Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F434 I0 R1 1130 215.9 7031662 97.1% 6827743 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 551,499 G→T 100% R171S (CGC→AGC)  ybcI ← DUF457 family inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273551,4990GT99.5% 626.6 / ‑4.3 191R171S (CGC→AGC) ybcIDUF457 family inner membrane protein
Reads supporting (aligned to +/- strand):  ref base G (0/1);  new base T (104/86);  total (104/87)
Fisher's exact test for biased strand distribution p-value = 4.55e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 191 total aligned reads displayed.

AAGACTGGGCGGCGGCGGATGCGGCGCGTGATCGTCTTAACGAGATGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCAAACGGCGCGACTTTTATCACCTGCCAGGGAGCGAAAAAGC  >  CP009273/551355‑551642
                                                                                                                                                |                                                                                                                                               
 aGACTGGGCGGCGGCGGATGCGGCGCGTGATCGTCTTAACGAGATGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCTccac                                                                                                                                             >  2:1649345/1‑148 (MQ=255)
 aGACTGGGCGGCGGCGGATGCGGCGCGTGATCGTCTTAACGAGATGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCTccac                                                                                                                                             >  2:378170/1‑148 (MQ=255)
  gACTGGGCGGCGGCGGATGCGGCGCGTGATCGTCTTAACGAGATGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCTccacc                                                                                                                                            >  2:3126203/1‑148 (MQ=255)
     tGGGCGGCGGCGGATGCGGCGCGTGATCGTCTTAACGAGATGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCTCCACcaca                                                                                                                                         >  1:1185107/1‑148 (MQ=255)
     tGGGCGGCGGCGGATGCGGCGCGTGATCGTCTTAACGAGATGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCTCCACcaca                                                                                                                                         >  1:2634791/1‑148 (MQ=255)
       ggcggcggcGGATGCGGCGCGTGATCGTCTTAACGAGATGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCTCCACCacaac                                                                                                                                       >  1:2484551/1‑148 (MQ=255)
       ggcggcggcGGATGCGGCGCGTGATCGTCTTAACGAGATGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCTCCACCacaac                                                                                                                                       >  1:1547862/1‑148 (MQ=255)
         cggcggcggATGCGGCGCGTGATCGTCTTAACGAGATGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCTCCACCACAACAt                                                                                                                                     <  2:3167835/148‑1 (MQ=255)
         cggcggcggATGCGGCGCGTGATCGTCTTAACGAGATGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCTCCACCACAACAt                                                                                                                                     <  2:2271950/148‑1 (MQ=255)
         cggcggcggATGCGGCGCGTGATCGTCTTAACGAGATGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCTCCACCACAACAt                                                                                                                                     >  1:810796/1‑148 (MQ=255)
           gcggcggATGCGGCGCGTGATCGTCTTAACGAGATGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCTCCACCACAACATTc                                                                                                                                   <  1:333116/148‑1 (MQ=255)
           gcggcggATGCGGCGCGTGATCGTCTTAACGAGATGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCTCCACCACAACATTc                                                                                                                                   <  1:203508/148‑1 (MQ=255)
            cggcggATGCGGCGCGTGATCGTCTTAACGAGATGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCTCCACCACAACATTcc                                                                                                                                  <  2:2681324/148‑1 (MQ=255)
              gcggATGCGGCGCGTGATCGTCTTAACGAGATGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCTCCACCACAACATTCCCa                                                                                                                                <  2:1163686/148‑1 (MQ=255)
               cggATGCGGCGCGTGATCGTCTTAACGAGATGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCTCCACCACAACATTCCCAt                                                                                                                               <  1:3385817/148‑1 (MQ=255)
               cggATGCGGCGCGTGATCGTCTTAACGAGATGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCTCCACCACAACATTCCCAt                                                                                                                               <  1:822967/148‑1 (MQ=255)
               cggATGCGGCGCGTGATCGTCTTAACGAGATGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCTCCACCACAACATTCCCAt                                                                                                                               <  2:90896/148‑1 (MQ=255)
                ggATGCGGCGCGTGATCGTCTTAACGAGATGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCTCCACCACAACATTCCCATc                                                                                                                              <  1:2680456/148‑1 (MQ=255)
                        cgcgTGATCGTCTTAACGAGATGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCTCCACCACAACATTCCCATCAGTAGCAt                                                                                                                      <  2:240449/148‑1 (MQ=255)
                         gcgTGATCGTCTTAACGAGATGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCTCCACCACAACATTCCCATCAGTAGCATc                                                                                                                     <  1:762604/148‑1 (MQ=255)
                            tGATCGTCTTAACGAGATGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCTCCACCACAACATTCCCATCAGTAGCATcccc                                                                                                                  <  2:2118762/148‑1 (MQ=255)
                                  tCTTAACGAGATGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCTCCACCACAACATTCCCATCAGTAGCATCCCCGGCAAc                                                                                                            >  1:1219808/1‑148 (MQ=255)
                                  tCTTAACGAGATGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCTCCACCACAACATTCCCATCAGTAGCATCCCCGGCAAc                                                                                                            >  2:3305714/1‑148 (MQ=255)
                                   cTTAACGAGATGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCTCCACCACAACATTCCCAGCAGTAGCATCCCCGGCAAcc                                                                                                           >  2:1022503/1‑148 (MQ=255)
                                    ttAACGAGATGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCTCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACca                                                                                                          <  1:860135/148‑1 (MQ=255)
                                     tAACGAGATGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCTCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACcac                                                                                                         >  1:529337/1‑148 (MQ=255)
                                     tAACGAGATGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCTCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACcac                                                                                                         >  1:3124436/1‑148 (MQ=255)
                                       aCGAGATGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCTCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCacac                                                                                                       >  1:1985875/1‑148 (MQ=255)
                                       aCGAGATGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCTCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCacac                                                                                                       >  2:460927/1‑148 (MQ=255)
                                        cGAGATGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCTCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACAcc                                                                                                      >  2:2816868/1‑148 (MQ=255)
                                         gagaTGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCTCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACAccc                                                                                                     <  1:2268585/148‑1 (MQ=255)
                                               gggATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCTCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCa                                                                                               >  2:2507849/1‑148 (MQ=255)
                                                ggATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCTCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCaa                                                                                              >  1:1493800/1‑148 (MQ=255)
                                                 gATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCTCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAAt                                                                                             >  2:1399285/1‑148 (MQ=255)
                                                          ggAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCTCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAaata                                                                                    <  1:768211/148‑1 (MQ=255)
                                                          ggAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCTCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACAGCAATTCAGAaata                                                                                    >  2:755450/1‑148 (MQ=255)
                                                            aaGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCTCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAaataat                                                                                  >  2:2003165/1‑148 (MQ=255)
                                                            aaGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCTCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAaataat                                                                                  >  1:2603898/1‑148 (MQ=255)
                                                             aGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCTCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATc                                                                                 <  2:1479850/148‑1 (MQ=255)
                                                             aGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCTCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATc                                                                                 >  1:278466/1‑148 (MQ=255)
                                                              gATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCTCTATTGCCTGATGCGAGTTTTCTCATCCGGTTATCGTCTGCTCCACCACAACATTCCCATCAGTAGCATCCCAGGCAACCACACCCACATCAATTCAGAAATAATCa                                                                                <  2:2933478/148‑1 (MQ=255)
                                                               aTGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCTCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCAc                                                                               >  2:1449804/1‑148 (MQ=255)
                                                                  gCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCTCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGACATAATCACCTg                                                                            >  2:1275194/1‑148 (MQ=255)
                                                                  gCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCTCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTg                                                                            >  2:916183/1‑148 (MQ=255)
                                                                   cccGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCTCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGa                                                                           >  2:522140/1‑148 (MQ=255)
                                                                       cAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCTCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGcc                                                                       >  1:103025/1‑148 (MQ=255)
                                                                       cAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCTCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGcc                                                                       >  1:103091/1‑148 (MQ=255)
                                                                       cAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCTCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGcc                                                                       <  2:2603898/148‑1 (MQ=255)
                                                                         aGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCTCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCg                                                                     >  2:1407670/1‑148 (MQ=255)
                                                                              ccaccTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCTCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACgg                                                                >  1:1568408/1‑148 (MQ=255)
                                                                                accTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCTCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCg                                                              >  1:3234642/1‑148 (MQ=255)
                                                                                accTGGCGTCGTAAGTAATTGCGCTAGTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCTCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCg                                                              <  2:3299338/148‑1 (MQ=255)
                                                                                   tGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCTCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCgtgg                                                           <  1:1290548/148‑1 (MQ=255)
                                                                                    ggCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCTCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCgtggt                                                          <  1:544674/148‑1 (MQ=255)
                                                                                     gCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCTCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCgtggtg                                                         <  1:1896037/148‑1 (MQ=255)
                                                                                          gtaagtaaTTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCTCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACg                                                    <  2:174735/148‑1 (MQ=255)
                                                                                           taagtaaTTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCTCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACga                                                   >  2:819754/1‑148 (MQ=255)
                                                                                            aagtaaTTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCTCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACgag                                                  >  1:2162253/1‑148 (MQ=255)
                                                                                            aagtaaTTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCTCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACgag                                                  >  1:785237/1‑148 (MQ=255)
                                                                                             agtaaTTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCTCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACgaga                                                 <  1:1645491/148‑1 (MQ=255)
                                                                                             agtaaTTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCTCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACgaga                                                 <  2:2021737/148‑1 (MQ=255)
                                                                                               taaTTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCTCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTCACGAGACa                                               >  2:3093708/1‑148 (MQ=255)
                                                                                                  ttGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCTCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATg                                            >  1:304028/1‑148 (MQ=255)
                                                                                                     cgcTATTGCCGGATGCGAGTTTTCGCATCCGGTTTTCGTCTGCTCCACCACAACCTTCCCATCAGTAGCATCCCCGGCAACCACACCCTCATCAATTCAGAAATAATCACCTGATGCCCGTACGGCTTGGTGTAACGATACAATGcta                                         <  2:1189991/148‑3 (MQ=255)
                                                                                                     cgcTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCTCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGcta                                         <  2:1189874/148‑3 (MQ=255)
                                                                                                         aTTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCTCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCTAACgg                                     >  1:703634/1‑148 (MQ=255)
                                                                                                         aTTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCTCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCTAACgg                                     <  1:3397895/148‑1 (MQ=255)
                                                                                                           tGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCTCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCTAACGgcg                                   >  2:1239526/1‑148 (MQ=255)
                                                                                                              cGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCTCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCTAACGGCGCGa                                <  1:460927/148‑1 (MQ=255)
                                                                                                                 aTGCGAGTTTTCGCATCCGGTTATCGTCTGCTCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCTAACGGCGCGACtt                             <  2:29843/148‑1 (MQ=255)
                                                                                                                   gCGAGTTTTCGCATCCGGTTATCGTCTGCTCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCTAACGGCGCGACtttt                           <  1:1467600/148‑1 (MQ=255)
                                                                                                                   gCGAGTTTTCGCATCCGGTTATCGTCTGCTCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCTAACGGCGCGACtttt                           <  2:1219808/148‑1 (MQ=255)
                                                                                                                   gCGAGTTTTCGCATCCGGTTATCGTCTGCTCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCTAACGGCGCGACtttt                           >  2:2512719/1‑148 (MQ=255)
                                                                                                                    cGAGTTTTCGCATCCGGTTATCGTCTGCTCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCTAACGGCGCGACTTTTa                          >  2:662866/1‑148 (MQ=255)
                                                                                                                    cGAGTTTTCGCATCCGGTTATCGTCTGCTCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCTAACGGCGCGACTTTTa                          <  1:3126203/148‑1 (MQ=255)
                                                                                                                     gAGTTTTCGCATCCGGTTATCGTCTGCTCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCTAACGGCGCGACTTTTAt                         <  1:3354131/148‑1 (MQ=255)
                                                                                                                      aGTTTTCGCATCCGGTTATCGTCTGCTCCACCACAACATTCCCATCATTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGGGGTGTAACGAGACAATGCTAACGGCGCGACTTTTATc                        >  1:2883982/1‑148 (MQ=255)
                                                                                                                      aGTTTTCGCATCCGGTTATCGTCTGCTCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGACATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCAAACGGCGCGACTTTTATc                        >  2:3088722/1‑148 (MQ=255)
                                                                                                                       gTTTTCGCATCCGGTTATCGTCTGCTCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCTAACGGCGCGACTTTTATCa                       <  1:192898/148‑1 (MQ=255)
                                                                                                                           tCGCATCCGGTTATCGTCTGCTCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCTAACGGCGCGACTTTTATCACCTg                   >  1:2188736/1‑148 (MQ=255)
                                                                                                                              cATCCGGTTATCGTCTGCTCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCTAACGGCGCGACTTTTATCACCTGCCa                <  1:675872/148‑1 (MQ=255)
                                                                                                                               aTCCGGTTATCGTCTGCTCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCTAACGGCGCGACTTTTATCACCTGCCAg               >  2:1690412/1‑148 (MQ=255)
                                                                                                                                 ccGGTTATCGTCTGCTCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCTAACGGCGCGACTTTTATCACCTGCCAggg             >  1:1690367/1‑148 (MQ=255)
                                                                                                                                 ccGGTTATCGTCTGCTCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCTAACGGCGCGACTTTTATCACCTGCCAggg             >  1:1110474/1‑148 (MQ=255)
                                                                                                                                 ccGGTTATCGTCTGCTCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCTAACGGCGCGACTTTTATCACCTGCCAggg             <  1:284253/148‑1 (MQ=255)
                                                                                                                                 ccGGTTATCGTCTGCTCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCTAACGGCGCGACTTTTATCACCTGCCAggg             <  1:284251/148‑1 (MQ=255)
                                                                                                                                 ccGGTTATCGTCTGCTCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCTAACGGCGCGACTTTTATCACCTGCCAggg             >  1:1689546/1‑148 (MQ=255)
                                                                                                                                 ccGGTTATCGTCTGCTCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCTAACGGCGCGACTTTTATCACCTGCCAggg             <  1:2086644/148‑1 (MQ=255)
                                                                                                                                 ccGGTTATCGTCTGCTCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATAAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCTAACGGCGCGACTTTTATCACCTGCCAggg             <  1:608083/148‑1 (MQ=255)
                                                                                                                                     ttATCGTCTGCTCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCTAACGGCGCGACTTTTATCACCTGCCAGGGAGCg         >  2:2168929/1‑148 (MQ=255)
                                                                                                                                     ttATCGTCTGCTCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCTAACGGCGCGACTTTTATCACCTGCCAGGGAGCg         >  1:2536819/1‑148 (MQ=255)
                                                                                                                                       aTCGTCTGCTCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCTAACGGCGCGCCTTGTATCACCTGCCAGGGAGCGaa       >  2:2564278/1‑148 (MQ=255)
                                                                                                                                       aTCGTCTGCTCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCTAACGGCGCGACTTTTATCACCTGCCAGGGAGCGaa       >  2:221761/1‑148 (MQ=255)
                                                                                                                                       aTCGTCTGCTCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCTAACGGCGCGACTTTTATCACCTGCCAGGGAGCGaa       >  2:806098/1‑148 (MQ=255)
                                                                                                                                         cGTCTGCTCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCTAACGGCGCGACTTTTATCACCTGCCAGGGAGCGaaaa     >  2:1887687/1‑148 (MQ=255)
                                                                                                                                         cGTCTGCTCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCTAACGGCGCGACTTTTATCACCTGCCAGGGAGCGaaaa     <  2:3007735/148‑1 (MQ=255)
                                                                                                                                         cGTCTGCTCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCTAACGGCGCGACTTTTATCACCTGCCAGGGAGCGaaaa     <  2:1126986/148‑1 (MQ=255)
                                                                                                                                         cGTCTGCTCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCTAACGGCGCGACTTTTATCACCTGCCAGGGAGCGaaaa     <  1:819754/148‑1 (MQ=255)
                                                                                                                                           tCTGCTCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCTAACGGCGCGACTTTTATCACCTGCCAGGGAGCGAAAAAg   <  2:1060922/148‑1 (MQ=255)
                                                                                                                                            cTGCTCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCTAACGGCGCGACTTGTATCACCTGCCAGGGAGCGAAAAAGc  >  2:2796965/1‑148 (MQ=255)
                                                                                                                                                |                                                                                                                                               
AAGACTGGGCGGCGGCGGATGCGGCGCGTGATCGTCTTAACGAGATGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCAAACGGCGCGACTTTTATCACCTGCCAGGGAGCGAAAAAGC  >  CP009273/551355‑551642

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
AGCGAAAGACTGGGCGGCGGCGGATGCGGCGCGTGATCGTCTTAACGAGATGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCAAACGGCGCGACTTTTATCACCTGCCAGGGAGCGAAAAAGCGTTCAT  >  CP009273/551350‑551648
                                                                                                                                                     |                                                                                                                                                     
AGCGAAAGACTGGGCGGCGGCGGATGCGGCGCGTGATCGTCTTAACGAGATGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCT                                                                                                                                                       >  SRR19973065.1189700/1‑150 (MQ=60)
 GCGAAAGACTGGGCGGCGGCGGATGCGGCGCGTGATCGTCTTAACGAGATGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCTC                                                                                                                                                      <  SRR19973065.2522151/150‑1 (MQ=60)
 GCGAAAGACTGGGCGGCGGCGGATGCGGCGCGTGATCGTCTTAACGAGATGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCTC                                                                                                                                                      <  SRR19973065.2522838/150‑1 (MQ=60)
   GAAAGACTGGGCGGCGGCGGATGCGGCGCGTGATCGTCTTAACGAGATGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCTCCA                                                                                                                                                    <  SRR19973065.1591321/150‑1 (MQ=60)
        ACTGGGCGGCGGCGGATGCGGCGCGTGATCGTCTTAACGAGATGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCTCCACCACA                                                                                                                                               >  SRR19973065.1192607/1‑150 (MQ=60)
        ACTGGGCGGCGGCGGATGCGGCGCGTGATCGTCTTAACGAGATGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCTCCACCACA                                                                                                                                               >  SRR19973065.2651068/1‑150 (MQ=60)
          TGGGCGGCGGCGGATGCGGCGCGTGATCGTCTTAACGAGATGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCTCCACCACAAC                                                                                                                                             >  SRR19973065.2500326/1‑150 (MQ=60)
          TGGGCGGCGGCGGATGCGGCGCGTGATCGTCTTAACGAGATGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCTCCACCACAAC                                                                                                                                             >  SRR19973065.2500186/1‑150 (MQ=60)
          TGGGCGGCGGCGGATGCGGCGCGTGATCGTCTTAACGAGATGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCTCCACCACAAC                                                                                                                                             >  SRR19973065.1558104/1‑150 (MQ=60)
            GGCGGCGGCGGATGCGGCGCGTGATCGTCTTAACGAGATGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCTCCACCACAACAT                                                                                                                                           >  SRR19973065.816150/1‑150 (MQ=60)
            GGCGGCGGCGGATGCGGCGCGTGATCGTCTTAACGAGATGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCTCCACCACAACAT                                                                                                                                           >  SRR19973065.816144/1‑150 (MQ=60)
               GGCGGCGGATGCGGCGCGTGATCGTCTTAACGAGATGGGGATCGTGCTGGAAGATGGACCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCTCCACCACAACATTCC                                                                                                                                        <  SRR19973065.3378913/150‑1 (MQ=60)
                GCGGCGGATGCGGCGCGTGATCGTCTTAACGAGATGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCTCCACCACAACATTCCC                                                                                                                                       <  SRR19973065.335445/150‑1 (MQ=60)
                GCGGCGGATGCGGCGCGTGATCGTCTTAACGAGATGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCTCCACCACAACATTCCC                                                                                                                                       <  SRR19973065.205069/150‑1 (MQ=60)
                GCGGCGGATGCGGCGCGTGATCGTCTTAACGAGATGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCTCCACCACAACATTCCC                                                                                                                                       <  SRR19973065.205000/150‑1 (MQ=60)
                    CGGATGCGGCGCGTGATCGTCTTAACGAGATGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCTCCACCACAACATTCCCATCA                                                                                                                                   <  SRR19973065.828367/150‑1 (MQ=60)
                    CGGATGCGGCGCGTGATCGTCTTAACGAGATGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCTCCACCACAACATTCCCATCA                                                                                                                                   <  SRR19973065.3406522/150‑1 (MQ=60)
                     GGATGCGGCGCGTGATCGTCTTAACGAGATGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCTCCACCACAACATTCCCATCAG                                                                                                                                  <  SRR19973065.2697077/150‑1 (MQ=60)
                            GCGCGTGATCGTCTTAACGAGATGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCTCCACCACAACATTCCCATCAGTAGCATC                                                                                                                           <  SRR19973065.1365931/150‑1 (MQ=60)
                            GCGCGTGATCGTCTTAACGAGATGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCTCCACCACAACATTCCCATCAGTAGCATC                                                                                                                           <  SRR19973065.762785/150‑1 (MQ=60)
                              GCGTGATCGTCTTAACGAGATGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCTCCACCACAACATTCCCATCAGTAGCATCCC                                                                                                                         <  SRR19973065.767680/150‑1 (MQ=60)
                                     CGTCTTAACGAGATGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCTCCACCACAACATTCCCATCAGTAGCATCCCCGGCAAC                                                                                                                  >  SRR19973065.1227733/1‑150 (MQ=60)
                                        CTTAACGAGATGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCTCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCAC                                                                                                               >  SRR19973065.532804/1‑150 (MQ=60)
                                        CTTAACGAGATGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCTCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCAC                                                                                                               >  SRR19973065.3143462/1‑150 (MQ=60)
                                         TTAACGAGATGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCTCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACA                                                                                                              <  SRR19973065.865677/150‑1 (MQ=60)
                                          TAACGAGATGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCTCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACAC                                                                                                             >  SRR19973065.1998768/1‑150 (MQ=60)
                                          TAACGAGATGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCTCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACAC                                                                                                             <  SRR19973065.264054/150‑1 (MQ=60)
                                              GAGATGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCTCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCAC                                                                                                         <  SRR19973065.2282914/150‑1 (MQ=60)
                                                  TGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCTCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCA                                                                                                     <  SRR19973065.2071797/150‑1 (MQ=60)
                                                   GGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCTCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAA                                                                                                    >  SRR19973065.1503396/1‑150 (MQ=60)
                                                   GGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCTCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAA                                                                                                    >  SRR19973065.2243636/1‑150 (MQ=60)
                                                   GGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCTCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAA                                                                                                    >  SRR19973065.2180341/1‑150 (MQ=60)
                                                    GGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCTCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAAT                                                                                                   <  SRR19973065.505511/150‑1 (MQ=60)
                                                              TGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCTCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAA                                                                                         >  SRR19973065.3529042/1‑150 (MQ=60)
                                                               GGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCTCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAAT                                                                                        >  SRR19973065.2620065/1‑150 (MQ=60)
                                                               GGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCTCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAAT                                                                                        <  SRR19973065.773317/150‑1 (MQ=60)
                                                                GAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCTCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATC                                                                                       >  SRR19973065.280321/1‑150 (MQ=60)
                                                                GAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCTCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATC                                                                                       <  SRR19973065.2483920/150‑1 (MQ=60)
                                                                 AAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCTCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAAAAATCA                                                                                      >  SRR19973065.1945189/1‑150 (MQ=60)
                                                                          CGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCTCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCC                                                                             >  SRR19973065.103976/1‑150 (MQ=60)
                                                                          CGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCTCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCC                                                                             >  SRR19973065.104042/1‑150 (MQ=60)
                                                                                 GACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCTCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGG                                                                      >  SRR19973065.1578838/1‑150 (MQ=60)
                                                                                 GACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCTCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGG                                                                      >  SRR19973065.1175759/1‑150 (MQ=60)
                                                                                 GACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCTCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGG                                                                      >  SRR19973065.1578492/1‑150 (MQ=60)
                                                                                  ACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCTCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGC                                                                     >  SRR19973065.2999758/1‑150 (MQ=60)
                                                                                   CCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCTCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCG                                                                    >  SRR19973065.3254101/1‑150 (MQ=60)
                                                                                    CACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCTCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGT                                                                   >  SRR19973065.636779/1‑150 (MQ=60)
                                                                                        TGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCTCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTG                                                               <  SRR19973065.1916431/150‑1 (MQ=60)
                                                                                        TGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCTCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTG                                                               <  SRR19973065.1299056/150‑1 (MQ=60)
                                                                                        TGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCTCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTG                                                               <  SRR19973065.1299020/150‑1 (MQ=60)
                                                                                        TGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCTCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTG                                                               >  SRR19973065.1222840/1‑150 (MQ=60)
                                                                                         GGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCTCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGT                                                              <  SRR19973065.548177/150‑1 (MQ=60)
                                                                                         GGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCTCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGT                                                              <  SRR19973065.548197/150‑1 (MQ=60)
                                                                                         GGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCTCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGT                                                              <  SRR19973065.1685528/150‑1 (MQ=60)
                                                                                          GCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCTCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTA                                                             <  SRR19973065.1908502/150‑1 (MQ=60)
                                                                                            GTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCTCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAAC                                                           <  SRR19973065.89652/150‑1 (MQ=60)
                                                                                              CGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCTCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGA                                                         >  SRR19973065.163416/1‑150 (MQ=60)
                                                                                               GTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCTCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAG                                                        >  SRR19973065.2176150/1‑150 (MQ=60)
                                                                                               GTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCTCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAG                                                        >  SRR19973065.790417/1‑150 (MQ=60)
                                                                                               GTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCTCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAG                                                        >  SRR19973065.790448/1‑150 (MQ=60)
                                                                                                  AGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCTCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACA                                                     <  SRR19973065.1656537/150‑1 (MQ=60)
                                                                                                     AATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCTCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATG                                                  >  SRR19973065.306039/1‑150 (MQ=60)
                                                                                                          CGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCTCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCTAAC                                             <  SRR19973065.694208/150‑1 (MQ=60)
                                                                                                          CGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCTCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCTAAC                                             <  SRR19973065.152088/150‑1 (MQ=60)
                                                                                                          CGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCTCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCTAAC                                             <  SRR19973065.2721887/150‑1 (MQ=60)
                                                                                                            CTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCTCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCTAACGG                                           >  SRR19973065.708313/1‑150 (MQ=60)
                                                                                                              ATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCTCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCTAACGGCG                                         <  SRR19973065.3418669/150‑1 (MQ=60)
                                                                                                                   CGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCTCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCTAACGGCGCGACT                                    <  SRR19973065.464105/150‑1 (MQ=60)
                                                                                                                      ATGCGAGTTTTCGCATCCGGTTATCGTCTGCTCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCTAACGGCGCGACTTTT                                 >  SRR19973065.825144/1‑150 (MQ=60)
                                                                                                                        GCGAGTTTTCGCATCCGGTTATCGTCTGCTCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCTAACGGCGCGACTTTTAT                               <  SRR19973065.1477030/150‑1 (MQ=60)
                                                                                                                        GCGAGTTTTCGCATCCGGTTATCGTCTGCTCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCTAACGGCGCGACTTTTAT                               <  SRR19973065.1477013/150‑1 (MQ=60)
                                                                                                                         CGAGTTTTCGCATCCGGTTATCGTCTGCTCCACCACAACATTCCCATCATTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGGGGTGTAACGAGACAATGCTAACGGCGCGACTTTTATC                              >  SRR19973065.2901625/1‑150 (MQ=60)
                                                                                                                         CGAGTTTTCGCATCCGGTTATCGTCTGCTCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCTAACGGCGCGACTTTTATC                              <  SRR19973065.3145237/150‑1 (MQ=60)
                                                                                                                         CGAGTTTTCGCATCCGGTTATCGTCTGCTCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCTAACGGCGCGACTTTTATC                              <  SRR19973065.3145192/150‑1 (MQ=60)
                                                                                                                         CGAGTTTTCGCATCCGGTTATCGTCTGCTCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCTAACGGCGCGACTTTTATC                              >  SRR19973065.1067954/1‑150 (MQ=60)
                                                                                                                          GAGTTTTCGCATCCGGTTATCGTCTGCTCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCTAACGGCGCGACTTTTATCA                             <  SRR19973065.3374551/150‑1 (MQ=60)
                                                                                                                          GAGTTTTCGCATCCGGTTATCGTCTGCTCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCTAACGGCGCGACTTTTATCA                             <  SRR19973065.3374633/150‑1 (MQ=60)
                                                                                                                            GTTTTCGCATCCGGTTATCGTCTGCTCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCTAACGGCGCGACTTTTATCACC                           <  SRR19973065.194403/150‑1 (MQ=60)
                                                                                                                              TTTCGCATCCGGTTATCGTCTGCTCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCTAACGGCGCGACTTTTATCACCTG                         >  SRR19973065.2202771/1‑150 (MQ=60)
                                                                                                                                   CATCCGGTTATCGTCTGCTCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCTAACGGCGCGACTTTTATCACCTGCCAGG                    >  SRR19973065.1775168/1‑150 (MQ=60)
                                                                                                                                   CATCCGGTTATCGTCTGCTCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCTAACGGCGCGACTTTTATCACCTGCCAGG                    >  SRR19973065.3083369/1‑150 (MQ=60)
                                                                                                                                   CATCCGGTTATCGTCTGCTCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCTAACGGCGCGACTTTTATCACCTGCCAGG                    <  SRR19973065.680375/150‑1 (MQ=60)
                                                                                                                                   CATCCGGTTATCGTCTGCTCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCTAACGGCGCGACTTTTATCACCTGCCAGG                    <  SRR19973065.3171857/150‑1 (MQ=60)
                                                                                                                                    ATCCGGTTATCGTCTGCTCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCTAACGGCGCGACTTTTATCACCTGCCAGGG                   >  SRR19973065.1117738/1‑150 (MQ=60)
                                                                                                                                    ATCCGGTTATCGTCTGCTCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCTAACGGCGCGACTTTTATCACCTGCCAGGG                   >  SRR19973065.804582/1‑150 (MQ=60)
                                                                                                                                    ATCCGGTTATCGTCTGCTCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCTAACGGCGCGACTTTTATCACCTGCCAGGG                   >  SRR19973065.1701657/1‑150 (MQ=60)
                                                                                                                                    ATCCGGTTATCGTCTGCTCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCTAACGGCGCGACTTTTATCACCTGCCAGGG                   >  SRR19973065.1700828/1‑150 (MQ=60)
                                                                                                                                      CCGGTTATCGTCTGCTCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCTAACGGCGCGACTTTTATCACCTGCCAGGGAG                 <  SRR19973065.286126/150‑1 (MQ=60)
                                                                                                                                      CCGGTTATCGTCTGCTCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCTAACGGCGCGACTTTTATCACCTGCCAGGGAG                 <  SRR19973065.2100905/150‑1 (MQ=60)
                                                                                                                                      CCGGTTATCGTCTGCTCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCTAACGGCGCGACTTTTATCACCTGCCAGGGAG                 <  SRR19973065.286599/150‑1 (MQ=60)
                                                                                                                                      CCGGTTATCGTCTGCTCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCTAACGGCGCGACTTTTATCACCTGCCAGGGAG                 <  SRR19973065.286124/150‑1 (MQ=60)
                                                                                                                                      CCGGTTATCGTCTGCTCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCTAACGGCGCGACTTTTATCACCTGCCAGGGAG                 <  SRR19973065.2823639/150‑1 (MQ=60)
                                                                                                                                      CCGGTTATCGTCTGCTCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCTAACGGCGCGACTTTTATCACCTGCCAGGGAG                 <  SRR19973065.286125/150‑1 (MQ=60)
                                                                                                                                      CCGGTTATCGTCTGCTCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCTAACGGCGCGACTTTTATCACCTGCCAGGGAG                 <  SRR19973065.2100026/150‑1 (MQ=60)
                                                                                                                                      CCGGTTATCGTCTGCTCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATAAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCTAACGGCGCGACTTTTATCACCTGCCAGGGAG                 <  SRR19973065.611946/150‑1 (MQ=60)
                                                                                                                                      CCGGTTATCGTCTGCTCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACAACAATACACAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCTAACGGCGCGACTTTTATCACCTGCCAGGGAG                 <  SRR19973065.611757/150‑1 (MQ=60)
                                                                                                                                        GGTTATCGTCTGCTCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCTAACGGCGCGACTTTTATCACCTGCCAGGGAGCG               >  SRR19973065.3409260/1‑150 (MQ=60)
                                                                                                                                        GGTTATCGTCTGCTCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCTAACGGCGCGACTTTTATCACCTGCCAGGGAGCG               >  SRR19973065.2552730/1‑150 (MQ=60)
                                                                                                                                        GGTTATCGTCTGCTCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCTAACGGCGCGACTTTTATCACCTGCCAGGGAGCG               >  SRR19973065.267435/1‑150 (MQ=60)
                                                                                                                                            ATCGTCTGCTCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCTAACGGCGCGACTTTTATCACCTGCCAGGGAGCGAAAA           >  SRR19973065.848706/1‑150 (MQ=60)
                                                                                                                                              CGTCTGCTCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCTAACGGCGCGACTTTTATCACCTGCCAGGGAGCGAAAAAG         <  SRR19973065.825143/150‑1 (MQ=60)
                                                                                                                                                  TGCTCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCTAACGGCGCGACTTTTATCACCTGCCAGGGAGCGAAAAAGCGTT     >  SRR19973065.590791/1‑150 (MQ=60)
                                                                                                                                                   GCTCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCTAACGGCGCGACTTTTATCACCTGCCAGGGAGCGAAAAAGCGTTC    >  SRR19973065.586349/1‑150 (MQ=60)
                                                                                                                                                   GCTCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCTAACGGCGCGACTTTTATCACCTGCCAGGGAGCGAAAAAGCGTTC    >  SRR19973065.586348/1‑150 (MQ=60)
                                                                                                                                                     TCCACCACAACAGTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCTAACGGCGCGACTTTTATCACCTGCCAGGGAGCGAAAAAGCGTTCAT  <  SRR19973065.3474600/150‑1 (MQ=60)
                                                                                                                                                     |                                                                                                                                                     
AGCGAAAGACTGGGCGGCGGCGGATGCGGCGCGTGATCGTCTTAACGAGATGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCAAACGGCGCGACTTTTATCACCTGCCAGGGAGCGAAAAAGCGTTCAT  >  CP009273/551350‑551648

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑