754009/1‑150 (MQ=60)
                                                                                                                            CCAGTTCCTGAGCGGAAGCATCACTGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGTAGCCGGTGCAGTTTCAGAGAACTCGGTTGCTACGCGAACATCAGACGGAACCGGGATGTTGCAGGTGGTCAGCA                        <  SRR19973061.3433658/150‑1 (MQ=60)
                                                                                                                            CCAGTTCCTGAGCGGAAGCATCACTGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGTAGCCGGTGCAGTTTCAGAGAACTCGGTTGCTACGCGAACATCAGACGGAACCGGGATGTTGCAGGTGGTCAGCA                        <  SRR19973061.1482375/150‑1 (MQ=60)
                                                                                                                             CAGTTCCTGAGCGGAAGCATCACTGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGTAGCCGGTGCAGTTTCAGAGAACTCGGTTGCTACGCGAACATCAGACGGAACCGGGATGTTGCAGGTGGTCAGCAG                       <  SRR19973061.3761464/150‑1 (MQ=60)
                                                                                                                              AGTTCCTGAGCGGAAGCATCACTGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGTAGCCGGTGCAGTTTCAGAGAACTCGGTTGCTACGCGAACATCAGACGGAACCGGGATGTTGCAGGTGGTCAGCAGA                      >  SRR19973061.1401286/1‑150 (MQ=60)
                                                                                                                              AGTTCCTGAGCGGAAGCATCACTGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGTAGCCGGTGCAGTTTCAGAGAACTCGGTTGCTACGCGAACATCAGACGGAACCGGGATGTTGCAGGTGGTCAGCAGA                      >  SRR19973061.2736355/1‑150 (MQ=60)
                                                                                                                                   CTGAGCGGAAGCATCACTGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGTAGCCGGTGCAGTTTCAGAGAACTCGGTTGCTACGCGAACATCAGACGGAACCGGGATGTTGCAGGTGGTCAGCAGACGTTT                 <  SRR19973061.820650/150‑1 (MQ=60)
                                                                                                                                    TGAGCGGAAGCATCACTGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGTAGCCGGTGCAGTTTCAGAGAACTCGGTTGCTACGCGAACATCAGACGGAACCGGGATGTTGCAGGTGGTCAGCAGACGTTTA                >  SRR19973061.3740051/1‑150 (MQ=60)
                                                                                                                                         GGAAGCATCACTGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGTAGCCGGTGCAGTTTCAGAGAACTCGGTTGCTACGCGAACATCAGACGGAACCGGGATGTTGCAGGTGGTCAGCAGACGTTTAGCTTC           <  SRR19973061.578405/150‑1 (MQ=60)
                                                                                                                                          GAAGCATCACTGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGTAGCCGGTGCAGTTTCAGAGAACTCGGTTGCTACGCGAACATCAGACGGAACCGGGATGTTGCAGGTGGTCAGCAGACGTTTAGCTTCG          >  SRR19973061.531029/1‑150 (MQ=60)
                                                                                                                                           AAGCATCACTGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGTAGCCGGTGCAGTTTCAGAGAACTCGGTTGCTACGCGAACATCAGACGGAACCGGGATGTTGCAGGTGGTCAGCAGACGTTTAGCTTCGT         >  SRR19973061.360016/1‑150 (MQ=60)
                                                                                                                                           AAGCATCACTGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGTAGCCGGTGCAGTTTCAGAGAACTCGGTTGCTACGCGAACATCAGACGGAACCGGGATGTTGCAGGTGGTCAGCAGACGTTTAGCTTCGT         >  SRR19973061.360018/1‑150 (MQ=60)
                                                                                                                                                 CACTGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGTAGCCGGTGCAGTTTCAGAGAACTCGGTTGCTACGCGAACATCAGACGGAACCGGGATGTTGCAGGTGGTCAGCAGACGTTTAGCTTCGTCAACCA   <  SRR19973061.3363119/150‑1 (MQ=60)
                                                                                                                                                 CACTGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGTAGCCGGTGCAGTTTCAGAGAACTCGGTTGCTACGCGAACATCAGACGGAACCGGGATGTTGCAGGTGGTCAGCAGACGTTTAGCTTCGTCAACCA   >  SRR19973061.411925/1‑150 (MQ=60)
                                                                                                                                                  ACTGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGTAGCCGGTGCAGTTTCAGAGAACTCGGTTGCTACGCGAACATCAGACGGAACCGGGATGTTGCAGGTGGTCAGCAGACGTTTAGCTTCGTCAACCAG  <  SRR19973061.308273/150‑1 (MQ=60)
                                                                                                                                                  ACTGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGTAGCCGGTGCAGTTTCAGAGAACTCGGTTGCTACGCGAACATCAGACGGAACCGGGATGTTGCAGGTGGTCAGCAGACGTTTAGCTTCGTCAACCAG  >  SRR19973061.3766265/1‑150 (MQ=60)
                                                                                                                                                  ACTGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGTAGCCGGTGCAGTTTCAGAGAACTCGGTTGCTACGCGAACATCAGACGGAACCGGGATGTTGCAGGTGGTCAGCAGACGTTTAGCTTCGTCAACCAG  <  SRR19973061.963111/150‑1 (MQ=60)
                                                                                                                                                    |                                                                                                                                                   
GAGAACGCTTCGCTGTCTGCGATAGCGTTAGCCACGATTTCAGTACCTTTGCGGAAGTTCGGGAATTCGAACACGCCAACCGGACCGTTCCACAGAATGGTTTTCGCATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACCGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGTAGCCGGTGCAGTTTCAGAGAACTCGGTTGCTACGCGAACATCAGACGGAACCGGGATGTTGCAGGTGGTCAGCAGACGTTTAGCTTCGTCAACCAG  >  CP009273/3064975‑3065270

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑