Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F434 I0 R1 1130 215.9 7031662 97.1% 6827743 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 444,489 G→A 100% R537C (CGT→TGT)  cyoB ← cytochrome o ubiquinol oxidase subunit I

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273444,4890GA99.2% 843.4 / ‑2.4 256R537C (CGT→TGT) cyoBcytochrome o ubiquinol oxidase subunit I
Reads supporting (aligned to +/- strand):  ref base G (2/0);  new base A (132/122);  total (134/122)
Fisher's exact test for biased strand distribution p-value = 4.99e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.90e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 256 total aligned reads displayed.

ATATGAATTTCTTCATAGTGGTCAGGCTTTTTATACGCTTCGCCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACGGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCAGAGAATACCCAGCGCAATCAGTACTGCACCGCTGGCTGCAATCATCAGCATGGTGTGGAACTG  >  CP009273/444344‑444630
                                                                                                                                                 |                                                                                                                                             
 tatGAATTTCTTCATAGTGGTCAGGCTTTTTATACGCTTCGCCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGcc                                                                                                                                            >  2:1037112/1‑148 (MQ=255)
     aaTTTCTTCATAGTGGTCAGGCTTTTTATACGCTTCGCCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCAccc                                                                                                                                        >  1:3450328/1‑148 (MQ=255)
      aTTTCTTCATAGTGGTCAGGCTTTTTATACGCTTCGCCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCAcccc                                                                                                                                       >  2:3367696/1‑148 (MQ=255)
      aTTTCTTCATAGTGGTCAGGCTTTTTATACGCTTCGCCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCAcccc                                                                                                                                       >  1:734370/1‑148 (MQ=255)
        ttcttcATAGTGGTCAGGCTTTTTATACGCTTCGCCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCAc                                                                                                                                     >  1:219577/1‑148 (MQ=255)
        ttcttcATAGTGGTCAGGCTTTTTATACGCTTCGCCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCAc                                                                                                                                     <  1:1592829/148‑1 (MQ=255)
                aGTGGTCAGGCTTTTTATACGCTTCGCCTTTCTCTTTCATTTCCCAGAATGCATCTCGTTCGTGTACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGATGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGcc                                                                                                                             <  2:2087228/148‑1 (MQ=255)
                  tGGTCAGGCTTTTTATACGCTTCGCCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAg                                                                                                                           >  1:2251445/1‑148 (MQ=255)
                    gTCAGGCTTTTTATACGCTTCGCCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTc                                                                                                                         <  2:2511117/148‑1 (MQ=255)
                      cAGGCTTTTTATACGCTTCGCCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAg                                                                                                                       >  1:588357/1‑148 (MQ=255)
                         gCTTTTTATACGCTTCGCCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTc                                                                                                                    >  2:1689149/1‑148 (MQ=255)
                            ttttATACGCTTCGCCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACg                                                                                                                 <  1:3012286/148‑1 (MQ=255)
                             tttATACGCTTCGCCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGTTAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACgg                                                                                                                <  2:200270/148‑1 (MQ=255)
                             tttATACGCTTCGCCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACgg                                                                                                                >  1:1190318/1‑148 (MQ=255)
                              ttATACGCTTCGCCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGt                                                                                                               <  2:241854/148‑1 (MQ=255)
                               tataCGCTTCGCCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGtt                                                                                                              <  2:2614616/148‑1 (MQ=255)
                                ataCGCTTCGCCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTc                                                                                                             <  2:913654/148‑1 (MQ=255)
                                 taCGCTTCGCCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCt                                                                                                            <  2:1339019/148‑1 (MQ=255)
                                  aCGCTTCGCCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTg                                                                                                           >  1:2368839/1‑148 (MQ=255)
                                      ttCGCCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTggtcg                                                                                                       >  1:1200210/1‑148 (MQ=255)
                                       tCGCCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTggtcgc                                                                                                      >  1:856800/1‑148 (MQ=255)
                                       tCGCCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTggtcgc                                                                                                      >  1:1635881/1‑148 (MQ=255)
                                         gCCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTggtcgcgg                                                                                                    >  1:1148735/1‑148 (MQ=255)
                                          ccTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTggtcgcggt                                                                                                   <  1:1760560/148‑1 (MQ=255)
                                          ccTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTggtcgcggt                                                                                                   <  1:2551792/148‑1 (MQ=255)
                                             ttCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTggtcgcggtcgc                                                                                                >  2:2216200/1‑148 (MQ=255)
                                              tctcTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGgtcgcggtcgcg                                                                                               >  1:217914/1‑148 (MQ=255)
                                              tctcTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGgtcgcggtcgcg                                                                                               >  2:404483/1‑148 (MQ=255)
                                               ctctTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGa                                                                                              <  1:853214/148‑1 (MQ=255)
                                                 ctTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAAt                                                                                            >  2:2664073/1‑148 (MQ=255)
                                                 ctTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAAt                                                                                            >  2:2293202/1‑148 (MQ=255)
                                                    tCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGa                                                                                         >  1:2585067/1‑148 (MQ=255)
                                                     cATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGaa                                                                                        <  1:141936/148‑1 (MQ=255)
                                                     cATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGaa                                                                                        <  1:1003269/148‑1 (MQ=255)
                                                     cATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGaa                                                                                        <  1:1775497/148‑1 (MQ=255)
                                                         tCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACCCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGt                                                                                    <  2:3406273/148‑1 (MQ=255)
                                                          cccAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTa                                                                                   >  1:48769/1‑148 (MQ=255)
                                                              gAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATc                                                                               <  1:2787928/148‑1 (MQ=255)
                                                               aaTGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCt                                                                              >  1:787194/1‑148 (MQ=255)
                                                                 tGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGa                                                                            <  2:3300415/148‑1 (MQ=255)
                                                                  gCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGaa                                                                           <  2:353989/148‑1 (MQ=255)
                                                                    aTCACGTTCGTGAACGTGTGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATa                                                                         <  1:2707622/148‑1 (MQ=255)
                                                                    aTCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATa                                                                         <  2:1090052/148‑1 (MQ=255)
                                                                     tCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATaa                                                                        <  1:947265/148‑1 (MQ=255)
                                                                      cACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGACTAGAAACGTACATCTGAATAAc                                                                       >  2:1241481/1‑148 (MQ=255)
                                                                      cACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAAc                                                                       >  1:830774/1‑148 (MQ=255)
                                                                      cACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAAc                                                                       >  1:1207113/1‑148 (MQ=255)
                                                                       aCGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACg                                                                      <  2:1235429/148‑1 (MQ=255)
                                                                        cGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGa                                                                     >  2:3241216/1‑148 (MQ=255)
                                                                        cGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGa                                                                     >  2:890938/1‑148 (MQ=255)
                                                                          ttCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAgg                                                                   <  1:324515/148‑1 (MQ=255)
                                                                           tCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGc                                                                  >  2:1668843/1‑148 (MQ=255)
                                                                           tCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGc                                                                  >  1:1883106/1‑148 (MQ=255)
                                                                           tCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGc                                                                  <  1:551008/148‑1 (MQ=255)
                                                                           tCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGc                                                                  >  2:1201747/1‑148 (MQ=255)
                                                                             gTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCag                                                                <  2:2921278/148‑1 (MQ=255)
                                                                                 aCGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCAGAGaa                                                            <  2:1839370/148‑1 (MQ=255)
                                                                                  cGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCAGAGAAt                                                           >  2:1675776/1‑148 (MQ=255)
                                                                                   gTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCAGAGAATa                                                          <  1:1455276/148‑1 (MQ=255)
                                                                                           cTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCAGAGAATACCCAgcgc                                                  >  2:1791769/1‑148 (MQ=255)
                                                                                             aCGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCAGAGAATACCCAGCGCaa                                                <  1:2127695/148‑1 (MQ=255)
                                                                                                 cAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCAGAGAATACCCAGCGCAATCAg                                            >  2:24357/1‑148 (MQ=255)
                                                                                                 cAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCAGAGAATACCCAGCGCAATCAg                                            >  2:264233/1‑148 (MQ=255)
                                                                                                   aaGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCAGAGAATACCCAGCGCAATCAGTa                                          <  1:2596639/148‑1 (MQ=255)
                                                                                                   aaGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCAGAGAATACCCAGCGCAATCAGTa                                          <  2:1923374/148‑1 (MQ=255)
                                                                                                     gTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCAGAGAATACCCAGCGCAATCAGTACt                                        >  1:1542387/1‑148 (MQ=255)
                                                                                                       tataGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCAGAGAATACCCAGCGCAATCAGTACTGc                                      <  1:2949660/148‑1 (MQ=255)
                                                                                                        ataGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCAGAGAATACCCAGCGCAATCAGTACTGCa                                     <  2:3271264/148‑1 (MQ=255)
                                                                                                         taGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCAGAGAATACCCAGCGCAATCAGTACTGCAc                                    >  2:596339/1‑148 (MQ=255)
                                                                                                            aaCGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCAGAGAATACCCAGCGCAATCAGTACTGCACCgc                                 <  1:2892482/148‑1 (MQ=255)
                                                                                                            aaCGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCAGAGAATACCCAGCGCAATCAGTACTGCACCgc                                 >  1:3351519/1‑148 (MQ=255)
                                                                                                            aaCGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCAGAGAATACCCAGCGCAATCAGTACTGCACCgc                                 >  1:1527020/1‑148 (MQ=255)
                                                                                                            aaCGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCAGAGAATACCCAGCGCAATCAGTACTGCACCgc                                 <  1:755921/148‑1 (MQ=255)
                                                                                                                 aGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCAGAGAATACCCAGCGCAATCAGTACTGCACCgctggct                            <  2:3433976/148‑1 (MQ=255)
                                                                                                                 aGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCAGAGAATACCCAGCGCAATCAGTACTGCACCgctggct                            >  2:2929420/1‑148 (MQ=255)
                                                                                                                  ggCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCAGAGAATACCCAGCGCAATCAGTACTGCACCgctggctg                           <  1:2540765/148‑1 (MQ=255)
                                                                                                                     ggggAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCAGAGAATACCCAGCGCAATCAGTACTGCACCGCTGGCTGCaa                        <  2:2251445/148‑1 (MQ=255)
                                                                                                                        gAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCAGAGAATACCCAGCGCAATCAGTACTGCACCGCTGGCTGCAatca                     <  1:2084700/148‑1 (MQ=255)
                                                                                                                         aaGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCAGAGAATACCCAGCGCAATCAGTACTGCACCGCTGGCTGCAatcat                    >  2:223656/1‑148 (MQ=255)
                                                                                                                         aaGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCAGAGAATACCCAGCGCAATCAGTACTGCACCGCTGGCTGCAatcat                    <  2:734399/148‑1 (MQ=255)
                                                                                                                         aaGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCAGAGAATACCCAGCGCAATCAGTACTGCACCGCTGGCTGCAatcat                    <  2:734354/148‑1 (MQ=255)
                                                                                                                         aaGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCAGAGAATACCCAGCGCAATCAGTACTGCACCGCTGGCTGCAatcat                    <  1:1889414/148‑1 (MQ=255)
                                                                                                                          agagGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCAGAGAATACCCAGCGCAATCAGTACTGCACCGCTGGCTGCAatcatc                   <  2:1226879/148‑1 (MQ=255)
                                                                                                                           gagGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCAGAGAATACCCAGCGCAATCAGTACTGCACCGCTGGCTGCAAtcatca                  >  2:384954/1‑148 (MQ=255)
                                                                                                                           gagGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCAGAGAATACCCAGCGCAATCAGTACTGCACCGCTGGCTGCAAtcatca                  >  1:2452332/1‑148 (MQ=255)
                                                                                                                            agGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCAGAGAATACCCAGCGCAATCAGTACTGCACCGCTGGCTGCAATCATCAg                 >  2:2878943/1‑148 (MQ=255)
                                                                                                                            agGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCAGAGAATACCCAGCGCAATCAGTACTGCACCGCTGGCTGCAATCATCAg                 >  1:522959/1‑148 (MQ=255)
                                                                                                                             ggTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCAGAGAATACCCAGCGCAATCAGTACTGCACCGCTGGCTGCAATCATCAGc                <  1:1668843/148‑1 (MQ=255)
                                                                                                                                 gCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCAGAGAATACCCAGCGCAATCAGTACTGCACCGCTGGCTGCAATCATCAGCATgg            <  2:110804/148‑1 (MQ=255)
                                                                                                                                  cccACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCAGAGAATACCCAGCGCAATCAGTACTGCACCGCTGGCTGCAATCATCAGCATGgt           <  1:476094/148‑1 (MQ=255)
                                                                                                                                    cACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCAGAGAATACCCAGCGCAATCAGTACTGCACCGCTGGCTGCAATCATCAGCATGgtgt         <  2:284497/148‑1 (MQ=255)
                                                                                                                                     aCTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCAGAGAATACCCAGCGCAATCAGTACTGCACCGCTGGCTGCAATCATCAGCATGGtgtg        >  1:983857/1‑148 (MQ=255)
                                                                                                                                      cTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCAGAGAATACCCAGCGCAATCAGTACTGCACCGCTGGCTGCAATCATCAGCATGGTGTgg       >  1:1679702/1‑148 (MQ=255)
                                                                                                                                      cTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCAGAGAATACCCAGCGCAATCAGTACTGCACCGCTGGCTGCAATCATCAGCATGGTGTgg       <  1:2026731/148‑1 (MQ=255)
                                                                                                                                      cTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCAGAGAATACCCAGCGCAATCAGTACTGCACCGCTGGCTGCAATCATCAGCATGGTGTgg       <  1:3320964/148‑1 (MQ=255)
                                                                                                                                       tCCAGCGTACGGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCAGAGAATACCCAGCGCAATCAGTACTGCACCGCTGGCTGCAATCATCAGCATGGTGTGGa      >  2:780314/1‑148 (MQ=255)
                                                                                                                                       tCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCAGAGAATACCCAGCGCAATCAGTACTGCACCGCTGGCTGCAATCATCAGCATGGTGTGGa      >  2:634811/1‑148 (MQ=255)
                                                                                                                                        ccAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCAGAGAATACCCAGCGCAATCAGTACTGCACCGCTGGCTGCAATCATCAGCATGGTGTGGaa     <  2:2601868/148‑1 (MQ=255)
                                                                                                                                        ccAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCAGAGAATACCCAGCGCAATCAGTACTGCACCGCTGGCTGCAATCATCAGCATGGTGTGGaa     <  2:2368273/148‑1 (MQ=255)
                                                                                                                                           gCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCAGAGAATACCCAGCGCAATCAGTACTGCACCGCTGGCTGCAATCATCAGCATGGTGTGGAACTg  <  2:3118195/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                             
ATATGAATTTCTTCATAGTGGTCAGGCTTTTTATACGCTTCGCCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACGGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCAGAGAATACCCAGCGCAATCAGTACTGCACCGCTGGCTGCAATCATCAGCATGGTGTGGAACTG  >  CP009273/444344‑444630

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CGGCATATGAATTTCTTCATAGTGGTCAGGCTTTTTATACGCTTCGCCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACGGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCAGAGAATACCCAGCGCAATCAGTACTGCACCGCTGGCTGCAATCATCAGCATGGTGTGGAACTGCGGGTCA  >  CP009273/444340‑444637
                                                                                                                                                     |                                                                                                                                                    
CGGCATATGAATTTCTTCATAGTGGTCAGGCTTTTTATACGCTTCGCCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACA                                                                                                                                                      <  SRR19973065.2014286/150‑1 (MQ=60)
    ATATGAATTTCTTCATAGTGGTCAGGCTTTTTATACGCTTCGCCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCA                                                                                                                                                  >  SRR19973065.1454050/1‑150 (MQ=60)
     TATGAATTTCTTCATAGTGGTCAGGCTTTTTATACGCTTCGCCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCAC                                                                                                                                                 >  SRR19973065.2237999/1‑150 (MQ=60)
      ATGAATTTCTTCATAGTGGTCAGGCTTTTTATACGCTTCGCCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACC                                                                                                                                                >  SRR19973065.2206167/1‑150 (MQ=60)
       TGAATTTCTTCATAGTGGTCAGGCTTTTTATACGCTTCGCCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCC                                                                                                                                               >  SRR19973065.3471366/1‑150 (MQ=60)
        GAATTTCTTCATAGTGGTCAGGCTTTTTATACGCTTCGCCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCC                                                                                                                                              >  SRR19973065.739267/1‑150 (MQ=60)
        GAATTTCTTCATAGTGGTCAGGCTTTTTATACGCTTCGCCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCC                                                                                                                                              >  SRR19973065.739283/1‑150 (MQ=60)
        GAATTTCTTCATAGTGGTCAGGCTTTTTATACGCTTCGCCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCC                                                                                                                                              >  SRR19973065.739312/1‑150 (MQ=60)
          ATTTCTTCATAGTGGTCAGGCTTTTTATACGCTTCGCCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCAC                                                                                                                                            >  SRR19973065.3193902/1‑150 (MQ=60)
          ATTTCTTCATAGTGGTCAGGCTTTTTATACGCTTCGCCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCAC                                                                                                                                            >  SRR19973065.221216/1‑150 (MQ=60)
            TTCTTCATAGTGGTCAGGCTTTTTATACGCTTCGCCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGG                                                                                                                                          <  SRR19973065.1603470/150‑1 (MQ=60)
            TTCTTCATAGTGGTCAGGCTTTTTATACGCTTCGCCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGG                                                                                                                                          <  SRR19973065.1602814/150‑1 (MQ=60)
                    AGTGGTCAGGCTTTTTATACGCTTCGCCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAG                                                                                                                                  >  SRR19973065.143401/1‑150 (MQ=60)
                    AGTGGTCAGGCTTTTTATACGCTTCGCCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAG                                                                                                                                  >  SRR19973065.2265715/1‑150 (MQ=60)
                      TGGTCAGGCTTTTTATACGCTTCGCCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTC                                                                                                                                <  SRR19973065.2721813/150‑1 (MQ=60)
                      TGGTCAGGCTTTTTATACGCTTCGCCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTC                                                                                                                                <  SRR19973065.1537547/150‑1 (MQ=60)
                        GTCAGGCTTTTTATACGCTTCGCCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAG                                                                                                                              >  SRR19973065.592009/1‑150 (MQ=60)
                           AGGCTTTTTATACGCTTCGCCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTC                                                                                                                           >  SRR19973065.965877/1‑150 (MQ=60)
                           AGGCTTTTTATACGCTTCGCCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTC                                                                                                                           <  SRR19973065.201347/150‑1 (MQ=60)
                             GCTTTTTATACGCTTCGCCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCAC                                                                                                                         >  SRR19973065.1635930/1‑150 (MQ=60)
                              CTTTTTATACGCTTCGCCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACG                                                                                                                        >  SRR19973065.532345/1‑150 (MQ=60)
                               TTTTTATACGCTTCGCCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGG                                                                                                                       >  SRR19973065.1197855/1‑150 (MQ=60)
                                TTTTATACGCTTCGCCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGT                                                                                                                      <  SRR19973065.3030733/150‑1 (MQ=60)
                                TTTTATACGCTTCGCCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGT                                                                                                                      >  SRR19973065.575704/1‑150 (MQ=60)
                                TTTTATACGCTTCGCCTTTCTCTTTCATGTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGT                                                                                                                      <  SRR19973065.3067585/150‑1 (MQ=60)
                                  TTATACGCTTCGCCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTC                                                                                                                    >  SRR19973065.2720926/1‑150 (MQ=60)
                                   TATACGCTTCGCCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCT                                                                                                                   >  SRR19973065.2901895/1‑150 (MQ=60)
                                    ATACGCTTCGCCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTG                                                                                                                  >  SRR19973065.2383036/1‑150 (MQ=60)
                                    ATACGCTTCGCCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTG                                                                                                                  >  SRR19973065.2383608/1‑150 (MQ=60)
                                       CGCTTCGCCTTTCTCTTTCATTTCCCAGAATGCATCACTTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTC                                                                                                               <  SRR19973065.2236974/150‑1 (MQ=60)
                                       CGCTTCGCCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTC                                                                                                               >  SRR19973065.2532439/1‑150 (MQ=60)
                                        GCTTCGCCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCG                                                                                                              >  SRR19973065.1207862/1‑150 (MQ=60)
                                        GCTTCGCCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCG                                                                                                              <  SRR19973065.1657487/150‑1 (MQ=60)
                                        GCTTCGCCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCG                                                                                                              >  SRR19973065.1207881/1‑150 (MQ=60)
                                        GCTTCGCCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCG                                                                                                              >  SRR19973065.73547/1‑150 (MQ=60)
                                         CTTCGCCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGC                                                                                                             >  SRR19973065.1646864/1‑150 (MQ=60)
                                         CTTCGCCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGC                                                                                                             >  SRR19973065.1983507/1‑150 (MQ=60)
                                         CTTCGCCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGC                                                                                                             >  SRR19973065.862335/1‑150 (MQ=60)
                                         CTTCGCCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGC                                                                                                             >  SRR19973065.862250/1‑150 (MQ=60)
                                         CTTCGCCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGC                                                                                                             >  SRR19973065.1646907/1‑150 (MQ=60)
                                           TCGCCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGG                                                                                                           >  SRR19973065.1156138/1‑150 (MQ=60)
                                              CCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCG                                                                                                        <  SRR19973065.472268/150‑1 (MQ=60)
                                              CCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCG                                                                                                        <  SRR19973065.1772119/150‑1 (MQ=60)
                                              CCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCG                                                                                                        <  SRR19973065.2567749/150‑1 (MQ=60)
                                              CCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCG                                                                                                        <  SRR19973065.2567762/150‑1 (MQ=60)
                                              CCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCG                                                                                                        >  SRR19973065.180755/1‑150 (MQ=60)
                                                TTTCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCG                                                                                                      >  SRR19973065.219543/1‑150 (MQ=60)
                                                   CTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAAT                                                                                                   <  SRR19973065.858735/150‑1 (MQ=60)
                                                     CTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAG                                                                                                 <  SRR19973065.885237/150‑1 (MQ=60)
                                                     CTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAG                                                                                                 <  SRR19973065.1191228/150‑1 (MQ=60)
                                                     CTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAG                                                                                                 <  SRR19973065.1191190/150‑1 (MQ=60)
                                                      TTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGA                                                                                                >  SRR19973065.2601169/1‑150 (MQ=60)
                                                      TTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGA                                                                                                <  SRR19973065.3343508/150‑1 (MQ=60)
                                                      TTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGA                                                                                                <  SRR19973065.3343587/150‑1 (MQ=60)
                                                         CATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAAC                                                                                             <  SRR19973065.1009932/150‑1 (MQ=60)
                                                         CATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAAC                                                                                             <  SRR19973065.143144/150‑1 (MQ=60)
                                                         CATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAAC                                                                                             <  SRR19973065.1787107/150‑1 (MQ=60)
                                                            TTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTA                                                                                          >  SRR19973065.49324/1‑150 (MQ=60)
                                                            TTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTA                                                                                          >  SRR19973065.49318/1‑150 (MQ=60)
                                                              CCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACA                                                                                        <  SRR19973065.3487553/150‑1 (MQ=60)
                                                               CCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACAT                                                                                       <  SRR19973065.574268/150‑1 (MQ=60)
                                                                CAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGACGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATC                                                                                      >  SRR19973065.821439/1‑150 (MQ=60)
                                                                 AGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCT                                                                                     >  SRR19973065.792411/1‑150 (MQ=60)
                                                                 AGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCT                                                                                     >  SRR19973065.792403/1‑150 (MQ=60)
                                                                  GAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTG                                                                                    <  SRR19973065.297107/150‑1 (MQ=60)
                                                                  GAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTG                                                                                    <  SRR19973065.2805214/150‑1 (MQ=60)
                                                                  GAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTG                                                                                    <  SRR19973065.1771837/150‑1 (MQ=60)
                                                                   AATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGA                                                                                   >  SRR19973065.2596076/1‑150 (MQ=60)
                                                                   AATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGA                                                                                   >  SRR19973065.2595543/1‑150 (MQ=60)
                                                                     TGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAAT                                                                                 <  SRR19973065.510895/150‑1 (MQ=60)
                                                                     TGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAAT                                                                                 <  SRR19973065.510854/150‑1 (MQ=60)
                                                                        ATCACGTTCGTGAACGTGTGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAAC                                                                              <  SRR19973065.2724453/150‑1 (MQ=60)
                                                                        ATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATCGAAACGTACATCTGAATAAC                                                                              >  SRR19973065.967476/1‑150 (MQ=60)
                                                                        ATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAAC                                                                              >  SRR19973065.2533624/1‑150 (MQ=60)
                                                                        ATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAAC                                                                              >  SRR19973065.836200/1‑150 (MQ=60)
                                                                        ATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAAC                                                                              <  SRR19973065.1534394/150‑1 (MQ=60)
                                                                        ATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAAC                                                                              >  SRR19973065.762006/1‑150 (MQ=60)
                                                                        ATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAAC                                                                              >  SRR19973065.1214881/1‑150 (MQ=60)
                                                                        ATCACGTTCGTGAACGTGCGGCACTACGGAAAAGTTATAGAACGGAGTCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACTGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAAAGTACATCTGAATAAC                                                                              >  SRR19973065.1214215/1‑150 (MQ=60)
                                                                         TCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACG                                                                             <  SRR19973065.953522/150‑1 (MQ=60)
                                                                             GTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGC                                                                         >  SRR19973065.1895519/1‑150 (MQ=60)
                                                                             GTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGC                                                                         >  SRR19973065.1895502/1‑150 (MQ=60)
                                                                              TTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCA                                                                        <  SRR19973065.326777/150‑1 (MQ=60)
                                                                              TTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCA                                                                        <  SRR19973065.1858194/150‑1 (MQ=60)
                                                                               TCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCAG                                                                       <  SRR19973065.554550/150‑1 (MQ=60)
                                                                                CGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCAGA                                                                      <  SRR19973065.1047854/150‑1 (MQ=60)
                                                                                     ACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCAGAGAATA                                                                 <  SRR19973065.1401466/150‑1 (MQ=60)
                                                                                       GTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCAGAGAATACC                                                               <  SRR19973065.1464650/150‑1 (MQ=60)
                                                                                        TGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCAGAGAATACCC                                                              >  SRR19973065.2453425/1‑150 (MQ=60)
                                                                                         GCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCAGAGAATACCCA                                                             <  SRR19973065.2420278/150‑1 (MQ=60)
                                                                                          CGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCAGAGAATACCCAG                                                            <  SRR19973065.2932418/150‑1 (MQ=60)
                                                                                           GGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCAGAGAATACCCAGC                                                           <  SRR19973065.3325749/150‑1 (MQ=60)
                                                                                            GCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCAGAGAATACCCAGCG                                                          <  SRR19973065.847645/150‑1 (MQ=60)
                                                                                                 ACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCAGAGAATACCCAGCGCAATC                                                     <  SRR19973065.2141389/150‑1 (MQ=60)
                                                                                                   GGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCAGAGAATACCCAGCGCAATCAG                                                   >  SRR19973065.851360/1‑150 (MQ=60)
                                                                                                    GCAAAGTTATAGAACGGAGGCGGGTAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTAGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCAGAGAATACCCAGCGCAATCTGT                                                  <  SRR19973065.1688135/150‑1 (MQ=60)
                                                                                                     CAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCAGAGAATACCCAGCGCAATCAGTA                                                 >  SRR19973065.908509/1‑150 (MQ=60)
                                                                                                       AAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCAGAGAATACCCAGCGCAATCAGTACT                                               <  SRR19973065.2612780/150‑1 (MQ=60)
                                                                                                       AAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCAGAGAATACCCAGCGCAATCAGTACT                                               <  SRR19973065.2179873/150‑1 (MQ=60)
                                                                                                       AAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCAGAGAATACCCAGCGCAATCAGTACT                                               >  SRR19973065.1552587/1‑150 (MQ=60)
                                                                                                       AAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCAGAGAATACCCAGCGCAATCAGTACT                                               >  SRR19973065.1552585/1‑150 (MQ=60)
                                                                                                        AGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCAGAGAATACCCAGCGCAATCAGTACTG                                              >  SRR19973065.283470/1‑150 (MQ=60)
                                                                                                           TATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCAGAGAATACCCAGCGCAATCAGTACTGCAC                                           <  SRR19973065.2967621/150‑1 (MQ=60)
                                                                                                              AGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCAGAGAATACCCAGCGCAATCAGTACTGCACCGC                                        >  SRR19973065.1537056/1‑150 (MQ=60)
                                                                                                              AGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCAGAGAATACCCAGCGCAATCAGTACTGCACCGC                                        >  SRR19973065.3372044/1‑150 (MQ=60)
                                                                                                              AGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCAGAGAATACCCAGCGCAATCAGTACTGCACCGC                                        >  SRR19973065.3371999/1‑150 (MQ=60)
                                                                                                                AACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCAGAGAATACCCAGCGCAATCAGTACTGCACCGCTG                                      <  SRR19973065.2910151/150‑1 (MQ=60)
                                                                                                                AACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCAGAGAATACCCAGCGCAATCAGTACTGCACCGCTG                                      <  SRR19973065.760960/150‑1 (MQ=60)
                                                                                                                 ACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCAGAGAATACCCAGCGCAATCAGTACTGCACCGCTGG                                     <  SRR19973065.2969967/150‑1 (MQ=60)
                                                                                                                  CGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCAGAGAATACCCAGCGCAATCAGTACTGCACCGCTGGC                                    <  SRR19973065.2604508/150‑1 (MQ=60)
                                                                                                                   GGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCAGAGAATACCCAGCGCAATCAGTACTGCACCGCTGGCT                                   <  SRR19973065.1587470/150‑1 (MQ=60)
                                                                                                                      GGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCAGAGAATACCCAGCGCAATCAGTACTGCACCGCTGGCTGCA                                <  SRR19973065.2556685/150‑1 (MQ=60)
                                                                                                                            GAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCAGAGAATACCCAGCGCAATCAGTACTGCACCGCTGGCTGCAATCATC                          <  SRR19973065.2098064/150‑1 (MQ=60)
                                                                                                                            GAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCAGAGAATACCCAGCGCAATCAGTACTGCACCGCTGGCTGCAATCATC                          <  SRR19973065.2098125/150‑1 (MQ=60)
                                                                                                                             AAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCAGAGAATACCCAGCGCAATCAGTACTGCACCGCTGGCTGCAATCATCA                         <  SRR19973065.1901839/150‑1 (MQ=60)
                                                                                                                             AAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCAGAGAATACCCAGCGCAATCAGTACTGCACCGCTGGCTGCAATCATCA                         >  SRR19973065.2467785/1‑150 (MQ=60)
                                                                                                                              AGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCAGAGAATACCCAGCGCAATCAGTACTGCACCGCTGGCTGCAATCATCAG                        >  SRR19973065.526399/1‑150 (MQ=60)
                                                                                                                                 GGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCAGAGAATACCCAGCGCAATCAGTACTGCACCGCTGGCTGCAATCATCAGCAT                     <  SRR19973065.1209444/150‑1 (MQ=60)
                                                                                                                                 GGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCAGAGAATACCCAGCGCAATCAGTACTGCACCGCTGGCTGCAATCATCAGCAT                     <  SRR19973065.1680009/150‑1 (MQ=60)
                                                                                                                                     GCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCAGAGAATACCCAGCGCAATCAGTACTGCACCGCTGGCTGCACTCATCAGCATGGTG                 >  SRR19973065.2803690/1‑150 (MQ=60)
                                                                                                                                      CCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCAGAGAATACCCAGCGCAATCAGTACTGCACCGCTGGCTGCAATCATCAGCATGGTGT                <  SRR19973065.479340/150‑1 (MQ=60)
                                                                                                                                       CCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCAGAGAATACCCAGCGCAATCAGTACTGCACCGCTGGCTGCAATCATCAGCATGGTGTG               >  SRR19973065.990392/1‑150 (MQ=60)
                                                                                                                                        CACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCAGAGAATACCCAGCGCAATCAGTACTGCACCGCTGGCTGCAATCATCAGCATGGTGTGG              >  SRR19973065.2185772/1‑150 (MQ=60)
                                                                                                                                        CACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCAGAGAATACCCAGCGCAATCAGTACTGCACCGCTGGCTGCAATCATCAGCATGGTGTGG              >  SRR19973065.1690931/1‑150 (MQ=60)
                                                                                                                                        CACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCAGAGAATACCCAGCGCAATCAGTACTGCACCGCTGGCTGCAATCATCAGCATGGTGTGG              >  SRR19973065.1691917/1‑150 (MQ=60)
                                                                                                                                          CTCCAGCGTACAGCCAGCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCAGAGAATACCCAGCGCAATCAGTACTGCACCGCTGGCTGCAATCATCAGCATGGTGTGGAA            <  SRR19973065.2039770/150‑1 (MQ=60)
                                                                                                                                          CTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCAGAGAATACCCAGCGCAATCAGTACTGCACCGCTGGCTGCAATCATCAGCATGGTGTGGAA            <  SRR19973065.2039763/150‑1 (MQ=60)
                                                                                                                                          CTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCAGAGAATACCCAGCGCAATCAGTACTGCACCGCTGGCTGCAATCATCAGCATGGTGTGGAA            <  SRR19973065.3341223/150‑1 (MQ=60)
                                                                                                                                          CTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCAGAGAATACCCAGCGCAATCAGTACTGCACCGCTGGCTGCAATCATCAGCATGGTGTGGAA            >  SRR19973065.1772461/1‑150 (MQ=60)
                                                                                                                                          CTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCAGAGAATACCCAGCGCAATCAGTACTGCACCGCTGGCTGCAATCATCAGCATGGTGTGGAA            <  SRR19973065.2039755/150‑1 (MQ=60)
                                                                                                                                                    CAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCAGAGAATACCCAGCGCAATCAGTACTGCACCGCTGGCTGCAATCATCAGCATGGTGTGGAACTGCGGGTCA  <  SRR19973065.2082315/150‑1 (MQ=60)
                                                                                                                                                     |                                                                                                                                                    
CGGCATATGAATTTCTTCATAGTGGTCAGGCTTTTTATACGCTTCGCCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACGGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCAGAGAATACCCAGCGCAATCAGTACTGCACCGCTGGCTGCAATCATCAGCATGGTGTGGAACTGCGGGTCA  >  CP009273/444340‑444637

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑