Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F488 I0 R1 1275 300.1 10328444 91.0% 9398884 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,820,888 C→A 100% C98F (TGC→TTC)  astE ← succinylglutamate desuccinylase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,820,8880CA99.2% 911.6 / ‑2.8 274C98F (TGC→TTC) astEsuccinylglutamate desuccinylase
Reads supporting (aligned to +/- strand):  ref base C (1/2);  new base A (140/131);  total (141/133)
Fisher's exact test for biased strand distribution p-value = 6.13e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 277 total aligned reads displayed.

TGTAGATCAAGGTGCCAGCGCACAGATTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGTTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGCAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTGAGATATCGCGCCAAGCAACGCGTCCAGCATCTCCACAGGTGCCGTCTCATTACCGTGTATTCCCGCT  >  CP009273/1820741‑1821034
                                                                                                                                                   |                                                                                                                                                  
tGTAGATCAAGGTGCCAGCGCACAGATTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGTTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGaaa                                                                                                                                                  >  1:1914512/1‑150 (MQ=255)
tGTAGATCAAGGTGCCAGCGCACAGATTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGTTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGaaa                                                                                                                                                  >  1:4099446/1‑150 (MQ=255)
 gTAGATCAAGGTGCCAGCGCACAGATTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGTTCCAGTTCGCGCGCCCGACAGTTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAAt                                                                                                                                                 <  2:2530199/150‑1 (MQ=255)
 gTAGATCAAGGTGCCAGCGCACAGATTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGTTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAAt                                                                                                                                                 <  2:431710/150‑1 (MQ=255)
 gTAGATCAAGGTGCCAGCGCACAGATTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGTTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAAt                                                                                                                                                 >  1:3137639/1‑150 (MQ=255)
   aGATCAAGGTGCCAGCGCACAGATTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGTTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATaa                                                                                                                                               >  1:2738298/1‑150 (MQ=255)
    gATCAAGGTGCCAGCGCACGGATTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGTTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAAc                                                                                                                                              <  2:3994826/150‑1 (MQ=255)
     aTCAAGGTGCCAGCGCACAGATTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGTTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACg                                                                                                                                             <  2:2871664/150‑1 (MQ=255)
     aTCAAGGTGCCAGCGCACAGATTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGTTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACg                                                                                                                                             >  1:1620440/1‑150 (MQ=255)
     aTCAAGGTGCCAGCGCACAGATTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGTTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACg                                                                                                                                             >  1:3654551/1‑150 (MQ=255)
     aTCAAGGTGCCAGCGCACAGATTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGTTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACg                                                                                                                                             <  2:4739833/150‑1 (MQ=255)
        aaGGTGCCAGCGCACAGATTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGTTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGttt                                                                                                                                          >  1:5067429/1‑150 (MQ=255)
          ggTGCCAGCGCACAGATTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGTTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTcc                                                                                                                                        <  1:2651317/150‑1 (MQ=255)
           gTGCCAGCGCACAGATTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGTTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTccc                                                                                                                                       >  2:4433148/1‑150 (MQ=255)
           gTGCCAGCGCACAGATTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGTTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTccc                                                                                                                                       <  2:4342533/150‑1 (MQ=255)
            tGCCAGCGCACAGATTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGTTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCt                                                                                                                                      <  2:4808267/150‑1 (MQ=255)
            tGCCAGCGCACAGATTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGTTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCt                                                                                                                                      <  2:1708854/150‑1 (MQ=255)
              ccAGCGCACAGATTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGTTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTg                                                                                                                                    <  2:218297/150‑1 (MQ=255)
                aGCGCACAGATTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGTTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCt                                                                                                                                  <  1:2226401/150‑1 (MQ=255)
                aGCGCACAGATTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGTTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCt                                                                                                                                  <  1:787313/150‑1 (MQ=255)
                aGCGCACAGATTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGTTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCt                                                                                                                                  >  1:1408400/1‑150 (MQ=255)
                aGCGCACAGATTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGTTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCt                                                                                                                                  >  1:1367390/1‑150 (MQ=255)
                aGCGCAATGATTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGTTCCTGTTCGCGCGCCCTTAAGGTTTCTCCGGTTTCTGCAATTTGCTGCCAACGACCGCCTTTCATTCGTTTCATTTCGCTTTGGAAATAACGTTTCCCTTGCt                                                                                                                                  <  1:2093576/150‑1 (MQ=255)
                 gcgcACAGATTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGTTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCtt                                                                                                                                 <  2:4958694/150‑1 (MQ=255)
                   gcACAGATTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGTTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCa                                                                                                                               <  2:2772206/150‑1 (MQ=255)
                    cacaGATTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGTTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAg                                                                                                                              <  1:1551822/150‑1 (MQ=255)
                     acaGATTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGTTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAgc                                                                                                                             >  1:510780/1‑150 (MQ=255)
                        gATTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGTTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCa                                                                                                                          >  2:131248/1‑150 (MQ=255)
                        gATTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGTTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCa                                                                                                                          <  2:3285782/150‑1 (MQ=255)
                          ttCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGTTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCagg                                                                                                                        <  2:1229027/150‑1 (MQ=255)
                                 cccTGGTCATAAAAATCTTCCAGGCACTGTTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTc                                                                                                                 >  1:1170015/1‑150 (MQ=255)
                                 cccTGGTCATAAAAATCTTCCAGGCACTGTTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTc                                                                                                                 >  1:243182/1‑150 (MQ=255)
                                  ccTGGTCATAACAATCTTCCAGGCAATGTTCCAGTTCGCGCGCCCGACTGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTAATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTcc                                                                                                                <  1:3577826/150‑1 (MQ=255)
                                     ggTCATAAAAATCTTCCAGGCACTGTTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGa                                                                                                             <  1:2853611/150‑1 (MQ=255)
                                     ggTCATAAAAATCTTCCAGGCACTGTTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGa                                                                                                             <  2:4830562/150‑1 (MQ=255)
                                      gTCATAAAAATCTTCCAGGCACTGTTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAg                                                                                                            >  1:2881452/1‑150 (MQ=255)
                                       tCATAAAAATCTTCCAGGCACTGTTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAgg                                                                                                           <  1:97299/150‑1 (MQ=255)
                                              aaTCTTCCAGGCACTGTTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCa                                                                                                    >  2:2334673/1‑150 (MQ=255)
                                              aaTCTTCCAGGCACTGTTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCa                                                                                                    >  2:4894294/1‑150 (MQ=255)
                                              aaTCTTCCAGGCACTGTTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCa                                                                                                    <  2:5141132/150‑1 (MQ=255)
                                               aTCTTCCAGGCACTGTTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAg                                                                                                   <  1:3254065/150‑1 (MQ=255)
                                                  ttCCAGGCACTGTTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCaa                                                                                                >  2:1952644/1‑150 (MQ=255)
                                                      aGGCACTGTTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGc                                                                                            >  2:1407748/1‑150 (MQ=255)
                                                      aGGCACTGTTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGc                                                                                            >  1:4933203/1‑150 (MQ=255)
                                                       ggCACTGTTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGcc                                                                                           >  2:1509919/1‑150 (MQ=255)
                                                         cACTGTTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCaa                                                                                         >  2:2603361/1‑150 (MQ=255)
                                                         cACTGTTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCaa                                                                                         <  1:3324385/150‑1 (MQ=255)
                                                         cACTGTTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCaa                                                                                         >  2:1893301/1‑150 (MQ=255)
                                                         cACTGTTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCaa                                                                                         <  1:1842448/150‑1 (MQ=255)
                                                             gTTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTa                                                                                     <  2:1553151/150‑1 (MQ=255)
                                                                  aGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACggg                                                                                >  2:3719364/1‑150 (MQ=255)
                                                                      cgcgcgCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGAtct                                                                            <  2:1863789/150‑1 (MQ=255)
                                                                      cgcgcgCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGAtct                                                                            >  2:4297866/1‑150 (MQ=255)
                                                                       gcgcgcCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGAtctc                                                                           <  1:1757575/150‑1 (MQ=255)
                                                                        cgcgcCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCg                                                                          >  2:40541/1‑150 (MQ=255)
                                                                        cgcgcCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCg                                                                          >  2:1160771/1‑150 (MQ=255)
                                                                          cgcCCTAAAGGTTTCTCCTCTTACAGCAATTAGCTGCCAACGACCTCCAAACATTCGATTCATATCGCTATTGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGcc                                                                        <  1:2580309/150‑1 (MQ=255)
                                                                          cgcCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGcc                                                                        >  1:3493069/1‑150 (MQ=255)
                                                                            cccGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGt                                                                      >  2:3075502/1‑150 (MQ=255)
                                                                              cGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCAc                                                                                                      >  1:4330575/1‑116 (MQ=255)
                                                                               gACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTgag                                                                   >  2:1783195/1‑150 (MQ=255)
                                                                               gACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTg                                                                     <  2:815058/148‑1 (MQ=255)
                                                                                aCAGGTTTCTCCGCTTTCAGCAAAAAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTgaga                                                                  >  2:2394092/1‑150 (MQ=255)
                                                                                  aGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTGAGata                                                                <  2:629458/150‑1 (MQ=255)
                                                                                        ctcCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTGAGATATCgcgc                                                          >  2:2635221/1‑150 (MQ=255)
                                                                                          ccGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTGAGATATCGCGCCa                                                        <  1:4883910/150‑1 (MQ=255)
                                                                                          ccGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTGAGATATCGCGCCa                                                        <  1:4248870/150‑1 (MQ=255)
                                                                                            gCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTGAGATATCGCGCCAAg                                                      <  1:767358/150‑1 (MQ=255)
                                                                                              tttCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTGAGATATCGCGCCAAGCa                                                    <  1:122034/150‑1 (MQ=255)
                                                                                               ttCAGCAAATAGCTGCCACCGACCGCCAAACATTCGATTCATATCGCTATGGAAATCACGGGTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTGAGATATCGCGCCAAGCaa                                                   <  1:2024095/150‑1 (MQ=255)
                                                                                               ttCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTGAGATATCGCGCCAAGCaa                                                   >  2:2026382/1‑150 (MQ=255)
                                                                                                 cAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTGAGATATCGCGCCAAGCAAcg                                                 <  1:3450530/150‑1 (MQ=255)
                                                                                                 cAGCAAATAGCTACCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTGAGATATCGCGCCAAGCAAcg                                                 <  1:2091358/150‑1 (MQ=255)
                                                                                                      aaTAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTGAGATATCGCGCCAAGCAACGCGTcc                                            >  1:920343/1‑150 (MQ=255)
                                                                                                      aaTAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTGAGATATCGCGCCAAGCAACGCGTcc                                            <  1:3975592/150‑1 (MQ=255)
                                                                                                       atatCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTGAGATATCGCGCCAAGCAACGCGTCCa                                           >  2:1933435/1‑150 (MQ=255)
                                                                                                       aTAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTGAGATATCGCGCCAAGCAACGCGTCCa                                           >  1:2577129/1‑150 (MQ=255)
                                                                                                        tAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTGAGATATCGCGCCAAGCAACGCGTCCAg                                          <  2:1851010/150‑1 (MQ=255)
                                                                                                         tgCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTGAGATATCGCGCCAAGCAACGCGTCCAGc                                         <  2:3016838/149‑1 (MQ=255)
                                                                                                            tGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTGAGATATCGCGCCAAGCAACGCGTCCAGCAtc                                      >  1:1731408/1‑150 (MQ=255)
                                                                                                             gCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTGAGATATCGCGCCAAGCAACGCGTCCAGCAtct                                     <  1:2974302/150‑1 (MQ=255)
                                                                                                              ccAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTGAGATATCGCGCCAAGCAACGCGTCCAGCAtctc                                    >  1:4546059/1‑150 (MQ=255)
                                                                                                                  cGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTGAGATATCGCGCCAAGCAACGCGTCCAGCATCTCcaca                                >  2:3486751/1‑150 (MQ=255)
                                                                                                                  cGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTGAGATATCGCGCCAAGCAACGCGTCCAGCATCTCcaca                                >  2:2803008/1‑150 (MQ=255)
                                                                                                                   gACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTGAGATATCGCGCCAAGCAACGCGTCCAGCATCTCCACAg                               >  1:3395743/1‑150 (MQ=255)
                                                                                                                   gACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTGAGATATCGCGCCAAGCAACGCGTCCAGCATCTCCACAg                               <  2:5040348/150‑1 (MQ=255)
                                                                                                                    aCCGCCAAACATTCGATTCATATCGCTATGGCAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTGAGATATCGCGCCAAGCAACGCGTCCAGCATCTCCACAgg                              >  2:3566353/1‑150 (MQ=255)
                                                                                                                    aCCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTGAGATATCGCGCCAAGCAACGCGTCCAGCATCTCCACAgg                              >  1:1484445/1‑150 (MQ=255)
                                                                                                                    aCCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTGAGATATCGCGCCAAGCAACGCGTCCAGCATCTCCACAgg                              <  2:563122/150‑1 (MQ=255)
                                                                                                                        ccAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTGAGATATCGCGCCAAGCAACGCGTCCAGCATCTCCACAGGTGcc                          >  1:10082/1‑150 (MQ=255)
                                                                                                                        ccAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTGAGATATCGCGCCAAGCAACGCGTCCAGCATCTCCACAGGTGcc                          >  2:893624/1‑150 (MQ=255)
                                                                                                                         cAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTGAGATATCGCGCCAAGCAACGCGTCCAGCATCTCCACAGGTGCCg                         >  2:4709709/1‑150 (MQ=255)
                                                                                                                              aTTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTGAGATATCGCGCCAAGCAACGCGTCCAGCATCTCCACAGGTGCCGTCTCa                    >  2:4618525/1‑150 (MQ=255)
                                                                                                                                  gATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTGAGATCTCGCGCCAAGCAACGCGTCCAGCATCTCCACAGGTGCCGTCTCATTAc                >  1:4759446/1‑150 (MQ=255)
                                                                                                                                  gATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTGAGATATCGCGCCAAGCAACGCGTCCAGCATCTCCACAGGTGCCGTCTCATTAc                >  2:3902434/1‑150 (MQ=255)
                                                                                                                                       atatCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTGAGATATCGCGCCAAGCAACGCGTCCAGCATCTCCACAGGTGCCGTCTCATTACCgtgt           >  2:2452349/1‑150 (MQ=255)
                                                                                                                                       atatCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTGAGATATCGCGCCAAGCAACGCGTCCAGCATCTCCACAGGTGCCGTCTCATTACCgtgt           >  1:3740906/1‑150 (MQ=255)
                                                                                                                                            gCTATGGAAATAACGTGTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTGAGATATCGCGCCAAGCAACGCGTCCAGCATCTCCACAGGTGCCGTCTCATTACCGTGTATTcc      <  2:4223300/150‑1 (MQ=255)
                                                                                                                                              tATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTGAGATATCGCGCCAAGCAACGCGTCCAGCATCTCCACAGGTGCCGTCTCATTACCGTGTATTCCCg    <  2:427886/150‑1 (MQ=255)
                                                                                                                                              tATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTGAGATATCGCGCCAAGCAACGCGTCCAGCATCTCCACAGGTGCCGTCTCATTACCGTGTATTCCCg    <  2:1630446/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
TGTAGATCAAGGTGCCAGCGCACAGATTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGTTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGCAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTGAGATATCGCGCCAAGCAACGCGTCCAGCATCTCCACAGGTGCCGTCTCATTACCGTGTATTCCCGCT  >  CP009273/1820741‑1821034

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TGTAGATCAAGGTGCCAGCGCACAGATTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGTTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGCAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTGAGATATCGCGCCAAGCAACGCGTCCAGCATCTCCACAGGTGCCGTCTCATTACCGTGTATTCCCGCTGAA  >  CP009273/1820741‑1821037
                                                                                                                                                   |                                                                                                                                                     
TGTAGATCAAGGTGCCAGCGCACAGATTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGTTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAA                                                                                                                                                     >  SRR19973063.4112582/1‑150 (MQ=60)
TGTAGATCAAGGTGCCAGCGCACAGATTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGTTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAA                                                                                                                                                     >  SRR19973063.1920501/1‑150 (MQ=60)
TGTAGATCAAGGTGCCAGCGCACAGATTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGTTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAA                                                                                                                                                     <  SRR19973063.1962545/150‑1 (MQ=60)
 GTAGATCAAGGTGCCAGCGCACAGATTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGTTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAAT                                                                                                                                                    >  SRR19973063.3147729/1‑150 (MQ=60)
   AGATCAAGGTGCCAGCGCACAGATTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGTTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAA                                                                                                                                                  >  SRR19973063.2747138/1‑150 (MQ=60)
     ATCAAGGTGCCAGCGCACAGATTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGTTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACG                                                                                                                                                >  SRR19973063.1625391/1‑150 (MQ=60)
     ATCAAGGTGCCAGCGCACAGATTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGTTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACG                                                                                                                                                >  SRR19973063.3666270/1‑150 (MQ=60)
       CAAGGTGCCAGCGCACAGATTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGTTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTT                                                                                                                                              >  SRR19973063.4236762/1‑150 (MQ=60)
       CAAGGTGCCAGCGCACAGATTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGTTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTT                                                                                                                                              <  SRR19973063.2316005/150‑1 (MQ=60)
        AAGGTGCCAGCGCACAGATTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGTTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTT                                                                                                                                             >  SRR19973063.3567400/1‑150 (MQ=60)
        AAGGTGCCAGCGCACAGATTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGTTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTT                                                                                                                                             >  SRR19973063.5083735/1‑150 (MQ=60)
         AGGTGCCAGCGCACAGATTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGTTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTC                                                                                                                                            >  SRR19973063.3414303/1‑150 (MQ=60)
          GGTGCCAGCGCACAGATTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGTTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCC                                                                                                                                           >  SRR19973063.938137/1‑150 (MQ=60)
          GGTGCCAGCGCACAGATTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGTTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCC                                                                                                                                           <  SRR19973063.2659795/150‑1 (MQ=60)
              CCAGCGCACAGATTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGTTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTG                                                                                                                                       <  SRR19973063.2083813/150‑1 (MQ=60)
              CCAGCGCACAGATTCTGTGCCCTGGTCATAAAAATCTTCCAGGCACTGGTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTGCCCTTG                                                                                                                                       <  SRR19973063.4436517/150‑1 (MQ=60)
               CAGCGCACAGATTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGTTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGC                                                                                                                                      <  SRR19973063.3731241/150‑1 (MQ=60)
               CAGCGCACAGATTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGTTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGC                                                                                                                                      <  SRR19973063.3113129/150‑1 (MQ=60)
                AGCGCACAGATTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGTTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCT                                                                                                                                     <  SRR19973063.277626/150‑1 (MQ=60)
                AGCGCACAGATTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGTTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCT                                                                                                                                     >  SRR19973063.1500440/1‑150 (MQ=60)
                AGCGCACAGATTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGTTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCT                                                                                                                                     <  SRR19973063.2233453/150‑1 (MQ=60)
                AGCGCACAGATTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGTTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCT                                                                                                                                     <  SRR19973063.2233452/150‑1 (MQ=60)
                AGCGCACAGATTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGTTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCT                                                                                                                                     <  SRR19973063.789591/150‑1 (MQ=60)
                AGCGCACAGATTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGTTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCT                                                                                                                                     <  SRR19973063.789527/150‑1 (MQ=60)
                AGCGCACAGATTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGTTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCT                                                                                                                                     <  SRR19973063.669959/150‑1 (MQ=60)
                AGCGCACAGATTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGTTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCT                                                                                                                                     <  SRR19973063.4968100/150‑1 (MQ=60)
                AGCGCACAGATTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGTTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCT                                                                                                                                     >  SRR19973063.1412666/1‑150 (MQ=60)
                AGCGCACAGATTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGTTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCT                                                                                                                                     >  SRR19973063.1371510/1‑150 (MQ=60)
                AGCGCAATGATTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGTTCCTGTTCGCGCGCCCTTAAGGTTTCTCCGGTTTCTGCAATTTGCTGCCAACGACCGCCTTTCATTCGTTTCATTTCGCTTTGGAAATAACGTTTCCCTTGCT                                                                                                                                     <  SRR19973063.2100117/150‑1 (MQ=60)
                    CACAGATTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGTTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAG                                                                                                                                 <  SRR19973063.1556577/150‑1 (MQ=60)
                    CACAGATTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGTTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAG                                                                                                                                 >  SRR19973063.1856790/1‑150 (MQ=60)
                    CACAGATTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGTTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAG                                                                                                                                 >  SRR19973063.4328589/1‑150 (MQ=60)
                     ACAGATTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGTTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGC                                                                                                                                >  SRR19973063.512267/1‑150 (MQ=60)
                     ACAGATTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGTTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGC                                                                                                                                >  SRR19973063.1173981/1‑150 (MQ=60)
                     ACAGATTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGTTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGC                                                                                                                                >  SRR19973063.3679496/1‑150 (MQ=60)
                       AGATTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGTTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGC                                                                                                                              >  SRR19973063.362365/1‑150 (MQ=60)
                       AGATTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGTTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGC                                                                                                                              >  SRR19973063.2964141/1‑150 (MQ=60)
                         ATTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGTTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAG                                                                                                                            <  SRR19973063.4702738/150‑1 (MQ=60)
                          TTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGTTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGG                                                                                                                           >  SRR19973063.4032431/1‑150 (MQ=60)
                           TCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGTTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGA                                                                                                                          <  SRR19973063.1829428/150‑1 (MQ=60)
                                 CCCTGGTCATAAAAATCTTCCAGGCACTGTTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTC                                                                                                                    >  SRR19973063.243919/1‑150 (MQ=60)
                                 CCCTGGTCATAAAAATCTTCCAGGCACTGTTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTC                                                                                                                    >  SRR19973063.1173541/1‑150 (MQ=60)
                                  CCTGGTCATAACAATCTTCCAGGCAATGTTCCAGTTCGCGCGCCCGACTGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTAATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCC                                                                                                                   <  SRR19973063.3589299/150‑1 (MQ=60)
                                  CCTGGTCATAAAAATCTTCCAGGCACTGTTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCC                                                                                                                   <  SRR19973063.940068/150‑1 (MQ=60)
                                   CTGGTCATAAAAATCTTCCAGGCACTGTTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCC                                                                                                                  <  SRR19973063.1687098/150‑1 (MQ=60)
                                   CTGGTCATAAAAATCTTCCAGGCACTGTTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCC                                                                                                                  <  SRR19973063.1687095/150‑1 (MQ=60)
                                     GGTCATAAAAATCTTCCAGGCACTGTTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGA                                                                                                                <  SRR19973063.2862757/150‑1 (MQ=60)
                                      GTCATAAAAATCTTCCAGGCACTGTTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAG                                                                                                               >  SRR19973063.2890666/1‑150 (MQ=60)
                                       TCATAAAAATCTTCCAGGCACTGTTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGG                                                                                                              <  SRR19973063.97631/150‑1 (MQ=60)
                                        CATAAAAATCTTCCAGGCACTGTTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGA                                                                                                             >  SRR19973063.4672595/1‑150 (MQ=60)
                                               ATCTTCCAGGCACTGTTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAG                                                                                                      <  SRR19973063.3264504/150‑1 (MQ=60)
                                                 CTTCCAGGCACTGTTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCA                                                                                                    >  SRR19973063.1198097/1‑150 (MQ=60)
                                                 ATTCCAGGCACTGTTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCA                                                                                                    >  SRR19973063.2635595/1‑150 (MQ=60)
                                                     CAGGCACTGTTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCG                                                                                                >  SRR19973063.1149778/1‑150 (MQ=60)
                                                     CAGGCACTGTTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCG                                                                                                >  SRR19973063.1821777/1‑150 (MQ=60)
                                                      AGGCACTGTTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGC                                                                                               >  SRR19973063.2744136/1‑150 (MQ=60)
                                                      AGGCACTGTTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGC                                                                                               >  SRR19973063.4949139/1‑150 (MQ=60)
                                                         CACTGTTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAA                                                                                            <  SRR19973063.1848202/150‑1 (MQ=60)
                                                         CACTGTTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAA                                                                                            <  SRR19973063.3334997/150‑1 (MQ=60)
                                                         CACTGTTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAA                                                                                            <  SRR19973063.3335038/150‑1 (MQ=60)
                                                             GTTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTA                                                                                        >  SRR19973063.2695764/1‑150 (MQ=60)
                                                               TCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAAC                                                                                      <  SRR19973063.392532/150‑1 (MQ=60)
                                                                 CAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAATTAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGG                                                                                    <  SRR19973063.2425359/150‑1 (MQ=60)
                                                                   GTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGA                                                                                  <  SRR19973063.1390929/150‑1 (MQ=60)
                                                                   GTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGA                                                                                  <  SRR19973063.3059034/150‑1 (MQ=60)
                                                                       GCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTC                                                                              <  SRR19973063.1762977/150‑1 (MQ=60)
                                                                          CGCCCTAAAGGTTTCTCCTCTTACAGCAATTAGCTGCCAACGACCTCCAAACATTCGATTCATATCGCTATTGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCC                                                                           <  SRR19973063.2588514/150‑1 (MQ=60)
                                                                          CGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCC                                                                           >  SRR19973063.3504237/1‑150 (MQ=60)
                                                                          CGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCC                                                                           >  SRR19973063.3483670/1‑150 (MQ=60)
                                                                          CGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCC                                                                           >  SRR19973063.3483645/1‑150 (MQ=60)
                                                                          CGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCC                                                                           <  SRR19973063.2588377/150‑1 (MQ=60)
                                                                            CCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGT                                                                         >  SRR19973063.1549899/1‑150 (MQ=60)
                                                                              CGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACagatcggaagagcacacgtctgaactccagtcac                                                                       >  SRR19973063.4344373/1‑116 (MQ=60)
                                                                               GACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTGAG                                                                      >  SRR19973063.817412/1‑150 (MQ=60)
                                                                               GACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTGAG                                                                      >  SRR19973063.817444/1‑150 (MQ=60)
                                                                                  AGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTGAGATA                                                                   >  SRR19973063.3888837/1‑150 (MQ=60)
                                                                                  AGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTGAGATA                                                                   <  SRR19973063.2909010/150‑1 (MQ=60)
                                                                                          CCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTGAGATATCGCGCCA                                                           <  SRR19973063.4262409/150‑1 (MQ=60)
                                                                                          CCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTGAGATATCGCGCCA                                                           <  SRR19973063.4899662/150‑1 (MQ=60)
                                                                                           CGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTGAGATATCGCGCCAA                                                          <  SRR19973063.4052813/150‑1 (MQ=60)
                                                                                            GCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTGAGATATCGCGCCAAG                                                         <  SRR19973063.769595/150‑1 (MQ=60)
                                                                                              TTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTGAGATATCGCGCCAAGCA                                                       <  SRR19973063.122435/150‑1 (MQ=60)
                                                                                              TTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTGAGATATCGCGCCAAGCA                                                       <  SRR19973063.4933996/150‑1 (MQ=60)
                                                                                               TTCAGCAAATAGCTGCCACCGACCGCCAAACATTCGATTCATATCGCTATGGAAATCACGGGTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTGAGATATCGCGCCAAGCAA                                                      <  SRR19973063.2030414/150‑1 (MQ=60)
                                                                                                TCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTGAGATATCGCGCCAAGCAAC                                                     <  SRR19973063.2793027/150‑1 (MQ=60)
                                                                                                TCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTGAGATATCGCGCCAAGCAAC                                                     <  SRR19973063.1285394/150‑1 (MQ=60)
                                                                                                TCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTGAGATATCGCGCCAAGCAAC                                                     <  SRR19973063.1405328/150‑1 (MQ=60)
                                                                                                 CAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTGAGATATCGCGCCAAGCAACG                                                    <  SRR19973063.3461502/150‑1 (MQ=60)
                                                                                                 CAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTGAGATATCGCGCCAAGCAACG                                                    <  SRR19973063.5110923/150‑1 (MQ=60)
                                                                                                 CAGCAAATAGCTACCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTGAGATATCGCGCCAAGCAACG                                                    <  SRR19973063.2098082/150‑1 (MQ=60)
                                                                                                 CAGCAAATAGCTACCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTGAGATATCGCGCCAAGCAACG                                                    <  SRR19973063.2097890/150‑1 (MQ=60)
                                                                                                      AATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTGAGATATCGCGCCAAGCAACGCGTCC                                               <  SRR19973063.3988327/150‑1 (MQ=60)
                                                                                                      AATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTGAGATATCGCGCCAAGCAACGCGTCC                                               >  SRR19973063.923061/1‑150 (MQ=60)
                                                                                                      AATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTGAGATATCGCGCCAAGCAACGCGTCC                                               >  SRR19973063.355805/1‑150 (MQ=60)
                                                                                                       ATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTGAGATATCGCGCCAAGCAACGCGTCCA                                              >  SRR19973063.2585329/1‑150 (MQ=60)
                                                                                                         AGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTGAGATATCGCGCCAAGCAACGCGTCCAGC                                            >  SRR19973063.2598480/1‑150 (MQ=60)
                                                                                                          GCTGCCAACGACCGCCAAACATTCTATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTGAGATATCGCGCCAAGCAACGCGTCCAGCA                                           <  SRR19973063.4321377/150‑1 (MQ=60)
                                                                                                            TGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTGAGATATCGCGCCAAGCAACGCGTCCAGCATC                                         >  SRR19973063.1736685/1‑150 (MQ=60)
                                                                                                             GCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTGAGATATCGCGCCAAGCAACGCGTCCAGCATCT                                        <  SRR19973063.2983809/150‑1 (MQ=60)
                                                                                                             GCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTGAGATATCGCGCCAAGCAACGCGTCCAGCATCT                                        <  SRR19973063.4416232/150‑1 (MQ=60)
                                                                                                              CCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTGAGATATCGCGCCAAGCAACGCGTCCAGCATCTC                                       >  SRR19973063.4560628/1‑150 (MQ=60)
                                                                                                              CCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTGAGATATCGCGCCAAGCAACGCGTCCAGCATCTC                                       <  SRR19973063.666117/150‑1 (MQ=60)
                                                                                                               CAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTGAGATATCGCGCCAAGCAACGCGTCCAGCATCTCC                                      >  SRR19973063.2572823/1‑150 (MQ=60)
                                                                                                               CAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTGAGATATCGCGCCAAGCAACGCGTCCAGCATCTCC                                      >  SRR19973063.1870568/1‑150 (MQ=60)
                                                                                                               CAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTGAGATATCGCGCCAAGCAACGCGTCCAGCATCTCC                                      >  SRR19973063.2572774/1‑150 (MQ=60)
                                                                                                               CAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTGAGATATCGCGCCAAGCAACGCGTCCAGCATCTCC                                      >  SRR19973063.5120395/1‑150 (MQ=60)
                                                                                                                  CGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTGAGATATCGCGCCAAGCAACGCGTCCAGCATCTCCACA                                   <  SRR19973063.4868030/150‑1 (MQ=60)
                                                                                                                  CGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTGAGATATCGCGCCAAGCAACGCGTCCAGCATCTCCACA                                   <  SRR19973063.2885242/150‑1 (MQ=60)
                                                                                                                   GACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTGAGATATCGCGCCAAGCAACGCGTCCAGCATCTCCACAG                                  >  SRR19973063.3406651/1‑150 (MQ=60)
                                                                                                                   GACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTGAGATATCGCGCCAAGCAACGCGTCCAGCATCTCCACAG                                  >  SRR19973063.3406550/1‑150 (MQ=60)
                                                                                                                   GACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTGAGATATCGCGCCAAGCAACGCGTCCAGCATCTCCACAG                                  >  SRR19973063.4835781/1‑150 (MQ=60)
                                                                                                                    ACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTGAGATATCGCGCCAAGCAACGCGTCCAGCATCTCCACAGG                                 >  SRR19973063.1488988/1‑150 (MQ=60)
                                                                                                                    ACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTGAGATATCGCGCCAAGCAACGCGTCCAGCATCTCCACAGG                                 >  SRR19973063.1746472/1‑150 (MQ=60)
                                                                                                                        CCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTGAGATATCGCGCCAAGCAACGCGTCCAGCATCTCCACAGGTGCC                             >  SRR19973063.10124/1‑150 (MQ=60)
                                                                                                                         CAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTGAGATATCGCGCCAAGCAACGCGTCCAGCATCTCCACAGGTGCCG                            >  SRR19973063.3204743/1‑150 (MQ=60)
                                                                                                                         CAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTGAGATATCGCGCCAAGCAACGCGTCCAGCATCTCCACAGGTGCCG                            >  SRR19973063.3204728/1‑150 (MQ=60)
                                                                                                                          AAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTGAGATATCGCGCCAAGCAACGCGTCCAGCATCTCCACAGGTGCCGT                           >  SRR19973063.1319413/1‑150 (MQ=60)
                                                                                                                                TCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTGAGATATCGCGCCAAGCAACGCGTCCAGCATCTCCACAGGTGCCGTCTCATT                     <  SRR19973063.160155/150‑1 (MQ=60)
                                                                                                                                TCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTGAGATATCGCGCCAAGCAACGCGTCCAGCATCTCCACAGGTGCCGTCTCATT                     <  SRR19973063.160105/150‑1 (MQ=60)
                                                                                                                                  GATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTGAGATCTCGCGCCAAGCAACGCGTCCAGCATCTCCACAGGTGCCGTCTCATTAC                   >  SRR19973063.4774622/1‑150 (MQ=60)
                                                                                                                                    TTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTGAGATATCGCGCCAAGCAACGCGTCCAGCATCTCCACAGGTGCCGTCTCATTACCG                 >  SRR19973063.5056749/1‑150 (MQ=60)
                                                                                                                                       ATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTGAGATATCGCGCCAAGCAACGCGTCCAGCATCTCCACAGGTGCCGTCTCATTACCGTGT              >  SRR19973063.3752856/1‑150 (MQ=60)
                                                                                                                                       ATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTGAGATATCGCGCCAAGCAACGCGTCCAGCATCTCCACAGGTGCCGTCTCATTACCGTGT              >  SRR19973063.2073311/1‑150 (MQ=60)
                                                                                                                                       ATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTGAGATATCGCGCCAAGCAACGCGTCCAGCAACTCCACAGGTGACGGCTCATTACCGTGT              >  SRR19973063.3325194/1‑150 (MQ=60)
                                                                                                                                            GCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTGAGATATCGCGCCAAGCAACGCGTCCAGCATCTCCACAGGTGCCGTCTCATTACCGTGTATTCC         <  SRR19973063.2109520/150‑1 (MQ=60)
                                                                                                                                             CTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTGAGATATCGCGCCAAGCAACGCGTCCAGCATCTCCACAGGTGCCGTCTCATTACCGTGTATTCCC        >  SRR19973063.3271762/1‑150 (MQ=60)
                                                                                                                                                TGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTGAGATATCGCGCCAAGCAACGCGTCCAGCATCTCCACAGGTGCCGTCTCATTACCGTGTATTCCCGCT     <  SRR19973063.147468/150‑1 (MQ=60)
                                                                                                                                                   AAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTGAGATATCGCGCCAAGCAACGCGTCCAGCATCTCCACAGGTGCCGTCTCATTACCGTGTATTCCCGCTGAA  >  SRR19973063.812021/1‑150 (MQ=60)
                                                                                                                                                   |                                                                                                                                                     
TGTAGATCAAGGTGCCAGCGCACAGATTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGTTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGCAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTGAGATATCGCGCCAAGCAACGCGTCCAGCATCTCCACAGGTGCCGTCTCATTACCGTGTATTCCCGCTGAA  >  CP009273/1820741‑1821037

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑