Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F434 I0 R1 1130 215.9 7031662 97.1% 6827743 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 390,551 T→G 100% L197R (CTG→CGG)  yaiV → putative transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273390,5510TG98.4% 819.7 / ‑2.1 203L197R (CTG→CGG) yaiVputative transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base T (3/1);  new base G (96/103);  total (99/104)
Fisher's exact test for biased strand distribution p-value = 3.59e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 205 total aligned reads displayed.

ATGATTGACTGGAATGAAGAATTGCGATCACGTATTGGTGTGATGAATTATATCCATCAACGTACACGCATATCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACTGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCAACGGTTTATTCCCGCTTAGCTCGGTCACCAGGTCATTGATGCCATCGCTCATATTTTTAAAGCGCGTCAACGGCGAACGAGTGACCACCA  >  CP009273/390406‑390695
                                                                                                                                                 |                                                                                                                                                
 tGATTGACTGGAATGAAGAATTGCGATCACGTATTGGTGTGATGAATTATATCCATCAACGTACACGCATATCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGt                                                                                                                                               >  2:872775/1‑148 (MQ=255)
   aTTGACTGGAATGAAGAATTGCGATCACGTATTGGTGTGATGAATTATATCCATCAACGTACACGCATATCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCg                                                                                                                                             <  2:389825/148‑1 (MQ=255)
     tGACTGGAATGAAGAATTGCGATCACGTATTGGTGTGATGAATTATATCCATCAACGTACACGCATATCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCt                                                                                                                                           <  2:1660864/148‑1 (MQ=255)
        cTGGAATGAAGAATTGCGATCACGTATTGGTGTGATGAATTATATCCATCAACGTACACGCATATCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATc                                                                                                                                        >  2:205647/1‑148 (MQ=255)
        cTGGAATGAAGAATTGCGATCACGTATTGGTGTGATGAATTATATCCATCAACGTACACGCATATCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCATAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATc                                                                                                                                        >  2:2537067/1‑148 (MQ=255)
         tGGAATGAAGAATTGCGATCACGTATTGGTGTGATGAATTATATCCATCAACGTACACGCATATCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCa                                                                                                                                       <  2:1461575/148‑1 (MQ=255)
           gAATGAAGAATTGCGATCACGTATTGGTGTGATGAATTATATCCATCAACGTACACGCATATCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAAc                                                                                                                                     >  1:2720416/1‑148 (MQ=255)
           gAATGAAGAATTGCGATCACGTATTGGTGTGATGAATTATATCCATCAACGTACACGCATATCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAAc                                                                                                                                     <  2:2526404/148‑1 (MQ=255)
            aaTGAAGAATTGCGATCACGTATTGGTGTGATGAATTATATCCATCAACGTACACGCATATCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAAcc                                                                                                                                    >  2:2088703/1‑148 (MQ=255)
             aTGAAGAATTGCGATCACGTATTGGTGTGATGAATTATATCCATCAACGTACACGCATATCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCg                                                                                                                                   >  1:2939341/1‑148 (MQ=255)
             aTGAAGAATTGCGATCACGTATTGGTGTGATGAATTATATCCATCAACGTACACGCATATCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCg                                                                                                                                   <  1:841567/148‑1 (MQ=255)
              tGAAGAATTGCGATCACGTATTGGTGTGATGAATTATATCCATCAACGTACACGCATATCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGt                                                                                                                                  <  2:2233936/148‑1 (MQ=255)
              tGAAGAATTGCGATCACGTATTGGTGTGATGAATTATATCCATCAACGTACACGCATATCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGt                                                                                                                                  >  1:736801/1‑148 (MQ=255)
                 agaaTTGCGATCACGTATTGGTGTGATGAATTATATCCATCAACGTACACGCATATCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTCTCAACCGTTTg                                                                                                                               >  2:3214031/1‑148 (MQ=255)
                 agaaTTGCGATCACGTATTGGTGTGATGAATTATATCCATCAACGTACACGCATATCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTg                                                                                                                               <  1:566822/148‑1 (MQ=255)
                 agaaTTGCGATCACGTATTGGTGTGATGAATTATATCCATCAACGTACACGCATATCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTg                                                                                                                               >  2:3408777/1‑148 (MQ=255)
                  gaaTTGCGATCACGTATTGGTGTGATGAATTATATCCATCAACGTACACGCATATCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGc                                                                                                                              <  1:1516492/148‑1 (MQ=255)
                  gaaTTGCGATCACGTATTGGTGTGATGAATTATATCCATCAACGTACACGCATATCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGc                                                                                                                              <  1:1814276/148‑1 (MQ=255)
                   aaTTGCGATCACGTATTGGTGTGATGAATTATATCCATCAACGTACACGCATATCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATCACGGCACACGGGTCGCTATCAACCGTTTGcc                                                                                                                             <  1:1584116/148‑1 (MQ=255)
                   aaTTGCGATCACGTATTGGTGTGATGAATTATATCCATCAACGTACACGCATATCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGcc                                                                                                                             >  1:2863336/1‑148 (MQ=255)
                     ttGCGATCACGTATTGGTGTGATGAATTATATCCATCAACGTACACGCATATCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCtt                                                                                                                           >  1:2191239/1‑148 (MQ=255)
                     ttGCGATCACGTATTGGTGTGATGAATTATATCCATCAACGTACACGCATATCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCtt                                                                                                                           >  1:2191462/1‑148 (MQ=255)
                         gATCACGTATTGGTGTGATGAATTATATCCATCAACGTACACGCATATCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCaga                                                                                                                       >  2:602466/1‑148 (MQ=255)
                          aTCACGTATTGGTGTGATGAATTATATCCATCAACGTACACGCATATCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCagag                                                                                                                      >  1:127883/1‑148 (MQ=255)
                          aTCACGTATTGGTGTGATGAATTATATCCATCAACGTACACGCATATCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCagag                                                                                                                      >  1:2815547/1‑148 (MQ=255)
                            cACGTATTGGTGTGATGAATTATATCCATCAACGTACACGCATATCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTa                                                                                                                    >  1:2528912/1‑148 (MQ=255)
                            cACGTATTGGTGTGATGAATTATATCCATCAACGTACACGCATATCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTa                                                                                                                    >  1:3081771/1‑148 (MQ=255)
                            cACGTATTGGTGTGATGAATTATATCCATCAACGTACACGCATATCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTa                                                                                                                    <  2:2330453/148‑1 (MQ=255)
                             aCGTATTGGTGTGATGAATTATATCCATCAACGTACACGCATATCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTAt                                                                                                                   <  2:3459507/148‑1 (MQ=255)
                             aCGTATTGGTGTGATGAATTATATCCATCAACGTACACGCATATCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTAt                                                                                                                   >  2:24031/1‑148 (MQ=255)
                              cGTATTGGTGTGATGAATTATATCCATCAACGTACACGCATATCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTAtt                                                                                                                  >  2:3260186/1‑148 (MQ=255)
                                tATTGGTGTGATGAATTATATCCATCAACGTACACGCATATCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTaa                                                                                                                <  2:1357018/148‑1 (MQ=255)
                                 aTTGGTGTGATGAATTATATCCATCAACGTACACGCATATCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAAt                                                                                                               >  1:3093899/1‑148 (MQ=255)
                                   tGGTGTGATGAATTATATCCATCAACGTACACGCATATCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCa                                                                                                             >  1:206495/1‑148 (MQ=255)
                                     gtgtGATGAATTATATCCATCAACGTACACGCATATCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAgg                                                                                                           <  2:2959421/148‑1 (MQ=255)
                                      tgtgATGAATTATATCCATCAACGTACACGCATATCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGa                                                                                                          >  2:536469/1‑148 (MQ=255)
                                         gatgaATTATATCCATCAACGTACACGCATATCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGAcgc                                                                                                       >  1:1164939/1‑148 (MQ=255)
                                         gatgaATTATATCCATCAACGTACACGCATATCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGAcgc                                                                                                       >  1:1724362/1‑148 (MQ=255)
                                           tgaATTATATCCATCAACGTACACGCATATCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCgg                                                                                                     <  1:26066/148‑1 (MQ=255)
                                             aaTTATATCCATCAACGTACACGCATATCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCgggg                                                                                                   <  2:3496808/148‑1 (MQ=255)
                                               ttATATCCATCAACGTACACGCATATCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGAt                                                                                                 >  2:1755130/1‑148 (MQ=255)
                                                tataTCCATCAACGTACACGCATATCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATa                                                                                                >  2:2010857/1‑148 (MQ=255)
                                                tataTCCATCAACGTACACGCATATCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATa                                                                                                <  2:2933953/148‑1 (MQ=255)
                                                    tCCATCAACGTACACGCATATCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCa                                                                                            <  2:3121075/148‑1 (MQ=255)
                                                    tCCATCAACGTACACGCATATCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCa                                                                                            >  1:1320994/1‑148 (MQ=255)
                                                     ccATCAACGTACACGCATATCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCaa                                                                                           >  1:2873279/1‑148 (MQ=255)
                                                     ccATCAACGTACACGCATATCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCaa                                                                                           <  1:2135925/148‑1 (MQ=255)
                                                        tCAACGTACACGCATATCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCAACgg                                                                                        <  1:1710527/148‑1 (MQ=255)
                                                        tCAACGTACACGCATATCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCAACgg                                                                                        <  2:1327957/148‑1 (MQ=255)
                                                         cAACGTACACGCATATCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCAACGGt                                                                                       >  2:604563/1‑148 (MQ=255)
                                                          aaCGTACACGCATATCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCTTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCAACGGtt                                                                                      <  2:206936/148‑1 (MQ=255)
                                                            cGTACACGCATATCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCAACGGTTTa                                                                                    >  1:322517/1‑148 (MQ=255)
                                                              tACACGCATATCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCAACGGTTTAtt                                                                                  <  2:1516957/148‑1 (MQ=255)
                                                               acacGCATATCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCAACGGTTTATTc                                                                                 <  1:3175822/148‑1 (MQ=255)
                                                                cacGCATATCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCAACGGTTTATTcc                                                                                >  1:1346075/1‑148 (MQ=255)
                                                                   gCATATCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGAGGCGGGGATAACCAACGGTTTATTCCCGc                                                                             >  2:3499391/1‑148 (MQ=255)
                                                                    cATATCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCAACGGTTTATTCCCGCt                                                                            <  2:3384812/148‑1 (MQ=255)
                                                                     atatCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCAACGGTTTATTCCCGCtt                                                                           >  2:693082/1‑148 (MQ=255)
                                                                     atatCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCAACGGTTTATTCCCGCtt                                                                           <  1:489214/148‑1 (MQ=255)
                                                                       atCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCAACGGTTTATTCCCGCTTAg                                                                         <  1:3342184/148‑1 (MQ=255)
                                                                        tCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCAACGGTTTATTCCCGCTTAGc                                                                        >  1:2231138/1‑148 (MQ=255)
                                                                        tCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCAACGGTTTATTCCCGCTTAGc                                                                        >  1:2908700/1‑148 (MQ=255)
                                                                         cgcgTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCAACGGTTTATTCCCGCTTAGCt                                                                       >  1:53096/1‑148 (MQ=255)
                                                                            gTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCAACGGTTTATTCCCGCTTAGCTCgg                                                                    <  1:1252546/148‑1 (MQ=255)
                                                                              tCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCAACGGTTTATTCCCGCTTAGCTCGGTc                                                                  >  2:1922144/1‑148 (MQ=255)
                                                                                 gtcgtcGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCAACGGTTTATTCCCGCTTAGCTCGGTCAcc                                                               >  2:1712519/1‑148 (MQ=255)
                                                                                  tcgtcgCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCAACGGTTTATTCCCGCTTAGCTCGGTCACCa                                                              >  1:2469157/1‑148 (MQ=255)
                                                                                   cgtcgCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCAACGGTTTATTCCCGCTTAGCTCGGTCACCAg                                                             >  2:1656933/1‑148 (MQ=255)
                                                                                      cgCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGACATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCAACGGTTTATTCCCGCTTAGCTCGGTCACCAGGTc                                                          <  2:1704631/148‑1 (MQ=255)
                                                                                      cgCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCAACGGTTTATTCCCGCTTAGCTCGGTCACCAGGTc                                                          >  1:1035965/1‑148 (MQ=255)
                                                                                         aGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCAACGGTTTATTCCCGCTTAGCTCGGTCACCAGGTCAtt                                                       >  1:2036013/1‑148 (MQ=255)
                                                                                          gAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCAACGGTTTATTCCCGCTTAGCTCGGTCACCAGGTCATTg                                                      <  2:768574/148‑1 (MQ=255)
                                                                                          gAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCAACGGTTTATTCCCGCTTAGCTCGGTCACCAGGTCATTg                                                      <  1:430043/148‑1 (MQ=255)
                                                                                           aaGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCAACGGTTTATTCCCGCTTAGCTCGGTCACCAGGTCATTGa                                                     >  2:1948412/1‑148 (MQ=255)
                                                                                              ttCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCAACGGTTTATTCCCGCTTAGCTCGGTCACCAGGTCATTGATGc                                                  >  1:639988/1‑148 (MQ=255)
                                                                                                ctcGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCAACGGTTTATTCCCGCTTAGCTCGGTCACCAGGTCATTGATGCCa                                                <  2:125680/148‑1 (MQ=255)
                                                                                                       ctTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCAACGGTTTATTCCCGCTTAGCTCGGTCACCAGGTCATTGATGCCATCGCTCa                                         >  1:1977134/1‑148 (MQ=255)
                                                                                                         ttGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACTGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCAACGGTTTATTCCCGCTTAGCTCGGTCACCAGGTCATTGATGCCATCGCTCata                                       >  1:3486396/1‑148 (MQ=255)
                                                                                                          tGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCAACGGTTTATTCCCGCTTAGCTCGGTCACCAGGTCATTGATGCCATCGCTCatat                                      <  1:2342049/148‑1 (MQ=255)
                                                                                                              tAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCAACGGTTTATTCCCGCTTAGCTCGGTCACCAGGTCATTGATGCCATCGCTCATAttttt                                  >  1:3208086/1‑148 (MQ=255)
                                                                                                               aaaGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCAACGGTTTATTCCCGCTTAGCTCGGTCACCAGGTCATTGATGCCATCGCTCATATTTTTa                                 >  2:506360/1‑148 (MQ=255)
                                                                                                                aaGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCAACGGTTTATTCCCGCTTAGCTCGGTCACCAGGTCATTGATGCCATCGCTCATATTTTTaa                                <  2:2752061/148‑1 (MQ=255)
                                                                                                                  ggcggcTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCAACGGTTTATTCCCGCTTAGCTCGGTCACCAGGTCATTGATGCCATCGCTCATATTTTTAAAg                              >  1:3443253/1‑148 (MQ=255)
                                                                                                                    cggcTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCAACGGTTTATTCCCGCTTAGCTCGGTCACCAGGTCATTGATGCCATCGCTCATATTTTTAAAgcg                            <  2:1794815/148‑1 (MQ=255)
                                                                                                                          tatCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCAACGGTTTATTCCCGCTTAGCTCGGTCACCAGGTCATTGATGCCATCGCTCATATTTTTAAAGCGCGTCaa                      <  1:988646/148‑1 (MQ=255)
                                                                                                                          tatCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCAACGGTTTATTCCCGCTTAGCTCGGTCACCAGGTCATTGATGCCATCGCTCATATTTTTAAAGCGCGTCaa                      <  2:3245774/148‑1 (MQ=255)
                                                                                                                          tatCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCAACGGTTTATTCCCGCTTAGCTCGGTCACCAGGTCATTGATGCCATCGCTCATATTTTTAAAGCGCGTCaa                      >  2:1413503/1‑148 (MQ=255)
                                                                                                                           atCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCAACGGTTTATTCCCGCTTAGCTCGGTCACCAGGTCATTGATGCCATCGCTCATATTTTTAAAGCGCGTCAAc                     <  1:1421306/148‑1 (MQ=255)
                                                                                                                            tCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCAACGGTTTATTCCCGCTTAGCTCGGTCACCAGGTCATTGATGCCATCGCTCATATTTTTAAAGCGCGTCAACg                    <  2:2761779/148‑1 (MQ=255)
                                                                                                                            tCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCAACGGTTTATTCCCGCTTAGCTCGGTCACCAGGTCATTGATGCCATCGCTCATATTTTTAAAGCGCGTCAACg                    >  1:3141687/1‑148 (MQ=255)
                                                                                                                              gAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCAACGGTTTATTCCCGCTTATCTCGGTCACCAGGTCATTGATGCCATCGCTCATATTTTTAAAGCGCGTCAACGGc                  <  2:127883/148‑1 (MQ=255)
                                                                                                                                aaTGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCAACGGTTTATTCCCGCTTAGCTCGGTCACCAGGTCATTGATGCCATCGCTCATATTTTTAAAGCGCGTCAACGGCGa                <  2:2190437/148‑1 (MQ=255)
                                                                                                                                aaTGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCAACGGTTTATTCCCGCTTAGCTCGGTCACCAGGTCATTGATGCCATCGCTCATATTTTTAAAGCGCGTCAACGGCGa                <  1:420610/148‑1 (MQ=255)
                                                                                                                                aaTGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCAACGGTTTATTCCCGCTTAGCTCGGTCACCAGGTCATTGATGCCATCGCTCATATTTTTAAAGCGCGTCAACGGCGa                <  1:141018/148‑1 (MQ=255)
                                                                                                                                    aaTAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCAACGGTTTATTCCCGCTTAGCTCGGTCACCAGGTCATTGATGCCATCGCTCATATTTTTAAAGCGCGTCAACGGCGAACGa            <  2:2856816/148‑1 (MQ=255)
                                                                                                                                        aaGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCAACGGTTTATTCCCGCTTAGCTCGGTCACCAGGTCATTGATGCCATCGCTCATATTTTTAAAGCGCGTCAACGGCGAACGAGTGa        <  1:24031/148‑1 (MQ=255)
                                                                                                                                         aGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCAACGGTTTATTCCCGCTTAGCTCGGTCACCAGGTCATTGATGCCATCGCTCATATTTTTAAAGCGCGTCAACGGCGAACGAGTGac       <  1:1135265/148‑1 (MQ=255)
                                                                                                                                          ggCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCAACGGTTTATTCCCGCTTAGCTCGGTCACCAGGTCATTGATGCCATCGCTCATATTTTTAAAGCGCGTCAACGGCGAACGAGTGacc      >  1:2551717/1‑148 (MQ=255)
                                                                                                                                           gCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCAACGGTTTATTCCCGCTTAGCTCGGTCACCAGGTCATTGATGCCATCGCTCATATTTTTAAAGCGCGTCAACGGCGAACGAGTGacca     >  2:2279442/1‑148 (MQ=255)
                                                                                                                                              aaCGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCAACGGTTTATTCCCGCTTAGCTCGGTCACCAGGTCATTGATGCCATCGCTCATATTTTTAAAGCGCGTCAACGGCGAACGAGTGAccacca  >  2:4741/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
ATGATTGACTGGAATGAAGAATTGCGATCACGTATTGGTGTGATGAATTATATCCATCAACGTACACGCATATCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACTGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCAACGGTTTATTCCCGCTTAGCTCGGTCACCAGGTCATTGATGCCATCGCTCATATTTTTAAAGCGCGTCAACGGCGAACGAGTGACCACCA  >  CP009273/390406‑390695

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
ATCAATGATTGACTGGAATGAAGAATTGCGATCACGTATTGGTGTGATGAATTATATCCATCAACGTACACGCATATCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACTGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCAACGGTTTATTCCCGCTTAGCTCGGTCACCAGGTCATTGATGCCATCGCTCATATTTTTAAAGCGCGTCAACGGCGAACGAGTGACCACCACA  >  CP009273/390402‑390697
                                                                                                                                                     |                                                                                                                                                  
ATCAATGATTGACTGGAATGAAGAATTGCGATCACGTATTGGTGTGATGAATTATATCCATCAACGTACACGCATATCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACG                                                                                                                                                    <  SRR19973065.1035076/150‑1 (MQ=60)
 TCAATGATTGACTGGAATGAAGAATTGCGATCACGTATTGGTGTGATGAATTATATCCATCAACGTACACGCATATCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGG                                                                                                                                                   <  SRR19973065.2036070/150‑1 (MQ=60)
 TCAATGATTGACTGGAATGAAGAATTGCGATCACGTATTGGTGTGATGAATTATATCCATCAACGTACACGCATATCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGG                                                                                                                                                   <  SRR19973065.1405564/150‑1 (MQ=60)
 TCAATGATTGACTGGAATGAAGAATTGCGATCACGTATTGGTGTGATGAATTATATCCATCAACGTACACGCATATCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGG                                                                                                                                                   <  SRR19973065.2630608/150‑1 (MQ=60)
  CAATGATTGACTGGAATGAAGAATTGCGATCACGTATTGGTGTGATGAATTATATCCATCAACGTACACGCATATCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGG                                                                                                                                                  <  SRR19973065.1438922/150‑1 (MQ=60)
  CAATGATTGACTGGAATGAAGAATTGCGATCACGTATTGGTGTGATGAATTATATCCATCAACGTACACGCATATCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGG                                                                                                                                                  <  SRR19973065.1167881/150‑1 (MQ=60)
   AATGATTGACTGGAATGAAGAATTGCGATCACGTATTGGTGTGATGAATTATATCCATCAACGTACACGCATATCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGT                                                                                                                                                 <  SRR19973065.273163/150‑1 (MQ=60)
     TGATTGACTGGAATGAAGAATTGCGATCACGTATTGGTGTGATGAATTATATCCATCAACGTACACGCATATCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCG                                                                                                                                               <  SRR19973065.509038/150‑1 (MQ=60)
             TGGAATGAAGAATTGCGATCACGTATTGGTGTGATGAATTATATCCATCAACGTACACGCATATCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAAC                                                                                                                                       >  SRR19973065.2737335/1‑150 (MQ=60)
              GGAATGAAGAATTGCGATCACGTATTGGTGTGATGAATTATATCCATCAACGTACACGCATATCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACC                                                                                                                                      >  SRR19973065.2036254/1‑150 (MQ=60)
               GAATGAAGAATTGCGATCACGTATTGGTGTGATGAATTATATCCATCAACGTACACGCATATCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATTAATAAAGGCAAACGGGTCGATATCAACCG                                                                                                                                     >  SRR19973065.2957176/1‑150 (MQ=60)
               GAATGAAGAATTGCGATCACGTATTGGTGTGATGAATTATATCCATCAACGTACACGCATATCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCG                                                                                                                                     >  SRR19973065.2957175/1‑150 (MQ=60)
                AATGAAGAATTGCGATCACGTATTGGTGTGATGAATTATATCCATCAACGTACACGCATATCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGT                                                                                                                                    >  SRR19973065.741727/1‑150 (MQ=60)
                 ATGAAGAATTGCGATCACGTATTGGTGTGATGAATTATATCCATCAACGTACACGCATATCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTT                                                                                                                                   <  SRR19973065.847043/150‑1 (MQ=60)
                  TGAAGAATTGCGATCACGTATTGGTGTGATGAATTATATCCATCAACGTACACGCATATCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTT                                                                                                                                  <  SRR19973065.1172876/150‑1 (MQ=60)
                  TGAAGAATTGCGATCACGTATTGGTGTGATGAATTATATCCATCAACGTACACGCATATCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTT                                                                                                                                  <  SRR19973065.3194424/150‑1 (MQ=60)
                     AGAATTGCGATCACGTATTGGTGTGATGAATTATATCCATCAACGTACACGCATATCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCC                                                                                                                               >  SRR19973065.2880910/1‑150 (MQ=60)
                     AGAATTGCGATCACGTATTGGTGTGATGAATTATATCCATCAACGTACACGCATATCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCC                                                                                                                               <  SRR19973065.570418/150‑1 (MQ=60)
                      TAATTGCGATCACGTATTGGTGTGATGAATTATATCCATCAACGTACACGCATATCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCT                                                                                                                              <  SRR19973065.1526401/150‑1 (MQ=60)
                      GAATTGCGATCACGTATTGGTGTGATGAATTATATCCATCAACGTACACGCATATCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCT                                                                                                                              <  SRR19973065.1826220/150‑1 (MQ=60)
                       AATTGCGATCACGTATTGGTGTGATGAATTATATCCATCAACGTACACGCATATCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATCACGGCACACGGGTCGCTATCAACCGTTTGCCTT                                                                                                                             <  SRR19973065.1594679/150‑1 (MQ=60)
                       AATTGCGATCACGTATTGGTGTGATGAATTATATCCATCAACGTACACGCATATCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTT                                                                                                                             >  SRR19973065.2205484/1‑150 (MQ=60)
                       AATTGCGATCACGTATTGGTGTGATGAATTATATCCATCAACGTACACGCATATCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTT                                                                                                                             >  SRR19973065.2205288/1‑150 (MQ=60)
                       AATTGCGATCACGTATTGGTGTGATGAATTATATCCATCAACGTACACGCATATCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTT                                                                                                                             >  SRR19973065.2205513/1‑150 (MQ=60)
                        ATTGCGATCACGTATTGGTGTGATGAATTATATCCATCAACGTACACGCATATCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATAAACCCTTTTCCTTC                                                                                                                            >  SRR19973065.3404889/1‑150 (MQ=60)
                            CGATCACGTATTGGTGTGATGAATTATATCCATCAACGTACACGCATATCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAG                                                                                                                        >  SRR19973065.2832946/1‑150 (MQ=60)
                            CGATCACGTATTGGTGTGATGAATTATATCCATCAACGTACACGCATATCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAG                                                                                                                        >  SRR19973065.128974/1‑150 (MQ=60)
                            CGATCACGTATTGGTGTGATGAATTATATCCATCAACGTACACGCATATCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGGTTGCCTTCAGAG                                                                                                                        >  SRR19973065.128987/1‑150 (MQ=60)
                              ATCACGTATTGGTGTGATGAATTATATCCATCAACGTACACGCATATCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTCTCAACCGTTTGCCTTCAGAGTA                                                                                                                      >  SRR19973065.773682/1‑150 (MQ=60)
                              ATCACGTATTGGTGTGATGAATTATATCCATCAACGTACACGCATATCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTA                                                                                                                      >  SRR19973065.2544786/1‑150 (MQ=60)
                              ATCACGTATTGGTGTGATGAATTATATCCATCAACGTACACGCATATCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTA                                                                                                                      >  SRR19973065.3100590/1‑150 (MQ=60)
                                 ACGTATTGGTGTGATGAATTATATCCATCAACGTACACGCATATCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTA                                                                                                                   <  SRR19973065.2625213/150‑1 (MQ=60)
                                   GTATTGGTGTGATGAATTATATCCATCAACGTACACGCATATCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAAT                                                                                                                 >  SRR19973065.3112789/1‑150 (MQ=60)
                                   GTATTGGTGTGATGAATTATATCCATCAACGTACACGCATATCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAAT                                                                                                                 >  SRR19973065.3111128/1‑150 (MQ=60)
                                    TATTGGTGTGATGAATTATATCCATCAACGTACACGCATATCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATC                                                                                                                >  SRR19973065.1725932/1‑150 (MQ=60)
                                     ATTGGTGTGATGAATTATATCCATCAACGTACACGCATATCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCA                                                                                                               >  SRR19973065.208064/1‑150 (MQ=60)
                                     ATTGGTGTGATGAATTATATCCATCAACGTACACGCATATCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCA                                                                                                               >  SRR19973065.3239223/1‑150 (MQ=60)
                                           GTGATGAATTATATCCATCAACGTACACGCATATCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGC                                                                                                         >  SRR19973065.1172382/1‑150 (MQ=60)
                                           GTGATGAATTATATCCATCAACGTACACGCATATCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGC                                                                                                         >  SRR19973065.1735804/1‑150 (MQ=60)
                                           GTGATGAATTATATCCATCAACGTACACGCATATCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGC                                                                                                         >  SRR19973065.1289078/1‑150 (MQ=60)
                                           GTGATGAATTATATCCATCAACGTACACGCATATCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGC                                                                                                         >  SRR19973065.2932626/1‑150 (MQ=60)
                                             GATGAATTATATCCATCAACGTACACGCATATCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGG                                                                                                       >  SRR19973065.342623/1‑150 (MQ=60)
                                             GATGAATTATATCCATCAACGTACACGCATATCGCGTTCTGTCGTCGCAGAAGTACTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGG                                                                                                       <  SRR19973065.1427023/150‑1 (MQ=60)
                                               TGAATTATATCCATCAACGTACACGCATATCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGG                                                                                                     <  SRR19973065.26409/150‑1 (MQ=60)
                                               TGAATTATATCCATCAACGTACACGCATATCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGG                                                                                                     <  SRR19973065.26410/150‑1 (MQ=60)
                                                GAATTATATCCATCAACGGACACGCATATCGCGTTCTGTCGTCGCAGAAGGTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGA                                                                                                    <  SRR19973065.1912845/150‑1 (MQ=60)
                                                   TTATATCCATCAACGTACACGCATATCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATTAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAA                                                                                                 <  SRR19973065.1513440/150‑1 (MQ=60)
                                                      TATCCATCAACGTACACGCATATCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCA                                                                                              <  SRR19973065.289220/150‑1 (MQ=60)
                                                      TATCCATCAACGTACACGCATATCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCA                                                                                              >  SRR19973065.1329730/1‑150 (MQ=60)
                                                       ATCCATCAACGTACACGCATATCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCAA                                                                                             >  SRR19973065.2890807/1‑150 (MQ=60)
                                                       ATCCATCAACGTACACGCATATCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCAA                                                                                             >  SRR19973065.2890885/1‑150 (MQ=60)
                                                         CCATCAACGTACACGCATATCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCAACG                                                                                           <  SRR19973065.2150532/150‑1 (MQ=60)
                                                         CCATCAACGTACACGCATATCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCAACG                                                                                           <  SRR19973065.2149671/150‑1 (MQ=60)
                                                           ATCAACGTACACGCATATCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCAACGGT                                                                                         >  SRR19973065.1554336/1‑150 (MQ=60)
                                                            TCAACGTACACGCATATCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCAACGGTT                                                                                        <  SRR19973065.1721910/150‑1 (MQ=60)
                                                              AACGTACACGCATATCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCAACGGTTTA                                                                                      >  SRR19973065.324752/1‑150 (MQ=60)
                                                                CGTACACGCATATCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCAACGGTTTATT                                                                                    <  SRR19973065.2502374/150‑1 (MQ=60)
                                                                  TACACGCATATCGCGTTCTGTCTTCGCAGAAGTTCTCGGTGGTTTGCGTAAAGGCGGCTATATCGAAATGATTAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCAACGGTTTATTCC                                                                                  <  SRR19973065.552952/150‑1 (MQ=60)
                                                                  TACACGCATATCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCAACGGTTTATTCC                                                                                  <  SRR19973065.552714/150‑1 (MQ=60)
                                                                  TACACGCATATCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCAACGGTTTATTCC                                                                                  >  SRR19973065.1354976/1‑150 (MQ=60)
                                                                   ACACGCATATCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCAACGGTTTATTCCC                                                                                 <  SRR19973065.3195021/150‑1 (MQ=60)
                                                                   ACACGCATATCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCAACGGTTTATTCCC                                                                                 <  SRR19973065.1045869/150‑1 (MQ=60)
                                                                      CGCATATCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCAACGGTTTATTCCCGCT                                                                              >  SRR19973065.2106714/1‑150 (MQ=60)
                                                                       GCATATCGCTTTCTGTCGTCGCAGATTTTCTCGCTGCTTTGCGTAAAGGCGGATATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCAACGGTTTATTCCCGCTT                                                                             <  SRR19973065.618544/150‑1 (MQ=60)
                                                                        CATATCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCAACGGTTTATTCCCGCTTA                                                                            >  SRR19973065.351283/1‑150 (MQ=60)
                                                                         ATATCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCAACGGTTTATTCCCGCTTAG                                                                           <  SRR19973065.492521/150‑1 (MQ=60)
                                                                         ATATCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCAACGGTTTATTCCCGCTTAG                                                                           <  SRR19973065.207212/150‑1 (MQ=60)
                                                                          TATCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCAACGGTTTATTCCCGCTTAGC                                                                          >  SRR19973065.2245336/1‑150 (MQ=60)
                                                                          TATCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCAACGGTTTATTCCCGCTTAGC                                                                          >  SRR19973065.2926411/1‑150 (MQ=60)
                                                                           ATCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCAACGGTTTATTCCCGCTTAGCT                                                                         >  SRR19973065.53680/1‑150 (MQ=60)
                                                                           ATCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCAACGGTTTATTCCCGCTTAGCT                                                                         <  SRR19973065.3362604/150‑1 (MQ=60)
                                                                            TCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCAACGGTTTATTCCCGCTTAGCTC                                                                        <  SRR19973065.1632102/150‑1 (MQ=60)
                                                                                GTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCAACGGTTTATTCCCGCTTAGCTCGGTC                                                                    <  SRR19973065.1260791/150‑1 (MQ=60)
                                                                                GTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCATAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCAACGGTTTATTCCCGCTTAGCTCGGTC                                                                    <  SRR19973065.2552978/150‑1 (MQ=60)
                                                                                    TGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCAACGGTTTATTCCCGCTTAGCTCGGTCACCA                                                                >  SRR19973065.2484708/1‑150 (MQ=60)
                                                                                       CGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCAACGGTTTATTCCCGCTTAGCTCGGTCACCAGGT                                                             >  SRR19973065.3214801/1‑150 (MQ=60)
                                                                                       CGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCAACGGGTTATTCCCGCTTAGCTCGGTCACCAGGT                                                             >  SRR19973065.3249253/1‑150 (MQ=60)
                                                                                        GTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCAACGGTTTATTCCCGCTTAGCTCGGTCACCAGGTC                                                            >  SRR19973065.1042826/1‑150 (MQ=60)
                                                                                         TCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCAACGGTTTATTCCCGCTTAGCTCGGTCACCAGGTCA                                                           <  SRR19973065.1383227/150‑1 (MQ=60)
                                                                                          CGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCAACGGTTTATTCCCGCTTAGCTCGGTCACCAGGTCAT                                                          <  SRR19973065.2479080/150‑1 (MQ=60)
                                                                                           GCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCAACGGTTTATTCCCGCTTAGCTCGGTCACCAGGTCATT                                                         >  SRR19973065.2049061/1‑150 (MQ=60)
                                                                                              GAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCAACGGTTTATTCCCGCTTAGCTCGGTCACCAGGTCATTGAT                                                      <  SRR19973065.433027/150‑1 (MQ=60)
                                                                                              GAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCAACGGTTTATTCCCGCTTAGCTCGGTCACCAGGTCATTGAT                                                      <  SRR19973065.433028/150‑1 (MQ=60)
                                                                                                AGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCAACGGTTTATTCCCGCTTAGCTCGGTCACCAGGTCATTGATGC                                                    <  SRR19973065.2839528/150‑1 (MQ=60)
                                                                                                AGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCAACGGTTTATTCCCGCTTAGCTCGGTCACCAGGTCATTGATGC                                                    >  SRR19973065.644185/1‑150 (MQ=60)
                                                                                                AGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCAACGGTTTATTCCCGCTTAGCTCGGTCACCAGGTCATTGATGC                                                    >  SRR19973065.2778941/1‑150 (MQ=60)
                                                                                                         TGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCAACGGTTTATTCCCGCTTAGCTCGGTCACCAGGTCATTGATGCCATCGCTCA                                           >  SRR19973065.1989998/1‑150 (MQ=60)
                                                                                                          GCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACTGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCAACGGTTTATTCCCGCTTAGCTCGGTCACCAGGTCATTGATGCCATCGCTCAT                                          <  SRR19973065.2227003/150‑1 (MQ=60)
                                                                                                           CTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACTGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCAACGGTTTATTCCCTCTTAGCTCGGTCACCAGGTCATTGATGCCATCGCTCATA                                         >  SRR19973065.3507563/1‑150 (MQ=60)
                                                                                                           CTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACTGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCAACGGTTTATTCCCGCTTAGCTCGGTCACCAGGTCATTGATGCCATCGCTCATA                                         >  SRR19973065.3507599/1‑150 (MQ=60)
                                                                                                              TGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCATGACGCGGGGATAACCAACGGTTTATTCCCGCTTAGCTCGGTCACCAGGTCATTGATGCCATCGCTCATATTT                                      >  SRR19973065.68397/1‑150 (MQ=60)
                                                                                                              TGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCAACGGTTTATTCCCGCTTAGCTCGGTCACCAGGTCATTGATGCCATCGCTCATATTT                                      <  SRR19973065.2356667/150‑1 (MQ=60)
                                                                                                                CGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCAACGGTTTATTCCCGCTTAGCTCGGTCACCAGGTCATTGATGCCATCGCTCATATTTTT                                    >  SRR19973065.3227392/1‑150 (MQ=60)
                                                                                                                    AAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCAACGGTTTATTCCCGCTTAGCTCGGTCACCAGGTCATTGATGCCATCGCTCATATTTTTAAAG                                >  SRR19973065.3464249/1‑150 (MQ=60)
                                                                                                                             ATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCAACGGTTTATTCCCGCTTAGCTCGGTCACCAGGTCATTGATGCCATCGCTCATATTTTTAAAGCGCGTCAAC                       <  SRR19973065.1776350/150‑1 (MQ=60)
                                                                                                                              TATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCAACGGTTTATTCCCGCTTAGCTCGGTCACCAGGTCATTGATGCCATCGCTCATATTTTTAAAGCGCGTCAACG                      <  SRR19973065.995206/150‑1 (MQ=60)
                                                                                                                              TATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCAACGGTTTATTCCCGCTTAGCTCGGTCACCAGGTCATTGATGCCATCGCTCATATTTTTAAAGCGCGTCAACG                      <  SRR19973065.995214/150‑1 (MQ=60)
                                                                                                                              TATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCAACGGTTTATTCCCGCTTAGCTCGGTCACCAGGTCATTGATGCCATCGCTCATATTTTTAAAGCGCGTCAACG                      <  SRR19973065.996608/150‑1 (MQ=60)
                                                                                                                              TATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCAACGGTTTATTCCCGCTTAGCTCGGTCACCAGGTCATTGATGCCATCGCTCATATTTTTAAAGCGCGTCAACG                      >  SRR19973065.3160782/1‑150 (MQ=60)
                                                                                                                              TATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCAACGGTTTATTCCCGCTTAGCTCGGTCACCAGGTCATTGATGCCATCGCTCATATTTTTAAAGCGCGTCAACG                      >  SRR19973065.3159970/1‑150 (MQ=60)
                                                                                                                               ATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCAACGGTTTATTCCCGCTTAGCTCGGTCACCAGGTCATTGATGCCATCGCTCATATTTTTAAAGCGCGTCAACGG                     <  SRR19973065.2230989/150‑1 (MQ=60)
                                                                                                                               ATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCAACGGTTTATTCCCGCTTAGCTCGGTCACCAGGTCATTGATGCCATCGCTCATATTTTTAAAGCGCGTCAACGG                     <  SRR19973065.1430580/150‑1 (MQ=60)
                                                                                                                                    AATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCAACGGTTTATTCCCGCTTAGCTCGGTCACCAGGTCATTGATGCCATCGCTCATATTTTTAAAGCGCGTCAACGGCGAAC                <  SRR19973065.423546/150‑1 (MQ=60)
                                                                                                                                    AATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCAACGGTTTATTCCCGCTTAGCTCGGTCACCAGGTCATTGATGCCATCGCTCATATTTTTAAAGCGCGTCAACGGCGAAC                <  SRR19973065.142220/150‑1 (MQ=60)
                                                                                                                                     ATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCAACGGTTTATTCCCGCTTAGCTCGGTCACCAGGTCATTGATGCCATCGCTCATATTTTTAAAGCGCGTCAACGGCGAACG               <  SRR19973065.2287128/150‑1 (MQ=60)
                                                                                                                                       GAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCAACGGTTTATTCCCGCTTAGCTCGGTCACCAGGTCATTGATGCCATCGCTCATATTTTTAAAGCGCGTCAACGGCGAACGAG             <  SRR19973065.492077/150‑1 (MQ=60)
                                                                                                                                        AATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCAACGGTTTATTCCCGCTTAGCTCGGTCACCAGGTCATTGATGCCATCGCTCATATTTTTAAAGCGCGTCAACGGCGAACGAGT            <  SRR19973065.2357537/150‑1 (MQ=60)
                                                                                                                                            AAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCAACGGTTTATTCCCGCTTAGCTCGGTCACCAGGTCATTGATGCCATCGCTCATATTTTTAAAGCGCGTCAACGGCGAACGAGTGACC        >  SRR19973065.2567687/1‑150 (MQ=60)
                                                                                                                                            AAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCAACGGTTTATTCCCGCTTAGCTCGGTCACCAGGTCATTGATGCCATCGCTCATATTTTTAAAGCGCGTCAACGGCGAACGAGTGACC        <  SRR19973065.24351/150‑1 (MQ=60)
                                                                                                                                             AGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCAACGGTTTATTCCCGCTTAGCTCGGTCACCAGGTCATTGATGCCATCGCTCATATTTTTAAAGCGCGTCAACGGCGAACGAGTGACCA       <  SRR19973065.1142624/150‑1 (MQ=60)
                                                                                                                                                CAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCAACGGTTTATTCCCGCTTAGCTCGGTCACCAGGTCATTGATGCCATCGCTCATATTTTTAAAGCGCGTCAACGGCGAACGAGTGACCACCA    >  SRR19973065.1142640/1‑150 (MQ=60)
                                                                                                                                                  AACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCAACGGTTTATTCCCGCTTAGCTCGGTCACCAGGTCATTGATGCCATCGCTCATATTTTTAAAGCGCGTCAACGGCGAACGAGTGACCACCACA  <  SRR19973065.134025/150‑1 (MQ=60)
                                                                                                                                                     |                                                                                                                                                  
ATCAATGATTGACTGGAATGAAGAATTGCGATCACGTATTGGTGTGATGAATTATATCCATCAACGTACACGCATATCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACTGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCAACGGTTTATTCCCGCTTAGCTCGGTCACCAGGTCATTGATGCCATCGCTCATATTTTTAAAGCGCGTCAACGGCGAACGAGTGACCACCACA  >  CP009273/390402‑390697

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑