Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F534 I1 R1 1235 230.2 7611948 95.1% 7238962 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009273 3,110,213 T→A A753A (GCA→GCT)  yghJ ← DUF4092 family putative lipoprotein peptidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,110,2130TA100.0% 679.6 / NA 202A753A (GCA→GCT) yghJDUF4092 family putative lipoprotein peptidase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (91/111);  total (91/111)

Only 100 of 202 total aligned reads displayed.

AACTTCCCCTGTCTTACTATCGATGGTGTACGGCAGCGCACCATCTACGGCAGGATAACGTTCATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGTGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCACCGATCCACCTTTGTTCAGATAGGCGATCAGATCGGTCACATCCTGCTGAGTCA  >  CP009273/3110066‑3110358
                                                                                                                                                   |                                                                                                                                                 
aaCTTCCCCTGTCTTACTATCGATGGTGTACGGCAGCGCACCATCTACGGCAGGATAACGTTCATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGc                                                                                                                                                 >  1:647515/1‑150 (MQ=255)
  cTTCCCCTGTCTTACTATCGATGGTGTACGGCAGCGCACCATCTACGGCAGGATAACGTTCATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCa                                                                                                                                               >  1:1211761/1‑150 (MQ=255)
    tCCCCTGTCTTACTATCGATGGTGTACGGCAGCGCACCATCTACGGCAGGATAACGTTCATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATc                                                                                                                                             <  1:1239911/150‑1 (MQ=255)
    tCCCCTGTCTTACTATCGATGGTGTACGGCAGCGCACCATCTACGGCAGGATAACGTTCATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATc                                                                                                                                             <  1:1745429/150‑1 (MQ=255)
     ccccTGTCTTACTATCGATGGTGTACGGCAGCGCACCATCTACGGCAGGATAACGTTCATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCg                                                                                                                                            >  2:3428378/1‑150 (MQ=255)
     ccccTGTCTTACTATCGATGGTGTACGGCAGCGCACCATCTACGGCAGGATAACGTTCATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCg                                                                                                                                            <  1:26706/150‑1 (MQ=255)
     ccccTGTCTTACTATCGATGGTGTACGGCAGCGCACCATCTACGGCAGGATAACGTTCATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCg                                                                                                                                            <  1:367340/150‑1 (MQ=255)
     ccccTGTCTTACTATCGATGGTGTACGGCAGCGCACCATCTACGGCAGGATAACGTTCATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCg                                                                                                                                            >  2:1819819/1‑150 (MQ=255)
       ccTGTCTTACTATCGATGGTGTACGGCAGCGCACCATCTACGGCAGGATAACGTTCATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGAc                                                                                                                                          <  1:666716/150‑1 (MQ=255)
       ccTGTCTTACTATCGATGGTGTACGGCAGCGCACCATCTACGGCAGGATAACGTTCATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGAc                                                                                                                                          >  2:1741477/1‑150 (MQ=255)
       ccTGTCTTACTATCGATGGTGTACGGCAGCGCACCATCTACGGCAGGATAACGTTCATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGAc                                                                                                                                          >  2:2651134/1‑150 (MQ=255)
         tGTCTTACTATCGATGGTGTACGGCAGCGCACCATCTACGGCAGGATAACGTTCATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAg                                                                                                                                        <  1:2823537/150‑1 (MQ=255)
             ttACTATCGATGGTGTACGGCAGCGCACCATCTACGGCAGGATAACGTTCATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCt                                                                                                                                    >  2:2044531/1‑150 (MQ=255)
               aCTATCGATGGTGTACGGCAGCGCACCATCTACGGCAGGATAACGTTCATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTACGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGc                                                                                                                                  >  2:170959/1‑150 (MQ=255)
                cTATCGATGGTGTACGGCAGCGCACCATCTACGGCAGGATAACGTTCATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCg                                                                                                                                 <  2:900374/150‑1 (MQ=255)
                      aTGGTGTACGGCAGCGCACCATCTACGGCAGGATAACGTTCATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATcc                                                                                                                           >  2:491680/1‑150 (MQ=255)
                       tGGTGTACGGCAGCGCACCATCTACGGCAGGATAACGTTCATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCa                                                                                                                          <  2:3011071/150‑1 (MQ=255)
                       tGGTGTACGGCAGCGCACCATCTACGGCAGGATAACGTTCATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCa                                                                                                                          <  2:3357628/150‑1 (MQ=255)
                          tgtACGGCAGCGCACCATCTACGGCAGGATAACGTTCATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACa                                                                                                                       >  2:1965300/1‑150 (MQ=255)
                             aCGGCAGCGCACCATCTACGGCAGGATAACGTTCATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGAc                                                                                                                    <  1:1542835/150‑1 (MQ=255)
                             aCGGCAGCGCACCATCTACGGCAGGATAACGTTCATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGAc                                                                                                                    >  2:3399803/1‑150 (MQ=255)
                               ggCAGCGCACCATCTACGGCAGGATAACGTTCATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGc                                                                                                                  >  1:2886287/1‑150 (MQ=255)
                                   gcgcACCATCTACGGCAGGATAACGTTCATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACaa                                                                                                              >  2:601161/1‑150 (MQ=255)
                                      cACCATCTACGGCAGGATAACGTTCATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAAc                                                                                                           >  2:1424349/1‑150 (MQ=255)
                                      cACCATCTACGGCAGGATAACGTTCATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAAc                                                                                                           >  1:1063449/1‑150 (MQ=255)
                                       aCCATCTACGGCAGGATAACGTTCATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAAcc                                                                                                          >  1:484658/1‑150 (MQ=255)
                                           tCTACGGCAGGATAACGTTCATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGAc                                                                                                      <  2:2186894/150‑1 (MQ=255)
                                            cTACGGCAGGATAACGTTCATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGAcg                                                                                                     >  1:1424484/1‑150 (MQ=255)
                                             tACGGCAGGATAACGTTCATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGAcgc                                                                                                    >  1:3034200/1‑150 (MQ=255)
                                                     gATAACGTTCATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCtt                                                                                            >  2:1310108/1‑150 (MQ=255)
                                                     gATAACGTTCATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCtt                                                                                            <  2:2459665/150‑1 (MQ=255)
                                                      aTAACGTTCATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTc                                                                                           <  1:2604543/150‑1 (MQ=255)
                                                       tAACGTTCATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTcc                                                                                          >  1:1952616/1‑150 (MQ=255)
                                                         aCGTTCATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCtt                                                                                        >  1:1383401/1‑150 (MQ=255)
                                                           gTTCATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTaa                                                                                      <  1:2708793/150‑1 (MQ=255)
                                                           gTTCATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTaa                                                                                      >  1:2518760/1‑150 (MQ=255)
                                                           gTTCATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCATTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTac                                                                                      <  1:1578072/150‑2 (MQ=255)
                                                            ttCATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAg                                                                                     <  1:1648709/150‑1 (MQ=255)
                                                              cATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGAt                                                                                   <  2:1867431/150‑1 (MQ=255)
                                                              cATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGAt                                                                                   <  1:1860789/150‑1 (MQ=255)
                                                              cATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGAt                                                                                   >  2:3070740/1‑150 (MQ=255)
                                                               aTAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGAtt                                                                                  <  1:3294914/150‑1 (MQ=255)
                                                                  gACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCt                                                                               <  1:1121029/150‑1 (MQ=255)
                                                                       aaaTGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCtcatca                                                                          >  2:1675131/1‑150 (MQ=255)
                                                                       aaaTGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCtcatca                                                                          >  2:927062/1‑150 (MQ=255)
                                                                        aaTGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATAAc                                                                         >  2:3574796/1‑150 (MQ=255)
                                                                         aTGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACg                                                                        >  1:1794252/1‑150 (MQ=255)
                                                                             ccGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGtttt                                                                    <  2:3098223/150‑1 (MQ=255)
                                                                             ccGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGtttt                                                                    >  1:558577/1‑150 (MQ=255)
                                                                               gTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTcc                                                                  <  1:310754/150‑1 (MQ=255)
                                                                                ttGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCa                                                                 <  2:1521248/150‑1 (MQ=255)
                                                                                 tGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCAt                                                                <  2:1239279/150‑1 (MQ=255)
                                                                                    gcgcTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGAt                                                             <  2:1095431/150‑1 (MQ=255)
                                                                                      gctgctGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCa                                                           >  1:3438308/1‑150 (MQ=255)
                                                                                      gctgctGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCa                                                           <  2:1440/150‑1 (MQ=255)
                                                                                      gctgctGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCa                                                           <  2:784733/150‑1 (MQ=255)
                                                                                       ctgctgACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAg                                                          <  1:824267/150‑1 (MQ=255)
                                                                                       ctgctgACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAg                                                          <  1:2969317/150‑1 (MQ=255)
                                                                                         gctgACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCa                                                        <  1:3754312/150‑1 (MQ=255)
                                                                                          ctgACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCAc                                                       <  2:1031905/150‑1 (MQ=255)
                                                                                          ctgACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCAc                                                       >  2:2971572/1‑150 (MQ=255)
                                                                                          ctgACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCACTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCAc                                                       >  1:511933/1‑150 (MQ=255)
                                                                                           tgACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCAcc                                                      <  1:2763515/150‑1 (MQ=255)
                                                                                           tgACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCAcc                                                      <  1:2335543/150‑1 (MQ=255)
                                                                                            gACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCACCg                                                     <  2:2673980/150‑1 (MQ=255)
                                                                                                aaCGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCACCGATcc                                                 >  2:453527/1‑150 (MQ=255)
                                                                                                   gcgGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCACCGATCCAcc                                              <  2:248807/150‑1 (MQ=255)
                                                                                                   gcgGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCACCGATCCAcc                                              >  1:2298441/1‑150 (MQ=255)
                                                                                                    cgGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCACCGATCCACCt                                             >  2:94815/1‑150 (MQ=255)
                                                                                                     ggTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCACCGATCCACCtt                                            >  2:718684/1‑150 (MQ=255)
                                                                                                     ggTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCACCGATCCACCtt                                            >  1:403431/1‑150 (MQ=255)
                                                                                                      gTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCACCGATCCACCttt                                           <  1:3510220/150‑1 (MQ=255)
                                                                                                        tCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCACCGATCCACCTTTGt                                         <  2:2732625/150‑1 (MQ=255)
                                                                                                          ggATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCACCGATCCACCTTTGTTc                                       <  1:3399803/150‑1 (MQ=255)
                                                                                                           gATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCACCGATCCACCTTTGTTCa                                      <  1:539640/150‑1 (MQ=255)
                                                                                                            aTACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCACCGATCCACCTTTGTTCAg                                     >  1:934379/1‑150 (MQ=255)
                                                                                                             tACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCACCGATCCACCTTTGTTCAGa                                    <  2:1534235/150‑1 (MQ=255)
                                                                                                              aCCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCACCGATCCACCTTTGTTCAGAt                                   <  1:1756639/150‑1 (MQ=255)
                                                                                                                    gCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCACCGATCCACCTTTGTTCAGATAGGCGa                             <  1:1179100/150‑1 (MQ=255)
                                                                                                                    gCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCACCGATCCACCTTTGTTCAGATAGGCGa                             <  2:3190352/150‑1 (MQ=255)
                                                                                                                      ggATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCACCGATCCACCTTTGTTCAGATAGGCGATc                           >  1:3614161/1‑150 (MQ=255)
                                                                                                                      ggATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCACCGATCCACCTTTGTTCAGATAGGCGATc                           <  1:1741477/150‑1 (MQ=255)
                                                                                                                      ggATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCACCGATCCACCTTTGTTCAGATAGGCGATc                           >  2:389461/1‑150 (MQ=255)
                                                                                                                       gATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCACCGATCCACCTTTGTTCAGATAGGCGATCa                          <  2:2490694/150‑1 (MQ=255)
                                                                                                                          cGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCACCGATCCACCTTTGTTCAGATAGGCGATCAGAt                       <  2:2802850/150‑1 (MQ=255)
                                                                                                                            ttATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCACCGATCCACCTTTGTTCAGATAGGCGATCAGATCg                     >  2:2277584/1‑150 (MQ=255)
                                                                                                                              aTTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCACCGATCCACCTTTGTTCAGATAGGCGATCAGATCGGt                   <  2:2339627/150‑1 (MQ=255)
                                                                                                                                ttACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCACCGATCCACCTTTGTTCAGATAGGCGATCAGATCGGTca                 >  2:35680/1‑150 (MQ=255)
                                                                                                                                ttACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCACCGATCCACCTTTGTTCAGATAGGCGATCAGATCGGTca                 <  2:3579710/150‑1 (MQ=255)
                                                                                                                                    tacCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCACCGATCCACCTTTGTTCAGATAGGCGATCAGATCGGTCACATc             <  1:853949/150‑1 (MQ=255)
                                                                                                                                    tacCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCACCGATCCACCTTTGTTCAGATAGGCGATCAGATCGGTCACATc             >  1:149163/1‑150 (MQ=255)
                                                                                                                                     acCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCACCGATCCACCTTTGTTCAGATAGGCGATCAGATCGGTCACATcc            >  2:2580577/1‑150 (MQ=255)
                                                                                                                                      ccGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCACCGATCCACCTTTGTTCAGATAGGCGATCAGATCGGTCACATCct           <  2:3438405/150‑1 (MQ=255)
                                                                                                                                         aCTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCACCGATCCACCTTTGTTCAGATAGGCGATCAGATCGGTCACATCctgct        <  1:762657/150‑1 (MQ=255)
                                                                                                                                          cTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCACCGATCCACCTTTGTTCAGATAGGCGATCAGATCGGTCACATCctgctg       >  2:3722300/1‑150 (MQ=255)
                                                                                                                                          cTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCACCGATCCACCTTTGTTCAGATAGGCGATCAGATCGGTCACATCctgctg       >  1:1979976/1‑150 (MQ=255)
                                                                                                                                            tGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCACCGATCCACCTTTGTTCAGATAGGCGATCAGATCGGTCACATCCTGCTGAg     >  1:2086648/1‑150 (MQ=255)
                                                                                                                                              ttCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCACCGATCCACCTTTGTTCAGATAGGCGATCAGATCGGTCACATCCTGCTGAGTc   <  1:248264/150‑1 (MQ=255)
                                                                                                                                               tCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCACCGATCCACCTTTGTTCAGATAGGCGATCAGATCGGTCACATCCTGCTGAGTCa  <  2:1610888/150‑1 (MQ=255)
                                                                                                                                               tCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCACCGATCCACCTTTGTTCAGATAGGCGATCAGATCGGTCACATCCTGCTGAGTCa  >  1:3391272/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                 
AACTTCCCCTGTCTTACTATCGATGGTGTACGGCAGCGCACCATCTACGGCAGGATAACGTTCATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGTGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCACCGATCCACCTTTGTTCAGATAGGCGATCAGATCGGTCACATCCTGCTGAGTCA  >  CP009273/3110066‑3110358

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TTAACTTCCCCTGTCTTACTATCGATGGTGTACGGCAGCGCACCATCTACGGCAGGATAACGTTCATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGTGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCACCGATCCACCTTTGTTCAGATAGGCGATCAGATCGGTCACATCCTGCTGAGTCAGCTT  >  CP009273/3110064‑3110362
                                                                                                                                                     |                                                                                                                                                     
TTAACTTCCCCTGTCTTACTATCGATGGTGTACGGCAGCGCACCATCTACGGCAGGATAACGTTCATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGA                                                                                                                                                       <  SRR19973061.3272965/150‑1 (MQ=60)
 TAACTTCCCCTGTCTTACTATCGATGGTGTACGGCAGCGCACCATCTACGGCAGGATAACGTTCATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAG                                                                                                                                                      <  SRR19973061.3530758/150‑1 (MQ=60)
  AACTTCCCCTGTCTTACTATCGATGGTGTACGGCAGCGCACCATCTACGGCAGGATAACGTTCATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGC                                                                                                                                                     >  SRR19973061.649177/1‑150 (MQ=60)
    CTTCCCCTGTCTTACTATCGATGGTGTACGGCAGCGCACCATCTACGGCAGGATAACGTTCATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCA                                                                                                                                                   >  SRR19973061.1214798/1‑150 (MQ=60)
     TTCCCCTGTCTTACTATCGATGGTGTACGGCAGCGCACCATCTACGGCAGGATAACGTTCATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCAT                                                                                                                                                  <  SRR19973061.1719813/150‑1 (MQ=60)
      TCCCCTGTCTTACTATCGATGGTGTACGGCAGCGCACCATCTACGGCAGGATAACGTTCATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATC                                                                                                                                                 <  SRR19973061.1749814/150‑1 (MQ=60)
      TCCCCTGTCTTACTATCGATGGTGTACGGCAGCGCACCATCTACGGCAGGATAACGTTCATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATC                                                                                                                                                 <  SRR19973061.1242990/150‑1 (MQ=60)
       CCCCTGTCTTACTATCGATGGTGTACGGCAGCGCACCATCTACGGCAGGATAACGTTCATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCG                                                                                                                                                <  SRR19973061.368337/150‑1 (MQ=60)
       CCCCTGTCTTACTATCGATGGTGTACGGCAGCGCACCATCTACGGCAGGATAACGTTCATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCG                                                                                                                                                <  SRR19973061.26806/150‑1 (MQ=60)
       CCCCTGTCTTACTATCGATGGTGTACGGCAGCGCACCATCTACGGCAGGATAACGTTCATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCG                                                                                                                                                <  SRR19973061.27348/150‑1 (MQ=60)
         CCTGTCTTACTATCGATGGTGTACGGCAGCGCACCATCTACGGCAGGATAACGTTCATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGAC                                                                                                                                              <  SRR19973061.3375417/150‑1 (MQ=60)
         CCTGTCTTACTATCGATGGTGTACGGCAGCGCACCATCTACGGCAGGATAACGTTCATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGAC                                                                                                                                              <  SRR19973061.668439/150‑1 (MQ=60)
           TGTCTTACTATCGATGGTGTACGGCAGCGCACCATCTACGGCAGGATAACGTTCATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAG                                                                                                                                            <  SRR19973061.2830772/150‑1 (MQ=60)
                 ACTATCGATGGTGTACGGCAGCGCACCATCTACGGCAGGATAACGTTCATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGC                                                                                                                                      <  SRR19973061.1937143/150‑1 (MQ=60)
                         TGGTGTACGGCAGCGCACCATCTACGGCAGGATATCGTTCATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCA                                                                                                                              <  SRR19973061.1754562/150‑1 (MQ=60)
                         TGGTGTACGGCAGCGCACCATCTACGGCAGGATAACGTTCATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCA                                                                                                                              >  SRR19973061.2612390/1‑150 (MQ=60)
                               ACGGCAGCGCACCATCTACGGCAGGATAACGTTCATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGAC                                                                                                                        <  SRR19973061.1546673/150‑1 (MQ=60)
                                CGGCAGCGCACCATCTACGGCAGGATAACGTTCATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACG                                                                                                                       >  SRR19973061.2810049/1‑150 (MQ=60)
                                 GGCAGCGCACCATCTACGGCAGGATAACGTTCATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGC                                                                                                                      >  SRR19973061.2893686/1‑150 (MQ=60)
                                       GCACCATCTACGGCAGGATAACGTTCATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAA                                                                                                                >  SRR19973061.3115095/1‑150 (MQ=60)
                                        CACCATCTACGGCAGGATAACGTTCATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAAC                                                                                                               >  SRR19973061.1066180/1‑150 (MQ=60)
                                         ACCATCTACGGCAGGATAACGTTCATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACC                                                                                                              >  SRR19973061.485954/1‑150 (MQ=60)
                                         ACCATCTACGGCAGGATAACGTTCATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACC                                                                                                              >  SRR19973061.486293/1‑150 (MQ=60)
                                          CCATCTACGGCAGGATAACGTTCATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCA                                                                                                             >  SRR19973061.2227027/1‑150 (MQ=60)
                                          CCATCTACGGCAGGATAACGTTCATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCA                                                                                                             <  SRR19973061.62955/150‑1 (MQ=60)
                                           CATCTACGGCAGGATAACGTTCATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAG                                                                                                            <  SRR19973061.1982255/150‑1 (MQ=60)
                                              CTACGGCAGGATAACGTTCATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACG                                                                                                         >  SRR19973061.1428072/1‑150 (MQ=60)
                                              CTACGGCAGGATAACGTTCATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACG                                                                                                         <  SRR19973061.3160373/150‑1 (MQ=60)
                                               TACGGCAGGATAACGTTCATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGC                                                                                                        >  SRR19973061.3042040/1‑150 (MQ=60)
                                                 CGGCAGGATAACGTTCATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGC                                                                                                      <  SRR19973061.1747332/150‑1 (MQ=60)
                                                    CAGGATAACGTTCATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCT                                                                                                   <  SRR19973061.3752575/150‑1 (MQ=60)
                                                      GGATAACGTTCATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCT                                                                                                 <  SRR19973061.1641222/150‑1 (MQ=60)
                                                       GATAACGTTCATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTT                                                                                                >  SRR19973061.1450304/1‑150 (MQ=60)
                                                        ATAACGTTCATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTC                                                                                               <  SRR19973061.2611236/150‑1 (MQ=60)
                                                         TAACGTTCATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCC                                                                                              >  SRR19973061.1957492/1‑150 (MQ=60)
                                                         TAACGTTCATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCC                                                                                              >  SRR19973061.2679076/1‑150 (MQ=60)
                                                           ACGTTCATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTT                                                                                            >  SRR19973061.1386904/1‑150 (MQ=60)
                                                             GTTCATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAA                                                                                          <  SRR19973061.807013/150‑1 (MQ=60)
                                                             GTTCATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAA                                                                                          <  SRR19973061.2715766/150‑1 (MQ=60)
                                                             GTTCATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAA                                                                                          >  SRR19973061.2525179/1‑150 (MQ=60)
                                                             GTTCATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAA                                                                                          >  SRR19973061.248038/1‑150 (MQ=60)
                                                             GTTCATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCATTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAC                                                                                          <  SRR19973061.1581993/150‑1 (MQ=60)
                                                              TTCATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAG                                                                                         <  SRR19973061.1652850/150‑1 (MQ=60)
                                                                CATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGAT                                                                                       <  SRR19973061.1865431/150‑1 (MQ=60)
                                                                 ATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATT                                                                                      <  SRR19973061.3303397/150‑1 (MQ=60)
                                                                  TAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTG                                                                                     <  SRR19973061.1233668/150‑1 (MQ=60)
                                                                    GACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCT                                                                                   <  SRR19973061.1123895/150‑1 (MQ=60)
                                                                    GACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCT                                                                                   <  SRR19973061.544370/150‑1 (MQ=60)
                                                                     ACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTC                                                                                  >  SRR19973061.2956806/1‑150 (MQ=60)
                                                                          AATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCAC                                                                             <  SRR19973061.1427937/150‑1 (MQ=60)
                                                                           ATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACG                                                                            >  SRR19973061.1798742/1‑150 (MQ=60)
                                                                             GCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTT                                                                          <  SRR19973061.109778/150‑1 (MQ=60)
                                                                             GCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTT                                                                          <  SRR19973061.1824377/150‑1 (MQ=60)
                                                                             GCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTT                                                                          <  SRR19973061.2024851/150‑1 (MQ=60)
                                                                               CCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTT                                                                        >  SRR19973061.560047/1‑150 (MQ=60)
                                                                                 GTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCC                                                                      <  SRR19973061.311570/150‑1 (MQ=60)
                                                                                        GCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCA                                                               >  SRR19973061.3447224/1‑150 (MQ=60)
                                                                                        GCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCA                                                               >  SRR19973061.3447125/1‑150 (MQ=60)
                                                                                         CTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAG                                                              <  SRR19973061.826414/150‑1 (MQ=60)
                                                                                         CTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAG                                                              <  SRR19973061.2976973/150‑1 (MQ=60)
                                                                                           GCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCA                                                            <  SRR19973061.3763920/150‑1 (MQ=60)
                                                                                            CTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCACTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCAC                                                           >  SRR19973061.513285/1‑150 (MQ=60)
                                                                                             TGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCACC                                                          <  SRR19973061.2770631/150‑1 (MQ=60)
                                                                                             TGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCACC                                                          <  SRR19973061.2341472/150‑1 (MQ=60)
                                                                                              GACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCACCG                                                         >  SRR19973061.834526/1‑150 (MQ=60)
                                                                                                     GCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCACCGATCCACC                                                  >  SRR19973061.2304257/1‑150 (MQ=60)
                                                                                                     GCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCACCGATCCACC                                                  >  SRR19973061.2304796/1‑150 (MQ=60)
                                                                                                       GGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCACCGATCCACCTT                                                >  SRR19973061.404527/1‑150 (MQ=60)
                                                                                                        GTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCACCGATCCACCTTT                                               <  SRR19973061.3519204/150‑1 (MQ=60)
                                                                                                            GGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCACCGATCCACCTTTGTTC                                           <  SRR19973061.3408533/150‑1 (MQ=60)
                                                                                                             GATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCTGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCACCGATCCACCTTTGTTCA                                          <  SRR19973061.128403/150‑1 (MQ=60)
                                                                                                             GATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCACCGATCCACCTTTGTTCA                                          <  SRR19973061.541061/150‑1 (MQ=60)
                                                                                                             GATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCACCGATCCACCTTTGTTCA                                          <  SRR19973061.539689/150‑1 (MQ=60)
                                                                                                              ATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCACCGATCCACCTTTGTTCAG                                         >  SRR19973061.936757/1‑150 (MQ=60)
                                                                                                                ACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCACCGATCCACCTTTGTTCAGAT                                       <  SRR19973061.1761041/150‑1 (MQ=60)
                                                                                                                 CCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCACCGATCCACCTTTGTTCAGATA                                      <  SRR19973061.3032324/150‑1 (MQ=60)
                                                                                                                 CCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCACCGATCCACCTTTGTTCAGATA                                      >  SRR19973061.1559135/1‑150 (MQ=60)
                                                                                                                  CCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCACCGATCCACCTTTGTTCAGATAG                                     >  SRR19973061.1200852/1‑150 (MQ=60)
                                                                                                                  CCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCACCGATCCACCTTTGTTCAGATAG                                     <  SRR19973061.3423731/150‑1 (MQ=60)
                                                                                                                      GCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCACCGATCCACCTTTGTTCAGATAGGCGA                                 <  SRR19973061.1182082/150‑1 (MQ=60)
                                                                                                                      GCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCACCGATCCACCTTTGTTCAGATAGGCGA                                 <  SRR19973061.980713/150‑1 (MQ=60)
                                                                                                                        GGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCACCGATCCACCTTTGTTCAGATAGGCGATC                               <  SRR19973061.2557342/150‑1 (MQ=60)
                                                                                                                        GGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCACCGATCCACCTTTGTTCAGATAGGCGATC                               <  SRR19973061.1745855/150‑1 (MQ=60)
                                                                                                                        GGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCACCGATCCACCTTTGTTCAGATAGGCGATC                               >  SRR19973061.3623409/1‑150 (MQ=60)
                                                                                                                        GGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCACCGATCCACCTTTGTTCAGATAGGCGATC                               >  SRR19973061.3566866/1‑150 (MQ=60)
                                                                                                                         GATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCACCGATCCACCTTTGTTCAGATAGGCGATCA                              <  SRR19973061.2500815/150‑1 (MQ=60)
                                                                                                                            CGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCACCGATCCACCTTTGTTCAGATAGGCGATCAGAT                           <  SRR19973061.1531316/150‑1 (MQ=60)
                                                                                                                             GTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCACCGATCCACCTTTGTTCAGATAGGCGCTCAGAGC                          >  SRR19973061.3401699/1‑150 (MQ=60)
                                                                                                                              TTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCACCGATCCACCTTTGTTCAGATAGGCGATCAGATCG                         <  SRR19973061.3799259/150‑1 (MQ=60)
                                                                                                                              TTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCACCGATCCACCTTTGTTCAGATAGGCGATCAGATCG                         <  SRR19973061.466950/150‑1 (MQ=60)
                                                                                                                              TTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCACCGATCCACCTTTGTTCAGATAGGCGATCAGATCG                         <  SRR19973061.903585/150‑1 (MQ=60)
                                                                                                                              TTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTATCCATGATCAGCACCGATCCACCTTTGTTCAGATATGCGATCAGATCG                         >  SRR19973061.1795575/1‑150 (MQ=60)
                                                                                                                                ATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCACCGATCCACCTTTGTTCAGATAGGCGATCAGATCGGT                       >  SRR19973061.2841343/1‑150 (MQ=60)
                                                                                                                                ATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCACCGATCCACCTTTGTTCAGATAGGCGATCAGATCGGT                       <  SRR19973061.907288/150‑1 (MQ=60)
                                                                                                                                      TACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCACCGATCCACCTTTGTTCAGATAGGCGATCAGATCGGTCACATC                 <  SRR19973061.856170/150‑1 (MQ=60)
                                                                                                                                      TACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCACCGATCCACCTTTGTTCAGATAGGCGATCAGATCGGTCACATC                 >  SRR19973061.149637/1‑150 (MQ=60)
                                                                                                                                        CCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCACCGATCCACCTTTGTTCAGATAGGCGATCAGATCGGTCACATCCT               <  SRR19973061.649900/150‑1 (MQ=60)
                                                                                                                                         CGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCACCGATCCACCTTTGTTCAGACAGGCGATCAGATCGGTCACATCCTG              >  SRR19973061.1174287/1‑150 (MQ=60)
                                                                                                                                           ACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCACCGATCCACCTTTGTTCAGATAGGCGATCAGATCGGTCACATCCTGCT            <  SRR19973061.764659/150‑1 (MQ=60)
                                                                                                                                           ACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCACCGATCCACCTTTGTTCAGATAGGCGATCAGATCGGTCACATCCTGCT            <  SRR19973061.3680953/150‑1 (MQ=60)
                                                                                                                                            CTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCACCGATCCACCTTTGTTCAGATAGGCGATCAGATCGGTCACATCCTGCTG           >  SRR19973061.1984928/1‑150 (MQ=60)
                                                                                                                                              TGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCACCGATCCACCTTTGTTCAGATAGGCGATCAGATCGGTCACATCCTGCTGAG         >  SRR19973061.2091885/1‑150 (MQ=60)
                                                                                                                                                TTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCACCGATCCACCTTTGTTCAGATAGGCGATCAGATCGGTCACATCCTGCTGAGTC       <  SRR19973061.248966/150‑1 (MQ=60)
                                                                                                                                                 TCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCACCGATCCACCTTTGTTCAGATAGGCGATCAGATCGGTCACATCCTGCTGAGTCA      >  SRR19973061.3399983/1‑150 (MQ=60)
                                                                                                                                                 TCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCACCGATCCACCTTTGTTCAGATAGGCGATCAGATCGGTCACATCCTGCTGAGTCA      >  SRR19973061.714465/1‑150 (MQ=60)
                                                                                                                                                     AGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCACCGATCCACCTTTGTTCAGATAGGCGATCAGATCGGTCACATCCTGCTGAGTCAGCTT  <  SRR19973061.1213301/150‑1 (MQ=60)
                                                                                                                                                     AGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCACCGATCCACCTTTGTTCAGATAGGCGATCAGATCGGTCACATCCTGCTGAGTCAGCTT  <  SRR19973061.602720/150‑1 (MQ=60)
                                                                                                                                                     |                                                                                                                                                     
TTAACTTCCCCTGTCTTACTATCGATGGTGTACGGCAGCGCACCATCTACGGCAGGATAACGTTCATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGTGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCACCGATCCACCTTTGTTCAGATAGGCGATCAGATCGGTCACATCCTGCTGAGTCAGCTT  >  CP009273/3110064‑3110362

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑