Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F434 I0 R1 1130 215.9 7031662 97.1% 6827743 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,944,177 C→A 100% L201L (CTG→CTT)  aspS ← aspartyl‑tRNA synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,944,1770CA100.0% 721.8 / NA 217L201L (CTG→CTT) aspSaspartyl‑tRNA synthetase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (101/116);  total (101/116)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 225 total aligned reads displayed.

CTTCCATCACTTCACGCACTTGCGGCGCGGTCATGAAAGAAGTTTCCACATCGATCTGAGTAAATTCAGGCTGACGGTCAGCACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATCAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCACCAGGTAGTCACGCGCGCCTTCCGGCGTGGCTTTGGTCAGCATCGGAGTTTCGATGTCGAGGAAGCCGTGGTC  >  CP009273/1944032‑1944320
                                                                                                                                                 |                                                                                                                                               
cTTCCATCACTTCACGCACTTGCGGCGCGGTCATGAAAGAAGTTTCCACATCGATCTGAGTAAATTCAGGCTGACGGTCAGCACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATaag                                                                                                                                               >  1:26771/1‑148 (MQ=255)
     aTCACTTCACGCACTTGCGGCGCGGTCATGAAAGAAGTTTCCACATCGATCTGAGTAAATTCAGGCTGACGGTCAGCACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCt                                                                                                                                          <  1:1533019/148‑1 (MQ=255)
       cACTTCACGCACTTGCGGCGCGGTCATGAAAGAAGTTTCCACATCGATCTGAGTAAATTCAGGCTGACGGTCAGCACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGt                                                                                                                                        >  2:2221530/1‑148 (MQ=255)
       aaCTTCACGCACTTGCGGCGCGGTCATGAAAGAAGTTTCCACATCGATCTGAGTAAATTCAGGCTGACGGTCAGCACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAAACGGACATCATAAGCAGCTGt                                                                                                                                        >  1:885759/2‑148 (MQ=255)
        aCTTCACGCACTTGCGGCGCGGTCATGAAAGAAGTTTCCACATCGATCTGAGTAAATTCAGGCTGACGGTCAGCACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGtt                                                                                                                                       <  1:23885/148‑1 (MQ=255)
         cTTCACGCACTTGCTGCGCGGTCATGAAAGAAGTTTCCACATCGATCTGAGTAAATTCAGGCTGACGGTCAGCACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGttt                                                                                                                                      <  2:205848/148‑1 (MQ=255)
          ttCACGCACTTGCGGCGCGGTCATGAAAGAAGTTTCCACATCGATCTGAGTAAATTCAGGCTGACGGTCAGCACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTg                                                                                                                                     >  2:3126317/1‑148 (MQ=255)
               gCACTTGCGGCGCGGTCATGAAAGAAGTTTCCACATCGATCTGAGTAAATTCAGGCTGACGGTCAGCACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGaacaa                                                                                                                                <  2:3367732/148‑1 (MQ=255)
               gCACTTGCGGCGCGGTCATGAAAGAAGTTTCCACATCGATCTGAGTAAATTCAGGCTGACGGTCAGCACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGaacaa                                                                                                                                <  1:802744/148‑1 (MQ=255)
                cACTTGCGGCGCGGTCATGAAAGAAGTTTCCACATCGATCTGAGTAAATTCAGGCTGACGGTCAGCACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGaacaac                                                                                                                               <  2:1367553/148‑1 (MQ=255)
                  cTTGCGGCGCGGTCATGAAAGAAGTTTCCACATCGATCTGAGTAAATTCAGGCTGACGGTCAGCACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTg                                                                                                                             >  2:249256/1‑148 (MQ=255)
                   ttGCGGCGCGGTCATGAAAGAAGTTTCCACATCGATCTGAGTAAATTCAGGCTGACGGTCAGCACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGc                                                                                                                            >  2:1050682/1‑148 (MQ=255)
                   ttGCGGCGCGGTCATGAAAGAAGTTTCCACATCGATCTGAGTAAATTCAGGCTGACGGTCAGCACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGc                                                                                                                            >  2:2803976/1‑148 (MQ=255)
                      cggcgCGGTCATGAAAGAAGTTTCCACATCGATCTGAGTAAATTCAGGCTGACGGTCAGCACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCggg                                                                                                                         >  1:3178789/1‑148 (MQ=255)
                       ggcgCGGTCATGAAAGAAGTTTCCACATCGATCTGAGTAAATTCAGGCTGACGGTCAGCACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCgggg                                                                                                                        <  1:27272/148‑1 (MQ=255)
                        gcgcGGTCATGAAAGAAGTTTCCACATCGATCTGAGTAAATTCAGGCTGACGGTCAGCACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGa                                                                                                                       >  1:3437825/1‑148 (MQ=255)
                         cgcgGTCATGAAAGAAGTTTCCACATCGATCTGAGTAAATTCAGGCTGACGGTCAGCACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGAt                                                                                                                      <  2:558704/148‑1 (MQ=255)
                         cgcgGTCATGAAAGAAGTTTCCACATCGATCTGAGTAAATTCAGGCTGACGGTCAGCACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGAt                                                                                                                      <  2:2172309/148‑1 (MQ=255)
                         cgcgGTCATGAAAGAAGTTTCCACATCGATCTGAGTAAATTCAGGCTGACGGTCAGCACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGAt                                                                                                                      >  1:2945527/1‑148 (MQ=255)
                           cgGTCATGAAAGAAGTTTCCACATCGATCTGAGTAAATTCAGGCTGACGGTCAGCACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTg                                                                                                                    <  1:1215498/148‑1 (MQ=255)
                             gTCATGAAAGAAGTTTCCACATCGATCTGAGTAAATTCAGGCTGACGGTCAGCACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCg                                                                                                                  >  1:724122/1‑148 (MQ=255)
                              tCATGAAAGAAGTTTCCACATCGATCTGAGTAAATTCAGGCTGACGGTCAGCACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCgg                                                                                                                 <  2:1680313/148‑1 (MQ=255)
                               cATGAAAGAAGTTTCCACATCGATCTGAGTAAATTCAGGCTGACGGTCAGCACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGc                                                                                                                <  1:3045172/148‑1 (MQ=255)
                               cATGAAAGAAGTTTCCACATCGATCTGAGTAAATTCAGGCTGACGGTCAGCACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGc                                                                                                                <  1:708047/148‑1 (MQ=255)
                                aTGAAAGAAGTTTCCACATCGATCTGAGTAAATTCAGGCTGACGGTCAGCACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCa                                                                                                               <  2:1839303/148‑1 (MQ=255)
                                  gAAAGAAGTTTCCACATCGATCTGAGTAAATTCAGGCTGACGGTCAGCACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGt                                                                                                             >  1:2444148/1‑148 (MQ=255)
                                     agaagTTTCCACATCGATCTGAGTAAATTCAGGCTGACGGTCAGCACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCg                                                                                                          >  1:2116294/1‑148 (MQ=255)
                                     agaagTTTCCACATCGATCTGAGTAAATTCAGGCTGACGGTCAGCACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCg                                                                                                          >  2:1474101/1‑148 (MQ=255)
                                       aagTTTCCACATCGATCTGAGTAAATTCAGGCTGACGGTCAGCACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTa                                                                                                        >  2:2845415/1‑148 (MQ=255)
                                        agTTTCCACATCGATCTGAGTAAATTCAGGCTGACGGTCAGCACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAg                                                                                                       >  2:1493400/1‑148 (MQ=255)
                                          tttCCACATCGATCTGAGTAAATTCAGGCTGACGGTCAGCACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGaa                                                                                                     >  2:876991/1‑148 (MQ=255)
                                           ttCCACATCGATCTGAGTAAATTCAGGCTGACGGTCAGCACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAAt                                                                                                    <  1:3121123/148‑1 (MQ=255)
                                           ttCCACATCGATCTGAGTAAATTCAGGCTGACGGTCAGCACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAAt                                                                                                    <  1:2502071/148‑1 (MQ=255)
                                              cacaTCGATCTGAGTAAATTCAGGCTGACGGTCAGCACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTa                                                                                                 <  1:672627/148‑1 (MQ=255)
                                               acaTCGATCTGAGTAAATTCAGGCTGACGGTCAGCACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGGAGAATTTAc                                                                                                >  2:2085472/1‑148 (MQ=255)
                                                   cGATCTGAGTAAATTCAGGCTGACGGTCAGCACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCttt                                                                                            >  2:522981/1‑148 (MQ=255)
                                                    gATCTGAGTAAATTCAGGCTGACGGTCAGCACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTtg                                                                                           <  2:1745128/148‑1 (MQ=255)
                                                     aTCTGAGTAAATTCAGTCTGACGGTCAGCACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTtgt                                                                                          <  1:93014/148‑1 (MQ=255)
                                                      tCTGAGTAAATTCAGGCTGACGGTCAGCACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTtttg                                                                                         <  1:1287949/148‑1 (MQ=255)
                                                      tCTGAGTAAATTCAGGCTGACGGTCAGCACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTtgtg                                                                                         >  1:1702272/1‑148 (MQ=255)
                                                      tCTGAGTAAATTCAGGCTGACGGTCAGCACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTtgtg                                                                                         >  2:755401/1‑148 (MQ=255)
                                                         gAGTAAATTCAGGCTGACGGTCAGCACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGcac                                                                                      <  1:1749539/148‑1 (MQ=255)
                                                         gAGTAAATTCAGGCTGACGGTCAGCACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGcac                                                                                      >  1:2020344/1‑148 (MQ=255)
                                                         gAGTAAATTCAGGCTGACGGTCAGCACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGcac                                                                                      <  1:3485663/148‑1 (MQ=255)
                                                         gAGCAACTTCAGGCTGACGGTCAGCACGCACGTCTTCGTCACGGAAGCATTTAACGATCTGCTAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGcac                                                                                      <  2:2929283/148‑1 (MQ=255)
                                                             aaaTTCTGGCTGACGGTCAGCACGCAGGTCTTCGTCACGGAAGCTTTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACga                                                                                  <  1:677305/148‑1 (MQ=255)
                                                               aTTCAGGCTGACGGTCAGCACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACgaga                                                                                >  2:436514/1‑148 (MQ=255)
                                                                ttCAGGCTGACGGTCAGCACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGaa                                                                               >  2:349635/1‑148 (MQ=255)
                                                                ttCAGGCTGACGGTCAGCACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGaa                                                                               >  2:2652300/1‑148 (MQ=255)
                                                                ttCAGGCTGACGGTCAGCACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGaa                                                                               <  1:1851442/148‑1 (MQ=255)
                                                                ttCAGGCTGACGGTCAGCACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGaa                                                                               >  1:2264057/1‑148 (MQ=255)
                                                                  cAGGCTGACGGTCAGCACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAgg                                                                             <  2:1703456/148‑1 (MQ=255)
                                                                  cAGGCTGACGGTCAGCACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAgg                                                                             >  2:2698349/1‑148 (MQ=255)
                                                                   aGGCTGACGGTCAGCACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGc                                                                            <  1:1470022/148‑1 (MQ=255)
                                                                      cTGACGGTCAGCACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCAcc                                                                         <  1:2701595/148‑1 (MQ=255)
                                                                       tGACGGTCAGCACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTATCGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCACCa                                                                        <  1:2758023/148‑1 (MQ=255)
                                                                       tGACGGTCAGCACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCACCa                                                                        <  2:885760/148‑1 (MQ=255)
                                                                       tGACGGTCAGCACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCACCa                                                                        <  1:2354648/148‑1 (MQ=255)
                                                                        gACGGTCAGCACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCACCAg                                                                       >  2:418996/1‑148 (MQ=255)
                                                                         aCGGTCAGCACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCACCAgg                                                                      <  2:3079140/148‑1 (MQ=255)
                                                                              cAGCACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAGTTTACCTTTGTGCACACGAGAAGGCACCAGGTAGTc                                                                 <  2:2263894/148‑1 (MQ=255)
                                                                              cAGCACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCACCAGGTAGTc                                                                 <  2:3226391/148‑1 (MQ=255)
                                                                               aGCACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCACCAGGTAGTCa                                                                <  1:1248016/148‑1 (MQ=255)
                                                                                 cACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCACCAGGTAGTCAcg                                                              >  2:3060350/1‑148 (MQ=255)
                                                                                 cACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCACCAGGTAGTCAcg                                                              >  1:2232640/1‑148 (MQ=255)
                                                                                 cACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCACCAGGTAGTCAcg                                                              >  2:1307836/1‑148 (MQ=255)
                                                                                  aCGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCACCAGGTAGTCAcgc                                                             <  2:359146/148‑1 (MQ=255)
                                                                                       ggTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCACCAGGTAGTCACGCGCGcc                                                        <  2:26771/148‑1 (MQ=255)
                                                                                        gTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCACCAGGTAGTCACGCGCGCCt                                                       <  2:3498116/148‑1 (MQ=255)
                                                                                             cGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCACCAGGTAGTCACGCGCGCCTTCCgg                                                  <  1:66046/148‑1 (MQ=255)
                                                                                               tCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCACCAGGTAGTCACGCGCGCCTTCCGGCg                                                <  1:343999/148‑1 (MQ=255)
                                                                                               tCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCACCAGGTAGTCACGCGCGCCTTCCGGCg                                                <  1:3268419/148‑1 (MQ=255)
                                                                                               tCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCACCAGGTAGTCACGCGCGCCTTCCGGCg                                                <  1:2469259/148‑1 (MQ=255)
                                                                                                   ggAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCACCAGGTAGTCACGCGCGCCTTCCGGCGTGGc                                            >  2:2895331/1‑148 (MQ=255)
                                                                                                    gAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTCGAATTTACCTTTGTGCACACGAGAAGGCACCAGGTCGTCACGCGCGCCTTCCGGCGTGGCt                                           >  2:3055457/1‑148 (MQ=255)
                                                                                                    gAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCACCAGGTAGTCACGCGCGCCTTCCGGCGTGGCt                                           >  1:743361/1‑148 (MQ=255)
                                                                                                     aaGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCACCAGGTAGTCACGCGCGCCTTCCGGCGTGGCtt                                          >  1:3147147/1‑148 (MQ=255)
                                                                                                        cATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCACCAGGTAGTCACGCGCGCCTTCCGGCGTGGCTTTgg                                       <  1:138671/148‑1 (MQ=255)
                                                                                                              aCGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCACCAGGTAGTCACGCGCGCCTTCCGGCGTGGCTTTGGTCAGCa                                 >  2:1962179/1‑148 (MQ=255)
                                                                                                                 aTCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCACCAGGTAGTCACGCGCGCCTTCCGGCGTGGCTTTGGTCAGCATCg                              >  1:1003595/1‑148 (MQ=255)
                                                                                                                 aTCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCACCAGGTAGTCACGCGCGCCTTCCGGCGTGGCTTTGGTCAGCATCg                              <  2:1238443/148‑1 (MQ=255)
                                                                                                                  tCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCACCAGGTAGTCACGCGCGCCTTCCGGCGTGGCTTTGGTCAGCATCgg                             >  2:1158231/1‑148 (MQ=255)
                                                                                                                   cTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCACCAGGTAGTCACGCGCGCCTTCCGGCGTGGCTTTGGTCAGCATCGGa                            >  1:2943974/1‑148 (MQ=255)
                                                                                                                     gATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCACCAGGTAGTCACGCGCGCCTTCCGGCGTGGCTTTGGTCAGCATCGGAGt                          <  2:1600438/148‑1 (MQ=255)
                                                                                                                           aaCGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCACCAGGTAGTCACGCGCGCCTTCCGGCGTGGCTTTGGTCAGCATCGGAGTTTCGAt                    <  2:3178786/148‑1 (MQ=255)
                                                                                                                           aaCGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCACCAGGTAGTCACGCGCGCCTTCCGGCGTGGCTTTGGTCAGCATCGGAGTTTCGAt                    <  1:807965/148‑1 (MQ=255)
                                                                                                                            aCGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCACCAGGTAGTCACGCGCGCCTTCCGGCGTGGCTTTGGTCAGCATCGGAGTTTCGATg                   <  1:330600/148‑1 (MQ=255)
                                                                                                                             cGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCACCAGGTAGTCACGCGCGCCTTCCGGCGTGGCTTTGGTCAGCATCGGAGTTTCGATGt                  <  2:3147147/148‑1 (MQ=255)
                                                                                                                                tCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCACCAGGTAGTCACGCGCGCCTTCCGGCGTGGCTTTGGTCAGCATCGGAGTTTCGATGTCGa               >  2:2761152/1‑148 (MQ=255)
                                                                                                                                tCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCACCAGGTAGTCACGCGCGCCTTCCGGCGTGGCTTTGGTCAGCATCGGAGTTTCGATGTCGa               >  2:850999/1‑148 (MQ=255)
                                                                                                                                tCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCACCAGGTAGTCACGCGCGCCTTCCGGCGTGGCTTTGGTCAGCATCGGAGTTTCGATGTCGa               <  2:1256205/148‑1 (MQ=255)
                                                                                                                                 cAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCACCAGGTAGTCACGCGCGCCTTCCGGCGTGGCTTTGGTCAGCATCGGAGTTTCGATGTCGAg              >  1:847096/1‑148 (MQ=255)
                                                                                                                                  aaaaCCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCACCAGGTAGTCACGCGCGCCTTCCGGCGTGGCTTTGGTCAGCATCGGAGTTTCGATGTCGAgg             >  2:1144874/1‑148 (MQ=255)
                                                                                                                                  aaaaCCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCACCAGGTAGTCACGCGCGCCTTCCGGCGTGGCTTTGGTCAGCATCGGAGTTTCGATGTCGAgg             <  2:1795792/148‑1 (MQ=255)
                                                                                                                                   aaaCCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCACCAGGTAGTCACGCGCGCCTTCCGGCGTGGCTGTGGTCAGCATCGGAGTTTCGATGTCGAGGa            >  1:2219161/1‑148 (MQ=255)
                                                                                                                                     aCCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCACCAGGTAGTCACGCGCGCCTTCCGGCGTGGCTTTGGTCAGCATCGGAGTTTCGATGTCGAGGAAg          <  2:1328447/148‑1 (MQ=255)
                                                                                                                                     aCCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCACCAGGTAGTCACGCGCGCCTTCCGGCGTGGCTTTGGTCAGCATCGGAGTTTCGATGTCGAGGAAg          >  1:1047579/1‑148 (MQ=255)
                                                                                                                                      ccGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCACCAGGTAGTCACGCGCGCCTTCCGGCGTGGCTTTGGTCAGCATCGGAGTTTCGATGTCGAGGAAGc         >  2:206788/1‑148 (MQ=255)
                                                                                                                                       cGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCACCAGGTAGTCACGCGCGCCTTCCGGCGTGGCTTTGGTCAGCATCGGAGTTTCGATGTCGAGGAAGcc        >  1:886680/1‑148 (MQ=255)
                                                                                                                                             tcataaGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCACCAGGTAGTCACGCGCGCCTTCCGGCGTGGCTTTGGTCAGCATCGGAGTTTCGATGTCGAGGAAGCCGTGGTc  <  1:484507/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                               
CTTCCATCACTTCACGCACTTGCGGCGCGGTCATGAAAGAAGTTTCCACATCGATCTGAGTAAATTCAGGCTGACGGTCAGCACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATCAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCACCAGGTAGTCACGCGCGCCTTCCGGCGTGGCTTTGGTCAGCATCGGAGTTTCGATGTCGAGGAAGCCGTGGTC  >  CP009273/1944032‑1944320

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CGCTTCCATCACTTCACGCACTTGCGGCGCGGTCATGAAAGAAGTTTCCACATCGATCTGAGTAAATTCAGGCTGACGGTCAGCACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATCAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCACCAGGTAGTCACGCGCGCCTTCCGGCGTGGCTTTGGTCAGCATCGGAGTTTCGATGTCGAGGAAGCCGTGGTCATC  >  CP009273/1944030‑1944323
                                                                                                                                                   |                                                                                                                                                  
CGCTTCCATCACTTCACGCACTTGCGGCGCGGTCATGAAAGAAGTTTCCACATCGATCTGAGTAAATTCAGGCTGACGGTCAGCACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAG                                                                                                                                                  >  SRR19973065.27124/1‑150 (MQ=60)
 GCTTCCATCACTTCACGCACTTGCGGCGCGGTCATGAAAGAAGTTTCCACATCGATCTGAGTAAATTCAGGCTGACGGTCAGCACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGC                                                                                                                                                 <  SRR19973065.2185784/150‑1 (MQ=60)
 GCTTCCATCACTTCACGCACTTGCGGCGCGGTCATGAAAGAAGTTTCCACATCGATCTGAGTAAATTCAGGCTGACGGTCAGCACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGC                                                                                                                                                 <  SRR19973065.1206685/150‑1 (MQ=60)
  CTTCCATCACTTCACGCACTTGCGGCGCGGTCATGAAAGAAGTTTCCACATCGATCTGAGTAAATTCAGGCTGACGGTCAGCACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCA                                                                                                                                                >  SRR19973065.1264477/1‑150 (MQ=60)
     CCATCACTTCACGCACTTGCGGCGCGGTCATGAAAGAAGTTTCCACATCGATCTGAGTAAATTCAGGCTGACGGTCAGCACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCT                                                                                                                                             <  SRR19973065.711202/150‑1 (MQ=60)
       ATCACTTCACGCACTTGCGGCGCGGTCATGAAAGAAGTTTCCACATCGATCTGAGTAAATTCAGGCTGACGGTCAGCACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGT                                                                                                                                           >  SRR19973065.891390/1‑150 (MQ=60)
       ATCACTTCACGCACTTGCGGCGCGGTCATGAAAGAAGTTTCCACATCGATCTGAGTAAATTCAGGCTGACGGTCAGCACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGT                                                                                                                                           <  SRR19973065.1543122/150‑1 (MQ=60)
       ATAACTTCACGCACTTGCGGCGCGGTCATGAAAGAAGTTTCCACATCGATCTGAGTAAATTCAGGCTGACGGTCAGCACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAAACGGACATCATAAGCAGCTGT                                                                                                                                           >  SRR19973065.891389/1‑150 (MQ=60)
          ACTTCACGCACTTGCGGCGCGGTCATGAAAGAAGTTTCCACATCGATCTGAGTAAATTCAGGCTGACGGTCAGCACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTG                                                                                                                                        <  SRR19973065.24204/150‑1 (MQ=60)
          ACTTCACGCACTTGCGGCGCGGTCATGAAAGAAGTTTCCACATCGATCTGAGTAAATTCAGGCTGACGGTCAGCACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTG                                                                                                                                        <  SRR19973065.684601/150‑1 (MQ=60)
                 GCACTTGCGGCGCGGTCATGAAAGAAGTTTCCACATCGATCTGAGTAAATTCAGGCTGACGGTCAGCACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACT                                                                                                                                 <  SRR19973065.808005/150‑1 (MQ=60)
                 GCACTTGCGGCGCGGTCATGAAAGAAGTTTCCACATCGATCTGAGTAAATTCAGGCTGACGGTCAGCACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACT                                                                                                                                 <  SRR19973065.141225/150‑1 (MQ=60)
                 GCACTTGCGGCGCGGTCATGAAAGAAGTTTCCACATCGATCTGAGTAAATTCAGGCTGACGGTCAGCACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACT                                                                                                                                 <  SRR19973065.808050/150‑1 (MQ=60)
                      gtgtgctcttccgatctAGAAGTTTCCACATCGATCTGAGTAAATTCAGGCTGACGGTCAGCACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGG                                                                                                                            <  SRR19973065.97709/133‑1 (MQ=60)
                      TGCGGCGCGGTCATGAAAGAAGTTTCCACATCGATCTGAGTAAATTCAGGCTGACGGTCAGCACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGG                                                                                                                            >  SRR19973065.3197997/1‑150 (MQ=60)
                      TGCGGCGCGGTCATGAAAGAAGTTTCCACATCGATCTGAGTAAATTCAGGCTGACGGTCAGCACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGG                                                                                                                            >  SRR19973065.3198000/1‑150 (MQ=60)
                        CGGCGCGGTCATGAAAGAAGTTTCCACATCGATCTGAGTAAATTCAGGCTGACGGTCAGCACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGA                                                                                                                          >  SRR19973065.3458796/1‑150 (MQ=60)
                        CGGCGCGGTCATGAAAGAAGTTTCCACATCGATCTGAGTAAATTCAGGCTGACGGTCAGCACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGA                                                                                                                          >  SRR19973065.3458802/1‑150 (MQ=60)
                         GGCGCGGTCATGAAAGAAGTTTCCACATCGATCTGAGTAAATTCAGGCTGACGGTCAGCACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGATATCATAAGCAGCTGTTTGAACAACTGCGGGGAT                                                                                                                         >  SRR19973065.2963409/1‑150 (MQ=60)
                         GGCGCGGTCATGAAAGAAGTTTCCACATCGATCTGAGTAAATTCAGGCTGACGGTCAGCACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGAT                                                                                                                         >  SRR19973065.2963433/1‑150 (MQ=60)
                         GGCGCGGTCATGAAAGAAGTTTCCACATCGATCTGAGTAAATTCAGGCTGACGGTCAGCACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGAT                                                                                                                         <  SRR19973065.27632/150‑1 (MQ=60)
                         GGCGCGGTCATGAAAGAAGTTTCCACATCGATCTGAGTAAATTCAGGCTGACGGTCAGCACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGAT                                                                                                                         <  SRR19973065.2346118/150‑1 (MQ=60)
                             CGGTCATGAAAGAAGTTTCCACATCGATCTGAGTAAATTCAGGCTGACGGTCAGCACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCG                                                                                                                     >  SRR19973065.728970/1‑150 (MQ=60)
                             CGGTCATGAAAGAAGTTTCCACATCGATCTGAGTAAATTCAGGCTGACGGTCAGCACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCG                                                                                                                     <  SRR19973065.1223374/150‑1 (MQ=60)
                                 CATGAAAGAAGTTTCCACATCGATCTGAGTAAATTCAGGCTGACGGTCAGCACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAG                                                                                                                 <  SRR19973065.3063711/150‑1 (MQ=60)
                                 CATGAAAGAAGTTTCCACATCGATCTGAGTAAATTCAGGCTGACGGTCAGCACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAG                                                                                                                 <  SRR19973065.3063807/150‑1 (MQ=60)
                                 CATGAAAGAAGTTTCCACATCGATCTGAGTAAATTCAGGCTGACGGTCAGCACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAG                                                                                                                 <  SRR19973065.712771/150‑1 (MQ=60)
                                  ATGAAAGAAGTTTCCACATCGATCTGAGTAAATTCAGGCTGACGGTCAGCACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGT                                                                                                                >  SRR19973065.2460239/1‑150 (MQ=60)
                                  ATGAAAGAAGTTTCCACATCGATCTGAGTAAATTCAGGCTGACGGTCAGCACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGT                                                                                                                >  SRR19973065.2459549/1‑150 (MQ=60)
                                     AAAGAAGTTTCCACATCGATCTGAGTAAATTCAGGCTGACGGTCAGCACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCG                                                                                                             >  SRR19973065.2129915/1‑150 (MQ=60)
                                     AAAGAAGTTTCCACATCGATCTGAGTAAATTCAGGCTGACGGTCAGCACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCG                                                                                                             >  SRR19973065.2130363/1‑150 (MQ=60)
                                       AGAAGTTTCCACATCGATCTGAGTAAATTCAGGCTGACGGTCAGCACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTA                                                                                                           <  SRR19973065.453203/150‑1 (MQ=60)
                                          AGTTTCCACATCGATCTGAGTAAATTCAGGCTGACGGTCAGCACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAA                                                                                                        <  SRR19973065.2515880/150‑1 (MQ=60)
                                           GTTTCCACATCGATCTGAGTAAATTCAGGCTGACGGTCAGCACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAAT                                                                                                       <  SRR19973065.2646937/150‑1 (MQ=60)
                                             TTCCACATCGATCTGAGTAAATTCAGGCTGACGGTCAGCACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTT                                                                                                     <  SRR19973065.3140209/150‑1 (MQ=60)
                                             TTCCACATCGATCTGAGTAAATTCAGGCTGACGGTCAGCACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTT                                                                                                     <  SRR19973065.2517797/150‑1 (MQ=60)
                                             TTCCACATCGATCTGAGTAAATTCAGGCTGACGGTCAGCACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTT                                                                                                     <  SRR19973065.3140133/150‑1 (MQ=60)
                                               CCACATCGATCTGAGTAAATTCAGGCTGACGGTCAGCACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTAC                                                                                                   <  SRR19973065.2033855/150‑1 (MQ=60)
                                                CACATCGATCTGAGTAAATTCAGGCTGACGGTCAGCACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACC                                                                                                  <  SRR19973065.676568/150‑1 (MQ=60)
                                                CACATCGATCTGAGTAAATTCAGGCTGACGGTCAGCACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACC                                                                                                  <  SRR19973065.677107/150‑1 (MQ=60)
                                                CACATCGATCTGAGTAAATTCAGGCTGACGGTCAGCACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACC                                                                                                  <  SRR19973065.677198/150‑1 (MQ=60)
                                                   ATCGATCTGAGTAAATTCAGGCTGACGGTCAGCACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTT                                                                                               >  SRR19973065.478314/1‑150 (MQ=60)
                                                      GATCTGAGTAAATTCAGGCTGACGGTCAGCACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTG                                                                                            >  SRR19973065.1713603/1‑150 (MQ=60)
                                                      GATCTGAGTAAATTCAGGCTGACGGTCAGCACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTG                                                                                            >  SRR19973065.1713613/1‑150 (MQ=60)
                                                      GATCTGAGTAAATTCAGGCTGACGGTCAGCACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTG                                                                                            <  SRR19973065.1273870/150‑1 (MQ=60)
                                                       ATCTGAGTAAATTCAGTCTGACGGTCAGCACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGC                                                                                           <  SRR19973065.93893/150‑1 (MQ=60)
                                                       ATCTGAGTAAATTCAGGCTGACGGTCAGCACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGC                                                                                           <  SRR19973065.93860/150‑1 (MQ=60)
                                                        TCTGAGTAAATTCAGGCTGACGGTCAGCACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTTTGCA                                                                                          <  SRR19973065.1296441/150‑1 (MQ=60)
                                                         CTGAGTAAATTCAGGCTGACGGTCAGCACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCAC                                                                                         >  SRR19973065.2033346/1‑150 (MQ=60)
                                                           GAGTAAATTCAGGCTGACGGTCAGCACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACAC                                                                                       <  SRR19973065.1761064/150‑1 (MQ=60)
                                                           GAGTAAATTCAGGCTGACGGTCAGCACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACAC                                                                                       <  SRR19973065.3506865/150‑1 (MQ=60)
                                                              TAAATTCAGGCTGACCGTCAGCACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAG                                                                                    >  SRR19973065.2014459/1‑150 (MQ=60)
                                                               AAATTCTGGCTGACGGTCAGCACGCAGGTCTTCGTCACGGAAGCTTTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGA                                                                                   <  SRR19973065.681823/150‑1 (MQ=60)
                                                                AATTCAGGCTGACGGTCAGCACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAA                                                                                  >  SRR19973065.2278368/1‑150 (MQ=60)
                                                                  TTCAGGCTGACGGTCAGCACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGG                                                                                <  SRR19973065.1863639/150‑1 (MQ=60)
                                                                  TTCAGGCTGACGGTCAGCACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGG                                                                                <  SRR19973065.925338/150‑1 (MQ=60)
                                                                  TTCAGGCTGACGGTCAGCACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGG                                                                                <  SRR19973065.1500594/150‑1 (MQ=60)
                                                                   TCAGGCTGACGGTCAGCACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGC                                                                               >  SRR19973065.808145/1‑150 (MQ=60)
                                                                     AGGCTGACGGTCAGCACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCAC                                                                             <  SRR19973065.251009/150‑1 (MQ=60)
                                                                     AGGCTGACGGTCAGCACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCAC                                                                             <  SRR19973065.2872436/150‑1 (MQ=60)
                                                                     AGGCTGACGGTCAGCACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCAC                                                                             <  SRR19973065.1479444/150‑1 (MQ=60)
                                                                        CTGACGGTCAGCACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCACCAG                                                                          <  SRR19973065.2718386/150‑1 (MQ=60)
                                                                         TGACGGTCAGCACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTATCGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCACCAGG                                                                         <  SRR19973065.2775173/150‑1 (MQ=60)
                                                                         TGACGGTCAGCACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCACCAGG                                                                         <  SRR19973065.2775110/150‑1 (MQ=60)
                                                                         TGACGGTCAGCACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCACCAGG                                                                         <  SRR19973065.2369292/150‑1 (MQ=60)
                                                                         TGACGGTCAGCACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCACCAGG                                                                         <  SRR19973065.1721543/150‑1 (MQ=60)
                                                                            CGGTCAGCACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCACCAGGTAG                                                                      >  SRR19973065.863360/1‑150 (MQ=60)
                                                                                 AGCACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCACCAGGTAGTCACG                                                                 >  SRR19973065.2246892/1‑150 (MQ=60)
                                                                                 AGCACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCACCAGGTAGTCACG                                                                 <  SRR19973065.1256237/150‑1 (MQ=60)
                                                                                 AGCACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCACCAGGTAGTCACG                                                                 >  SRR19973065.2246846/1‑150 (MQ=60)
                                                                                    ACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCACCAGGTAGTCACGCGC                                                              >  SRR19973065.2291904/1‑150 (MQ=60)
                                                                                       CAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCACCAGGTAGTCACGCGCGCC                                                           >  SRR19973065.3118739/1‑150 (MQ=60)
                                                                                          GTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCACCAGGTAGTCACGCGCGCCTTC                                                        >  SRR19973065.743423/1‑150 (MQ=60)
                                                                                               CGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCACCAGGTAGTCACGCGCGCCTTCCGGCG                                                   <  SRR19973065.66753/150‑1 (MQ=60)
                                                                                               CGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCACCAGGTAGTCACGCGCGCCTTCCGGCG                                                   <  SRR19973065.2391677/150‑1 (MQ=60)
                                                                                                 TCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCACCAGGTAGTCACGCGCGCCTTCCGGCGTG                                                 <  SRR19973065.3288277/150‑1 (MQ=60)
                                                                                                 TCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCACCAGGTAGTCACGCGCGCCTTCCGGCGTG                                                 <  SRR19973065.3288242/150‑1 (MQ=60)
                                                                                                 TCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCACCAGGTAGTCACGCGCGCCTTCCGGCGTG                                                 <  SRR19973065.2484810/150‑1 (MQ=60)
                                                                                                 TCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCACCAGGTAGTCACGCGCGCCTTCCGGCGTG                                                 <  SRR19973065.713432/150‑1 (MQ=60)
                                                                                                 TCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCACCAGGTAGTCACGCGCGCCTTCCGGCGTG                                                 <  SRR19973065.346406/150‑1 (MQ=60)
                                                                                                 TCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCACCAGGTAGTCACGCGCGCCTTCCGGCGTG                                                 <  SRR19973065.3288086/150‑1 (MQ=60)
                                                                                                    CGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCACCAGGTAGTCACGCGCGCCTTCCGGCGTGGCT                                              >  SRR19973065.748341/1‑150 (MQ=60)
                                                                                                     GGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCACCAGGTAGTCACGCGCGCCTTCCGGCGTGGCTT                                             <  SRR19973065.3465874/150‑1 (MQ=60)
                                                                                                     GGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCACCAGGTAGTCACGCGCGCCTTCCGGCGTGGCTT                                             >  SRR19973065.3166259/1‑150 (MQ=60)
                                                                                                       AAGCATTTAGCGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCACCAGGTAGTCACGCGCGCCTTCCGGCGTGGCTTTG                                           >  SRR19973065.273706/1‑150 (MQ=60)
                                                                                                       AAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCACCAGGTAGTCACGCGCGCCTTCCGGCGTGGCTTTG                                           >  SRR19973065.1079446/1‑150 (MQ=60)
                                                                                                          CATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCACCAGGTAGTCACGCGCGCCTTCCGGCGTGGCTTTGGTC                                        <  SRR19973065.139859/150‑1 (MQ=60)
                                                                                                           ATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCACCAGGTAGTCACGCGCGCCTTCCGGCGTGGCTTTGGTCA                                       <  SRR19973065.3106745/150‑1 (MQ=60)
                                                                                                                 CGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCACCAGGTAGTCACGCGCGCCTTCCGGCGTGGCTTTGGTCAGCATCG                                 >  SRR19973065.1010580/1‑150 (MQ=60)
                                                                                                                 CGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCACCAGGTAGTCACGCGCGCCTTCCGGCGTGGCTTTGGTCAGCATCG                                 >  SRR19973065.1010261/1‑150 (MQ=60)
                                                                                                                  GATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCACCAGGTAGTCACGCGCGCCTTCCGGCGTGGCTTTGGTCAGCATCGG                                >  SRR19973065.1807579/1‑150 (MQ=60)
                                                                                                                   ATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCACCAGGTAGTCACGCGCGCCTTCCGGCGTGGCTTTGGTCAGCATCGGA                               >  SRR19973065.2961859/1‑150 (MQ=60)
                                                                                                                     CTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCACCAGGTAGTCACGCGCGCCTTCCGGCGTGGCTTTGGTCAGCATCGGAGT                             >  SRR19973065.1380857/1‑150 (MQ=60)
                                                                                                                      TGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCACCAGGTAGTCACGCGCGCCTTCCGGCGTGGCTTTGGTCAGCATCGGAGTT                            <  SRR19973065.2233747/150‑1 (MQ=60)
                                                                                                                             AACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCACCAGGTAGTCACGCGCGCCTTCCGGCGTGGCTTTGGTCAGCATCGGAGTTTCGATGT                     <  SRR19973065.812917/150‑1 (MQ=60)
                                                                                                                             AACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCACCAGGTAGTCACGCGCGCCTTCCGGCGTGGCTTTGGTCAGCATCGGAGTTTCGATGT                     <  SRR19973065.813299/150‑1 (MQ=60)
                                                                                                                              ACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCACCAGGTAGTCACGCGCGCCTTCCGGCGTGGCTTTGGTCAGCATCGGAGTTTCGATGTC                    <  SRR19973065.1256166/150‑1 (MQ=60)
                                                                                                                              ACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCACCAGGTAGTCACGCGCGCCTTCCGGCGTGGCTTTGGTCAGCATCGGAGTTTCGATGTC                    <  SRR19973065.332913/150‑1 (MQ=60)
                                                                                                                              ACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCACCAGGTAGTCACGCGCGCCTTCCGGCGTGGCTTTGGTCAGCATCGGAGTTTCGATGTC                    <  SRR19973065.332912/150‑1 (MQ=60)
                                                                                                                                 GTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCACCAGGTAGTCACGCGCGCCTTCCGGCGTGGCTTTGGTCAGCATCGGAGTTTCGATGTCGAG                 >  SRR19973065.852567/1‑150 (MQ=60)
                                                                                                                                 GTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCACCAGGTAGTCACGCGCGCCTTCCGGCGTGGCTTTGGTCAGCATCGGAGTTTCGATGTCGAG                 >  SRR19973065.852586/1‑150 (MQ=60)
                                                                                                                                   CAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCACCAGGTAGTCACGCGCGCCTTCCGGCGTGGCTTTGGTCAGCATCGGAGTTTCGATGTCGAGGA               <  SRR19973065.188005/150‑1 (MQ=60)
                                                                                                                                   CAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCACCAGGTAGTCACGCGCGCCTTCCGGCGTGGCTTTGGTCAGCATCGGAGTTTCGATGTCGAGGA               >  SRR19973065.1619421/1‑150 (MQ=60)
                                                                                                                                   CAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCACCAGGTAGTCACGCGCGCCTTCCGGCGTGGCTTTGGTCAGCATCGGAGTTTCGATGTCGAGGA               >  SRR19973065.632751/1‑150 (MQ=60)
                                                                                                                                   CAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCACCAGGTAGTCACGCGCGCCTTCCGGCGTGGCTGTGGTCAGCATCGGAGTTTCGATGTCGAGGA               >  SRR19973065.2233329/1‑150 (MQ=60)
                                                                                                                                    AAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCACCAGGTAGTCACGCGCGCCTTCCGGCGTGGCTTTGGTCAGCATCGGAGTTTCGATGTCGAGGAA              >  SRR19973065.2059894/1‑150 (MQ=60)
                                                                                                                                     AAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCACCAGGTAGTCACGCGCGCCTTCCGGCGTGGCTTTGGTCAGCATCGGAGTTTCGATGTCGAGGAAG             >  SRR19973065.1054509/1‑150 (MQ=60)
                                                                                                                                     AAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCACCAGGTAGTCACGCGCGCCTTCCGGCGTGGCTTTGGTCAGCATCGGAGTTTAGATGTCGAGGAAG             <  SRR19973065.3352123/150‑1 (MQ=60)
                                                                                                                                       ACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCACCAGGTAGTCACGCGCGCCTTCCGGCGTGGCTTTGGTCAGCATCGGAGTTTCGATGTCGAGGAAGCC           >  SRR19973065.892313/1‑150 (MQ=60)
                                                                                                                                          GGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCACCAGGTAGTCACGCGCGCCTTCCGGCGTGGCTTTGGTCAGCATCGGAGTTTCGATGTCGAGGAAGCCGTG        >  SRR19973065.2703843/1‑150 (MQ=60)
                                                                                                                                          GGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCACCAGGTAGTCACGCGCGCCTTCCGGCGTGGCTTTGGTCAGCATCGGAGTTTCGATGTCGAGGAAGCCGTG        >  SRR19973065.2419170/1‑150 (MQ=60)
                                                                                                                                             CATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCACCAGGTAGTCACGCGCGCCTTCCGGCGTGGCTTTGGTCAGCATCGGAGTTTCGATGTCGAGGAAGCCGTGGTC     >  SRR19973065.2751793/1‑150 (MQ=60)
                                                                                                                                               TCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCACCAGGTAGTCACGCGCGCCTTCCGGCGTGGCTTTGGTCAGCATCGGAGTTTCGATGTCGAGGAAGCCGTGGTCAT   <  SRR19973065.487797/150‑1 (MQ=60)
                                                                                                                                                CATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCACCAGGTAGTCACGCGCGCCTTCCGGCGTGGCTTTGGTCAGCATCGGAGTTTCGATGTCGAGGAAGCCGTGGTCATC  >  SRR19973065.1012171/1‑150 (MQ=60)
                                                                                                                                                   |                                                                                                                                                  
CGCTTCCATCACTTCACGCACTTGCGGCGCGGTCATGAAAGAAGTTTCCACATCGATCTGAGTAAATTCAGGCTGACGGTCAGCACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATCAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCACCAGGTAGTCACGCGCGCCTTCCGGCGTGGCTTTGGTCAGCATCGGAGTTTCGATGTCGAGGAAGCCGTGGTCATC  >  CP009273/1944030‑1944323

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑