Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F434 I0 R1 1130 215.9 7031662 97.1% 6827743 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,243,991 T→A 100% W266R (TGG→AGG)  yeiH → UPF0324 family inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,243,9910TA98.6% 703.2 / ‑0.4 213W266R (TGG→AGG) yeiHUPF0324 family inner membrane protein
Reads supporting (aligned to +/- strand):  ref base T (1/2);  new base A (107/103);  total (108/105)
Fisher's exact test for biased strand distribution p-value = 6.18e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.91e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 213 total aligned reads displayed.

ATCAGCCCGGATGCGGAAAACGCAGCAGTTATTTCCAAAATGCTGCGCGTGATGATGCTGGCTCCGTTCCTCATCCTGCTGGCGGCGCGTGTTAAACAGCTGTCTGGGGCGAACAGCGGCGAGAAAAGCAAAATCACTATTCCGTGGTTTGCCATCTTGTTCATCGTAGTTGCCATCTTTAACTCGTTCCACCTGTTACCGCAGAGCGTGGTGAACATGCTGGTAACGCTGGATACCTTCCTGCTGGCAATGGCGATGGCGGCGCTGGGTCTGACCACTCACGTCAGCG  >  CP009273/2243847‑2244135
                                                                                                                                                |                                                                                                                                                
aTCAGCCCGGATGCTGAAAACGCAGCAGTTATTTCCAAAATGCTGCGCGTGATGATGCTGGCTCCGTTCCTCATCCTGCTGGCGGCGCGTGTTAAACAGCTGTCTGGGGCGAACAGCGGCGAGAAAAGCAAAATCACTATTCCGAGGt                                                                                                                                               <  2:2457736/148‑1 (MQ=255)
  cAGCCCGGATGCGGAAAACGCAGCAGTTATTTCCAAAATGCTGCGCGTGATGATGCTGGCTCCGTTCCTCATCCTGCTGGCGGCGCGTGTTAAACAGCTGTCTGGGGCGAACAGCGGCGAGAAAAGCAAAATCACTATTCCGAGGttt                                                                                                                                             >  1:2758774/1‑148 (MQ=255)
   aGCCCGGATGCGGAAAACGCAGCAGTTATTTCCAAAATGCTGCGCGTGATGATGCTGGCTCCGTTCCTCATCCTGCTGGCGGCGCGTGTTAAACAGCTGTCTGGGGCGAACAGCGGCGAGAAAAGCAAAATCACTATTCCGAGGTTTg                                                                                                                                            <  1:2493673/148‑1 (MQ=255)
     cccGGATGCGGAAAACGCAGCAGTTATTTCCAAAATGCTGCGCGTGATGATGCTGGCTCCGTTCCTCATCCTGCTGGCGGCGCGTGTTAAACAGCTGTCTGGGGCGAACAGCGGCGAGAAAAGCAAAATCACTATTCCGAGGTTTGcc                                                                                                                                          >  2:1488362/1‑148 (MQ=255)
     cccGGATGCGGAAAACGCAGCAGTTATTTCCAAAATGCTGCGCGTGATGATGCTGGCTCCGTTCCTCATCCTGCTGGCGGCGCGTGTTAAACAGCTGTCTGGGGCGAACAGCGGCGAGAAAAGCAAAATCACTATTCCGAGGTTTGcc                                                                                                                                          >  2:2946553/1‑148 (MQ=255)
       cGGATGCGGAAAACGCAGCAGTTATTTCCAAAATGCTGCGCGTGATGATGCTGGCTCCGTTCCTCATCCTGCTGGCGGCGCGTGTTAAACAGCTGTCTGGGGCGAACAGCGGCGAGAAAAGCAAAATCACTATTCCGAGGTTTGCCAt                                                                                                                                        >  1:3301705/1‑148 (MQ=255)
       cGGATGCGGAAAACGCAGCAGTTATTTCCAAAATGCTGCGCGTGATGATGCTGGCTCCGTTCCTCATCCTGCTGGCGGCGCGTGTTAAACAGCTGTCTGGGGCGAACAGCGGCGAGAAAAGCAAAATCACTATTCCGAGGTTTGCCAt                                                                                                                                        >  1:244522/1‑148 (MQ=255)
       cGGATGCGGAAAACGCAGCAGTTATTTCCAAAATGCTGCGCGTGATGATGCTGGCTCCGTTCCTCATCCTGCTGGCGGCGCGTGTTAAACAGCTGTCTGGGGCGAACAGCGGCGAGAAAAGCAAAATCACTATTCCGAGGTTTGCCAt                                                                                                                                        >  1:2017847/1‑148 (MQ=255)
        ggATGCGGAAAACGCAGCAGTTATTTCCAAAATGCTGCGCGTGATGATGCTGGCTCCGTTCCTCATCCTGCTGGCGGCGCGTGTTAAACAGCTGTCTGGGGCGAACAGCGGCGAGAAAAGCAAAATCACTATTCCGAGGTTTGCCATc                                                                                                                                       <  1:949499/148‑1 (MQ=255)
          aTGCGGAAAACGCAGCAGTTATTTCCAAAATGCTGCGCGTGATGATGCTGGCTCCGTTCCTCATCCTGCTGGCGGCGCGTGTTAAACAGCTGTCTGGGGCGAACAGCGGCGAGAAAAGCAAAATCACTATTCCGAGGTTTGCCATCtt                                                                                                                                     >  1:1138362/1‑148 (MQ=255)
          aTGCGGAAAACGCAGCAGTTATTTCCAAAATGCTGCGCGTGATGATGCTGGCTCCGTTCCTCATCCTGCTGGCGGCGCGTGTTAAACAGCTGTCTGGGGCGAACAGCGGCGAGAAAAGCAAAATCACTATTCCGAGGTTTGCCATCtt                                                                                                                                     >  2:127090/1‑148 (MQ=255)
           tGCGGAAAACGCAGCAGTTATTTCCAAAATGCTGCGCGTGATGATGCTGGCTCCGTTCCTCATCCTGCTGGCGGCGCGTGTTAAACAGCTGTCTGGGGCGAACAGCGGCGAGAAAAGCAAAATCACTATTCCGAGGTTTGCCATCTTg                                                                                                                                    >  1:2117197/1‑148 (MQ=255)
               gAAAACGCAGCAGTTATTTCCAAAATGCTGCGCGTGATGATGCTGGCTCCGTTCCTCATCCTGCTGGCGGCGCGTGTTAAACAGCTGTCTGGGGCGAACAGCGGCGAGAAAAGCAAAATCACTATTCCGTGGTTTGCCATCTTGTTCa                                                                                                                                <  1:1814055/148‑1 (MQ=255)
               gAAAACGCAGCAGTTATTTCCAAAATGCTGCGCGTGATGATGCTGGCTCCGTTCCTCATCCTGCTGGCGGCGCGTGTTAAACAGCTGTCTGGGGCGAACAGCGGCGAGAAAAGCAAAATCACTATTCCGAGGTTTGCCATCTTGTTCa                                                                                                                                <  2:2562228/148‑1 (MQ=255)
               gAAAACGCAGCAGTTATTTCCAAAATGCTGCGCGTGATGATGCTGGCTCCGTTCCTCATCCTGCTGGCGGCGCGTGTTAAACAGCTGTCTGGGGCGAACAGCGGCGAGAAAAGCAAAATCACTATTCCGAGGTTTGCCATCTTGTTCa                                                                                                                                <  2:2382039/148‑1 (MQ=255)
                   aCGCAGCAGTTATTTCCAAAATGCTGCGCGTGATGATGCTGGCTCCGTTCCTCATCCTGCTGGCGGCGCGTGTTAAACAGCTGTCTGGGGCGAACAGCGGCGAGAAAAGCAAAATCACTATTCCGAGGTTTGCCATCTTGTTCATCGt                                                                                                                            >  2:867863/1‑148 (MQ=255)
                      cagcagTTATTTCCAAAATGCTGCGCGTGATGATGCTGGCTCCGTTCCTCATCCTGCTGGCGGCGCGTGTTAAACAGCTGTCTGGGGCGAACAGCGGCGAGAAAAGCAAAATCACTATTCCGAGGTTTGCCATCTTGTTCATCGTAGt                                                                                                                         <  1:2930796/148‑1 (MQ=255)
                      cagcagTTATTTCCAAAATGCTGCGCGTGATGATGCTGGCTCCGTTCCTCATCCTGCTGGCGGCGCGTGTTAAACAGCTGTCTGGGGCGAACAGCGGCGAGAAAAGCAAAATCACTATTCCGAGGTTTGCCATCTTGTTCATCGTAGt                                                                                                                         <  1:3427934/148‑1 (MQ=255)
                      cagcagTTATTTCCAAAATGCTGCGCGTGATGATGCTGGCTCCGTTCCTCATCCTGCTGGCGGCGCGTGTTAAACAGCTGTCTGGGGCGAACAGCGGCGAGAAAAGCAAAATCACTATTCCGAGGTTTGCCATCTTGTTCATCGTAGt                                                                                                                         <  1:2695371/148‑1 (MQ=255)
                         cagTTATTTCCAAAATGCTGCGCGTGATGATGCTGGCTCCGTTCCTCATCCTGCTGGCGGCGCGTGTTAAACAGCTGTCTGGGGCGAACAGCGGCGAGAAAAGCAAAATCACTATTCCGAGGTTTGCCATCTTGTTCATCGTAGTTGc                                                                                                                      <  2:289258/148‑1 (MQ=255)
                          agTTATTTCCAAAATGCTGCGCGTGATGATGCTGGCTCCGTTCCTCATCCTGCTGGCGGCGCGTGTTAAACAGCTGTCTGGGGCGAACAGCGGCGAGAAAAGCAAAATCACTATTCCGAGGTTTGCCATCTTGTTCATCGTAGTTGcc                                                                                                                     <  2:2175500/148‑1 (MQ=255)
                            ttATTTCCAAAATGCTGCGCGTGATGTTGCTGGCTCCGTTCCTCATCCTGCTGGCGGCGCGTGTTCAACAGCTGTCTGGGGCGAACAGCGGCGAGAAAAGCCAAATCACTATTCCGAGGTTTGCCATCTTGTTCATCGTAGTTGCCAt                                                                                                                   >  2:724789/1‑148 (MQ=255)
                            ttATTTCCAAAATGCTGCGCGTGATGATGCTGGCTCCGTTCCTCATCCTGCTGGCGGCGCGTGTTAAACAGCTGTCTGGGGCGAACAGCGGCGAGAAAAGCAAAATCACTATTCCGAGGTTTGCCATCTTGTTCATCGTAGTTGCCAt                                                                                                                   <  2:252365/148‑1 (MQ=255)
                             tATTTCCAAAATGCTGCGCGTGATGATGCTGGCTCCGTTCCTCATCCTGCTGGCGGCGCGTGTTAAACAGCTGTCTGGGGCGAACAGCGGCGAGAAAAGCAAAATCACTATTCCGAGGTTTGCCATCTTGTTCATCGTAGTTGCCATc                                                                                                                  <  1:1697046/148‑1 (MQ=255)
                              aTTTCCAAAATGCTGCGCGTGATGATGCTGGCTCCGTTCCTCATCCTGCTGGCGGCGCGTGTTAAACAGCTGTCTGGGGCGAACAGCGGCGAGAAAAGCAAAATCACTATTCCGAGGTTTGCCATCTTGTTCATCGTAGTTGCCATCt                                                                                                                 <  2:1497916/148‑1 (MQ=255)
                              aTTTCCAAAATGCTGCGCGTGATGATGCTGGCTCCGTTCCTCATCCTGCTGGCGGCGCGTGTTAAACAGCTGTCTGGGGCGAACAGCGGCGAGAAAAGCAAAATCACTATTCCGAGGTTTGCCATCTTGTTCATCGTAGTTGCCATCt                                                                                                                 <  2:2445849/148‑1 (MQ=255)
                                ttCCAAAATGCTGCGCGTGATGATGCTGGCTCCGTTCCTCATCCTGCTGGCGGCGCGTGTTAAACAGCTGTCTGGGGCGAACAGCGGCGAGAAAAGCAAAATCACTATTCCGAGGTTTGCCATCTTGTTCATCGTAGTTGCCATCttt                                                                                                               <  2:2937887/148‑1 (MQ=255)
                                     aaaTGCTGCGCGTGATGATGCTGGCTCCGTTCCTCATCCTGCTGGCGGCGCGTGTTAAACAGCTGTCTGGGGCGAACAGCGGCGAGAAAAGCAAAATCACTATTCCGAGGTTTGCCATCTTGTTCATCGTAGTTGCCATCTTTAACTc                                                                                                          >  1:2769583/1‑148 (MQ=255)
                                      aaTGCTGCGCGTGATGATGCTGGCTCCGTTCCTCATCCTGCTGGCGGCGCGTGTTAAACAGCTGTCTGGGGCGAACAGCGGCGAGAAAAGCAAAATCACTATTCCGAGGTTTGCCATCTTGTTCATCGTAGTTGCCATCTTTAACTCg                                                                                                         <  2:467630/148‑1 (MQ=255)
                                      aaTGCTGCGCGTGATGATGCTGGCTCCGTTCCTCATCCTGCTGGCGGCGCGTGTTAAACAGCTGTCTGGGGCGAACAGCGGCGAGAAAAGCAAAATCACTATTCCGAGGTTTGCCATCTTGTTCATCGTAGTTGCCATCTTTAACTCg                                                                                                         <  2:468215/148‑1 (MQ=255)
                                      aaTGCTGCGCGTGATGATGCTGGCTCCGTTCCTCATCCTGCTGGCGGCGCGTGTTAAACAGCTGTCTGGGGCGAACAGCGGCGAGAAAAGCAAAATCACTATTCCGAGGTTTGCCATCTTGTTCATCGTAGTTGCCATCTTTAACTCg                                                                                                         >  2:3492750/1‑148 (MQ=255)
                                        tgctgcGCGTGATGATGCTGGCTCCGTTCCTCATCCTGCTGGCGGCGCGTGTTAAACAGCTGTCTGGGGCGAACAGCGGCGAGAAAAGCAAAATCACTATTCCGAGGTTTGCCATCTTGTTCATCGTAGTTGCCATCTTTAACTCGtt                                                                                                       >  1:2644757/1‑148 (MQ=255)
                                         gctgcGCGTGATGATGCTGGCTCCGTTCCTCATCCTGCTGGCGGCGCGTGTTAAACAGCTGTCTGGGGCGAACAGCGGCGAGAAAAGCAAAATCACTATTCCGAGGTTTGCCATCTTGTTCATCGTAGTTGCCATCTTTAACTCGTTc                                                                                                      <  1:295862/148‑1 (MQ=255)
                                         gctgcGCGTGATGATGCTGGCTCCGTTCCTCATCCTGCTGGCGGCGCGTGTTAAACAGCTGTCTGGGGCGAACAGCGGCGAGAAAAGCAAAATCACTATTCCGAGGTTTGCCATCTTGTTCATCGTAGTTGCCATCTTTAACTCGTTc                                                                                                      <  1:3113641/148‑1 (MQ=255)
                                            gcgcGTGATGATGCTGGCTCCGTTCCTCATCCTGCTGGCGGCGCGTGTTAAACAGCTGTCTGGGGCGAACAGCGGCGAGAAAAGCAAAATCACTATTCCGAGGTTTGCCATCTTGTTCATCGTAGTTGCCATCTTTAACTCGTTCCAc                                                                                                   >  2:1057540/1‑148 (MQ=255)
                                             cgcgTGATGATGCTGGCTCCGTTCCTCATCCTGCTGGCGGCGCGTGTTAAACAGCTGTCTGGGGCGAACAGCGGCGAGAAAAGCAAAATCACTATTCCGAGGTTTGCCATCTTGTTCATCGTAGTTGCCATCTTTAACTCGTTCCAcc                                                                                                  <  2:2890915/148‑1 (MQ=255)
                                             cgcgTGATGATGCTGGCTCCGTTCCTCATCCTGCTGGCGGCGCGTGTTAAACAGCTGTCTGGGGCGAACAGCGGCGAGAAAAGCAAAATCACTATTCCGAGGTTTGCCATCTTGTTCATCGTAGTTGCCATCTTTAACTCGTTCCAcc                                                                                                  <  1:1739361/148‑1 (MQ=255)
                                             cgcgTGATGATGCTGGCTCCGTTCCTCATCCTGCTGGCGGCGCGTGTTAAACAGCTGTCTGGGGCGAACAGCGGCGAGAAAAGCAAAATCACTATTCCGAGGTTTGCCATCTTGTTCATCGTAGTTGCCATCTTTAACTCGTTCCAcc                                                                                                  >  1:2820931/1‑148 (MQ=255)
                                                 tgatgaTGCTGGCTCCGTTCCTCATCCTGCTGGCGGCGCGTGTTAAACAGCTGTCTGGGGCGAACAGCGGCGAGAAAAGCAAAATCACTATTCCGAGGTTTGCCATCTTGTTCATCGTAGTTGCCATCTTTAACTCGTTCCACCTGtt                                                                                              >  1:2993466/1‑148 (MQ=255)
                                                 tgatgaTGCTGGCTCCGTTCCTCATCCTGCTGGCGGCGCGTGTTAAACAGCTGTCTGGGGCGAACAGCGGCGAGAAAAGCAAAATCACTATTCCGAGGTTTGCCATCTTGTTCATCGTAGTTGCCATCTTTAACTCGTTCCACCTGtt                                                                                              >  1:1240137/1‑148 (MQ=255)
                                                  gatgatGCTGGCTCCGTTCCTCATCCTGCTGGCGGCGCGTGTTAAACAGCTGTCTGGGGCGAACAGCGGCGAGAAAAGCAAAATCACTATTCCGAGGTTTGCCATCTTGTTCATCGTAGTTGCCATCTTTAACTCGTTCCACCTGTTa                                                                                             <  2:2925715/148‑1 (MQ=255)
                                                      atgCTGGCTCCGTTCCTCATCCTGCTGGCGGCGCGTGTTAAACAGCTGTCTGGGGCGAACAGCGGCGAGAAAAGCAAAATCACTATTCCGAGGTTTGCCATCTTGTTCATCGTAGTTGCCATCTTTAACTCGTTCCACCTGTTACCGc                                                                                         <  2:577368/148‑1 (MQ=255)
                                                          tGGCTCCGTTCCTCATCCTGCTGGCGGCGCGTGTTAAACAGCTGTCTGGGGCGAACAGCGGCGAGAAAAGCAAAATCACTATTCCGAGGTTTGCCATCTTGTTCATCGTAGTTGCCATCTTTAACTCGTTCCACCTGTTACCGCagag                                                                                     >  2:325495/1‑148 (MQ=255)
                                                           ggCTCCGTTCCTCATCCTGCTGGCGGCGCGTGTTAAACAGCTGTCTGGGGCGAACAGCGGCGAGAAAAGCAAAATCACTATTCCGAGGTTTGCCATCTTGTTCATCGTAGTTGCCATCTTTAACTCGTTCCACCTGTTACCGCAGAGc                                                                                    <  2:2574324/148‑1 (MQ=255)
                                                            gCTCCGTTCCTCATCCTGCTGGCGGCGCGTGTTAAACAGCTGTCTGGGGCGAACAGCGGCGAGAAAAGCAAAATCACTATTCCGAGGTTTGCCATCTTGTTCATCGTAGTTGCCATCTTTAACTCGTTCCACCTGTTACCGCAGAGCg                                                                                   >  1:1894989/1‑148 (MQ=255)
                                                             cTCCGTTCCTCATCCTGCTGGCGGCGCGTGTTAAACAGCTGTCTGGGGCGAACAGCGGCGAGAAAAGCAAAATCACTATTCCGAGGTTTGCCATCTTGTTCATCGTAGTTGCCATCTTTAACTCGTTCCACCTGTTACCGCAGAGCgt                                                                                  <  1:606832/148‑1 (MQ=255)
                                                                   tCCTCATCCTGCTGGCGGCGCGTGTTAAACAGCTGTCTGGGGCGAACAGCGGCGAGAAAAGCAAAATCACTATTCCGAGGTTTGCCATCTTGTTCATCGTAGTTGCCATCTTTAACTCGTTCCACCTGTTACCGCAGAGCGTGGTGaa                                                                            >  1:501881/1‑148 (MQ=255)
                                                                    ccTCATCCTGCTGGCGGCGCGTGTTAAACAGCTGTCTGGGGCGAACAGCGGCGAGAAAAGCAAAATCACTATTCCGAGGTTTGCCATCTTGTTCATCGTAGTTGCCATCTTTAACTCGTTCCACCTGTTACCGCAGAGCGTGGTGAAc                                                                           <  2:1614004/148‑1 (MQ=255)
                                                                    ccTCATCCTGCTGGCGGCGCGTGTTAAACAGCTGTCTGGGGCGAACAGCGGCGAGAAAAGCAAAATCACTATTCCGAGGTTTGCCATCTTGTTCATCGTAGTTGCCATCTTTAACTCGTTCCACCTGTTACCGCAGAGCGTGGTGAAc                                                                           <  2:1481735/148‑1 (MQ=255)
                                                                        aTCCTGCTGGCGGCGCGTGTTAAACAGCTGTCTGGGGCGAACAGCGGCGAGAAAAGCAAAATCACTATTCCGAGGTTTGCCATCTTGTTCATCGTAGTTGCCATCTTTAACTCGTTCCACCTGTTACCGCAGAGCGTGGTGAACATGc                                                                       <  2:2420700/148‑1 (MQ=255)
                                                                         tCCTGCTGGCGGCGCGTGTTAAACAGCTGTCTGGGGCGAACAGCGGCGAGAAAAGCAAAATCACTATTCCGAGGTTTGCCATCTTGTTCATCGTAGTTGCCATCTTTAACTCGTTCCACCTGTTACCGCAGAGCGTGGTGAACATGCt                                                                      >  1:1633968/1‑148 (MQ=255)
                                                                             gctgGCGGCGCGTGTTAAACAGCTGTCTGGGGCGAACAGCGGCGAGAAAAGCAAAATCACTATTCCGAGGTTTGCCATCTTGTTCATCGTAGTTGCCATCTTTAACTCGTTCCACCTGTTACCGCAGAGCGTGGTGAACATGCTGGTa                                                                  <  2:1585025/148‑1 (MQ=255)
                                                                                    gcgcGTGTTAAACAGCTGTCTGGGGCGAACAGCGGCGAGAAAAGCAAAATCACTATTCCGAGGTTTGCCATCTTGTTCATCGTAGTTGCCATCTTTAACTCGTTCCACCTGTTACCGCAGAGCGTGGTGAACATGCTGGTAACGCTgg                                                           >  2:2043390/1‑148 (MQ=255)
                                                                                        gtgtTAAACAGCTGTCTGGGGCGAACAGCGGCGAGAAAAGCAAAATCACTATTCCGAGGTTTGCCATCTTGTTCATCGTAGTTGCCATCTTTAACTCGTTCCACCTGTTACCGCAGAGCGTGGTGAACATGCTGGTAACGCTGGATAc                                                       <  1:1318444/148‑1 (MQ=255)
                                                                                         tgtTAAACAGCTGTCTGGGGCGAACAGCGGCGAGAAAAGCAAAATCACTATTCCGAGGTTTGCCATCTTGTTCATCGTAGTTGCCATCTTTAACTCGTTCCACCTGTTACCGCAGAGCGTGGTGAACATGCTGGTAACGCTGGATAcc                                                      <  1:1650218/148‑1 (MQ=255)
                                                                                          gtTAAACAGCTGTCTGGGGCGAACAGCGGCGAGAAAAGCAAAATCACTATTCCGAGGTTTGCCATCTTGTTCATCGTAGTTGCCATCTTTAACTCGTTCCACCTGTTACCGCAGAGCGTGGTGAACATGCTGGTAACGCTGGATACCt                                                     <  1:57297/148‑1 (MQ=255)
                                                                                           ttAAACAGCTGTCTGGGGCGAACAGCGGCGAGAAAAGCAAAATCACTATTCCGAGGTTTGCCATCTTGTTCATCGTAGTTGCCATCTTTAACTCGTTCCACCTGTTACCGCAGAGCGTGGTGAACATGCTGGTAACGCTGGATACCtt                                                    >  2:66835/1‑148 (MQ=255)
                                                                                           ttAAACAGCTGTCTGGGGCGAACAGCGGCGAGAAAAGCAAAATCACTATTCCGAGGTTTGCCATCTTGTTCATCGTAGTTGCCATCTTTAACTCGTTCCACCTGTTACCGCAGAGCGTGGTGAACATGCTGGTAACGCTGGATACCtt                                                    >  2:1844934/1‑148 (MQ=255)
                                                                                            tAAACAGCTGTCTGGGGCGAACAGCGGCGAGAAAAGCAAAATCACTATTCCGAGGTTTGCCATCTTGTTCATCGTAGTTGCCATCTTTAACTCGTTCCACCTGTTACCGCAGAGCGTGGTGAACATGCTGGTAACGCTGGATACCTTc                                                   <  2:676805/148‑1 (MQ=255)
                                                                                            tAAACAGCTGTCTGGGGCGAACAGCGGCGAGAAAAGCAAAATCACTATTCCGAGGTTTGCCATCTTGTTCATCGTAGTTGCCATCTTTAACTCGTTCCACCTGTTACCGCAGAGCGTGGTGAACATGCTGGTAACGCTGGATACCTTc                                                   >  2:3072842/1‑148 (MQ=255)
                                                                                               aCAGCTGTCTGGGGCGAACAGCGGCGAGAAAAGCAAAATCACTATTCCGAGGTTTGCCATCTTGTTCATCGTAGTTGCCATCTTTAACTCGTTCCACCTGTTACCGCAGAGCGTGGTGAACATGCTGGTAACGCTGGATACCTTCctg                                                <  1:717932/148‑1 (MQ=255)
                                                                                               aCAGCTGTCTGGGGCGAACAGCGGCGAGAAAAGCAAAATCACTATTCCGAGGTTTGCCATCTTGTTCATCGTAGTTGCCATCTTTAACTCGTTCCACCTGTTACCGCAGAGCGTGGTGAACATGCTGGTAACGCTGGATACCTTCctg                                                >  1:2208087/1‑148 (MQ=255)
                                                                                               aCAGCTGTCTGGGGCGAACAGCGGCGAGAAAAGCAAAATCACTATTCCGAGGTTTGCCATCTTGTTCATCGTAGTTGCCATCTTTAACTCGTTCCACCTGTTACCGCAGAGCGTGGTGAACATGCTGGTAACGCTGGATACCTTCctg                                                <  1:3318625/148‑1 (MQ=255)
                                                                                                 aGCTGTCTGGGGCGAACAGCGGCGAGAAAAGCAAAATCACTATTCCGAGGTTTGCCATCTTGTTCATCGTAGTTGCCATCTTTAACTCGTTCCACCTGTTACCGCAGAGCGTGGTGAACATGCTGGTAACGCTGGATACCTTCctgct                                              >  1:2919726/1‑148 (MQ=255)
                                                                                                 aGCTGTCTGGGGCGAACAGCGGCGAGAAAAGCAAAATCACTATTCCGAGGTTTGCCATCTTGTTCATCGTAGTTGCCATCTTTAACTCGTTCCACCTGTTACCGCAGAGCGTGGTGAACATGCTGGTAACGCTGGATACCTTCctgct                                              <  2:513682/148‑1 (MQ=255)
                                                                                                      tCTGGGGCGAACAGCGGCGAGAAAAGCAAAATCACTATTCCGAGGTTTGCCATCTTGTTCATCGTAGTTGCCATCTTTAACTCGTTCCACCTGTTACCGCAGAGCGTGGTGAACATGCTGGTAACGCTGGATACCTTCCTGCTGGCaa                                         <  1:1424907/148‑1 (MQ=255)
                                                                                                      tCTGGGGCGAACAGCGGCGAGAAAAGCAAAATCACTATTCCGAGGTTTGCCATCTTGTTCATCGTAGTTGCCATCTTTAACTCGTTCCACCTGTTACCGCAGAGCGTGGTGAACATGCTGGTAACGCTGGATACCTTCCTGCTGGCaa                                         <  2:3379279/148‑1 (MQ=255)
                                                                                                      tCTGGGGCGAACAGCGGCGAGAAAAGCAAAATCACTATTCCGAGGTTTGCCATCTTGTTCATCGTAGTTGCCATCTTTAACTCGTTCCACCTGTTACCGCAGAGCGTGGTGAACATGCTGGTAACGCTGGATACCTTCCTGCTGGCaa                                         <  1:863598/148‑1 (MQ=255)
                                                                                                      tCTGGGGCGAACAGCGGCGAGAAAAGCAAAATCACTATTCCGAGGTTTGCCATCTTGTTCATCGTAGTTGCCATCTTTAACTCGTTCCACCTGTTACCGCAGAGCGTGGTGAACATGCTGGTAACGCTGGATACCTTCCTGCTGGCaa                                         <  1:2867894/148‑1 (MQ=255)
                                                                                                         ggggCGAACAGCGGCGAGAAAAGCAAAATCACTATTCCGAGGTTTGCCATCTTGTTCATCGTAGTTGCCATCTTTAACTCGTTCCACCTGTTACCGCAGAGCGTGGTGAACATGCTGGTAACGCTGGATACCTTCCTGCTGGCAatgg                                      <  2:56890/148‑1 (MQ=255)
                                                                                                            gCGAACAGCGGCGAGAAAAGCAAAATCACTATTCCGAGGTTTGCCATCTTGTTCATCGTAGTTGCCATCTTTAACTCGTTCCACCTGTTACCGCAGAGCGTGGTGAACATGCTGGTAACGCTGGATACCTTCCTGCTGGCAatggcga                                   <  1:1926241/148‑1 (MQ=255)
                                                                                                               aaCATCGGCGAGAAAAGCAAAATCACTATTCAGAGGTTTGCCATCTTGTTCATCGTAGTTGCCATCTTTAACTCGTTCCACCTGTTACCGCAGAGCGTGGTGAACATTCTGGTAACGCTGGATACCTTCCTGCTGGCAatggcgatgg                                <  1:1374330/148‑1 (MQ=255)
                                                                                                               aaCAGCGGCGAGAAAAGCAAAATCACTATTCCGAGGTTTGCCATCTTGTTCCTCGTAGTTGCCATCTTTAACTCGTTCCACCTGTTACCGCAGAGCGTGGTGAACATGCTGGTAACGCTGGATACCTTCCTGCTGGCAatggcgatgg                                <  2:2168374/148‑1 (MQ=255)
                                                                                                               aaCAGCGGCGAGAAAAGCAAAATCACTATTCCGAGGTTTGCCATCTTGTTCATCGTAGTTGCCATCTTTAACTCGTTCCACCTGTTACCGCAGAGCGTGGTGAACATGCTGGTAACGCTGGATACCTTCCTGCTGGCAatggcgatgg                                >  2:230987/1‑148 (MQ=255)
                                                                                                               aaCAGCGGCGAGAAAAGCAAAATCACTATTCCGAGGTTTGCCATCTTGTTCATCGTAGTTGCCATCTTTAACTCGTTCCACCTGTTACCGCAGAGCGTGGTGAACATGCTGGTAACGCTGGATACCTTCCTGCTGGCAatggcgatgg                                <  2:2167834/148‑1 (MQ=255)
                                                                                                                 cAGCGGCGAGAAAAGCAAAATCACTATTCCGAGGTTTGCCATCTTGTTCATCGTAGTTGCCATCTTTAACTCGTTCCACCTGTTACCGCAGAGCGTGGTGAACATGCTGGTAACGCTGGATACCTTCCTGCTGGCAatggcgatggcg                              <  1:3506540/148‑1 (MQ=255)
                                                                                                                      gcgAGAAAAGCAAAATCACTATTCCGAGGTTTGCCATCTTGTTCATCGTAGTTGCCATCTTTAACTCGTTCCACCTGTTACCGCAGAGCGTGGTGAACATGCTGGTAACGCTGGATACCTTCCTGCTGGCAATGGCGATGGCGGCGCt                         >  1:2330067/1‑148 (MQ=255)
                                                                                                                       cgAGAAAAGCAAAATCACTATTCCGAGGTTTGCCATCTTGTTCATCGTAGTTGCCATCTTTAACTCGTTCCACCTGTTACCGCAGAGCGTGGTGAACATGCTGGTAACGCTGGATACCTTCCTGCTGGCAATGGCGATGGCGGCGCTg                        >  1:252163/1‑148 (MQ=255)
                                                                                                                          gaAAAGCAAAATCACTATTCCGAGGTTTGCCATCTTGTTCATCGTAGTTGCCATCTTTAACTCGTTCCACCTGTTACCGCAGAGCGTGGTGAACATGCTGGTAACGCTGGATACCTTCCTGCTGGCAATGGCGATGGCGGCGCTGGGt                     <  1:1067798/148‑1 (MQ=255)
                                                                                                                           aaaaGCAAAATCACTATTCCGAGGTTTGCCATCTTGTTCATCGTAGTTGCCATCTTTAACTCGTTCCACCTGTTACCGCAGAGCGTGGTGAACATGCTGGTAACGCTGGATACCTTCCTGCTGGCAATGGCGATGGCGGCGCTGGGTc                    >  1:933254/1‑148 (MQ=255)
                                                                                                                           aaaaGCAAAATCACTATTCCGAGGTTTGCCATCTTGTTCATCGTAGTTGCCATCTTTAACTCGTTCCACCTGTTACCGCAGAGCGTGGTGAACATGCTGGTAACGCTGGATACCTTCCTGCTGGCAATGGCGATGGCGGCGCTGGGTc                    >  2:481730/1‑148 (MQ=255)
                                                                                                                           aaaaGCAAAATCACTATTCCGAGGTTTGCCATCTTGTTCATCGTAGTTGCCATCTTTAACTCGTTCCACCTGTTACCGCAGAGCGTGGTGAACATGCTGGTAACGCTGGATACCTTCCTGCTGGCAATGGCGATGGCGGCGCTGGGTc                    >  2:1401109/1‑148 (MQ=255)
                                                                                                                             aaGCAAAATCACTATTCCGAGGTTTGCCATCTTGTTCATCGTAGTTGCCATCTTTAACTCGTTCCACCTGTTACCGCAGAGCGTGGTGAACATGCTGGTAACGCTGGATACCTTCCTGCTGGCAATGGCGATGGCGGCGCTGGGTCTg                  >  2:3121755/1‑148 (MQ=255)
                                                                                                                              aGCAAAATCACTATTCCGAGGTTTGCCATCTTGTTCATCGTAGTTGCCATCTTTAACTCGTTCCACCTGTTACCGCAGAGCGTGGTGAACATGCTGGTAACGCTGGATACCTTCCTGCTGGCAATGGCGATGGCGGCGCTGGGTCTGa                 >  1:459243/1‑148 (MQ=255)
                                                                                                                                   aaTCACTATTCCGAGGTTTGCCATCTTGTTCATCGTAGTTGCCATCTTTAACTCGTTCCACCTGTTACCGCAGAGCGTGGTGAACATGCTGGTAACGCTGGATACCTTCCTGCTGGCAATGGCGATGGCGGCGCTGGGTCTGACCACt            >  1:1492973/1‑148 (MQ=255)
                                                                                                                                   aaTCACTATTCCGAGGTTTGCCATCTTGTTCATCGTAGTTGCCATCTTTAACTCGTTCCACCTGTTACCGCAGAGCGTGGTGAACATGCTGGTAACGCTGGATACCTTCCTGCTGGCAATGGCGATGGCGGCGCTGGGTCTGACCACt            >  1:2534881/1‑148 (MQ=255)
                                                                                                                                     tCACTATTCCGAGGTTTGCCATCTTGTTCATCGTAGTTGCCATCTTTAACTCGTTCCACCTGTTACCGCAGAGCGTGGTGAACATGCTGGTAACGCTGGATACCTTCCTGCTGGCAATGGCGATGGCGGCGCTGGGTCTGACCACTCa          <  1:3197738/148‑1 (MQ=255)
                                                                                                                                     tCACTATTCCGAGGTTTGCCATCTTGTTCATCGTAGTTGCCATCTTTAACTCGTTCCACCTGTTACCGCAGAGCGTGGTGAACATGCTGGTAACGCTGGATACCTTCCTGCTGGCAATGGCGATGGCGGCGCTGGGTCTGACCACTCa          >  2:3322185/1‑148 (MQ=255)
                                                                                                                                      cACTATTCCGAGGTTTGCCATCTTGTTCATCGTAGTTGCCATCTTTAACTCGTTCCACCTGTTACCGCAGAGCGTGGTGAACATGCTGGTAACGCTGGATACCTTCCTGCTGGCAATGGCGATGGCGGCGCTGGGTCTGACCACTCAc         >  2:2870318/1‑148 (MQ=255)
                                                                                                                                       aCTATTACGAGGTTTGCCATCTTGTTCATCGTAGTTGCCATCTTAAACTAGTTCCACCTGTTACCGCAGAGCGTGGTGAACATGCTGGTAACGCTGGATACCTTCCTGCTGGCAATGGCGATGGCGGCGCTGGGTCTGACCACTCACg        >  1:2666580/1‑148 (MQ=255)
                                                                                                                                          aTTCCGAGGTTTGCCATCTTGTTCATCGTAGTTGCCATCTTTAACTCGTTCCACCTGTTACCGCAGAGCGTGGTGAACATGCTGGTAACGCTGGATACCTTCCTGCTGGCAATGGCGATGGCGGCGCTGGGTCTGACCACTCACGTCa     >  1:1173573/1‑148 (MQ=255)
                                                                                                                                          aTTCCGAGGTTTGCCATCTTGTTCATCGTAGTTGCCATCTTTAACTCGTTCCACCTGTTACCGCAGAGCGTGGTGAACATGCTGGTAACGCTGGATACCTTCCTGCTGGCAATGGCGATGGCGGCGCTGGGTCTGACCACTCACGTCa     <  1:1057540/148‑1 (MQ=255)
                                                                                                                                          aTTCCGAGGTTTGCCATCTTGTTCATCGTAGTTGCCATCTTTAACTCGTTCCACCTGTTACCGCAGAGCGTGGTGAACATGCTGGTAACGCTGGATACCTTCCTGCTGGCAATGGCGATGGCGGCGCTGGGTCTGACCACTCACGTCa     >  1:681125/1‑148 (MQ=255)
                                                                                                                                           ttCCGAGGTTTGCCATCTTGTTCATCGTAGTTGCCATCTTTAACTCGTTCCACCTGTTACCGCAGAGCGTGGTGAACATGCTGGTAACGCTGGATACCTTCCTGCTGGCAATGGCGATGGCGGCGCTGGGTCTGACCACTCACGTCAg    >  2:2554299/1‑148 (MQ=255)
                                                                                                                                           ttCCGAGGTTTGCCATCTTGTTCATCGTAGTTGCCATCTTTAACTCGTTCCACCTGTTACCGCAGAGCGTGGTGAACATGCTGGTAACGCTGGATACCTTCCTGCTGGCAATGGCGATGGCGGCGCTGGGTCTGACCACTCACGTCAg    >  2:2554475/1‑148 (MQ=255)
                                                                                                                                            tCCGAGGTTTGCCATCTTGTTCATCGTAGTTGCCATCTTTAACTCGTTCCACCTGTTACCGCAGAGCGTGGTGAACATGCTGGTAACGCTGGATACCTTCCTGCTGGCAATGGCGATGGCGGCGCTGGGTCTGACCACTCACGTCAgc   >  2:3048979/1‑148 (MQ=255)
                                                                                                                                            tCCGAGGTTTGCCATCTTGTTCATCGTAGTTGCCATCTTTAACTCGTTCCACCTGTTACCGCAGAGCGTGGTGAACATGCTGGTAACGCTGGATACCTTCCTGCTGGCAATGGCGATGGCGGCGCTGGGTCTGACCACTCACGTCAgc   >  2:2587071/1‑148 (MQ=255)
                                                                                                                                             ccGAGGTTTGCCATCTTGTTCATCGTAGTTGCCATCTTTAACTCGTTCCACCTGTTACCGCAGAGCGTGGTGAACATGCTGGTAACGCTGGATACCTTCCTGCTGGCAATGGCGATGGCGGCGCTGGGTCTGACCACTCACGTCAgcg  <  2:191174/148‑1 (MQ=255)
                                                                                                                                             ccGAGGTTTGCCATCTTGTTCATCGTAGTTGCCATCTTTAACTCGTTCCACCTGTTACCGCAGAGCGTGGTGAACATGCTGGTAACGCTGGATACCTTCCTGCTGGCAATGGCGATGGCGGCGCTGGGTCTGACCACTCACGTCAgcg  >  2:2230444/1‑148 (MQ=255)
                                                                                                                                             ccGAGGTTTGCCATCTTGTTCATCGTAGTTGCCATCTTTAACTCGTTCCACCTGTTACCGCAGAGCGTGGTGAACATGCTGGTAACGCTGGATACCTTCCTGCTGGCAATGGCGATGGCGGCGCTGGGTCTGACCACTCACGTCAgcg  >  2:2615846/1‑148 (MQ=255)
                                                                                                                                                |                                                                                                                                                
ATCAGCCCGGATGCGGAAAACGCAGCAGTTATTTCCAAAATGCTGCGCGTGATGATGCTGGCTCCGTTCCTCATCCTGCTGGCGGCGCGTGTTAAACAGCTGTCTGGGGCGAACAGCGGCGAGAAAAGCAAAATCACTATTCCGTGGTTTGCCATCTTGTTCATCGTAGTTGCCATCTTTAACTCGTTCCACCTGTTACCGCAGAGCGTGGTGAACATGCTGGTAACGCTGGATACCTTCCTGCTGGCAATGGCGATGGCGGCGCTGGGTCTGACCACTCACGTCAGCG  >  CP009273/2243847‑2244135

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TGCCATCAGCCCGGATGCGGAAAACGCAGCAGTTATTTCCAAAATGCTGCGCGTGATGATGCTGGCTCCGTTCCTCATCCTGCTGGCGGCGCGTGTTAAACAGCTGTCTGGGGCGAACAGCGGCGAGAAAAGCAAAATCACTATTCCGTGGTTTGCCATCTTGTTCATCGTAGTTGCCATCTTTAACTCGTTCCACCTGTTACCGCAGAGCGTGGTGAACATGCTGGTAACGCTGGATACCTTCCTGCTGGCAATGGCGATGGCGGCGCTGGGTCTGACCACTCACGTCAGC  >  CP009273/2243843‑2244134
                                                                                                                                                    |                                                                                                                                               
TGCCATCAGCCCGGATGCGGAAAACGCAGCAGTTATTTCCAAAATGCTGCGCGTGATGATGCTGGCTCCGTTCCTCATCCTGCTGGCGGCGCGTGTTAAACAGCTGTCTGGGGCGAACAGCGGCGAGAAAAGCAAAATCACTATTCCGAG                                                                                                                                                <  SRR19973065.1390468/150‑1 (MQ=60)
 GCCATCAGCCCGGATGCGGAAAACGCAGCAGTTATTTCCAAAATGCTGCGCGTGATGATGCTGGCTCCGTTCCTCATCCTGCTGGCGGCGCGTGTTAAACAGCTGTCTGGGGCGAACAGCGGCGAGAAAAGCAAAATCACTATTCCGAGG                                                                                                                                               <  SRR19973065.1078412/150‑1 (MQ=60)
 GCCATCAGCCCGGATGCGGAAAACGCAGCAGTTATTTCCAAAATGCTGCGCGTGATGATGCTGGCTCCGTTCCTCATCCTGCTGGCGGCGCGTGTTAAACAGCTGTCTGGGGCGAACAGCGGCGAGAAAAGCAAAATCACTATTCCGAGG                                                                                                                                               <  SRR19973065.817804/150‑1 (MQ=60)
  CCATCAGCCCGGATGCGGAAAACGCAGCAGTTATTTCCAAAATGCTGCGCGTGATGATGCTGGCTCCGTTCCTCATCCTGCTGGCGGCGCGTGTTAAACAGCTGTCTGGGGCGAACAGCGGCGAGAAAAGCAAAATCACTATTCCGAGGT                                                                                                                                              >  SRR19973065.2955706/1‑150 (MQ=60)
  CCATCAGCCCGGATGCGGAAAACGCAGCAGTTATTTCCAAAATGCTGCGCGTGATGATGCTGGCTCCGTTCCTCATCCTGCTGGCGGCGCGTGTTAAACAGCTGTCTGGGGCGAACAGCGGCGAGAAAAGCAAAATCACTATTCCGAGGT                                                                                                                                              >  SRR19973065.679671/1‑150 (MQ=60)
    ATCAGCCCGGATGCGGAAAACGCAGCAGTTATTTCCAAAATGCTGCGCGTGATGATGCTGGCTCCGTTCCTCATCCTGCTGGCGGCGCGTGTTAAACAGCTGTCTGGGGCGAACAGCGGCGAGAAAAGCAAAATCACTATTCCGAGGTTT                                                                                                                                            >  SRR19973065.2775928/1‑150 (MQ=60)
     TCAGCCCGGATGCGGAAAACGCAGCAGTTATTTCCAAAATGCTGCGCGTGATGATGCTGGCTCCGTTCCTCATCCTGCTGGCGGCGCGTGTTAAACAGCTGTCTGGGGCGAACAGCGGCGAGAAAAGCAAAATCACTATTCCGAGGTTTG                                                                                                                                           >  SRR19973065.3330053/1‑150 (MQ=60)
      CAGCCCGGATGCGGAAAACGCAGCAGTTATTTCCAAAATGCTGCGCGTGATGATGCTGGCTCCGTTCCTCATCCTGCTGGCGGCGCGTGTTAAACAGCTGTCTGGGGCGAACAGCGGCGAGAAAAGCAAAATCACTATTCCGAGGTTTGC                                                                                                                                          <  SRR19973065.2659815/150‑1 (MQ=60)
       AGCCCGGATGCGGAAAACGCAGCAGTTATTTCCAAAATGCTGCGCGTGATGATGCTGGCTCCGTTCCTCATCCTGCTGGCGGCGCGTGTTAAACAGCTGTCTGGGGCGAACAGCGGCGAGAAAAGCAAAATCACTATTCCGAGGTTTGCC                                                                                                                                         <  SRR19973065.2509362/150‑1 (MQ=60)
       AGCCCGGATGCGGAAAACGCAGCAGTTAGTTCCAAAATGCTGCGCGTGATGATGCTGGCTCCGGTCCTCATCCTGCTGGCGGCGCGTGTTAAACAGCTGTCTGGGGCGAACAGCGGCGAGAAAAGCAAAATCACTATTCCGAGGTTTGCC                                                                                                                                         <  SRR19973065.2155273/150‑1 (MQ=60)
         CCCGGATGCGGAAAACGCAGCAGTTATTTCCAAAATGCTGCGCGTGATGATGCTGGCTCCGTTCCTCATCCTGCTGGCGGCGCGTGTTAAACAGCTGTCTGGGGCGAACAGCGGCGAGAAAAGCAAAATCACTATTCCGAGGTTTGCCAT                                                                                                                                       >  SRR19973065.3321814/1‑150 (MQ=60)
         CCCGGATGCGGAAAACGCAGCAGTTATTTCCAAAATGCTGCGCGTGATGATGCTGGCTCCGTTCCTCATCCTGCTGGCGGCGCGTGTTAAACAGCTGTCTGGGGCGAACAGCGGCGAGAAAAGCAAAATCACTATTCCGAGGTTTGCCAT                                                                                                                                       >  SRR19973065.246257/1‑150 (MQ=60)
         CCCGGATGCGGAAAACGCAGCAGTTATTTCCAAAATGCTGCGCGTGATGATGCTGGCTCCGTTCCTCATCCTGCTGGCGGCGCGTGTTAAACAGCTGTCTGGGGCGAACAGCGGCGAGAAAAGCAAAATCACTATTCCGAGGTTTGCCAT                                                                                                                                       >  SRR19973065.2030841/1‑150 (MQ=60)
            GGATGCGGAAAACGCAGCAGTTATTTCCAAAATGCTGCGCGTGATGATGCTGGCTCCGTTCCTCATCCTGCTGGCGGCGCGTGTTAAACAGCTGTCTGGGGCGAACAGCGGCGAGAAAAGCAAAATCACTATTCCGAGGTTTGCCATCTT                                                                                                                                    <  SRR19973065.955774/150‑1 (MQ=60)
            GGATGCGGAAAACGCAGCAGTTATTTCCAAAATGCTGCGCGTGATGATGCTGGCTCCGTTCCTCATCCTGCTGGCGGCGCGTGTTAAACAGCTGTCTGGGGCGAACAGCGGCGAGAAAAGCAAAATCACTATTCCGAGGTTTGCCATCTT                                                                                                                                    >  SRR19973065.1145732/1‑150 (MQ=60)
             GATGCGGAAAACGCAGCAGTTATTTCCAAAATGCTGCGCGTGATGATGCTGGCTCCGTTCCTCATCCTGCTGGCGGCGCGTGTTAAACAGCTGTCTGGGGCGAACAGCGGCGAGAAAAGCAAAATCACTATTCCGAGGTTTGCCATCTTG                                                                                                                                   >  SRR19973065.2130823/1‑150 (MQ=60)
                  GGAAAACGCAGCAGTTATTTCCAAAATGCTGCGCGTGATGATGCTGGCTCCGTTCCTCATCCTGCTGGCGGCGCGTGTTAAACAGCTGTCTGGGGCGAACAGCGGCGAGAAAAGCAAAATCACTATTCCGAGGTTTGCCATCTTGTTCAT                                                                                                                              >  SRR19973065.2857864/1‑150 (MQ=60)
                   GAAAACGCAGCAGTTATTTCCAAAATGCTGCGCGTGATGATGCTGGCTCCGTTCCTCATCCTGCTGGCGGCGCGTGTTAAACAGCTGTCTGGGGCGAACAGCGGCGAGAAAAGCAAAATCACTATTCCGTGGTTTGCCATCTTGTTCATC                                                                                                                             <  SRR19973065.1825998/150‑1 (MQ=60)
                   GAAAACGCAGCAGTTATTTCCAAAATGCTGCGCGTGATGATGCTGGCTCCGTTCCTCATCCTGCTGGCGGCGCGTGTTAAACAGCTGTCTGGGGCGAACAGCGGCGAGAAAAGCAAAATCACTATTCCGAGGTTTGCCATCTTGTTCATC                                                                                                                             <  SRR19973065.2998271/150‑1 (MQ=60)
                    AAAACGCAGCAGTTATTTCCAAAATGCTGCGCGTGATGATGCTGGCTCCGTTCCTCATCCTGCTGGCGGCGCGTGTTAAACAGCTGTCTGGGGCGAACAGCGGCGAGAAAAGCAAAATCACTATTCCGTGGTTTGCCATCTTGTTCATCG                                                                                                                            >  SRR19973065.24811/1‑150 (MQ=60)
                      AACGCAGCAGTTATTTCCAAAATGCTGCGCGTGATGATGCTGGCTCCGTTCCTCATCCTGCTGGCGGCGCGTGTTAAACAGCTGTCTGGGGCGAACAGCGGCGAGAAAAGCAAAATCACTATTCCGAGGTTTGCCATCTTGTTCATCGTA                                                                                                                          >  SRR19973065.192670/1‑150 (MQ=60)
                         GCAGCAGTTATTTCCAAAATGCTGCGCGTGATGATGCTGGCTCCGTTCCTCATCCTGCTGGCGGCGCGTGTTAAACAGCTGTCTGGGGCGAACAGCGGCGAGAAAAGCAAAATCACTATTCCGAGGTTTGCCATCTTGTTCATCGTAGTT                                                                                                                       <  SRR19973065.1960260/150‑1 (MQ=60)
                         GCAGCAGTTATTTCCAAAATGCTGCGCGTGATGATGCTGGCTCCGTTCCTCATCCTGCTGGCGGCGCGTGTTAAACAGCTGTCTGGGGCGAACAGCGGCGAGAAAAGCAAAATCACTATTCCGAGGTTTGCCATCTTGTTCATCGTAGTT                                                                                                                       <  SRR19973065.1703642/150‑1 (MQ=60)
                          CAGCAGTTATTTCCAAAATGCTGCGCGTGATGATGCTGGCTCCGTTCCTCATCCTGCTGGCGGCGCGTGTTAAACAGCTGTCTGGGGCGAACAGCGGCGAGAAAAGCAAAATCACTATTCCGAGGTTTGCCATCTTGTTCATCGTAGTTG                                                                                                                      <  SRR19973065.2948582/150‑1 (MQ=60)
                          CAGCAGTTATTTCCAAAATGCTGCGCGTGATGATGCTGGCTCCGTTCCTCATCCTGCTGGCGGCGCGTGTTAAACAGCTGTCTGGGGCGAACAGCGGCGAGAAAAGCAAAATCACTATTCCGAGGTTTGCCATCTTGTTCATCGTAGTTG                                                                                                                      <  SRR19973065.3448771/150‑1 (MQ=60)
                          CAGCAGTTATTTCCAAAATGCTGCGCGTGATGATGCTGGCTCCGTTCCTCATCCTGCTGGCGGCGCGTGTTAAACAGCTGTCTGGGGCGAACAGCGGCGAGAAAAGCAAAATCACTATTCCGAGGTTTGCCATCTTGTTCATCGTAGTTG                                                                                                                      <  SRR19973065.2712108/150‑1 (MQ=60)
                          CAGCAGTTATTTCCAAAATGCTGCGCGTGATGATGCTGGCTCCGTTCCTCATCCTGCTGGCGGCGCGTGTTAAACAGCTGTCTGGGGCGAACAGCGGCGAGAAAAGCAAAATCACTATTCCGAGGTTTGCCATCTTGTTCATCGTAGTTG                                                                                                                      <  SRR19973065.3448852/150‑1 (MQ=60)
                                TTATTTCCAATATGCTGCGCGTGATGATTCTGGCTCCTTTCCTCATCCTGGTGGCGGCGCGTGTTAATAATCTGTCTGGGGCGAACAGCGGCGATAATAGCAAAATCACTATTCCGAGGTTTGCCATCTTGTTCATCGTATTTGCCATCT                                                                                                                <  SRR19973065.697379/150‑1 (MQ=60)
                                TTATTTCCAAAATGCTGCGCGTGATGATGCTGGCTCCGTTCCTCATCCTGCTGGCGGCGCGTGTTAAACAGCTGTCTGGGGCGAACAGCGGCGAGAAAAGCAAAATCACTATTCCGAGGTTTGCCATCTTGTTCATCGTAGTTGCCATCT                                                                                                                <  SRR19973065.2391444/150‑1 (MQ=60)
                                 TATTTCCAAAATGCTGCGCGTGATGATGCTGGCTCCGTTCCTCATCCTGCTGGCGGCGCGTGTTAAACAGCTGTCTGGGGCGAACAGCGGCGAGAAAAGCAAAATCACTATTCCGAGGTTTGCCATCTTGTTCATCGTAGTTGCCATCTT                                                                                                               <  SRR19973065.1708711/150‑1 (MQ=60)
                                 TATTTCCAAAATGCTGCGCGTGATGATGCTGGCTCCGTTCCTCATCCTGCTGGCGGCGCGTGTTAAACAGCTGTCTGGGGCGAACAGCGGCGAGAAAAGCAAAATCACTATTCCGAGGTTTGCCATCTTGTTCATCGTAGTTGCCATCTT                                                                                                               <  SRR19973065.1708371/150‑1 (MQ=60)
                                   TTTCCAAAATGCTGCGCGTGATGATGCTGGCTCCGTTCCTCATCCTGCTGGCGGCGCGTGTTAAACAGCTGTCTGGGGCGAACAGCGGCGAGAAAAGCAAAATCACTATTCCGAGGTTTGCCATCTTGTTCATCGTAGTTGCCATCTTTA                                                                                                             <  SRR19973065.2754685/150‑1 (MQ=60)
                                       CAAAATGCTGCGCGTGATGATGCTGGCTCCGTTCCTCATCCTGCTGGCGGCGCGTGTTAAACAGCTGTCTGGGGCGAACAGCGGCGAGAAAAGCAAAATCACTATTCCGAGGTTTGCCATCTTGTTCATCGTAGTTGCCATCTTTAACTC                                                                                                         >  SRR19973065.2786734/1‑150 (MQ=60)
                                       CAAAATGCTGCGCGTGATGATGCTGGCTCCGTTCCTCATCCTGCTGGCGGCGCGTGTTAAACAGCTGTCTGGGGCGAACAGCGGCGAGAAAAGCAAAATCACTATTCCGAGGTTTGCCATCTTGTTCATCGTAGTTGCCATCTTTAACTC                                                                                                         >  SRR19973065.2786786/1‑150 (MQ=60)
                                          AATGCTGCGCGTGATGATGCTGGCTCCGTTCCTCATCCTGCTGGCGGCGCGTGTTAAACAGCTGTCTGGGGCGAACAGCGGCGAGAAAAGCAAAATCACTATTCCGAGGTTTGCCATCTTGTTCATCGTAGTTGCCATCTTTAACTCGTT                                                                                                      >  SRR19973065.2661068/1‑150 (MQ=60)
                                          AATGCTGCGCGTGATGATGCTGGCTCCGTTCCTCATCCTGCTGGCGGCGCGTGTTAAACAGCTGTCTGGGGCGAACAGCGGCGAGAAAAGCAAAATCACTATTCCGAGGTTTGCCATCTTGTTCATCGTAGTTGCCATCTTTAACTCGTT                                                                                                      >  SRR19973065.735470/1‑150 (MQ=60)
                                             GCTGCGCGTGATGATGCTGGCTCCGTTCCTCATCCTGCTGGCGGCGCGTGTTAAACAGCTGTCTGGGGCGAACAGCGGCGAGAAAAGCAAAATCACTATTCCGAGGTTTGCCATCTTGTTCATCGTAGTTGCCATCTTTAACTCGTTCCA                                                                                                   <  SRR19973065.3132619/150‑1 (MQ=60)
                                             GCTGCGCGTGATGATGCTGGCTCCGTTCCTCATCCTGCTGGCGGCGCGTGTTAAACAGCTGTCTGGGGCGAACAGCGGCGAGAAAAGCAAAATCACTATTCCGAGGTTTGCCATCTTGTTCATCGTAGTTGCCATCTTTAACTCGTTCCA                                                                                                   <  SRR19973065.297763/150‑1 (MQ=60)
                                             GCTGCGCGTGATGATGCTGGCTCCGTTCCTCATCCTGCTGGCGGCGCGTGTTAAACAGCTGTCTGGGGCGAACAGCGGCGAGAAAAGCAAAATCACTATTCCGAGGTTTGCCATCTTGTTCATCGTAGTTGCCATCTTTAACTCGTTCCA                                                                                                   <  SRR19973065.2602257/150‑1 (MQ=60)
                                               TGCGCGTGATGATGCTGGCTCCGTTCCTCATCCTGCTGGCGGCGCGTGTTAAACAGCTGTCTGGGGCGAACAGCGGCGAGAAAAGCAAAATCACTATTCCGAGGTTTGCCATCTTGTTCATCGTAGTTGCCATCTTTAACTCGTTCCACC                                                                                                 >  SRR19973065.2838355/1‑150 (MQ=60)
                                                GCGCGTGATGATGCTGGCTCCGTTCCTCATCCTGCTGGCGGCGCGTGTTAAACAGCTGTCTGGGGCGAACAGCGGCGAGAAAAGCAAAATCACTATTCCGAGGTTTGCCATCTTGTTCATCGTAGTTGCCATCTTTAACTCGTTCCACCT                                                                                                <  SRR19973065.1877331/150‑1 (MQ=60)
                                                GCGCGTGATGATGCTGGCTCCGTTCCTCATCCTGCTGGCGGCGCGTGTTAAACAGCTGTCTGGGGCGAACAGCGGCGAGAAAAGCAAAATCACTATTCCGAGGTTTGCCATCTTGTTCATCGTAGTTGCCATCTTTAACTCGTTCCACCT                                                                                                <  SRR19973065.2908284/150‑1 (MQ=60)
                                                 CGCGTGATGATGCTGGCTCCGTTCCTCATCCTGCTGGCGGCGCGTGTTAAACAGCTGTCTGGGGCGAACAGCGGCGAGAAAAGCAAAATCACTATTCCGAGGTTTGCCATCTTGTTCATCGTAGTTGCCATCTTTAACTCGTTCCACCTG                                                                                               <  SRR19973065.1750850/150‑1 (MQ=60)
                                                   CNTGATGATGCTGGCTCCGTTCCTCATCCTGCTGGCGGCGCGTGTTAAACAGCTGTCTGGGGCGAACAGCGGCGAGAAAAGCAAAATCACTATTCCGAGGTTTGCCATCTTGTTCATCGTAGTTGCCATCTTTAACTCGTTCCACCTGTT                                                                                             >  SRR19973065.3011798/1‑150 (MQ=60)
                                                   CGTGATGATGCTGTCTCCGTTCCTCATCCTGCTTTCTGCGCGTGTTAAAAAGCTGTATTGTGCGAACAGCGGCGAGAAAAGCAAAATCACTATTCCGAGGTTTGCCATCTTGTTCATAGTATTTTCCATCTTTAACTCGTTCAACCTGTT                                                                                             <  SRR19973065.1804033/150‑1 (MQ=60)
                                                   CGTGATGATGCTGGCTCCGTTCCTCATCCTGCTGGCGGCGCGTGTTAAACAGCTGTCTGGGGCGAACAGCGGCGAGAAAAGCAAAATCACTATTCCGAGGTTTGCCATCTTGTTCATCGTAGTTGCCATCTTTAACTCGTTCCACCTGTT                                                                                             >  SRR19973065.1248285/1‑150 (MQ=60)
                                                              TGGCTCCGTTCCTCATCCTGCTGGCGGCGCGTGTTAAACAGCTGTCTGGGGCGAACAGCGGCGAGAAAAGCAAAATCACTATTCCGAGGTTTGCCATCTTGTTCATCGTAGTTGCCATCTTTAACTCGTTCCACCTGTTACCGCAGAGCG                                                                                  >  SRR19973065.1907440/1‑150 (MQ=60)
                                                               GGCTCCGTTCCTCATCCTGCTGGCGGCGCGTGTTAAACAGCTGTCTGGGGCGAACAGCGGCGAGAAAAGCAAAATCACTATTCCGAGGTTTGCCATCTTGTTCATCGTAGTTGCCATCTTTAACTCGTTCCACCTGTTACCGCAGAGCGT                                                                                 >  SRR19973065.94213/1‑150 (MQ=60)
                                                                GCTCCGTTCCTCATCCTGCTGGCGGCGCGTGTTAAACAGCTGTCTGGGGCGAACAGCGGCGAGAAAAGCAAAATCACTATTCCGAGGTTTGCCATCTTGTTCATCGTAGTTGCCATCTTTAACTCGTTCCACCTGTTACCGCAGAGCGTG                                                                                >  SRR19973065.447578/1‑150 (MQ=60)
                                                                 CTCCGTTCCTCATCCTGCTGGCGGCGCGTGTTAAACAGCTGTCTGGGGCGAACAGCGGCGAGAAAAGCAAAATCACTATTCCGAGGTTTGCCATCTTGTTCATCGTAGTTGCCATCTTTAACTCGTTCCACCTGTTACCGCAGAGCGTGG                                                                               <  SRR19973065.610683/150‑1 (MQ=60)
                                                                 CTCCGTTCCTCATCCTGCTGGCGGCGCGTGTTAAACAGCTGTCTGGGGCGAACAGCGGCGAGAAAAGCAAAATCACTATTCCGAGGTTTGCCATCTTGTTCATCGTAGTTGCCATCTTTAACTCGTTCCACCTGTTACCGCAGAGCGTGG                                                                               >  SRR19973065.2547653/1‑150 (MQ=60)
                                                                 CTCCGTTCCTCATCCTGCTGGCGGCGCGTGTTAAACAGCTGTCTGGGGCGAACAGCGGCGAGAAAAGCAAAATCACTATTCCGAGGTTTGCCATCTTGTTCATCGTAGTTGCCATCTTTAACTCGTTCCACCTGTTACCGCAGAGCGTGG                                                                               <  SRR19973065.610213/150‑1 (MQ=60)
                                                                  TCCGTTCCTCATCCTGCTGGCGGCGCGTGTTAAACAGCTGTCTGGGGCGAACAGCGGCGAGAAAAGCAAAATCACTATTCCGAGGTTTGCCATCTTGTTCATCGTAGTTGCCATCTTTAACTCGTTCCACCTGTTACCGCAGAGCGTGGT                                                                              <  SRR19973065.139373/150‑1 (MQ=60)
                                                                     GTTCCTCATCCTGCTGGCGGCGCGTGTTAAACAGCTGTCTGGGGCGAACAGCGGCGAGAAAAGCAAAATCACTATTCCGAGGTTTGCCATCTTGTTCATCGTAGTTGCCATCTTTAACTCGTTCCACCTGTTACCGCAGAGCGTGGTGAA                                                                           >  SRR19973065.2032976/1‑150 (MQ=60)
                                                                     GTTCCTCATCCTGCTGGCGGCGCGTGTTAAACAGCTGTCTGGGGCGAACAGCGGCGAGAAAAGCAAAATCACTATTCCGAGGTTTGCCATCTTGTTCATCGTAGTTGCCATCTTTAACTCGTTCCACCTGTTACCGCAGAGCGTGGTGAA                                                                           >  SRR19973065.2929474/1‑150 (MQ=60)
                                                                     GTTCCTCATCCTGCTGGCGGCGCGTGTTAAACAGCTGTCTGGGGCGAACAGCGGCGAGAAAAGCAAAATCACTATTCCGAGGTTTGCCATCTTGTTCATCGTAGTTGCCATCTTTAACTCGTTCCACCTGTTACCGCAGAGCGTGGTGAA                                                                           >  SRR19973065.505241/1‑150 (MQ=60)
                                                                     GTTCCTCATCCTGCTGGCGGCGCGTGTTAAACAGCTGTCTGGGGCGAACAGCGGCGAGAAAAGCAAAATCACTATTCCGAGGTTTGCCATCTTGTTCATCGTAGTTGCCATCTTTAACTCGTTCCACCTGTTACCGCAGAGCGTGGTGAA                                                                           >  SRR19973065.505292/1‑150 (MQ=60)
                                                                         CTCATCCTGCTGGCGGCGCGTGTTAAACAGCTGTCTGGGGCGAACAGCGGCGAGAAAAGCAAAATCACTATTCCGAGGTTTGCCATCTTGTTCATCGTAGTTGCCATCTTTAACTCGTTCCACCTGTTACCGCAGAGCGTGGTGAACATG                                                                       <  SRR19973065.1526989/150‑1 (MQ=60)
                                                                           CATCCTGCTGGCGGCGCGTGTTAAACAGCTGTCTGGGGCGAACAGCGGCGAGAAAAGCAAAATCACTATTCCGAGGTTTGCCATCTTGTTCATCGTAGTTGCCATCTTTAACTCGTTCCACCTGTTACCGCAGAGCGTGGTGAACATGCT                                                                     >  SRR19973065.1644937/1‑150 (MQ=60)
                                                                            ATCCTGCTGGCGGCGCGTGTTAAACAGCTGTCTGGGGCGAACAGCGGCGAGAAAAGCAAAATCACTATTCCGAGGTTTGCCATCTTGTTCATCGTAGTTGCCATCTTTAACTCGTTCCACCTGTTACCGCAGAGCGTGGTGAACATGCTG                                                                    >  SRR19973065.3224023/1‑150 (MQ=60)
                                                                            ATCCTGCTGGCGGCGCGTGTTAAACAGCTGTCTGGGGCGAACAGCGGCGAGAAAAGCAAAATCACTATTCCGAGGTTTGCCATCTTGTTCATCGTAGTTGCCATCTTTAACTCGTTCCACCTGTTACCGCAGAGCGTGGTGAACATGCTG                                                                    <  SRR19973065.144689/150‑1 (MQ=60)
                                                                                  CTGGCGGCGCGTGTTAAACAGCTGTCTGGGGCGAACAGCGGCGAGAAAAGCAAAATCACTATTCCGAGGTTTGCCATCTTGTTCATCGTAGTTGCCATCTTTAACTCGTTCCACCTGTTACCGCAGAGCGTGGTGAACATGCTGGTAACG                                                              <  SRR19973065.1191354/150‑1 (MQ=60)
                                                                                       GGCGCGTGTTAAACAGCTGTCTGGGGCGAACAGCGGCGAGAAAAGCAAAATCACTATTCCGAGGTTTGCCATCTTGTTCATCGTAGTTGCCATCTTTAACTCGTTCCACCTGTTACCGCAGAGCGTGGTGAACATGCTGGTAACGCTGGA                                                         >  SRR19973065.3156366/1‑150 (MQ=60)
                                                                                         CGCGTGTTAAACAGCTGTCTGGGGCGAACAGCGGCGAGAAAAGCAAAATCACTATTCCGAGGTTTGCCATCTTGTTCATCGTAGTTGCCATCTTTAACTCGTTCCACCTGTTACCGCAGAGCGTGGTGAACATGCTGGTAACGCTGGATA                                                       <  SRR19973065.1485978/150‑1 (MQ=60)
                                                                                            GTGTTAAACAGCTGTCTGGGGCGAACAGCGGCGAGAAAAGCAAAATCACTATTCCGAGGTTTGCCATCTTGTTCATCGTAGTTGCCATCTTTAACTCGTTCCACCTGTTACCGCAGAGCGTGGTGAACATGCTGGTAACGCTGGATACCT                                                    <  SRR19973065.1327165/150‑1 (MQ=60)
                                                                                             TGTTAAACAGCTGTCTGGGGCGAACAGCGGCGAGAAAAGCAAAATCACTATTCCGAGGTTTGCCATCTTGTTCATCGTAGTTGCCATCTTTAACTCGTTCCACCTGTTACCGCAGAGCGTGGTGAACATGCTGGTAACGCTGGATACCTT                                                   <  SRR19973065.1661288/150‑1 (MQ=60)
                                                                                              GTTAAACAGCTGTCTGGGGCGAACAGCGGCGAGAAAAGCAAAATCACTATTCCGAGGTTTGCCATCTTGTTCATCGTAGTTGCCATCTTTAACTCGTTCCACCTGTTACCGCAGAGCGTGGTGAACATGCTGGTAACGCTGGATACCTTC                                                  <  SRR19973065.57910/150‑1 (MQ=60)
                                                                                              GTTAAACAGCTGTCTGGGGCGAACAGCGGCGAGAAAAGCAAAATCACTATTCCGAGGTTTGCCATCTTGTTCATCGTAGTTGCCATCTTTAACTCGTTCCACCTGTTACCGCAGAGCGTGGTGAACATGCTGGTAACCCAGGATACCTTC                                                  >  SRR19973065.3416795/1‑150 (MQ=60)
                                                                                                 AAACAGCTGTCTGGGGCGAACAGCGGCGAGAAAAGCAAAATCACTATTCCGAGGTTTGCCATCTTGTTCATCGTAGTTGCCATCTTTAACTCGTTCCACCTGTTACCGCAGAGCGTGGTGAACATGCTGGTAACGCTGGATACCTTCCTG                                               >  SRR19973065.1172861/1‑150 (MQ=60)
                                                                                                 AAACAGCTGTCTGGGGCGAACAGCGGCGAGAAAAGCAAAATCACTATTCCGAGGTTTGCCATCTTGTTCATCGTAGTTGCCATCTTTAACTCGTTCCACCTGTTACCGCAGAGCGTGGTGAACATGCTGGTAACGCTGGATACCTTCCTG                                               >  SRR19973065.2222209/1‑150 (MQ=60)
                                                                                                   ACAGCTGTCTGGGGCGAACAGCGGCGAGTAAAGCAAAATCACTATTCCGAGGTTTGCCATCTTGTTCAGCGGAGTTGCCATCTTTAACTCGTTCCACCTGTTACCGCAGAGCGTGGTGAACATGATGGTAACGCTGGATACCTTCCTGCT                                             <  SRR19973065.722896/150‑1 (MQ=60)
                                                                                                   ACAGCTGTCTGGGGCGAACAGCGGCGAGAAAAGCAAAATCACTATTCCGAGGTTTGCCATCTTGTTCATCGTAGTTGCCATCTTTAACTCGTTCCACCTGTTACCGCAGAGCGTGGTGAACATGCTGGTAACGCTGGATACCTTCCTGCT                                             >  SRR19973065.2937472/1‑150 (MQ=60)
                                                                                                   ACAGCTGTCTGGGGCGAACAGCGGCGAGAAAAGCAAAATCACTATTCCGAGGTTTGCCATCTTGTTCATCGTAGTTGCCATCTTTAACTCGTTCCACCTGTTACCGCAGAGCGTGGTGAACATGCTGGTAACGCTGGATACCTTCCTGCT                                             >  SRR19973065.2937490/1‑150 (MQ=60)
                                                                                                   ACAGCTGTCTGGGGCGAACAGCGGCGAGAAAAGCAAAATCACTATTCCGAGGTTTGCCATCTTGTTCATCGTAGTTGCCATCTTTAACTCGTTCCACCTGTTACCGCAGAGCGTGGTGAACATGCTGGTAACGCTGGATACCTTCCTGCT                                             <  SRR19973065.722737/150‑1 (MQ=60)
                                                                                                   ACAGCTGTCTGGGGCGAACAGCGGCGAGAAAAGCAAAATCACTATTCCGAGGTTTGCCATCTTGTTCATCGTAGTTGCCATCTTTAACTCGTTCCACCTGTTACCGCAGAGCGTGGTGAACATGCTGGTAACGCTGGATACCTTCCTGCT                                             <  SRR19973065.3338870/150‑1 (MQ=60)
                                                                                                          TCTGGGGCGAACAGCGGCGAGAAAAGCAAAATCACTATTCCGAGGTTTGCCATCTTGTTCATCGTAGTTGCCATCTTTAACTCGTTCCACCTGTTACCGCAGAGCGTGGTGAACATGCTGGTAACGCTGGATACCTTCCTGCTGGCAATG                                      <  SRR19973065.2885486/150‑1 (MQ=60)
                                                                                                          TCTGGGGCGAACAGCGGCGAGAAAAGCAAAATCACTATTCCGAGGTTTGCCATCTTGTTCATCGTAGTTGCCATCTTTAACTCGTTCCACCTGTTACCGCAGAGCGTGGTGAACATGCTGGTAACGCTGGATACCTTCCTGCTGGCAATG                                      <  SRR19973065.1124198/150‑1 (MQ=60)
                                                                                                          TCTGGGGCGAACAGCGGCGAGAAAAGCAAAATCACTATTCCGAGGTTTGCCATCTTGTTCATCGTAGTTGCCATCTTTAACTCGTTCCACCTGTTACCGCAGAGCGTGGTGAACATGCTGGTAACGCTGGATACCTTCCTGCTGGCAATG                                      <  SRR19973065.869151/150‑1 (MQ=60)
                                                                                                          TCTGGGGCGAACAGCGGCGAGAAAAGCAAAATCACTATTCCGAGGTTTGCCATCTTGTTCATCGTAGTTGCCATCTTTAACTCGTTCCACCTGTTACCGCAGAGCGTGGTGAACATGCTGGTAACGCTGGATACCTTCCTGCTGGCAATG                                      <  SRR19973065.1434194/150‑1 (MQ=60)
                                                                                                           CTGGGGCGAACAGCGGCGAGAAAAGCAAAATCACTATTCCGAGGTTTGCCATCTTGTTCATCGTAGTTGCCATCTTTAACTCGTTCCACCTGTTACCGCAGAGCGTGGTGAACATGCTGGTAACGCTGGATACCTTCCTGCTGGCAATGG                                     >  SRR19973065.1978731/1‑150 (MQ=60)
                                                                                                            TGGGGCGAACAGCGGCGAGAAAAGCAAAATCACTATTCCGAGGTTTGCCATCTTGTTCATCGTAGTTGCCATCTTTAACTCGTTCCACCTGTTACCGCAGAGCGTGGTGAACATGCTGGTAACGCTGGATACCTTCCTGCTGGCAATGGC                                    >  SRR19973065.2805885/1‑150 (MQ=60)
                                                                                                            TGGGGCGAACAGCGGCGAGAAAAGCAAAATCACTATTCCGAGGTTTGCCATCTTGTTCATCGTAGTTGCCATCTTTAACTCGTTCCACCTGTTACCGCAGAGCGTGGTGAACATGCTGGTAACGCTGGATACCTTCCTGCTGGCAATGGC                                    <  SRR19973065.128186/150‑1 (MQ=60)
                                                                                                             GGGGCGAACAGCGGCGAGAAAAGCAAAATCACTATTCCGAGGTTTGCCATCTTGTTCATCGTAGTTGCCATCTTTAACTCGTTCCACCTGTTACCGCAGAGCGTGGTGAACATGCTGGTAACGCTGGATACCTTCCTGCTGGCAATGGCG                                   >  SRR19973065.1072783/1‑150 (MQ=60)
                                                                                                             GGGGCGAACAGCGGCGAGAAAAGCAAAATCACTATTCCGAGGTTTGCCATCTTGTTCATCGTAGTTGCCATCTTTAACTCGTTCCACCTGTTACCGCAGAGCGTGGTGAACATGCTGGTAACGCTGGATACCTTCCTGCTGGCAATGGCG                                   >  SRR19973065.2339393/1‑150 (MQ=60)
                                                                                                                GCGAACAGCGGCGAGAAAAGCAAAATCACTATTCCGAGGTTTGCCATCTTGTTCATCGTAGTTGCCATCTTTAACTCGTTCCACCTGTTACCGCAGAGCGTGGTGAACATGCTGGTAACGCTGGATACCTTCCTGCTGGCAATGGCGATG                                <  SRR19973065.1938864/150‑1 (MQ=60)
                                                                                                                  GAACAGCGGCGAGAAAAGCAAAATCACTATTCCGAGGTTTGCCATCTTGTTCATCGTAGTTGCCATCTTTAACTCGTTCCACCTGTTACCGCAGAGCGTGGTGAACATGCTGGTAACGCTGGATACCTTCCTGCTGGCAATGGCGATGGC                              >  SRR19973065.2680168/1‑150 (MQ=60)
                                                                                                                   AACATCGGCGAGAAAAGCAAAATCACTATTCAGAGGTTTGCCATCTTGTTCATCGTAGTTGCCATCTTTAACTCGTTCCACCTGTTACCGCAGAGCGTGGTGAACATTCTGGTAACGCTGGATACCTTCCTGCTGGCAATGGCGATGGCG                             <  SRR19973065.1383399/150‑1 (MQ=60)
                                                                                                                     CAGCGGCGAGAAAAGCAAAATCACTATTCCGAGGTTTGCCATCTTGTTCATCGTAGTTGCCATCTTTAACTCGTTCCACCTGTTACCGCAGAGCGTGGTGAACATGCTGGTAACGCTGGATACCTTCCTGCTGGCAATGGCGATGGCGGC                           <  SRR19973065.3527823/150‑1 (MQ=60)
                                                                                                                        CGGCGAGAAAAGCAAAATCACTATTCCGAGGTTTGCCATCTTGTTCATCGTAGTTGCCATCTTTAACTCGTTCCACCTGTTACCGCAGAGCGTGGTGAACATGCTGGTAACGCTGGATACCTTCCTGCTGGCAATGGCGATGGCGGCGCT                        >  SRR19973065.2344642/1‑150 (MQ=60)
                                                                                                                         GGCGAGAAAAGCAAAATCACTATTCCGAGGTTTGCCATCTTGTTCATCGTAGTTGCCATCTTTAACTCGTTCCACCTGTTACCGCAGAGCGTGGTGAACATGCTGGTAACGCTGGATACCTTCCTGCTGGCAATGGCGATGGCGGCGCTG                       >  SRR19973065.253923/1‑150 (MQ=60)
                                                                                                                          GCGAGAAAAGCAAAATCACTATTCCGAGGTTTGCCATCTTGTTCATCGTAGTTGCCATCTTTAACTCGTTCCACCTGTTACCGCAGAGCGTGGTGAACATGCTGGTAACGCTGGATACCTTCCTGCTGGCAATGGCGATGGCGGCGCTGG                      >  SRR19973065.1648096/1‑150 (MQ=60)
                                                                                                                           CGAGAAAAGCAAAATCACTATTCCGAGGTTTGCCATCTTGTTCATCGTAGTTGCCATCTTTAACTCGTTCCACCTGTTACCGCAGAGCGTGGTGAACATGCTGGTAACGCTGGATACCTTCCTGCTGGCAATGGCGATGGCGGCGCTGGG                     >  SRR19973065.464785/1‑150 (MQ=60)
                                                                                                                           CGAGAAAAGCAAAATCACTATTCCGAGGTTTGCCATCTTGTTCATCGTAGTTGCCATCTTTAACTCGTTCCACCTGTTACCGCAGAGCGTGGTGAACATGCTGGTAACGCTGGATACCTTCCTGCTGGCAATGGCGATGGCGGCGCTGGG                     >  SRR19973065.2534215/1‑150 (MQ=60)
                                                                                                                           CGAGAAAAGCAAAATCACTATTCCGAGGTTTGCCATCTTGTTCATCGTAGTTGCCATCTTTAACTCGTTCCACCTGTTACCGCAGAGCGTGGTGAACATGCTGGTAACGCTGGATACCTTCCTGCTGGCAATGGCGATGGCGGCGCTGGG                     >  SRR19973065.1374702/1‑150 (MQ=60)
                                                                                                                             AGAAAAGCAAAATCACTATTCCGAGGTTTGCCATCTTGTTCATCGTAGTTGCCATCTTTAACTCGTTCCACCTGTTACCGCAGAGCGTGGTGAACATGCTGGTAACGCTGGATACCTTCCTGCTGGCAATGGCGATGGCGGCGCTGGGTC                   >  SRR19973065.939414/1‑150 (MQ=60)
                                                                                                                              GAAAAGCAAAATCACTATTCCGAGGTTTGCCATCTTGTTCATCGTAGTTGCCATCTTTAACTCGTTCCACCTGTTACCGCAGAGCGTGGTGAACATGCTGGTAACGCTGGATACCTTCCTGCTGGCAATGGCGATGGCGGCGCTGGGTCT                  <  SRR19973065.1074868/150‑1 (MQ=60)
                                                                                                                                AAAGCAAAATCACTATTCCGAGGTTTGCCATCTTGTTCATCGTAGTTGCCATCTTTAACTCGTTCCACCTGTTACCGCAGAGCGTGGTGAACATGCTGGTAACGCTGGATACCTTCCTGCTGGCAATGGCGATGGCGGCGCTGGGTCTGA                >  SRR19973065.462412/1‑150 (MQ=60)
                                                                                                                                     AAAATCACTATTCCGAGGTTTGCCATCTTGTTCATCGTAGTTGCCATCTTTAACTCGTTCCACCTGTTACCGCAGAGCGTGGTGAACATGCTGGTAACGCTGGATACCTTCCTGCTGGCAATGGCGATGGCGGCGCTGGGTCTGACCACT           >  SRR19973065.2550783/1‑150 (MQ=60)
                                                                                                                                     AAAATCACTATTCCGAGGTTTGCCATCTTGTTCATCGTAGTTGCCATCTTTAACTCGTTCCACCTGTTACCGCAGAGCGTGGTGAACATGCTGGTAACGCTGGATACCTTCCTGCTGGCAATGGCGATGGCGGCGCTGGGTCTGACCACT           >  SRR19973065.1502554/1‑150 (MQ=60)
                                                                                                                                       AATCACTATTCCGAGGTTTGCCATCTTGTTCATAGTAGTTGCCATCTTTAACTCGTTCCAACTGTTACCGCAGAGCGTGGTGAACATGCTGGTAACGCTGGATACCTTCCTGCTGGCTATGGCGATGGCGGCGCTGGGTCTGACCACTCA         <  SRR19973065.3096937/150‑1 (MQ=60)
                                                                                                                                         TCACTATTCCGAGGTTTGCCATCTTGTTCATCGTAGTTGCCATCTTTAACTCGTTCCACCTGTTACCGCAGAGCGTGGTGAACATGCTGGTAACGCTGGATACCTTCCTGCTGGCAATGGCGATGGCGGCGCTGGGTCTGACCACTCACG       >  SRR19973065.917186/1‑150 (MQ=60)
                                                                                                                                         TCACTATTCCGAGGTTTGCCATCTTGTTCATCGTAGTTGCCATCTTTAACTCGTTCCACCTGTTACCGCAGAGCGTGGTGAACATGCTGGTAACGCTGGATACCTTCCTGCTGGCAATGGCGATGGCGGCGCTGGGTCTGACCACTCACG       >  SRR19973065.2683122/1‑150 (MQ=60)
                                                                                                                                         TCACTATTCCGAGGTTTGCCATCTTGTTCATCGTAGTTGCCATCTTTAACTCGTTCCACCTGTTACCGCAGAGCGTGGTGAACATGCTGGTAACGCTGGATACCTTCCTGCTGGCAATGGCGATGGCGGCGCTGGGTCTGACCACTCACG       <  SRR19973065.3217003/150‑1 (MQ=60)
                                                                                                                                         TCACTATTACGAGGTTTGCCATCTTGTTCATCGTAGTTGCCATCTTAAACTAGTTCCACCTGTTACCGCAGAGCGTGGTGAACATTCTGGTAACGCTGGATACCTTCCAGCTGGCAATGGCGATGGCGGCGCTGGGTCTGACCACTCACG       >  SRR19973065.2683086/1‑150 (MQ=60)
                                                                                                                                            CTATTCCGAGGTTTGCCATCTTGTTCATCGTAGTTGCCATCTTTAACTCGTTCCACCTGTTACCGCAGAGCGTGGTGAACATGCTGGTAACGCTGGATACCTTCCTGCTGGCAATGTCGATGGCGGCGCTGGGTCGGACCACTCACGTCA    >  SRR19973065.685657/1‑150 (MQ=60)
                                                                                                                                            CTATTCCGAGGTTTGCCATCTTGTTCATCGTAGTTGCCATCTTTAACTCGTTCCACCTGTTACCGCAGAGCGTGGTGAACATGCTGGTAACGCTGGATACCTTCCTGCTGGCAATGGCGATGGCGGCGCTGGGTCTGACCACTCACGTCA    <  SRR19973065.3513985/150‑1 (MQ=60)
                                                                                                                                            CTATTCCGAGGTTTGCCATCTTGTTCATCGTAGTTGCCATCTTTAACTCGTTCCACCTGTTACCGCAGAGCGTGGTGAACATGCTGGTAACGCTGGATACCTTCCTGCTGGCAATGGCGATGGCGGCGCTGGGTCTGACCACTCACGTCA    >  SRR19973065.1181044/1‑150 (MQ=60)
                                                                                                                                              ATTCCGAGGTTTGCCATCTTGTTCATCGTAGTTGCCATCTTTAACTCGTTCCACCTGTTACCGCAGAGCGTGGTGAACATGCTGGTAACGCTGGATACCTTCCTGCTGGCAATGGCGATGGCGGCGCTGGGTCTGACCACTCACGTCAGC  <  SRR19973065.1064549/150‑1 (MQ=60)
                                                                                                                                                    |                                                                                                                                               
TGCCATCAGCCCGGATGCGGAAAACGCAGCAGTTATTTCCAAAATGCTGCGCGTGATGATGCTGGCTCCGTTCCTCATCCTGCTGGCGGCGCGTGTTAAACAGCTGTCTGGGGCGAACAGCGGCGAGAAAAGCAAAATCACTATTCCGTGGTTTGCCATCTTGTTCATCGTAGTTGCCATCTTTAACTCGTTCCACCTGTTACCGCAGAGCGTGGTGAACATGCTGGTAACGCTGGATACCTTCCTGCTGGCAATGGCGATGGCGGCGCTGGGTCTGACCACTCACGTCAGC  >  CP009273/2243843‑2244134

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑