Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F434 I0 R1 1130 215.9 7031662 97.1% 6827743 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 601,847 A→T 100% I331I (ATT→ATA)  ybdK ← weak gamma‑glutamyl:cysteine ligase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273601,8470AT97.0% 749.6 / 9.1 232I331I (ATT→ATA) ybdKweak gamma‑glutamyl:cysteine ligase
Reads supporting (aligned to +/- strand):  ref base A (3/4);  new base T (104/121);  total (107/125)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.87e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 232 total aligned reads displayed.

TAAGGCAATTCCCGGCTTAGTCACCGGCCCAGATCTCACAATGCTTTTTCACCAGCCCAATCAGCGAGCCGCCATCGGCGACGAAATCGCGCATTAGCTGCGCTTCATTCAGACCGCTGACGACCTGGCGATGCAGGGCCTCAATCGCGCTCGATGCACCAATTTTATGTGCGGAAGGGGCGATTTTTTCCAGCAATCGCAAGGTATCTTCCGTTAGCGGTCGACGATCTCCAGTGTGCGGATCGGTGATGACGCCTTCAAGCCCATAGCGACAGGCCTGGAAACG  >  CP009273/601705‑601990
                                                                                                                                              |                                                                                                                                               
tAAGGCAATTCCCGGCTTAGTCACCGGCCCAGATCTCACAATGCTTTTTCACCAGCCCAATCAGCGAGCCGCCATCGGCGACGAAATCGCGCATTAGCTGCGCTTCATTCAGACCGCTGACGACCTGGCGATGCAGGGCCTCTATcgc                                                                                                                                            >  1:1924131/1‑148 (MQ=255)
 aaGGCAATTCCCGGCTTAGTCACCGGCCCAGATCTCACAATGCTTTTTCACCAGCCCAATCAGCGAGCCGCCATCGGCGACGAAATCGCGCATTAGCTGCGCTTCATTCAGACCGCTGACGACCTGGCGATGCAGGGCCTCTATcgcg                                                                                                                                           <  2:2654284/148‑1 (MQ=255)
  aGGCAATTCCCGGCTTAGTCACCGGCCCAGATCTCACAATGCTTTTTCACCAGCCCAATCAGCGAGCCGCCATCGGCGACGAAATCGCGCATTAGCTGCGCTTCATTCAGACCGCTGACGACCTGGCGATGCAGGGCCTCTATCgcgc                                                                                                                                          <  1:309962/148‑1 (MQ=255)
  aGGCAATTCCCGGCTTAGTCACCGGCCCAGATCTCACAATGCTTTTTCACCAGCCCAATCAGCGAGCCGCCATCGGCGACGAAATCGCGCATTAGCTGCGCTTCATTCAGACCGCTGACGACCTGGCGATGCAGGGCCTCTATCgcgc                                                                                                                                          <  2:196449/148‑1 (MQ=255)
  aGGCAATTCCCGGCTTAGTCACCGGCCCAGATCTCACAATGCTTTTTCACCAGCCCAATCAGCGAGCCGCCATCGGCGACGAAATCGCGCATTAGCTGCGCTTCATTCAGACCGCTGACGACCTGGCGATGCAGGGCCTCTATCgcgc                                                                                                                                          >  2:2864371/1‑148 (MQ=255)
   ggCAATTCCCGGCTTAGTCACCGGCCCAGATCTCACAATGCTTTTTCACCAGCCCAATCAGCGAGCCGCCATCGGCGACGAAATCGCGCATTAGCTGCGCTTCATTCAGACCGCTGACGACCTGGCGATGCAGGGCCTCTATCGCGCt                                                                                                                                         <  1:2672038/148‑1 (MQ=255)
   ggCAATTCCCGGCTTAGTCACCGGCCCAGATCTCACAATGCTTTTTCACCAGCCCAATCAGCGAGCCGCCATCGGCGACGAAATCGCGCATTAGCTGCGCTTCATTCAGACCGCTGACGACCTGGCGATGCAGGGCCTCTATCGCGCt                                                                                                                                         <  1:199015/148‑1 (MQ=255)
     cAATTCCCGGCTTAGTCACCGGCCCAGATCTCACAATGCTTTTTCACCAGCCCAATCAGCGAGCCGCCATCGGCGACGAAATCGCGCATTAGCTGCGCTTCATTCAGACCGCTGACGACCTGGCGATGCAGGGCCTCTATCGCGCTCg                                                                                                                                       >  1:3436820/1‑148 (MQ=255)
     cAATTCCCGGCTTAGTCACCGGCCCAGATCTCACAATGCTTTTTCACCAGCCCAATCAGCGAGCCGCCATCGGCGACGAAATCGCGCATTAGCTGCGCTTCATTCAGACCGCTGACGACCTGGCGATGCAGGGCCTCTATCGCGCTCg                                                                                                                                       <  1:3090706/148‑1 (MQ=255)
        ttCCCGGCTTAGTCACCGGCCCAGATCTCACAATGCTTTTTCACCAGCCCAATCAGCGAGCCGCCATCGGCGACGAAATCGCGCATTAGCTGCGCTTCATTCAGACCGCTGACGACCTGGCGATGCAGGGCCTCTATCGCGCTCGATg                                                                                                                                    <  2:3442634/148‑1 (MQ=255)
         tCCCGGCTTAGTCACCGGCCCAGATCTCACAATGCTTTTTCACCAGCCCAATCAGCGAGCCGCCATCGGCGACGAAATCGCGCATTAGCTGCGCTTCATTCAGACCGCTGACGACCTGGCGATGCAGGGCCTCTATCGCGCTCGATGc                                                                                                                                   >  2:124753/1‑148 (MQ=255)
          cccGGCTTAGTCACCGGCCCAGATCTCACAATGCTTTTTCACCAGCCCAATCAGCGAGCCGCCATCGGCGACGAAATCGCGCATTAGCTGCGCTTCATTCAGACCGCTGACGACCTGGCGATGCAGGGCCTCTATCGCGCTCGATGCa                                                                                                                                  >  1:1354522/1‑148 (MQ=255)
           ccGGCTTAGTCACCGGCCCAGATCTCACAATGCTTTTTCACCAGCCCAATCAGCGAGCCGCCATCGGCGACGAAATCGCGCATTAGCTGCGCTTCATTCAGACCGCTGACGACCTGGCGATGCAGGGCCTCTATCGCGCTCGATGCAc                                                                                                                                 <  1:751137/148‑1 (MQ=255)
           ccGGCTTAGTCACCGGCCCAGATCTCACAATGCTTTTTCACCAGCCCAATCAGCGAGCCGCCATCGGCGACGAAATCGCGCATTAGCTGCGCTTCATTCAGACCGCTGACGACCTGGCGATGCAGGGCCTCTATCGCGCTCGATGCAc                                                                                                                                 >  2:636083/1‑148 (MQ=255)
             ggCTTAGTCACCGGCCCAGATCTCACAATGCTTTTTCACCAGCCCAATCAGCGAGCCGCCATCGGCGACGAAATCGCGCATTAGCTGCGCTTCATTCAGACCGCTGACGACCTGGCGATGCAGGGCCTCTATCGCGCTCGATGCACCa                                                                                                                               >  1:775638/1‑148 (MQ=255)
             ggCTTAGTCACCGGCCCAGATCTCACAATGCTTTTTCACCAGCCCAATCAGCGAGCCGCCATCGGCGACGAAATCGCGCATTAGCTGCGCTTCATTCAGACCGCTGACGACCTGGCGATGCAGGGCCTCTATCGCGCTCGATGCACCa                                                                                                                               <  1:3159865/148‑1 (MQ=255)
                  aGTCACCGGCCCAGATCTCACAATGCTTTTTCACCAGCCCAATCAGCGAGCCGCCATCGGCGACGAAATCGCGCATTAGCTGCGCTTCATTCAGACCGCTGACGACCTGGCGATGCAGGGCCTCTATCGCGCTCGATGCACCAAtttt                                                                                                                          <  1:3017648/148‑1 (MQ=255)
                   gTCACCGGCCCAGATCTCACAATGCTTTTTCACCAGCCCAATCAGCGAGCCGCCATCGGCGACGAAATCGCGCATTAGCTGCGCTTCATTCAGACCGCTGACGACCTGGCGATGCAGGGCCTCTATCGCGCTCGATGCACCAATTTTa                                                                                                                         >  2:1086287/1‑148 (MQ=255)
                   gTCACCGGCCCAGATCTCACAATGCTTTTTCACCAGCCCAATCAGCGAGCCGCCATCGGCGACGAAATCGCGCATTAGCTGCGCTTCATTCAGACCGCTGACGACCTGGCGATGCAGGGCCTCTATCGCGCTCGATGCACCAATTTTa                                                                                                                         <  2:1162134/148‑1 (MQ=255)
                   gTCACCGGCCCAGATCTCACAATGCTTTTTCACCAGCCCAATCAGCGAGCCGCCATCGGCGACGAAATCGCGCATTAGCTGCGCTTCATTCAGACCGCTGACGACCTGGCGATGCAGGGCCTCTATCGCGCTCGATGCACCAATTTTa                                                                                                                         <  2:2837760/148‑1 (MQ=255)
                     cACCGGCCCAGATCTCACAATGCTTTTTCACCAGCCCAATCAGCGAGCCGCCATCGGCGACGAAATCGCGCATTAGCTGCGCTTCATTCAGACCGCTGACGACCTGGCGATGCAGGGCCTCTATCGCGCTCGATGCACCAATTTTAtg                                                                                                                       <  1:97506/148‑1 (MQ=255)
                     cACCGGCCCAGATCTCACAATGCTTTTTCACCAGCCCAATCAGCGAGCCGCCATCGGCGACGAAATCGCGCATTAGCTGCGCTTCATTCAGACCGCTGACGACCTGGCGATGCAGGGCCTCTATCGCGCTCGATGCACCAATTTTAtg                                                                                                                       >  1:3374219/1‑148 (MQ=255)
                       ccGGCCCAGATCTCACAATGCTTTTTCACCAGCCCAATCAGCGAGCCGCCATCGGCGACGAAATCGCGCATTAGCTGCGCTTCATTCAGACCGCTGACGACCTGGCGATGCAGGGCCTCTATCGCGCTCGATGCACCAATTTTAtgtg                                                                                                                     <  2:223148/148‑1 (MQ=255)
                        cGGCCCAGATCTCACAATGCTTTTTCACCAGCCCAATCAGCGAGCCGCCATCGGCGACGAAATCGCGCATTAGCTGCGCTTCATTCAGACCGCTGACGACCTGGCGATGCAGGGCCTCTATCGCGCTCGATGCACCAATTTTATGTGc                                                                                                                    >  2:1036157/1‑148 (MQ=255)
                               gATCTCACAATGCTTTTTCACCAGCCCAATCAGCGAGCCGCCATCGGCGACGAAATCGCGCATTAGCTGCGCTTCATTCAGACCGCTGACGACCTGGCGATGCAGGGCCTCTATCGCGCTCGATGCACCAATTTTATGTGCGGAAggg                                                                                                             <  2:3514463/148‑1 (MQ=255)
                               gATCTCACAATGCTTTTTCACCAGCCCAATCAGCGAGCCGCCATCGGCGACGAAATCGCGCATTAGCTGCGCTTCATTCAGACCGCTGACGACCTGGCGATGCAGGGCCTCTATCGCGCTCGATGCACCAATTTTATGTGCGGAAggg                                                                                                             >  2:2056738/1‑148 (MQ=255)
                               gATCTCACAATGCTTTTTCACCAGCCCAATCAGCGAGCCGCCATCGGCGACGAAATCGCGCATTAGCTGCGCTTCATTCAGACCGCTGACGACCTGGCGATGCAGGGCCTCTATCGCGCTCGATGCACCAATTTTATGTGCGGAAggg                                                                                                             <  2:1111564/148‑1 (MQ=255)
                               gATCTCACAATGCTTTTTCACCAGCCCAATCAGCGAGCCGCCATCGGCGACGAAATCGCGCATTAGCTGCGCTTCATTCAGACCGCTGACGACCTGGCGATGCAGGGCCTCTATCGCGCTCGATGCACCAATTTTATGTGCGGAAggg                                                                                                             <  2:1895945/148‑1 (MQ=255)
                               gATCTCACAATGCTTTTTCACCAGCCCAATCAGCGAGCCGCCATCGGCGACGAAATCGCGCATTAGCTGCGCTTCATTCAGACCGCTGACGACCTGGCGATGCAGGGCCTCTATCGCGCTCGATGCACCAATTTTATGTGCGGAAggg                                                                                                             <  2:1895401/148‑1 (MQ=255)
                                aTCTCACAATGCTTTTTCACCAGCCCAATCAGCGAGCCGCCATCGGCGACGAAATCGCGCATTAGCTGCGCTTCATTCAGACCGCTGACGACCTGGCGATGCAGGGCCTCTATCGCGCTCGATGCACCAATTTTATGTGCGGAAgggg                                                                                                            >  2:3008187/1‑148 (MQ=255)
                                 tctcACAATGCTTTTTCACCAGCCCAATCAGCGAGCCGCCATCGGCGACGAAATCGCGCATTAGCTGCGCTTCATTCAGACCGCTGACGACCTGGCGATGCAGGGCCTCTATCGCGCTCGATGCACCAATTTTATGTGCGGAAGGGGc                                                                                                           <  2:548984/148‑1 (MQ=255)
                                      caATGCTTTTTCACCAGCCCAATCAGCGAGCCGCCATCGGCGACGAAATCGCGCATTAGCTGCGCTTCATTCAGACCGCTGACGACCTGGCGATGCAGGGCCTCTATCGCGCTCGATGCACCAATTTTATGTGCGGAAGGGGCGAttt                                                                                                      <  1:639405/148‑1 (MQ=255)
                                         tGCTTTTTCACCAGCCCAATCAGCGAGCCGCCATCGGCGACGAAATCGCGCATTAGCTGCGCTTCATTCAGACCGCTGACGACCTGGCGATGCAGGGCCTCTATCGCGCTCGATGCACCAATTTTATGTGCGGAAGGGGCGAtttttt                                                                                                   >  1:1218135/1‑148 (MQ=255)
                                           cTTTTTCACCAGCCCAATCAGCGAGCCGCCATCGGCGACGAAATCGCGCATTAGCTGCGCTTCATTCAGACCGCTGACGACCTGGCGATGCAGGGCCTCTATCGCGCTCGATGCACCAATTTTATGTGCGGAAGGGGCGATTTTTTcc                                                                                                 <  1:3034131/148‑1 (MQ=255)
                                                tCACCAGCCCAATCAGCGAGCCGCCATCGGCGACGAAATCGCGCATTAGCTGCGCTTCATTCAGACCGCTGACGACCTGGCGATGCAGGGCCTCTATCGCGCTCGATGCACCAATTTTATGTGCGGAAGGGGCGATTTTTTCCAGCaa                                                                                            <  2:918420/148‑1 (MQ=255)
                                                 cACCAGCCCAATCAGCGAGCCGCCATCGGCGACGAAATCGCGCATTAGCTGCGCTTCATTCAGACCGCTGACGACCTGGCGATGCAGGGCCTCTATCGCGCTCGATGCACCAATTTTATGTGCGGAAGGGGCGATTTTTTCCAGCAAt                                                                                           <  1:1617456/148‑1 (MQ=255)
                                                    cAGCCCAATCAGCGAGCCGCCATCGGCGACGAAATCGCGCATTAGCTGCGCTTCATTCAGACCGCTGACGACCTGGCGATGCAGGGCCTCTATCGCGCTCGATGCACCAATTTTATGTGCGGAAGGGGCGATTTTTTCCAGCAATCGc                                                                                        <  1:2626327/148‑1 (MQ=255)
                                                      gCCCAATCAGCGAGCCGCCATCGGCGACGAAATCGCGCATTAGCTGCGCTTCATTCAGACCGCTGACGACCTGGCGATGCAGGGCCTCTATCGCGCTCGATGCACCAATTTTATGTGCGGAAGGGGCGATTTTTTCCAGCAATCGCaa                                                                                      >  2:1283927/1‑148 (MQ=255)
                                                          aaTCAGCGAGCCGCCATCGGCGACGAAATAGCGCATTAGCTGCGCTTCATTCAGACCGCTGACGACCTGGCGATGCAGGGCCTCTATCGCGCTCGATGCACCAATTTTATGTGCGGAAGGGGCGATTTTTTCCAGCAATCGCAAGGTa                                                                                  >  1:1191289/1‑148 (MQ=255)
                                                           aTCAGCGAGCCGCCATCGGCGACGAAATCGCGCATTAGCTGCGCTTCATTCAGACCGCTGACGACCTGGCGATGCAGGGCCTCTATCGCGCTCGATGCACCAATTTTATGTGCGGAAGGGGCGATTTTTTCCAGCAATCGCAAGGTAt                                                                                 >  1:2613182/1‑148 (MQ=255)
                                                            tCAGCGAGCCGCCATCGGCGACGAAATCGCGCATTAGCTGCGCTTCATTCAGACCGCTGACGACCTGGCGATGCAGGGCCTCTATCGCGCTCGATGCACCAATTTTATGTGCGGAAGGGGCGATTTTTTCCAGCAATCGCAAGGTATc                                                                                >  1:356101/1‑148 (MQ=255)
                                                            tCAGCGAGCCGCCATCGGCGACGAAATCGCGCATTAGCTGCGCTTCATTCAGACCGCTGACGACCTGGCGATGCAGGGCCTCTATCGCGCTCGATGCACCAATTTTATGTGCGGAAGGGGCGATTTTTTCCAGCAATCGCAAGGTATc                                                                                <  1:389024/148‑1 (MQ=255)
                                                              aGCGAGCCGCCATCGGCGACGAAATCGCGCATTAGCTGCGCTTCATTCAGACCGCTGACGACCTGGCGATGCAGGGCCTCTATCGCGCTCGATGCACCAATTTTATGTGCGGAAGGGGCGATTTTTTCCAGCAATCGCAAGGTATCtt                                                                              <  1:2472659/148‑1 (MQ=255)
                                                               gCGAGCCGCCATCGGCGACGAAATCGCGCATTAGCTGCGCTTCATTCAGACCGCTGACGACCTGGCGATGCAGGGCCTCTATCGCGCTCGATGCACCAATTTTATGTGCGGAAGGGGCGATTTTTTCCAGCAATCGCAAGGTATCTTc                                                                             <  2:995059/148‑1 (MQ=255)
                                                                cGAGCCGCCATCGGCGACGAAATCGCGCATTAGCTGCGCTTCATTCAGACCGCTGACGACCTGGCGATGCAGGGCCTCTATCGCGCTCGATGCACCAATTTTATGTGCGGAAGGGGCGATTTTTTCCAGCAATCGCAAGGTATCTTcc                                                                            >  1:3274563/1‑148 (MQ=255)
                                                                cGAGCCGCCATCGGCGACGAAATCGCGCATTAGCTGCGCTTCATTCAGACCGCTGACGACCTGGCGATGCAGGGCCTCTATCGCGCTCGATGCACCAATTTTATGTGCGGAAGGGGCGATTTTTTCCAGCAATCGCAAGGTATCTTcc                                                                            <  1:1148982/148‑1 (MQ=255)
                                                                 gAGCCGCCATCGGCGACGAAATCGCGCATTAGCTGCGCTTCATTCAGACCGCTGACGACCTGGCGATGCAGGGCCTCTATCGCGCTCGATGCACCAATTTTATGTGCGGAAGGGGCGATTTTTTCCAGCAATCGCAAGGTATCTTCCg                                                                           <  2:2892766/148‑1 (MQ=255)
                                                                   gccgccATCGGCGACGAAATCGCGAATTAGCTGCGCTTCATTCATACCGCTGACGAACTGGCTATGCAGGGCCTCTATCTAGCTCGATGAACCAATTTTATGTGCGGAAGGTGCGATTTTTTCCAGCAAACGCAAGGTATCTTCCgat                                                                         >  2:3192517/1‑146 (MQ=255)
                                                                    ccgccATCGGCGACGAAATCGCGCATGAGCTGCGCTTCATTCAGACCGCTGACGACCTGGCGATGCAGGGCCTCTATCGCGCTCGATGCACCAATTTTATGTGCGGAAGGGGCGATTTTTTCCAGCAATCGCAAGGTATCTTCCGTTa                                                                        <  2:1805809/148‑1 (MQ=255)
                                                                       ccATCGGCGACGAAATCGCGCATTAGCTGCGCTTCATTCAGACCGCTGACGACCTGGCGATGCAGGGCCTCTATCGCGCTCGATGCACCAATTTTATGTGCGGAAGGGGCGATTTTTTCCAGCAATCGCAAGGTATCTTCCGTTAGCg                                                                     >  2:439840/1‑148 (MQ=255)
                                                                       ccATCGGCGACGAAATCGCGCATTAGCTGCGCTTCATTCAGACCGCTGACGACCTGGCGATGCAGGGCCTCTATCGCGCTCGATGCACCAATTTTATGTGCGGAAGGGGCGATTTTTTCCAGCAATCGCAAGGTATCTTCCGTTAGCg                                                                     >  2:1876590/1‑148 (MQ=255)
                                                                        cATCGGCGACGAAATCGCGCATTAGCTGCGCTTCATTCAGACCGCTGACGACCTGGCGATGCAGGGCCTCTATCGCGCTCGATGCACCAATTTTATGTGCGGAAGGGGCGATTTTTTCCAGCAATCGCAAGGTATCTTCCGTTAGCgg                                                                    >  2:2838596/1‑148 (MQ=255)
                                                                            ggCGACGAAATCGCGCATTAGCTGCGCTTCATTCAGACCGCTGACGACCTGGCGATGCAGGGCCTCTATCGCGCTCGATGCACCAATTTTATGTGCGGAAGGGGCGATTTTTTCCAGCAATCGCAAGGTATCTTCCGTTAGCGGTcga                                                                >  1:724667/1‑148 (MQ=255)
                                                                              cgacgaAATCGCGCATTAGCTGCGCTTCATTCAGACCGCTGACGACCTGGCGATGCAGGGCCTCTATCGCGCTCGATGCACCAATTTTATGTGCGGAAGGGGCGATTTTTTCCAGCAATCGCAAGGTATCTTCCGTTAGCGGTcgacg                                                              <  2:2694747/148‑1 (MQ=255)
                                                                                acgaAATCGCGCATTAGCTGCGCTTCATTCAGACCGCTGACGACCTGGCGATGCAGGGCCTCTATCGCGCTCGATGCACCAATTTTATGTGCGGAAGGGGCGATTTTTTCCAGCAATCGCAAGGTATCTTCCGTTAGCGGTCGACGAt                                                            <  1:496555/148‑1 (MQ=255)
                                                                                acgaAATCGCGCATTAGCTGCGCTTCATTCAGACCGCTGACGACCTGGCGATGCAGGGCCTCTATCGCGCTCGATGCACCAATTTTATGTGCGGAAGGGGCGATTTTTTCCAGCAATCGCAAGGTATCTTCCGTTAGCGGTCGACGAt                                                            >  2:214065/1‑148 (MQ=255)
                                                                                 cgaAATCGCGCATTAGCTGCGCTTCATTCAGACCGCTGACGACCTGGCGATGCAGGGCCTCTATCGCGCTCGATGCACCAATTTTATGTGCGGAAGGGGCGATTTTTTCCAGCAATCGCAAGGTATCTTCCGTTAGCGGTCGACGAtc                                                           <  2:2668602/148‑1 (MQ=255)
                                                                                   aaaTCGCGCATTAGCTGCGCTTCATTCAGACCGCTGACGACCTGGCGATGCAGGGCCTCTATCGCGCTCGATGCACCAATTTTATGTGCGGAAGGGGCGATTTTTTCCAGCAATCGCAAGGTATCTTCCGTTAGCGGTCGACGAtctc                                                         <  1:1619852/148‑1 (MQ=255)
                                                                                   aaaTCGCGCATTAGCTGCGCTTCATTCAGACCGCTGACGACCTGGCGATGCAGGGCCTCTATCGCGCTCGATGCACCAATTTTATGTGCGGAAGGGGCGATTTTTTCCAGCAATCGCAAGGTATCTTCCGTTAGCGGTCGACGAtctc                                                         >  1:770064/1‑148 (MQ=255)
                                                                                    aaTCGCGCATTAGCTGCGCTTCATTCAGACCGCTGACGACCTGGCGATGCAGGGCCTCTATCGCGCTCGATGCACCAATTTTATGTGCGGAAGGGGCGATTTTTTCCAGCAATCGCAAGGTATCTTCCGTTAGCGGTCGACGATCTcc                                                        <  1:3008187/148‑1 (MQ=255)
                                                                                     aTCGCGCATTAGCTGCGCTTCATTCAGACCGCTGACGACCTGGCGATGCAGGGCCTCTATCGCGCTCGATGCACCAATTTTATGTGCGGAAGGGGCGATTTTTTCCAGCAATCGCAAGGTATCTTCCGTTAGCGGTCGACGATCTCCa                                                       <  1:1002541/148‑1 (MQ=255)
                                                                                      tCGCGCATTAGCTGCGCTTCATTCAGACCGCTGACGACCTGGCGATGCAGGGCCTCTATCGCGCTCGATGCACCAATTTTATGTGCGGAAGGGGCGATTTTTTCCAGCAATCGCAAGGTATCTTCCGTTAGCGGTCGACGATCTCCAg                                                      >  2:3024362/1‑148 (MQ=255)
                                                                                       cgcgCATTAGCTGCGCTTCATTCAGACCGCTGACGACCTGGCGATGCAGGGCCTCTATCGCGCTCGATGCACCAATTTTATGTGCGGAAGGGGCGATTTTTTCCAGCAATCGCAAGGTATCTTCCGTTAGCGGTCGACGATCTCCAgt                                                     >  2:821845/1‑148 (MQ=255)
                                                                                        gcgcATTAGCTGCGCTTCATTCAGACCGCTGACGACCTGGCGATGCAGGGCCTCTATCGCGCTCGATGCACCAATTTTATGTGCGGAAGGGGCGATTTTTTCCAGCAATCGCAAGGTATCTTCCGTTAGCGGTCGACGATCTCCAgtg                                                    <  2:1763806/148‑1 (MQ=255)
                                                                                          gcATTAGCTGCGCTTCATTCAGACCGCTGACGACCTGGCGATGCAGGGCCTCTATCGCGCTCGATGCACCAATTTTATGTGCGGAAGGGGCGATTTTTTCCAGCAATCGCAAGGTATCTTCCGTTAGCGGTCGACGATCTCCAGtgtg                                                  <  1:2864371/148‑1 (MQ=255)
                                                                                             ttAGCTGCGCTTCATTCAGACCGCTGACGACCTGGCGATGCAGGGCCTCTATCGCGCTCGATGCACCAATTTTATGTGCGGAAGGGGCGATTTTTTCCAGCAATCGCACGGTATCTTCCGTTAGCGGTCGACGATCTCCAGTGTGCgg                                               >  2:2388682/1‑148 (MQ=255)
                                                                                             ttAGCTGCGCTTCATTCAGACCGCTGACGACCTGGCGATGCAGGGCCTCTATCGCGCTCGATGCACCAATTTTATGTGCGGAAGGGGCGATTTTTTCCAGCAATCGCAAGGTATCTTCCGTTAGCGGTCGACGATCTCCAGTGTGCgg                                               >  1:631909/1‑148 (MQ=255)
                                                                                              tAGCTGCGCTTCATTCAGACCGCTGACGACCTGGCGATGCAGGGCCTCTATCGCGCTCGATGCACCAATTTTATGTGCGGAAGGGGCGATTTTTTCCAGCAATCTCAAGGTATCTTCCGTTAGCGGTCGACGATCTCCAGTGTGCGGa                                              <  2:2319245/148‑1 (MQ=255)
                                                                                                 cTGCGCTTCATTCAGACCGCTGACGACCTGGCGATGCAGGGCCTCTATCGCGCTCGATGCACCAATTTTATGTGCGGAAGGGGCGATTTTTTCCAGCAATCGCAAGGTATCTTCCGTTAGCGGTCGACGATCTCCAGTGTGCGGATCg                                           >  2:958602/1‑148 (MQ=255)
                                                                                                 cTGCGCTTCATTCAGACCGCTGACGACCTGGCGATGCAGGGCCTCTATCGCGCTCGATGCACCAATTTTATGTGCGGAAGGGGCGATTTTTTCCAGCAATCGCAAGGTATCTTCCGTTAGCGGTCGACGATCTCCAGTGTGCGGATCg                                           >  1:3440594/1‑148 (MQ=255)
                                                                                                 cTGCGCTTCATTCAGACCGCTGACGACCTGGCGATGCAGGGCCTCTATCGCGCTCGATGCACCAATTTTATGTGCGGAAGGGGCGATTTTTTCCAGCAATCGCAAGGTATCTTCAGTTAGCGGTCGACGATCTCCAGTGTGCGGATCg                                           >  2:1602620/1‑148 (MQ=255)
                                                                                                   gcgcTTCATTCAGACCGCTGACGACCTGGCGATGCAGGGCCTCTATCGCGCTCGATGCACCAATTTTATGTGCGGAAGGGGCGATTTTTTCCAGCAATCGCAAGGTATCTTCCGTTAGCGGTCGACGATCTCCAGTGTGCGGATCGGt                                         <  2:2420595/148‑1 (MQ=255)
                                                                                                   gcgcTTCATTCAGACCGCTGACGACCTGGCGATGCAGGGCCTCTATCGCGCTCGATGCACCAATTTTATGTGCGGAAGGGGCGATTTTTTCCAGCAATCGCAAGGTATCTTCCGTTAGCGGTCGACGATCTCCAGTGTGCGGATCGGt                                         <  1:1034004/148‑1 (MQ=255)
                                                                                                      cTTCATTCAGACCGCTGACGACCTGGCGATGCAGGGCCTCAATCGCGCTCGATGCACCAATTTTATGTGCGGAAGGGGCGATTTTTTCCAGCAATCGCAAGGTATCTTCCGTTAGCGGTCGACGATCTCCAGTGTGCGGATCGGtgat                                      >  2:525801/1‑148 (MQ=255)
                                                                                                      cTTCATTCAGACCGCTGACGACCTGGCGATGCAGGGCCTCAATCGCGCTCGATGCACCAATTTTATGTGCGGAAGGGGCGATTTTTTCCAGCAATCGCAAGGTATCTTCCGTTAGCGGTCGACGATCTCCAGTGTGCGGATCGGtgat                                      >  2:2289881/1‑148 (MQ=255)
                                                                                                       ttcattcaGACCGCTGACGACCTGGCGATGCAGGGCCTCTATCGCGCTCGATGCACCAATTTTATGTGCGGAAGTGGAGATTTTTTCCAGCAATCGCAATGTATCTTGCGTTAGCGGTCGACTATCTCCAGTGTGCGGATCGGtgatg                                     <  1:188151/148‑1 (MQ=255)
                                                                                                            tcaGACCGCTGACGACCTGGCGATGCAGGGCCTCTATCGCGCTCGATGCACCAATTTTATGTGCGGAAGGGGCGATTTTTTCCAGCAATCGCAAGGTATCTTCCGTTAGCGGTCGACGATCTCCAGTGTGCGGATCGGTGATGACGcc                                <  1:1082117/148‑1 (MQ=255)
                                                                                                            tcaGACCGCTGACGACCTGGCGATGCAGGGCCTCTATCGCGCTCGATGCACCAATTTTATGTGCGGAAGGGGCGATTTTTTCCAGCAATCGCAAGGTATCTTCCGTTAGCGGTCGACGATCTCCAGTGTGCGGATCGGTGATGACGcc                                >  2:1233643/1‑148 (MQ=255)
                                                                                                            tcaGACCGCTGACGACCTGGCGATGCAGGGCCTCTATCGCGCTCGATGCACCAATTTTATGTGCGGAAGGGGCGATTTTTTCCAGCAATCGCAAGGTATCTTCCGTTAGCGGTCGACGATCTCCAGTGTGCGGATCGGTGATGACGcc                                <  2:394108/148‑1 (MQ=255)
                                                                                                            tcaGACCGCTGACGACCTGGCGATGCAGGGCCTCTATCGCGCTCGATGCACCAATTTTATGTGCGGAAGGGGCGATTTTTTCCAGCAATCGCAAGGTATCTTCCGTTAGCGGTCGACGATCTCCAGTGTGCGGATCGGTGATGACGcc                                <  2:150201/148‑1 (MQ=255)
                                                                                                            tcaGACCGCTGACGACCTGGCGATGCAGGGCCTCTATCGCGCTCGATGCACCAATTTTATGTGCGGAAGGGGCGATTTTTTCCAGCAATCGCAAGGTATCTTCCGTTAGCGGTCGACGATCTCCAGTGTGCGGATCGGTGATGACGcc                                >  1:540930/1‑148 (MQ=255)
                                                                                                             caGACCGCTGACGACCTGGCGATGCAGGGCCTCTATCGCGCTCGATGCACCAATTTTATGTGCGGAAGGGGCGATTTTTTCCAGCAATCGCAAGGTATCTTCCGTTAGCGGTCGACGATCTCCAGTGTGCGGATCGGTGATGACGCCt                               >  1:3241139/1‑148 (MQ=255)
                                                                                                              aGACCGCTGACGACCTGGCGATGCAGGGCCTCTATCGCGCTCGATGCACCAATTTTATGTGCGGAAGGGGCGATTTTTTCCAGCAATCGCAAGGTATCTTCCGTTAGCGGTCGACGATCTCCAGTGTGCGGATCGGTGATGACGCCtt                              <  1:3052057/148‑1 (MQ=255)
                                                                                                                    cTGACGACCTGGCGATGCAGGGCCTCTATCGCGCTCGATGCACCAATTTTATGTGCGGAAGGGGCGATTTTTTCCAGCAATCGCAAGGTATCTTCCGTTAGCGGTCGACGATCTCCAGTGTGCGGATCGGTGATGACGCCTTCAAGcc                        >  2:2117271/1‑148 (MQ=255)
                                                                                                                    cTGACGACCTGGCGATGCAGGGCCTCTATCGCGCTCGATGCACCAATTTTATGTGCGGAAGGGGCGATTTTTTCCAGCAATCGCAAGGTATCTTCCGTTAGCGGTCGACGATCTCCAGTGTGCGGATCGGTGATGACGCCTTCAAGcc                        <  2:1753124/148‑1 (MQ=255)
                                                                                                                       acgacCTGGCGATGCAGGGCCTCTATCGCGCTCGATGCACCAATTTTATGTGCGGAAGGGGCGATTTTTTCCAGCAATCGCAAGGTATCTTCCGTTAGCGGTCGACGATCTCCAGTGTGCGGATCGGTGATGACGCCTTCAAGCCCAt                     <  2:775349/148‑1 (MQ=255)
                                                                                                                         gacCTGGCGATGCAGGGCCTCTATCGCGCTCGATGCACCAATTTTATGTGCGGAAGGGGCGATTTTTTCCAGCAATCGCAAGGTATCTTCCGTTAGCGGTCGACGATCTCCAGTGTGCGGATCGGTGATGACGCCTTCAAGCCCATAg                   <  2:1256673/148‑1 (MQ=255)
                                                                                                                         gacCTGGCGATGCAGGGCCTCTATCGCGCTCGATGCACCAATTTTATGTGCGGAAGGGGCGATTTTTTCCAGCAATCGCAAGGTATCTTCCGTTAGCGGTCGACGATCTCCAGTGTGCGGATCGGTGATGACGCCTTCAAGCCCATAg                   <  1:2437857/148‑1 (MQ=255)
                                                                                                                         gacCTGGCGATGCAGGGCCTCTATCGCGCTCGATGCACCAATTTTATGTGCGGAAGGGGCGATTTTTTCCAGCAATCGCAAGGTATCTTCCGTTAGCGGTCGACGATCTCCAGTGTGCGGATCGGTGATGACGCCTTCAAGCCCATAg                   <  1:2804337/148‑1 (MQ=255)
                                                                                                                            cTGGCGATGCAGGGCCTCTATCGCGCTCGATGCACCAATTTTATGTGCGGAAGGGGCGATTTTTTCCAGCAATCGCAAGGTATCTTCCGTTAGCGGTCGACGATCTCCAGTGTGCGGATCGGTGATGACGCCTTCAAGCCCATAGCGa                >  2:3395779/1‑148 (MQ=255)
                                                                                                                                 gATGCAGGGCCTCTCTCGCGCTCGATGCACCAATTTTATGTGCGGAAGGGGCGATTTTTTCCAGCAATCGCAAGGTATCTTCCGTTAGCGGTCGACGATCTCCAGTGTGCGGATCGGTGATGACGCCTTCAAGCCCATAGCGACAGGc           <  2:208823/148‑1 (MQ=255)
                                                                                                                                 gATGCAGGGCCTCTATCGCGCTCGATGCACCAATTTTATGTGCGGAAGGGGCGATTTTTTCCAGCAATCGCAAGGTATCTTCCGTTAGCGGTCGACGATCTCCAGTGTGCGGATCGGTGATGACGCCTTCAAGCCCATAGCGACAGGc           <  2:76610/148‑1 (MQ=255)
                                                                                                                                 gATGCAGGGCCTCTATCGCGCTCGATGCACCAATTTTATGTGCGGAAGGGGCGATTTTTTCCAGCAATCGCAAGGTATCTTCCGTTAGCGGTCGACGATCTCCAGTGTGCGGATCGGTGATGACGCCTTCAAGCCCATAGCGACAGGc           <  1:883338/148‑1 (MQ=255)
                                                                                                                                 gATGCAGGGCCTCTATCGCGCTCGATGCACCAATTTTATGTGCGGAAGGGGCGATTTTTTCCAGCAATCGCAAGGTATCTTCCGTTAGCGGTCGACGATCTCCAGTGTGCGGATCGGTGATGACGCCTTCAAGCCCATAGCGACAGGc           <  2:2444100/148‑1 (MQ=255)
                                                                                                                                       gggCCTCTATCGCGCTCGATGCACCAATTTTATGTGCGGAAGGGGCGATTTTTTCCAGCAATCGCAAGGTATCTTCCGTTAGCGGTCGACGATCTCCAGTGTGCGGATCGGTGATGACGCCTTCAAGCCCATAGCGACAGGCCTGGaa     >  1:2525541/1‑148 (MQ=255)
                                                                                                                                        ggCCTCTATCGCGCTCGATGCACCAATTTTATGTGCGGAAGGGGCGATTTTTTCCAGCAATCGCAAGGTATCTTCCGTTAGCGGTCGACGATCTCCAGTGTGCGGATCGGTGATGACGCCTTCAAGCCCATAGCGACAGGCCTGGaaa    <  1:1284487/148‑1 (MQ=255)
                                                                                                                                        ggCCTCTATCGCGCTCGATGCACCAATTTTATGTGCGGAAGGGGCGATTTTTTCCAGCAATCGCAAGGTATCTTCCGTTAGCGGTCGACGATCTCCAGTGTGCGGATCGGTGATGACGCCTTCAAGCCCATAGCGACAGGCCTGGaaa    <  1:439840/148‑1 (MQ=255)
                                                                                                                                        ggCCTCTATCGCGCTCGATGCACCAATTTTATGTGCGGAAGGGGCGATTTTTTCCAGCAATCGCAAGGTATCTTCCGTTAGCGGTCGACGATCTCCAGTGTGCGGATCGGTGATGACGCCTTCAAGCCCATAGCGACAGGCCTGGaaa    >  1:2886103/1‑148 (MQ=255)
                                                                                                                                        ggCCTCTATCGCGCTCGATGCACCAATTTTATGTGCGGAAGGGGCGATTTTTTCCAGCAATCGCAAGGTATCTTCCGTTAGCGGTCGACGATCTCCAGTGTGCGGATCGGTGATGACGCCTTCAAGCCCATAGCGACAGGCCTGGaaa    <  1:3095306/148‑1 (MQ=255)
                                                                                                                                          ccTCTATCGCGCTCGATGCACCAATTTTATGTGCGGAAGGGGCGATTTTTTCCAGCAATCGCAAGGTATCTTCCGTTAGCGGTCGACGATCTCCAGTGTGCGGATCGGTGATGACGCCTTCAAGCCCATAGCGACAGGCCTGGAAACg  <  2:2512066/148‑1 (MQ=255)
                                                                                                                                              |                                                                                                                                               
TAAGGCAATTCCCGGCTTAGTCACCGGCCCAGATCTCACAATGCTTTTTCACCAGCCCAATCAGCGAGCCGCCATCGGCGACGAAATCGCGCATTAGCTGCGCTTCATTCAGACCGCTGACGACCTGGCGATGCAGGGCCTCAATCGCGCTCGATGCACCAATTTTATGTGCGGAAGGGGCGATTTTTTCCAGCAATCGCAAGGTATCTTCCGTTAGCGGTCGACGATCTCCAGTGTGCGGATCGGTGATGACGCCTTCAAGCCCATAGCGACAGGCCTGGAAACG  >  CP009273/601705‑601990

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TCTTCTGTAAGGCAATTCCCGGCTTAGTCACCGGCCCAGATCTCACAATGCTTTTTCACCAGCCCAATCAGCGAGCCGCCATCGGCGACGAAATCGCGCATTAGCTGCGCTTCATTCAGACCGCTGACGACCTGGCGATGCAGGGCCTCAATCGCGCTCGATGCACCAATTTTATGTGCGGAAGGGGCGATTTTTTCCAGCAATCGCAAGGTATCTTCCGTTAGCGGTCGACGATCTCCAGTGTGCGGATCGGTGATGACGCCTTCAAGCCCATAGCGACAGGCCTGGAAACGGTTGA  >  CP009273/601698‑601995
                                                                                                                                                     |                                                                                                                                                    
TCTTCTGTAAGGCAATTCCCGGCTTAGTCACCGGCCCAGATCTCCCAATGCTTTTTCACCAGCCCAATCAGCGAGCCGCCATCGGCGACGAAATCGCGCATTAGCTGCGCTTCATTCAGACCGCTGACGACCTGGCGATGCAGGGCCTCT                                                                                                                                                      <  SRR19973065.1446583/150‑1 (MQ=60)
TCTTCTGTAAGGCAATTCCCGGCTTAGTCACCGGCCCAGATCTCACAATGCTTTTTCACCAGCCCAATCAGCGAGCCGCCATCGGCGACGAAATCGCGCATTAGCTGCGCTTCATTCAGACCGCTGACGACCTGGCGATGCAGGGCCTCT                                                                                                                                                      <  SRR19973065.1571077/150‑1 (MQ=60)
   TCTGTAAGGCAAGTCCCGGCTTAGTCACCGGCCCAGATCTCACAATGCTTTTTCACCAGCCCAATCAGCGAGCCGCCATCGGCGACGAAATCGCGCATTAGCTGCGCTTCATTCAGACCGCTGACGACCTGGCGATGCAGGGCCTCTATC                                                                                                                                                   <  SRR19973065.2066449/150‑1 (MQ=60)
     TGTAAGGCAATTCCCGGCTTAGTCACCGGCCCAGATCTCACAATGCTTTTTCACCAGCCCAATCAGCGAGCCGCCATCGGCGACGAAATCGCGCATTAGCTGCGCTTCATTCAGACCGCTGACGACCTGGCGATGCAGGGCCTCTATCGC                                                                                                                                                 >  SRR19973065.151464/1‑150 (MQ=60)
     TGTAAGGCAATTCCCGGCTTAGTCACCGGCCCAGATCTCACAATGCTTTTTCACCAGCCCAATCAGCGAGCCGCCATCGGCGACGAAATCGCGCATTAGCTGCGCTTCATTCAGACCGCTGACGACCTGGCGATGCAGGGCCTCTATCGC                                                                                                                                                 >  SRR19973065.3470750/1‑150 (MQ=60)
     TGTAAGGCAATTCCCGGCTTAGTCACCGGCCCAGATCTCACAATGCTTTTTCACCAGCCCAATCAGCGAGCCGCCATCGGCGACGAAATCGCGCATTAGCTGCGCTTCATTCAGACCGCTGACGACCTGGCGATGCAGGGCCTCTATCGC                                                                                                                                                 >  SRR19973065.1936742/1‑150 (MQ=60)
        AAGGCAATTCCCGGCTTAGTCACCGGCCCAGATCTCACAATGCTTTTTCACCAGCCCAATCAGCGAGCCGCCATCGGCGACGAAATCGCGCATTAGCTGCGCTTCATTCAGACCGCTGACGACCTGGCGATGCAGGGCCTCTATCGCGCT                                                                                                                                              >  SRR19973065.1919912/1‑150 (MQ=60)
         AGGCAATTCCCGGCTTAGTCACCGGCCCAGATCTCACAATGCTTTTTCACCAGCCCAATCAGCGAGCCGCCATCGGCGACGAAATCGCGCATTAGCTGCGCTTCATTCAGACCGCTGACGACCTGGCGATGCAGGGCCTCTATCGCGCTC                                                                                                                                             <  SRR19973065.312042/150‑1 (MQ=60)
          GGCAATTCCCGGCTTAGTCACCGGCCCAGATCTCACAATGCTTTTTCACCAGCCCAATCAGCGAGCCGCCATCGGCGACGAAATCGCGCATTAGCTGCGCTTCATTCAGACCGCTGACGACCTGGCGATGCAGGGCCTCTATCGCGCTCG                                                                                                                                            <  SRR19973065.200557/150‑1 (MQ=60)
          GGCAATTCCCGGCTTAGTCACCGGCCCAGATCTCACAATGCTTTTTCACCAGCCCAATCAGCGAGCCGCCATCGGCGACGAAATCGCGCATTAGCTGCGCTTCATTCAGACCGCTGACGACCTGGCGATGCAGGGCCTCTATCGCGCTCG                                                                                                                                            >  SRR19973065.3457785/1‑150 (MQ=60)
          GGCAATTCCCGGCTTAGTCACCGGCCCAGATCTCACAATGCTTTTTCACCAGCCCAATCAGCGAGCCGCCATCGGCGACGAAATCGCGCATTAGCTGCGCTTCATTCAGACCGCTGACGACCTGGCGATGCAGGGCCTCTATCGCGCTCG                                                                                                                                            <  SRR19973065.2688595/150‑1 (MQ=60)
            CAATTCCCGGCTTAGTCACCGGCCCAGATCTCACAATGCTTTTTCACCAGCCCAATCAGCGAGCCGCCATCGGCGACGAAATCGCGCATTAGCTGCGCTTCATTCAGACCGCTGACGACCTGGCGATGCAGGGCCTCTATCGCGCTCGAT                                                                                                                                          <  SRR19973065.3109576/150‑1 (MQ=60)
            CAATTCCCGGCTTAGTCACCGGCCCAGATCTCACAATGCTTTTTCACCAGCCCAATCAGCGAGCCGCCATCGGCGACGAAATCGCGCATTAGCTGCGCTTCATTCAGACCGCTGACGACCTGGCGATGCAGGGCCTCTATCGCGCTCGAT                                                                                                                                          <  SRR19973065.2632018/150‑1 (MQ=60)
            CAATTCCCGGCTTAGTCACCGGCCCAGATCTCACAATGCTTTTTCACCAGCCCAATCAGCGAGCCGCCATCGGCGACGAAATCGCGCATTAGCTGCGCTTCATTCAGACCGCTGACGACCTGGCGATGCAGGGCCTCTATCGCGCTCGAT                                                                                                                                          <  SRR19973065.1353550/150‑1 (MQ=60)
             AATTCCCGGCTTAGTCACCGGCCCAGATCTCACAATGCTTTTTCACCAGCCCAATCAGCGAGCCGCCATCGGCGACGAAATCGCGCATTAGCTGCGCTTCATTCAGACCGCTGACGACCTGGCGATGCAGGGCCTCTATCGCGCTCGATG                                                                                                                                         >  SRR19973065.3304883/1‑150 (MQ=60)
             AATTCCCGGCTTAGTCACCGGCCCAGATCTCACAATGCTTTTTCACCAGCCCAATCAGCGAGCCGCCATCGGCGACGAAATCGCGCATTAGCTGCGCTTCATTCAGACCGCTGACGACCTGGCGATGCAGGGCCTCTATCGCGCTCGATG                                                                                                                                         >  SRR19973065.3067167/1‑150 (MQ=60)
               TTCCCGGCTTAGTCACCGGCCCAGATCTCACAATGCTTTTTCACCAGCCCAATCAGCGAGCCGCCATCGGCGACGAAATCGCGCATTAGCTGCGCTTCATTCAGACCGCTGACGACCTGGCGATGCAGGGCCTCTATCGCGCTCGATGCA                                                                                                                                       <  SRR19973065.2888711/150‑1 (MQ=60)
               TTCCCGGCTTAGTCACCGGCCCAGATCTCACAATGCTTTTTCACCAGCCCAATCAGCGAGCCGCCATCGGCGACGAAATCGCGCATTAGCTGCGCTTCATTCAGACCGCTGACGACCTGGCGATGCAGGGCCTCTATCGCGCTCGATGCA                                                                                                                                       >  SRR19973065.1363499/1‑150 (MQ=60)
                 CCCGGCTTAGTCACCGGCCCAGATCTCACAATGCTTTTTCACCAGCCCAATCAGCGAGCCGCCATCGGCGACGAAATCGCGCATTAGCTGCGCTTCATTCAGACCGCTGACGACCTGGCGATGCAGGGCCTCTATCGCGCTCGATGCACC                                                                                                                                     >  SRR19973065.1147626/1‑150 (MQ=60)
                 CCCGGCTTAGTCACCGGCCCAGATCTCACAATGCTTTTTCACCAGCCCAATCAGCGAGCCGCCATCGGCGACGAAATCGCGCATTAGCTGCGCTTCATTCAGACCGCTGACGACCTGGCGATGCAGGGCCTCTATCGCGCTCGATGCACC                                                                                                                                     >  SRR19973065.2910435/1‑150 (MQ=60)
                  CCGGCTTAGTCACCGGCCCAGATCTCACAATGCTTTTTCACCAGCCCAATCAGCGAGCCGCCATCGGCGACGAAATCGCGCATTAGCTGCGCTTCATTCAGACCGCTGACGACCTGGCGATGCAGGGCCTCTATCGCGCTCGATGCACCA                                                                                                                                    <  SRR19973065.757223/150‑1 (MQ=60)
                  CCGGCTTAGTCACCGGCCCAGATCTCACAATGCTTTTTCACCAGCCCAATCAGCGAGCCGCCATCGGCGACGAAATCGCGCATTAGCTGCGCTTCATTCAGACCGCTGACGACCTGGCGATGCAGGGCCTCTATCGCGCTCGATGCACCA                                                                                                                                    >  SRR19973065.780792/1‑150 (MQ=60)
                  CCGGCTTAGTCACCGGCCCAGATCTCACAATGCTTTTTCACCAGCCCAATCAGCGAGCCGCCATCGGCGACGAAATCGCGCATTAGCTGCGCTTCATTCAGACCGCTGACGACCTGGCGATGCAGGGCCTCTATCGCGCTCGATGCACCA                                                                                                                                    <  SRR19973065.756148/150‑1 (MQ=60)
                    GGCTTAGTCACCGGCCCAGATCTCACAATGCTTTTTCACCAGCCCAATCAGCGAGCCGCCATCGGCGACGAAATCGCGCATTAGCTGCGCTTCATTCAGACCGCTGACGACCTGGCGATGCAGGGCCTCTATCGCGCTCGATGCACCAAT                                                                                                                                  <  SRR19973065.3179012/150‑1 (MQ=60)
                        TAGTCACCGGCCCAGATCTCACAATGCTTTTTCACCAGCCCAATCAGCGAGCCGCCATCGGCGACGAAATCGCGCATTAGCTGCGCTTCATTCAGACCGCTGACGACCTGGCGATGCAGGGCCTCTATCGCGCTCGATGCACCAATTTTA                                                                                                                              >  SRR19973065.780501/1‑150 (MQ=60)
                         AGTCACCGGCCCAGATCTCACAATGCTTTTTCACCAGCCCAATCAGCGAGCCGCCATCGGCGACGAAATCGCGCATTAGCTGCGCTTCATTCAGACCGCTGACGACCTGGCGATGCAGGGCCTCTATCGCGCTCGATGCACCAATTTTAT                                                                                                                             <  SRR19973065.3036131/150‑1 (MQ=60)
                          GTCACCGGCCCAGATCTCACAATGCTTTTTCACCAGCCCAATCAGCGAGCCGCCATCGGCGACGAAATCGCGCATTAGCTGCGCTTCATTCAGACCGCTGACGACCTGGCGATGCAGGGCCTCTATCGCGCTCGATGCACCAATTTTATG                                                                                                                            >  SRR19973065.1645375/1‑150 (MQ=60)
                          GTCACCGGCCCAGATCTCACAATGCTTTTTCACCAGCCCAATCAGCGAGCCGCCATCGGCGACGAAATCGCGCATTAGCTGCGCTTCATTCAGACCGCTGACGACCTGGCGATGCAGGGCCTCTATCGCGCTCGATGCACCAATTTTATG                                                                                                                            >  SRR19973065.3394870/1‑150 (MQ=60)
                          GTCACCGGCCCAGATCTCACAATGCTTTTTCACCAGCCCAATCAGCGAGCCGCCATCGGCGACGAAATCGCGCATTAGCTGCGCTTCATTCAGACCGCTGACGACCTGGCGATGCAGGGCCTCTATCGCGCTCGATGCACCAATTTTATG                                                                                                                            >  SRR19973065.3394855/1‑150 (MQ=60)
                            CACCGGCCCAGATCTCACAATGCTTTTTCACCAGCCCAATCAGCGAGCCGCCATCGGCGACGAAATCGCGCATTAGCTGCGCTTCATTCAGACCGCTGACGACCTGGCGATGCAGGGCCTCTATCGCGCTCGATGCACCAATTTTATGTG                                                                                                                          <  SRR19973065.98237/150‑1 (MQ=60)
                            CACCGGCCCAGATCTCACAATGCTTTTTCACCAGCCCAATCAGCGAGCCGCCATCGGCGACGAAATCGCGCATTAGCTGCGCTTCATTCAGACCGCTGACGACCTGGCGATGCAGGGCCTCTATCGCGCTCGATGCACCAATTTTATGTG                                                                                                                          <  SRR19973065.98406/150‑1 (MQ=60)
                            CACCGGCCCAGATCTCACAATGCTTTTTCACCAGCCCAATCAGCGAGCCGCCATCGGCGACGAAATCGCGCATTAGCTGCGCTTCATTCAGACCGCTGACGACCTGGCGATGCAGGGCCTCTATCGCGCTCGATGCACCAATTTTATGTG                                                                                                                          >  SRR19973065.1181985/1‑150 (MQ=60)
                                GGCCCAGATCTCACAATGCTTTTTCACCAGCCCAATCAGCGAGCCGCCATCGGCGACGAAATCGCGCATTAGCTGCGCTTCATTCAGACCGCTGACGACCTGGCGATGCAGGGCCTCTATCGCGCTCGATGCACCAATTTTATGTGCGGA                                                                                                                      >  SRR19973065.1044284/1‑150 (MQ=60)
                                       ATCTCACAATGCTTTTTCACCAGCCCAATCAGCGAGCCGCCATCGGCGACGAAATCGCGCATTAGCTGCGCTTCATTCAGACCGCTGACGACCTGGCGATGCAGGGCCTCTATCGCGCTCGATGCACCAATTTTATGTGCGGAAGGGGCG                                                                                                               >  SRR19973065.2977400/1‑150 (MQ=60)
                                       ATCTCACAATGCTTTTTCACCAGCCCAATCAGCGAGCCGCCATCGGCGACGAAATCGCGCATTAGCTGCGCTTCATTCAGACCGCTGACGACCTGGCGATGCAGGGCCTCTATCGCGCTCGATGCACCAATTTTATGTGCGGAAGGGGCG                                                                                                               >  SRR19973065.639758/1‑150 (MQ=60)
                                        TCTCACAATGCTTTTTCACCAGCCCAATCAGCGAGCCGCCATCGGCGACGAAATCGCGCATTAGCTGCGCTTCATTCAGACCGCTGACGACCTGGCGATGCAGGGCCTCTATCGCGCTCGATGCACCAATTTTATGTGCGGAAGGGGCGA                                                                                                              <  SRR19973065.448204/150‑1 (MQ=60)
                                             CAATGCTTTTTCACCAGCCCAATCAGCGAGCCGCCATCGGCGACGAAATCGCGCATTAGCTGCGCTTCATTCAGACCGCTGACGACCTGGCGATGCAGGGCCTCTATCGCGCTCGATGCACCAATTTTATGTGCGGAAGGGGCGATTTTT                                                                                                         <  SRR19973065.317095/150‑1 (MQ=60)
                                             CAATGCTTTTTCACCAGCCCAATCAGCGAGCCGCCATCGGCGACGAAATCGCGCATTAGCTGCGCTTCATTCAGACCGCTGACGACCTGGCGATGCAGGGCCTCTATCGCGCTCGATGCACCAATTTTATGTGCGGAAGGGGCGATTTTT                                                                                                         <  SRR19973065.643594/150‑1 (MQ=60)
                                              AATGCTTTTTCACCAGCCCAATCAGCGAGCCGCCATCGGCGACGAAATCGCGCATTAGCTGCGCTTCATTCAGACCGCTGACGACCTGGCGATGCAGGGCCTCTATCGCGCTCGATGCACCAATTTTATGTGCGGAAGGGGCGATTTTTT                                                                                                        >  SRR19973065.1226044/1‑150 (MQ=60)
                                                  CTTTTTCACCAGCCCAATCAGCGAGCCGCCATCGGCGACGAAATCGCGCATTAGCTGCGCTTCATTCAGACCGCTGACGACCTGGCGATGCAGGGCCTCTATCGCGCTCGATGCACCAATTTTATGTGCGGAAGGGGCGATTTTTTCCAG                                                                                                    <  SRR19973065.3052716/150‑1 (MQ=60)
                                                    TTTTCACCAGCCCAATCAGCGAGCCGCCATCGGCGACGAAATCGCGCATTAGCTGCGCTTCATTCAGACCGCTGACGACCTGGCGATGCAGGGCCTCTATCGCGCTCGATGCACCAATTTTATGTGCGGAAGGGGCGATTTTTTCCAGCA                                                                                                  >  SRR19973065.3231898/1‑150 (MQ=60)
                                                    TTTTCACCAGCCCAATCAGCGAGCCGCCATCGGCGACGAAATCGCGCATTAGCTGCGCTTCATTCAGACCGCTGACGACCTGGCGATGCAGGGCCTCAATCGCGCTCGATGCACCAATTTTATGTGCGGAAGGGGCGATTTTTTCCAGCA                                                                                                  <  SRR19973065.3123410/150‑1 (MQ=60)
                                                        CACCAGCCCAATCAGCGAGCCGCCATCGGCGACGAAATCGCGCATTAGCTGCGCTTCATTCAGACCGCTGACGACCTGGCGATGCAGGGCCTCTATCGCGCTCGATGCACCAATTTTATGTGCGGAAGGGGCGATTTTTTCCAGCAATCG                                                                                              <  SRR19973065.1628309/150‑1 (MQ=60)
                                                           CAGCCCAATCAGCGAGCCGCCATCGGCGACGAAATCGCGCATTAGCTGCGCTTCATTCAGACCGCTGACGACCTGGCGATGCAGGGCCTCTATCGCGCTCGATGCACCAATTTTATGTGCGGAAGGGGCGATTTTTTCCAGCAATCGCAA                                                                                           <  SRR19973065.2642574/150‑1 (MQ=60)
                                                               CCAATCAGCGAGCCGCCATCGGCGACGAAATAGCGCATTAGCTGCGCTTCATTCAGACCGCTGACGACCTGGCGATGCAGGGCCTCTATCGCGCTCGATGCACCAATTTTATGTGCGGAAGGGGCGATTTTTTCCAGCAATCGCAAGGTA                                                                                       >  SRR19973065.1198837/1‑150 (MQ=60)
                                                                CAATCAGCGAGCCGCCATCGGCGACGAAATCGCGCATTAGCTGCGCTTCATTCAGACCGCTGACGACCTGGCGATGCAGGGCCTCTATCGCGCTCGATGCACCAATTTTATGTGCGGAAGGGGCGATTTTTTCCAGCAATCGCAAGGTAT                                                                                      >  SRR19973065.2629379/1‑150 (MQ=60)
                                                                 AATCAGCGAGCCGCCATCGGCGACGAAATCGCGCATTAGCTGCGCTTCATTCAGACCGCTGACGACCTGGCGATGCAGGGCCTCTATCGCGCTCGATGCACCAATTTTATGTGCGGAAGGGGCGATTTTTTCCAGCAATCGCAAGGTATC                                                                                     >  SRR19973065.358610/1‑150 (MQ=60)
                                                                 AATCAGCGAGCCGCCATCGGCGACGAAATCGCGCATTAGCTGCGCTTCATTCAGACCGCTGACGACCTGGCGATGCAGGGCCTCTATCGCGCTCGATGCACCAATTTTATGTGCGGAAGGGGCGATTTTTTCCAGCAATCGCAAGGTATC                                                                                     >  SRR19973065.358671/1‑150 (MQ=60)
                                                                   TCAGCGAGCCGCCATCGGCGACGAAATCGCGCATTAGCTGCGCTTCATTCAGACCGCTGACGACCTGGCGATGCAGGGCCTCTATCGCGCTCGATGCACCAATTTTATGTGCGGAAGGGGCGATTTTTTCCAGCAATCGCAAGGTATCTT                                                                                   <  SRR19973065.391740/150‑1 (MQ=60)
                                                                   TCAGCGAGCCGCCATCGGCGACGAAATCGCGCATTAGCTGCGCTTCATTCAGACCGCTGACGACCTGGCGATGCAGGGCCTCTATCGCGCTCGATGCACCAATTTTATGTGCGGAAGGGGCGATTTTTTCCAGCAATCGCAAGGTATCTT                                                                                   <  SRR19973065.1040323/150‑1 (MQ=60)
                                                                   TCAGCGAGCCGCCATCGGCGACGAAATCGCGCATTAGCTGCGCTTCATTCAGACCGCTGACGACCTGGCGATGCAGGGCCTCTATCGCGCTCGATGCACCAATTTTATGTGCGGAAGGGGCGATTTTTTCCAGCAATCGCAAGGTATCTT                                                                                   <  SRR19973065.197616/150‑1 (MQ=60)
                                                                     AGCGAGCCGCCATCGGCGACGAAATCGCGCATTAGCTGCGCTTCATTCAGACCGCTGACGACCTGGCGATGCAGGGCCTCTATCGCGCTCGATGCACCAATTTTATGTGCGGAAGGGGCGATTTTTTCCAGCAATCGCAAGGTATCTTCC                                                                                 >  SRR19973065.3294445/1‑150 (MQ=60)
                                                                     AGCGAGCCGCCATCGGCGACGAAATCGCGCATTAGCTGCGCTTCATTCAGACCGCTGACGACCTGGCGATGCAGGGCCTCTATCGCGCTCGATGCACCAATTTTATGTGCGGAAGGGGCGATTTTTTCCAGCAATCGCAAGGTATCTTCC                                                                                 <  SRR19973065.2488224/150‑1 (MQ=60)
                                                                     AGCGAGCCGCCATCGGCGACGAAATCGCGCATTAGCTGCGCTTCATTCAGACCGCTGACGACCTGGCGATGCAGGGCCTCTATCGCGCTCGATGCACCAATTTTAGGTGCGGAAGGGGCGATTTTTTCCAGCAATCGCAAGGTATCTTCC                                                                                 >  SRR19973065.3294067/1‑150 (MQ=60)
                                                                      GCGAGCCGCCATCGGCGACGAAATCGCGCATTAGCTGCGCTTCATTCAGACCGCTGACGACCTGGCGATGCAGGGCCTCTATCGCGCTCGATGCACCAATTTTATGTGCGGAAGGGGCGATTTTTTCCAGCAATCGCAAGGTATCTTCCG                                                                                <  SRR19973065.3366040/150‑1 (MQ=60)
                                                                       CGAGCCGCCATCGGCGACGAAATCGCGCATTAGCTGCGCTTCATTCAGACCGCTGACGACCTGGCGATGCAGGGCCTCTATCGCGCTCGATGCACCAATTTTATGTGCGGAAGGGGCGATTTTTTCCAGCAATCGCAAGGTATCTTCCGT                                                                               <  SRR19973065.1156387/150‑1 (MQ=60)
                                                                       CGAGCCGCCATCGGCGACGAAATCGCGCATTAGCTGCGCTTCATTCAGACCGCTGACGACCTGGCGATGCAGGGCCTCTATCGCGCTCGATGCACCAATTTTATGTGCGGAAGGGGCGATTTTTTCCAGCAATCGCAAGGTATCTTCCGT                                                                               <  SRR19973065.1156461/150‑1 (MQ=60)
                                                                        GAGCCGCCATCGGCGACGAAATCGCGCATTAGCTGCGCTTCATTCAGACCGCTGACGACCTGGCGATGCAGGGCCTCTATCGCGCTCGATGCACCAATTTTATGTGCGGAAGGGGCGATTTTTTCCAGCAATCGCAAGGTATCTTCCGTT                                                                              >  SRR19973065.2059119/1‑150 (MQ=60)
                                                                        GAGCCGCCATCGGCGACGAAATCGCGCATTAGCTGCGCTTCATTCAGACCGCTGACGACCTGGCGATGCAGGGCCTCTATCGCGCTCGATGCACCAATTTTATGTGCGGAAGGGGCGATTTTTTCCAGCAATCGCAAGGTATCTTCCGTT                                                                              >  SRR19973065.267010/1‑150 (MQ=60)
                                                                            CGCCATCGGCGACGAAATCGCGCATTAGCTGCGCTTCATTCAGACCGCTGACGACCTGGCGATGCAGGGCCTCTATCGCGCTCGATGCACCAATTTTATGTGCGGAAGGGGCGATTTTTTCCAGCAATCGCAAGGTATCTTCCGTTAGCG                                                                          >  SRR19973065.941890/1‑150 (MQ=60)
                                                                            CGCCATCGGCGACGAAATCGCGCATTAGCTGCGCTTCATTCAGACCGCTGACGACCTGGCGATGCAGGGCCTCTATCGCGCTCGATGCACCAATTTTATGTGCGGAAGGGGCGATTTTTTCCAGCAATCGCAAGGTATCTTCCGTTAGCG                                                                          >  SRR19973065.3036906/1‑150 (MQ=60)
                                                                                 TCGGCGACGAAATCGCGCATTAGCTGCGCTTCATTCAGACCGCTGACGACCTGGCGATGCAGGGCCTCTATCGCGCTCGATGCACCAATTTTATGTGCGGAAGGGGCGATTTTTTCCAGCAATCGCAAGGTATCTTCCGTTAGCGGTCGA                                                                     >  SRR19973065.729518/1‑150 (MQ=60)
                                                                                       ACGAAATCGCGCATTAGCTGCGCTTCATTCAGACCGCTGACGACCTGGCGATGCAGGGCCTCTATCGCGCTCGATGCACCAATTTTATGTGCGGAAGGGGCGATTTTTTCCAGCAATCGCAAGGTATCTTCCGTTAGCGGTCGACGATCT                                                               <  SRR19973065.500042/150‑1 (MQ=60)
                                                                                       ACGAAATCGCGCATTAGCTGCGCTTCATTCAGACCGCTGACGACCTGGCGATGCAGGGCCTCTATCGCGCTCGATGCACCAATTTTATGTGCGGAAGGGGCGATTTTTTCCAGCAATCGCAAGGTATCTTCCGTTAGCGGTCGACGATCT                                                               <  SRR19973065.499886/150‑1 (MQ=60)
                                                                                        CGAAATCGCGCATTAGCTGCGCTTCATTCAGACCGCTGACGACCTGGCGATGCAGGGCCTCTATCGCGCTCGATGCACCAATTTTATGTGCGGAAGGGGCGATTTTTTCCAGCAATCGCAAGGTATCTTCCGTTAGCGGTCGACGATCTC                                                              >  SRR19973065.775184/1‑150 (MQ=60)
                                                                                          AAATCGCGCATTAGCTGCGCTTCATTCAGACCGCTGACGACCTGGCGATGCAGGGCCTCTATCGCGCTCGATGCACCAATTTTATGTGCGGAAGGGGCGATTTTTTCCAGCAATCGCAAGGTATCTTCCGTTAGCGGTCGACGATCTCCA                                                            <  SRR19973065.1630692/150‑1 (MQ=60)
                                                                                          AAATCGCGCATTAGCTGCGCTTCATTCAGACCGCTGACGACCTGGCGATGCAGGGCCTCTATCGCGCTCGATGCACCAATTTTATGTGCGGAAGGGGCGATTTTTTCCAGCAATCGCAAGGTATCTTCCGTTAGCGGTCGACGATCTCCA                                                            <  SRR19973065.1630715/150‑1 (MQ=60)
                                                                                          AAATCGCGCATTAGCTGCGCTTCATTCAGACCGCTGACGACCTGGCGATGCAGGGCCTCTATCGCGCTCGATGCACCAATTTTATGTGCGGAAGGGGCGATTTTTTCCAGCAATCGCAAGGTATCTTCCGTTAGCGGTCGACGATCTCCA                                                            >  SRR19973065.3532463/1‑150 (MQ=60)
                                                                                          AAATCGCGCATTAGCTGCGCTTCATTCAGACCGCTGACGACCTGGCGATGCAGGGCCTCTATCGCGCTCGATGCACCAATTTTATGTGCGGAAGGGGCGATTTTTTCCAGCAATCGCAAGGTATCTTCCGTTAGCGGTCGACGATCTCCA                                                            >  SRR19973065.2573639/1‑150 (MQ=60)
                                                                                           AATCGCGCATTAGCTGCGCTTCATTCAGACCGCTGACGACCTGGCGATGCAGGGCCTCTATCGCGCTCGATGCACCAATTTTATGTGCGGAAGGGGCGATTTTTTCCAGCAATCGCAAGGTATCTTCCGTTAGCGGTCGACGATCTCCAG                                                           <  SRR19973065.3027012/150‑1 (MQ=60)
                                                                                           AATCGCGCATTAGCTGCGCTTCATTCAGACCGCTGACGACCTGGCGATGCAGGGCCTCTATCGCGCTCGATGCACCAATTTTATGTGCGGAAGGGGCGATTTTTTCCAGCAATCGCAAGGTATCTTCCGTTAGCGGTCGACGATCTCCAG                                                           <  SRR19973065.3026607/150‑1 (MQ=60)
                                                                                            ATCGCGCATTAGCTGCGCTTCATTCAGACCGCTGACGACCTGGCGATGCAGGGCCTCTATCGCGCTCGATGCACCAATTTTATGTGCGGAAGGGGCGATTTTTTCCAGCAATCGCAAGGTATCTTCCGTTAGCGGTCGACGATCTCCAGT                                                          <  SRR19973065.1009198/150‑1 (MQ=60)
                                                                                                 GCATTAGCTGCGCTTCATTCAGACCGCTGACGACCTGGCGATGCAGGGCCTCTATCGCGCTCGATGCACCAATTTTATGTGCGGAAGGGGCGATTTTTTCCAGCAATCGCAAGGTATCTTCCGTTAGCGGTCGACGATCTCCAGTGTGCG                                                     <  SRR19973065.2881952/150‑1 (MQ=60)
                                                                                                 GCATTAGCTGCGCTTCATTCAGACCGCTGACGACCTGGCGATGCAGGGCCTCTATCGCGCTCGATGCACCAATTTTATGTGCGGAAGGGGCGATTTTTTCCAGCAATCGCAAGGTATCTTCCGTTAGCGGTCGACGATCTCCAGTGTGCG                                                     >  SRR19973065.431169/1‑150 (MQ=60)
                                                                                                 GCATTAGCTGCGCTTCATTCAGACCGCTGACGACCTGGCGATGCAGGGCCTCTATCGCGCTCGATGCACCAATTTTATGTGCGGAAGGGGCGATTTTTTCCAGCAATCGCAAGGTATCTTCCGTTAGCGGTCGACGATCTCCAGTGTGCG                                                     <  SRR19973065.831974/150‑1 (MQ=60)
                                                                                                 GCATTAGCTGCGCTTCATTCAGACCGCTGACGACCTGGCGATGCAGGGCCTCTATCGCGCTCGATGCACCAATTTTATGTGCGGAAGGGGCGATTTTTTCCAGCAATCGCAAGGTATCTTCCGTTAGCGGTCGACGATCTCCAGTGTGCG                                                     <  SRR19973065.2602572/150‑1 (MQ=60)
                                                                                                  CATTAGCTGCGCTTCATTCAGACCGCTGACGACCTGGCGATGCAGGGCCTCTATCGCGCTCGATGCACCAATTTTATGTGCGGAAGGGGCGATTTTTTCCAGCAATCGCAAGGTATCTTCCGTTAGCGGTCGACGATCTCCAGTGTGCGG                                                    <  SRR19973065.1221700/150‑1 (MQ=60)
                                                                                                  CATTAGCTGCGCTTCATTCAGACCGCTGACGACCTGGCGATGCAGGGCCTCTATCGCGCTCGATGCACCAATTTTATGTGCGGAAGGGGCGATTTTTTCCAGCAATCGCAAGGTATCTTCAGTTAGCGGTCGACGATCTCCAGTGTGCGG                                                    >  SRR19973065.636023/1‑150 (MQ=60)
                                                                                                     TAGCTGCGCTTCATTCAGACCGCTGACGACCTGGCGATGCAGGGCCTCTATCGCGCTCGATGCACCAATTTTATGTGCGGAAGGGGCGATTTTTTCCAGCAATCGCAAGGTATCTTCCGTTAGCGGTCGACGATCTCCAGTGTGCGGATC                                                 <  SRR19973065.766181/150‑1 (MQ=60)
                                                                                                      AGCTGCGCTTCATTCAGACCGCTGACGACCTGGCGATGCAGGGCCTCTATCGCGCTCGATGCACCAATTTTATGTGCGGAAGGGGCGATTTTTTCCAGCAATCGCAAGGTATCTTCCGTTAGCGGTCGACGATCTCCAGTGTGCGGATCG                                                >  SRR19973065.735760/1‑150 (MQ=60)
                                                                                                      AGCTGCGCTTCATTCAGACCGCTGACGACCTGGCGATGCAGGGCCTCTATCGCGCTCGATGCACCAATTTTATGTGCGGAAGGGGCGATTTTTTCCAGCAATCGCAAGGTATCTTCCGTTAGCGGTCGACGATCTCCAGTGTGCGGATCG                                                >  SRR19973065.3460636/1‑150 (MQ=60)
                                                                                                      AGCTGCGCTTCATTCAGACCGCTGACGACCTGGCGATGCAGGGCCTCTATCGCGCTCGATGCACCAATTTTATGTGCGGAAGGGGCGATTTTTTCCAGCAATCGCAAGGTATCTTCCGTTAGCGGTCGACGATCTCCAGTGTGCGGATCG                                                >  SRR19973065.3461578/1‑150 (MQ=60)
                                                                                                          GCGCTTCATTCAGACCGCTGACGACCTGGCGATGCAGGGCCTCTATCGCGCTCGATGCACCAATTTTATGTGCGGAAGGGGCGATTTTTTCCAGCAATCGCAAGGTATCTTCCGTTAGCGGTCGACGATCTCCAGTGTGCGGATCGGTGA                                            <  SRR19973065.1040849/150‑1 (MQ=60)
                                                                                                             CTTCATTCAGACCGCTGACGACCTGGCGATGCAGGGCCTCTATCGCGCTCGATGCACCAATTTTATGTGCGGAAGGGGCGATTTTTTCCAGCAATCGCAAGGTATCGTCCGTTAGCGGTCGACGATCTCCAGTGTGCGGATCGGTGATGA                                         >  SRR19973065.2290413/1‑150 (MQ=60)
                                                                                                              TTCATTCAGACCGCTGACGACCTGGCGATGCAGGGCCTCTATCGCGCTCGATGCACCAATTTTATGTGCGGAAGTGGAGATTTTTTCCAGCAATCGCAATGTATCTTGCGTTAGCGGTCGACTATCTCCAGTGTGCGGATCGGTGATGAC                                        <  SRR19973065.189625/150‑1 (MQ=60)
                                                                                                                 ATTCAGACCGCTGACGACCTGGCGATGCAGGGCCTCTATCGCGCTCGATGCACCAATTTTATGTGCGGAAGGGGCGATTTTTTCCAGCAATCGCAAGGTATCTTCCGTTAGCGGTCGACGATCTCCAGTGTGCGGATCGGTGATGACGCC                                     >  SRR19973065.544442/1‑150 (MQ=60)
                                                                                                                  TTCAGACCGCTGACGACCTGGCGATGCAGGGCCTCTATCGCGCTCGATGCACCAATTTTATGTGCGGAAGGGGCGATTTTTTCCAGCAATCGCAAGGTATCTTCCGTTAGCGGTCGACGATCTCCAGTGTGCGGATCGGTGATGACGCCT                                    >  SRR19973065.3260671/1‑150 (MQ=60)
                                                                                                                   TCAGACCGCTGACGACCTGGCGATGCAGGGCCTCTATCGCGCTCGATGCACCAATTTTATGTGCGGAAGGGGCGATTTTTTCCAGCAATCGCAAGGTATCTTCCGTTAGCGGTCGACGATCTCCAGTGTGCGGATCGGTGATGACGCCTT                                   <  SRR19973065.1089260/150‑1 (MQ=60)
                                                                                                                     AGACCGCTGACGACCTGGCGATGCAGGGCCTCTATCGCGCTCGATGCACCAATTTTATGTGCGGAAGGGGCGATTTTTTCCAGCAATCGCAAGGTATCTTCCGTTAGCGGTCGACGATCTCCAGTGTGCGGATCGGTGATGACGCCTTCA                                 <  SRR19973065.3070695/150‑1 (MQ=60)
                                                                                                                     AGACCGCTGACGACCTGGCGATGCAGGGCCTCTATCGCGCTCGATGCACCAATTTTATGTGCGGAAGGGGCGATTTTTTCCAGCAATCGCAAGGTATCTTCCGTTAGCGGTCGACGATCTCCAGTGTGCGGATCGGTGATGACGCCTTCA                                 <  SRR19973065.3070719/150‑1 (MQ=60)
                                                                                                                     AGACCGCTGACGACCGGGCGATGCAGGGCCTCTATCGCGCTCGATGCACCAATTTTATGTGCGGAAGGGGCGATTTTTTCCAGCAATCGCAAGGTATCTTCCGTTAGCGGTCGACGATCTCCAGTGTGCGGATCGGTGATGACGCCTTCA                                 <  SRR19973065.3070760/150‑1 (MQ=60)
                                                                                                                         CGCTGACGACCTGGCGATGCAGGGCCTCTATCGCGCTCGATGCACCAATTTTATGTGCGGAAGGGGCGATTTTTTCCAGCAATCGCAAGGTATCTTCCGTTAGCGGTCGACGATCTCCAGTGTGCGGATCGGTGATGACGCCTTCAAGCC                             <  SRR19973065.2878311/150‑1 (MQ=60)
                                                                                                                           CTGACGACCTGGCGATGCAGGGCCTCTATCGCGCTCGATGCACCAATTTTATGTGCGGAAGGGGCGATTTTTTCCAGCAATCGCAAGGTATCTTCCGTTAGCGGTCGACGATCTCCAGTGTGCGGATCGGTGATGACGCCTTCAAGCCCA                           <  SRR19973065.469228/150‑1 (MQ=60)
                                                                                                                            TGACGACCTGGCGATGCAGGGCCTCTATCGCGCTCGATGCACCAATTTTATGTGCGGAAGGGGCGATTTTTTCCAGCAATCGCAAGGTATCTTCCGTTAGCGGTCGACGATCTCCAGTGTGCGGATCGGTGATGACGCCTTCAAGCCCAT                          >  SRR19973065.2819030/1‑150 (MQ=60)
                                                                                                                              ACGACCTGGCGATGCAGGGCCTCTATCGCGCTCGATGCACCAATTTTATGTGCGGAAGGGGCGATTTTTTCCAGCAATCGCAAGGTATCTTCCGTTAGCGGTCGACGATCTCCAGTGTGCGGATCGGTGATGACGCCTTCAAGCCCATAG                        <  SRR19973065.680847/150‑1 (MQ=60)
                                                                                                                                GACCTGGCGATGCAGGGCCTCTATCGCGCTCGATGCACCAATTTTATGTGCGGAAGGGGCGATTTTTTCCAGCAATCGCAAGGTATCTTCCGTTAGCGGTCGACGATCTCCAGTGTGCGGATCGGTGATGACGCCTTCAAGCCCATAGCG                      <  SRR19973065.2821696/150‑1 (MQ=60)
                                                                                                                                GACCTGGCGATGCAGGGCCTCTATCGCGCTCGATGCACCAATTTTATGTGCGGAAGGGGCGATTTTTTCCAGCAATCGCAAGGTATCTTCCGTTAGCGGTCGACGATCTCCAGTGTGCGGATCGGTGATGACGCCTTCAAGCCCATAGCG                      <  SRR19973065.2453230/150‑1 (MQ=60)
                                                                                                                                  CCTGGCGATTCAGGGCCTCTATCGCGCTCGATGCACCAATTTTATGTGCGGAAGGGGCGATTTTTTCCAGCAATCGCAAGGTATCTTCCGTTAGCGGTCGACGATCTCCAGTGTGCGGATCGGTGATGACGCCTTCAAGCCCATAGCGAC                    <  SRR19973065.2712367/150‑1 (MQ=60)
                                                                                                                                    TGGCGATGCAGGGCCTCTATCGCGCTCGATGCACCAATTTTATGTGCGGAAGGGGCGATTTTTTCCAGCAATCGCAAGGTATCTTCCGTTAGCGGTCGACGATCTCCAGTGTGCGGATCGGTGATGACGCCTTCAAGCCCATAGCGACAG                  >  SRR19973065.2481893/1‑150 (MQ=60)
                                                                                                                                        GATGCAGGGCCTCTATCGCGCTCGATGCACCAATTTTATGTGCGGAAGGGGCGATTTTTTCCAGCAATCGCAAGGTATCTTCCGTTAGCGGTCGACGATCTCCAGTGTGCGGATCGGTGATGACGCCTTCAAGCCCATAGCGACAGGCCT              <  SRR19973065.888957/150‑1 (MQ=60)
                                                                                                                                            CAGGGCCTCTATCGCGCTCGATGCACCAATTTTATGTGCGGAAGGGGCGATTTTTTCCAGCAATCGCAAGGTATCTTCCGTTAGCGGTCGACGATCTCCAGTGTGCGGATCGGTGATGACGCCTTCAAGCCCATAGCGACAGGCCTGGAA          >  SRR19973065.2541396/1‑150 (MQ=60)
                                                                                                                                             AGGGCCTCTATCGCGCTCGATGCACCAATTTTATGTGCGGAAGGGGCGATTTTTTCCAGCAATCGCAATGTATCTTCCGTTAGCGGTCGACGATCTCCAGTGTGCGGATCGGTGATGACGCCTTCAAGCCCCTAGCGACAGGCCTGGAAA         >  SRR19973065.1320069/1‑150 (MQ=60)
                                                                                                                                             AGGGCCTCTATCGCGCTCGATGCACCAATTTTATGTGCGGAAGGGGCGATTTTTTCCAGCAATCGCAAGGTATCTTCCGTTAGCGGTCGACGATCTCCAGTGTGCGGATCGGTGATGACGCCTTCAAGCCCATAGCGACAGGCCTGGAAA         >  SRR19973065.2903753/1‑150 (MQ=60)
                                                                                                                                              GGGCCTCTATCGCGCTCGATGCACCAATTTTATGTGCGGAAGGGGCGATTTTTTCCAGCAATCGCAAGGTATCTTCCGTTAGCGGTCGACGATCTCCAGTGTGCGGATCGGTGATGACGCCTTCAAGCCCATAGCGACAGGCCTGGAAAC        <  SRR19973065.634119/150‑1 (MQ=60)
                                                                                                                                               GGCCTCTATCGCGCTCGATGCACCAATTTTATGTGCGGAAGGGGCGATTTTTTCCAGCAATCGCAAGGTATCTTCCGTTAGCGGTCGACGATCTCCAGTGTGCGGATCGGTGATGACGCCTTCAAGCCCATAGCGACAGGCCTGGAAACG       <  SRR19973065.1292396/150‑1 (MQ=60)
                                                                                                                                               GGCCTCTATCGCGCTCGATGCACCAATTTTATGTGCGGAAGGGGCGATTTTTTCCAGCAATCGCAAGGTATCTTCCGTTAGCGGTCGACGATCTCCAGTGTGCGGATCGGTGATGACGCCTTCAAGCCCATAGCGACAGGCCTGGAAACG       <  SRR19973065.1888946/150‑1 (MQ=60)
                                                                                                                                               GGCCTCTATCGCGCTCGATGCACCAATTTTATGTGCGGAAGGGGCGATTTTTTCCAGCAATCGCAAGGTATCTTCCGTTAGCGGTCGACGATCTCCAGTGTGCGGATCGGTGATGACGCCTTCAAGCCCATAGCGACAGGCCTGGAAACG       <  SRR19973065.640237/150‑1 (MQ=60)
                                                                                                                                               GGCCTCTATCGCGCTCGATGCACCAATTTTATGTGCGGAAGGGGCGATTTTTTCCAGCAATCGCAAGGTATCTTCCGTTAGCGGTCGACGATCTCCAGTGTGCGGATCGGTGATGACGCCTTCAAGCCCATAGCGACAGGCCTGGAAACG       <  SRR19973065.1292960/150‑1 (MQ=60)
                                                                                                                                               GGCCTCTATCGCGCTCGATGCACCAATTTTATGTGCGGAAGGGGCGATTTTTTCCAGCAATCGCAAGGTATCTTCCGTTAGCGGTCGACGATCTCCAGTGTGCGGATCGGTGATGACGCCTTCAAGCCCATAGCGACAGGCCTGGAAACG       <  SRR19973065.3114258/150‑1 (MQ=60)
                                                                                                                                               GGCCTCTATCGCGCTCGATGCACCAATTTTATGTGCGGAAGGGGCGATTTTTTCCAGCAATCGCAAGGTATCTTCCGTTAGCGGTCGACGATCTCCAGTGTGCGGATCGGTGATGACGCCTTCAAGCCCATAGCGACAGGCCTGGAAACG       <  SRR19973065.442888/150‑1 (MQ=60)
                                                                                                                                               GGCCTCTATCGCGCTCGATGCACCAATTTTATGTGCGGAAGGGGCGATTTTTTCCAGCAATCGCAAGGTATCTTCCGTTAGCGGTCGACGATCTCCAGTGTGCGGATCGGTGATGACGCCTTCAAGCCCATAGCGACAGGCCTGGAAACG       <  SRR19973065.3114150/150‑1 (MQ=60)
                                                                                                                                               GGCCTCTATCGCGCTCGATGCACCAATTTTATGTGCGGAAGGGGCGATTTTTTCCAGCAATCGCAAGGTATCTTCCGTTAGCGGTCGACGATCTCCAGTGTGCGGATCGGTGATGACGCCTTCAAGCCCATAGCGACAGGCCTGGAAACG       <  SRR19973065.3114209/150‑1 (MQ=60)
                                                                                                                                                GCCTCAATCGCGCTCGATGCACCAATTTTATGTGCGGAAGGGGCGATTTTTTCCAGCAATCGCAAGGTATCTTCCGTTAGCGGTCGACGATCTCCAGTGTGCGGATCGGTGATGACGCCTTCAAGCCCATAGCGACAGGCCTGGAAACGG      <  SRR19973065.2538700/150‑1 (MQ=60)
                                                                                                                                                    CTATCGCGCTCGATGCACCAATTTTATGTGCGGAAGGGGCGATTTTTTCCAGCAATCGCAAGGTATCTTCCGTTAGCGGTCGACGATCTCCAGTGTGCGGATCGGTGATGACGCCTTCAAGCCCATAGCGACAGGCCTGGAAACGGTTGA  <  SRR19973065.2069868/150‑1 (MQ=60)
                                                                                                                                                    CTATCGCGCTCGATGCACCAATTTTATGTGCGGAAGGGGCGATTTTTTCCAGCAATCGCAAGGTATCTTCCGTTAGCGGTCGACGATCTCCAGTGTGCGGATCGGTGATGACGCCTTCAAGCCCATAGCGACAGGCCTGGAAACGGTTGA  <  SRR19973065.2069942/150‑1 (MQ=60)
                                                                                                                                                     |                                                                                                                                                    
TCTTCTGTAAGGCAATTCCCGGCTTAGTCACCGGCCCAGATCTCACAATGCTTTTTCACCAGCCCAATCAGCGAGCCGCCATCGGCGACGAAATCGCGCATTAGCTGCGCTTCATTCAGACCGCTGACGACCTGGCGATGCAGGGCCTCAATCGCGCTCGATGCACCAATTTTATGTGCGGAAGGGGCGATTTTTTCCAGCAATCGCAAGGTATCTTCCGTTAGCGGTCGACGATCTCCAGTGTGCGGATCGGTGATGACGCCTTCAAGCCCATAGCGACAGGCCTGGAAACGGTTGA  >  CP009273/601698‑601995

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑