Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F434 I0 R1 1130 215.9 7031662 97.1% 6827743 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,138,039 C→A 100% I431I (ATC→ATA)  yegE → putative diguanylate cyclase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,138,0390CA99.1% 693.2 / ‑3.2 216I431I (ATC→ATA) yegEputative diguanylate cyclase
Reads supporting (aligned to +/- strand):  ref base C (1/1);  new base A (107/106);  total (108/108)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 216 total aligned reads displayed.

GCAATGGCGATGTTGTCTGGGCGTTGCTTGCCGTCTCACTGGTGCGCCACACGGATGGCACGCCGCTCTATTTTATCGCTCAGATTGAAGACATTAACGAGCTAAAACGCACCGAACAGGTCAATCAGCAACTGATGGAGCGCATCACTCTGGCTAACGAAGCGGGCGGGATTGGCATCTGGGAGTGGGAGTTGAAGCCGAATATTTTTAGCTGGGATAAGCGGATGTTCGAGCTGTATGAAATTCCTCCACATATCAAACCGAACTGGCAGGTGTGGTACGAGTGCGTG  >  CP009273/2137894‑2138183
                                                                                                                                                 |                                                                                                                                                
gCAATGGCGATGTTGTCTGGGCGTTGCTTGCCGTCTCACTGGTGCGCCACACGGATGGCACGCCGCTCTATTTTATCGCTCAGATTGAAGACATTAACGAGCTAAAACGCACCGAACAGGTCAATCAGCAACTGATGGAGCGCATAAc                                                                                                                                                >  2:3296302/1‑148 (MQ=255)
 cAATGGCGATGTTGTCTGGGCGTTGCTTGCCGTCTCACTGGTGCGCCACACGGATGGCACGCCGCTCTATTTTATCGCTCAGATTGAAGACATTAACGAGCTAAAACGCACCGAACAGGTCAATCAGCAACTGATGGAGCGCATAAct                                                                                                                                               >  2:2231786/1‑148 (MQ=255)
  aaTGGCGATGTTGTCTGGGCGTTGCTTGCCGTCTCACTGGTGCGCCACACGGATGGCACGCCGCTCTATTTTATCGCTCAGATTGAAGACATTAACGAGCTAAAACGCACCGAACAGGTCAATCAGCAACTGATGGAGCGCATAActc                                                                                                                                              >  2:1468216/1‑148 (MQ=255)
   aTGGCGATGTTGTCTGGGCGTTGCTTGCCGTCTCACTGGTGCGCCACACGGATGGCACGCCGCTCTATTTTATCGCTCAGATTGAAGACATTAACGAGCTAAAACGCACCGAACAGGTCAATCAGCAACTGATGGAGCGCATAActct                                                                                                                                             >  1:1118041/1‑148 (MQ=255)
     ggCGATGTTGTCTGGGCGTTGCTTGCCGTCTCACTGGTGCGCCACACGGATGGCACGCCGCTCTATTTTATCGCTCAGATTGAAGACATTAACGAGCTAAAACGCACCGAACAGGTCAATCAGCAACTGATGGAGCGCATAACTCTgg                                                                                                                                           <  1:404871/148‑1 (MQ=255)
     ggCGATGTTGTCTGGGCGTTGCTTGCCGTCTCACTGGTGCGCCACACGGATGGCACGCCGCTCTATTTTATCGCTCAGATTGAAGACATTAACGAGCTAAAACGCACCGAACAGGTCAATCAGCAACTGATGGAGCGCATAACTCTgg                                                                                                                                           >  1:2325228/1‑148 (MQ=255)
      gCGATGTTGTCTGGGCGTTGCTTGCCGTCTCACTGGTGCGCCACACGGATGGCACGCCGCTCTATTTTATCGCTCAGATTGAAGACATTAACGAGCTAAAACGCACCGAACAGGTCAATCAGCAACTGATGGAGCGCATAACTCTGGc                                                                                                                                          <  1:3467039/148‑1 (MQ=255)
         aTGTTGTCTGGGCGTTGCTTGCCGTCTCACTGGTGCGCCACACGGATGGCACGCCGCTCTATTTTATCGCTCAGATTGAAGACATTAACGAGCTAAAACGCACCGAACAGGTCAATCAGCAACTGATGGAGCGCATAACTCTGGCTaa                                                                                                                                       <  1:2117303/148‑1 (MQ=255)
            ttgtCTGGGCGTTGCTTGCCGTCTCACTGGTGCGCCACACGGATGGCACGCCGCTCTATTTTATCGCTCAGATTGAAGACATTAACGAGCTAAAACGCACCGAACAGGTCAATCAGCAACTGATGGAGCGCATAACTCTGGCTAACGa                                                                                                                                    >  2:376918/1‑148 (MQ=255)
                       ttgcttgcCGTCTCACTGGTGCGCCACACGGATGGCACGCCGCTCTATTTTATCGCTCAGATTGAAGACATTAACGAGCTAAAACGCACCGAACAGGTCAATCAGCAACTGATGGAGCGCATAACTCTGGCTAACGAAGCGGGCGGGa                                                                                                                         >  2:2936400/1‑148 (MQ=255)
                               cGTCTCACTGGTGCGCCACACGGATGGCACGCCGCTCTATTTTATCGCTCAGATTGAAGACATTAACGAGCTAAAACGCACCGAACAGGTCAATCAGCAACTGATGGAGCGCATAACTCTGGCTAACGAAGCGGGCGGGATTGGCATc                                                                                                                 <  2:3468062/148‑1 (MQ=255)
                               cGTCTCACTGGTGCGCCACACGGATGGCACGCCGCTCTATTTTATCGCTCAGATTGAAGACATTAACGAGCTAAAACGCACCGAACAGGTCAATCAGCAACTGATGGAGCGCATAACTCTGGCTAACGAAGCGGGCGGGATTGGCATc                                                                                                                 <  2:78276/148‑1 (MQ=255)
                                gTCTCACTGGTGCGCCACACGGATGGCACGCCGCTCTATTTTATCGCTCAGATTGAAGACATTAACGAGCTAAAACGCACCGAACAGGTCAATCAGCAACTGATGGAGCGCATAACTCTGGCTAACGAAGCGGGCGGGATTGGCATCt                                                                                                                <  2:1402947/148‑1 (MQ=255)
                                  ctcACTGGTGCGCCACACGGATGGCACGCCGCTCTATTTTATCGCTCAGATTGAAGACATTAACGAGCTAAAACGCACCGAACAGGTCAATCAGCAACTGATGGAGCGCATAACTCTGGCTAACGAAGCGGGCGGGATTGGCATCtgg                                                                                                              >  2:469841/1‑148 (MQ=255)
                                   tcACTGGTGCGCCACACGGATGGCACGCCGCTCTATTTTATCGCTCAGATTGAAGACATTAACGAGCTAAAACGCACCGAACAGGTCAATCAGCAACTGATGGAGCGCATAACTCTGGCTAACGAAGCGGGCGGGATTGGCATCtggg                                                                                                             >  1:1064902/1‑148 (MQ=255)
                                     aCTGGTGCGCCACACGGATGGCACGCCGCTCTATTTTATCGCTCAGATTGAAGACATTAACGAGCTAAAACGCACCGAACAGGTCAATCAGCAACTGATGGAGCGCATAACTCTGGCTAACGAAGCGGGCGGGATTGGCATCtgggag                                                                                                           >  1:2625472/1‑148 (MQ=255)
                                     aCTGGTGCGCCACACGGATGGCACGCCGCTCTATTTTATCGCTCAGATTGAAGACATTAACGAGCTAAAACGCACCGAACAGGTCAATCAGCAACTGATGGAGCGCATAACTCTGGCTAACGAAGCGGGCGGGATTGGCATCtgggag                                                                                                           >  1:3046340/1‑148 (MQ=255)
                                     aCTGGTGCGCCACACGGATGGCACGCCGCTCTATTTTATCGCTCAGATTGAAGACATTAACGAGCTAAAACGCACCGAACAGGTCAATCAGCAACTGATGGAGCGCATAACTCTGGCTAACGAAGCGGGCGGGATTGGCATCtgggag                                                                                                           >  2:3473503/1‑148 (MQ=255)
                                       tGGTGCGCCACACGGATGGCACGCCGCTCTATTTTATCGCTCAGATTGAAGACATTAACGAGCTAAAACGCACCGAACAGGTCAATCAGCAACTGATGGAGCGCATAACTCTGGCTAACGAAGCGGGCGGGATTGGCATCtgggagtg                                                                                                         <  1:3373168/148‑1 (MQ=255)
                                       tGGTGCGCCACACGGATGGCACGCCGCTCTATTTTATCGCTCAGATTGAAGACATTAACGAGCTAAAACGCACCGAACAGGTCAATCAGCAACTGATGGAGCGCATAACTCTGGCTAACGAAGCGGGCGGGATTGGCATCtgggagtg                                                                                                         >  2:552631/1‑148 (MQ=255)
                                       tGGTGCGCCACACGGATGGCACGCCGCTCTATTTTATCGCTCAGATTGAAGACATTAACGAGCTAAAACGCACCGAACAGGTCAATCAGCAACTGATGGAGCGCATAACTCTGGCTAACGAAGCGGGCGGGATTGGCATCtgggagtg                                                                                                         >  2:1666801/1‑148 (MQ=255)
                                            cgcCACACGGATGGCACGCCGCTCTATTTTATCGCTCAGATTGAAGACATTAACGAGCTAAAACGCACCGAACAGGTCAATCAGCAACTGATGGAGCGCATAACTCTGGCTAACGAAGCGGGCGGGATTGGCATCTgggagtgggagt                                                                                                    <  1:168022/148‑1 (MQ=255)
                                              ccACACGGATGGCACGCCGCTCTATTTTATCGCTCAGATTGAAGACATTAACGAGCTAAAACGCACCGAACAGGTCAATCAGCAACTGATGGAGCGCATAACTCTGGCTAACGAAGCGGGCGGGATTGGCATCTGGGAGTGGGAGTTg                                                                                                  <  2:262034/148‑1 (MQ=255)
                                              ccACACGGATGGCACGCCGCTCTATTTTATCGCTCAGATTGAAGACATTAACGAGCTAAAACGCACCGAACAGGTCAATCAGCAACTGATGGAGCGCATAACTCTGGCTAACGAAGCGGGCGGGATTGGCATCTGGGAGTGGGAGTTg                                                                                                  <  1:2644908/148‑1 (MQ=255)
                                              ccACACGGATGGCACGCCGCTCTATTTTATCGCTCAGATTGAAGACATTAACGAGCTAAAACGCACCGAACAGGTCAATCAGCAACTGATGGAGCGCATAACTCTGGCTAACGAAGCGGGCGGGATTGGCATCTGGGAGTGGGAGTTg                                                                                                  <  2:2501566/148‑1 (MQ=255)
                                               cacaCGGATGGCACGCCGCTCTATTTTATCGCTCAGATTGAAGACATTAACGAGCTAAAACGCACCGAACAGGTCAATCAGCAACTGATGGAGCGCATAACTCTGGCTAACGAAGCGGGCGGGATTGGCATCTGGGAGTGGGAGTTGa                                                                                                 <  1:202061/148‑1 (MQ=255)
                                                acacGGATGGCACGCCGCTCTATTTTATCGCTCAGATTGAAGACATTAACGAGCTAAAACGCACCGAACAGGTCAATCAGCAACTGATGGAGCGCATAACTCTGGCTAACGAAGCGGGCGGGATTGGCATCTGGGAGTGGGAGTTGaa                                                                                                <  1:653601/148‑1 (MQ=255)
                                                acacGGATGGCACGCCGCTCTATTTTATCGCTCAGATTGAAGACATTAACGAGCTAAAACGCACCGAACAGGTCAATCAGCAACTGATGGAGCGCATAACTCTGGCTAACGAAGCGGGCGGGATTGGCATCTGGGAGTGGGAGTTGaa                                                                                                <  1:821750/148‑1 (MQ=255)
                                                acacGGATGGCACGCCGCTCTATTTTATCGCTCAGATTGAAGACATTAACGAGCTAAAACGCACCGAACAGGTCAATCAGCAACTGATGGAGCGCATAACTCTGGCTAACGAAGCGGGCGGGATTGGCATCTGGGAGTGGGAGTTGaa                                                                                                >  1:1316359/1‑148 (MQ=255)
                                                acacGGATGGCACGCCGCTCTATTTTATCGCTCAGATTGAAGACATTAACGAGCTAAAACGCACCGAACAGGTCAATCAGCAACTGATGGAGCGCATAACTCTGGCTAACGAAGCGGGCGGGATTGGCATCTGGGAGTGGGAGTTGaa                                                                                                <  1:2415066/148‑1 (MQ=255)
                                                  acGGATGGCACGCCGCTCTATTTTATCGCTCAGATTGAAGACATTAACGAGCTAAAACGCACCGAACAGGTCAATCAGCAACTGATGGAGCGCATAACTCTGGCTAACGAAGCGGGCGGGATTGGCATCTGGGAGTGGGAGTTGAAGc                                                                                              <  2:2941824/148‑1 (MQ=255)
                                                   cGGATGGCACGCCGCTCTATTTTATCGCTCAGATTGAAGACATTAACGAGCTAAAACGCACCGAACAGGTCAATCAGCAACTGATGGAGCGCATAACTCTGGCTAACGAAGCGGGCGGGATTGGCATCTGGGAGTGGGAGTTGAAGcc                                                                                             <  1:3100548/148‑1 (MQ=255)
                                                    ggATGGCACGCCGCTCTATTTTATCGCTCAGATTGAAGACATTAACGAGCTAAAACGCACCGAACAGGTCAATCAGCAACTGATGGAGCGCATAACTCTGGCTAAAGAAGCGGGCGGGATTGGCATCTGGGAGTGGGAGTTGAAGCCg                                                                                            >  1:1487883/1‑148 (MQ=255)
                                                       tGGCACGCCGCTCTATTTTATCGCTCAGATTGAAGACATTAACGAGCTAAAACGCACCGAACAGGTCAATCAGCAACTGATGGAGCGCATAACTCTGGCTAACGAAGCGGGCGGGATTGGCATCTGGGAGTGGGAGTTGAAGCCGAat                                                                                         <  2:2363357/148‑1 (MQ=255)
                                                        ggCACGCCGCTCTATTTTATCGCTCAGATTGAAGACATTAACGAGCTAAAACGCACCGAACAGGTCAATCAGCAACTGATGGAGCGCATAACTCTGGCTAACGAAGCGGGCGGGATTGGCATCTGGGAGTGGGAGTTGAAGCCGAata                                                                                        <  1:3356303/148‑1 (MQ=255)
                                                         gCACGCCGCTCTATTTTATCGCTCAGATTGAAGACATTAACGAGCTAAAACGCACCGAACAGGTCAATCAGCAACTGATGGAGCGCATAACTCTGGCTAACGAAGCGGGCGGGATTGGCATCTGGGAGTGGGAGTTGAAGCCGAatat                                                                                       <  2:1755444/148‑1 (MQ=255)
                                                            cgccgcTCTATTTTATCGCTCAGATTGAAGACATTAACGAGCTAAAACGCACCGAACAGGTCAATCAGCAACTGATGGAGCGCATAACTCTGGCTAACGAAGCGGGCGGGATTGGCATCTGGGAGTGGGAGTTGAAGCCGAATAtttt                                                                                    >  2:818405/1‑148 (MQ=255)
                                                             gccgcTCTATTTTATCGCTCAGATTGAAGACATTAACGAGCTAAAACGCACCGAACAGGTCAATCAGCAACTGATGGAGCGCATAACTCTGGCTAACGAAGCGGGCGGGATTGGCATCTGGGAGTGGGAGTTGAAGCCGAATAttttt                                                                                   <  1:1552502/148‑1 (MQ=255)
                                                             gccgcTCTATTTTATCGCTCAGATTGAAGACATTAACGAGCTAAAACGCACCGAACAGGTCAATCAGCAACTGATGGAGCGCATAACTCTGGCTAACGAAGCGGGCGGGATTGGCATCTGGGAGTGGGAGTTGAAGCCGAATAttttt                                                                                   <  2:876745/148‑1 (MQ=255)
                                                               cgcTCTATTTTATCGCTCAGATTGAAGACATTAACGAGCTAAAACGCACCGAACAGGTCAATCAGCAACTGATGGAGCGCATAACTCTGGCTAACGAAGCGGGCGGGATTGGCATCTGGGAGTGGGAGTTGAAGCCGAATATTTTTAg                                                                                 <  2:3149618/148‑1 (MQ=255)
                                                                 ctctATTTTATCGCTCAGATTGAAGACATTAACGAGCTAAAACGCACCGAACAGGTCAATCAGCAACTGATGGAGCGCATAACTCTGGCTAACGAAGCGGGCGGGATTGGCATCTGGGAGTGGGAGTTGAAGCCGAATATTTTTAGCt                                                                               <  1:522449/148‑1 (MQ=255)
                                                                  tctATTTTATCGCTCAGATTGAAGACATTAACGAGCTAAAACGCACCGAACAGGTCAATCAGCAACTGATGGAGCGCATAACTCTGGCTAACGAAGCGGGCGGGATTGGCATCTGGGAGTGGGAGTTGAAGCCGAATATTTTTAGCTg                                                                              >  1:2909300/1‑148 (MQ=255)
                                                                     aTTTTATCGCTCAGATTGAAGACATTAACGAGCTAAAACGCACCGAACAGGTCAATCAGCAACTGATGGAGCGCATAACTCTGGCTAACGAAGCGGGCGGGATTGGCATCTGGGAGTGGGAGTTGAAGCCGAATATTTTTAGCTGGGa                                                                           >  1:704486/1‑148 (MQ=255)
                                                                       tttATCGCTCAGATTGAAGACATTAACGAGCTAAAACGCACCGAACAGGTCAATCAGCAACTGATGGAGCGCATAACTCTGGCTAACGAAGCGGGCGGGATTGGCATCTGGGAGTGGGAGTTGAAGCCGAATATTTTTAGCTGGGATa                                                                         >  2:1312449/1‑148 (MQ=255)
                                                                          aTCGCTCAGATTGAAGACATTAACGAGCTAAAACGCACCGAACAGGTCAATCAGCAACTGATGGAGCGCATAACTCTGGCTAACGAAGCGGGCGGGATTGGCATCTGGGAGTGGGAGTTGAAGCCGAATATTTTTAGCTGGGATAAGc                                                                      >  2:1510974/1‑148 (MQ=255)
                                                                            cGCTCAGATTGAAGACATTAACGAGCTAAAACGCACCGAACAGGTCAATCAGCAACTGATGGAGCGCATAACTCTGGCTAACGAAGCGGGCGGGATTGGCATCTGGGAGTGGGAGTTGAAGCCGAATATTTTTAGCTGGGATAAGCgg                                                                    >  2:1668470/1‑148 (MQ=255)
                                                                               tCAGATTGAAGACATTAACGAGCTAAAACGCACCGAACAGGTCAATCAGCAACTGATGGAGCGCATAACTCTGGCTAACGAAGCGGGCGGGATTGGCATCTGGGAGTGGGAGTTGAAGCCGAATATTTTTAGCTGGGATAAGCGGATg                                                                 >  2:1698372/1‑148 (MQ=255)
                                                                                cAGATTGAAGACATTAACGAGCTAAAACGCACCGAACAGGTCAATCAGCAACTGATGGAGCGCATAACTCTGGCTAACGAAGCGGGCGGGATTGGCATCTGGGAGTGGGAGTTGAAGCCGAATATTTTTAGCTGGGCTAAGCGGATGt                                                                <  1:745067/148‑1 (MQ=255)
                                                                                cAGATTGAAGACATTAACGAGCTAAAACGCACCGAACAGGTCAATCAGCAACTGATGGAGCGCATAACTCTGGCTAACGAAGCGGGCGGGATTGGCATCTGGGAGTGGGAGTTGAAGCCGAATATTTTTAGCTGGGATAAGCGGATGt                                                                <  2:1348403/148‑1 (MQ=255)
                                                                                   aTTGAAGACATTAACGAGCTAAAACGCACCGAACAGGTCAATCAGCAACTGATGGAGCGCATAACTCTGGCTAACGAAGCGGGCGGGATTGGCATCTGGGAGTGGGAGTTGAAGCCGAATATTTTTAGCTGGGATAAGCGGATGTTCg                                                             <  2:3141246/148‑1 (MQ=255)
                                                                                    ttGAAGACATTAACGAGCTAAAACGCACCGAACAGGTCAATCAGCAACTGATGGAGCGCATAACTCTGGCTAACGAAGCGGGCGGGATTGGCATCTGGGAGTGGGAGTTGAAGCCGAATATTTTTAGCTGGGATAAGCGGATGTTCGa                                                            >  1:1439480/1‑148 (MQ=255)
                                                                                    ttGAAGACATTAACGAGCTAAAACGCACCGAACAGGTCAATCAGCAACTGATGGAGCGCATAACTCTGGCTAACGAAGCGGGAGGGATTGGCATCTGGGAGTGGGAGTTGAAGCCGAATATTTTTAGCTGGTATAAGCGGATGTTCGa                                                            >  2:3414117/1‑148 (MQ=255)
                                                                                     tGAAGACATTAACGAGCTAAAACGCACCGAACAGGTCAATCAGCAACTGATGGAGCGCATAACTCTGGCTAACGAAGCGGGCGGGATTGGCATCTGGGAGTGGGAGTTGAAGCCGAATATTTTTAGCTGGGATAAGCGGATGTTCGAg                                                           >  1:781278/1‑148 (MQ=255)
                                                                                      gAAGACATTAACGAGCTAAAACGCACCGAACAGGTCAATCAGCAACTGATGGAGCGCATAACTCTGGCTAACGAAGCGGGCGGGATTGGCATCTGGGAGTGGGAGTTGAAGCCGAATATTTTTAGCTGGGATAAGCGGATGTTCGAGc                                                          >  1:2463879/1‑148 (MQ=255)
                                                                                        aGACATTAACGAGCTAAAACGCACCGAACAGGTCAATCAGCAACTGATGGAGCGCATAACTCTGGCTAACGAAGCGGGCGGGATTGGCATCTGGGAGTGGGAGTTGAAGCCGAATATTTTTAGCTGGGATAAGCGGATGTTCGAGCTg                                                        >  2:679649/1‑148 (MQ=255)
                                                                                        aGACATTAACGAGCTAAAACGCACCGAACAGGTCAATCAGCAACTGATGGAGCGCATAACTCTGGCTAACGAAGCGGGCGGGATTGGCATCTGGGAGTGGGAGTTGAAGCCGAATATTTTTAGCTGGGATAAGCGGATGTTCGAGCTg                                                        <  2:2528021/148‑1 (MQ=255)
                                                                                        aGACATTAACGAGCTAAAACGCACCGAACAGGTCAATCAGCAACTGATGGAGCGCATAACTCTGGCTAACGAAGCGGGCGGGATTGGCATCTGGGAGTGGGAGTTGAAGCCGAATATTTTTAGCTGGGATAAGCGGATGTTCGAGCTg                                                        >  2:687463/1‑148 (MQ=255)
                                                                                        aGACATTAACGAGCTAAAACGCACCGAACAGGTCAATCAGCAACTGATGGAGCGCATAACTCTGGCTAACGAAGCGGGCGGGATTGGCATCTGGGAGTGGGAGTTGAAGCCGAATATTTTTAGCTGGGATAAGCGGATGTTCGAGCTg                                                        >  2:485967/1‑148 (MQ=255)
                                                                                          aCATTAACGAGCTAAAACGCACCGAACAGGTCAATCAGCAACTGATGGAGCGCATAACTCTGGCTAACGAAGCGGGCGGGATTGGCATCTGGGAGTGGGAGTTGAAGCCGAATATTTTTAGCTGGGATAAGCGGATGTTCGAGCTGTa                                                      <  2:222037/148‑1 (MQ=255)
                                                                                          aCATTAACGAGCTAAAACGCACCGAACAGGTCAATCAGCAACTGATGGAGCGCATAACTCTGGCTAACGAAGCGGGCGGGATTGGCATCTGGGAGTGGGAGTTGAAGCCGAATATTTTTAGCTGGGATAAGCGGATGTTCGAGCTGTa                                                      >  1:5779/1‑148 (MQ=255)
                                                                                            aTTAACGAGCTAAAACGCACCGAACAGGTCAATCAGCAACTGATGGAGCGCATAACTCTGGCTAACGAAGCGGGCGGGATTGGCATCTGGGAGTGGGAGTTGAAGCCGAATATTTTTAGCTGGGATAAGCGGATGTTCGAGCTGTATg                                                    >  2:2714412/1‑148 (MQ=255)
                                                                                              tAACGAGCTAAAACGCACCGAACAGGTCAATCAGCAACTGATGGAGCGCATAACTCTGGCTAACGAAGCGGGCGGGATTGGCATCTGGGAGTGGGAGTTGAAGCCGAATATTTTTAGCTGGGATAAGCGGATGTTCGAGCTGTATGaa                                                  >  1:215762/1‑148 (MQ=255)
                                                                                              tAACGAGCTAAAACGCACCGAACAGGTCAATCAGCAACTGATGGAGCGCATAACTCTGGCTAACGAAGCGGGCGGGATTGGCATCTGGGAGTGGGAGTTGAAGCCGAATATTTTTAGCTGGGATAAGCGGATGTTCGAGCTGTATGaa                                                  >  1:991916/1‑148 (MQ=255)
                                                                                              tAACGAGCTAAAACGCACCGAACAGGTCAATCAGCAACTGATGGAGCGCATAACTCTGGCTAACGAAGCGGGCGGGATTGGCATCTGGGAGTGGGAGTTGAAGCCGAATATTTTTAGCTGGGATAAGCGGATGTTCGAGCTGTATGaa                                                  >  1:2745879/1‑148 (MQ=255)
                                                                                               aaCGAGCTAAAACGCACCGAACAGGTCAATCAGCAACTGATGGAGCGCATAACTCTGGCTAACGAAGCGGGCGGGATTGGCATCTGGGAGTGGGAGTTGAAGCCGAATATTTTTAGCTGGGATAAGCGGATGTTCGAGCTGTATGaaa                                                 <  2:110085/148‑1 (MQ=255)
                                                                                                aCGAGCTAAAACGCACCGACCAGGTCCCTCAGCAACTGAGGGAGCGCATAACTCTGGCTAACGAAGCGGGCGGGATTGGCATCTGGGAGTGGGAGTTGAAGCCGAATATTTTTAGCTGGGATAAGCGGATGTTCGAGCTGTATGAAAt                                                <  1:2672746/148‑1 (MQ=255)
                                                                                                   aGCTAAAACGCACCGAACAGGTCAATCAGCAACTGATGGAGCGCATAACTCTGGCTAACGAAGCGGGCGGGATTGGCATCTGGGAGTGGGAGTTGAAGCCGAATATTTTTAGCTGGGATAAGCGGATGTTCGAGCTGTATGAAATtcc                                             >  2:3300773/1‑148 (MQ=255)
                                                                                                     cTAAAACGCACCGAACAGGTCAATCAGCAACTGATGGAGCGCATAACTCTGGCTAACGAAGCGGGCGGGATTGGCATCTGGGAGTGGGAGTTGAAGCCGAATATTTTTAGCTGGGATAAGCGGATGTTCGAGCTGTATGAAATtcctc                                           <  1:978762/148‑1 (MQ=255)
                                                                                                     cTAAAACGCACCGAACAGGTCAATCAGCAACTGATGGAGCGCATAACTCTGGCTAACGAAGCGGGCGGGATTGGCATCTGGGAGTGGGAGTTGAAGCCGAATATTTTTAGCTGGGATAAGCGGATGTTCGAGCTGTATGAAATtcctc                                           <  2:308881/148‑1 (MQ=255)
                                                                                                     cTAAAACGCACCGAACAGGTCAATCAGCAACTGATGGAGCGCATAACTCTGGCTAACGAAGCGGGCGGGATTGGCATCTGGGAGTGGGAGTTGAAGCCGAATATTTTTAGCTGGGATAAGCGGATGTTCGAGCTGTATGAAATtcctc                                           <  2:308796/148‑1 (MQ=255)
                                                                                                        aaaCGCACCGAACAGGTCAATCAGCAACTGATGGAGCGCATAACTCTGGCTAACGAAGCGGGCGGGATTGGCATCTGGGAGTGGGAGTTGAAGCCGAATATTTTTAGCTGGGATAAGCGGATGTTCGAGCTGTATGAAATTCCTCcac                                        >  2:2283999/1‑148 (MQ=255)
                                                                                                         aaCGCACCGAACAGGTCAATCAGCAACTGATGGAGCGCATAACTCTGGCTAACGAAGCGGGCGGGATTGGCATCTGGGAGTGGGAGTTGAAGCCGAATATTTTTAGCTGGGATAAGCGGATGTTCGAGCTGTATGAAATTCCTCcaca                                       >  2:265029/1‑148 (MQ=255)
                                                                                                         aaCGCACCGAACAGGTCAATCAGCAACTGATGGAGCGCATAACTCTGGCTAACGAAGCGGGCGGGATTGGCATCTGGGAGTGGGAGTTGAAGCCGAATATTTTTAGCTGGGATAAGCGGATGTTCGAGCTGTATGAAATTCCTCcaca                                       >  2:1575390/1‑148 (MQ=255)
                                                                                                          aCGCACCGAACAGGTCAATCAGCAACTGATGGAGCGCATAACTCTGGCTAACGAAGCGGGCGGGATTGGCATCTGGGAGTGGGAGTTGAAGCCGAATATTTTTAGCTGGGATAAGCGGATGTTCGAGCTGTATGAAATTCCTCCACat                                      >  1:1574142/1‑148 (MQ=255)
                                                                                                            gCACCGAACAGGTCAATCAGCAACTGATGGAGCGCATAACTCTGGCTAACGAAGCGGGCGGGATTGGCATCTGGGAGTGGGAGTTGAAGCCGAATATTTTTAGCTGGGATAAGCGGATGTTCGAGCTGTATGAAATTCCTCCACatat                                    >  2:3463991/1‑148 (MQ=255)
                                                                                                             cACCGAACAGGTCAATCAGCAACTGATGGAGCGCATAACTCTGGCTAACGAAGCGGGCGGGATTGGCATCTGGGAGTGGGAGTTGAAGCCGAATATTTTTAGCTGGGATAAGCGGATGTTCGAGCTGTATGAAATTCCTCCACATATc                                   >  1:1002672/1‑148 (MQ=255)
                                                                                                               ccGAACAGGTCAATCAGCAACTGATGGAGCGCATAACTCTGGCTAACGAAGCGGGCGGGATTGGCATCTGGGAGTGGGAGTTGAAGCCGAATATTTTTAGCTGGGATAAGCGGATGTTCGAGCTGTATGAAATTCCTCCACATATCaa                                 >  1:936593/1‑148 (MQ=255)
                                                                                                                    cAGGTCAATCAGCAACTGATGGAGCGCATAACTCTGGCTAACGAAGCGGGCGGGATTGGCATCTGGGAGTGGGAGTTGAAGCCGAATATTTTTAGCTGGGATAAGCGGATGTTCGAGCTGTATGAAATTCCTCCACATATCAAACCGa                            <  2:515568/148‑1 (MQ=255)
                                                                                                                    cAGGTCAATCAGCAACTGATGGAGCGCATAACTCTGGCTAACGAAGCGGGCGGGATTGGCATCTGGGAGTGGGAGTTGAAGCCGAATATTTTTAGCTGGGATAAGCGGATGTTCGAGCTGTATGAAATTCCTCCACATATCAAACCGa                            >  1:468804/1‑148 (MQ=255)
                                                                                                                     aGGTCAATCAGCAACTGATGGAGCGCATAACTCTGGCTAACGAAGCGGGCGGGATTGGCATCTGGGAGTGGGAGTTGAAGCCGAATATTTTTAGCTGGGATAAGCGGATGTTCGAGCTGTATGAAATTCCTCCACATATCAAACCGaa                           >  1:2871744/1‑148 (MQ=255)
                                                                                                                      ggTCAATCAGCAACTGATGGAGCGCATAACTCTGGCTAACGAAGCGGGCGGGATTGGCATCTGGGAGTGGGAGTTGAAGCCGAATATTTTTAGCTGGGATAAGCGGATGTTCGAGCTGTATGAAATTCCTCCACATATCAAACCGAAc                          >  2:2164301/1‑148 (MQ=255)
                                                                                                                      ggTCAATCAGCAACTGATGGAGCGCATAACTCTGGCTAACGAAGCGGGCGGGATTGGCATCTGGGAGTGGGAGTTGAAGCCGAATATTTTTAGCTGGGATAAGCGGATGTTCGAGCTGTATGAAATTCCTCCACATATCAAAACGAAc                          >  2:2536752/1‑148 (MQ=255)
                                                                                                                       gTCAATCAGCAACTGATGGAGCGCATAACTCTGGCTAACGAAGCGGGCGGGATTGGCATCTGGGAGTGGGAGTTGAAGCCGAATATTTTTAGCTGGGATAAGCGGATGTTCGAGCTGTATGAAATTCCTCCACATATCAAACCGAACt                         <  1:429129/148‑1 (MQ=255)
                                                                                                                         cAATCAGCAACTGATGGAGCGCATAACTCTGGCTAACGAAGCGGGCGGGATTGGCATCTGGGAGTGGGAGTTGAAGCCGAATATTTTTAGCTGGGATAAGCGGATGTTCGAGCTGTATGAAATTCCTCCACATATCAAACCGAACTgg                       >  2:979862/1‑148 (MQ=255)
                                                                                                                            tCAGCAACTGATGGAGCGCATAACTCTGGCTAACGAAGCGGGCGGGATTGGCATCTGGGAGTGGGAGTTGAAGCCGAATATTTTTAGCTGGGATAAGCGGATGTTCGAGCTGTATGAAATTCCTCCACATATCAAACCGAACTGGCAg                    >  2:2531809/1‑148 (MQ=255)
                                                                                                                              aGCAACTGATGGAGCGCATAACTCTGGCTAACGAAGCGGGCGGGATTGGCATCTGGGAGTGGGAGTTGAAGCCGAATATTTTTAGCTGGGATAAGCGGATGTTCGAGCTGTATGAAATTCCTCCACATATCAAACCGAACTGGCAGgt                  <  2:1454412/148‑1 (MQ=255)
                                                                                                                               gCATCTGATGGAGCGCATAACTCTGGCTAACGAAGCGGGCGGGATTGGCATCTGGGAGTGGGAGTTGAAGCCGAATATTTTTAGCTGGGATAAGCGGATGTTCGAGCTGTATGAAATTCCTCCACATATCAAACCGAACTGGCAGgtg                 <  1:3326470/148‑1 (MQ=255)
                                                                                                                                cAACTGATGGAGCGCATAACTCTGGCTAACGAAGCGGGCGGGATTGGCATCTGGGAGTGGGAGTTGAAGCCGAATATTTTTAGCTGGGATAAGCGGATGTTCGAGCTGTATGAAATTCCTCCACATATCAAACCGAACTGGCAGgtgt                >  1:917339/1‑148 (MQ=255)
                                                                                                                                cAACTGATGGAGCGCATAACTCTGGCTAACGAAGCGGGCGGGATTGGCATCTGGGAGTGGGAGTTGAAGCCGAATATTTTTAGCTGGGATAAGCGGATGTTCGAGCTGTATGAAATTCCTCCACATATCAAACCGAACTGGCAGgtgt                >  1:2649467/1‑148 (MQ=255)
                                                                                                                                 aaCTGATGGAGCGCATAACTCTGGCTAACGAAGCGGGCGGGATTGGCATCTGGGAGTGGGAGTTGAAGCCGAATATTTTTAGCTGGGATAAGCGGATGTTCGAGCTGTATGAAATTCCTCCACATATCAAACCGAACTGGCAGGtgtg               >  2:3054120/1‑148 (MQ=255)
                                                                                                                                   cTGATGGAGCGCATAACTCTGGCTAACGAAGCGGGCGGGATTGGCATCTGGGAGTGGGAGTTGAAGCCGAATATTTTTAGCTGGGATAAGCGGATGTTCGAGCTGTATGAAATTCCTCCACATATCAAACCGAACTGGCAGGTGTGGt             >  1:1860793/1‑148 (MQ=255)
                                                                                                                                   cTGATGGAGCGCATAACTCTGGCTAACGAAGCGGGCGGGATTGGCATCTGGGAGTGGGAGTTGAAGCCGAATATTTTTAGCTGGGATAAGCGGATGTTCGAGCTGTATGAAATTCCTCCACATATCAAACCGAACTGGCAGGTGTGGt             >  2:1842695/1‑148 (MQ=255)
                                                                                                                                      aTGGAGCGCATAACTCTGGCTAACGAAGCGGGCGGGATTGGCATCTGGGAGTGGGAGTTGAAGCCGAATATTTTTAGCTGGGATAAGCGGATGTTCGAGCTGTATGAAATTCCTCCACATATCAAACCGAACTGGCAGGTGTGGTACg          >  1:1127990/1‑148 (MQ=255)
                                                                                                                                       tGGAGCGCATAACTCTGGCTAACGAAGCGGGCGGGATTGGCATCTGGGAGTGGGAGTTGAAGCCGAATATTTTTAGCTGGGATAAGCGGATGTTCGAGCTGTATGAAATTCCTCCACATATCAAACCGAACTGGCAGGTGTGGTACGa         <  2:418281/148‑1 (MQ=255)
                                                                                                                                       tGGAGCGCATAACTCTGGCTAACGAAGCGGGCGGGATTGGCATCTGGGAGTGGGAGTTGAAGCCGAATATTTTTAGCTGGGATAAGCGGATGTTCGAGCTGTATGAAATTCCTCCACATATCAAACCGAACTGGCAGGTGTGGTACGa         <  1:245571/148‑1 (MQ=255)
                                                                                                                                         gAGCGCATAACTCTGGCTAACGAAGCGGGCGGGATTGGCATCTGGGAGTGGGAGTTGAAGCCGAATATTTTTAGCTGGGATAAGCGGATGTTCGAGCTGTATGAAATTCCTCCACATATCAAACCGAACTGGCAGGTGTGGTACGAgt       <  1:1650048/148‑1 (MQ=255)
                                                                                                                                            cgcATAACTCTGGCTAACGAAGCGGGCGGGATTGGCATCTGGGTGTGGGAGTTGAAGCCGAATATTTTTAGCTGGGATAAGCGGATGTTCGAGCTGTATGAAATTCCTCCACATATCAAACCGAACTGGCAGGTGTGGTACGAgtgcg    <  1:1916623/148‑1 (MQ=255)
                                                                                                                                            cgcATAACTCTGGCTAACGAAGCGGGCGGGATTGGCATCTGGGAGTGGGAGTTGAAGCCGAATATTTTTAGCTGGGATAAGCGGATGTTCGAGCTGTATGAAATTCCTCCACATATCAAACCGAACTGGCAGGTGTGGTACGAgtgcg    <  1:3299977/148‑1 (MQ=255)
                                                                                                                                              cATAACTCTGGCTAACGAAGCGGGCGGGATTGGCATCTGGGAGTGGGAGTTGAAGCCGAATATTTTTAGCTGGGATAAGCGGATGTTCGAGCTGTATGAAATTCCTCCACATATCAAACCGAACTGGCAGGTGTGGTACGAgtgcgtg  <  1:3473503/148‑1 (MQ=255)
                                                                                                                                              cATAACTCTGGCTAACGAAGCGGGCGGGATTGGCATCTGGGAGTGGGAGTTGAAGCCGAATATTTTTAGCTGGGATAAGCGGATGTTCGAGCTGTATGAAATTCCTCCACATATCAAACCGAACTGGCAGGTGTGGTACGAgtgcgtg  <  1:3035906/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
GCAATGGCGATGTTGTCTGGGCGTTGCTTGCCGTCTCACTGGTGCGCCACACGGATGGCACGCCGCTCTATTTTATCGCTCAGATTGAAGACATTAACGAGCTAAAACGCACCGAACAGGTCAATCAGCAACTGATGGAGCGCATCACTCTGGCTAACGAAGCGGGCGGGATTGGCATCTGGGAGTGGGAGTTGAAGCCGAATATTTTTAGCTGGGATAAGCGGATGTTCGAGCTGTATGAAATTCCTCCACATATCAAACCGAACTGGCAGGTGTGGTACGAGTGCGTG  >  CP009273/2137894‑2138183

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
ACCGCAATGGCGATGTTGTCTGGGCGTTGCTTGCCGTCTCACTGGTGCGCCACACGGATGGCACGCCGCTCTATTTTATCGCTCAGATTGAAGACATTAACGAGCTAAAACGCACCGAACAGGTCAATCAGCAACTGATGGAGCGCATCACTCTGGCTAACGAAGCGGGCGGGATTGGCATCTGGGAGTGGGAGTTGAAGCCGAATATTTTTAGCTGGGATAAGCGGATGTTCGAGCTGTATGAAATTCCTCCACATATCAAACCGAACTGGCAGGTGTGGTACGAGTGCGTGCT  >  CP009273/2137891‑2138185
                                                                                                                                                    |                                                                                                                                                  
ACCGCAATGGCGATGTTGTCTGGGCGTTGCTTGCCGTCTCACTGGTGCGCCACACGGATGGCACGCCGCTCTATTTTATCGCTCAGATTGAAGACATTAACGAGCTAAAACGCACCGAACAGGTCAATCAGCAACTGATGGAGCGCATAA                                                                                                                                                   >  SRR19973065.2614468/1‑150 (MQ=60)
ACCGCAATGGCGATGTTGTCTGGGCGTTGCTTGCCGTCTCACTGGTGCGCCACACGGATGGCACGCCGCTCTATTTTATCGCTCAGATTGAAGACATTAACGAGCTAAAACGCACCGAACAGGTCAATCAGCAACTGATGGAGCGCATAA                                                                                                                                                   >  SRR19973065.1140455/1‑150 (MQ=60)
   GCAATGGCGATGTTGTCTGGGCGTTGCTTGCCGTCTCACTGGTGCGCCACACGGATGGCACGCCGCTCTATTTTATCGCTCAGATTGAAGACATTAACGAGCTAAAACGCACCGAACAGGTCAATCAGCAACTGATGGAGCGCATAACTC                                                                                                                                                >  SRR19973065.1766988/1‑150 (MQ=60)
   GCAATGGCGATGTTGTCTGGGCGTTGCTTGCCGTCTCACTGGTGCGCCACACGGATGGCACGCCGCTCTATTTTATCGCTCAGATTGAAGACATTAACGAGCTAAAACGAACCGAAAAGGTCAATCAGCACCTGATGGAGCGCATAACTC                                                                                                                                                >  SRR19973065.3383504/1‑150 (MQ=60)
    CAATGGCGATGTTGTCTGGGCGTTGCTTGCCGTCTCACTGGTGCGCCACACGGATGGCACGCCGCTCTATTTTATCGCTCAGATTGAAGACATTAACGAGCTAAAACGCACCGAACAGGTCAATCAGCAACTGATGGAGCGCATAACTCT                                                                                                                                               >  SRR19973065.1125336/1‑150 (MQ=60)
      ATGGCGATGTTGTCTGGGCGTTGCTTGCCGTCTCACTGGTGCGCCACACGGATGGCACGCCGCTCTATTTTATCGCTCAGATTGAAGACATTAACGAGCTAAAACGCACCGAACAGGTCAATCAGCAACTGATGGAGCGCATAACTCTGG                                                                                                                                             >  SRR19973065.2339785/1‑150 (MQ=60)
        GGCGATGTTGTCTGGGCGTTGCTTGCCGTCTCACTGGTGCGCCACACGGATGGCACGCCGCTCTATTTTATCGCTCAGATTGAAGACATTAACGAGCTAAAACGCACCGAACAGGTCAATCAGCAACTGATGGAGCGCATAACTCTGGCT                                                                                                                                           <  SRR19973065.407703/150‑1 (MQ=60)
         GCGATGTTGTCTGGGCGTTGCTTGCCGTCTCACTGGTGCGCCACACGGATGGCACGCCGCTCTATTTTATCGCTCAGATTGAAGACATTAACGAGCTAAAACGCACCGAACAGGTCAATCAGCAACTGATGGAGCGCATAACTCTGGCTA                                                                                                                                          <  SRR19973065.3488172/150‑1 (MQ=60)
         GCGATGTTGTCTGGGCGTTGCTTGCCGTCTCACTGGTGCGCCACACGGATGGCACGCCGCTCTATTTTATCGCTCAGATTGAAGACATTAACGAGCTAAAACGCACCGAACAGGTCAATCAGCAACTGATGGAGCGCATAACTCTGGCTA                                                                                                                                          <  SRR19973065.2361679/150‑1 (MQ=60)
         GCGATGTTGTCTGGGCGTTGCTTGCCGTCTCACTGGTGCGCCACACGGATGGCACGCCGCTCTATTTTATCGCTCAGATTGAAGACATTAACGAGCTAAAACGCACCGAACAGGTCAATCAGCAACTGATGGAGCGCATAACTCTGGCTA                                                                                                                                          <  SRR19973065.3488150/150‑1 (MQ=60)
          CGATGTTGTCTGGGCGTTGCTTGCCGTCTCACTGGTGCGCCACACGGATGGCACGCCGCTCTATTTTATCGCTCAGATTGAAGACATTAACGAGCTAAAACGCACCGAACAGGTCAATCAGCAACTGATGGAGCGCATAACTCTGGCTAA                                                                                                                                         >  SRR19973065.699708/1‑150 (MQ=60)
            ATGTTGTCTGGGCGTTGCTTGCCGTCTCACTGGTGCGCCACACGGATGGCACGCCGCTCTATTTTATCGCTCAGATTGAAGACATTAACGAGCTAAAACGCACCGAACAGGTCAATCAGCAACTGATGGAGCGCATAACTCTGGCTAACG                                                                                                                                       <  SRR19973065.2130929/150‑1 (MQ=60)
               TTGTCTGGGCGTTGCTTGCCGTCTCACTGGTGCGCCACACGGATGGCACGCCGCTCTATTTTATCGCTCAGATTGAAGACATTAACGAGCTAAAACGCACCGAACAGGTCAATCAGCAACTGATGGAGCGCATAACTCTGGCTAACGAAG                                                                                                                                    <  SRR19973065.1338905/150‑1 (MQ=60)
                TGTCTGGGCGTTGCTTGCCGTCTCACTGGTGCGCCACACGGATGGCACGCCGCTCTATTTTATCGCTCAGATTGAAGACATTAACGAGCTAAAACGCACCGAACAGGTCAATCAGCAACTGATGGAGCGCATAACTCTGGCTAACGAAGC                                                                                                                                   <  SRR19973065.1595143/150‑1 (MQ=60)
                       GCGTTGCTTGCCGTCTCACTGGTGCGCCACACGGATGGCACGCCGCTCTATTTTATCGCTCAGATTGAAGACATTAACGAGCTAAAACGCACCGAACAGGTCAATCAGCAACTGATGGAGCGCATAACTCTGGCTAACGAAGCGGGCGGG                                                                                                                            <  SRR19973065.245152/150‑1 (MQ=60)
                          TTGCTTGCCGTCTCACTGGTGCGCCACACGGATGGCACGCCGCTCTATTTTATCGCTCAGATTGAAGACATTAACGAGCTAAAACGCACCGAACAGGTCAATCAGCAACTGATGGAGCGCATAACTCTGGCTAACGAAGCGGGCGGGATT                                                                                                                         <  SRR19973065.447546/150‑1 (MQ=60)
                          TTGCTTGCCGTCTCACTGGTGCGCCACACGGATGGCACGCCGCTCTATCTTATCGCTCAGATTGAAGACATTAACGAGCTAAAACGCACCGAACAGGTCAATCAGCAACTGATGGAGCGCATAACTCTGGCTAACGAAGCGGGCGGGATT                                                                                                                         <  SRR19973065.447485/150‑1 (MQ=60)
                                   GTCTCACTGGTGCGCCACACGGATGGCACGCCGCTCTATTTTATCGCTCAGATTGAAGACATTAACGAGCTAAAACGCACCGAACAGGTCAATCAGCAACTGATGGAGCGCATAACTCTGGCTAACGAAGCGGGCGGGATTGGCATCTGG                                                                                                                <  SRR19973065.364760/150‑1 (MQ=60)
                                   GTCTCACTGGTGCGCCACACGGATGGCACGCCGCTCTATTTTATCGCTCAGATTGAAGACATTAACGAGCTAAAACGCACCGAACAGGTCAATCAGCAACTGATGGAGCGCATAACTCTGGCTAACGAAGCGGGCGGGATTGGCATCTGG                                                                                                                <  SRR19973065.2981795/150‑1 (MQ=60)
                                    TCTCACTGGTGCGCCACACGGATGGCACGCCGCTCTATTTTATCGCTCAGATTGAAGACATTAACGAGCTAAAACGCACCGAACAGGTCAATCAGCAACTGATGGAGCGCATAACTCTGGCTAACGAAGCGGGCGGGATTGGCATCTGGG                                                                                                               >  SRR19973065.1071965/1‑150 (MQ=60)
                                    TCTCACTGGTGCGCCACACGGATGGCACGCCGCTCTATTTTATCGCTCAGATTGAAGACATTAACGAGCTAAAACGCACCGAACAGGTCAATCAGCAACTGATGGAGCGCATAACTCTGGCTAACGAAGCGGGCGGGATTGGCATCTGGG                                                                                                               >  SRR19973065.1071955/1‑150 (MQ=60)
                                     CTCACTGGTGCGCCACACGGATGGCACGCCGCTCTATTTTATCGCTCAGATTGAAGACATTAACGAGCTAAAACGCACCGAACAGGTCAATCAGCAACTGATGGAGCGCATAACTCTGGCTAACGAAGCGGGCGGGATTGGCATCTGGGA                                                                                                              <  SRR19973065.1474337/150‑1 (MQ=60)
                                      TCACTGGTGCGCCACACGGATGGCACGCCGCTCTATTTTATCGCTCAGATTGAAGACATTAACGAGCTAAAACGCACCGAACAGGTCAATCAGCAACTGATGGAGCGCATAACTCTGGCTAACGAAGCGGGCGGGATTGGCATCTGGGAG                                                                                                             >  SRR19973065.2641714/1‑150 (MQ=60)
                                      TCACTGGTGCGCCACACGGATGGCACGCCGCTCTATTTTATCGCTCAGATTGAAGACATTAACGAGCTAAAACGCACCGAACAGGTCAATCAGCAACTGATGGAGCGCATAACTCTGGCTAACGAAGCGGGCGGGATTGGCATCTGGGAG                                                                                                             >  SRR19973065.2641724/1‑150 (MQ=60)
                                      TCACTGGTGCGCCACACGGATGGCACGCCGCTCTATTTTATCGCTCAGATTGAAGACATTAACGAGCTAAAACGCACCGAACAGGTCAATCAGCAACTGATGGAGCGCATAACTCTGGCTAACGAAGCGGGCGGGATTGGCATCTGGGAG                                                                                                             >  SRR19973065.3064981/1‑150 (MQ=60)
                                       CTCTGGTGCGCCACACGGATGGCACGCCGCTCTATTTTATCGCTCAGATTGTAGACATTAACGAGCTACAACGCACCGAACAGGTCACTCAGCTACTGATGGAGCGCTTAACTCTGGCTAACGTAGCGGGCGGGATTGGCATCTGGGTGT                                                                                                            <  SRR19973065.3515821/150‑1 (MQ=60)
                                         CTGGTGCGCCACACGGATGGCACGCCGCTCTATTTTATCGCTCAGATTGAAGACATTAACGAGCTAAAACGCACCGAACAGGTCAATCAGCAACTGATGGAGCGCATAACTCTGGCTAACGAAGCGGGCGGGATTGGCATCTGGGAGTGG                                                                                                          <  SRR19973065.1578398/150‑1 (MQ=60)
                                          TGGTGCGCCACACGGATGGCACGCCGCTCTATTTTATCGCTCAGATTGAAGACATTAACGAGCTAAAACGCACCGAACAGGTCAATCAGCAACTGATGGAGCGCATAACTCTGGCTAACGAAGCGGGCGGGATTGGCATCTGGGAGTGGG                                                                                                         <  SRR19973065.3393794/150‑1 (MQ=60)
                                            GTGCGCCACACGGATGGCACGCCGCTCTATTTTATCGCTCAGATTGAAGACATTAACGAGCTAAAACGCACCGAACAGGTCAATCAGCAACTGATGGAGCGCATAACTCTGGCTAACGAAGCGGGCGGGATTGGCATCTGGGAGTGGGAG                                                                                                       <  SRR19973065.2769486/150‑1 (MQ=60)
                                              GCGCCACACGGATGGCACGCCGCTCTATTTTATCGCTCAGATTGAAGACATTAACGAGCTAAAACGCACCGAACAGGTCAATCAGCAACTGATGGAGCGCATAACTCTGGCTAACGAAGCGGGCGGGATTGGCATCTGGGAGTGGGAGTT                                                                                                     <  SRR19973065.3165136/150‑1 (MQ=60)
                                               CGCCACACGGATGGCACGCCGCTCTATTTTATCGCTCAGATTGAAGACATTAACGAGCTAAAACGCACCGAACAGGTCAATCAGCAACTGATGGAGCGCATAACTCTGGCTAACGAAGCGGGCGGGATTGGCATCTGGGAGTGGGAGTTG                                                                                                    <  SRR19973065.169384/150‑1 (MQ=60)
                                               CGCCACACGGATGGCACGCCGCTCTATTTTATCGCTCAGATTGAAGACATTAACGAGCTAAAACGCACCGAACAGGTCAATCAGCAACTGATGGAGCGCATAACTCTGGCTAACGAAGCGGGCGGGATTGGCATCTGGGAGTGGGAGTTG                                                                                                    <  SRR19973065.169240/150‑1 (MQ=60)
                                                 CCACACGGATGGCACGCCGCTCTATTTTATCGCTCAGATTGAAGACATTAACGAGCTAAAACGCACCGAACAGGTCAATCAGCAACTGATGGAGCGCATAACTCTGGCTAACGAAGCGGGCGGGATTGGCATCTGGGAGTGGGAGTTGAA                                                                                                  <  SRR19973065.2661219/150‑1 (MQ=60)
                                                 CCACACGGATGGCACGCCGCTCTATTTTATCGCTCAGATTGAAGACATTAACGAGCTAAAACGCACCGAACAGGTCAATCAGCAACTGATGGAGCGCATAACTCTGGCTAACGAAGCGGGCGGGATTGGCATCTGGGAGTGGGAGTTGAA                                                                                                  >  SRR19973065.1325065/1‑150 (MQ=60)
                                                  CACACGGATGGCACGCCGCTCTATTTTATCGCTCAGATTGAAGACATTAACGAGCTAAAACGCACCGAACAGGTCAATCAGCAACTGATGGAGCGCATAACTCTGGCTAACGAAGCGGGCGGGATTGGCATCTGGGAGTGGGAGTTGAAG                                                                                                 <  SRR19973065.203618/150‑1 (MQ=60)
                                                  CACACGGATGGCACGCCGCTCTATTTTATCGCTCAGATTGAAGACATTAACGAGCTAAAACGCACCGAACAGGTCAATCAGCAACTGATGGAGCGCATAACTCTGGCTAACGAAGCGGGCGGGATTGGCATCTGGGAGTGGGAGTTGAAG                                                                                                 <  SRR19973065.305803/150‑1 (MQ=60)
                                                  CACACGGATGGCACGCCGCTCTATTTTATCGCTCAGATTGAAGACATTAACGAGCTAAAACGCACCGAACAGGTCAATCAGCAACTGATGGAGCGCATAACTCTGGCTAACGAAGCGGGCGGGATTGGCATCTGGGAGTGGGAGTTGAAG                                                                                                 >  SRR19973065.153390/1‑150 (MQ=60)
                                                   ACACGGATGGCACGCCGCTCTATTTTATCGCTCAGATTGAAGACATTAACGAGCTAAAACGCACCGAACAGGTCAATCAGCAACTGATGGAGCGCATAACTCTGGCTAACGAAGCGGGCGGGATTGGCATCTGGGAGTGGGAGTTGAAGC                                                                                                <  SRR19973065.1927331/150‑1 (MQ=60)
                                                   ACACGGATGGCACGCCGCTCTATTTTATCGCTCAGATTGAAGACATTAACGAGCTAAAACGCACCGAACAGGTCAATCAGCAACTGATGGAGCGCATAACTCTGGCTAACGAAGCGGGCGGGATTGGCATCTGGGAGTGGGAGTTGAAGC                                                                                                <  SRR19973065.827145/150‑1 (MQ=60)
                                                   ACACGGATGGCACGCCGCTCTATTTTATCGCTCAGATTGAAGACATTAACGAGCTAAAACGCACCGAACAGGTCAATCAGCAACTGATGGAGCGCATAACTCTGGCTAACGAAGCGGGCGGGATTGGCATCTGGGAGTGGGAGTTGAAGC                                                                                                <  SRR19973065.826885/150‑1 (MQ=60)
                                                   ACACGGATGGCACGCCGCTCTATTTTATCGCTCAGATTGAAGACATTAACGAGCTAAAACGCACCGAACAGGTCAATCAGCAACTGATGGAGCGCATAACTCTGGCTAACGAAGCGGGCGGGATTGGCATCTGGGAGTGGGAGTTGAAGC                                                                                                <  SRR19973065.2430281/150‑1 (MQ=60)
                                                   ACACGGATGGCACGCCGCTCTATTTTATCGCTCAGATTGAAGACATTAACGAGCTAAAACGCACCGAACAGGTCAATCAGCAACTGATGGAGCGCATAACTCTGGCTAACGAAGCGGGCGGGATTGGCATCTGGGAGTGGGAGTTGAAGC                                                                                                <  SRR19973065.657908/150‑1 (MQ=60)
                                                     ACGGATGGCACGCCGCTCTATTTTATCGCTCAGATTGAAGACATTAACGAGCTAAAACGCACCGAACAGGTCAATCAGCAACTGATGGAGCGCATAACTCTGGCTAAAGAAGCGGGCGGGATTGGCATCTGGGAGTGGGAGTTGAAGCCG                                                                                              >  SRR19973065.1497403/1‑150 (MQ=60)
                                                      CGGATGGCACGCCGCTCTATTTTATCGCTCAGATTGAAGACATTAACGAGCTAAAACGCACCGAACAGGTCAATCAGCAACTGATGGAGCGCATAACTCTGGCTAACGAAGCGGGCGGGATTGGCATCTGGGAGTGGGAGTTGAAGCCGA                                                                                             <  SRR19973065.3119474/150‑1 (MQ=60)
                                                         ATGGCACGCCGCTCTATTTTATCGCTCAGATTGAAGACATTAACGAGCTAAAACGCACCGAACAGGTCAATCAGCAACTGATGGAGCGCATAACTCTGGCTAACGAAGCGGGCGGGATTGGCATCTGGGAGTGGGAGTTGAAGCCGAATA                                                                                          <  SRR19973065.1990560/150‑1 (MQ=60)
                                                           GGCACGCCGCTCTATTTTATCGCTCAGATTGAAGACATTAACGAGCTAAAACGCACCGAACAGGTCAATCAGCAACTGATGGAGCGCATAACTCTGGCTAACGAAGCGGGCGGGATTGGCATCTGGGAGTGGGAGTTGAAGCCGAATATT                                                                                        <  SRR19973065.3376822/150‑1 (MQ=60)
                                                            GCACGCCGCTCTATTTTATCGCTCAGATTGAAGACATTAACGAGCTAAAACGCACCGAACAGGTCAATCAGCAACTGATGGAGCGCATAACTCTGGCTAACGAAGCGGGCGGGATTGGCATCTGGGAGTGGGAGTTGAAGCCGAATATTT                                                                                       <  SRR19973065.604/150‑1 (MQ=60)
                                                             CACGCCGCTCTATTTTATCGCTCAGATTGAAGACATTAACGAGCTAAAACGCACCGAACAGGTCAATCAGCAACTGATGGAGCGCATCACTCTGGCTAACGAAGCGGGCGGGATTGGCATCTGGGAGTGGGAGTTGAAGCCGAATATTTT                                                                                      >  SRR19973065.934180/1‑150 (MQ=60)
                                                                GCCGCTCTATTTTATCGCTCAGATTGAAGACATTAACGAGCTAAAACGCACCGAACAGGTCAATCAGCAACTGATGGAGCGCATAACTCTGGCTAACGAAGCGGGCGGGATTGGCATCTGGGAGTGGGAGTTGAAGCCGAATATTTTTAG                                                                                   <  SRR19973065.1562793/150‑1 (MQ=60)
                                                                GCCGCTCTATTTTATCGCTCAGATTGAAGACATTAACGAGCTAAAACGCACCGAACAGGTCAATCAGCAACTGATGGAGCGCATAACTCTGGCTAACGAAGCGGGCGGGATTGGCATCTGGGAGTGGGAGTTGAAGCCGAATATTTTTAG                                                                                   <  SRR19973065.1540270/150‑1 (MQ=60)
                                                                 CCGCTCTATTTTATCGCTCAGATTGAAGACATTAACGAGCTAAAACGCACCGAACAGGTCAATCAGCAACTGATGGAGCGCATAACTCTGGCTAACGAAGCGGGCGGGATTGGCATCTGGGAGTGGGAGTTGAAGCCGAATATTTTTAGC                                                                                  >  SRR19973065.752764/1‑150 (MQ=60)
                                                                  CGCTCTATTTTATCGCTCAGATTGAAGACATTAACGAGCTAAAACGCACCGAACAGGTCAATCAGCAACTGATGGAGCGCATAACTCTGGCTAACGAAGCGGGCGGGATTGGCATCTGGGAGTGGGAGTTGAAGCCGAATATTTTTAGCT                                                                                 <  SRR19973065.710449/150‑1 (MQ=60)
                                                                   GCTCTATTTTATCGCTCAGATTGAAGACATTAACGAGCTAAAACGCACCGAACAGGTCAATCAGCAACTGATGGAGCGCATAACTCTGGCTAACGAAGCGGGCGGGATTGGCATCTGGGAGTGGGAGTTGAAGCCGAATATTTTTAGCTG                                                                                >  SRR19973065.2927012/1‑150 (MQ=60)
                                                                    CTCTATTTTATCGCTCAGATTGAAGACATTAACGAGCTAAAACGCACCGAACAGGTCAATCAGCAACTGATGGAGCGCATAACTCTGGCTAACGAAGCGGGCGGGATTGGCATCTGGGAGTGGGAGTTGAAGCCGAATATTTTTAGCTGG                                                                               <  SRR19973065.524676/150‑1 (MQ=60)
                                                                    CTCTATTTTATCGCTCAGATTGAAGACATTAACGAGCTAAAACGCACCGAACAGGTCAATCAGCAACTGATGGAGCGCATAACTCTGGCTAACGAAGCGGGCGGGATTGGCATCTGGGAGTGGGAGTTGAAGCCGAATATTTTTAGCTGG                                                                               <  SRR19973065.525889/150‑1 (MQ=60)
                                                                      CTATTTTATCGCTCAGATTGAAGACATTAACGAGCTAAAACGCACCGAACAGGTCAATCAGCAACTGATGGAGCGCATAACTCTGGCTAACGAAGCGGGCGGGATTGGCATCTGGGAGTGGGAGTTGAAGCCGAATATTTTTAGCTGGGA                                                                             >  SRR19973065.709171/1‑150 (MQ=60)
                                                                                   CAGATTGAAGACATTAACGAGCTAAAACGCACCGAACAGGTCAATCAGCAACTGATGGAGCGCATAACTCTGGCTAACGAAGCGGGCGGGATTGGCATCTGGGAGTGGGAGTTGAAGCCGAATATTTTTAGCTGGGCTAAGCGGATGTTC                                                                <  SRR19973065.750055/150‑1 (MQ=60)
                                                                                     GATTGAAGACATTAACGAGCTAAAACGCACCGAACAGGTCAATCAGCAACTGATGGAGCGCATAACTCTGGCTAACGAAGCGGGCGGGATTGGCATCTGGGAGTGGGAGTTGAAGCCGAATATTTTTAGCTGGGATAAGCGGATGTTCGA                                                              >  SRR19973065.1448812/1‑150 (MQ=60)
                                                                                      ATTGAAGACATTAACGAGCTAAAACGCACCGAACAGGTCAATCAGCAACTGATGGAGCGCATAACTCTGGCTAACGAAGCGGGCGGGATTGGCATCTGGGAGTGGGAGTTGAAGCCGAATATTTTTAGCTGGGATAAGCGGATGTTCGAG                                                             >  SRR19973065.786463/1‑150 (MQ=60)
                                                                                      ATTGAAGACATTAACGAGCTAAAACGCACCGAACAGGTCAATCAGCAACTGATGGAGCGCATAACTCTGGCTAACGAAGCGGGCGGGATTGGCATCTGGGAGTGGGAGTTGAAGCCGAATATTTTTAGCTGGGATAAGCGGATGTTCGAG                                                             >  SRR19973065.2069719/1‑150 (MQ=60)
                                                                                      ATTGAAGACATTAACGAGCTAAAACGCACCGAACAGGTCAATCAGCAACTGATGGAGCGCATAACTCTGGCTAACGAAGCGGGCGGGATTGGCATCTGGGAGTGGGAGTTGAAGCCGAATATTTTTAGCTGGGATAAGCGGATGTTCGAG                                                             >  SRR19973065.786394/1‑150 (MQ=60)
                                                                                       TTGAAGACATTAACGAGCTAAAACGCACCGAACAGGTCAATCAGCAACTGATGGAGCGCATAACTCTGGCTAACGAAGCGGGCGGGATTGGCATCTGGGAGTGGGAGTTGAAGCCGAATATTTTTAGCTGGGATAAGCGGATGTTCGAGC                                                            >  SRR19973065.2479409/1‑150 (MQ=60)
                                                                                        TGAAGACATTAACGAGCTAAAACGCACCGAACAGGTCAATCAGCAACTGATGGAGCGCATAACTCTGGCTAACGAAGCGGGTGGGATTGGCATCTGGGAGTGGGAGTTGAAGCCGAATATTTTTAGCTGGGATAAGCGGATGTTCGAGCT                                                           <  SRR19973065.2636989/150‑1 (MQ=60)
                                                                                        TGAAGACATTAACGAGCTAAAACGCACCGAACAGGTCAATCAGCAACTGATGGAGCGCATAACTCTGGCTAACGAAGCGGGCGGGATTGGCATCTGGGAGTGGGAGTTGAAGCCGAATATTTTTAGCTGGGATAAGCGGATGTTCGAGCT                                                           >  SRR19973065.337755/1‑150 (MQ=60)
                                                                                         GAAGACATTAACGAGCTAAAACGCACCGAACAGGTCAATCAGCAACTGATGGAGCGCATAACTCTGGCTAACGAAGCGGGCGGGATTGGCATCTGGGAGTGGGAGTTGAAGCCGAATATTTTTAGCTGGGATAAGCGGATGTTCGAGCTG                                                          <  SRR19973065.2895597/150‑1 (MQ=60)
                                                                                           AGACATTAACGAGCTAAAACGCACCGAACAGGTCAATCAGCAACTGATGGAGCGCATAACTCTGGCTAACGAAGCGGGCGGGATTGGCATCTGGGAGTGGGAGTTGAAGCCGAATATTTTTAGCTGGGATAAGCGGATGTTCGAGCTGTA                                                        >  SRR19973065.5863/1‑150 (MQ=60)
                                                                                               ATTAACGAGCTAAAACGCACCGAACAGGTCAATCAGCAACTGATGGAGCGCATAACTCTGGCTAACGAAGCGGGCGGGATTGGCATCTGGGAGTGGGAGTTGAAGCCGAATATTTTTAGCTGGGATAAGCGGATGTTCGAGCTGTATGAA                                                    >  SRR19973065.998506/1‑150 (MQ=60)
                                                                                               ATTAACGAGCTAAAACGCACCGAACAGGTCAATCAGCAACTGATGGAGCGCATAACTCTGGCTAACGAAGCGGGCGGGATTGGCATCTGGGAGTGGGAGTTGAAGCCGAATATTTTTAGCTGGGATAAGCGGATGTTCGAGCTGTATGAA                                                    >  SRR19973065.217385/1‑150 (MQ=60)
                                                                                               ATTAACGAGCTAAAACGCACCGAACAGGTCAATCAGCAACTGATGGAGCGCATAACTCTGGCTAACGAAGCGGGCGGGATTGGCATCTGGGAGTGGGAGTTGAAGCCGAATATTTTTAGCTGGGATAAGCGGATGTTCGAGCTGTATGAA                                                    >  SRR19973065.2762965/1‑150 (MQ=60)
                                                                                                  AACGAGCTAAAACGCACCGAACAGGTCAATCAGCAACTGATGGAGCGCATAACTCTGGCTAACGAAGCGGGCGGGATTGGCATCTGGGAGTGGGAGTTGAAGCCGAATATTTTTAGCTGGGATAAGCGGATGTTCGAGCTGTATGAAATT                                                 <  SRR19973065.994673/150‑1 (MQ=60)
                                                                                                  AACGAGCTAAAACGCACCGAACAGGTCAATCAGCAACTGATGGAGCGCATAACTCTGGCTAACGAAGCGGGCGGGATTGGCATCTGGGAGTGGGAGTTGAAGCCGAATATTTTTAGCTGGGATAAGCGGATGTTCGAGCTGTATGAAATT                                                 >  SRR19973065.2790873/1‑150 (MQ=60)
                                                                                                   ACGAGCTAAAACGCACCGACCAGGTCCCTCAGCAACTGAGGGAGCGCATAACTCTGGCTAACGAAGCGGGCGGGATTGGCATCTGGGAGTGGGAGTTGAAGCCGAATATTTTTAGCTGGGATAAGCGGATGTTCGAGCTGTATGAAATTC                                                <  SRR19973065.2689308/150‑1 (MQ=60)
                                                                                                       GCTAAAACGCACCGAACAGGTCAATCAGCAACTGATGGAGCGCATAACTCTGGCTAACGAAGCGGGCGGGATTGGCATCTGGGAGTGGGAGTTGAAGCCGAATATTTTTAGCTGGGATAAGCGGATGTTCGAGCTGTATGAAATTCCTCC                                            <  SRR19973065.2155609/150‑1 (MQ=60)
                                                                                                        CTAAAACGCACCGAACAGGTCAATCAGCAACTGATGGAGCGCATAACTCTGGCTAACGAAGCGGGCGGGATTGGCATCTGGGAGTGGGAGTTGAAGCCGAATATTTTTAGCTGGGATAAGCGGATGTTCGAGCTGTATGAAATTCCTCCA                                           <  SRR19973065.985257/150‑1 (MQ=60)
                                                                                                        CTAAAACGCACCGAACAGGTCAATCAGCAACTGATGGAGCGCATAACTCTGGCTAACGAAGCGGGCGGGATTGGCATCTGGGAGTGGGAGTTGAAGCCGAATATTTTTAGCTGGGATAAGCGGATGTTCGAGCTGTATGAAATTCCTCCA                                           <  SRR19973065.985104/150‑1 (MQ=60)
                                                                                                           AAACGCACCGAACAGGTCAATCAGCAACTGATGGAGCGCATAACTCTGGCTAACGAAGCGGGCGGGATTGGCATCTGGGAGTGGGAGTTGAAGCCGAATATTTTTAGCTGGGATAAGCGGATGTTCGAGCTGTATGAAATTCCTCCACAT                                        <  SRR19973065.2474397/150‑1 (MQ=60)
                                                                                                           AAACGCACCGAACAGGTCAATCAGCAACTGATGGAGCGCATAACTCTGGCTAACGAAGCGGGCGGGATTGGCATCTGGGAGTGGGAGTTGAAGCCGAATATTTTTAGCTGGGATAAGCGGATGTTCGAGCTGTATGAAATTCCTCCACAT                                        >  SRR19973065.1584620/1‑150 (MQ=60)
                                                                                                           AAACGCACCGAACAGGTCAATCAGCAACTGATGGAGCGCATAACTCTGGCTAACGAAGCGGGCGGGATTGGCATCTGGGAGTGGGAGTTGAAGCCGAATATTTTTAGCTGGGATAAGCGGATGTTCGAGCTGTATGAAATTCCTCCACAT                                        <  SRR19973065.1142179/150‑1 (MQ=60)
                                                                                                              CGCCCCGAACAGGTCAATCAGCAACTGATGGAGCGCATAACTCTGGCTAACGAAGCGGGCGGGATTGGCATCTGGGAGTGGGAGTTGAAGCCGAATATTTTTAGCTGGGATAAGCGGATGTTCGAGCTGTATGAAATTCCTCCACATATC                                     >  SRR19973065.877004/1‑150 (MQ=60)
                                                                                                              CGCACCGAACAGGTCAATCAGCAACTGATGGAGCGCATAACTCTGGCTAACGAAGCGGGCGGGATTGGCATCTGGGAGTGGGAGTTGAAGCCGAATATTTTTAGCTGGGATAAGCGGATGTTCGAGCTGTATGAAATTCCTCCACATATC                                     >  SRR19973065.1009331/1‑150 (MQ=60)
                                                                                                               GCACCGAACAGGTCAATCAGCAACTGATGGAGCGCATAACTCTGGCTAACGAAGCGGGCGGGATTGGCATCTGGGAGTGGGAGTTGAAGCCGAATATTTTTAGCTGGGATAAGCGGATGTTCGAGCTGTATGAAATTCCTCCACATATCA                                    <  SRR19973065.3365504/150‑1 (MQ=60)
                                                                                                                CACCGAACAGGTCAATCAGCAACTGATGGAGCGCATAACTCTGGCTAACGAAGCGGGCGGGATTGGCATCTGGGAGTGGGAGTTGAAGCCGAATATTTTTAGCTGGGATAAGCGGATGTTCGAGCTGTATGAAATTCCTCCACATATCAA                                   >  SRR19973065.942780/1‑150 (MQ=60)
                                                                                                                     AACAGGTCAATCAGCAACTGATGGAGCGCATAACTCTGGCTAACGAAGCGGGCGGGATTGGCATCTGGGAGTGGGAGTTGAAGCCGAATATTTTTAGCTGGGATAAGCGGATGTTCGAGCTGTATGAAATTCCTCCACATATCAAACCGA                              >  SRR19973065.472021/1‑150 (MQ=60)
                                                                                                                      ACAGGTCAATCAGCAACTGATGGAGCGCATAACTCTGGCTAACGAAGCGGGCGGGATTGGCATCTGGGAGTGGGAGTTGAAGCCGAATATTTTTAGCTGGGATAAGCGGATGTTCGAGCTGTATGAAATTCCTCCACATATCAAACCGAA                             >  SRR19973065.2889344/1‑150 (MQ=60)
                                                                                                                          GTCAATCAGCAACTGATGGAGCGCATAACTCTGGCTAACGAAGCGGGCGGGATTGGCATCTGGGAGTGGGAGTTGAAGCCGAATATTTTTAGCTGGGATAAGCGGATGTTCGAGCTGTATGAAATTCCTCCACATATCAAACCGAACTGG                         <  SRR19973065.561247/150‑1 (MQ=60)
                                                                                                                          GTCAATCAGCAACTGATGGAGCGCATAACTCTGGCTAACGAAGCGGGCGGGATTGGCATCTGGGAGTGGGAGTTGAAGCCGAATATTTTTAGCTGGGATAAGCGGATGTTCGAGCTGTATGAAATTCCTCCACATATCAAACCGAACTGG                         <  SRR19973065.432027/150‑1 (MQ=60)
                                                                                                                          GTCAATCAGCAACTGATGGAGCGCATAACTCTGGCTAACGAAGCGGGCGGGATTGGCATCTGGGAGTGGGAGTTGAAGCCGAATATTTTTAGCTGGGATAAGCGGATGTTCGAGCTGTATGAAATTCCTCCACATATCAAACCGAACTGG                         <  SRR19973065.432109/150‑1 (MQ=60)
                                                                                                                              ATCAGCAACTGATGGAGCGCATAACTCTGGCTAACGAAGCGGGCGGGATTGGCATCTGGGAGTGGGAGTTGAAGCCGAATATTTTTAGCTGGGATAAGCGGATGTTCGAGCTGTATGAAATTCCTCCACATATCAAACCGAACTGGCAGG                     <  SRR19973065.2757580/150‑1 (MQ=60)
                                                                                                                                 AGCAACTGATGGAGCGCATAACTCTGGCTAACGAAGCGGGCGGGATTGGCATCTGGGAGTGGGAGTTGAAGCCGAATATTTTTAGCTGGGATAAGCGGATGTTCGAGCTGTATGAAATTCCTCCACATATCAAACCGAACTGGCAGGTGT                  >  SRR19973065.2665805/1‑150 (MQ=60)
                                                                                                                                 AGCAACTGATGGAGCGCATAACTCTGGCTAACGAAGCGGGCGGGATTGGCATCTGGGAGTGGGAGTTGAAGCCGAATATTTTTAGCTGGGATAAGCGGATGTTCGAGCTGTATGAAATTCCTCCACATATCAAACCGAACTGGCAGGTGT                  >  SRR19973065.1018884/1‑150 (MQ=60)
                                                                                                                                 AGCAACTGATGGAGCGCATAACTCTGGCTAACGAAGCGGGCGGGATTGGCATCTGGGAGTGGGAGTTGAAGCCGAATATTTTTAGCTGGGATAAGCGGATGTTCGAGCTGTATGAAATTCCTCCACATATCAAACCGAACTGGCAGGTGT                  >  SRR19973065.923334/1‑150 (MQ=60)
                                                                                                                                 AGCAACTGATGGAGCGCATAACTCTGGCTAACGAAGCGGGCGGGATTGGCATCTGGGAGTGGGAGTTGAAGCCGAATATTTTTAGCTGGGATAAGCGGATGTTCGAGCTGTATGAAATTCCTCCACATATCAAACCGAACTGGCAGGTGT                  >  SRR19973065.2665852/1‑150 (MQ=60)
                                                                                                                                 AGCAACTGATGGAGCGCATAACTCTGGCTAACGAAGCGGGCGGGATTGGCATCTGGGAGTGGGAGTTGAAGCCGAATATTTTTAGCTGGGATAAGCGGATGTTCGAGCTGTATGAAATTCCTCCACATATCAAACCGAACTGGCAGGTGT                  >  SRR19973065.2665769/1‑150 (MQ=60)
                                                                                                                                  GCATCTGATGGAGCGCATAACTCTGGCTAACGAAGCGGGCGGGATTGGCATCTGGGAGTGGGAGTTGAAGCCGAATATTTTTAGCTGGGATAAGCGGATGTTCGAGCTGTATGAAATTCCTCCACATATCAAACCGAACTGGCAGGTGTG                 <  SRR19973065.3346766/150‑1 (MQ=60)
                                                                                                                                  GCAACTGATGGAGCGCATAACTCTGGCTAACGAAGCGGGCGGGATTGGCATCTGGGAGTGGGAGTTGAAGCCGAATATTTTTAGCTGGGATAAGCGGATGTTCGAGCTGTATGAAATTCCTCCACATATCAAACCGAACTGGCAGGTGTG                 <  SRR19973065.3462228/150‑1 (MQ=60)
                                                                                                                                  GCAACTGATGGAGCGCATAACTCTGGCTAACGAAGCGGGCGGGATTGGCATCTGGGAGTGGGAGTTGAAGCCGAATATTTTTAGCTGGGATAAGCGGATGTTCGAGCTGTATGAAATTCCTCCACATATCAAACCGAACTGGCAGGTGTG                 <  SRR19973065.3346741/150‑1 (MQ=60)
                                                                                                                                    AACTGATGGAGCGCATAACTCTGGCTAACGAAGCGGGCGGGATTGGCATCTGGGAGTGGGAGTTGAAGCCGAATATTTTTAGCTGGGATAAGCGGATGTTCGAGCTGTATGAAATTCCTCCACATATCAAACCGAACTGGCAGGTGTGGT               >  SRR19973065.1873041/1‑150 (MQ=60)
                                                                                                                                       TGATGGAGCGCATAACTCTGGCTAACGAAGCGGGCGGGATTGGCATCTGGGAGTGGGAGTTGAAGCCGAATATTTTTAGCTGGGATAAGCGGATGTTCGAGCTGTATGAAATTCCTCCACATATCAAACCGAACTGGCAGGTGTGGTACG            >  SRR19973065.1135173/1‑150 (MQ=60)
                                                                                                                                       TGATGGAGCGCATAACTCTGGCTAACGAAGCGGGCGGGATTGGCATCTGGGAGTGGGAGTTGAAGCCGAATATTTTTAGCTGGGATAAGCGGATGTTCGAGCTGTATGAAATTCCTCCACATATCAAACCGAACTGGCAGGTGTGGTACG            >  SRR19973065.1135327/1‑150 (MQ=60)
                                                                                                                                          TGGAGCGCATAACTCTGGCTAACGAAGCGGGCGGGATTGGCATCTGGGAGTGGGAGTTGAAGCCGAATATTTTTAGCTGGGATAAGCGGATGTTCGAGCTGTATGAAATTCCTCCACATATCAAACCGAACTGGCAGGTGTGGTACGAGT         <  SRR19973065.247311/150‑1 (MQ=60)
                                                                                                                                          TGGAGCGCATAACTCTGGCTAACGAAGCGGGCGGGATTGGCATCTGGGAGTGGGAGTTGAAGCCGAATATTTTTAGCTGGGATAAGCGGATGTTCGAGCTGTATGAAATTCCTCCACATATCAAACCGAACTGGCAGGTGTGGTACGAGT         <  SRR19973065.247356/150‑1 (MQ=60)
                                                                                                                                            GAGCGCATAACTCTGGCTAACGAAGCGGGCGGGATTGGCATCTGGGAGTGGGAGTTGAAGCCGAATATTTTTAGCTGGGATAAGCGGATGTTCGAGCTGTATGAAATTCCTCCACATATCAAACCGAACTGGCAGGTGTGGTACGAGTGC       <  SRR19973065.1661116/150‑1 (MQ=60)
                                                                                                                                               CGCATAACTCTGGCTAACGAAGCGGGCGGGATTGGCATCTGGGTGTGGGAGTTGAAGCCGAATATTTTTAGCTGGGATAAGCGGATGTTCGAGCTGTATGAAATTCCTCCACATATCAAACCGAACTGGCAGGTGTGGTACGAGTGCGTG    <  SRR19973065.1929196/150‑1 (MQ=60)
                                                                                                                                               CGCATAACTCTGGCTAACGAAGCGGGCGGGATTGGCATCTGGGAGTGGGAGTTGAAGCCGAATATTTTTAGCTGGGATAAGCGGATGTTCGAGCTGTATGAAATTCCTCCACATATCAAACCGAACTGGCAGGTGTGGTACGAGTGCGTG    >  SRR19973065.2326226/1‑150 (MQ=60)
                                                                                                                                               CGCATAACTCTGGCTAACGAAGCGGGCGGGATTGGCATCTGGGAGTGGGAGTTGAAGCCGAATATTTTTAGCTGGGATAAGCGGATGTTCGAGCTGTATGAAATTCCTCCACATATCAAACCGAACTGGCAGGTGTGGTACGAGTGCGTG    <  SRR19973065.3320078/150‑1 (MQ=60)
                                                                                                                                               CGCATAACTCTGGCTAACGAAGCGGGCGGGATTGGCATCTGGGAGTGGGAGTTGAAGCCGAATATTTTTAGCTGGGATAAGCGGATGTTCGAGCTGTATGAAATTCCTCCACATATCAAACCGAACTGGCAGGTGTGGTACGAGTGCGTG    <  SRR19973065.1929373/150‑1 (MQ=60)
                                                                                                                                                 CATAACTCTGGCTAACGAAGCGGGCGGGATTGGCATCTGGGAGTGGGAGTTGAAGCCGAATATTTTTAGCTGGGATAAGCGGATGTTCGAGCTGTATGAAATTCCTCCACATATCAAACCGAACTGGCAGGTGTGGTACGAGTGCGTGCT  <  SRR19973065.3494638/150‑1 (MQ=60)
                                                                                                                                                 CATAACTCTGGCTAACGAAGCGGGCGGGATTGGCATCTGGGAGTGGGAGTTGAAGCCGAATATTTTTAGCTGGGATAAGCGGATGTTCGAGCTGTATGAAATTCCTCCACATATCAAACCGAACTGGCAGGTGTGGTACGAGTGCGTGCT  <  SRR19973065.3054500/150‑1 (MQ=60)
                                                                                                                                                 CATAACTCTGGCTAACGAAGCGGGCGGGATTGGCATCTGGGAGTGGGAGTTGAAGCCGAATATTTTTAGCTGGGATAAGCGGATGTTCGAGCTGTATGAAATTCCTCCACATATCAAACCGAACTGGCAGGTGTGGTACGAGTGCGTGCT  <  SRR19973065.3096362/150‑1 (MQ=60)
                                                                                                                                                    |                                                                                                                                                  
ACCGCAATGGCGATGTTGTCTGGGCGTTGCTTGCCGTCTCACTGGTGCGCCACACGGATGGCACGCCGCTCTATTTTATCGCTCAGATTGAAGACATTAACGAGCTAAAACGCACCGAACAGGTCAATCAGCAACTGATGGAGCGCATCACTCTGGCTAACGAAGCGGGCGGGATTGGCATCTGGGAGTGGGAGTTGAAGCCGAATATTTTTAGCTGGGATAAGCGGATGTTCGAGCTGTATGAAATTCCTCCACATATCAAACCGAACTGGCAGGTGTGGTACGAGTGCGTGCT  >  CP009273/2137891‑2138185

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑