Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F534 I1 R1 1235 230.2 7611948 95.1% 7238962 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009273 3,116,446 A→C L240R (CTG→CGG)  glcB ← malate synthase G

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,116,4460AC99.6% 941.5 / ‑4.8 242L240R (CTG→CGG) glcBmalate synthase G
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base C (112/129);  minor base T (1/0);  total (113/129)
Fisher's exact test for biased strand distribution p-value = 4.67e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.86e-01

Only 100 of 242 total aligned reads displayed.

TGCGGTACAGCAGGATTTTATCTTCCGCATCAACCGCCGCGACCGAATCTTCGCAGTCGAGAATGGTACTGATAGCAGCTTCGACGATAACATCGTTGATGTGCGCCGGATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCAGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATGCAGGTCGGCGCAGCGGCATCGCCACGGTAACCGACAAACTGTGCTGGAGTACGTAACGTGGTTTCTTTACCATTTTTCAACTGGATGCGTAATTGTTTATCAACCACCTTA  >  CP009273/3116300‑3116592
                                                                                                                                                  |                                                                                                                                                  
tGCGGTACAGCAGGATTTTATCTTCCGCATCAACCGCCGCGACCGAATCTTCGCAGTCGAGAATGGTACTGATAGCAGCTTCGACGATAACATCGTTGATGTGCGCCGGATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCCGCt                                                                                                                                                 <  2:2437540/150‑1 (MQ=255)
   ggTACAGCAGGATTTTATCTTCCGCATCAACCGCCGCGACCGAATCTTCGCAGTCGAGAATGGTACTGATAGCAGCTTCGACGATAACATCGTTGATGTGCGCCGGATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCCGCTCaa                                                                                                                                              <  2:2887464/150‑1 (MQ=255)
    gTACAGCAGGATTTTATCTTCCGCATCAACCGCCGCGACCGAATCTTCGCAGTCGAGAATGGTACTGATAGCAGCTTCGACGATAACATCGTTGATGTGCGCCGGATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCCGCTCAat                                                                                                                                             >  2:2334521/1‑150 (MQ=255)
       cagcagGATTTTATCTTCCGCATCAACCGCCGCGACCGAATCTTCGCAGTCGAGAATGGTACTGATAGCAGCTTCGACGATAACATCGTTGATGTGCGCCGGATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCCGCTCAATATg                                                                                                                                          >  1:2106677/1‑150 (MQ=255)
       cagcagGATTTTATCTTCCGCATCAACCGCCGCGACCGAATCTTCGCAGTCGAGAATGGTACTGATAGCAGCTTCGACGATAACATCGTTGATGTGCGCCGGATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCCGCTCAATATg                                                                                                                                          >  2:1149130/1‑150 (MQ=255)
       cagcagGATTTTATCTTCCGCATCAACCGCCGCGACCGAATCTTCGCAGTCGAGAATGGTACTGATAGCAGCTTCGACGATAACATCGTTGATGTGCGCCGGATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCCGCTCAATATg                                                                                                                                          >  1:1361173/1‑150 (MQ=255)
       cagcagGATTTTATCTTCCGCATCAACCGCCGCGACCGAATCTTCGCAGTCGAGAATGGTACTGATAGCAGCTTCGACGATAACATCGTTGATGTGCGCCGGATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCCGCTCAATATg                                                                                                                                          <  2:654286/150‑1 (MQ=255)
        agcagGATTTTATCTTCCGCATCAACCGCCGCGACCGAATCTTCGCAGTCGAGAATGGTACTGATAGCAGCTTCGACGATAACATCGTTGATGTGCGCCGGATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCCGCTCAATATGc                                                                                                                                         >  1:1850909/1‑150 (MQ=255)
         gcagGATTTTATCTTCCGCATCAACCGCCGCGACCGAATCTTCGCAGTCGAGAATGGTACTGATAGCAGCTTCGACGATAACATCGTTGATGTGCGCCGGATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCCGCTCAATATGCa                                                                                                                                        <  2:1735599/150‑1 (MQ=255)
         gcagGATTTTATCTTCCGCATCAACCGCCGCGACCGAATCTTCGCAGTCGAGAATGGTACTGATAGCAGCTTCGACGATAACATCGTTGATGTGCGCCGGATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCCGCTCAATATGCa                                                                                                                                        <  2:3629689/150‑1 (MQ=255)
          cagGATTTTATCTTCCGCATCAACCGCCGCGACCGAATCTTCGCAGTCGAGAATGGTACTGATAGCAGCTTCGACGATAACATCGTTGATGTGCGCCGGATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCCGCTCAATATGCAg                                                                                                                                       <  1:863927/150‑1 (MQ=255)
          cagGATTTTATCTTCCGCATCAACCGCCGCGACCGAATCTTCGCAGTCGAGAATGGTACTGATAGCAGCTTCGACGATAACATCGTTGATGTGCGCCGGATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCCGCTCAATATGCAg                                                                                                                                       >  2:1105974/1‑150 (MQ=255)
           agGATTTTATCTTCCGCATCAACCGCCGCGACCGAATCTTCGCAGTCGAGAATGGTACTGATAGCAGCTTCGACGATAACATCGTTGATGTGCGCCGGATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCCGCTCAATATGCAgg                                                                                                                                      >  2:143424/1‑150 (MQ=255)
            ggATTTTATCTTCCGCATCAACCGCCGCGACCGAATCTTCGCAGTCGAGAATGGTACTGATAGCAGCTTCGACGATAACATCGTTGATGTGCGCCGGATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCCGCTCAATATGCAGGc                                                                                                                                     >  2:2495219/1‑150 (MQ=255)
                tttATCTTCCGCATCAACCGCCGCGACCGAATCTTCGCAGTCGAGAATGGTACTGATAGCAGCTTCGACGATAACATCGTTGATGTGCGCCGGATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCCGCTCAATATGCAGGCCGtt                                                                                                                                 <  1:2194015/150‑1 (MQ=255)
                tttATCTTCCGCATCAACCGCCGCGACCGAATCTTCGCAGTCGAGAATGGTACTGATAGCAGCTTCGACGATAACATCGTTGATGTGCGCCGGATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCCGCTCAATATGCAGGCCGtt                                                                                                                                 >  1:2906146/1‑150 (MQ=255)
                tttATCTTCCGCATCAACCGCCGCGACCGAATCTTCGCAGTCGAGAATGGTACTGATAGCAGCTTCGACGATAACATCGTTGATGTGCGCAGGATCGTCTTTGCCAATCCGCCCATTGGCATATATTTGCCGCTCAATATGCAGGCCGtt                                                                                                                                 <  1:3713503/150‑1 (MQ=255)
                  tATCTTCCGCATCAACCGCCGCGACCGAATCTTCGCAGTCGAGAATGGTACTGATAGCAGCTTCGACGATAACATCGTTGATGTGCGCCGGATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCCGCTCAATATGCAGGCCGTTAt                                                                                                                               >  2:2359789/1‑150 (MQ=255)
                  tATCTTCCGCATCAACCGCCGCGACCGAATCTTCGCAGTCGAGAATGGTACTGATAGCAGCTTCGACGATAACATCGTTGATGTGCGCCGGATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCCGCTCAATATGCAGGCCGTTAt                                                                                                                               >  2:3068201/1‑150 (MQ=255)
                   aTCGTCCGCATCAACCGCCGCGACCGAATCTTCGCAGTCGAGAATGGTACTGATAGCAGCTTCGACGATAACATCGTTGATGTGCGCCGGATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCCGCTCAATATGCATGCCGTTAtt                                                                                                                              <  1:3794228/150‑1 (MQ=255)
                       tCCGCATCAACCGCCGCGACCGAATCTTCGCAGTCGAGAATGGTACTGATAGCAGCTTCGACGATAACATCGTTGATGTGCGCCGGATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCCGCTCAATATGCAGGCCGTTATTTTTc                                                                                                                          <  2:11023/150‑1 (MQ=255)
                       tCCGCATCAACCGCCGCGACCGAATCTTCGCAGTCGAGAATGGTACTGATAGCAGCTTCGACGATAACATCGTTGATGTGCGCCGGATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCCGCTCAATATGCAGGCCGTTATTTTTc                                                                                                                          <  2:936495/150‑1 (MQ=255)
                             tCAACCGCCGCGACCGAATCTTCGCAGTCGAGAATGGTACTGATAGCAGCTTCGACGATAACATCGTTGATGTGCGCCGGATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCCGCTCAATATGCAGGCCGTTATTTTTCAGCaaa                                                                                                                    <  2:1896531/150‑1 (MQ=255)
                               aaCCGCCGCGACCGAATCTTCGCAGTCGAGTATGGTACTGATAGCAGCTTCGACGATAACATCGTTGATGTGCGCCGGATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCCGCTCAATATGGAGGCCGTTATTTTTCAGCAAAAt                                                                                                                  <  2:2186951/150‑1 (MQ=255)
                                   gccgcGACCGAATCTTCGCAGTCGAGAATGGTACTGATAGCAGCTTCGACGATAACATCGTTGATGTGCGCCGGATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCCGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATGCAg                                                                                                              >  1:2814283/1‑150 (MQ=255)
                                    ccgcGACCGAATCTTCGCAGTCGAGAATGGTACTGATAGCAGCTTCGACGATAACATCGTTGATGTGCGCCGGATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCCGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATGCAgg                                                                                                             <  1:2042820/150‑1 (MQ=255)
                                     cgcgACCGAATCTTCGCAGTCGAGAATGGTACTGATAGCAGCTTCGACGATAACATCGTTGATGTGCGCCGGATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCCGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATGCAGGt                                                                                                            >  1:1240194/1‑150 (MQ=255)
                                     cgcgACCGAATCTTCGCAGTCGAGAATGGTACTGATAGCAGCTTCGACGATAACATCGTTGATGTGCGCCGGATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCCGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATGCAGGt                                                                                                            <  1:2558202/150‑1 (MQ=255)
                                        gACCGAATCTTCGCAGTCGAGAATGGTACTGATAGCAGCTTCGACGATAACATCGTTGATGTGCGCCGGATCGTCTTTGACAATCCGCCCATTGGCATCGATTTGCCGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATTCAGGTctg                                                                                                         >  2:2285651/1‑148 (MQ=255)
                                           cGAATCTTCGCAGTCGAGAATGGTACTGATAGCAGCTTCGACGATAACATCGTTGATGTGCGCCGGATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCCGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATGCAGGTCGgcgc                                                                                                      >  1:3548288/1‑150 (MQ=255)
                                            gAATCTTCGCAGTCGAGAATGGTACTGATAGCAGCTTCGACGATAACATCGTTGATGTGCGCCGGATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCCGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATGCAGGTCGGCGCa                                                                                                     >  1:1596063/1‑150 (MQ=255)
                                            gAATCTTCGCAGTCGAGAATGGTACTGATAGCAGCTTCGACGATAACATCGTTGATGTGCGCCGGATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCCGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATGCAGGTCGGCGCa                                                                                                     >  1:589214/1‑150 (MQ=255)
                                            gAATCTTCGCAGTCGAGAATGGTACTGATAGCAGCTTCGACGATAACATCGTTGATGTGCGCCGGATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCCGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATGCAGGTCGGCGCa                                                                                                     <  2:120222/150‑1 (MQ=255)
                                              aTCTTCGCAGTCGAGAATGGTACTGATAGCAGCTTCGACGATAACATCGTTGATGTGCGCCGGATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCCGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATGCAGGTCGGCGCAGc                                                                                                   >  1:2376274/1‑150 (MQ=255)
                                              aTCTTCGCAGTCGAGAATGGTACTGATAGCAGCTTCGACGATAACATCGTTGATGTGCGCCGGATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCCGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATGCAGGTCGGCGCAGc                                                                                                   >  1:1841574/1‑150 (MQ=255)
                                                cTTCGCAGTCGAGAATGGTACTGATAGCAGCTTCGACGATAACATCGTTGATGTGCGCCGGATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCCGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATGCAGGTCGGCGCAGCgg                                                                                                 <  2:2221683/150‑1 (MQ=255)
                                                  tCGCAGTCGAGAATGGTACTGATAGCAGCTTCGACGATAACATCGTTGATGTGCGCCGGATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCCGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATGCAGGTCGGCGCAGCGGCa                                                                                               >  2:3394088/1‑150 (MQ=255)
                                                   cGCAGTCGAGAATGGTACTGATAGCAGCTTCGACGATAACATCGTTGATGTGCGCCGGATCGTCTTTGCCAATCCGCCCATTGGCATCTATTTGCCGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATGCAGGTCGGCGCAGCGGCAt                                                                                              >  1:1499872/1‑150 (MQ=255)
                                                     cAGTCGAGAATGGTACTGATAGCAGCTTCGACGATAACATCGTTGATGTGCGCCGGATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCCGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATGCAGGTCGGCGCAGCGGCATCg                                                                                            <  1:1275020/150‑1 (MQ=255)
                                                      aGTCGAGAATGGTACTGATAGCAGCTTCGACGATAACATCGTTGATGTGCGCCGGATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCCGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATGCAGGTCGGCGCAGCGGCATCGc                                                                                           >  1:2294856/1‑150 (MQ=255)
                                                        tCGAGAATGGTACTGATAGCAGCTTCGACGATAACATCGTTGATGTGCGCCGGATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCCGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATGCAGGTCGGCGCAGCGGCATCGCCa                                                                                         <  2:2132758/150‑1 (MQ=255)
                                                         cGAGAATGGTACTGATAGCAGCTTCGACGATAACATCGTTGATGTGCGCCGGATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCCGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATGCAGGTCGGCGCAGCGGCATCGCCAc                                                                                        <  1:2495982/150‑1 (MQ=255)
                                                            gaATGGTACTGATAGCAGCTTCGACGATAACATCGTTGATGTGCGCCGGATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCCGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATGCAGGTCGGCGCAGCGGCATCGCCACGGt                                                                                     >  2:316002/1‑150 (MQ=255)
                                                             aaTGGTACTGATAGCAGCTTCGACGATAACATCGTTGATGTGCGCCGGATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCCGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATGCAGGTCGGCGCAGCGGCATCGCCACGGTa                                                                                    <  2:505845/150‑1 (MQ=255)
                                                               tGGTACTGATAGCAGCTTCGACGATAACATCGTTGATGTGCGCCGGATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCCGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATGCAGGTCGGCGCAGCGGCATCGCCACGGTAAc                                                                                  <  1:695226/150‑1 (MQ=255)
                                                                ggTACTGATAGCAGCTTCGACGATAACATCGTTGATGTGCGCCGGATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCCGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATGCAGGTCGGCGCAGCGGCATCGCCACGGTAAcc                                                                                 <  2:772133/150‑1 (MQ=255)
                                                                ggTACTGATAGCAGCTTCGACGATAACATCGTTGATGTGCGCCGGATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCCGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATGCAGGTCGGCGCAGCGGCATCGCCACGGTAAcc                                                                                 <  2:3362512/150‑1 (MQ=255)
                                                                 gTACTGATAGCAGCTTCGACGATAACATCGTTGATGTGCGCCGGATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCCGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATGCAGGTCGGCGCAGCGGCATCGCCACGGTAACCg                                                                                <  1:414064/150‑1 (MQ=255)
                                                                      gATAGCAGCTTCGACGATAACATCGTTGATGTGCGCCGGATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCCGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATGCAGGTCGGCGCAGCGGCATCGCCACGGTAACCGACaaa                                                                           <  1:3189446/150‑1 (MQ=255)
                                                                       aTAGCAGCTTCGACGATAACATCGTTGATGTGCGCCGGATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCCGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATGCAGGTCGGCGCAGCGGCATCGCCACGGTAACCGACAAAc                                                                          >  2:2918681/1‑150 (MQ=255)
                                                                         agcagcTTCGACGATAACATCGTTGATGTGCGCCGGATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCCGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATGCAGGTCGGCGCAGCGGCATCGCCACGGTAACCGACAAACtg                                                                        >  2:3499745/1‑150 (MQ=255)
                                                                           cagcTTCGACGATAACATCGTTGATGTGCGCCGGATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCCGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATGCAGGTCGGCGCAGCGGCATCGCCACGGTAACCGACAAACtgtg                                                                      <  1:2580100/150‑1 (MQ=255)
                                                                                tCGACGATAACATCGTTGATGTGCGCCGGATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCCGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATGCAGGTCGGCGCAGCGGCATCGCCACGGTAACCGACAAACTGTGCTGGa                                                                 <  1:2805406/150‑1 (MQ=255)
                                                                                 cgacgaTAACATCGTTGATGTGCGCCGGATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCCGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATGCAGGTCGGCGCAGCGGCATCGCCACGGTAACCGACAAACTGTGCTGGAg                                                                <  2:3307634/150‑1 (MQ=255)
                                                                                  gacgaTAACATCGTTGATGTGCGCCGGATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCCGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATGCAGGTCGGCGCAGCGGCATCGCCACGGTAACCGACAAACTGTGCTGGAGt                                                               <  1:3632926/150‑1 (MQ=255)
                                                                                   acgaTAACATCGTTGATGTGCGCCGGATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCCGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATGCAGGTCGGCGCAGCGGCATCGCCACGGTAACCGACAAACTGTGCTGGAGTa                                                              >  2:1282492/1‑150 (MQ=255)
                                                                                    cgaTAACATCGTTGATGTGCGCCGGATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCCGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATGCAGGTCGGCGCAGCGGCATCGCCACGGTAACCGACAAACTGTGCTGGAGTAc                                                             <  1:429431/150‑1 (MQ=255)
                                                                                     gaTAACATCGTTGATGTGCGCCGGATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCCGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATGCAGGTCGGCGCAGCGGCATCGCCACGGTAACCGACAAACTGTGCTGGAGTACg                                                            <  1:2067998/150‑1 (MQ=255)
                                                                                     gaTAACATCGTTGATGTGCGCCGGATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCCGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATGCAGGTCGGCGCAGCGGCATCGCCACGGTAACCGACAAACTGTGCTGGAGTACg                                                            <  1:1084677/150‑1 (MQ=255)
                                                                                        aaCATCGTTGATGTGCGCCGGATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCCGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATGCAGGTCGGCGCAGCGGCATCGCCACGGTAACCGACAAACTGTGCTGGAGTACGTaa                                                         <  2:1512984/150‑1 (MQ=255)
                                                                                        aaCATCGTTGATGTGCGCCGGATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCCGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATGCAGGTCGGCGCAGCGGCATCGCCACGGTAACCGACAAACTGTGCTGGAGTACGTaa                                                         <  2:589214/150‑1 (MQ=255)
                                                                                           aTCGTTGATGTGCGCCGGATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCCGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATGCAGGTCGGCGCAGCGGCATCGCCACGGTAACCGACAAACTGTGCTGGAGTACGTAACGt                                                      <  2:2568478/150‑1 (MQ=255)
                                                                                           aTCGTTGATGTGCGCCGGATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCCGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATGCAGGTCGGCGCAGCGGCATCGCCACGGTAACCGACAAACTGTGCTGGAGTACGTAACGt                                                      >  2:2020053/1‑150 (MQ=255)
                                                                                            tCGTTGATGTGCGCCGGATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCCGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATGCAGGTCGGCGCAGCGGCATCGCCACGGTAACCGACAAACTGTGCTGGAGTACGTAACGTg                                                     <  2:3751093/150‑1 (MQ=255)
                                                                                             cGTTGATGTGCGCCGGATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCCGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATGCAGGTCGGCGCAGCGGCATCGCCACGGTAACCGACAAACTGTGCTGGAGTACGTAACGTgg                                                    >  1:3367722/1‑150 (MQ=255)
                                                                                             cGTTGATGTGCGCCGGATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCCGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATGCAGGTCGGCGCAGCGGCATCGCCACGGTAACCGACAAACTGTGCTGGAGTACGTAACGTgg                                                    >  2:2667477/1‑150 (MQ=255)
                                                                                             cGTTGATGTGCGCCGGATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCCGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATGCAGGTCGGCGCAGCGGCATCGCCACGGTAACCGACAAACTGTGCTGGAGTACGTAACGTgg                                                    >  2:762581/1‑150 (MQ=255)
                                                                                                tGATGTGCGCCGGATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCCGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATGCAGGTCGACGCCTCGGCATCGCCACTGAAACCGACAAACTGTGCTGGAGTACGTAACGTGGttt                                                 >  1:3005818/1‑150 (MQ=255)
                                                                                                  aTGTGCGCCGGATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCCGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATGCAGGTCGGCGCAGCGGCATCGCCACGGTAACCGACAAACTGTGCTGGAGTACGTAACGTGGTTTCt                                               <  1:2359789/150‑1 (MQ=255)
                                                                                                   tgtgCGCCGGATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCCGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATGCAGGTCGGCGCAGCGGCATCGCCACGGTAACCGACAAACTGTGCTGGAGTACGTAACGTGGTTTCtt                                              <  2:3723113/150‑1 (MQ=255)
                                                                                                     tgCGCCGGATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCCGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATGCAGGTCGGCGCAGCGGCATCGCCACGGTAACCGACAAACTGTGCTGGAGTACGTAACGTGGTTTCTTTa                                            >  1:3782033/1‑150 (MQ=255)
                                                                                                     tgCGCCGGATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCCGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATGCAGGTCGGCGCAGCGGCATCGCCACGGTAACCGACAAACTGTGCTGGAGTACGTAACGTGGTTTCTTTa                                            <  2:1430941/150‑1 (MQ=255)
                                                                                                       cgcCGGATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCCGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATGCAGGTCGGCGCAGCGGCATCGCCACGGTAACCGACAAACTGTGCTGGAGTACGTAACGTGGTTTCTTTAcc                                          <  2:87591/150‑1 (MQ=255)
                                                                                                       cgcCGGATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCCGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATGCAGGTCGGCGCAGCGGCATCGCCACGGTAACCGACAAACTGTGCTGGAGTACGTAACGTGGTTTCTTTAcc                                          >  2:1659158/1‑150 (MQ=255)
                                                                                                           ggATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCCGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATGCAGGTCGGCGCAGCGGCATCGCCACGGTAACCGACAAACTGTGCTGGAGTACGTAACGTGGTTTCTTTACCAttt                                      >  1:1700790/1‑150 (MQ=255)
                                                                                                            gATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCCGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATGCAGGTCGGCGCAGCGGCATCGCCACGGTAACCGACAAACTGTGCTGGAGTACGTAACGTGGTTTCTTTACCAtttt                                     >  1:3254429/1‑150 (MQ=255)
                                                                                                               cGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCCGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATGCAGGTCGGCGCAGCGGCATCGCCACGGTAACCGACAAACTGTGCTGGAGTACGTAACGTGGTTTCTTTACCATTTTTCa                                  >  2:3410745/1‑150 (MQ=255)
                                                                                                               cGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCCGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATGCAGGTCGGCGCAGCGGCATCGCCACGGTAACCGACAAACTGTGCTGGAGTACGTAACGTGGTTTCTTTACCATTTTTCa                                  >  1:509134/1‑150 (MQ=255)
                                                                                                                gtatttGCCAATCCGCCCATTGGCATCGATTTGCCGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATGCAGGTCGGCGCAGCGGCATCGCCACGGTAACCGACAAACTGTGCTGGAGTACGTAACGTGGTTTCTTTACCATTTTTCaa                                 <  1:1784306/147‑1 (MQ=255)
                                                                                                                 tCTTTGCCAATCCGCCCATTGGCATCGATTTGCCGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATGCAGGTCGGCGCAGCGGCATCGCCACGGTAACCGACAAACTGTGCTGGAGTACGTAACGTGGTTTCTTTACCATTTTTCAAc                                <  2:1841574/150‑1 (MQ=255)
                                                                                                                  cTTTGCCAATCCGCCCATTGGCATCGATTTGCCGCTCAATATTCAGGCCGTTATTTTTCAGCAAAATGCAGGTCGGCGCAGCGGCATCGCCACGGTAACCGACAAACTGTGCTGGAGTACGTAACGTGGTTTCTTTACCATTTTTCAACt                               <  2:2985996/150‑1 (MQ=255)
                                                                                                                  cTTTGCCAATCCGCCCATTGGCATCGATTTGCCGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATGCAGGTCGGCGCAGCGGCATCGCCACGGTAACCGACAAACTGTGCTGGAGTACGTAACGTGGTTTCTTTACCATTTTTCAACt                               >  2:3176899/1‑150 (MQ=255)
                                                                                                                    ttGCCAATCCGCCCATTGGCATCGATTTGCCGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATGCAGGTCGGCGCAGCGGCATCGCCACGGTAACCGACAAACTGTGCTGGAGTACGTAACGTGGTTTCTTTACCATTTTTCAACTgg                             <  1:3447286/150‑1 (MQ=255)
                                                                                                                     tGCCAATCCGCCCATTGGCATCGATTTGCCGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATGCAGGTCGGCGCAGCGGCATCGCCACGGTAACCGACAAACTGTGCTGGAGTACGTAACGTGGTTTCTTTACCATTTTTCAACTGGa                            >  1:907872/1‑150 (MQ=255)
                                                                                                                      gCCAATCCGCCCATTGGCATCGATTTGCCGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATGCAGGTCGGCGCAGCGGCATCGCCACGGTAACCGACAAACTGTGCTGGAGTACGTAACGTGGTTTCTTTACCATTTTTCAACTGGAt                           <  2:3548221/150‑1 (MQ=255)
                                                                                                                       ccAATCCGCCCATTGGCATCGATTTGCCGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATGCAGGTCGGCGCAGCGGCATCGCCACGGTAACCGACAAACTGTGCTGGAGTACGTAACGTGGTTTCTTTACCATTTTTCAACTGGATg                          <  2:1596063/150‑1 (MQ=255)
                                                                                                                        cAATCCGCCCATTGGCATCGATTTGCCGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATGCAGGTCGGCGCAGCGGCATCGCCACGGTAACCGACAAACTGTGCTGGAGTACGTAACGTGGTTTCTTTACCATTTTTCAACTGGATGc                         <  2:1291129/150‑1 (MQ=255)
                                                                                                                           tCCGCCCATTGGCATCGATTTGCCGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATGCAGGTCGGCGCAGCGGCATCGCCACGGTAACCGACAAACTGTGCTGGAGTACGTAACGTGGTTTCTTTACCATTTTTCAACTGGATGCGTa                      <  1:3575589/150‑1 (MQ=255)
                                                                                                                           tCCGCCCATTGGCATCGATTTGCCGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATGCAGGTCGGCGCAGCGGCATCGCCACGGTAACCGACAAACTGTGCTGGAGTACGTAACGTGGTTTCTTTACCATTTTTCAACTGGATGCGTa                      <  2:156200/150‑1 (MQ=255)
                                                                                                                           tCCGCCCATTGGCATCGATTTGCCGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATGCAGGTCGGCGCAGCGGCATCGCCACGGTAACCGACAAACTGTGCTGGAGTACGTAACGTGGTTTCTTTACCATTTTTCAACTGGATGCGTa                      <  2:2677987/150‑1 (MQ=255)
                                                                                                                             cGCCCATTGGCATCGATTTGCCGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATGCAGGTCGGCGCAGCGGCATCGCCACGGTAACCGACAAACTGTGCTGGAGTACGTAACGTGGTTTCTTTACCATTTTTCAACTGGATGCGTAAt                    >  2:281001/1‑150 (MQ=255)
                                                                                                                                 cATTGGCATCGATTTGCCGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATGCAGGTCGGCGCAGCGGCATCGCCACGGTAACCGACAAACTGTGCTGGAGTACGTAACGTGGTTTCTTTACCATTTTTCAACTGGATGCGTAATTGtt                <  1:3068201/150‑1 (MQ=255)
                                                                                                                                   ttGGCATCGATTTGCCGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATGCAGGTCGGCGCAGCGGCATCGCCACGGTAACCGACAAACTGTGCTGGAGTACGTAACGTGGTTTCTTTACCATTTTTCAACTGGATGCGTAATTGTTTa              <  2:24326/150‑1 (MQ=255)
                                                                                                                                   ttGGCATCGATTTGCCGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATGCAGGTCGGCGCAGCGGCATCGCCACGGTAACCGACAAACTGTGCTGGAGTACGTAACGTGGTTTCTTTACCATTTTTCAACTGGATGCGTAATTGTTTa              >  2:1791015/1‑150 (MQ=255)
                                                                                                                                     ggCATCGATTTGCCGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATGCAGGTCGGCGCAGCGGCATCGCCACGGTAACCGACAAACTGTGCTGGAGTACGTAACGTGGTTTCTTTACCATTTTTCAACTGGATGCGTAATTGTTTATc            >  2:2656094/1‑150 (MQ=255)
                                                                                                                                      gCATCGATTTGCCGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATGCAGGTCGGCGCAGCGGCATCGCCACGGTAACCGACAAACTGTGCTGGAGTACGTAACGTGGTTTCTTTACCATTTTTCAACTGGATGCGTAATTGTTTATCa           <  1:3746160/150‑1 (MQ=255)
                                                                                                                                       cATCGATTTGCCGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATGCAGGTCGGCGCAGCGGCATCGCCACGGTAACCGACAAACTGTGCTGGAGTACGTAACGTGGTTTCTTTACCATTTTTCAACTGGATGCGTAATTGTTTATCaa          >  1:545312/1‑150 (MQ=255)
                                                                                                                                           gATTTGCCGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATGCAGGTCGGCGCAGCGGCATCGCCACGGTAACCGACAAACTGTGCTGGAGTACGTAACGTGGTTTCTTTACCATTTTTCAACTGGATGCGTAATTGTTTATCAaccac      >  2:1290667/1‑150 (MQ=255)
                                                                                                                                              ttGCCGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATGCAGGTCGGCGCAGCGGCATCGCCACGGTAACCGACAAACTGTGCTGGAGTACGTAACGTGGTTTCTTTACCATTTTTCAACTGGATGCGTAATTGTTTATCAACCACCtt   <  1:143424/150‑1 (MQ=255)
                                                                                                                                               tGCCGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATGCAGGTCGGCGCAGCGGCATCGCCACGGTAACCGACAAACTGTGCTGGAGTACGTAACGTGGTTTCTTTACCATTTTTCAACTGGATGCGTAATTGTTTATCAACCACCTTa  >  1:110604/1‑150 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
TGCGGTACAGCAGGATTTTATCTTCCGCATCAACCGCCGCGACCGAATCTTCGCAGTCGAGAATGGTACTGATAGCAGCTTCGACGATAACATCGTTGATGTGCGCCGGATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCAGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATGCAGGTCGGCGCAGCGGCATCGCCACGGTAACCGACAAACTGTGCTGGAGTACGTAACGTGGTTTCTTTACCATTTTTCAACTGGATGCGTAATTGTTTATCAACCACCTTA  >  CP009273/3116300‑3116592

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GGTTGCGGTACAGCAGGATTTTATCTTCCGCATCAACCGCCGCGACCGAATCTTCGCAGTCGAGAATGGTACTGATAGCAGCTTCGACGATAACATCGTTGATGTGCGCCGGATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCAGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATGCAGGTCGGCGCAGCGGCATCGCCACGGTAACCGACAAACTGTGCTGGAGTACGTAACGTGGTTTCTTTACCATTTTTCAACTGGATGCGTAATTGTTTATCAACCACCTTAAAC  >  CP009273/3116297‑3116595
                                                                                                                                                     |                                                                                                                                                     
GGTTGCGGTACAGCAGGATTTTATCTTCCGCATCAACCGCCGCGACCGAATCTTCGCAGTCGAGAATGGTACTGATAGCAGCTTCGACGATAACATCGTTGATGTGCGCCGGATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCC                                                                                                                                                       <  SRR19973061.905588/150‑1 (MQ=60)
GGTTGCGGTACAGCAGGATTTTATCTTCCGCATCAACCGCCGCGACCGAATCTTCGCAGTCGAGAATGGTACTGATAGCAGCTTCGACGATAACATCGTTGATGTGCGCCGGATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCC                                                                                                                                                       <  SRR19973061.1863831/150‑1 (MQ=60)
 GTTGCGGTACAGCAGGATTTTATCTTCCGCATCAACCGCCGCGACCGAATCTTCGCAGTCGAGAATGGTACTGATAGCAGCTTCGACGATAACATCGTTGATGTGCGCCGGATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCCG                                                                                                                                                      <  SRR19973061.3616769/150‑1 (MQ=60)
 GTTGCGGTACAGCAGGATTTTATCTTCCGCATCAACCGCCGCGACCGAATCTTCGCAGTCGAGAATGGTACTGATAGCAGCTTCGACGATAACATCGTTGATGTGCGCCGGATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCCG                                                                                                                                                      <  SRR19973061.3617145/150‑1 (MQ=60)
 GTTGCGGTACAGCAGGATTTTATCTTCCGCATCAACCGCCGCGACCGAATCTTCGCAGTCGAGAATGGTACTGATAGCAGCTTCGACGATAACATCGTTGATGTGCGCCGGATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCCG                                                                                                                                                      <  SRR19973061.1686001/150‑1 (MQ=60)
          CAGCAGGATTTTATCTTCCGCATCAACCGCCGCGACCGAATCTTCGCAGTCGAGAATGGTACTGATAGCAGCTTCGACGATAACATCGTTGATGTGCGCCGGATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCCGCTCAATATG                                                                                                                                             >  SRR19973061.1364607/1‑150 (MQ=60)
          CAGCAGGATTTTATCTTCCGCATCAACCGCCGCGACCGAATCTTCGCAGTCGAGAATGGTACTGATAGCAGCTTCGACGATAACATCGTTGATGTGCGCCGGATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCCGCTCAATATG                                                                                                                                             >  SRR19973061.2111964/1‑150 (MQ=60)
           AGCAGGATTTTATCTTCCGCATCAACCGCCGCGACCGAATCTTCGCAGTCGAGAATGGTACTGATAGCAGCTTCGACGATAACATCGTTGATGTGCGCCGGATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCCGCTCAATATGC                                                                                                                                            >  SRR19973061.1855541/1‑150 (MQ=60)
           AGCAGGATTTTATCTTCCGCATCAACCGCCGCGACCGAATCTTCGCAGTCGAGAATGGTACTGATAGCAGCTTCGACGATAACATCGTTGATGTGCGCCGGATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCCGCTCAATATGC                                                                                                                                            >  SRR19973061.1855593/1‑150 (MQ=60)
             CAGGATTTTATCTTCCGCATCAACCGCCGCGACCGAATCTTCGCAGTCGAGAATGGTACTGATAGCAGCTTCGACGATAACATCGTTGATGTGCGCCGGATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCCGCTCAATATGCAG                                                                                                                                          <  SRR19973061.866166/150‑1 (MQ=60)
              AGGATTTTATCTTCCGCATCAACCGCCGCGACCGAATCTTCGCAGTCGAGAATGGTACTGATAGCAGCTTCGACGATAACATCGTTGATGTGCGCCGGATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCCGCTCAATATGCAGG                                                                                                                                         <  SRR19973061.2316779/150‑1 (MQ=60)
               GGATTTTATCTTCCGCATCAACCGCCGCGACCGAATCTTCGCAGTCGAGAATGGTACTGATAGCAGCTTCGACGATAACATCGTTGATGTGCGCCGGATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCCGCTCAATATGCAGGC                                                                                                                                        <  SRR19973061.1244314/150‑1 (MQ=60)
               GGATTGTATCTTCCGCATCAACCGCCGCGACCGAATCTTCGCAGTCGAGAATGGTACTGATAGCAGCTTCGACGATAACATCGTTGATGTGCGCCGGATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCCGCTCAATATGCAGGC                                                                                                                                        <  SRR19973061.1244321/150‑1 (MQ=60)
                GATTTTATCTTCCGCATCAACCGCCGCGACCGAATCTTCGCAGTCGAGAATGGTACTGATAGCAGCTTCGACGATAACATCGTTGATGTGCGCCGGATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCCGCTCAATATGCAGGCC                                                                                                                                       >  SRR19973061.2540163/1‑150 (MQ=60)
                GATTTTATCTTCCGCATCAACCGCCGCGACCGAATCTTCGCAGTCGAGAATGGTACTGATAGCAGCTTCGACGATAACATCGTTGATGTGCGCCGGATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCCGCTCAATATGCAGGCC                                                                                                                                       <  SRR19973061.3748443/150‑1 (MQ=60)
                   TTTATCTTCCGCATCAACCGCCGCGACCGAATCTTCGCAGTCGAGAATGGTACTGATAGCAGCTTCGACGATAACATCGTTGATGTGCGCCGGATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCCGCTCAATATGCAGGCCGTT                                                                                                                                    <  SRR19973061.3721253/150‑1 (MQ=60)
                   TTTATCTTCCGCATCAACCGCCGCGACCGAATCTTCGCAGTCGAGAATGGTACTGATAGCAGCTTCGACGATAACATCGTTGATGTGCGCCGGATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCCGCTCAATATGCAGGCCGTT                                                                                                                                    <  SRR19973061.2199529/150‑1 (MQ=60)
                   TTTATCTTCCGCATCAACCGCCGCGACCGAATCTTCGCAGTCGAGAATGGTACTGATAGCAGCTTCGACGATAACATCGTTGATGTGCGCCGGATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCCGCTCAATATGCAGGCCGTT                                                                                                                                    >  SRR19973061.2913604/1‑150 (MQ=60)
                   TTTATCTTCCGCATCAACCGCCGCGACCGAATCTTCGCAGTCGAGAATGGTACTGATAGCAGCTTCGACGATAACATCGTTGATGTGCGCAGGATCGTCTTTGCCAATCCGCCCATTGGCATATATTTGCCGCTCAATATGCAGGCCGTT                                                                                                                                    <  SRR19973061.3722995/150‑1 (MQ=60)
                    TTATCTTCCGCATCAACCGCCGCGACCGAATCTTCGCAGTCGAGAATGGTACTGATAGCAGCTTCGACGATAACATCGTTGATGTGCGCCGGATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCCGCTCAATATACAGGCCGTTA                                                                                                                                   >  SRR19973061.2993698/1‑150 (MQ=60)
                      ATCTTCCGCATCAACCGCCGCGACCGAATCTTCGCAGTCGAGAATGGTACTGATAGCAGCTTCGACGATAACATCGTTGATGTGCGCCGGATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCCGCTCAATATGCAGGCCGTTATT                                                                                                                                 <  SRR19973061.3575168/150‑1 (MQ=60)
                      ATCGTCCGCATCAACCGCCGCGACCGAATCTTCGCAGTCGAGAATGGTACTGATAGCAGCTTCGACGATAACATCGTTGATGTGCGCCGGATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCCGCTCAATATGCATGCCGTTATT                                                                                                                                 <  SRR19973061.3803898/150‑1 (MQ=60)
                            CGCATCAACCGCCGCGACCGAATCTTCGCAGTCGAGAATGGTACTGATAGCAGCTTCGACGATAACATCGTTGATGTGCGCCGGATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCCGCTCAATATGCAGGCCGTTATTTTTCAG                                                                                                                           <  SRR19973061.3291730/150‑1 (MQ=60)
                            CGCATCAACCGCCGCGACCGAATCTTCGCAGTCGAGAATGGTACTGATAGCAGCTTCGACGATAACATCGTTGATGTGCGCCGGATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCCGCTCAATATGCAGGCCGTTATTTTTCAG                                                                                                                           <  SRR19973061.3292313/150‑1 (MQ=60)
                                 CAACCGCCGCGACCGAATCTTCGCAGTCGAGAATGGTACTGATAGCAGCTTCGACGATAACATCGTTGATGTGCGCCGGATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCCGCTCAATATGCAGGCCGTTATTTTTCAGCAAAA                                                                                                                      >  SRR19973061.2092769/1‑150 (MQ=60)
                                  AACCGCCGCGACCGAATCTTCGCAGTCGAGAATGGTACTGATAGCAGCTTCGACGATAACATCGTTGATGTGCGCCGGATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCCGCTCAATATGCAGGCCGTTATTTTTCAGCAAAAT                                                                                                                     >  SRR19973061.694518/1‑150 (MQ=60)
                                   ACCGCCGCGACCGAATCTTCGCAGTCGAGAATGGTACTGATAGCAGCTTCGACGATAACATCGTTGATGTGCGCCGGATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCTGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATG                                                                                                                    >  SRR19973061.3763387/1‑150 (MQ=60)
                                     CGCCGCGACCGAATCTTCGCAGTCGAGAATGGTACTGATAGCAGCTTCGACGATAACATCGTTGATGTGCGCCGGATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCCGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATGCA                                                                                                                  <  SRR19973061.1482945/150‑1 (MQ=60)
                                      GCCGCGACCGAATCTTCGCAGTCGAGAATGGTACTGATAGCAGCTTCGACGATAACATCGTTGATGTGCGCCGGATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCCGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATGCAG                                                                                                                 >  SRR19973061.2821507/1‑150 (MQ=60)
                                      GCCGCGACCGAATCTTCGCAGTCGAGAATGGTACTGATAGCAGCTTCGACGATAACATCGTTGATGTGCGCCGGATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCCGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATGCAG                                                                                                                 <  SRR19973061.2820224/150‑1 (MQ=60)
                                       CCGCGACCGAATCTTCGCAGTCGAGAATGGTACTGATAGCAGCTTCGACGATAACATCGTTGATGTGCGCCGGATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCCGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATGCAGG                                                                                                                <  SRR19973061.2743785/150‑1 (MQ=60)
                                       CCGCGACCGAATCTTCGCAGTCGAGAATGGTACTGATAGCAGCTTCGACGATAACATCGTTGATGTGCGCCGGATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCCGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATGCAGG                                                                                                                <  SRR19973061.2047953/150‑1 (MQ=60)
                                       CCGCGACCGAATCTTCGCAGTCGAGAATGGTACTGATAGCAGCTTCGACGATAACATCGTTGATGTGCGCCGGATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCCGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATGCAGG                                                                                                                <  SRR19973061.2437576/150‑1 (MQ=60)
                                        CGCGACCGAATCTTCGCAGTCGAGAATGGTACTGATAGCAGCTTCGACGATAACATCGTTGATGTGCGCCGGATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCCGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATGCAGGT                                                                                                               >  SRR19973061.1243273/1‑150 (MQ=60)
                                        CGCGACCGAATCTTCGCAGTCGAGAATGGTACTGATAGCAGCTTCGACGATAACATCGTTGATGTGCGCCGGATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCCGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATGCAGGT                                                                                                               <  SRR19973061.2564731/150‑1 (MQ=60)
                                           GACCGAATCTTCGCAGTCGAGAATGGTACTGATAGCAGCTTCGACGATAACATCGTTGATNTGCGCCGGANCGTCTNNGCCAANCCGCCCATNGGCATCGATTTGCCGCTCAATATGCANGCCGTTATTTTTNAGCAAAATGCAGGTCGG                                                                                                            >  SRR19973061.3649210/1‑150 (MQ=60)
                                           GACCGAATCTTCGCAGTCGAGAATGGTACTGATAGCAGCTTCGACGATAACATCGTTGATGTGCGCCGGATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCCGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATGCAGGTCGG                                                                                                            >  SRR19973061.820700/1‑150 (MQ=60)
                                           GACCGAATCTTCGCAGTCGAGAATGGTACTGATAGCAGCTTCGACGATAACATCGTTGATGTGCGCCGGATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCCGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATGCAGGTCGG                                                                                                            >  SRR19973061.3648709/1‑150 (MQ=60)
                                              CGAATCTTCGCAGTCGAGAATGGTACTGATAGCAGCTTCGACGATAACATCGTTGATGTGCGCCGGATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCCGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATGCAGGTCGGCGC                                                                                                         >  SRR19973061.3557308/1‑150 (MQ=60)
                                              CGAATCTTCGCAGTCGAGAATGGTACTGATAGCAGCTTCGACGATAACATCGTTGATGTGCGCCGGATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCCGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATGCAGGTCGGCGC                                                                                                         >  SRR19973061.3557375/1‑150 (MQ=60)
                                               GAATCTTCGCAGTCGAGAATGGTACTGATAGCAGCTTCGACGATAACATCGTTGATGTGCGCCGGATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCCGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATGCAGGTCGGCGCA                                                                                                        >  SRR19973061.1516770/1‑150 (MQ=60)
                                               GAATCTTCGCAGTCGAGAATGGTACTGATAGCAGCTTCGACGATAACATCGTTGATGTGCGCCGGATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCCGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATGCAGGTCGGCGCA                                                                                                        >  SRR19973061.1600024/1‑150 (MQ=60)
                                               GAATCTTCGCAGTCGAGAATGGTACTGATAGCAGCTTCGACGATAACATCGTTGATGTGCGCCGGATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCCGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATGCAGGTCGGCGCA                                                                                                        >  SRR19973061.590749/1‑150 (MQ=60)
                                                AATCTTCGCAGTCGAGAATGGTACTGATAGCAGCTTCGACGATAACATCGTTGATGTGCGCCGGATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCCGCTCAATATGCCGGCCGTTATTTTTCAGCAAAATGCAGGTCGGCGCAG                                                                                                       >  SRR19973061.253896/1‑150 (MQ=60)
                                                AATCTTCGCAGTCGAGAATGGTACTGATAGCAGCTTCGACGATAACATCGTTGATGTGCGCCGGATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCCGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATGCAGGTCGGCGCAG                                                                                                       >  SRR19973061.358954/1‑150 (MQ=60)
                                                AATCTTCGCAGTCGAGAATGGTACTGATAGCAGCTTCGACGATAACATCGTTGATGTGCGCCGGATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCCGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATGCAGGTCGGCGCAG                                                                                                       >  SRR19973061.1425623/1‑150 (MQ=60)
                                                 ATCTTCGCAGTCGAGAATGGTACTGATAGCAGCTTCGACGATAACATCGTTGATGTGCGCCGGATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCCGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATGCAGGTCGGCGCAGC                                                                                                      >  SRR19973061.1846183/1‑150 (MQ=60)
                                                 ATCTTCGCAGTCGAGAATGGTACTGATAGCAGCTTCGACGATAACATCGTTGATGTGCGCCGGATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCCGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATGCAGGTCGGCGCAGC                                                                                                      >  SRR19973061.1846742/1‑150 (MQ=60)
                                                 ATCTTCGCAGTCGAGAATGGTACTGATAGCAGCTTCGACGATAACATCGTTGATGTGCGCCGGATCGTCTTTGCCAATCCGCCAATTGGCATCGATTTGCCGCTCAATATGCAGGCCGTTATTTTTCAGAAAAATGCAGGTCGGCGCAGC                                                                                                      >  SRR19973061.2382325/1‑150 (MQ=60)
                                                      CGCAGTCGAGAATGGTACTGATAGCAGCTTCGACGATAACATCGTTGATGTGCGCCGGATCGTCTTTGCCAATCCGCCCATTGGCATCTATTTGCCGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATGCAGGTCGGCGCAGCGGCAT                                                                                                 >  SRR19973061.1503630/1‑150 (MQ=60)
                                                       GCAGTCGAGAATGGTACTGATAGCAGCTTCGACGATAACATCGTTGATGTGCGCCGGATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCCGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATGCAGGTCGGCGCAGCGGCATC                                                                                                <  SRR19973061.214814/150‑1 (MQ=60)
                                                       GCAGTCGAGAATGGTACTGATAGCAGCTTCGACGATAACATCGTTGATGTGCGCCGGATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCCGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATGCAGGTCGGCGCAGCGGCATC                                                                                                <  SRR19973061.214686/150‑1 (MQ=60)
                                                        CAGTCGAGAATGGTACTGATAGCAGCTTCGACGATAACATCGTTGATGTGCGCCGGATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCCGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATGCAGGTCGGCGCAGCGGCATCG                                                                                               <  SRR19973061.1278162/150‑1 (MQ=60)
                                                         AGTCGAGAATGGTACTGATAGCAGCTTCGACGATAACATCGTTGATGTGCGCCGGATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCCGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATGCAGGTCGGCGCAGCGGCATCGC                                                                                              >  SRR19973061.2300660/1‑150 (MQ=60)
                                                            CGAGAATGGTACTGATAGCAGCTTCGACGATAACATCGTTGATGTGCGCCGGATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCCGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATGCAGGTCGGCGCAGCGGCATCGCCAC                                                                                           <  SRR19973061.2502338/150‑1 (MQ=60)
                                                             GAGAATGGTACTGATAGCAGCTTCGACGATAACATCGTTGATGTGCGCCGGATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCCGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATGCAGGTCGGCGCAGCGGCATCGCCACG                                                                                          >  SRR19973061.3811887/1‑150 (MQ=60)
                                                               GAATGGTACTGATAGCAGCTTCGACGATAACATCGTTGATGTGCGCCGGATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCCGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATGCAGGTCGGCGCAGCGGCATCGCCACGGT                                                                                        <  SRR19973061.525749/150‑1 (MQ=60)
                                                                AATGGTACTGATAGCAGCTTCGACGATAACATCGTTGATGTGCGCCGGATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCCGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATGCAGGTCGGCGCAGCGGCATCGCCACGGTA                                                                                       >  SRR19973061.1755122/1‑150 (MQ=60)
                                                                AATGGTACTGATAGCAGCTTCGACGATAACATCGTTGATGTGCGCCGGATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCCGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATGCAGGTCGGCGCAGCGGCATCGCCACGGTA                                                                                       <  SRR19973061.238165/150‑1 (MQ=60)
                                                                  TGGTACTGATAGCAGCTTCGACGATAACATCGTTGATGTGCGCCGGATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCCGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATGCAGGTCGGCGCAGCGGCATCGCCACGGTAAC                                                                                     <  SRR19973061.697036/150‑1 (MQ=60)
                                                                    GTACTGATAGCAGCTTCGACGATAACATCGTTGATGTGCGCCGGATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCCGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATGCAGGTCGGCGCAGCGGCATCGCCACGGTAACCG                                                                                   <  SRR19973061.415189/150‑1 (MQ=60)
                                                                     TACTGATAGCAGCTTCGACGATAACATCGTTGATGTGCGCCGGATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCCGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATGCAGGTCGGCGCAGCGGCATCGCCACGGTAACCGA                                                                                  >  SRR19973061.1802771/1‑150 (MQ=60)
                                                                         GATAGCAGCTTCGACGATAACATCGTTGATGTGCGCCGGATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCCGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATGCAGGTCGGCGCAGCGGCATCGCCACGGTAACCGACAAA                                                                              <  SRR19973061.3197629/150‑1 (MQ=60)
                                                                          ATAGCAGCTTCGACGATAACATCGTTGATGTGCGCCGGATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCCGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATGCAGGTCGGCGCAGCGGCATCGCCACGGTAACCGACAAAC                                                                             >  SRR19973061.3547535/1‑150 (MQ=60)
                                                                            AGCAGCTTCGACGATAACATCGTTGATGTGCGCCGGATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCCGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATGCAGGTCGGCGCAGCGGCATCGCCACGGTAACCGACAAACTG                                                                           >  SRR19973061.3113140/1‑150 (MQ=60)
                                                                              CAGCTTCGACGATAACATCGTTGATGTGCGCCGGATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCCGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATGCAGGTCGGCGCAGCGGCATCGCCACGGTAACCGACAAACTGTG                                                                         <  SRR19973061.2586707/150‑1 (MQ=60)
                                                                                   TCGACGATAACATCGTTGATGTGCGCCGGATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCCGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATGCAGGTCGGCGCAGCGGCATCGCCACGGTAACCGACAAACTGTGCTGGA                                                                    <  SRR19973061.2812610/150‑1 (MQ=60)
                                                                                    CGACGATAACATCGTTGATGTGCGCCGGATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCCGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATGCAGGTCGGCGCAGCGGCATCGCCACGGTAACCGACAAACTGTGCTGGAG                                                                   >  SRR19973061.528602/1‑150 (MQ=60)
                                                                                     GACGATAACATCGTTGATGTGCGCCGGATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCCGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATGCAGGTCGGCGCAGCGGCATCGCCACGGTAACCGACAAACTGTGCTGGAGT                                                                  <  SRR19973061.3642216/150‑1 (MQ=60)
                                                                                     GACGATAACATCGTTGATGTGCGCCGGATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCCGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATGCAGGTCGGCGCAGCGGCATCGCCACGGTAACCGACAAACTGTGCTGGAGT                                                                  <  SRR19973061.1166947/150‑1 (MQ=60)
                                                                                       CGATAACATCGTTGATGTGCGCCGGATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCCGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATGCAGGTCGGCGCAGCGGCATCGCCACGGTAACCGACAAACTGTGCTGGAGTAC                                                                <  SRR19973061.548437/150‑1 (MQ=60)
                                                                                       CGATAACATCGTTGATGTGCGCCGGATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCCGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATGCAGGTCGGCGCAGCGGCATCGCCACGGTAACCGACAAACTGTGCTGGAGTAC                                                                <  SRR19973061.430598/150‑1 (MQ=60)
                                                                                        GATAACATCGTTGATGTGCGCCGGATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCCGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATGCAGGTCGGCGCAGCGGCATCGCCACGGTAACCGACAAACTGTGCTGGAGTACG                                                               <  SRR19973061.1087456/150‑1 (MQ=60)
                                                                                        GATAACATCGTTGATGTGCGCCGGATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCCGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATGCAGGTCGGCGCAGCGGCATCGCCACGGTAACCGACAAACTGTGCTGGAGTACG                                                               <  SRR19973061.2073198/150‑1 (MQ=60)
                                                                                                CGTTGATGTGCGCCGGATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCCGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATGCAGGTCGGCGCAGCGGCATCGCCACGGTAACCGACAAACTGTGCTGGAGTACGTAACGTGG                                                       >  SRR19973061.3376380/1‑150 (MQ=60)
                                                                                                   TGATGTGCGCCGGATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCCGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATGCAGGTCGGCGCAGCGGCATCGCCACGGTAACCGACAAACTGTGCTGGAGTACGTAACGTGGTTT                                                    >  SRR19973061.1107345/1‑150 (MQ=60)
                                                                                                   TGATGTGCGCCGGATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCCGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATGCAGGTCGACGCCTCGGCATCGCCACTGAAACCGACAAACTGTGCTGGAGTACGTAACGTGGTTT                                                    >  SRR19973061.3013578/1‑150 (MQ=60)
                                                                                                    GATGTGCGCCGGATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCCGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATGCAGGTCGGCGCAGCGGCATCGCCACGGTAACCGACAAACTGTGCTGGAGTACGTAACGTGGTTTC                                                   <  SRR19973061.1609100/150‑1 (MQ=60)
                                                                                                     ATGTGCGCCGGATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCCGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATGCAGGTCGGCGCAGCGGCATCGCCACGGTAACCGACAAACTGTGCTGGAGTACGTAACGTGGTTTCT                                                  <  SRR19973061.995592/150‑1 (MQ=60)
                                                                                                     ATGTGCGCCGGATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCCGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATGCAGGTCGGCGCAGCGGCATCGCCACGGTAACCGACAAACTGTGCTGGAGTACGTAACGTGGTTTCT                                                  <  SRR19973061.2904514/150‑1 (MQ=60)
                                                                                                     ATGTGCGCCGGATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCCGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATGCAGGTCGGCGCAGCGGCATCGCCACGGTAACCGACAAACTGTGCTGGAGTACGTAACGTGGTTTCT                                                  <  SRR19973061.2365786/150‑1 (MQ=60)
                                                                                                        TGCGCCGGATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCCGCTCAATATGCCGGCCGTTATTTTTCAGCAAAATGCAGGTCGGCGCAGCGGCATCGCCACGGTAACCGACAAACTGTGCTGGAGTACGTAACGTGGTTTCTTTA                                               >  SRR19973061.2309543/1‑150 (MQ=60)
                                                                                                        TGCGCCGGATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCCGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATGCAGGTCGGCGCAGCGGCATCGCCACGGTAACCGACAAACTGTGCTGGAGTACGTAACGTGGTTTCTTTA                                               >  SRR19973061.3791677/1‑150 (MQ=60)
                                                                                                             CGGATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCCGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATGCAGGTCGGCGCAGCGGCATCGCCACGGTAACCGACAAACTGTGCTGGAGTACGTAACGTGGTTTCTTTACCATT                                          <  SRR19973061.3043681/150‑1 (MQ=60)
                                                                                                             CGGATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCCGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATGCAGGTCGGCGCAGCGGCATCGCCACGGTAACCGACAAACTGTGCTGGAGTACGTAACGTGGTTTCTTTACCATT                                          >  SRR19973061.2898251/1‑150 (MQ=60)
                                                                                                              GGATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCCGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATGCAGGTCGGCGCAGCGGCATCGCCACGGTAACCGACAAACTGTGCTGGAGTACGTAACGTGGTTTCTTTACCATTT                                         >  SRR19973061.1705061/1‑150 (MQ=60)
                                                                                                               GATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCCGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATGCAGGTCGGCGCAGCGGCATCGCCACGGTAACCGACAAACTGTGCTGGAGTACGTAACGTGGTTTCTTTACCATTTT                                        >  SRR19973061.3262800/1‑150 (MQ=60)
                                                                                                               GATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCCGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATGCAGGTCGGCGCAGCGGCATCGCCACGGTAACCGACAAACTGTGCTGGAGTACGTAACGTGGTTTCTTTACCATTTT                                        >  SRR19973061.1357257/1‑150 (MQ=60)
                                                                                                                ATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCCGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATGCAGGTCGGCGCAGCGGCATCGCCACGGTAACCGACAAACTGTGCTGGAGTACGTAACGTGGTTTCTTTACCATTTTT                                       >  SRR19973061.3774457/1‑150 (MQ=60)
                                                                                                                 TCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCCGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATGCAGGTCGGCGCAGCGGCATCGCCACGGTAACCGACAAACTGTGCTGGAGTACGTAACGTGGTTTCTTTACCATTTTTC                                      >  SRR19973061.2065827/1‑150 (MQ=60)
                                                                                                                 TCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCCGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATGCAGGTCGGCGCAGCGGCATCGCCACGGTAACCGACAAACTGTGCTGGAGTACGTAACGTGGTTTCTTTACCATTTTTC                                      <  SRR19973061.655172/150‑1 (MQ=60)
                                                                                                                  CGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCCGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATGCAGGTCGGCGCAGCGGCATCGCCACGGTAACCGACAAACTGTGCTGGAGTACGTAACGTGGTTTCTTTACCATTTTTCA                                     >  SRR19973061.510479/1‑150 (MQ=60)
                                                                                                                   GTCTTTGCCAATCCGCCCATTGGCATCGATTTGCCGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATGCAGGTCGGCGCAGCGGCATCGCCACGGTAACCGACAAACTGTGCTGGAGTACGTAACGTGGTTTCTTTACCATTTTTCAA                                    >  SRR19973061.2666132/1‑150 (MQ=60)
                                                                                                                   GTCTTTGCCAATCCGCCCATTGGCATCGATTTGCCGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATGCAGGTCGGCGCAGCGGCATCGCCACGGTAACCGACAAACTGTGCTGGAGTACGTAACGTGGTTTCTTTACCATTTTTCAA                                    <  SRR19973061.1788790/150‑1 (MQ=60)
                                                                                                                   GTATTTGCCAATCCGCCCATTGGCATCGATTTGCCGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATGCAGGTCGGCGCAGCGGCATCGCCACGGTAACCGACAAACTGTGCTGGAGTACGTAACGTGGTTTCTTTACCATTTTTCAA                                    <  SRR19973061.1788776/150‑1 (MQ=60)
                                                                                                                       TTGCCAATCCGCCCATTGGCATCGATTTGCCGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATGCAGGTCGGCGCAGCGGCATCGCCACGGTAACCGACAAACTGTGCTGGAGTACGTAACGTGGTTTCTTTACCATTTTTCAACTGG                                <  SRR19973061.3456134/150‑1 (MQ=60)
                                                                                                                       TTGCCAATCCGCCCATTGGCATCGATTTGCCGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATGCAGGTCGGCGCAGCGGCATCGCCACGGTAACCGACAAACTGTGCTGGAGTACGTAACGTGGTTTCTTTACCATTTTTCAACTGG                                >  SRR19973061.3184641/1‑150 (MQ=60)
                                                                                                                        TGCCAATCCGCCCATTGGCATCGATTTGCCGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATGCAGGTCGGCGCAGCGGCATCGCCACGGTAACCGACAAACTGTGCTGGAGTACGTAACGTGGTTTCTTTACCATTTTTCAACTGGA                               >  SRR19973061.905180/1‑150 (MQ=60)
                                                                                                                        TGCCAATCCGCCCATTGGCATCGATTTGCCGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATGCAGGTCGGCGCAGCGGCATCGCCACGGTAACCGACAAACTGTGCTGGAGTACGTAACGTGGTTTCTTTACCATTTTTCAACTGGA                               >  SRR19973061.910204/1‑150 (MQ=60)
                                                                                                                         GCCAATCCGCCCATTGGCATCGATTTGCCGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATGCAGGTCGGCGCAGCGGCATCGCCACGGTAACCGACAAACTGTGCTGGAGTACGTAACGTGGTTTCTTTACCATTTTTCAACTGGAT                              >  SRR19973061.1954212/1‑150 (MQ=60)
                                                                                                                           CAATCCGCCCATTGGCATCGATTTGCCGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATGCAGGTCGGCGCAGCGGCATCGCCACGGTAACCGACAAACTGTGCTGGAGTACGTAACGTGGTTTCTTTACCATTTTTCAACTGGATGC                            <  SRR19973061.420258/150‑1 (MQ=60)
                                                                                                                              TCCGCCCATTGGCATCGATTTGCCGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATGCAGGTCGGCGCAGCGGCATCGCCACGGTAACCGACAAACTGTGCTGGAGTACGTAACGTGGTTTCTTTACCATTTTTCAACTGGATGCGTA                         <  SRR19973061.3584757/150‑1 (MQ=60)
                                                                                                                              TCCGCCCATTGGCATCGATTTGCCGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATGCAGGTCGGCGCAGCGGCATCGCCACGGTAACCGACAAACTGTGCTGGAGTACGTAACGTGGTTTCTTTACCATTTTTCAACTGGATGCGTA                         >  SRR19973061.3262069/1‑150 (MQ=60)
                                                                                                                                 GCCCATTGGCATCGATTTGCCGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATGCAGGTCGGCGCAGCGGCATCGCCACGGTAACCGACAAACTGTGCTGGAGTACGTAACGTGGTTTCTTTACCATTTTTCAACTGGATGCGTAATT                      <  SRR19973061.509672/150‑1 (MQ=60)
                                                                                                                                 GCCCATTGGCATCGATTTGCCGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATGCAGGTCGGCGCAGCGGCATCGCCACGGTAACCGACAAACTGTGCTGGAGTACGTAACGTGGTTTCTTTACCATTTTTCAACTGGATGCGTAATT                      <  SRR19973061.3447556/150‑1 (MQ=60)
                                                                                                                                    CATTGGCATCGATTTGCCGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATGCAGGTCGGCGCAGCGGCATCGCCACGGTAACCGACAAACTGTGCTGGAGTACGTAACGTGGTTTCTTTACCATTTTTCAACTGGATGCGTAATTGTT                   <  SRR19973061.3076122/150‑1 (MQ=60)
                                                                                                                                     ATTGGCATCGATTTGCCGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATGCAGGTCGGCGCAGCGGCATCGCCACGGTAACCGACAAACTGTGCTGGAGTACGTAACGTGGTTTCTTTACCATTTTTCAACTGGATGCGTAATTGTTT                  >  SRR19973061.3794232/1‑150 (MQ=60)
                                                                                                                                         GCATCGATTTGCCGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATGCAGGTCGGCGCAGCGGCATCGCCACGGTAACCGACAAACTGTGCTGGAGTACGTAACGTGGTTTCTTTACCATTTTTCAACTGGATGCGTAATTGTTTATCA              <  SRR19973061.3755745/150‑1 (MQ=60)
                                                                                                                                          CATCGATTTGCCGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATGCAGGTCGGCGCAGCGGCATCGCCACGGTAACCGACAAACTGTGCTGGAGTACGTAACGTGGTTTCTTTACCATTTTTCAACTGGATGCGTAATTGTTTATCAA             >  SRR19973061.546750/1‑150 (MQ=60)
                                                                                                                                           ATCGATTTGTCGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATGCAGGTCGGCGCAGCGGCATCGCCACGGTAACCGACAAACTGTGCTGGAGTACGTAACGTGGTTTCTTTACCATTTTTCAACTGGATGCGTAATTGTTTATCAAC            >  SRR19973061.415931/1‑150 (MQ=60)
                                                                                                                                            TCGATTTGCCGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATGCAGGTCGGCGCAGCGGCATCGCCACGGTAACCGACAAACTGTGCTGGAGTACGTAACGTGGTTTCTTTACCATTTTTCAACTGGATGCGTAATTGTTTATCAACC           <  SRR19973061.2579822/150‑1 (MQ=60)
                                                                                                                                                 TTGCCGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATGCAGGTCGGCGCAGCGGCATCGCCACGGTAACCGACAAACTGTGCTGGAGTACGTAACGTGGTTTCTTTACCATTTTTCAACTGGATGCGTAATTGTTTATCAACCACCTT      <  SRR19973061.1629893/150‑1 (MQ=60)
                                                                                                                                                 TTGCCGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATGCAGGTCGGCGCAGCGGCATCGCCACGGTAACCGACAAACTGTGCTGGAGTACGTAACGTGGTTTCTTTACCATTTTTCAACTGGATGCGTAATTGTTTATCAACCACCTT      <  SRR19973061.143885/150‑1 (MQ=60)
                                                                                                                                                 TTGCCGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATGCAGGTCGGCGCAGCGGCATCGCCACGGTAACCGACAAACTGTGCTGGAGTACGTAACGTGGTTTCTTTACCATTTTTCAACTGGATGCGTAATTGTTTATCAACCACCTT      <  SRR19973061.143810/150‑1 (MQ=60)
                                                                                                                                                  TGCCGCTCAATATGCAGGCTGTTATTTTTCAGCAAAATGTAGGTCGGCGCAGCGGCATCGCCATGGTAACCGAAAAAATTTGCTGGAGTACGTAACGTGGTTTCTTTACCATTTTTCTACTGGATGCGTAATTGTTTATCAACCACCTTA     <  SRR19973061.2291430/150‑1 (MQ=60)
                                                                                                                                                  TGCCGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATGCAGGTCGGCGCAGCGGCATCGCCACGGTAACCGACAAACTGTGCTGGAGTACGTAACGTGGTTTCTTTACCATTTTTCAACTGGATGCGTAATTGTTTATCAACCACCTTA     >  SRR19973061.110971/1‑150 (MQ=60)
                                                                                                                                                    CCGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATGCAGGTCGGCGCAGCGGCATCGCCACGGTAACCGACAAACTGTGCTGGAGTACGTAACGTGGTTTCTTTACCATTTTTCAACTGGATGCGTAATTGTTTATCAACCACCTTAAA   <  SRR19973061.1313635/150‑1 (MQ=60)
                                                                                                                                                     CGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATGCAGGTCGGCGCAGCGGCATCGCCACGGTAACCGACAAACTGTGCTGGAGTACGTAACGTGGTTTCTTTACCATTTTTCAACTGGATGCGTAATTGTTTATCAACCACCTTAAAC  >  SRR19973061.2072726/1‑150 (MQ=60)
                                                                                                                                                     CGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATGCAGGTCGGCGCAGCGGCATCGCCACGGTAACCGACAAACTGTGCTGGAGTACGTAACGTGGTTTCTTTACCATTTTTCAACTGGATGCGTAATTGTTTATCAACCACCTTAAAC  <  SRR19973061.2036919/150‑1 (MQ=60)
                                                                                                                                                     |                                                                                                                                                     
GGTTGCGGTACAGCAGGATTTTATCTTCCGCATCAACCGCCGCGACCGAATCTTCGCAGTCGAGAATGGTACTGATAGCAGCTTCGACGATAACATCGTTGATGTGCGCCGGATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCAGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATGCAGGTCGGCGCAGCGGCATCGCCACGGTAACCGACAAACTGTGCTGGAGTACGTAACGTGGTTTCTTTACCATTTTTCAACTGGATGCGTAATTGTTTATCAACCACCTTAAAC  >  CP009273/3116297‑3116595

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑