Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F434 I0 R1 1130 215.9 7031662 97.1% 6827743 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,735,082 G→T 100% V305V (GTG→GTT)  ydhC → putative arabinose efflux transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,735,0820GT95.0% 656.2 / 22.8 222V305V (GTG→GTT) ydhCputative arabinose efflux transporter
Reads supporting (aligned to +/- strand):  ref base G (7/4);  new base T (102/108);  total (110/112)
Fisher's exact test for biased strand distribution p-value = 3.70e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.70e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 226 total aligned reads displayed.

TGGCTGTCGCGCCGCGCTGCAGAAATGGCAAGGCAAGCAGTTATTACCGTGGTTGCTGGTGCTGTTTGCTGTCAGCGTCATTGCGACCTGGGCTGCGGGCTTCATTAGCCATGTGTCGCTGGTCGAAATCCTGATCCCATTCTGTGTGATGGCGATTGCCAATGGCGCGATCTACCCTATTGTTGTCGCCCAGGCGCTGCGTCCCTTCCCACACGCAACTGGTCGCGCCGCAGCGTTGCAGAACACTCTTCAACTGGGTCTGTGCTTCCTCGCAAGTCTGGTAGTTTCCTGGC  >  CP009273/1734935‑1735227
                                                                                                                                                   |                                                                                                                                                 
  gCTGTCGCGCCGCGCTGCAGAAATGGCAAGGCAAGCAGTTATTACCGTGGTTGCTGGTGCTGTTTGCTGTCAGCGTCATTGCGACCTGGGCTGCGGGCTTCATTAGCCATGTGTCGCTGGTCGAAATCCTGATCCCATTCTGTGTTAt                                                                                                                                                 <  1:3269973/148‑1 (MQ=255)
   cTGTCGCGCCGCGCTGCAGAAATGGCAAGGCAAGCAGTTATTACCGTGGTTGCTGGTGCTGTTTGCTGTCAGCGTCATTGCGACCTGGGCTGCGGGCTTCATTAGCCATGTGTCGCTGGTCGAAATCCTGATCCCATTCTGTGTTATg                                                                                                                                                >  1:1674722/1‑148 (MQ=255)
    tGTCGCGCCGCGCTGCAGAAATGGCAAGGCAAGCAGTTATTACCGTGGTTGCTGGTGCTGTTTGCTGTCAGCGTCATTGCGACCTGGGCTGCGGGCTTCATTAGCCATGTGTCGCTGGTCGAAATCCTGATCCCATTCTGTGTTATgg                                                                                                                                               <  1:1600986/148‑1 (MQ=255)
      tCGCGCCGCGCTGCAGAAATGGCAAGGCAAGCAGTTATTACCGTGGTTGCTGGTGCTGTTTGCTGTCAGCGTCATTGCGACCTGGGCTGCGGGCTTCATTAGCCATGTGTCGCTGGTCGAAATCCTGATCCCATTCTGTGTTATGGCg                                                                                                                                             <  2:2855964/148‑1 (MQ=255)
       cgcgccgcgcTGCAGAAATGGCAAGGCAAGCAGTTATTACCGTGGTTGCTGGTGCTGTTTGCTGTCAGCGTCATTGCGACCTGGGCTGCGGGCTTCATTAGCCATGTGTCGCTGGTCGAAATCCTGATCCCATTCTGTGTTATGGCGa                                                                                                                                            <  2:1240604/148‑1 (MQ=255)
          gccgcgcTGCAGAAATGGCAAGGCAAGCAGTTATTACCGTGGTTGCTGGTGCTGTTTGCTGTCAGCGTCATTGCGACCTGGGCTGCGGGCTTCATTAGCCATGTGTCGCTGGTCGAAATCCTGATCCCATTCTGTGTTATGGCGATTg                                                                                                                                         <  2:146839/148‑1 (MQ=255)
           ccgcgcTGCAGAAATGGCAAGGCAAGCAGTTATTACCGTGGTTGCTGGTGCTGTTTGCTGTCAGCGTCATTGCGACCTGGGCTGCGGGCTTCATTAGCCATGTGTCGCTGGTCGAAATCCTGATCCCATTCTGTGTTATGGCGATTGc                                                                                                                                        <  2:1917080/148‑1 (MQ=255)
           ccgcgcTGCAGAAATGGCAAGGCAAGCAGTTATTACCGTGGTTGCTGGTGCTGTTTGCTGTCAGCGTCATTGCGACCTGGGCTGCGGGCTTCATTAGCCATGTGTCGCTGGTCGAAATCCTGATCCCATTCTGTGTTATGGCGATTGc                                                                                                                                        >  2:3350097/1‑148 (MQ=255)
           ccgcgcTGCAGAAATGGCAAGGCAAGCAGTTATTACCGTGGTTGCTGGTGCTGTTTGCTGTCAGCGTCATTGCGACCTGGGCTGCGGGCTTCATTAGCCATGTGTCGCTGGTCGAAATCCTGATCCCATTCTGTGTTATGGCGATTGc                                                                                                                                        <  2:698696/148‑1 (MQ=255)
           ccgcgcTGCAGAAATGGCAAGGCAAGCAGTTATTACCGTGGTTGCTGGTGCTGTTTGCTGTCAGCGTCATTGCGACCTGGGCTGCGGGCTTCATTAGCCATGTGTCGCTGGTCGAAATCCTGATCCCATTCTGTGTTATGGCGATTGc                                                                                                                                        <  2:698978/148‑1 (MQ=255)
              cgcTGCAGAAATGGCAAGGCAAGCAGTTATTACCGTGGTTGCTGGTGCTGTTTGCTGTCAGCGTCATTGCGACCTGGGCTGCGGGCTTCATTAGCCATGTGTCGCTGGTCGAAATCCTGATCCCATTCTGTGTTATGGCGATTGCCaa                                                                                                                                     <  2:789536/148‑1 (MQ=255)
               gcTGCAGAAATGGCAAGGCAAGCAGTTATTACCGTGGTTGCTGGTGCTGTTTGCTGTCAGCGTCATTGCGACCTGGGCTGCGGGCTTCATTAGCCATGTGTCGCTGGTCGAAATCCTGATCCCATTCTGTGTTATGGCGATTGCCAAt                                                                                                                                    <  1:2084511/148‑1 (MQ=255)
               gcTGCAGAAATGGCAAGGCAAGCAGTTATTACCGTGGTTGCTGGTGCTGTTTGCTGTCAGCGTCATTGCGACCTGGGCTGCGGGCTTCATTAGCCATGTGTCGCTGGTCGAAATCCTGATCCCATTCTGTGTTATGGCGATTGCCAAt                                                                                                                                    <  2:2791924/148‑1 (MQ=255)
                cTGCAGAAATGGCAAGGCAAGCAGTTATTACCGTGGTTGCTGGTGCTGTTTGCTGTCAGCGTCATTGCGACCTGGGCTGCGGGCTTCATTAGCCATGTGTCGCTGGTCGAAATCCTGATCCCATTCTGTGTTATGGCGATTGCCAATg                                                                                                                                   <  1:1502374/148‑1 (MQ=255)
                 tGCAGAAATGGCAAGGCAAGCAGTTATTACCGTGGTTGCTGGTGCTGTTTGCTGTCAGCGTCATTGCGACCTGGGCTGCGGGCTTCATTAGCCATGTGTCGCTGGTCGAAATCCTGATCCCATTCTGTGTTATGGCGATTGCCAATgg                                                                                                                                  <  1:3411756/148‑1 (MQ=255)
                   cAGAAATGGCAAGGCAAGCAGTTATTACCGTGGTTGCTGGTGCTGTTTGCTGTCAGCGTCATTGCGACCTGGGCTGCGGGCTTCATTAGCCATGTGTCGCTGGTCGAAATCCTGATCCCATTCTGTGTTATGGCGATTGCCAATGgcg                                                                                                                                >  2:2096361/1‑148 (MQ=255)
                   cAGAAATGGCAAGGCAAGCAGTTATTACCGTGGTTGCTGGTGCTGTTTGCTGTCAGCGTCATTGCGACCTGGGCTGCGGGCTTCATTAGCCATGTGTCGCTGGTCGAAATCCTGATCCCATTCTGTGTTATGGCGATTGCCAATGgcg                                                                                                                                >  1:2189214/1‑148 (MQ=255)
                      aaaTGGCAAGGCAAGCAGTTATTACCGTGGTTGCTGGTGCTGTTTGCTGTCAGCGTCATTGCGACCTGGGCTGCGGGCTTCATTAGCCATGTGTCGCTGGTCGAAATCCTGATCCCATTCTGTGTTATGGCGATTGCCAATGGCGCGa                                                                                                                             >  2:308921/1‑148 (MQ=255)
                       aaTGGCAAGGCAAGCAGTTATTACCGTGGTTGCTGGTGCTGTTTGCTGTCAGCGTCATTGCGACCTGGGCTGCGGGCTTCATTAGCCATGTGTCGCTGGTCGAAATCCTGATCCCATTCTGTGTTATGGCGATTGCCAATGGCGCGAt                                                                                                                            <  1:2278502/148‑1 (MQ=255)
                         tGGCAAGGCAAGCAGTTATTACCGTGGTTGCTGGTGCTGTTTGCTGTCAGCGTCATTGCGACCTGGGCTGCGGGCTTCATTAGCCATGTGTCGCTGGTCGAAATCCTGATCCCATTCTGTGTTATGGCGATTGCCAATGGCGCGATCt                                                                                                                          >  2:2737843/1‑148 (MQ=255)
                         tGGCAAGGCAAGCAGTTATTACCGTGGTTGCTGGTGCTGTTTGCTGTCAGCGTCATTGCGACCTGGGCTGCGGGCTTCATTAGCCATGTGTCGCTGGTCGAAATCCTGATCCCATTCTGTGTTATGGCGATTGCCAATGGCGCGATCt                                                                                                                          >  2:1774225/1‑148 (MQ=255)
                               ggcaagCAGTTATTACCGTGGTTGCTGGTGCTGTTTGCTGTCAGCGTCATTGTGACCTGGGCTGCGGGCTTCATTAGCCATGTGTCGCTGGTCGAAATCCTGATCCCATTCTGTGTTATGGCGATTGCCAATGGCGCGATCTACCCTa                                                                                                                    <  1:283718/148‑1 (MQ=255)
                               ggcaagCAGTTATTACCGTGGTTGCTGGTGCTGTTTGCTGTCAGCGTCATTGCGACCTGGGCTGCGGGCTTCATTAGCCATGTGTCGCTGGTCGAAATCCTGATCCCATTCTGTGTTATGGCGATTGCCAATGGCGCGATCTACCCTa                                                                                                                    >  2:3392615/1‑148 (MQ=255)
                               ggcaagCAGTTATTACCGTGGTTGCTGGTGCTGTTTGCTGTCAGCGTCATTGCGACCTGGGCTGCGGGCTTCATTAGCCATGTGTCGCTGGTCGAAATCCTGATCCCATTCTGTGTTATGGCGATTGCCAATGGCGCGATCTACCCTa                                                                                                                    >  2:2548208/1‑148 (MQ=255)
                                  aagCAGTTATTACCGTGGTTGCTGGTGCTGTTTGCTGTCAGCGTCATTGCGACCTGGGCTGCGGGCTTCATTAGCCATGTGTCGCTGGTCGAAATCCTGATCCCATTCTGTGTTATGGCGATTGCCAATGGCGCGATCTACCCTAttg                                                                                                                 <  1:1030306/148‑1 (MQ=255)
                                  aagCAGTTATTACCGTGGTTGCTGGTGCTGTTTGCTGTCAGCGTCATTGCGACCTGGGCTGCGGGCTTCATTAGCCATGTGTCGCTGGTCGAAATCCTGATCCCATTCTGTGTTATGGCGATTGCCAATGGCGCGATCTACCCTAttg                                                                                                                 <  2:2640978/148‑1 (MQ=255)
                                    gCAGTTATTACCGTGGTTGCTGGTGCTGTTTGCTGTCAGCGTCATTGCGACCTGGGCTGCGGGCTTCATTAGCCATGTGTCGCTGGTCGAAATCCTGATCCCATTCTGTGTTATGGCGATTGCCAATGGCGCGATCTACCCTAttgtt                                                                                                               >  2:3451811/1‑148 (MQ=255)
                                     cAGTTATTACCGTGGTTGCTGGTGCTGTTTGCTGTCAGCGTCATTGCGACCTGGGCTGCGGGCTTCATTAGCCATGTGTCGCTGGTCGAAATCCTGATCCCATTCTGTGTTATGGCGATTGCCAATGGCGCGATCTACCCTAttgttg                                                                                                              >  1:1842889/1‑148 (MQ=255)
                                     cAGTTATTACCGTGGTTGCTGGTGCTGTTTGCTGTCAGCGTCATTGCGACCTGGGCTGCGGGCTTCATTAGCCATGTGTCGCTGGTCGAAATCCTGATCCCATTCTGTGTTATGGCGATTGCCAATGGCGCGATCTACCCTAttgttg                                                                                                              >  1:2489715/1‑148 (MQ=255)
                                        ttattaCCGTGGTTGCTGGTGCTGTTTGCTGTCAGCGTCATTGCGACCTGGGCTGCGGGCTTCATTAGCCATGTGTCGCTGGTCGAAATCCTGATCCCATTCTGTGTTATGGCGATTGCCAATGGCGCGATCTACCCTATTGTTGTCg                                                                                                           >  1:1236596/1‑148 (MQ=255)
                                          attaCCGTGGTTGCTGGTGCTGTTTGCTGTCAGCGTCATTGCGACCTGGGCTGCGGGCTTCATTAGCCATGTGTCGCTGGTCGAAATCCTGATCCCATTCTGTGTTATGGCGATTGCCAATGGCGCGATCTACCCTATTGTTGTCGcc                                                                                                         >  2:2883582/1‑148 (MQ=255)
                                             aCCGTGGTTGCTGGTGCTGTTTGCTGTCAGCGTCATTGCGACCTGGGCTGCGGGCTTCATTAGCCATGTGTCGCTGGTCGAAATCCTGATCCCATTCTGTGTTATGGCGATTGCCAATGGCGCGATCTACCCTATTGTTGTCGCCCAg                                                                                                      <  2:3044256/148‑1 (MQ=255)
                                                gTGGTTGCTGGTGCTGTTTGCTGTCAGCGTCATTGCGACCTGGGCTGCGGGCTTCATTAGCCATGTGTCGCTGGTCGAAATCCTGATCCCATTCTGTGTTATGGCGATTGCCAATGGCGCGATCTACCCTATTGTTGTCGCCCAGgcg                                                                                                   <  1:60658/148‑1 (MQ=255)
                                                gTGGTTGCTGGTGCTGTTTGCTGTCAGCGTCATTGCGACCTGGGCTGCGGGCTTCATTAGCCATGTGTCGCTGGTCGAAATCCTGATCCCATTCTGTGTTATGGCGATTGCCAATGGCGCGATCTACCCTATTGTTGTCGCCCAGgcg                                                                                                   <  1:2580950/148‑1 (MQ=255)
                                                gTGGTTGCTGGTGCTGTTTGCTGTCAGCGTCATTGCGACCTGGGCTGCGGGCTTCATTAGCCATGTGTCGCTGGTCGAAATCCTGATCCCATTCTGTGTTATGGCGATTGCCAATGGCGCGATCTACCCTATTGTTGTCGCCCAGgcg                                                                                                   >  2:2901596/1‑148 (MQ=255)
                                                 tGGTTGCTGGTGCTGTTTGCTGTCAGCGTCATTGCGACCTGGGCTGCGGGCTTCATTAGCCATGTGTCGCTGGTCGAAATCCTGATCCCATTCTGTGTTATGGCGATTGCCAATGGCGCGATCTACCCTATTGTTGTCGCCCAGgcgc                                                                                                  >  2:2383940/1‑148 (MQ=255)
                                                   gTTGCTGGTGCTGTTTGCTGTCAGCGTCATTGCGACCTGGGCTGCGGGCTTCATTAGCCATGTGTCGCTGGTCGAAATCCTGATCCCATTCTGTGTTATGGCGATTGCCAATGGCGCGATCTACCCTATTGTTGTCGCCCAGGCGCTg                                                                                                <  1:1848087/148‑1 (MQ=255)
                                                         ggTGCTGTTTGCTGTCAGCGTCATTGCGACCTGGGCTGCGGGCTTCATTAGCCATGTGTCGCTGGTCGAAATCCTGATCCCATTCTGTGTTATGGCGATTGCCAATGGCGCGATCTACCCTATTGTTGTCGCCCAGGCGCTGCGTccc                                                                                          <  2:3263985/148‑1 (MQ=255)
                                                              tGTTTGCTGTCAGCGTCATTGCGACCTGGGCTGCGGGCTTCATTAGCCATGTGTCGCTGGTCGAAATCCTGATCCCATTCTGTGTTATGGCGATTGCCAATGGCGCGATCTACCCTATTGTTGTCGCCCAGGCGCTGCGTCCCTTccc                                                                                     <  2:202294/148‑1 (MQ=255)
                                                              tGTTTGCTGTCAGCGTCATTGCGACCTGGGCTGCGGGCTTCATTAGCCATGTGTCGCTGGTCGAAATCCTGATCCCATTCTGTGTTATGGCGATTGCCAATGGCGCGATCTACCCTATTGTTGTCGCCCAGGCGCTGCGTCCCTTccc                                                                                     <  2:201939/148‑1 (MQ=255)
                                                                       tCAGCGTCATTGCGACCTGGGCTGCGGGCTTCATTAGCCATGTGTCGCTGGTCGAAATCCTGATCCCATTCTGTGTTATGGCGATTGCCAATGGCGCGATCTACCCTATTGTTGTCGCCCAGGCGCTGCGTCCCTTCCCACACGCAAc                                                                            >  1:221930/1‑148 (MQ=255)
                                                                         aGCGTCATAGCGACCTGGGCTGCGGGAATCATTAGCCAACATTCGCTGGTCGAAATCCTGATCCCATTCTGTGTAATGGCCATTGCCAATGGCGCGATCTACACTATTGTTGTCGCCCAGGCGCTGCTTCCCTTACCACACGCAACTg                                                                          >  2:3502888/1‑148 (MQ=255)
                                                                          gCGTCATTGCGACCTGGGCTGCGGGCTTCATTAGCCATGTGTCGCTGGTCGAAATCCTGATCCCATTCTGTGTTATGGCGATTGCCAATGGCGCGATCTACCCTATTGTTGTCGCCCAGGCGCTGCGTCCCTTCCCACACGCAACTgg                                                                         <  1:3204342/148‑1 (MQ=255)
                                                                          gCGTCATTGCGACCTGGGCTGCGGGCTTCATTAGCCATGTGTCGCTGGTCGAAATCCTGATCCCATTCTGTGTTATGGCGATTGCCAATGGCGCGATCTACCCTATTGTTGTCGCCCAGGCGCTGCGTCCCTTCCCACACGCAACTgg                                                                         <  2:2104995/148‑1 (MQ=255)
                                                                             tCATTGCGACCTGGGCTGCGGGCTTCATTAGCCATGTGTCGCTGGTCGAAATCCTGATCCCATTCTGTGTTATGGCGATTGCCAATGGCGCGATCTACCCTATTGTTGTCGCCCAGGCGCTGCGTCCCTTCCCACACGCAACTGGTcg                                                                      >  1:2558576/1‑148 (MQ=255)
                                                                             tCATTGCGACCTGGGCTGCGGGCTTCATTAGCCATGTGTCGCTGGTCGAAATCCTGATCCCATTCTGTGTTATGGCGATTGCCAATGGCGCGATCTACCCTATTGTTGTCGCCCAGGCGCTGCGTCCCTTCCCACACGCAACTGGTcg                                                                      >  1:945598/1‑148 (MQ=255)
                                                                                     aCCTGGGCTGCGGGCTTCATTAGCCATGTGTCGCTGGTCGAAATCCTGATCCCATTCTGTGTTATGGCGATTGCCAATGGCGCGATCTACCCTATTGTTGTCGCCCAGGCGCTGCGTCCCTTCCCACACGCAACTGGTCGCGCCGCAg                                                              <  1:613663/148‑1 (MQ=255)
                                                                                      ccTGGGCTGCGGGCTTCATTAGCCATGTGTCTCTGGTCGAAATCCTGATCCCATTCTGTGTTATGGCGATTGCCAATGGCGCGATCTACCCTATTGTTGTCGCCCAGGCGCTGCGTCCCTTCCCACACGCAACTGGTCGCGCCGCAGc                                                             >  1:1935847/1‑148 (MQ=255)
                                                                                      ccTGGGCTGCGGGCTTCATTAGCCATGTGTCTCTGGTCGAAATCCTGATCCCATTCTGTGTTATGGCGATTGCCAATGGCGCGATCTACCCTATTGTTGTCGCCCAGGCGCTGCGTCCCTTCCCACACGCAACTGGTCGCGCCGCAGc                                                             >  1:2334256/1‑148 (MQ=255)
                                                                                      ccTGGGCTGCGGGCTTCATTAGCCATGTGTCGCTGGTCGAAATCCTGATCCCATTCTGTGTTATGGCGATTGCCAATGGCGCGATCTACCCTATTGTTGTCGCCCAGGCGCTGCGTCCCTTCCCACACGCAACTGGTCGCGCCGCAGc                                                             >  2:2553695/1‑148 (MQ=255)
                                                                                       cTGGGCTGCGGGCTTCATTAGCCATGTGTCGCTGGTCGAAATCCTGATCCCATTCTGTGTTATGGCGATTGCCAATGGCGCGATCTACCCTATTGTTGTCGCCCAGGCGCTGCGTCCCTTCCCACACGCAACTGGTCGCGCCGCAGCg                                                            <  2:1888028/148‑1 (MQ=255)
                                                                                        tGGGCTGCGGGCTTCATTAGCCATGTGTCGCTGGTCGAAATCCTGATCCCATTCTGTGTTATGGCGATTGCCAATGGCGCGATCTACCCTATTGTTGTCGCCCAGGCGCTGCGTCCCTTCCCACACGCAACTGGTCGCGCCGCAGCGt                                                           <  1:284826/148‑1 (MQ=255)
                                                                                        tGGGCTGCGGGCTTCATTAGCCATGTGTCGCTGGTCGAAATCCTGATCCCATTCTGTGTGATGGCGATTGCCAATGGCGCGATCTACCCTATTGTTGTCGCCCAGGCGCTGCGTCCCTTCCCACACGCAACTGGTCGCGCCGCAGCGt                                                           >  2:811053/1‑148 (MQ=255)
                                                                                          ggCTGCGGGCTTCATTAGCCATGTGTCGCTGGTCGAAATCCTGATCCCATTCTGTGTTATGGCGATTGCCAATGGCGCGATCTACCCTATTGTTGTCGCCCAGGCGCTGCGTCCCTTCCCACACGCAACTGGTCGCGCCGCAGCGTTg                                                         <  2:1789303/148‑1 (MQ=255)
                                                                                          ggCTGCGGGCTTCATTAGCCATGTGTCGCTGGTCGAAATCCTGATCCCATTCTGTGTTATGGCGATTGCCAATGGCGCGATCTACCCTATTGTTGTCGCCCAGGCGCTGCGTCCCTTCCCACACGCAACTGGTCGCGCCGCAGCGTTg                                                         >  1:803213/1‑148 (MQ=255)
                                                                                                gggCTTCATTAGCCATGTGTCGCTGGTCGAAATCCTGCTCCCATTCTGTGTTATGGCGATTGCCAATGGCGCGATCTACCCTATTGTTGTCGCCCAGGCGCTGCGTCCCTTCCCACACGCAACTGGTCGCGCCGCAGCGTTGCAGAac                                                   <  2:1711845/148‑1 (MQ=255)
                                                                                                 ggCTTCATTAGCCATGTGTCGCTTGTCGAAATCCTGATCCCATTCTGTGTTATGGCGATTGCCAATGGCGCGATCTACCCTATTGTTGTCGCCCAGGCGCTGCGTCCCTTCCCTCACGCAACTGGTCGCGCCGCAGCGTTGCAGAaca                                                  <  2:3265221/148‑1 (MQ=255)
                                                                                                 ggCTTCATTAGCCATGTGTCGCTGGTCGAAATCCTGATCCCATTCTGTGTTATGGCGATTGCCAATGGCGCGATCTACCCTATTGTTGTCGCCCAGGCGCTGCGTCCCTTCCCACACGCAACTGGTCGCGCCGCAGCGTTGCAGAaca                                                  <  2:3098572/148‑1 (MQ=255)
                                                                                                  gCTTCATTAGCCATGTGTCGCTGGTCGAAATCCTGATCCCATTCTGTGTTATGGCGATTGCCAATGGCGCGATCTACCCTATTGTTGTCGCCCAGGCGCTGCGTCCCTTCCCACACGCAACTGGTCGCGCCGCAGCGTTGCAGAacac                                                 >  2:212392/1‑148 (MQ=255)
                                                                                                    ttCATTAGCCATGTGTCGCTGGTCGAAATCCTGATCCCATTCTGTGTTATGGCGATTGCCAATGGCGCGATCTACCCTATTGTTGTCGCCCAGGCGCTGCGTCCCTTCCCACACGCAACTGGTCGCGCCGCAGCGTTGCAGAACActc                                               >  2:3134281/1‑148 (MQ=255)
                                                                                                    ttCATTAGCCATGTGTCGCTGGTCGAAATCCTGATCCCATTCTGTGTTATGGCGATTGCCAATGGCGCGATCTACCCTATTGTTGTCGCCCAGGCGCTGCGTCCCTTCCCACACGCAACTGGTCGCGCCGCAGCGTTGCAGAACActc                                               <  1:749753/148‑1 (MQ=255)
                                                                                                     tCATTAGCCATGTGTCGCTGGTCGAAATCCTGATCCCATTCTGTGTTATGGCGATTGCCAATGGCGCGATCTACCCTATTGTTGTCGCCCAGGCGCTGCGTCCCTTCCCACACGCAACTGGTCGCGCCGCAGCGTTGCAGAACActct                                              >  2:1345024/1‑148 (MQ=255)
                                                                                                       aTTAGCCATGTGTCGCTGGTCGAAATCCTGATCCCATTCTGTGTTATGGCGATTGCCAATGGCGCGATCTACCCTATTGTTGTCGCCCAGGCGCTGCGTCCCTTCCCACACGCAACTGGTCGCGCCGCAGCGTTGCAGAACACTCTTc                                            <  2:1107302/148‑1 (MQ=255)
                                                                                                           gCCATGTGTCGCTGGTCGAAATCCTGATCCCATTCTGTGTTATGGCGATTGCCAATGGCGCGATCTACCCTATTGTTGTCGCCCAGGCGCTGCGTCCCTTCCCACACGCAACTGGTCGCGCCGCAGCGTTGCAGAACACTCTTCAACt                                        <  1:2855354/148‑1 (MQ=255)
                                                                                                            ccATGTGTCGCTGGTCGAAATCCTGATCCCATTCTGTGTTATGGCGATTGCCAATGGCGCGATCTACCCTATTGTTGTCGCCCAGGCGCTGCGTCCCTTCCCACACGCAACTGGTCGCGCCGCAGCGTTGCAGAACACTCTTCAACTg                                       >  1:2407383/1‑148 (MQ=255)
                                                                                                              aTGTGTCGCTGGTCGAAATCCTGATCCCATTCTGTGTTATGGCGATTGCCAATGGCGCGATCTACCCTATTGTTGTCGCCCAGGCGCTGCGTCCCTTCCCACACGCAACTGGTCGCGCCGCAGCGTTGCAGAACACTCTTCAACTggg                                     <  1:725003/148‑1 (MQ=255)
                                                                                                                gtgtCGCTGGTCGAAATCCTGATCCCATTCTGTGTTATGGCGATTGCCAATGGCGCGATCTACCCTATTGTTGTCGCCCAGGCGCTGCGTCCCTTCCCACACGCAACTGGTCGCGCCGCAGCGTTGCAGAACACTCTTCAACTGGGTc                                   >  2:1040575/1‑148 (MQ=255)
                                                                                                                 tgtCGCTGGTCGAAATCCTGATCCCATTCTGTGTTATGGCGATTGCCAATGGCGCGATCTACCCTATTGTTGTCGCCCAGGCGCTGCGTCCCTTCCCACACGCAACTGGTCGCGCCGCAGCGTTGCAGAACACTCTTCAACTGGGTCt                                  >  1:3073207/1‑148 (MQ=255)
                                                                                                                    cGCTGGTCGAAATCCTGATCCCATTCTGTGTTATGGCGATTGCCAATGGCGCGATCTACCCTATTGTTGTCGCCCAGGCGCTGCGTCCCTTCCCACACGCAACTGGTCGCGCCGCAGCGTTGCAGAACACTCTTCAACTGGGTCtgtg                               <  2:2157155/148‑1 (MQ=255)
                                                                                                                    cGCTGGTCGAAATCCTGATCCCATTCTGTGTTATGGCGATTGCCAATGGCGCGATCTACCCTATTGTTGTCGCCCAGGCGCTGCGTCCCTTCCCACACGCAACTGGTCGCGCCGCAGCGTTGCAGAACACTCTTCAACTGGGTCtgtg                               <  1:2028723/148‑1 (MQ=255)
                                                                                                                     gCTGGTCGAAATCCTGATCCCATTCTGTGTTATGGCGATTGCCAATGGCGCGATCTACCCTATTGTTGTCGCCCAGGCGCTGCGTCCCTTCCCACACGCAACTGGTCGCGCCGCAGCGTTGCAGAACACTCTTCAACTGGGTCTGTGc                              <  2:1842933/148‑1 (MQ=255)
                                                                                                                     gCTGGTCGAAATCCTGATCCCATTCTGTGTTATGGCGATTGCCAATGGCGCGATCTACCCTATTGTTGTCGCCCAGGCGCTGCGTCCCTTCCCACACGCAACTGGTCGCGCCGCAGCGTTGCAGAACACTCTTCAACTGGGTCTGTGc                              <  2:804425/148‑1 (MQ=255)
                                                                                                                         gTCGAAATCCTGATCCCATTCTGTGTTATGGCGATTGCCAATGGCGCGATCTACCCTATTGTTGTCGCCCAGGCGCTGCGTCCCTTCCCACACGCAACTGGTCGCGCCGCAGCGTTGCAGAACACTCTTCAACTGGGTCTGTGCTTcc                          >  1:1001295/1‑148 (MQ=255)
                                                                                                                         gTCGAAATCCTGATCCCATTCTGTGTTATGGCGATTGCCAATGGCGCGATCTACCCTATTGTTGTCGCCCAGGCGCTGCGTCCCTTCCCACACGCAACTGGTCGCGCCGCAGCGTTGCAGAACACTCTTCAACTGGGTCTGTGCTTcc                          >  2:2824605/1‑148 (MQ=255)
                                                                                                                           cGAAATCCTGATCCCATTCTGTGTTATGGCGATTGCCAATGGCGCGATCTACCCTATTGTTGTCGCCCAGGCGCTGCGTCCCTTCCCACACGCAACTGGTCGCGCCGCAGCGTTGCAGAACACTCTTCAAc                                         <  1:247631/131‑1 (MQ=255)
                                                                                                                           cGAAATCCTGATCCCATTCTGTGTTATGGCGATTGCCAATGGCGCGATCTACCCTATTGTTGTCGCCCAGGCGCTGCGTCCCTTCCCACACGCAACTGGTCGCGCCGCAGCGTTGCAGAACACTCTTCAAc                                         >  2:247631/1‑131 (MQ=255)
                                                                                                                              aaTCCTGATCCCATTCTGTGTTATGGCGATTGCCAATGGCGCGATCTACCCTATTGTTGTCGCCCAGGCGCTGCGTCCCTTCCCACACGCAACTGGTCGCGCCGCAGCGTTGCAGAACACTCTTCAACTGGGTCTGTGCTTCCTCGCa                     >  1:62432/1‑148 (MQ=255)
                                                                                                                              aaTCCTGATCCCATTCTGTGTGATGGCGATTGCCAATGGCGCGATCTACCCTATTGTTGTCGCCCAGGCGCTGCGTCCCTTCCCACACGCAACTGGTCGCGCCGCAGCGTTGCAGAACACTCTTCAACTGGGTCTGTGCTTCCTCGCa                     <  2:423930/148‑1 (MQ=255)
                                                                                                                               aTCCTGATCCCATTCTGTGTTATGGCGATTGCCAATGGCGCGATCTACCCTATTGTTGTCGCCCAGGCGCTGCGTCCCTTCCCACACGCAACTGGTCGCGCCGCAGCGTTGCAGAACACTCTTCAACTGGGTCTGTGCTTCCTCGCaa                    >  1:2410601/1‑148 (MQ=255)
                                                                                                                                tCCTGATCCCATTCTGTGTTATGGCGATTGCCAATGGCGCGATCTACCCTATTGTTGTCGCCCAGGCGCTGCGTCCCTTCCCACACGCAACTGGTCGCGCCGCAGCGTTGCAGAACACTCTTCAACTGGGTCTGTGCTTCCTCGCAAg                   >  1:3304442/1‑148 (MQ=255)
                                                                                                                                tCCTGATCCCATTCTGTGTTATGGCGATTGCCAATGGCGCGATCTACCCTATTGTTGTCGCCCAGGCGCTGCGTCCCTTCCCACACGCAACTGGTCGCGCCGCAGCGTTGCAGAACACTCTTCAACTGGGTCTGTGCTTCCTCGCAAg                   >  1:659409/1‑148 (MQ=255)
                                                                                                                                 ccTGATCCCATTCTGTGTTATGGCGATTGCCAATGGCGCGATCTACCCTATTGTTGTCGCCCAGGCGCTGCGTCCCTTCCCACACGCAACTGGTCGCGCCGCAGCGTTGCAGAACACTCTTCAACTGGGTCTGTGCTTCCTCGCAAGt                  >  2:1807881/1‑148 (MQ=255)
                                                                                                                                  cTGATCCCATTCTGTGTTATGGCGATTGCCAATGGCGCGATCTACCCTATTGTTGTCGCCCAGGCGCTGCGTCCCTTCCCACACGCAACTGGTCGCGCCGCAGCGTTGCAGAACACTCTTCAACTGGGTCTGTGCTTCCTCGCAAGTc                 <  2:2489715/148‑1 (MQ=255)
                                                                                                                                  cTGATCCCATTCTGTGTTATGGCGATTGCCAATGGCGCGATCTACCCTATTGTTGTCGCCCAGGCGCTGCGTCCCTTCCCACACGCAACTGGTCGCGCCGCAGCGTTGCAGAACACTCTTCAACTGGGTCTGTGCTTCCTCGCAAGTc                 <  2:727238/148‑1 (MQ=255)
                                                                                                                                   tGATCCCATTCTGTGTTATGGCGATTGCCAATGGCGCGATCTACCCTATTGTTGTCGCCCAGGCGCTGCGTCCCTTCCCACACGCAACTGGTCGCGCCGCAGCGTTGCAGAACACTCTTCAACTGGGTCTGTGCTTCCTCGCAAGTCt                >  2:2946210/1‑148 (MQ=255)
                                                                                                                                   tGATCCCATTCTGTGTTATGGCGATTGCCAATGGCGCGATCTACCCTATTGTTGTCGCCCAGGCGCTGCGTCCCTTCCCACACGCAACTGGTCGCGCCGCAGCGTTGCAGAACACTCTTCAACTGGGTCTGTGCTTCCTCGCAAGTCt                >  1:1669329/1‑148 (MQ=255)
                                                                                                                                       cccATTCTGTGTTATGGCGATTGCCAATGGCGCGATCTACCCTATTGTTGTCGCCCAGGCGCTGCGTCCCTTCCCACACGCAACTGGTCGCGCCGCAGCGTTGCAGAACACTCTTCAACTGGGTCTGTGCTTCCTCGCAAGTCTGGTa            >  1:2892178/1‑148 (MQ=255)
                                                                                                                                       cccATTCTGTGTTATGGCGATTGCCAATGGCGCGATCTACCCTATTGTTGTCGCCCAGGCGCTGCGTCCCTTCCCACACGCAACTGGTCGCGCCGCAGCGTTGCAGAACACTCTTCAACTGGGTCTGTGCTTCCTCGCAAGTCTGGTa            <  2:2334256/148‑1 (MQ=255)
                                                                                                                                       cccATTCTGTGTTATGGCGATTGCCAATGGCGCGATCTACCCTATTGTTGTCGCCCAGGCGCTGCGTCCCTTCCCACACGCAACTGGTCGCGCCGCAGCGTTGCAGAACACTCTTCAACTGGGTCTGTGCTTCCTCGCAAGTCTGGTa            >  1:663121/1‑148 (MQ=255)
                                                                                                                                       cccATTCTGTGTTATGGCGATTGCCAATGGCGCGATCTACCCTATTGTTGTCGCCCAGGCGCTGCGTCCCTTCCCACACGCAACTGGTCGCGCCGCAGCGTTGCAGAACACTCTTCAACTGGGTCTGTGCTTCCTCGCAAGTCTGGTa            <  2:1935847/148‑1 (MQ=255)
                                                                                                                                         cATTCTGTGTTATGGCGATTGCCAATGGCGCGATCTACCCTATTGTTGTCGCCCAGGCGCTGCGTCCCTTCCCACACGCAACTGGTCGCGCCGCAGCGTTGCAGAACACTCTTCAACTGGGTCTGTGCTTCCTCGCAAGTCTGGTAGt          >  1:2832337/1‑148 (MQ=255)
                                                                                                                                         cATTCTGTGTTATGGCGATTGCCAATGGCGCGATCTACCCTATTGTTGTCGCCCAGGCGCTGCGTCCCTTCCCACACGCAACTGGTCGCGCCGCAGCGTTGCAGAACACTCTTCAACTGGGTCTGTGCTTCCTCGCAAGTCTGGTAGt          >  1:1037757/1‑148 (MQ=255)
                                                                                                                                          aTTCTGTGTTATGGCGATTGCCAATGGCGCGATCTACCCTATTGTTGTCGCCCAGGCGCTGCGTCCCTTCCCACACGCAACTGGTCGCGCCGCAGCGTTGCAGAACACTCTTCAACTGGGTCTGTGCTTCCTCGCAAGTCTGGTAGtt         >  2:2899808/1‑148 (MQ=255)
                                                                                                                                           ttCTGTGTTATGGCGATTGCCAATGGCGCGATCTACCCTATTGTTGTCGCCCAGGCGCTGCGTCCCTTCCCACACGCAACTGGTCGCGCCGCAGCGTTGCAGAACACTCTTCAACTGGGTCTGTGCTTCCTCGCAAGTCTGGTAGttt        >  2:2274414/1‑148 (MQ=255)
                                                                                                                                            tCTGTGTTATGGCGATTGCCAATGGCGCGATCTACCCTATTGTTGTCGCCCAGGCGCTGCGTCCCTTCCCACACGCAACTGGTCGCGCCGCAGCGTTGCAGAACACTCTTCAACTGGGTCTGTGCTTCCTCGCAAGTCTGGTAGTTTc       >  1:3248927/1‑148 (MQ=255)
                                                                                                                                            tCTGTGTTATGGCGATTGCCAATGGCGCGATCTACCCTATTGTTGTCGCCCAGGCGCTGCGTCCCTTCCCACACGCAACTGGTCGCGCCGCAGCGTTGCAGAACACTCTTCAACTGGGTCTGTGCTTCCTCGCAAGTCTGGTAGTTTc       <  1:2291103/148‑1 (MQ=255)
                                                                                                                                             cTGTGTTATGGCGATTGCCAATGGCGCGATCTACCCTATTGTTGTCGCCCAGGCGCTGCGTCCCTTCCCACACGCAACTGGTCGCGCCGCAGCGTTGCAGAACACTCTTCAACTGGGTCTGTGCTTCCTCGCAAGTCTGGTAGTTTcc      <  2:743255/148‑1 (MQ=255)
                                                                                                                                             cTGTGTGATGGCGATTGCCAATGGCGCGATCTACCCTATTGTTGTCGCCCAGGCGCTGCGTCCCTTCCCACACGCAACTGGTCGCGCCGCAGCGTTGCAGAACACTCTTCAACTGGGTCTGTGCTTCCTCGCAAGTCTGGTAGTTTcc      <  2:2412834/148‑1 (MQ=255)
                                                                                                                                                tgttATGGCGATTGCCAATGGCGCGATCTACCCTATTGTTGTCGCCCAGGCGCTGCGTCCCTTCCCACACGCAACTGGTCGCGCCGCAGCGTTGCAGAACACTCTTCAACTGGGTCTGTGCTTCCTCGCAAGTCTGGTAGTTTCCTgg   <  2:1871218/148‑1 (MQ=255)
                                                                                                                                                 gtgATGGCGATTGCCAATGGCGCGATCTACCCTATTGTTGTCGCCCAGGCGCTGCGTCCCTTCCCACACGCAACTGGTCGCGCCGCAGCGTTGCAGAACACTCTTCAACTGGGTCTGTGCTTCCTCGCAAGTCTGGTAGTTTCCTGGc  <  1:3038681/148‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                 
TGGCTGTCGCGCCGCGCTGCAGAAATGGCAAGGCAAGCAGTTATTACCGTGGTTGCTGGTGCTGTTTGCTGTCAGCGTCATTGCGACCTGGGCTGCGGGCTTCATTAGCCATGTGTCGCTGGTCGAAATCCTGATCCCATTCTGTGTGATGGCGATTGCCAATGGCGCGATCTACCCTATTGTTGTCGCCCAGGCGCTGCGTCCCTTCCCACACGCAACTGGTCGCGCCGCAGCGTTGCAGAACACTCTTCAACTGGGTCTGTGCTTCCTCGCAAGTCTGGTAGTTTCCTGGC  >  CP009273/1734935‑1735227

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TATGGCTGTCGCGCCGCGCTGCAGAAATGGCAAGGCAAGCAGTTATTACCGTGGTTGCTGGTGCTGTTTGCTGTCAGCGTCATTGCGACCTGGGCTGCGGGCTTCATTAGCCATGTGTCGCTGGTCGAAATCCTGATCCCATTCTGTGTGATGGCGATTGCCAATGGCGCGATCTACCCTATTGTTGTCGCCCAGGCGCTGCGTCCCTTCCCACACGCAACTGGTCGCGCCGCAGCGTTGCAGAACACTCTTCAACTGGGTCTGTGCTTCCTCGCAAGTCTGGTAGTTTCCTGGCTG  >  CP009273/1734933‑1735229
                                                                                                                                                     |                                                                                                                                                   
TATGGCTGTCGCGCCGCGCTGCAGAAATGGCAAGGCAAGCAGTTATTACCGTGGTTGCTGGTTCTGTTTGCTGTCAGCGTCATTGCGACCTGGGCTGCGGGATTCATTAGCCATGTGTCGCTGGTCGAAATCCTGATCCCATTCTGTGTT                                                                                                                                                     <  SRR19973065.2978571/150‑1 (MQ=60)
   GGCTGTCGCGCCGCGCTGCAGAAATGGCAAGGCAAGCAGTTATTACCGTGGTTGCTGGTGCTGTTTGCTGTCAGCGTCATTGCGACCTGGGCTGCGGGCTTCATTAGCCATGTGTCGCTGGTCGAAATCCTGATCCCATTCTGTGTTATG                                                                                                                                                  >  SRR19973065.1685927/1‑150 (MQ=60)
    GCTGTCGCGCCGCGCTGCAGAAATGGCAAGGCAAGCAGTTATTACCGTGGTTGCTGGTGCTGTTTGCTGTCAGCGTCATTGCGACCTGGGCTGCGGGCTTCATTAGCCATGTGTCGCTGGTCGAAATCCTGATCCCATTCTGTGTTATGG                                                                                                                                                 <  SRR19973065.3289808/150‑1 (MQ=60)
     CTGTCGCGCCGCGCTGCAGAAATGGCAAGGCAAGCAGTTATTACCGTGGTTGCTGGTGCTGTTTGCTGTCAGCGTCATTGCGACCTGGGCTGCGGGCTTCATTAGCCATGTGTCGCTGGTCGAAATCCTGATCCCATTCTGTGTTATGGC                                                                                                                                                <  SRR19973065.2062059/150‑1 (MQ=60)
      TGTCGCGCCGCGCTGCAGAAATGGCAAGGCAAGCAGTTATTACCGTGGTTGCTGGTGCTGTTTGCTGTCAGCGTCATTGCGACCTGGGCTGCGGGCTTCATTAGCCATGTGTCGCTGGTCGAAATCCTGATCCCATTCTGTGTTATGGCG                                                                                                                                               <  SRR19973065.1611686/150‑1 (MQ=60)
                 GCTGCAGAAATGGCAAGGCAAGCAGTTATTACCGTGGTTGCTGGTGCTGTTTGCTGTCAGCGTCATTGCGACCTGGGCTGCGGGCTTCATTAGCCATGTGTCGCTGGTCGAAATCCTGATCCCATTCTGTGTTATGGCGATTGCCAATGG                                                                                                                                    <  SRR19973065.2097874/150‑1 (MQ=60)
                  CTGCAGAAATGGCAAGGCAAGCAGTTATTACCGTGGTTGCTGGTGCTGTTTGCTGTCAGCGTCATTGCGACCTGGGCTGCGGGCTTCATTAGCCATGTGTCGCTGGTCGAAATCCTGATCCCATTCTGTGTTATGGCGATTGCCAATGGC                                                                                                                                   >  SRR19973065.2302157/1‑150 (MQ=60)
                  CTGCAGAAATGGCAAGGCAAGCAGTTATTACCGTGGTTGCTGGTGCTGTTTGCTGTCAGCGTCATTGCGACCTGGGCTGCGGGCTTCATTAGCCATGTGTCGCTGGTCGAAATCCTGATCCCATTCTGTGTTATGGCGATTGCCAATGGC                                                                                                                                   <  SRR19973065.1884184/150‑1 (MQ=60)
                  CTGCAGAAATGGCAAGGCAAGCAGTTATTACCGTGGTTGCTGGTGCTGTTTGCTGTCAGCGTCATTGCGACCTGGGCTGCGGGCTTCATTAGCCATGTGTCGCTGGTCGAAATCCTGATCCCATTCTGTGTTATGGCGATTGCCAATGGC                                                                                                                                   <  SRR19973065.1512088/150‑1 (MQ=60)
                   TGCAGAAATGGCAAGGCAAGCAGTTATTACCGTGGTTGCTGGTGCTGTTTGCTGTCAGCGTCATTGCGACCTGGGCTGCGGGCTTCATTAGCCATGTGTCGCTGGTCGAAATCCTGATCCCATTCTGTGTTATGGCGATTGCCAATGGCG                                                                                                                                  <  SRR19973065.3432601/150‑1 (MQ=60)
                   TGCAGAAATGGCAAGGCAAGCAGTTATTACCGTGGTTGCTGGTGCTGTTTGCTGTCAGCGTCATTGCGACCTGGGCTGCGGGCTTCATTAGCCATGTGTCGCTGGTCGAAATCCTGATCCCATTCTGTGTTATGGCGATTGCCAATGGCG                                                                                                                                  >  SRR19973065.2203254/1‑150 (MQ=60)
                       GAAATGGCAAGGCAAGCAGTTATTACCGTGGTTGCTGGTGCTGTTTGCTGTCAGCGTCATTGCGACCTGGGCTGCGGGCTTCATTAGCCATGTGTCGCTGGTCGAAATCCTGATCCCATTCTGTGTTATGGCGATTGCCAATGGCGCGAT                                                                                                                              >  SRR19973065.638008/1‑150 (MQ=60)
                         AATGGCAAGGCAAGCAGTTATTACCGTGGTTGCTGGTGCTGTTTGCTGTCAGCGTCATTGCGACCTGGGCTGCGGGCTTCATTAGCCATGTGTCGCTGGTCGAAATCCTGATCCCATTCTGTGTTATGGCGATTGCCAATGGCGCGATCT                                                                                                                            <  SRR19973065.2292875/150‑1 (MQ=60)
                             GCAAGGCAAGCAGTTATTACCGTGGTTGCTGGTGCTGTTTGCTGTCAGCGTCATTGCGACCTGGGCTGCGGGCTTCATTAGCCATGTGTCGCTGGTCGAAATCCTGATCCCATTCTGTGTTATGGCGATTGCCAATGGCGCGATCTACCC                                                                                                                        <  SRR19973065.727075/150‑1 (MQ=60)
                                 GGCAAGCAGTTATTACCGTGGTTGCTGGTGCTGTTTGCTGTCAGCGTCATTGTGACCTGGGCTGCGGGCTTCATTAGCCATGTGTCGCTGGTCGAAATCCTGATCCCATTCTGTGTTATGGCGATTGCCAATGGCGCGATCTACCCTATT                                                                                                                    <  SRR19973065.285591/150‑1 (MQ=60)
                                 GGCAAGCAGTTATTACCGTGGTTGCTGGTGCTGTTTGCTGTCAGCGTCATTGTGACCTGGGCTGCGGGCTTCATTAGCCATGTGTCGCTGGTCGAAATCCTGATCCCATTCTGTGTTATGGCGATTGCCAATGGCGCGATCTACCCTATT                                                                                                                    <  SRR19973065.285980/150‑1 (MQ=60)
                                  GCAAGCAGTTATTACCGTGGTTGCTGGTGCTGTTTGCTGTCAGCGTCATTGCGACCTGGGCTGCGGGCTTCATTAGCCATGTGTCGCTGGTCGAAATCCTGATCCCATTCTGTGTTATGGCGATTGCCAATGGCGCGATCTACCCTATTG                                                                                                                   <  SRR19973065.1041307/150‑1 (MQ=60)
                                    AAGCAGTTATTACCGTGGTTGCTGGTGCTGTTTGCTGTCAGCGTCATTGCGACCTGGGCTGCGGGCTTCATTAGCCATGTGTCGCTGGTCGAAATCCTGATCCCATTCTGTGTTATGGCGATTGCCAATGGCGCGATCTACCCTATTGTT                                                                                                                 <  SRR19973065.1037130/150‑1 (MQ=60)
                                    AAGCAGTTATTACCGTGGTTGCTGGTGCTGTTTGCTGTCAGCGTCATTGCGACCTGGGCTGCGGGCTTCATTAGCCATGTGTCGCTGGTCGAAATCCTGATCCCATTCTGTGTTATGGCGATTGCCAATGGCGCGATCTACCCTATTGTT                                                                                                                 >  SRR19973065.2196202/1‑150 (MQ=60)
                                     AGCAGTTATTACCGTGGTTGCTGGTGCTGTTTGCTGTCAGCGTCATTGCGACCTGGGCTGCGGGCTTCATTAGCCATGTGTCGCTGGTCGAAATCCTGATCCCATTCTGTGTTATGGCGATTGCCAATGGCGCGATCTACCCTATTGTTG                                                                                                                >  SRR19973065.2505380/1‑150 (MQ=60)
                                     AGCAGTTATTACCGTGGTTGCTGGTGCTGTTTGCTGTCAGCGTCATTGCGACCTGGGCTGCGGGCTTCATTAGCCATGTGTCGCTGGTCGAAATCCTGATCCCATTCTGTGTTATGGCGATTGCCAATGGCGCGATCTACCCTATTGTTG                                                                                                                >  SRR19973065.1855039/1‑150 (MQ=60)
                                     AGCAGTTATTACCGTGGTTGCTGGTGCTGTTTGCTGTCAGCGTCATTGCGACCTGGGCTGCGGGCTTCATTAGCCATGTGTCGCTGGTCGAAATCCTGATCCCATTCTGTGTTATGGCGATTGCCAATGGCGCGATCTACCCTATTGTTG                                                                                                                >  SRR19973065.1855045/1‑150 (MQ=60)
                                     AGCAGTTATTACCGTGGTTGCTGGTGCTGTTTGCTGTCAGCGTCATTGCGACCTGGGCTGCGGGCTTCATTAGCCATGTGTCGCTGGTCGAAATCCTGATCCCATTCTGTGTTATGGCGATTGCCAATGGCGCGATCTACCCTATTGTTG                                                                                                                >  SRR19973065.1855089/1‑150 (MQ=60)
                                     AGCAGTTATTACCGTGGTTGCTGGTGCTGTTTGCTGTCAGCGTCATTGCGACCTGGGCTGCGGGCTTCATTAGCCATGTGTCGCTGGTCGAAATCCTGATCCCATTCTGTGTTATGGCGATTGCCAATGGCGCGATCTACCCTATTGTTG                                                                                                                >  SRR19973065.732112/1‑150 (MQ=60)
                                        AGTTATTACCGTGGTTGCTGGTGCTGTTTGCTGTCAGCGTCATTGCGACCTGGGCTGCGGGCTTCATTAGCCATGTGTCGCTGGTCGAAATCCTGATCCCATTCTGTGTTATGGCGATTGCCAATGGCGCGATCTACCCTATTGTTGTCG                                                                                                             >  SRR19973065.1244709/1‑150 (MQ=60)
                                        AGTTATTACCGTGGTTGCTGGTGCTGTTTGCTGTCAGCGTCATTGCGACCTGGGCTGCGGGCTTCATTAGCCATGTGTCGCTGGTCGAAATCCTGATCCCATTCTGTGTTATGGCGATTGCCAATGGCGCGATCTACCCTATTGTTGTCG                                                                                                             <  SRR19973065.2072758/150‑1 (MQ=60)
                                            ATTACCGTGGTTGCTGGTGCTGTTTGCTGTCAGCGTCATTGCGACCTGGGCTGCGGGCTTCATTAGCCATGTGTCGCTGGTCGAAATCCTGATCCCATTCTGTGTTATGGCGATTGCCAATGGCGCGATCTACCCTATTGTTGTCGCCCA                                                                                                         >  SRR19973065.1064271/1‑150 (MQ=60)
                                            ATTACCGTGGTTGCTGGTGCTGTTTGCTGTCAGCGTCATTGCGACCTGGGCTGCGGGCTTCATTAGCCATGTGTCGCTGGTCGAAATCCTGATCCCATTCTGTGTTATGGCGATTGCCAATGGCGCGATCTACCCTATTGTTGTCGCCCA                                                                                                         >  SRR19973065.1295803/1‑150 (MQ=60)
                                            ATTACCGTGGTTGCTGGTGCTGTTTGCTGTCAGCGTCATTGCGACCTGGGCTGCGGGCTTCATTAGCCATGTGTCGCTGGTCGAAATCCTGATCCCATTCTGTGTTATGGCGATTGCCAATGGCGCGATCTACCCTATTGTTGTCGCCCA                                                                                                         >  SRR19973065.3057720/1‑150 (MQ=60)
                                                  GTGGTTGCTGGTGCTGTTTGCTGTCAGCGTCATTGCGACCTGGGCTGCGGGCTTCATTAGCCATGTGTCGCTGGTCGAAATCCTGATCCCATTCTGTGTTATGGCGATTGCCAATGGCGCGATCTACCCTATTGTTGTCGCCCAGGCGCT                                                                                                   <  SRR19973065.61702/150‑1 (MQ=60)
                                                  GTGGTTGCTGGTGCTGTTTGCTGTCAGCGTCATTGCGACCTGGGCTGCGGGCTTCATTAGCCATGTGTCGCTGGTCGAAATCCTGATCCCATTCTGTGTTATGGCGATTGCCAATGGCGCGATCTACCCTATTGTTGTCGCCCAGGCGCT                                                                                                   <  SRR19973065.2597044/150‑1 (MQ=60)
                                                  GTGGTTGCTGGTGCTGTTTGCTGTCAGCGTCATTGCGACCTGGGCTGCGGGCTTCATTAGCCATGTGTCGCTGGTCGAAATCCTGATCCCATTCTGTGTTATGGCGATTGCCAATGGCGCGATCTACCCTATTGTTGTCGCCCAGGCGCT                                                                                                   >  SRR19973065.3446258/1‑150 (MQ=60)
                                                  GTGGTTGCTGGTGCTGTTTGCTGTCAGCGTCATTGCGACCTGGGCTGCGGGCTTCATTAGCCATGTGTCGCTGGTCGAAATCCTGATCCCATTCTGTGTTATGGCGATTGCCAATGGCGCGATCTACCCTATTGTTGTCGCCCAGGCGCT                                                                                                   <  SRR19973065.61299/150‑1 (MQ=60)
                                                     GTTGCTGGTGCTGTTTGCTGTCAGCGTCATTGCGACCTGGGCTGCGGGCTTCATTAGCCATGTGTCGCTGGTCGAAATCCTGATCCCATTCTGTGTTATGGCGATTGCCAATGGCGCGATCTACCCTATTGTTGTCGCCCAGGCGCTGCG                                                                                                <  SRR19973065.1860265/150‑1 (MQ=60)
                                                         CTGGTGCTGTTTGCTGTCAGCGTCATTGCGACCTGGGCTGCGGGCTTCATTAGCCATGTGTCGCTGGTCGAAATCCTGATCCCATTCTGTGTTATGGCGATTGCCAATGGCGCGATCTACCCTATTGTTGTCGCCCAGGCGCTGCGTCCC                                                                                            >  SRR19973065.2497582/1‑150 (MQ=60)
                                                           GGTGCTGTTTGCTGTCAGCGTCATTGCGACCTGGGCTGCGGGCTTCATTAGCCATGTGTCGCTGGTCGAAATCCTGATCCCATTCTGTGTGATGGCGATTGCCAATGGCGCGATCTACCCTATTGTTGTCGCCCAGGCGCTGCGTCCCTT                                                                                          >  SRR19973065.3138044/1‑150 (MQ=60)
                                                           GGTGCTGTTTGCTGTCAGCGTCATTGCGACCTGGGCTGCGGGCTTCATTAGCCATGTGTCGCTGGTCGAAATCCTGATCCCATTCTGTGTGATGGCGATTGCCAATGGCGCGATCTACCCTATTGTTGTCGCCCAGGCGCTGCGTCCCTT                                                                                          >  SRR19973065.2428030/1‑150 (MQ=60)
                                                                   TTGCTGTCAGCGTCATTGCGACCTGGGCTGCGGGCTTCATTAGCCATGTGTCGCTGGTCGAAATCCTGATCCCATTCTGTGTTATGGCGATTGCCAATGGCGCGATCTACCCTATTGTTGTCGCCCAGGCGCTGCGTCCCTTCCCACACG                                                                                  <  SRR19973065.3421301/150‑1 (MQ=60)
                                                                   TTGCTGTCAGCGTCATTGCGACCTGGGCTGCGGGCTTCATTAGCCATGTGTCGCTGGTCGAAATCCTGATCCCATTCTGTGTTATGGCGATTGCCAATGGCGCGATCTACCCTATTGTTGTCGCCCAGGCGCTGCGTCCCTTCCCACACG                                                                                  <  SRR19973065.3421276/150‑1 (MQ=60)
                                                                       TGTCAGCGTCATTGCGACCTGGGCTGCGGGCTTCATTAGCCATGTGTCGCTGGTCGAAATCCTGATCCCATTCTGTGTTATGGCGATTGCCAATGGCGCGATCTACCCTATTGTTGTCGCCCAGGCGCTGCGTCCCTTCCCACACGCAAC                                                                              >  SRR19973065.223576/1‑150 (MQ=60)
                                                                        GTCAGCGTCATTGCGACCTGGGCTGCGGGCTTCATTAGCCATGTGTCGCTGGTCGAAATCCTGATCCCATTCTGTGTTATGGCGATTGCCAATGGCGCGATCTACCCTATTGTTGTCGCCCAGGCGCTGCGTCCCTTCCCACACGCAACT                                                                             <  SRR19973065.2870533/150‑1 (MQ=60)
                                                                        GTCAGCGTCATTGCGACCTGGGCTGCGGGCTTCATTAGCCATGTGTCGCTGGTCGAAATCCTGATCCCATTCTGTGTTATGGCGATTGCCAATGGCGCGATCTACCCTATTGTTGTCGCCCAGGCGCTGCGTCCCTTCCCACACGCAACT                                                                             <  SRR19973065.2332034/150‑1 (MQ=60)
                                                                         TCAGCGTCATTGCGACCTGGGCTGCGGGCTTCATTAGCCATGTGTCGCTGGTCGAAATCCTGATCCCATTCTGTGTTATGGCGATTGCCAATGGCGCGATCTACCCTATTGTTGTCGCCCAGGCGCTGCGTCCCTTCCCACACGCAACTG                                                                            <  SRR19973065.328895/150‑1 (MQ=60)
                                                                         TCAGCGTCATTGCGACCTGGGCTGCGGGCTTCATTAGCCATGTGTCGCTGGTCGAAATCCTGATCCCATTCTGTGTTATGGCGATTGCCAATGGCGCGATCTACCCTATTGTTGTCGCCCAGGCGCTGCGTCCCTTCCCACACGCAACTG                                                                            <  SRR19973065.3109028/150‑1 (MQ=60)
                                                                         TCAGCGTCATTGCGACCTGGGCTGCGGGCTTCATTAGCCATGTGTCGCTGGTCGAAATCCTGATCCCATTCTGTGTTATGGCGATTGCCAATGGCGCGATCTACCCTATTGTTGTCGCCCAGGCGCTGCGTCCCTTCCCACACGCAACTG                                                                            <  SRR19973065.770892/150‑1 (MQ=60)
                                                                            GCGTCATTGCGACCTGGGCTGCGGGCTTCATTAGCCATGTGTCGCTGGTCGAAATCCTGATCCCATTCTGTGTTATGGCGATTGCCAATGGCGCGATCTACCCTATTGTTGTCGCCCAGGCGCTGCGTCCCTTCCCACACGCAACTGGTC                                                                         <  SRR19973065.3223630/150‑1 (MQ=60)
                                                                             CNTCATTGCGACCTGGGCTGCGGGCTTCATTAGCCATGTGTCGCTGGTCGAAATCCTGATCCCATTCTGTGTTATGGCGATTGCCAATGGCGCGATCTACCCTATTGTTGTCGCCCAGGCGCTGCGTCCCTTCCCACACGCAACTGGTCG                                                                        >  SRR19973065.2574575/1‑150 (MQ=60)
                                                                             CGTCATTGCGACCTGGGCTGCGGGCTTCATTAGCCATGTGTCGCTGGTCGAAATCCTGATCCCATTCTGTGTTATGGCGATTGCCAATGGCGCGATCTACCCTATTGTTGTCGCCCAGGCGCTGCGTCCCTTCCCACACGCAACTGGTCG                                                                        >  SRR19973065.951845/1‑150 (MQ=60)
                                                                                   TGCGACCTGGGCTGCGGGCTTCATTAGCCATGTGTCGCTGGTCGAAATCCTGATCCCATTCTGTGTTATGGCGATTGCCAATGGCGCGATCTACCCTATTGTTGTCGCCCAGGCGCTGCGTCCCTTCCCACACGCAACTGGTCGCGCCGC                                                                  >  SRR19973065.1820523/1‑150 (MQ=60)
                                                                                      GACCTGGGCTGCGGGCTTCATTAGCCATGTGTCTCTGGTCGAAATCCTGATCCCATTCTGTGTTATGGCGATTGCCAATGGCGCGATCTACCCTATTGTTGTCGCCCAGGCGCTGCGTCCCTTCCCACACGCAACTGGTCGCGCCGCAGC                                                               >  SRR19973065.2348839/1‑150 (MQ=60)
                                                                                      GACCTGGGCTGCGGGCTTCATTAGCCATGTGTCTCTGGTCGAAATCCTGATCCCATTCTGTGTTATGGCGATTGCCAATGGCGCGATCTACCCTATTGTTGTCGCCCAGGCGCTGCGTCCCTTCCCACACGCAACTGGTCGCGCCGCAGC                                                               >  SRR19973065.1948520/1‑150 (MQ=60)
                                                                                       ACCTGGGCTGCGGGCTTCATTAGCCATGTGTCGCTGGTCGAAATCCTGATCCCATTCTGTGTTATGGCGATTGCCAATGGCGCGATCTACCCTATTGTTGTCGCCCAGGCGCTGCGTCCCTTCCCACACGCAACTGGTCGCGCCGCAGCG                                                              <  SRR19973065.617804/150‑1 (MQ=60)
                                                                                       ACCTGGGCTGCGGGCTTCATTAGCCATGTGTCGCTGGTCGAAATCCTGATCCCATTCTGTGTTATGGCGATTGCCAATGGCGCGATCTACCCTATTGTTGTCGCCCAGGCGCTGCGTCCCTTCCCACACGCAACTGGTCGCGCCGCAGCG                                                              <  SRR19973065.617587/150‑1 (MQ=60)
                                                                                         CTGGGCTGCGGGCTTCATTAGCCATGTGTCGCTGGTCGAAATCCTGATCCCATTCTGTGTTATGGCGATTGCCAATGGCGCGATCTACCCTATTGTTGTCGCCCAGGCGCTGCGTCCCTTCCCACACGCAACTGGTCGCGCCGCAGCGTT                                                            >  SRR19973065.1575420/1‑150 (MQ=60)
                                                                                          TGGGCTGCGGGCTTCATTAGCCATGTGTCGCTGGTCGAAATCCTGATCCCATTCTGTGTTATGGCGATTGCCAATGGCGCGATCTACCCTATTGTTGTCGCCCAGGCGCTGCGTCCCTTCCCACACGCAACTGGTCGCGCCGCAGCGTTG                                                           >  SRR19973065.2851794/1‑150 (MQ=60)
                                                                                          TGGGCTGCGGGCTTCATTAGCCATGTGTCGCTGGTCGAAATCCTGATCCCATTCTGTGTTATGGCGATTGCCAATGGCGCGATCTACCCTATTGTTGTCGCCCAGGCGCTGCGTCCCTTCCCACACGCAACTGGTCGCGCCGCAGCGTTG                                                           >  SRR19973065.808519/1‑150 (MQ=60)
                                                                                          TGGGCTGCGGGCTTCATTAGCCATGTGTCGCTGGTCGAAATCCTGATCCCATTCTGTGTTATGGCGATTGCCAATGGCGCGATCTACCCTATTGTTGTCGCCCAGGCGCTGCGTCCCTTCCCACACGCAACTGGTCGCGCCGCAGCGTTG                                                           <  SRR19973065.287285/150‑1 (MQ=60)
                                                                                          TGGGCTGCGGGCTTCATTAGCCATGTGTCGCTGGTCGAAATCCTGATCCCATTCTGTGTTATGGCGATTGCCAATGGCGCGATCTACCCTATTGTTGTCGCCCAGGCGCTGCGTCCCTTCCCACACGCAACTGGTCGCGCCGCAGCGTTG                                                           <  SRR19973065.286704/150‑1 (MQ=60)
                                                                                          TGGGCTGCGGGCTTCATTAGCCATGTGTCGCTGGTCGAAATCCTGATCCCATTCTGTGTTATGGCGATTGCCAATGGCGCGATCTACCCTATTGTTGTCGCCCAGGCGCTGCGTCCCTTCCCACACGCAACTGGTCGCGCCGCAGCGTTG                                                           >  SRR19973065.2230498/1‑150 (MQ=60)
                                                                                          TGGGCTGCGGGCTTCATTAGCCATGTGTCGCTGGTCGAAATCCTGATCCCATTCTGTGTTATGGCGATTGCCAATGGCGCGATCTACCCTATTGTTGTCGCCCAGGCGCTGCGTCCCTTCCCACACGCAACTGGTCGCGCCGCAGCGTTG                                                           >  SRR19973065.808522/1‑150 (MQ=60)
                                                                                                   GGCTTCATTAGCCATGTGTCGCTGGTCGAAATCCTGATCCCATTCTGTGTTATGGCGATTGCCAATGGCGCGATCTACCCTATTGTTGTCGCCCAGGCGCTGCGTCCCTTCCCACACGCAACTGGTCGCGCCGCAGCGTTGCAGAACACT                                                  <  SRR19973065.1684107/150‑1 (MQ=60)
                                                                                                     CTTCATTAGCCATGTGTCGCTGGTCGAAATCCTGATCCCATTCTGTGTTATGGCGATTGCCAATGGCGCGATCTACCCTATTGTTGTCGCCCAGGCGCTGCGTCCCTTCCCACACGCAACTGGTCGCGCCGCAGCGTTGCAGAACACTCT                                                >  SRR19973065.203685/1‑150 (MQ=60)
                                                                                                      TTCATTAGCCATGTGTCGCTGGTCGAAATCCTGATCCCATTCTGTGTTATGGCGATTGCCAATGGCGCGATCTACCCTATTGTTGTCGCCCAGGCGCTGCGTCCCTTCCCACACGCAACTGGTCGCGCCGCAGCGTTGCAGAACACTCTT                                               >  SRR19973065.1621534/1‑150 (MQ=60)
                                                                                                      TTCATTAGCCATGTGTCGCTGGTCGAAATCCTGATCCCATTCTGTGTTATGGCGATTGCCAATGGCGCGATCTACCCTATTGTTGTCGCCCAGGCGCTGCGTCCCTTCCCACACGCAACTGGTCGCGCCGCAGCGTTGCAGAACACTCTT                                               <  SRR19973065.754760/150‑1 (MQ=60)
                                                                                                           TAGCCATGTGTCGCTGGTCGAAATCCTGATCCCATTCTGTGTTATGGCGATTGCCAATGGCGCGATCTACCCTATTGTTGTCGCCCAGGCGCTGCGTCCCTTCCCACACGCAACTGGTCGCGCCGCAGCGTTGCAGAACACTCTTCAACT                                          <  SRR19973065.13509/150‑1 (MQ=60)
                                                                                                            gtgctcttccgatctGTCGAAATCCTGATCCCATTCTGTGTTATGGCGATTGCCAATGGCGCGATCTACCCTATTGTTGTCGCCCAGGCGCTGCGTCCCTTCCCACACGCAACTGGTCGCGCCGCAGCGTTGCAGAACACTCTTCAACTG                                         <  SRR19973065.249209/135‑1 (MQ=60)
                                                                                                            gtgctcttccgatctGTCGAAATCCTGATCCCATTCTGTGTTATGGCGATTGCCAATGGCGCGATCTACCCTATTGTTGTCGCCCAGGCGCTGCGTCCCTTCCCACACGCAACTGGTCGCGCCGCAGCGTTGCAGAACACTCTTCAACTG                                         <  SRR19973065.2640314/135‑1 (MQ=60)
                                                                                                            gtgctcttccgatctGTCGAAATCCTGATCCCATTCTGTGTTATGGCGATTGCCAATGGCGCGATCTACCCTATTGTTGTCGCCCAGGCGCTGCGTCCCTTCCCACACGCAACTGGTCGCGCCGCAGCGTTGCAGAACACTCTTCAACTG                                         <  SRR19973065.249379/135‑1 (MQ=60)
                                                                                                            AGCCATGTGTCGCTGGTCGAAATCCTGATCCCATTCTGTGTTATGGCGATTGCCAATGGCGCGATCTACCCTATTGTTGTCGCCCAGGCGCTGCGTCCCTTCCCACACGCAACTGGTCGCGCCGCAGCGTTGCAGAACACTCTTCAACTG                                         >  SRR19973065.2422532/1‑150 (MQ=60)
                                                                                                             GCCATGTGTCGCTGGTCGAAATCCTGATCCCATTCTGTGTTATGGCGATTGCCAATGGCGCGATCTACCCTATTGTTGTCGCCCAGGCGCTGCGTCCCTTCCCACACGCAACTGGTCGCGCCGCAGCGTTGCAGAACACTCTTCAACTGG                                        <  SRR19973065.2872902/150‑1 (MQ=60)
                                                                                                             GCCATGTGTCGCTGGTCGAAATCCTGATCCCATTCTGTGTTATGGCGATTGCCAATGGCGCGATCTACCCTATTGTTGTCGCCCAGGCGCTGCGTCCCTTCCCACACGCAACTGGTCGCGCCGCAGCGTTGCAGAACACTCTTCAACTGG                                        <  SRR19973065.2239543/150‑1 (MQ=60)
                                                                                                                ATGTGTCGCTGGTCGAAATCCTGATCCCATTCTGTGTTATGGCGATTGCCAATGGCGCGATCTACCCTATTGTTGTCGCCCAGGCGCTGCGTCCCTTCCCACACGCAACTGGTCGCGCCGCAGCGTTGCAGAACACTCTTCAACTGGGTC                                     <  SRR19973065.729860/150‑1 (MQ=60)
                                                                                                                 TGTGTCGCTGGTCGAAATCCTGATCCCATTCTGTGTTATGGCGATTGCCAATGGCGCGATCTACCCTATTGTTGTCGCCCAGGCGCTGCGTCCCTTCCCACACGCAACTGGTCGCGCCGCAGCGTTGCAGAACACTCTTCAACTGGGTCT                                    >  SRR19973065.3091989/1‑150 (MQ=60)
                                                                                                                      CGCTGGTCGAAATCCTGATCCCATTCTGTGTTATGGCGATTGCCAATGGCGCGATCTACCCTATTGTTGTCGCCCAGGCGCTGCGTCCCTTCCCACACGCAACTGGTCGCGCCGCAGCGTTGCAGAACACTCTTCAACTGGGTCTGTGCT                               <  SRR19973065.2041752/150‑1 (MQ=60)
                                                                                                                         TGGTCGAAATCCTGATCCCATTCTGTGTTATGGCGATTGCCAATGGCGCGATCTACCCTATTGTTGTCGCCCAGGCGCTGCGTCCCTTCCCACACGCAACTGGTCGCGCCGCAGCGTTGCAGAACACTCTTCAACTGGGTCTGTGCTTCC                            >  SRR19973065.1016044/1‑150 (MQ=60)
                                                                                                                         TGGTCGAAATCCTGATCCCATTCTGTGTTATGGCGATTGCCAATGGCGCGATCTACCCTATTGTTGTCGCCCAGGCGCTGCGTCCCTTCCCACACGCAACTGGTCGCGCCGCAGCGTTGCAGAACACTCTTCAACTGGGTCTGTGCTTCC                            >  SRR19973065.1007945/1‑150 (MQ=60)
                                                                                                                         TGGTCGAAATCCTGATCCCATTCTGTGTTATGGCGATTGCCAATGGCGCGATCTACCCTATTGTTGTCGCCCAGGCGCTGCGTCCCTTCCCACACGCAACTGGTCGCGCCGCAGCGTTGCAGAACACTCTTCAACTGGGTCTGTGCTTCC                            <  SRR19973065.2833781/150‑1 (MQ=60)
                                                                                                                              GAAATCCTGATCCCATTCTGTGTTATGGCGATTGCCAATGGCGCGATCTACCCTATTGTTGTCGCCCAGGCGCTGCGTCCCTTCCCACACGCAACTGGTCGCGCCGCAGCGTTGCAGAACACTCTTCAACTGGGTCTGTGCTTCCTCGCA                       >  SRR19973065.63104/1‑150 (MQ=60)
                                                                                                                              GAAATCCTGATCCCATTCTGTGTTATGGCGATTGCCAATGGCGCGATCTACCCTATTGTTGTCGCCCAGGCGCTGCGTCCCTTCCCACACGCAACTGGTCGCGCCGCAGCGTTGCAGAACACTCTTCAACTGGGTCTGTGCTTCCTCGCA                       >  SRR19973065.63100/1‑150 (MQ=60)
                                                                                                                               AAATCCTGATCCCATTCTGTGTTATGGCGATTGCCAATGGCGCGATCTACCCTATTGTTGTCGCCCAGGCGCTGCGTCCCTTCCCACACGCAACTGGTCGCGCCGCAGCGTTGCAGAACACTCTTCAACTGGGTCTGTGCTTCCTCGCAA                      >  SRR19973065.3238400/1‑150 (MQ=60)
                                                                                                                               AAATCCTGATCCCATTCTGTGTTATGGCGATTGCCAATGGCGCGATCTACCCTATTGTTGTCGCCCAGGCGCTGCGTCCCTTCCCACACGCAACTGGTCGCGCCGCAGCGTTGCAGAACACTCTTCAACTGGGTCTGTGCTTCCTCGCAA                      >  SRR19973065.2425395/1‑150 (MQ=60)
                                                                                                                               AAATCCTGATCCCATTCTGTGTTATGGCGATTGCCAATGGCGCGATCTACCCTATTGTTGTCGCCCAGGCGCTGCGTCCCTTCCCACACGCAACTGGTCGCGCCGCAGCGTTGCAGAACACTCTTCAACTGGGTCTGTGCTTCCTCGCAA                      >  SRR19973065.2425778/1‑150 (MQ=60)
                                                                                                                                AATCCTGATCCCATTCTGTGTTATGGCGATTGCCAATGGCGCGATCTACCCTATTGTTGTCGCCCAGGCGCTGCGTCCCTTCCCACACGCAACTGGTCGCGCCGCAGCGTTGCAGAACACTCTTCAACTGGGTCTGTGCTTCCTCGCAAG                     >  SRR19973065.3324480/1‑150 (MQ=60)
                                                                                                                                AATCCTGATCCCATTCTGTGTTATGGCGATTGCCAATGGCGCGATCTACCCTATTGTTGTCGCCCAGGCGCTGCGTCCCTTCCCACACGCAACTGGTCGCGCCGCAGCGTTGCAGAACACTCTTCAACTGGGTCTGTGCTTCCTCGCAAG                     <  SRR19973065.938221/150‑1 (MQ=60)
                                                                                                                                AATCCTGATCCCATTCTGTGTTATGGCGATTGCCAATGGCGCGATCTACCCTATTGTTGTCGCCCAGGCGCTGCGTCCCTTCCCACACGCAACTGGTCGCGCCGCAGCGTTGCAGAACACTCTTCAACTGGGTCTGTGCTTCCTCGCAAG                     >  SRR19973065.663802/1‑150 (MQ=60)
                                                                                                                                AATCCTGATCCCATTCTGTGTTATGGCGATTGCCAATGGCGCGATCTACCCTATTGTTGTCGCCCAGGCGCTGCGTCCCTTCCCACACGCAACTGGTCGCGCCGCAGCGTTGCAGAACACTCTTCAACTGGGTCTGTGCTTCCTCGCAAG                     >  SRR19973065.663767/1‑150 (MQ=60)
                                                                                                                                AATCCTGATCCCATTCTGTGTTATGGCGATTGCCAATGGCGCGATCTACCCTATTGTTGTCGCCCAGGCGCTGCGTCCCTTCCCACACGCAACTGGTCGCGCCGCAGCGTTGCAGAACACTCTTCAACTGGGTCTGTGCTTCCTCGCAAG                     >  SRR19973065.3324572/1‑150 (MQ=60)
                                                                                                                                   CCTGATCCCATTCTGTGTTATGGCGATTGCCAATGGCGCGATCTACCCTATTGTTGTCGCCCAGGCGCTGCGTCCCTTCCCACACGCAACTGGTCGCGCCGCAGCGTTGCAGAACACTCTTCAACTGGGTCTGTGCTTCCTCGCAAGTCT                  <  SRR19973065.2586728/150‑1 (MQ=60)
                                                                                                                                   CCTGATCCCATTCTGTGTTATGGCGATTGCCAATGGCGCGATCTACCCTATTGTTGTCGCCCAGGCGCTGCGTCCCTTCCCACACGCAACTGGTCGCGCCGCAGCGTTGCAGAACACTCTTCAACTGGGTCTGTGCTTCCTCGCAAGTCT                  >  SRR19973065.1680496/1‑150 (MQ=60)
                                                                                                                                      GATCCCATTCTGTGTTATGGCGATTGCCAATGGCGCGATCTACCCTATTGTTGTCGCCCAGGCGCTGCGTCCCTTCCCACACGCAACTGGTCGCGCCGCAGCGTTGCAGAACACTCTTCAACTGGGTCTGTGCTTCCTCGCAAGTCTGGT               >  SRR19973065.2526388/1‑150 (MQ=60)
                                                                                                                                       ATCCCATTCTGTGTTATGGCGATTGCCAATGGCGCGATCTACCCTATTGTTGTCGCCCAGGCGCTGCGTCCCTTCCCACACGCAACTGGTCGCGCCGCAGCGTTGCAGAACACTCTTCAACTGGGTCTGTGCTTCCTCGCAAGTCTGGTA              >  SRR19973065.2909846/1‑150 (MQ=60)
                                                                                                                                       ATCCCATTCTGTGTTATGGCGATTGCCAATGGCGCGATCTACCCTATTGTTGTCGCCCAGGCGCTGCGTCCCTTCCCACACGCAACTGGTCGCGCCGCAGCGTTGCAGAACACTCTTCAACTGGGTCTGTGCTTCCTCGCAAGTCTGGTA              >  SRR19973065.667518/1‑150 (MQ=60)
                                                                                                                                         CCCATTCTGTGTTATGGCGATTGCCAATGGCGCGATCTACCCTATTGTTGTCGCCCAGGCGCTGCGTCCCTTCCCACACGCAACTGGTCGCGCCGCAGCGTTGCAGAACACTCTTCAACTGGGTCTGTGCTTCCTCGCAAGTCTGGTAGT            >  SRR19973065.2849795/1‑150 (MQ=60)
                                                                                                                                         CCCATTCTGTGTTATGGCGATTGCCAATGGCGCGATCTACCCTATTGTTGTCGCCCAGGCGCTGCGTCCCTTCCCACACGCAACTGGTCGCGCCGCAGCGTTGCAGAACACTCTTCAACTGGGTCTGTGCTTCCTCGCAAGTCTGGTAGT            >  SRR19973065.1044627/1‑150 (MQ=60)
                                                                                                                                           CATTCTGTGTTATGGCGATTGCCAATGGCGCGATCTACCCTATTGTTGTCGCCCAGGCGCTGCGTCCCTTCCCACACGCAACTGGTCGCGCCGCAGCGTTGCAGAACACTCTTCAACTGGGTATGTGCTTCCTCGCAAGTCTGGTAGTTT          <  SRR19973065.2368307/150‑1 (MQ=60)
                                                                                                                                            ATTCTGTGTTATGGCGATTGCCAATGGCGCGATCTACCCTATTGTTGTCGCCCAGGCGCTGCGTCCCTTCCCACACGCAACTGGTCGCGCCGCAGCGTTGCAGAACACTCTTCAACTGGGTCTGTGCTTCCTCGCAAGTCTGGTAGTTTC         >  SRR19973065.3268559/1‑150 (MQ=60)
                                                                                                                                              TCTGTGTTATGGCGATTGCCAATGGCGCGATCTACCCTATTGTTGTCGCCCAGGCGCTGCGTCCCTTCCCACACGCAACTGGTCGCGCCGCAGCGTTGCAGAACACTCTTCAACTGGGTCTGTGCTTCCTCGCAAGTCTGGTAGTTTCCT       <  SRR19973065.2881019/150‑1 (MQ=60)
                                                                                                                                              TCTGTGTTATGGCGATTGCCAATGGCGCGATCTACCCTATTGTTGTCGCCCAGGCGCTGCGTCCCTTCCCACACGCAACTGGTCGCGCCGCAGCGTTGCAGAACACTCTTCAACTGGGTCTGTGCTTCCTCGCAAGTCTGGTAGTTTCCT       <  SRR19973065.2305540/150‑1 (MQ=60)
                                                                                                                                              TCTGTGTTATGGCGATTGCCAATGGCGCGATCTACCCTATTGTTGTCGCCCAGGCGCTGCGTCCCTTCCCACACGCAACTGGTCGCGCCGCAGCGTTGCAGAACACTCTTCAACTGGGTCTGTGCTTCCTCGCAAGTCTGGTAGTTTCCT       <  SRR19973065.2305476/150‑1 (MQ=60)
                                                                                                                                                 GTGTTATGGCGATTGCCAATGGCGCGATCTACCCTATTGTTGTCGCCCAGGCGCTGCGTCCCTTCCCACACGCAACTGGTCGCGCCGCAGCGTTGCAGAACACTCTTCAACTGGGTCTGTGCTTCCTCGCAAGTCTGGTAGTTTCCTGGC    <  SRR19973065.293767/150‑1 (MQ=60)
                                                                                                                                                   GTGATGGCGATTGCCAATGGCGCGATCTACCCTATTGTTGTCGCCCAGGCGCTGCGTCCCTTCCCACACGCAACTGGTCGCGCCGCAGCGTTGCAGAACACTCTTCAACTGGGTCTGTGCTTCCTCGCAAGTCTGGTAGTTTCCTGGCTG  <  SRR19973065.3057288/150‑1 (MQ=60)
                                                                                                                                                     |                                                                                                                                                   
TATGGCTGTCGCGCCGCGCTGCAGAAATGGCAAGGCAAGCAGTTATTACCGTGGTTGCTGGTGCTGTTTGCTGTCAGCGTCATTGCGACCTGGGCTGCGGGCTTCATTAGCCATGTGTCGCTGGTCGAAATCCTGATCCCATTCTGTGTGATGGCGATTGCCAATGGCGCGATCTACCCTATTGTTGTCGCCCAGGCGCTGCGTCCCTTCCCACACGCAACTGGTCGCGCCGCAGCGTTGCAGAACACTCTTCAACTGGGTCTGTGCTTCCTCGCAAGTCTGGTAGTTTCCTGGCTG  >  CP009273/1734933‑1735229

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑