Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F434 I0 R1 1130 215.9 7031662 97.1% 6827743 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,002,354 C→A 100% L199L (CTC→CTA)  yedE → UPF0394 family sulfur transport domain‑containing inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,002,3540CA99.3% 472.8 / ‑4.1 170L199L (CTC→CTA) yedEUPF0394 family sulfur transport domain‑containing inner membrane protein
Reads supporting (aligned to +/- strand):  ref base C (2/1);  new base A (77/87);  total (81/89)
Fisher's exact test for biased strand distribution p-value = 6.03e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 172 total aligned reads displayed.

TGCCATCGCCACTGCCATTGGTTCATGGTTTGGCGCGCGCTTTACCCTGCTGCCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTCGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCTGGCAATGCTGTTTGGCGTCGGCTTTGGTTTACTGATTGAACGTGCGCAAATCTGCTTTACTTCAGCGTTCCGC  >  CP009273/2002210‑2002498
                                                                                                                                                |                                                                                                                                                
tGCCATCGCCACTGCCATTGGTTCATGGTTTGGCGCGCGCTTTACCCTGCTGCCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAggg                                                                                                                                               <  1:301834/148‑1 (MQ=255)
tGCCATCGCCACTGCCATTGGTTCATGGTTTGGCGCGCGCTTTACCCTGCTGCCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAggg                                                                                                                                               <  1:326591/148‑1 (MQ=255)
tGCCATCGCCACTGCCATTGGTTCATGGTTTGGCGCGCGCTTTACCCTGCTGCCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAggg                                                                                                                                               <  1:301788/148‑1 (MQ=255)
tGCCATCGCCACTGCCATTGGTTCATGGTTTGGCGCGCGCTTTACCCTGCTGCCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAggg                                                                                                                                               <  1:1280093/148‑1 (MQ=255)
   cATCGCCACTGCCATTGGTTCATGGTTTGGCGCGCGCTTTACCCTGCTGCCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGCTg                                                                                                                                            >  2:1614393/1‑148 (MQ=255)
     tCGCCACTGCCATTGGTTCATGGTTTGGCGCGCGCTTTACCCTGCTGCCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTGTTCGTCGAGGGAGGCt                                                                                                                                          >  2:2682279/1‑148 (MQ=255)
      cGCCACTGCCATTGGTTCATGGTTTGGCGCGCGCTTTACCCTGCTGCCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTg                                                                                                                                         >  1:247008/1‑148 (MQ=255)
          aCTGCCATTGGTTCATGGTTTGGCGCGCGCTTTACCCTGCTGCCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCt                                                                                                                                     <  2:625927/148‑1 (MQ=255)
          aCTGCCATTGGTTCATGGTTTGGCGCGCGCTTTACCCTGCTGCCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTGTTCGTCTAGGGATGCTGGTCt                                                                                                                                     >  2:1506665/1‑148 (MQ=255)
           cTGCCATTGGTTCATGGTTTGGCGCGCGCTTTACCCTGCTGCCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCtt                                                                                                                                    >  2:3480422/1‑148 (MQ=255)
            tGCCATTGGTTCATGGTTTGGCGCGCGCTTTACCCTGCTGCCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCttt                                                                                                                                   <  2:3465383/148‑1 (MQ=255)
            tGCCATTGGTTCATGGTTTGGCGCGCGCTTTACCCTGCTGCCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCttt                                                                                                                                   <  2:2965727/148‑1 (MQ=255)
            tGCCATTGGTTCATGGTTTGGCGCGCGCTTTACCCTGCTGCCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCttt                                                                                                                                   <  2:3465337/148‑1 (MQ=255)
                aTTGGTTCATGGTTTGGCGCGCGCTTTACCCTGCTGCCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCg                                                                                                                               >  2:2566667/1‑148 (MQ=255)
                aTTGGTTCATGGTTTGGCGCGCGCTTTACCCTGCTGCCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTGTTCGTCTAGGGATGCTGGTCTTTTTCg                                                                                                                               >  2:2566153/1‑148 (MQ=255)
                 ttGGTTCATGGTTTGGCGCGCGCTTTACCCTGCTGCCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCgg                                                                                                                              >  2:176297/1‑148 (MQ=255)
                 ttGGTTCATGGTTTGGCGCGCGCTTTACCCTGCTGCCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTGTTGTGTAGGGGTGCTGGTGTTTTTCgg                                                                                                                              >  1:2654687/1‑148 (MQ=255)
                  tGGTTCATGGTTTGGCGCGCGCTTTACCCTGCTGCCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGc                                                                                                                             <  1:3251530/148‑1 (MQ=255)
                  tGGTTCATGGTTTGGCGCGCGCTTTACCCTGCTGCCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGc                                                                                                                             >  2:2392694/1‑148 (MQ=255)
                  tGGTTCATGGTTTGGCGCGCGCTTTACCCTGCTGCCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGc                                                                                                                             >  2:2146497/1‑148 (MQ=255)
                  tGGTTCATGGTTTGGCGCGCGCTTTACCCTGCTGCCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGc                                                                                                                             <  1:481587/148‑1 (MQ=255)
                   ggTTCATGGTTTGGCGCGCGCTTTACCCTGCTGCCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCa                                                                                                                            <  2:2230614/148‑1 (MQ=255)
                   ggTTCATGGTTTGGCGCGCGCTTTACCCTGCTGCCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCa                                                                                                                            <  1:3302044/148‑1 (MQ=255)
                   ggTTCATGGTTTGGCGCGCGCTTTACCCTGCTGCCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCa                                                                                                                            <  1:3302285/148‑1 (MQ=255)
                     ttCATGGTTTGGCGCGCGCTTTACCCTGCTGCCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATg                                                                                                                          >  2:703641/1‑148 (MQ=255)
                     ttCATGGTTTGGCGCGCGCTTTACCCTGCTGCCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATg                                                                                                                          <  2:769394/148‑1 (MQ=255)
                          ggTTTGGCGCGCGCTTTACCCTGCTGCCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTGTTCGACTAGGGGTGCTGGTCTTTTTAGGCATgcgggg                                                                                                                     >  1:1699378/1‑148 (MQ=255)
                          ggTTTGGCGCGCGCTTCACCCTGCTGCCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTAGGCATgctggg                                                                                                                     >  1:238473/1‑148 (MQ=255)
                                 cgcgcgcTTTACCCTGCTGCCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGgcg                                                                                                              <  2:901822/148‑1 (MQ=255)
                                 cgcgcgcTTTACCCTGCTGCCCATCTTCCGTAGTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGgcg                                                                                                              <  2:1502907/148‑1 (MQ=255)
                                   cgcgcTTTACCCTGCTGCCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCgct                                                                                                            >  2:2712444/1‑148 (MQ=255)
                                      gcTTTACCCTGCTGCCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCgctgct                                                                                                         <  2:2510250/148‑1 (MQ=255)
                                      gcTTTACCCTGCTGCCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCGGGGCgctgct                                                                                                         >  2:841165/1‑148 (MQ=255)
                                        tttACCCTGCTGCCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTca                                                                                                       <  1:1379413/148‑1 (MQ=255)
                                        tttACCCTGCTGCCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTGTTCGTCTAGGGGTTCTGGTCTTTTTCGGCCAGCTGGGCTGGGCGCTGCTca                                                                                                       >  1:2339738/1‑148 (MQ=255)
                                         ttACCCTGCTGCCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTGGTCTAGGGATGGGGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTcac                                                                                                      >  1:1129311/1‑148 (MQ=255)
                                         ttACCCTGCTGCCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGGTGGTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTcac                                                                                                      >  1:1117008/1‑148 (MQ=255)
                                           aCCCTGCTGCCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAg                                                                                                    <  2:2666307/148‑1 (MQ=255)
                                              ctgctgCCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGa                                                                                                 <  2:969935/148‑1 (MQ=255)
                                                   gcCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTGTAGGGATGCTGGTCTTTTTCGGCCTGCTGGGCTGGGCGCTGCTCACAGCGATGaac                                                                                            >  1:2845910/1‑148 (MQ=255)
                                                     ccATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCGGGTATTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGAAGaacca                                                                                          >  1:3078316/1‑148 (MQ=255)
                                                       aTCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGaaccaac                                                                                        <  2:2916479/148‑1 (MQ=255)
                                                           tCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTGTTGGTCATGGGATGCGGGTGTTTTTCGGCCTGATGGGGGGGGCGCTGCGCACAGCGAGGAACCAACCCac                                                                                    >  1:1674051/1‑147 (MQ=255)
                                                                aTTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTgg                                                                               >  2:1942911/1‑148 (MQ=255)
                                                                aTTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCGGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTgg                                                                               >  2:157500/1‑148 (MQ=255)
                                                                 ttCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTggg                                                                              <  1:1170205/148‑1 (MQ=255)
                                                                 ttCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTggg                                                                              <  1:951952/148‑1 (MQ=255)
                                                                 ttCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTggg                                                                              >  2:1733630/1‑148 (MQ=255)
                                                                   cccGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTGTTGGTGTAGGGATGCTGGTCTTTTTGGGCATGCGGGGGTGGGCGCTGCTCACAGCGATGAACCAACCCCAACAGGGGc                                                                            >  1:1292858/1‑148 (MQ=255)
                                                                    ccGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCt                                                                           <  1:3200514/148‑1 (MQ=255)
                                                                    ccGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTGTTCGTCGAGGGCTGCTGGTGTTTTTCGGCATGCTGGGGTGGGCGCTGCTCACAGCGATGAACCAACCCAAACAGGGGcg                                                                           >  1:532591/1‑147 (MQ=255)
                                                                      gTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTGTTGGTCTCGGGCGGCGGGTCTTTTTCGGCCTGGTGGGCGGGGCGCTGCTCACAGCGATGAACCAACCCCAACTGGGGCTgg                                                                         >  2:2952721/1‑148 (MQ=255)
                                                                           aaTGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCTGGCAATg                                                                    <  2:647433/148‑1 (MQ=255)
                                                                           aaTGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCTGGCAATg                                                                    <  2:3361768/148‑1 (MQ=255)
                                                                             tGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCTGGCAATGCt                                                                  >  2:2872208/1‑148 (MQ=255)
                                                                             tGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCTGGCAATGCt                                                                  <  1:2566667/148‑1 (MQ=255)
                                                                             tGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCTGGCAATGCt                                                                  <  1:2566153/148‑1 (MQ=255)
                                                                             tGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTGTTGGTGTTGGGGTGGGGGTGTTTTTTGGGATTCGGGGGGGGGCGGGGCGCACACCCATGTAACAAACCAAAAAGGGGGGGGCCATGcg                                                                  >  1:893351/1‑147 (MQ=255)
                                                                              gCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGGCTAGGGGTGGTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCTGGGCATGCTg                                                                 >  1:2264437/1‑148 (MQ=255)
                                                                                 gAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATTCGGGTGTTTTTCGGCGTGGTGGGCTGGGCGCAGCTCAAAGCGATGAACCAACCCCAACGGGGGCGGGCACAGCGGttt                                                              >  2:2107576/1‑148 (MQ=255)
                                                                                   aaGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCGGGCAATGCTGTTTgg                                                            >  2:2780838/1‑148 (MQ=255)
                                                                                     gTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTTGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCTGGCAATGCTGTTTGGCg                                                          <  1:2107576/148‑1 (MQ=255)
                                                                                      tttCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCTGGCAATGCTGTTTGGCGt                                                         <  2:2119678/148‑1 (MQ=255)
                                                                                      tttCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCTGGCAATGCTGTTTGGCGt                                                         <  2:1239106/148‑1 (MQ=255)
                                                                                      tttCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCGGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCGGGCAATGCGGGTTGGCGt                                                         >  2:44709/1‑148 (MQ=255)
                                                                                       ttCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCCTGCGGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCTGGCAATGCTGTTTGGCGTc                                                        >  2:163029/1‑148 (MQ=255)
                                                                                          tGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCTGGCAATGCTGTTTGGCGTCGGc                                                     <  2:431602/148‑1 (MQ=255)
                                                                                          tGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCTGGCAATGCTGTTTGGCGTCGGc                                                     <  2:3318358/148‑1 (MQ=255)
                                                                                          tGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCTGGCAATGCTGTTTGGCGTCGGc                                                     <  2:1766795/148‑1 (MQ=255)
                                                                                          tGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCTGGCAATGCTGTTTGGCGTCGGc                                                     <  1:1143127/148‑1 (MQ=255)
                                                                                            ccgccTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCTGGCAATGCTGTTTGGCGTCGGCtt                                                   <  1:1768347/148‑1 (MQ=255)
                                                                                                 tCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCTGGCAATGCTGTTTGGCGTCGGCTTTGGtt                                              <  2:2505807/148‑1 (MQ=255)
                                                                                                 tCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCTGGCAATGCTGTTTGGCGTCGGCTTTGGtt                                              <  2:2505731/148‑1 (MQ=255)
                                                                                                 tCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCTGGCAATGCTGTTTGGCGTCGGCTTTGGtt                                              <  2:2473381/148‑1 (MQ=255)
                                                                                                    ccGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTGTCGTGTAGGGATGCTGGTGTTTTTCGGCCTTCGGGGCTGGGCGCTGCTCACAGCGAGGAACCAACCCAAACTGGGGCTGGCAATGCGGTTTGGCGGCGGCTTTGGTTTAc                                           >  1:1392381/1‑148 (MQ=255)
                                                                                                       cTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCTGGCAATGCTGTTTGGCGTCGGCTTTGGTTTACTGa                                        <  1:1974222/148‑1 (MQ=255)
                                                                                                        tGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTCGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCTGGCAATGCTGTTTGGCGTCGGCTTTGGTTTACTGAt                                       <  2:2481029/148‑1 (MQ=255)
                                                                                                          aCGCAAAAACCGGATCAGGCGCGGCGTCGTTTTGGTGTAGGGATGGGGGTCTTTTTGGGCATGCGGGGGTGGGCGCTGCTCACAGCGATGAACCAACCCAAAAAGGGGCGGGCCAAGCTGTTTGGCGTCGGGGGTGGTTTAATTATTg                                     >  1:3087216/1‑148 (MQ=255)
                                                                                                          aCGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCGGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCTGGCAATGCTGTTTGGCGTCGGCGTTGGTTTACTGATTg                                     >  2:3137211/1‑148 (MQ=255)
                                                                                                          aCGCAAAAACCGGATCAGGCGCGGCGTCGTGTTGGTCTAGGGCTGGTGGTCTTTTTGGGGATGCTGGGGGGGGCGCTGCTCACAGCGCTGAAACAACCCCAACAGGGGCGGGCAATGCTGTTTGGCGTCGGGGTTGGTTTAATGtttt                                     >  1:3087172/1‑147 (MQ=255)
                                                                                                                aaaCCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCTGGCAATGCTGTTTGGCGTCGGCTTTGGTTTACTGATTGAACGTg                               <  1:3256322/148‑1 (MQ=255)
                                                                                                                aaaCCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCTGGCAATGCTGTTTGGCGTCGGCTTTGGTTTACTGATTGAACGTg                               >  2:274777/1‑148 (MQ=255)
                                                                                                                 aaCCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCTGGCAATGCTGTTTGGCGTCGGCTTTGGTTTACTGATTGAACGTgc                              <  1:3141924/148‑1 (MQ=255)
                                                                                                                     ggATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCTGGCAATGCTGTTTGGCGTCGGCTTTGGTTTACTGATTGAACGTGCGCaa                          <  2:3207806/148‑1 (MQ=255)
                                                                                                                     ggATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCTGGCAATGCTGTTTGGCGTCGGCTTTGGTTTACTGATTGAACGTGCGCaa                          <  1:2146497/148‑1 (MQ=255)
                                                                                                                     ggATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCTGGCAATGCTGTTTGGCGTCGGCTTTGGTTTACTGATTGAACGTGCGCaa                          <  2:867728/148‑1 (MQ=255)
                                                                                                                          aGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCTGGCAATGCTGTTTGGCGTCGGCTTTGGTTTACTGATTGAACGTGCGCAAATCTg                     <  2:2408271/148‑1 (MQ=255)
                                                                                                                                     cgtTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCTGGCAATGCTGTTTGGCGTCGGCTTTGGTTTACTGATTGAACGTGCGCAAATCTGCTTTACTTCAg          <  2:474168/148‑1 (MQ=255)
                                                                                                                                     cgtTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCTGGCAATGCTGTTTGGCGTCGGCTTTGGTTTACTGATTGAACGTGCGCAAATCTGCTTTACTTCAg          <  2:1343169/148‑1 (MQ=255)
                                                                                                                                     cgtTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCTGGCAATGCTGTTTGGCGTCGGCTTTGGTTTACTGATTGAACGTGCGCAAATCTGCTTTAATTCAg          >  2:1572944/1‑148 (MQ=255)
                                                                                                                                       ttttCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCTGGCAATGCTGTTTGGCGTCGGCTTTGGTTTACTGATTGAACGTGCGCAAATCTGCTTTACTTCAGCg        >  2:3494132/1‑148 (MQ=255)
                                                                                                                                       ttttCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCTGGCAATGCTGTTTGGCGTCGGCTTTGGTTTACTGATTGAACGTGCGCAAATCTGCTTTACTTCAGCg        <  1:1614393/148‑1 (MQ=255)
                                                                                                                                         ttCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCTGGCAATGCTGTTTGGCGTCGGCTTTGGTTTACTGATTGAACGTGCGCAAATCTGCTTTACTTCAGCGtt      <  2:61687/148‑1 (MQ=255)
                                                                                                                                          tcttctaGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCTGGCAATGCTGTTTGGCGTCGGCTTTGGTTTACTGATTGAACGTGCGCAAATCTGCTTTACTTCAGCGTTc     <  2:2627403/145‑1 (MQ=255)
                                                                                                                                          tCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCTGGCAATGCTGTTTGGCGTCGGCTTTGGTTTACTGATTGAACGTGCGCAAATCTGCTTTACTTCAGCGTTc     <  1:727963/148‑1 (MQ=255)
                                                                                                                                          tCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCTGGCAATGCTGTTTGGCGTCGGCTTTGGTTTACTGATTGAACGTGCGCAAATCTGCTTTACTTCAGCGTTc     >  2:1030718/1‑148 (MQ=255)
                                                                                                                                          tCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCTGGCAATGCTGTTTGGCGTCGGCTTTGGTTTACTGATTGAACGTGCGCAAATCTGCTTTACTTCAGCGTTc     <  1:1007735/148‑1 (MQ=255)
                                                                                                                                          tCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCTGGCAATGCTGTTTGGCGTCGGCTTTGGTTTACTGATTGAACGTGCGCAAATCTGCTTTACTTCAGCGTTc     >  2:1030976/1‑148 (MQ=255)
                                                                                                                                           cGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCTGGCAATGCTGTTTGGCGTCGGCTTTGGTTTACTGATTGAACGTGCGCAAATCTGCTTTACTTCAGCGTTcc    >  1:2484967/1‑148 (MQ=255)
                                                                                                                                            gTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCTGGCAATGCTGTTTGGCGTCGGCTTTGGTTTACTGATTGAACGTGCGCAAATCTGCTTTACTTCAGCGTTCcg   <  2:2956285/148‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                                
TGCCATCGCCACTGCCATTGGTTCATGGTTTGGCGCGCGCTTTACCCTGCTGCCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTCGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCTGGCAATGCTGTTTGGCGTCGGCTTTGGTTTACTGATTGAACGTGCGCAAATCTGCTTTACTTCAGCGTTCCGC  >  CP009273/2002210‑2002498

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TGCCATCGCCACTGCCATTGGTTCATGGTTTGGCGCGCGCTTTACCCTGCTGCCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTCGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCTGGCAATGCTGTTTGGCGTCGGCTTTGGTTTACTGATTGAACGTGCGCAAATCTGCTTTACTTCAGCGTTCCGCGATAT  >  CP009273/2002210‑2002503
                                                                                                                                                |                                                                                                                                                     
TGCCATCGCCACTGCCATTGGTTCATGGTTTGGCGCGCGCTTTACCCTGCTGCCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGAT                                                                                                                                                  <  SRR19973065.303759/150‑1 (MQ=60)
TGCCATCGCCACTGCCATTGGTTCATGGTTTGGCGCGCGCTTTACCCTGCTGCCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGAT                                                                                                                                                  <  SRR19973065.303805/150‑1 (MQ=60)
TGCCATCGCCACTGCCATTGGTTCATGGTTTGGCGCGCGCTTTACCCTGCTGCCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGAT                                                                                                                                                  <  SRR19973065.1288440/150‑1 (MQ=60)
TGCCATCGCCACTGCCATTGGTTCATGGTTTGGCGCGCGCTTTACCCTGCTGCCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGAT                                                                                                                                                  <  SRR19973065.1288544/150‑1 (MQ=60)
TGCCATCGCCACTGCCATTGGTTCATGGTTTGGCGCGCGCTTTACCCTGCTGCCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGAT                                                                                                                                                  <  SRR19973065.328864/150‑1 (MQ=60)
TGCCATCGCCACTGCCATTGGTTCATGGTTTGGCGCGCGCTTTACCCTGCTGCCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGAT                                                                                                                                                  <  SRR19973065.328911/150‑1 (MQ=60)
    ATCGCCACTGCCATTGGTTCATGGTTTGGCGCGCGCTTTACCCTGCTGCCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTG                                                                                                                                              <  SRR19973065.2611652/150‑1 (MQ=60)
    ATCGCCACTGCCATTGGTTCATGGTTTGGCGCGCGCTTTACCCTGCTGCCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTG                                                                                                                                              >  SRR19973065.248754/1‑150 (MQ=60)
        CCACTGCCATTGGTTCATGGTTTGGCGCGCGCTTTACCCTGCTGCCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTGTTCGTCTAGGGATGCTGGTCT                                                                                                                                          >  SRR19973065.2246259/1‑150 (MQ=60)
              CCATTGGTTAATGGTTTGGCGCGCGCTTTACCCTGCTGCCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCG                                                                                                                                    <  SRR19973065.1565232/150‑1 (MQ=60)
               CATTGGTTCATGGTTTGGCGCGCGCTTTACCCTGCTGCCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTGTTGTGTAGGGGTGCTGGTGTTTTTCGG                                                                                                                                   >  SRR19973065.2671088/1‑150 (MQ=60)
                  TTGTTCATGGTTTGGCGCGCGCTTTACCCTGCTGCCCATCTTCCGTAGTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCAT                                                                                                                                <  SRR19973065.3271183/150‑1 (MQ=60)
                  TGGTTCATGGTTTGGCGCGCGCTTTACCCTGCTGCCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCAT                                                                                                                                <  SRR19973065.3271185/150‑1 (MQ=60)
                  TGGTTCATGGTTTGGCGCGCGCTTTACCCTGCTGCCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCAT                                                                                                                                <  SRR19973065.484864/150‑1 (MQ=60)
                   GGTTCATGGTTTGGCGCGCGCTTTACCCTGCTGCCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATG                                                                                                                               <  SRR19973065.3322153/150‑1 (MQ=60)
                   GGTTCATGGTTTGGCGCGCGCTTTACCCTGCTGCCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATG                                                                                                                               <  SRR19973065.3322395/150‑1 (MQ=60)
                        ATGGTTTGGCGCGCGCTTTACCCTGCTGCCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTGGTCTCGGGCTGCTGGTCTTTTTCGGCATGCTGGG                                                                                                                          >  SRR19973065.2496648/1‑150 (MQ=60)
                        ATGGTTTGGCGCGCGCTTTACCCTGCTGCCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTGTTCGTCTAGGGATGCTGGTCTTTTTAGGCATGCTGGG                                                                                                                          >  SRR19973065.1883188/1‑150 (MQ=60)
                        ATGGTTTGGCGCGCGCTTTACCCTGCTGCCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTGTTCGACTAGGGGTGCTGGTCTTTTTAGGCATGCGGGG                                                                                                                          >  SRR19973065.1710709/1‑150 (MQ=60)
                        ATGGTTTGGCGCGCGCTTCACCCTGCTGCCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTAGGCATGCTGGG                                                                                                                          >  SRR19973065.240183/1‑150 (MQ=60)
                           GTTTGGCGCGCGCTTTACCCTGCTGCCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTG                                                                                                                       <  SRR19973065.3115126/150‑1 (MQ=60)
                                  GCGCGCTTTACCCTGCTGCCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTG                                                                                                                <  SRR19973065.491755/150‑1 (MQ=60)
                                      GCTTTACCCTGCTGCCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTGTTCGTCTAGGGGTTCTGGTCTTTTTCGGCCAGCTGGGCTGGGCGCTGCTCA                                                                                                            >  SRR19973065.2354345/1‑150 (MQ=60)
                                       CTTTACCCTGCTGCCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTGGTCTAGGGATGGGGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCAC                                                                                                           >  SRR19973065.1136651/1‑150 (MQ=60)
                                       CTTTACCCTGCTGCCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGGTGGTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCAC                                                                                                           >  SRR19973065.1124300/1‑150 (MQ=60)
                                       CTTTACCCTGCTGCCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTGTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCGGGGCGCTGCTCAC                                                                                                           >  SRR19973065.1136637/1‑150 (MQ=60)
                                        TTTACCCTGCTGCCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACA                                                                                                          <  SRR19973065.1388500/150‑1 (MQ=60)
                                         TTACCCTGCTGCCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAG                                                                                                         <  SRR19973065.2280538/150‑1 (MQ=60)
                                                 CTGCCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTGTAGGGATGCTGGTCTTTTTCGGCCTGCTGGGCTGGGCGCTGCTCACAGCGATGAAC                                                                                                 >  SRR19973065.2863421/1‑150 (MQ=60)
                                                   GCCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTGGTGTAGGGCTGCTGGTGTTTTTCGGCCTGCTGGGGTGGGCGCTGCGCACAGCGATGAACCA                                                                                               >  SRR19973065.3097213/1‑150 (MQ=60)
                                                   GCCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGGTGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCA                                                                                               >  SRR19973065.3161619/1‑150 (MQ=60)
                                                   GCCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCGGGTATTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGAAGAACCA                                                                                               >  SRR19973065.3097119/1‑150 (MQ=60)
                                                   GCCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTGTTGGTGTAGGGGTGCGGGTCTTTTTCGGCATGCTGGGCGGGGGGCTGCTCACAGCGATGAACCA                                                                                               >  SRR19973065.946195/1‑150 (MQ=60)
                                                         CTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTGTTGGTCATGGGATGCGGGTGTTTTTCGGCCTGATGGGGGGGGCGCTGCGCACAGCGAGGAACCAACCCAC                                                                                         >  SRR19973065.1685248/1‑150 (MQ=60)
                                                                ATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTGTTCGTCTAGGGGTGGGGGTCTTTTTCGGCCTGCTGGGCGGGGCGCTGCTCACAGCGATGAACCAACCCCAACTGGGG                                                                                  >  SRR19973065.3057419/1‑150 (MQ=60)
                                                                 TTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGC                                                                                 <  SRR19973065.958245/150‑1 (MQ=60)
                                                                 TTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGC                                                                                 <  SRR19973065.1177663/150‑1 (MQ=60)
                                                                 TTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTGTTGGTGTAGGGATGCTGGTCTTTTTGGGCATGCGGGGGTGGGCGCTGCTCACAGCGATGAACCAACCCCAACAGGGGC                                                                                 >  SRR19973065.1301377/1‑150 (MQ=60)
                                                                  TCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTGTTCGTCGAGGGCTGCTGGTGTTTTTCGGCATGCTGGGGTGGGCGCTGCTCACAGCGATGAACCAACCCAAACAGGGGCG                                                                                >  SRR19973065.536069/1‑150 (MQ=60)
                                                                   CCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTGTTGGGGTAGGGATGCTGGTCTTTTTGGGCCGGCGGGGCTGGGCGCGGCTCACAGCGCTGAACCAACCCccacaggggcgg                                                                               >  SRR19973065.1293332/1‑138 (MQ=60)
                                                                    CCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCTGG                                                                              <  SRR19973065.3219787/150‑1 (MQ=60)
                                                                      GTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTGGTGTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCGGGGCGCTGCTCACAGCGCTGAACCAACCCAAACTGGGGCTGGCA                                                                            >  SRR19973065.2034193/1‑150 (MQ=60)
                                                                        GAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCTGGCAAT                                                                          <  SRR19973065.3031631/150‑1 (MQ=60)
                                                                           AATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCTGGCAATGCT                                                                       <  SRR19973065.1724863/150‑1 (MQ=60)
                                                                            ATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGGCTAGGGGTGGTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCTGGGCATGCTG                                                                      >  SRR19973065.2278752/1‑150 (MQ=60)
                                                                             TGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCTGGCAATGCTGT                                                                     <  SRR19973065.2582180/150‑1 (MQ=60)
                                                                             TGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCTGGCAATGCTGT                                                                     <  SRR19973065.2582696/150‑1 (MQ=60)
                                                                                  AAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTGTTGGTGTAGGGGTGGTGGTGTTTTTGGGCATGCTGGGCGGGGCGCTGCTCACAGCGCTGAAACAACCCAAACAGGGGCTGGgcatgggggtttgg                                                                >  SRR19973065.719949/1‑136 (MQ=60)
                                                                                     GTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTTGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCTGGCAATGCTGTTTGGCGTC                                                             <  SRR19973065.2121128/150‑1 (MQ=60)
                                                                                     GTGTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCTGGCAATGCTGTTTGGCGTC                                                             <  SRR19973065.255165/150‑1 (MQ=60)
                                                                                          TGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCTGGCAATGCTGTTTGGCGTCGGCTT                                                        <  SRR19973065.1150508/150‑1 (MQ=60)
                                                                                          TGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCTGGCAATGCTGTTTGGCGTCGGCTT                                                        <  SRR19973065.1150461/150‑1 (MQ=60)
                                                                                            CCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCTGGCAATGCTGTTTGGCGTCGGCTTTG                                                      <  SRR19973065.1779936/150‑1 (MQ=60)
                                                                                                  CACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTGTCGTGTAGGGATGCTGGTGTTTTTCGGCCTTCGGGGCTGGGCGCTGCTCACAGCGAGGAACCAACCCAAACTGGGGCTGGCAATGCGGTTTGGCGGCGGCTTTGGTTTAC                                                >  SRR19973065.1401521/1‑150 (MQ=60)
                                                                                                       CTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCTGGCAATGCTGTTTGGCGTCGGCTTTGGTTTACTGATT                                           <  SRR19973065.1987076/150‑1 (MQ=60)
                                                                                                        TGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTGGTGTAGGGATGGGGGTCTTTTTGGGCATGCGGGGGTGGGCGCTGCTCACAGCGATGAACCAACCCAAAAAGGGGCGGGCCAAGCTGTTTGGCGTCGGgggtggtttaattattg                                          >  SRR19973065.3106070/1‑133 (MQ=60)
                                                                                                        TGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTGGTGTAGGGATGCTGGTGTTTTTGGGCATGCGGGGCGGGGCGCTGCTGACAGCGATGAACCAACCCAAAAAGGGGCGGGCAAAGCTGTTTGGCGTCGGCGTTGGTTTAATGATTT                                          >  SRR19973065.3105977/1‑150 (MQ=60)
                                                                                                        TGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTAGTCTAGGGATGCTGGTCTTTTTCGGCCTGCTGGGCTGGGCGCTGCTCACAGCGGAGAACCAACCCCAAAAGGGGCTGGCAATGCTGTTTTGCGGCGGCTTTGGTTTACTGATTT                                          >  SRR19973065.2308583/1‑150 (MQ=60)
                                                                                                        TGACGCAAAAACCGGATCAGGCGCGGCGTCGTGTTGGTCTAGGGCTGGTGGTCTTTTTGGGGATGCTGGGGGGGGCGCTGCTCACAGCGCTGAAACAACCCCAACAGGGGCGGGCAATGCTGTTTGGCGTCGGggttggtttaatgtttt                                          >  SRR19973065.3106026/1‑133 (MQ=60)
                                                                                                             CAAAAACCGGATCAGGCGCGGCGTCGTTTTGGTGTATGGGTGGGGGTGTTTTTAGGGATGCTGGGCGGGGCGCTGGTCACACCGATGAACCAACCCAAACAGGGGCTGGCAATGGTGTTTGGCGTCGGgggttgttttatgattttaagt                                     >  SRR19973065.716695/1‑128 (MQ=48)
                                                                                                                AAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCTGGCAATGCTGTTTGGCGTCGGCTTTGGTTTACTGATTGAACGTGCG                                  <  SRR19973065.3276044/150‑1 (MQ=60)
                                                                                                                AAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCTGGCAATGCTGTTTGGCGTCGGCTTTGGTTTACTGATTGAACGTGCG                                  <  SRR19973065.3276021/150‑1 (MQ=60)
                                                                                                                 AACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCTGGCAATGCTGTTTGGCGTCGGCTTTGGTTTACTGATTGAACGTGCGC                                 <  SRR19973065.3161019/150‑1 (MQ=60)
                                                                                                                     GGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCTGGCAATGCTGTTTGGCGTCGGCTTTGGTTTACTGATTGAACGTGCGCAAAT                             <  SRR19973065.2160301/150‑1 (MQ=60)
                                                                                                                                   GTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCTGGCAATGCTGTTTGGCGTCGGCTTTGGTTTACTGATTGAACGTGCGCAAATCTGCTTTACTTCAG               <  SRR19973065.2775456/150‑1 (MQ=60)
                                                                                                                                   GTCGTTTTCGTCTAGGGATGCTGGTCTTTTTAGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACAGGGGCTGGCAATGCTGTTTGGCGTCGGCTTTGGTTTAATGATTTAAAGTGCGCAAATCatctttaattcat               >  SRR19973065.725731/1‑137 (MQ=60)
                                                                                                                                    TCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCTGGCAATGCTGTTTGGCGTCGGCTTTGGTTTACTGATTGAACGTGCGCAAATCTGCTTTACTTCAGC              <  SRR19973065.3501603/150‑1 (MQ=60)
                                                                                                                                       TTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCTGGCAATGCTGTTTGGCGTCGGCTTTGGTTTACTGATTGAACGTGCGCAAATCTGCTTTACTTCAGCGTT           <  SRR19973065.1625225/150‑1 (MQ=60)
                                                                                                                                         TTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCTGGCAATGCTGTTTGGCGTCGGCTTTGGTTTACTGATTGAACGTGCGCAAATCTGCTTTACTTCAGCGTTCC         >  SRR19973065.2500603/1‑150 (MQ=60)
                                                                                                                                          TCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCTGGCAATGCTGTTTGGCGTCGGCTTTGGTTTACTGATTGAACGTGCGCAAATCTGCTTTACTTCAGCGTTCCG        <  SRR19973065.1014421/150‑1 (MQ=60)
                                                                                                                                          TCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCTGGCAATGCTGTTTGGCGTCGGCTTTGGTTTACTGATTGAACGTGCGCAAATCTGCTTTACTTCAGCGTTCCG        <  SRR19973065.732838/150‑1 (MQ=60)
                                                                                                                                           CGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCTGGCAATGCTGTTTGGCGTCGGCTTTGGTTTACTGATTGAACGTGCGCAAATCTGCTTTACTTCAGCGTTCCGC       >  SRR19973065.1635935/1‑150 (MQ=60)
                                                                                                                                            GTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCTGGCAATGCTGTTTGGCGTCGGCTTTGGTTTACTGATTGAACGTGCGCAAATCTGCTTTACTTCAGCGTTCCGCG      >  SRR19973065.2760543/1‑150 (MQ=60)
                                                                                                                                              CTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCTGGCAATGCTGTTTGGCGTCGGCTTTGGTTTACTGATTGAACGTGCGCAAATCTGCTTTACTTCAGCGTTCCGCGAT    <  SRR19973065.2604895/150‑1 (MQ=60)
                                                                                                                                               TAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCTGGCAATGCTGTTTGGCGTCGGCTTTGGTTTACTGATTGAACGTGCGCAAATCTGCTTTACTTCAGCGTTCCGCGATA   >  SRR19973065.2146382/1‑150 (MQ=60)
                                                                                                                                               TAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCTGGCAATGCTGTTTGGCGTCGGCTTTGGTTTACTGATTGAACGTGCGCAAATCTGCTTTACTTCAGCGTTCCGCGATA   >  SRR19973065.3092547/1‑150 (MQ=60)
                                                                                                                                                AGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCTGGCAATGCTGTTTGGCGTCGGCTTTGGTTTACTGATTGAACGTGCGCAAATCTGCTTTACTTCAGCGTTCCGCGATAT  >  SRR19973065.3315062/1‑150 (MQ=60)
                                                                                                                                                AGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCTGGCAATGCTGTTTGGCGTCGGCTTTGGTTTACTGATTGAACGTGCGCAAATCTGCTTTACTTCAGCGTTCCGCGATAT  >  SRR19973065.3315047/1‑150 (MQ=60)
                                                                                                                                                AGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCTGGCAATGCTGTTTGGCGTCGGCTTTGGTTTACTGATTGAACGTGCGCAAATCTGCTTTACTTCAGCGTTCCGCGATAT  >  SRR19973065.2136843/1‑150 (MQ=60)
                                                                                                                                                |                                                                                                                                                     
TGCCATCGCCACTGCCATTGGTTCATGGTTTGGCGCGCGCTTTACCCTGCTGCCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTCGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCTGGCAATGCTGTTTGGCGTCGGCTTTGGTTTACTGATTGAACGTGCGCAAATCTGCTTTACTTCAGCGTTCCGCGATAT  >  CP009273/2002210‑2002503

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑