Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F434 I0 R1 1130 215.9 7031662 97.1% 6827743 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,831,086 C→A 100% E127* (GAA→TAA)  hydN ← formate dehydrogenase‑H, [4Fe‑4S] ferredoxin subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,831,0860CA96.4% 505.7 / 9.3 165E127* (GAA→TAA) hydNformate dehydrogenase‑H, [4Fe‑4S] ferredoxin subunit
Reads supporting (aligned to +/- strand):  ref base C (3/3);  new base A (84/75);  total (87/78)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 165 total aligned reads displayed.

CATCAGCGCCGTACGGCGGCGTTTTTCTGCGCTCAGTTGTTCAAGTTTATTACGATCGACACAAATCAGCGCATGAGTCGGGCAAGCCGCCATACACGCCGGGCCGTCTTCACGATGGTTGCACAGGTCGCATTTATTGGCTTCGGCTTTGTCAGCCCGTACATTCAGACCCGCGCCGCTGTTGCGGATCACCGGACGTACCACCACTTCCATCGCACCATACGGGCAAGCCACAACGCAGGTTTTGCAACCAATGCAACGTTCCTGCATCACATGAACAAACCCTTTAT  >  CP009273/2830943‑2831232
                                                                                                                                               |                                                                                                                                                  
cATCAGCGCCGTACGGCGGCGTTTTTCTGCGCTCAGTTGTTCAAGTTTATTACGATCGACACAAATCAGCGCATGAGTCGGGCAAGCCGCCATACACGCCGGGCCGTCTTCACGATGGTTGCACAGGTCGCATTTATTGGCTTAGGCt                                                                                                                                                >  2:145713/1‑148 (MQ=255)
   cAGCGCCGTACGGCGGCGTTTTTCTGCGCTCAGTTGTTCAAGTTTATTACGATCGACACAAATCAGCGCAAGAGTCGGGCAAGCCGCCATACACGCCGGGCCGTCTTCACGATGGTTGCACAGGTCGCATTTATTGGCTTAGGCTTTg                                                                                                                                             >  2:745365/1‑148 (MQ=255)
     gcgcCGTACGGCGGCGTTTTTCTGCGCTCAGTTGTTCAAGTTTATTACGATCGACACAAATCAGCGCATGAGTCGGGCAAGCCGCCATACACGCCGGGCCGTCTTCACGATGGTTGCACAGGTCGCATTTATTGGCTTAGGCTTTGTc                                                                                                                                           <  2:238586/148‑1 (MQ=255)
      cgcCGTACGGCGGCGTTTTTCTGCGCTCAGTTGTTCAAGTTTATTACGATCGACACAAATCAGCGCATGAGTCGGGCAAGCCGCCATACACGCCGGGCCGTCTTCACGATGGTTGCACAGGTCGCATTTATTGGCTTAGGCTTTGTCa                                                                                                                                          <  2:1107137/148‑1 (MQ=255)
       gcCGTACGGCGGCGTTTTTCTGCGCTCAGTTGTTCAAGTTTATTACGATCGACACAAATCAGCGCATGAGTCGGGCAAGCCGCCATACACGCCGGGCCGTCTTCACGATGGTTGCACAGGTCGCATTTATTGGCTTAGGCTTTGTCAg                                                                                                                                         <  2:2904602/148‑1 (MQ=255)
              ggcggcGTTTTTCTGCGCTCAGTTGTTCAAGTTTATTACGATCGACACAAATCAGCGCATGAGTCGGGCAAGCCGCCATACACGCCGGGCCGTCTTCACGATGGTTGCACAGGTCGCATTTATTGGCTTAGGCTTTGTCAGCCCGTAc                                                                                                                                  <  2:754704/148‑1 (MQ=255)
                cggcgTTTTTCTGCGCTCAGTTGTTCAAGTTTATTACGATCGACACAAATCAGCGCATGAGTCGGGCAAGCCGCCATACACGCCGGGCCGTCTTCACGATGGTTGCACAGGTCGCATTTATTGGCTTAGGCTTTGTCAGCCCGTACAt                                                                                                                                <  2:939838/148‑1 (MQ=255)
                cggcgTTTTTCTGCGCTCAGTTGTTCAAGTTTATTACGATCGACACAAATCAGCGCATGAGTCGGGCAAGCCGCCATACACGCCGGGCCGTCTTCACGATGGTTGCACAGGTCGCATTTATTGGCTTAGGCTTTGTCAGCCCGTACAt                                                                                                                                <  2:2465410/148‑1 (MQ=255)
                  gcgTTTTTCTGCGCTCAGTTGTTCAAGTTTATTACGATCGACACAAATCAGCGCATGAGTCGGGCAAGCCGCCATACACGCCGGGCCGTCTTCACGATGGTTGCACAGGTCGCATTTATTGGCTTAGGCTTTGTCAGCCCGTACATTc                                                                                                                              <  2:1692110/148‑1 (MQ=255)
                     tttttCTGCGCTCAGTTGTTCAAGTTTATTACGATCGACACAAATCAGCGCATGAGTCGGGCAAGCCGCCATACACGCCGGGCCGTCTTCACGATGGTTGCACAGGTCGCATTTATTGGCTTAGGCTTTGTCAGCCCGTACATTCAGa                                                                                                                           >  1:1053021/1‑148 (MQ=255)
                     tttttCTGCGCTCAGTTGTTCAAGTTTATTACGATCGACACAAATCAGCGCATGAGTCGGGCAAGCCGCCATACACGCCGGGCCGTCTTCACGATGGTTGCACAGGTCGCATTTATTGGCTTAGGCTTTGTCAGCCCGTACATTCAGa                                                                                                                           >  2:3229248/1‑148 (MQ=255)
                              gcTCAGTTGTTCAAGTTTATTACGATCGACACAAATCAGCGCATGAGTCGGGCAAGCCGCCATACACGCCGGGCCGTCTTCACGATGGTTGCACAGGTCGCATTTATTGGCTTAGGCTTTGTCAGCCCGTACATTCAGACCCGcgccg                                                                                                                  <  2:286587/148‑1 (MQ=255)
                               cTCAGTTGTTCAAGTTTATTACGATCGACACAAATCAGCGCATGAGTCGGGCAAGCCGCCATACACGCCGGGCCGTCTTCACGATGGTTGCACAGGTCGCATTTATTGGCTTAGGCTTTGTCAGCCCGTACATTCAGACCCGcgccgc                                                                                                                 >  1:724661/1‑148 (MQ=255)
                                 cAGTTGTTCAAGTTTATTACGATCGACACAAATCAGCGCATGAGTCGGGCAAGCCGCCATACACGCCGGGCCGTCTTCACGATGGTTGCACAGGTCGCATTTATTGGCTTAGGCTTTGTCAGCCCGTACATTCAGACCCGCGCCGCTg                                                                                                               >  2:2248071/1‑148 (MQ=255)
                                 cAGTTGTTCAAGTTTATTACGATCGACACAAATCAGCGCATGAGTCGGGCAAGCCGCCATACACGCCGGGCCGGCGTCACGTTGGTTGCTCAGGTCGCAGTTCTTGGCTTCGGCGTGGGCAGCCCGTCCAGTCGGAgcggcgcggcgg                                                                                                               >  2:2248391/1‑146 (MQ=255)
                                  aGTTGTTCAAGTTTATTACGATCGACACAAATCAGCGCATGAGTCGGGCAAGCCGCCATACACGCCGGGCCGTCTTCACGATGGTTGCACAGGTCGCATTTATTGGCTTAGGCTTTGTCAGCCCGTACATTCAGACCCGCGCCGCTGt                                                                                                              >  1:3251976/1‑148 (MQ=255)
                                   gttgttCAAGTTTATTACGATCGACACAAATCAGCGCATGAGTCGGGCAAGCCGCCATACACGCCGGGCCGTCTTCACGATGGTTGCACAGGTCGCATTTATTGGCTTAGGCTTTGTCAGCCCGTACATTCAGACCCGCGCCGCTGtt                                                                                                             >  2:3335100/1‑148 (MQ=255)
                                   gttgttCAAGTTTATTACGATCGACACAAATCAGCGCATGAGTCGGGCAAGCCGCCATACACGCCGGGCCGTCTTCACGATGGTTGCACAGGTCGCATTTATTGGCTTAGGCTTTGTCAGCCCGTACATTCAGACCCGCGCCGCTGtt                                                                                                             >  1:2126886/1‑148 (MQ=255)
                                     tgttCAAGTTTATTACGATCGACACAAATCAGCGCATGAGTCGGGCAAGCCGCCATACACGCCGGGCCGTCTTCACGATGGTTGCACAGGTCGCATTTATTGGCTTAGGCTTTGTCAGCCCGTACATTCAGACCCGCGCCGCTGTTGc                                                                                                           >  1:347285/1‑148 (MQ=255)
                                       ttCAAGTTTATTACGATCGACACAAATCAGCGCATGAGTCGGGCAAGCCGCCATACACGCCGGGCCGTCTTCACGATGGTTGCACAGGTCGCATTTATTGGCTTAGGCTTTGTCAGCCCGTACATTCAGACCCGCGCCGCTGTTGCgg                                                                                                         >  2:1810948/1‑148 (MQ=255)
                                         cAAGTTTATTACGATCGACACAAATCAGCGCATGAGTCGGGCAAGCCGCCATACACGCCGGGCCGTCTTCACGATGGTTGCACAGGTCGCATTTATTGGCTTAGGCTTTGTCAGCCCGTACATTCAGACCCGCGCCGCTGTTGCGGAt                                                                                                       >  1:629917/1‑148 (MQ=255)
                                           aGTTTATTACGATCGACACAAATCAGCGCATGAGTCGGGCAAGCCGCCATACACGCCGGGCCGTCTTCACGATGGTTGCACAGGTCGCATTTATTGGCTTAGGCTTTGTCAGCCCGTACATTCAGACCCGCGCCGCTGTTGCGGATCa                                                                                                     <  2:2464478/148‑1 (MQ=255)
                                             tttATTACGATCGACACAAATCAGCGCATGAGTCGGGCAAGCCGCCATACACGCCGGGCCGTCTTCACGATGGTTGCACAGGTCGCATTTATTGGCTTAGGCTTTGTCAGCCCGTACATTCAGACCCGCGCCGCTGTTGCGGATCAcc                                                                                                   <  1:1355793/148‑1 (MQ=255)
                                               tattaCGATCGACACAAATCAGCGCATGAGTCGGGCAAGCCGCCATACACGCCGGGCCGTCTTCACGATGGTTGCACAGGTCGCATTTATTGGCTTAGGCTTTGTCAGCCCGTACATTCAGACCCGCGCCGCTGTTGCGGATCACCgg                                                                                                 <  2:3263902/148‑1 (MQ=255)
                                               tattaCGATCGACACAAATCAGCGCATGAGTCGGGCAAGCCGCCATACACGCCGGGCCGTCTTCACGATGGTTGCACAGGTCGCATTTATTGGCTTAGGCTTTGTCAGCCCGTACATTCAGACCCGCGCCGCTGTTGCGGATCACCgg                                                                                                 <  2:3016122/148‑1 (MQ=255)
                                               tattaCGATCGACACAAATCAGCGCATGAGTCGGGCAAGCCGCCATACACGCCGGGCCGTCTTCACGATGGTTGCACAGGTCGCATTTATTGGCTTAGGCTTTGTCAGCCCGTACATTCAGACCCGCGCCGCTGTTGCGGATCACCgg                                                                                                 <  2:3016265/148‑1 (MQ=255)
                                                      aTCGACACAAATCAGCGCATGAGTCGGGCAAGCCGCCATACACGCCGGGCCGTCTTCACGATGGTTGCACAGGTCGCATTTATTGGCTTAGGCTTTGTCAGCCCGTACATTCAGACCCGCGCCGCTGTTGCGGATCACCGGACGTacc                                                                                          >  1:357731/1‑148 (MQ=255)
                                                      aTCGACACAAATCAGCGCATGAGTCGGGCAAGCCGCCATACACGCCGGGCCGTCTTCACGATGGTTGCACAGGTCGCATTTATTGGCTTAGGCTTTGTCAGCCCGTACATTCAGACCCGCGCCGCTGTTGCGGATCACCGGACGTacc                                                                                          >  1:3193247/1‑148 (MQ=255)
                                                      aTCGACACAAATCAGCGCATGAGTCGGGCAAGCCGCCATACACGCCGGGCCGTCTTCACGATGGTTGCACAGGTCGCATTTATTGGCTTAGGCTTTGTCAGCCCGTACATTCAGACCCGCGCCGCTGTTGCGGATCACCGGACGTacc                                                                                          >  1:2660670/1‑148 (MQ=255)
                                                      aTCGACACAAATCAGCGCATGAGTCGGGCAAGCCGCCATACACGCCGGGCCGTCTTCACGATGGTTGCACAGGTCGCATTTATTGGCTTAGGCTTTGTCAGCCCGTACATTCAGACCCGCGCCGCTGTTGCGGATCACCGGACGTacc                                                                                          >  2:2013306/1‑148 (MQ=255)
                                                       tCGACACAAATCAGCGCATGAGTCGGGCAAGCCGCCATACACGCCGGGCCGTCTTCACGATGGTTGCACAGGTCGCATTTATTGGCTTAGGCTTTGTCAGCCCGTACATTCAGACCCGCGCCGCTGTTGCGGATCACCGGACGTacca                                                                                         >  1:2076723/1‑148 (MQ=255)
                                                       tCGACACAAATCAGCGCATGAGTCGGGCAAGCCGCCATACACGCCGGGCCGTCTTCACGATGGTTGCACAGGTCGCATTTATTGGCTTAGGCTTTGTCAGCCCGTACATTCAGACCCGCGCCGCTGTTGCGGATCACCGGACGTacca                                                                                         <  2:1765670/148‑1 (MQ=255)
                                                       tCGACACAAATCAGCGCATGAGTCGGGCAAGCCGCCATACACGCCGGGCCGTCTTCACGATGGTTGCACAGGTCGCATTTATTGGCTTAGGCTTTGTCAGCCCGTACATTCAGACCCGCGCCGCTGTTGCGGATCACCGGACGTacca                                                                                         >  2:3514455/1‑148 (MQ=255)
                                                       tCGACACAAATCAGCGCATGAGTCGGGCAAGCCGCCATACACGCCGGGCCGTCTTCACGATGGTTGCACAGGTCGCATTTATTGGCTTAGGCTTTGTCAGCCCGTACATTCAGACCCGCGCCGCTGTTGCGGATCACCGGACGTacca                                                                                         >  1:2076730/1‑148 (MQ=255)
                                                         gACACAAATCAGCGCATGAGTCGGGCAAGCCGCCATACACGCCGGGCCGTCTTCACGATGGTTGCACAGGTCGCATTTATTGGCTTAGGCTTTGTCAGCCCGTACATTCAGACCCGCGCCGCTGTTGCGGATCACCGGACGTaccacc                                                                                       <  1:502932/148‑1 (MQ=255)
                                                          acacAAATCAGCGCATGAGTCGGGCAAGCCGCCATACACGCCGGGCCGTCTTCACGATGGTTGCACAGGTCGCATTTATTGGCTTAGGCTTTGTCAGCCCGTACATTCAGACCCGCGCCGCTGTTGCGGATCACCGGACGTAccacca                                                                                      >  2:915597/1‑148 (MQ=255)
                                                            acaAATCAGCGCATGAGTCGGGCAAGCCGCCATACACGCCGGGCCGTCTTCACGATGGTTGCACAGGTCGCATTTATTGGCTTAGGCTTTGTCAGCCCGTACATTCAGACCCGCGCCGCTGTTGCGGATCACCGGACGTACCACCACt                                                                                    >  2:2163238/1‑148 (MQ=255)
                                                               aaTCAGCGCATGAGTCGGGCAAGCCGCCATACACGCCGGGCCGTCTTCACGATGGTTGCACAGGTCGCATTTATTGGCTTAGGCTTTGTCAGCCCGTACATTCAGACCCGCGCCGCTGTTGCGGATCACCGGACGTACCACCACTTcc                                                                                 >  2:91774/1‑148 (MQ=255)
                                                                   aGCGCATGAGTCGGGCAAGCCGCCATACACGCCGGGCCGTCTTCACGATGGTTGCACAGGTCGCATTTATTGGCTTAGGCTTTGTCAGCCCGTACATTCAGACCCGCGCCGCTGTTGCGGATCACCGGACGTACCACCACTTCCATCg                                                                             >  2:1035130/1‑148 (MQ=255)
                                                                     cgcATGAGTCGGGCAAGCCGCCATACACGCCGGGCCGTCTTCACGATGGTTGCACAGGTCGCATTTATTGGCTTAGGCTTTGTCAGCCCGTACATTCAGACCCGCGCCGCTGTTGCGGATCACCGGACGTACCACCACTTCCATCGCa                                                                           >  2:1838159/1‑148 (MQ=255)
                                                                         tGAGTCGGGCAAGCCGCCATACACGCCGGGCCGTCTTCACGATGGTTGCACAGGTCGCATTTATTGGCTTAGGCTTTGTCAGCCCGTACATTCAGACCCGCGCCGCTGTTGCGGATCACCGGACGTACCACCACTTCCATCGCACCAt                                                                       >  1:3328385/1‑148 (MQ=255)
                                                                            gTCGGGCAAGCCGCCATACACGCCGTGCCGTCTTAACGATGGTTGCACAGGTCTCAATTAGATGCTTAGCCTATGTAAGCCCGTAAATTCAGACACGCACCGATTTTGCGCAAAACCTCACTTACCCCCAATACCAACGAACCAtcag                                                                    >  2:798981/1‑145 (MQ=255)
                                                                            gTCGGGCAAGCCGCCATACACGCCGGGCCGTCTTCACGATGGTTGCACAGGTCGCATTTATTGGCTTAGGCTTTGTCAGCCCGTACATTCAGACCCGCGCCGCTGTTGCGGATCACCGGACGTACCACCACTTCCATCGCACCATACg                                                                    >  1:3058252/1‑148 (MQ=255)
                                                                             tCGGGCAAGCCGCCATACACGCCGGGCCGTCTTCACGATGGTTGCACAGGTCGCATTTATTGGCTTAGGCTTTGTCAGCCCGTACATTCAGACCCGCGCCGCTGTTGCGGATCACCGGACGTACCACCACTTCCATCGCACCATACgg                                                                   >  1:3035273/1‑148 (MQ=255)
                                                                             tCGGGCAAGCCGCCATACACGCCGGGCCGTCTTCACGATGGTTGCACAGGTCGCATTTATTGGCTTAGGCTTTGTCAGCCCGTACATTCAGACCCGCGCCGCTGTTGCGGATCACCGGACGTACCACCACTTCCATCGCACCATACgg                                                                   >  1:1776669/1‑148 (MQ=255)
                                                                              cGGGCAAGCCGCCATACACGCCGGGCCGTCTTCACGATGGTTGCACAGGTCGCATTTATTGGCTTAGGCTTTGTCAGCCCGTACATTCAGACCCGCGCCGCTGTTGCGGATCACCGGACGTACCACCACTTCCATCGCACCATACggg                                                                  <  1:338513/148‑1 (MQ=255)
                                                                                ggCAAGCCGCCATACACGCCGGGCCGTCTTCACGATGGTTGCACAGGTCGCATTTATTGGCTTAGGCTTTGTCAGCCCGt                                                                                                                                    <  2:255969/80‑1 (MQ=255)
                                                                                ggCAAGCCGCCATACACGCCGGGCCGTCTTCACGATGGTTGCACAGGTCGCATTTATTGGCTTAGGCTTTGTCAGCCCGt                                                                                                                                    >  1:255969/1‑80 (MQ=255)
                                                                                    aGCCGCCATACACGCCGGGCCGTCTTCACGATGGTTGCACAGGTCGCATTTATTGGCTTAGGCTTTGTCAGCCCGTACATTCAGACCCGCGCCGCTGTTGCGGATCACCGGACGTACCACCACTTCCATCGCACCATACGGGCAAGcc                                                            <  2:2560840/148‑1 (MQ=255)
                                                                                     gccgccATACACGCCGGGCCGTCTTCACGATGGTTGCACAGGTCGCATTTATTGGCTTAGGCTTTGTCAGCCCGTACATTCAGACCCGCGCCGCTGTTGCGGATCACCGGACGTACCACCACTTCCATCGCACCATACGGGCAAGCca                                                           >  1:1273575/1‑148 (MQ=255)
                                                                                     gccgccATACACGCCGGGCCGTCTTCACGATGGTTGCACAGGTCGCATTTATTGGCTTAGGCTTTGTCAGCCCGTACATTCAGACCCGCGCCGCTGTTGCGGATCACCGGACGTACCACCACTTCCATCGCACCATACGGGCAAGCca                                                           >  1:3015715/1‑148 (MQ=255)
                                                                                       cgccATACACGCCGGGCCGTCTTCACGATGGTTGCACAGGTCGCATTTATTGGCTTAGGCTTTGTCAGCCCGTACATTCAGACCCGCGCCGCTGTTGCGGATCACCGGACGTACCACCACTTCCATCGCACCATACGGGCAAGCcaca                                                         <  2:2681560/148‑1 (MQ=255)
                                                                                         ccATACACGCCGGGCCGTCTTCACGATGGTTGCACAGGTCGCATTTATTGGCTTAGGCTTTGTCAGCCCGTACATTCAGACCCGCGCCGCGGTTGCGGATCACCGGACGTACCACCACTTCCATCGCACCATACGGGCAAGCCACAAc                                                       >  1:1221953/1‑148 (MQ=255)
                                                                                          cATACACGCCGGGCCGTCTTCACGATGGTTGCACAGGTCGCATTTATTGGCTTAGGCTTTGTCAGCCCGTACATTCAGACCCGCGCCGCTGTTGCGGATCACCGGACGTACCACCACTTCCATCGCACCATACGGGCAAGCCACAACg                                                      >  2:3447071/1‑148 (MQ=255)
                                                                                          cATACACGCCGGGCCGTCTTCACGATGGTTGCACAGGTCGCATTTATTGGCTTAGGCTTTGTCAGCCCGTACATTCAGACCCGCGCCGCTGTTGCGGATCACCGGACGTACCACCACTTCCATCGCACCATACGGGCAAGCCACAACg                                                      >  1:2734572/1‑148 (MQ=255)
                                                                                          cATACACGCCGGGCCGTCTTCACGATGGTTGCACAGGTCGCATTTATTGGCTTAGGCTTTGTCAGCCCGTACATTCAGACCCGCGCCGCTGTTGCGGATCACCGGACGTACCACCACTTCCATCGCACCATACGGGCAAGCCACAACg                                                      <  1:2242560/148‑1 (MQ=255)
                                                                                           aTACACGCCGGGCCGTCTTCACGATGGTTGCACAGGTCGCATTTATTGGCTTAGGCTTTGTCAGCCCGTACATTCAGACCCGCGCCGCTGTTGCGGATCACCGGACGTACCACCACTTCCATCGCACCATACGGGCAAGCCACAACGc                                                     >  1:3040450/1‑148 (MQ=255)
                                                                                            tACACGCCGGGCCGTCTTCACGATGGTTGCACAGGTCGCATTTATTGGCTTAGGCTTTGTCAGCCCGTACATTCAGACCCGCGCCGCTGTTGCGGATCACCGGACGTACCACCACTTCCATCGCACCATACGGGCAAGCCACAACGCa                                                    >  1:185020/1‑148 (MQ=255)
                                                                                              cacGCCGGGCCGTCTTCACGATGGTTGCACAGGTCGCATTTATTGGCTTAGGCTTTGTCAGCCCGTACATTCAGACCCGCGCCGCTGTTGCGGATCACCGGACGTACCACCACTTCCATCGCACCATACGGGCAAGCCACAACGCAgg                                                  <  1:2961000/148‑1 (MQ=255)
                                                                                                cGCCGGGCCGTCTTCACGATGGTTGCACAGGTCGCATTTATTGGCTTAGGCTTTGTCAGCCCGTACATTCAGACCCGCGCCGCTGTTGCGGATCACCGGACGTACCACCACTTCCATCGCACCATACGGGCAAGCCACAACGCAGGtt                                                <  1:1993905/148‑1 (MQ=255)
                                                                                                cGCCGGGCCGTCTTCACGATGGTTGCACAGGTCGCATTTATTGGCTTAGGCTTTGTCAGCCCGTACATTCAGACCCGCGCCGCTGTTGCGGATCACCGGACGTACCACCACTTCCATCGCACCATACGGGCAAGCCACAACGCAGGtt                                                <  2:1754544/148‑1 (MQ=255)
                                                                                                cGCCGGGCCGTCTTCACGATGGTTGCACAGGTCGCATTTATTGGCTTAGGCTTTGTCAGCCCGTACATTCAGACCCGCGCCGCTGTTGCGGATCACCGGACGTACCACCACTTCCATCGCACCATACGGGCAAGCCACAACGCAGGtt                                                >  1:183681/1‑148 (MQ=255)
                                                                                                cGCCGGGCCGTCTTCACGATGGTTGCACAGGTCGCATTTATTGGCTTAGGCTTTGTCAGCCCGTACATTCAGACCCGCGCCGCGGTTGCGGATCACCGGACGTACCACCACTTCCATCGCACCATACGGGCAAGCCACAACGCAGGtt                                                <  2:3036915/148‑1 (MQ=255)
                                                                                                    gggCCGTCTTCACGATGGTTGCACAGGTCGCATTTATTGGCTTAGGCTTTGTCAGCCCGTACATTCAGACCCGCGCCGCTGTTGCGGATCACCGGACGTACCACCACTTCCATCGCACCATACGGGCAAGCCACAACGCAGGTTTTGc                                            <  2:2229847/148‑1 (MQ=255)
                                                                                                     ggCCGTCTTCACGATGGTTGCACAGGTCGCATTTATTGGCTTAGGCTTTGTCAGCCCGTACATTCAGACCCGCGCCGCTGTTGCGGATCACCGGACGTACCACCACTTCCATCGCACCATACGGGCAAGCCACAACGCAGGTTTTGCa                                           >  2:2244833/1‑148 (MQ=255)
                                                                                                     ggCCGTCTTCACGATGGTTGCACAGGTCGCATTTATTGGCTTAGGCTTTGTCAGCCCGTACATTCAGACCCGCGCCGCTGTTGCGGATCACCGGACGTACCACCACTTCCATCGCACCATACGGGCAAGCCACAACGCAGGTTTTGCa                                           >  2:2574500/1‑148 (MQ=255)
                                                                                                     ggCCGTCTTCACGATGGTTGCACAGGTCGCATTTATTGGCTTAGGCTTTGTCAGCCCGTACATTCAGACCCGCGCCGCTGTTGCGGATCACCGGACGTACCACCACTTCCATCGCACCATACGGGCAAGCCACAACGCAGGTTTTGCa                                           <  2:3134748/148‑1 (MQ=255)
                                                                                                     ggCCGTCTTCACGATGGTTGCACAGGTCGCATTTATTGGCTTAGGCTTTGTCAGCCCGTACATTCAGACCCGCGCCGCTGTTGCGGATCACCGGACGTACCACCACTTCCATCGCACCATACGGGCAAGCCACAACGCAGGTTTTGCa                                           <  1:1251265/148‑1 (MQ=255)
                                                                                                       ccGTCTTCACGATGGTTGCACAGGTCGCATTTATTGGCTTAGGCTTTGTCAGCCCGTACATTCAGACCCGCGCCGCTGTTGCGGATCACCGGACGTACCACCACTTCCATCGCACCATACGGGCAAGCCACAACGCAGGTTTTGCAAc                                         >  2:3509854/1‑148 (MQ=255)
                                                                                                         gTCTTCACGATGGTTGCACAGGTCGCATTTATTGGCTTAGGCTTTGTCAGCCCGTACATTCAGACCCGCGCCGCTGTTGCGGATCACCGGACGTACCACCACTTCCATCGCACCATACGGGCAAGCCACAACGCAGGTTTTGCAACCa                                       <  1:314282/148‑1 (MQ=255)
                                                                                                          tCTTCACGATGGTTGCACAGGTCGCATTTATTGGCTTAGGCTTTGTCAGCCCGTACATTCAGACCCGCGCCGCTGTTGCGGATCACCGGACGTACCACCACTTCCATCGCACCATACGGGCAAGCCACAACGCAGGTTTTGCAACCaa                                      >  2:135855/1‑148 (MQ=255)
                                                                                                          tCTTCACGATGGTTGCACAGGTCGCATTTATTGGCTTAGGCTTTGTCAGCCCGTACATTCAGACCCGCGCCGCTGTTGCGGATCACCGGACGTACCACCACTTCCATCGCACCATACGGGCAAGCCACAACGCAGGTTTTGCAACCaa                                      <  2:2336025/148‑1 (MQ=255)
                                                                                                            ttCACGATGGTTGCACAGGTCGCATTTATTGGCTTAGGCTTTGTCAGCCCGTACATTCAGACCCGCGCCGCTGTTGCGGATCACCGGACGTACCACCACTTCCATCGCACCATACGGGCAAGCCACAACGCAGGTTTTGCAACCAATg                                    <  1:3229248/148‑1 (MQ=255)
                                                                                                                 gATGGTTGCACAGGTCGCATTTATTGGCTTCGGCTTTGTCAGCCCGTACATTCAGACCCGCGCCGCTGTTGCGGATCACCGGACGTACCACCACTTCCATCGCACCATACGGGCAAGCCACAACGCAGGTTTTGCAACCAATGCAACg                               <  1:973938/148‑1 (MQ=255)
                                                                                                                 gATGGTTGCACAGGTCGCATTTATTGGCTTAGGCTTTGTCAGCCCGTACATTCAGACCCGCGCCGCTGTTGCGGATCACCGGACGTACCACCACTTCCATCGCACCATACGGGCAAGCCACAACGCAGGTTTTGCAACCAATGCAACg                               <  2:1510876/148‑1 (MQ=255)
                                                                                                                 gATGGTTGCACAGGTCGCATTTATTGGCTTAGGCTTTGTCAGCCCGTACATTCAGACCCGCGCCGCTGTTGCGGATCACCGGACGTACCACCACTTCCATCGCACCATACGGGCAAGCCACAACGCAGGTTTTGCAACCAATGCAACg                               <  2:2123200/148‑1 (MQ=255)
                                                                                                                   tGGTTGCACAGGTCGCATTTATTGGCTTAGGCTTTGTCAGCCCGTACATTCAGACCCGCGCCGCTGTTGCGGATCACCGGACGTACCACCACTTCCATCGCACCATACGGGCAAGCCACAACGCAGGTTTTGCAACCAATGCAACGtt                             <  2:2643892/148‑1 (MQ=255)
                                                                                                                     gTTGCACAGGTCGCATTTATTGGCTTAGGCTTTGTCAGCCCGTACATTCAGACCCGCGCCGCTGTTGCGGATCACCGGACGTACCACCACTTCCATCGCACCATACGGGCAAGCCACAACGCAGGTTTTGCAACCAATGCAACGTTcc                           <  1:2471422/148‑1 (MQ=255)
                                                                                                                     gTTGCACAGGTCGCATTTATTGGCTTAGGCTTTGTCAGCCCGTACATTCAGACCCGCGCCGCTGTTGCGGATCACCGGACGTACCACCACTTCCATCGCACCATACGGGCAAGCCACAACGCAGGTTTTGCAACCAATGCAACGTTcc                           <  1:2471742/148‑1 (MQ=255)
                                                                                                                      ttGCACAGGTCGCATTTATTGGCTTAGGCTTTGTCAGCCCGTACATTCAGACCCGCGCCGCTGTTGCGGATCACCGGACGTACCACCACTTCCATCGCACCATACGGGCAAGCCACAACGCAGGTTTTGCAACCAATGCAACGTTCCt                          >  2:887011/1‑148 (MQ=255)
                                                                                                                      ttGCACAGGTCGCATTTATTGGCTTAGGCTTTGTCAGCCCGTACATTCAGACCCGCGCCGCTGTTGCGGATCACCGGACGTACCACCACTTCCATCGCACCATACGGGCAAGCCACAACGCAGGTTTTGCAACCAATGCAACGTTCCt                          >  2:561345/1‑148 (MQ=255)
                                                                                                                             ggTCGCATTTATTGGCTTAGGCTTTGTCAGCCCGTACATTCAGACCCGCGCCGCTGTTGCGGATCACCGGACGTACCACCACTTCCATCGCACCATACGGGCAAGCCACAACGCAGGTTTTGCAACCAATGCAACGTTCCTGCATcac                   >  2:428839/1‑148 (MQ=255)
                                                                                                                               tCGCATTTATTGGCTTAGGCTTTGTCAGCCCGTACATTCAGACCCGCGCCGCTGTTGCGGATCACCGGACGTACCACCACTTCCATCGCACCATACGGGCAAGCCACAACGCAGGTTTTGCAACCAATGCAACGTTCCTGCATCACAt                 <  2:2672665/148‑1 (MQ=255)
                                                                                                                                 gCATTTATTGGCTTAGGCTTTGTCAGCCCGTACATTCAGACCCGCGCCGCTGTTGCGGATCACCGGACGTACCACCACTTCCATCGCACCATACGGGCAAGCCACAACGCAGGTTTTGCAACCAATGCAACGTTCCTGCATCACATGa               <  1:145713/148‑1 (MQ=255)
                                                                                                                                 gCATTTATTGGCTTAGGCTTTGTCAGCCCGTACATTCAGACCCGCGCCGCTGTTGCGGATCACCGGACGTACCACCACTTCCATCGCACCATACGGGCAAGCCACAACGCAGGTTTTGCAACCAATGCAACGTTCCTGCATCACATGa               >  2:507391/1‑148 (MQ=255)
                                                                                                                                  cATTTATTGGCTTAGGCTTTGTCAGCCCGTACATTCAGACCCGCGCCGCTGTTGCGGATCACCGGACGTACCACCACTTCCATCGCACCATACGGGCAAGCCACAACGCAGGTTTTGCAACCAATGCAACGTTCCTGCATCACATGaa              >  1:3373412/1‑148 (MQ=255)
                                                                                                                                  cATTTATTGGCTTAGGCTTTGTCAGCCCGTACATTCAGACCCGCGCCGCTGTTGCGGATCACCGGACGTACCACCACTTCCATCGCACCATACGGGCAAGCCACAACGCAGGTTTTGCAACCAATGCAACGTTCCTGCATCACATGaa              >  1:1634746/1‑148 (MQ=255)
                                                                                                                                   aTTTATTGGCTTAGGCTTTGTCAGCCCGTACATTCAGACCCGCGCCGCTGTTGCGGATCACCGGACGTACCACCACTTCCATCGCACCATACGGGCAAGCCACAACGCAGGTTTTGCAACCAATGCAACGTTCCTGCATCACATGAAc             >  2:2432012/1‑148 (MQ=255)
                                                                                                                                   aTTTATTGGCTTAGGCTTTGTCAGCCCGTACATTCAGACCCGCGCCGCTGTTGCGGATCACCGGACGTACCACCACTTCCATCGCACCATACGGGCAAGCCACAACGCAGGTTTTGCAACCAATGCAACGTTCCTGCATCACATGAAc             <  1:2574500/148‑1 (MQ=255)
                                                                                                                                   aTTTATTGGCTTAGGCTTTGTCAGCCCGTACATTCAGACCCGCGCCGCTGTTGCGGATCACCGGACGTACCACCACTTCCATCGCACCATACGGGCAAGCCACAACGCAGGTTTTGCAACCAATGCAACGTTCCTGCATCACATGAAc             <  1:2244885/148‑1 (MQ=255)
                                                                                                                                     ttATTGGCTTAGGCTTTGTCAGCCCGTACATTCAGACCCGCGCCGCTGTTGCGGATCACCGGACGTACCACCACTTCCATCGCACCATACGGGCAAGCCACAACGCAGGTTTTGAAACCAATGCAACGTTCCTGCATCACATGAACaa           >  2:1797563/1‑148 (MQ=255)
                                                                                                                                      tATTGGCTTAGGCTTTGTCAGCCCGTACATTCAGACCCGCGCCGCTGTTGCGGATCACCGGACGTACCACCACTTCCATCGCACCATACGGGCAAGCCACAACGCAGGTTTTGCAACCAATGCAACGTTCCTGCATCACATGAACaaa          <  2:2154890/148‑1 (MQ=255)
                                                                                                                                        ttGGCTTAGGCTTTGTCAGCCCGTACATTCAGACCCGCGCCGCTGTTGCGGATCACCGGACGTACCACCACTTCCATCGCACCATACGGGCAAGCCACAACGCAGGTTTTGCAACCAATGCAACGTTCCTGCATCACATGAACAAAcc        <  1:1692786/148‑1 (MQ=255)
                                                                                                                                        ttGGCTTAGGCTTTGTCAGCCCGTACATTCAGACCCGCGCCGCTGTTGCGGATCACCGGACGTACCACCACTTCCATCGCACCATACGGGCAAGCCACAACGCAGGTTTTGCAACCAATGCAACGTTCCTGCATCACATGAACAAAcc        <  2:629917/148‑1 (MQ=255)
                                                                                                                                        ttGGCTTAGGCTTTGTCAGCCCGTACATTCAGACCCGCGCCGCTGTTGCGGATCACCGGACGTACCACCACTTCCATCGCACCATACGGGCAAGCCACAACGCAGGTTTTGCAACCAATGCAACGTTCCTGCATCACATGAACAAAcc        <  1:2248071/148‑1 (MQ=255)
                                                                                                                                        ttGGCTTAGGATTTGTCAGCCCGAACATTCAGACCCGCGCCGCTGTTGCGGATCACCGGACGTACCACCACTTCCATCGCACCATACGGGCAAGCCACAACGCAGGTTTTGCAACCAATGCAACGTTCCTGCATCACATGAACAAAcc        <  1:1011981/148‑1 (MQ=255)
                                                                                                                                         tGGCTTAGGCTTTGTCAGCCCGTACATTCAGACCCGCGCCGCTGTTGCGGATCACCGGACGTACCACCACTTCCATCGCACCATACGGGCAAGCCACAACGCAGGTTTTGCAACCAATGCAACGTTCCTGCATCACATGAACAAAccc       <  2:2408994/148‑1 (MQ=255)
                                                                                                                                          ggCTTAGGCTTTGTCAGCCCGTACATTCAGACCCGCGCCGCTGTTGCGGATCACCGGACGTACCACCACTTCCATCGCACCATACGGGCAAGCCACAACGCAGGTTTTGCAACCAATGCAACGTTCCTGCATCACATGAACAAACCCt      <  1:1810948/148‑1 (MQ=255)
                                                                                                                                           gCTTAGGCTTTGTCAGCCCGTACATTCAGACCCGCGCCGCTGTTGCGGATCACCGGACGTACCACCACTTCCATCGCACCATACGGGCAAGCCACAACGCAGGTTTTGCAACCAATGCAACGTTCCTGCATCACATGAACAAACCCtt     >  2:2347972/1‑148 (MQ=255)
                                                                                                                                              tCGGCTTTGTCAGCCCGTACATTCAGACCCGCGCCGCTGTTGCGGATCACCGGACGTACCACCACTTCCATCGCACCATACGGGCAAGCCACAACGCAGGTTTTGCAACCAATGCAACGTTCCTGCATCACATGAACAAACCCTTTAt  <  1:2179744/148‑1 (MQ=255)
                                                                                                                                               |                                                                                                                                                  
CATCAGCGCCGTACGGCGGCGTTTTTCTGCGCTCAGTTGTTCAAGTTTATTACGATCGACACAAATCAGCGCATGAGTCGGGCAAGCCGCCATACACGCCGGGCCGTCTTCACGATGGTTGCACAGGTCGCATTTATTGGCTTCGGCTTTGTCAGCCCGTACATTCAGACCCGCGCCGCTGTTGCGGATCACCGGACGTACCACCACTTCCATCGCACCATACGGGCAAGCCACAACGCAGGTTTTGCAACCAATGCAACGTTCCTGCATCACATGAACAAACCCTTTAT  >  CP009273/2830943‑2831232

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CGTACGGCGGCGTTTTTCTGCGCTCAGTTGTTCAAGTTTATTACGATCGACACAAATCAGCGCATGAGTCGGGCAAGCCGCCATACACGCCGGGCCGTCTTCACGATGGTTGCACAGGTCGCATTTATTGGCTTCGGCTTTGTCAGCCCGTACATTCAGACCCGCGCCGCTGTTGCGGATCACCGGACGTACCACCACTTCCATCGCACCATACGGGCAAGCCACAACGCAGGTTTTGCAACCAATGCAACGTTCCTGCATCACATGAACAAACCCTTTATCAC  >  CP009273/2830952‑2831235
                                                                                                                                      |                                                                                                                                                     
CGTACGGCGGCGTTTTTCTGCGCTCAGTTGTTCAAGTTTATTACGATCGACACAAATCAGCGCATGAGTCGGGCAAGCCGCCATACACGCCGGGCCGTCTTCACGATGGTTGCACAGGTCGCATTTATTGGCTTAGGCTTTGTCAGCCCG                                                                                                                                        <  SRR19973065.601856/150‑1 (MQ=60)
         GCGTTTTTCTGCGCTCAGTTGTTCAAGTTTATTACGATCGACACAAATCAGCGCATGAGTCGGGCAAGCCGCCATACACGCCGGGCCGTCTTCACGATGGTTGCACAGGTCGCATTTATTGGCTTAGGCTTTGTCAGCCCGTACATTCAG                                                                                                                               <  SRR19973065.319953/150‑1 (MQ=60)
          CGTTTTTCTGCGCTCAGTTGTTCAAGTTTATTACGATCGACACAAATCAGCGCATGAGTCGGGCAAGCCGCCATACACGCCGGGCCGTCTTCACGATGGTTGCACAGGTCGCATTTATTGGCTTAGGCTTTGTCAGCCCGTACATTCAGA                                                                                                                              >  SRR19973065.1060000/1‑150 (MQ=60)
             TTTTCTGCGCTCAGTTGTTCAAGTTTATTACGATCGACACAAATCAGCGCATGAGTCGGGCAAGCCGCCATACACGCCGGGCCGTCTTCACGATGGTTGCACAGGTCGCATTTATTGGCTTAGGCTTTGTCAGCCCGTACATTCAGACCC                                                                                                                           <  SRR19973065.3300667/150‑1 (MQ=60)
                    CGCTCAGTTGTTCAAGTTTATTACGATCGACACAAATCAGCGCATGAGTCGGGCAAGCCGCCATACACGCCGGGCCGTCTTCACGATGGTTGCACAGGTCGCATTTATTGGCTTAGGCTTTGTCAGCCCGTACATTCAGACCCGCGCCGC                                                                                                                    >  SRR19973065.729512/1‑150 (MQ=60)
                       TCAGTTGTTCAAGTTTATTACGATCGACACAAATCAGCGCATGAGTCGGGCAAGCCGCCATACACGCCGGGCCGTCTTCACGATGGTTGCACAGGTCGCATTTATTGGCTTAGGCTTTGTCAGCCCGTACATTCAGACCCGCGCCGCTGT                                                                                                                 >  SRR19973065.3271635/1‑150 (MQ=60)
                       TCAGTTGTTCAAGTTTATTACGATCGACACAAATCAGCGCATGAGTCGGGCAAGCCGCCATACACGCCGGGCCGTCTTCACGATGGTTGCACAGGTCGCATTTATTGGCTTAGGCTTTGTCAGCCCGTACATTCAGACCCGCGCCGCTGT                                                                                                                 >  SRR19973065.1520713/1‑150 (MQ=60)
                        CAGTTGTTCAAGTTTATTACGATCGACACAAATCAGCGCATGAGTCGGGCAAGCCGCCATACACGCCGGGCCGTCTTCACGATGGTTGCACAGGTCGCATTTATTGGCTTAGGCTTTGTCAGCCCGTACATTCAGACCCGCGCCGCTGTT                                                                                                                >  SRR19973065.2140573/1‑150 (MQ=60)
                          GTTGTTCAAGTTTATTACGATCGACACAAATCAGCGCATGAGTCGGGCAAGCCGCCATACACGCCGGGCCGTCTTCACGATGGTTGCACAGGTCGCATTTATTGGCTTAGGCTTTGTCAGCCCGTACATTCAGACCCGCGCCGCTGTTGC                                                                                                              >  SRR19973065.349715/1‑150 (MQ=60)
                          GTTGTTCAAGTTTATTACGATCGACACAAATCAGCGCATGAGTCGGGCAAGCCGCCATACACGCCGGGCCGTCTTCACGATGGTTGCACAGGTCGCATTTATTGGCTTAGGCTTTGTCAGCCCGTACATTCAGACCCGCGCCGCTGTTGC                                                                                                              >  SRR19973065.349704/1‑150 (MQ=60)
                              TTCAAGTTTATTACGATCGACACAAATCAGCGCATGAGTCGGGCAAGCCGCCATACACGCCGGGCCGTCTTCACGATGGTTGCACAGGTCGCATTTATTGGCTTAGGCTTTGTCAGCCCGTACATTCAGACCCGCGCCGCTGTTGCGGAT                                                                                                          >  SRR19973065.634016/1‑150 (MQ=60)
                                CAAGTTTATTACGATCGACACAAATCAGCGCATGAGTCGGGCAAGCCGCCATACACGCCGGGCCGTCTTCACGATGGTTGCACAGGTCGCATTTATTGGCTTAGGCTTTGTCAGCCCGTACATTCAGACCCGCGCCGCTGTTGCGGATCA                                                                                                        <  SRR19973065.3376684/150‑1 (MQ=60)
                                  AGTTTATTACGATCGACACAAATCAGCGCATGAGTCGGGCAAGCCGCCATACACGCCGGGCCGTCTTCACGATGGTTGCACAGGTCGCATTTATTGGCTTAGGCTTTGTCAGCCCGTACATTCAGACCCGCGCCGCTGTTGCGGATCACC                                                                                                      >  SRR19973065.2992582/1‑150 (MQ=60)
                                  AGTTTATTACGATCGACACAAATCAGCGCATGAGTCGGGCAAGCCGCCATACACGCCGGGCCGTCTTCACGATGGTTGCACAGGTCGCATTTATTGGCTTAGGCTTTGTCAGCCCGTACATTCAGACCCGCGCCGCTGTTGCGGATCACC                                                                                                      <  SRR19973065.2932710/150‑1 (MQ=60)
                                   GTTTATTACGATCGACACAAATCAGCGCATGAGTCGGGCAAGCCGCCATACACGCCGGGCCGTCTTCACGATGGTTGCACAGGTCGCATTTATTGGCTTAGGCTTTGTCAGCCCGTACATTCAGACCCGCGCCGCTGTTGCGGATCACCG                                                                                                     <  SRR19973065.1259533/150‑1 (MQ=60)
                                    TTTATTACGATCGACACAAATCAGCGCATGAGTCGGGCAAGCCGCCATACACGCCGGGCCGTCTTCACGATGGTTGCACAGGTCGCATTTATTGGCTTAGGCTTTGTCAGCCCGTACATTCAGACCCGCGCCGCTGTTGCGGATCACCGG                                                                                                    <  SRR19973065.1364779/150‑1 (MQ=60)
                                      TATTACGATCGACACAAATCAGCGCATGAGTCGGGCAAGCCGCCATACACGCCGGGCCGTCTTCACGATGGTTGCACAGGTCGCATTTATTGGCTTAGGCTTTGTCAGCCCGTACATTCAGACCCGCGCCGCTGTTGCGGATCACCGGAC                                                                                                  <  SRR19973065.2037824/150‑1 (MQ=60)
                                           CGATCGACACAAATCAGCGCATGAGTCGGGCAAGCCGCCATACACGCCGGGCCGTCTTCACGATGGTTGCACAGGTCGCATTTATTGGCTTAGGCTTTGTCAGCCCGTACATTCAGACCCGCGCCGCTGTTGCGGATCACCGGACGTACC                                                                                             >  SRR19973065.2677128/1‑150 (MQ=60)
                                           CGATCGACACAAATCAGCGCATGAGTCGGGCAAGCCGCCATACACGCCGGGCCGTCTTCACGATGGTTGCACAGGTCGCATTTATTGGCTTAGGCTTTGTCAGCCCGTACATTCAGACCCGCGCCGCTGTTGCGGATCACCGGACGTACC                                                                                             >  SRR19973065.360221/1‑150 (MQ=60)
                                           CGATCGACACAAATCAGCGCATGAGTCGGGCAAGCCGCCATACACGCCGGGCCGTCTTCACGATGGTTGCACAGGTCGCATTTATTGGCTTAGGCTTTGTCAGCCCGTACATTCAGACCCGCGCCGCTGTTGCGGATCACCGGACGTACC                                                                                             >  SRR19973065.2168749/1‑150 (MQ=60)
                                           CGATCGACACAAATCAGCGCATGAGTCGGGCAAGCCGCCATACACGCCGGGCCGTCTTCACGATGGTTGCACAGGTCGCATTTATTGGCTTAGGCTTTGTCAGCCCGTACATTCAGACCCGCGCCGCTGTTGCGGATCACCGGACGTACC                                                                                             >  SRR19973065.3212531/1‑150 (MQ=60)
                                           CGATCGACACAAATCAGCGCATGAGTCGGGCAAGCCGCCATACACGCCGGGCCGTCTTCACGATGGTTGCACAGGTCGCATTTATTGGCTTAGGCTTTGTCAGCCCGTACATTCAGACCCGCGCCGCTGTTGCGGATCACCGGACGTACC                                                                                             >  SRR19973065.3212503/1‑150 (MQ=60)
                                           CGATCGACACAAATCAGCGCATGAGTCGGGCAAGCCGCCATACACGCCGGGCCGTCTTCACGATGGTTGCACAGGTCGCATTTATTGGCTTAGGCTTTGTCAGCCCGTACATTCAGACCCGCGCCGCTGTTGCGGATCACCGGACGTACC                                                                                             >  SRR19973065.360251/1‑150 (MQ=60)
                                            GATCGACACAAATCAGCGCATGAGTCGGGCAAGCCGCCATACACGCCGGGCCGTCTTCACGATGGTTGCACAGGTCGCATTTATTGGCTTAGGCTTTGTCAGCCCGTACATTCAGACCCGCGCCGCTGTTGCGGATCACCGGACGTACCA                                                                                            >  SRR19973065.2089999/1‑150 (MQ=60)
                                            GATCGACACAAATCAGCGCATGAGTCGGGCAAGCCGCCATACACGCCGGGCCGTCTTCACGATGGTTGCACAGGTCGCATTTATTGGCTTAGGCTTTGTCAGCCCGTACATTCAGACCCGCGCCGCTGTTGCGGATCACCGGACGTACCA                                                                                            >  SRR19973065.2090006/1‑150 (MQ=60)
                                                GACACAAATCAGCGCATGAGTCGGGCAAGCCGCCATACACGCCGGGCCGTCTTCACGATGGTTGCACAGGTCGCATTTATTGGCTTAGGCTTTGTCAGCCCGTACATTCAGACCCGCGCCGCTGTTGCGGATCACCGGACGTACCACCAC                                                                                        <  SRR19973065.506293/150‑1 (MQ=60)
                                                          AGCGCATGAGTCGGGCAAGCCGCCATACACGCCGGGCCGTCTTCACGATGGTTGCACAGGTCGCATTTATTGGCTTAGGCTTTGTCAGCCCGTACATTCAGACCCGCGCCGCTGTTGCGGATCACCGGACGTACCACCACTTCCATCGCA                                                                              >  SRR19973065.2261112/1‑150 (MQ=60)
                                                             GCATGAGTCGGGCAAGCCGCCATACACGCCGGGCCGTCTTCACGATGGTTGCACAGGTCGCATTTATTGGCTTAGGCTTTGTCAGCCCGTACATTCAGACCCGCGCCGCTGTTGCGGATCACCGGACGTACCACCACTTCCATCGCACCA                                                                           >  SRR19973065.1827551/1‑150 (MQ=60)
                                                              CATGAGTCGGGCAAGCCGCCATACACGCCGGGCCGTCTTCACGATGGTTGCACAGGTCGCATTTATTGGCTTAGGCTTTGTCAGCCCGTACATTCAGACCCGCGCCGCTGTTGCGGATCACCGGACGTACCACCACTTCCATCGCACCAT                                                                          >  SRR19973065.3348693/1‑150 (MQ=60)
                                                                 GAGTCGGGCAAGCCGCCATACACGCCGGGCCGTCTTCACGATGGTTGCACAGGTCGCATTTATTGGCTTAGGCTTTGTCAGCCCGTACATTCAGACCCGCGCCGCTGTTGCGGATCACCGGACGTACCACCACTTCCATCGCACCATACG                                                                       >  SRR19973065.3076945/1‑150 (MQ=60)
                                                                 GAGTCGGGCAAGCCGCCATACACGCCGGGCCGTCTTCACGATGGTTGCACAGGTCGCATTTATTGGCTTAGGCTTTGTCAGCCCGTACATTCAGACCCGCGCCGCTGTTGCGGATCACCGGACGTACCACCACTTCCATCGCACCATACG                                                                       >  SRR19973065.1320343/1‑150 (MQ=60)
                                                                  AGTCGGGCAAGCCGCCATACACGCCGGGCCGTCTTCACGATGGTTGCACAGGTCGCATTTATTGGCTTAGGCTTTGTCAGCCCGTACATTCAGACCCGCGCCGCTGTTGCGGATCACCGGACGTACCACCACTTCCATCGCACCATACGG                                                                      >  SRR19973065.3053863/1‑150 (MQ=60)
                                                                  AGTCGGGCAAGCCGCCATACACGCCGGGCCGTCTTCACGATGGTTGCACAGGTCGCATTTATTGGCTTAGGCTTTGTCAGCCCGTACATTCAGACCCGCGCCGCTGTTGCGGATCACCGGACGTACCACCACTTCCATCGCACCATACGG                                                                      >  SRR19973065.1788285/1‑150 (MQ=60)
                                                                     CGGGCAAGCCGCCATACACGCCGGGCCGTCTTCACGATGGTTGCACAGGTCGCATTTATTGGCTTAGGCTTTGTCAGCCCGTACAgatcggaagagcacacgtctgaactccagtcactgatgccagaatctcgggtggggggtgggggg                                                                   >  SRR19973065.257754/1‑85 (MQ=60)
                                                                     CGGGCAAGCCGCCATACACGCCGGGCCGTCTTCACGATGGTTGCACAGGTCGCATTTATTGGCTTAGGCTTTGTCAGCCCGTACATTCAGACCCGCGCCGCTGTTGCGGATCACCGGACGTACCACCACTTCCATCGCACCATACGGGCA                                                                   <  SRR19973065.340875/150‑1 (MQ=60)
                                                                          AAGCCGCCATACACGCCGGGCCGTCTTCACGATGGTTGCACAGGTCGCATTTATTGGCTTAGGCTTTGTCAGCCCGTACATTCAGACCCGCGCCGCTGTTGCGGATCACCGGACGTACCACCACTTCCATCGCACCATACGGGCAAGCCA                                                              >  SRR19973065.3034185/1‑150 (MQ=60)
                                                                          AAGCCGCCATACACGCCGGGCCGTCTTCACGATGGTTGCACAGGTCGCATTTATTGGCTTAGGCTTTGTCAGCCCGTACATTCAGACCCGCGCCGCTGTTGCGGATCACCGGACGTACCACCACTTCCATCGCACCATACGGGCAAGCCA                                                              >  SRR19973065.1281973/1‑150 (MQ=60)
                                                                              CGCCATACACGCCGGGCCGTCTTCACGATGGTTGCACAGGTCGCATTTATTGGCTTAGGCTTTGTCAGCCCGTACATTCAGACCCGCGCCGCGGTTGCGGATCACCGGACGTACCACCACTTCCAACGCACCATACGGGCAAGCCACAAC                                                          >  SRR19973065.1229910/1‑150 (MQ=60)
                                                                               GGCATACACGCCGGGCCGTCTTCACGATGGTTGCACAGGTCGCATTTATTGGCTTAGGCTTTGTCAGCCCGTACATTCAGACCCGCGCCGCTGTTGCGGATCACCGGACGTACCACCACTTCCATCGCACCATACGGGCAAGCCACAACG                                                         >  SRR19973065.2751581/1‑150 (MQ=60)
                                                                                CCATACACGCCGGGCCGTCTTCACGATGGTTGCACAGGTCGCATTTATTGGCTTAGGCTTTGTCAGCCCGTACATTCAGACCCGCGCCGCTGTTGCGGATCACCGGACGTACCACCACTTCCATCGCACCATACGGGCAAGCCACAACGC                                                        >  SRR19973065.3059065/1‑150 (MQ=60)
                                                                                CCATACACGCCGGGCCGTCTTCACGATGGTTGCACAGGTCGCATTTATTGGCTTAGGCTTTGTCAGCCCGTACATTCAGACCCGCGCCGCTGTTGCGGATCACCGGACGTACCACCACTTCCATCGCACCATACGGGCAAGCCACAACGC                                                        >  SRR19973065.3059053/1‑150 (MQ=60)
                                                                                 CATACACGCCGGGCCGTCTTCACGATGGTTGCACAGGTCGCATTTATTGGCTTAGGCTTTGTCAGCCCGTACATTCAGACCCGCGCCGCTGTTGCGGATCACCGGACGTACCACCACTTCCATCGCACCATACGGGCAAGCCACAACGCA                                                       >  SRR19973065.186474/1‑150 (MQ=60)
                                                                                 CATACACGCCGGGCCGTCTTCACGATGGTTGCACAGGTCGCATTTATTGGCTTAGGCTTTGTCAGCCCGTACATTCAGACCCGCGCCGCTGTTGCGGATCACCGGACGTACCACCACTTCCATCGCACCATACGGGCAAGCCACAACGCA                                                       <  SRR19973065.2256806/150‑1 (MQ=60)
                                                                                     CACGCCGGGCCGTCTTCACGATGGTTGCACAGGTCGCATTTATTGGCTTAGGCTTTGTCAGCCCGTACATTCAGACCCGCGCCGCTGTTGCGGATCACCGGACGTACCACCACTTCCATCGCACCATACGGGCAAGCCACAACGCAGGTT                                                   >  SRR19973065.185133/1‑150 (MQ=60)
                                                                                     CACGCCGGGCCGTCTTCACGATGGTTGCACAGGTCGCATTTATTGGCTTAGGCTTTGTCAGCCCGTACATTCAGACCCGCGCCGCTGTTGCGGATCACCGGACGTACCACCACTTCCATCGCACCATACGGGCAAGCCACAACGCAGGTT                                                   <  SRR19973065.2979081/150‑1 (MQ=60)
                                                                                       CGCCGGGCCGTCTTCACGATGGTTGCACAGGTCGCATTTATTGGCTTAGGCTTTGTCAGCCCGTACATTCAGACCCGCGCCGCTGTTGCGGATCACCGGACGTACCACCACTTCCATCGCACCATACGGGCAAGCCACAACGCAGGTTTT                                                 <  SRR19973065.2006828/150‑1 (MQ=60)
                                                                                          CGGGCCGTCTTCACGATGGTTGCACAGGTCGCATTTATTGGCTTAGGCTTTGTCAGCCCGTACATTCAGACCCGCGCCGCTGTTGCGGATCACCGGACGTACCACCACTTCCATCGCACCATACGGGCAAGCCACAACGCAGGTTTTGCA                                              >  SRR19973065.2644658/1‑150 (MQ=60)
                                                                                           GGGCCGTCTTCACGATGGTTGCACAGGTCGCATTTATTGGCTTAGGCTTTGTCAGCCCGTACATTCAGACCCGCGCCGCTGTTGCGGATCACCGGACGTACCACCACTTCCATCGCACCATACGGGCAAGCCACAACGCAGGTTTTGCAA                                             >  SRR19973065.1800372/1‑150 (MQ=60)
                                                                                            GGCCGTCTTCACGATGGTTGCACAGGTCGCATTTATTGGCTTAGGCTTTGTCAGCCCGTACATTCAGACCCGCGCCGCTGTTGCGGATCACCGGACGTACCACCACTTCCATCGCACCATACGGGCAAGCCACAACGCAGGTTTTGCAAC                                            <  SRR19973065.1259503/150‑1 (MQ=60)
                                                                                                GTCTTCACGATGGTTGCACAGGTCGCATTTATTGGCTTAGGCTTTGTCAGCCCGTACATTCAGACCCGCGCCGCTGTTGCGGATCACCGGACGTACCACCACTTCCATCGCACCATACGGGCAAGCCACAACGCAGGTTTTGCAACCAAT                                        <  SRR19973065.316409/150‑1 (MQ=60)
                                                                                                 TCTTCACGATGGTTGCACAGGTCGCATTTATTGGCTTCGGCTTTGTCAGCCCGTACATTCAGACCCGCGCCGCTGTTGCGGATCACCGGACGTACCACCACTTCCATCGCACCATACGGGCAAGCCACAACGCAGGTTTTGCAACCAATG                                       >  SRR19973065.866259/1‑150 (MQ=60)
                                                                                                  CTTCACGATGGTTGCACAGGTCGCATTTATTGGCTTAGGCTTTGTCAGCCCGTACATTCAGACCCGCGCCGCTGTTGCGGATCACCGGACGTACCACCACTTCCATCGCACCATACGGGCAAGCCACAACGCAGGTTTTGCAACCAATGC                                      >  SRR19973065.1969310/1‑150 (MQ=60)
                                                                                                   TTCACGATGGTTGCACAGGTCGCATTTATTGGCTTAGGCTTTGTCAGCCCGTACATTCAGACCCGCGCCGCTGTTGCGGATCACCGGACGTACCACCACTTCCATCGCACCATACGGGCAAGCCACAACGCAGGTTTTGCAACCAATGCA                                     <  SRR19973065.3248631/150‑1 (MQ=60)
                                                                                                        GATGGTTGCACAGGTCGCATTTATTGGCTTCGGCTTTGTCAGCCCGTACATTCAGACCCGCGCCGCTGTTGCGGATCACCGGACGTACCACCACTTCCATCGCACCATACGGGCAAGCCACAACGCAGGTTTTGCAACCAATGCAACGTT                                <  SRR19973065.119728/150‑1 (MQ=60)
                                                                                                        GATGGTTGCACAGGTCGCATTTATTGGCTTCGGCTTTGTCAGCCCGTACATTCAGACCCGCGCCGCTGTTGCGGATCACCGGACGTACCACCACTTCCATCGCACCATACGGGCAAGCCACAACGCAGGTTTTGCAACCAATGCAACGTT                                <  SRR19973065.980408/150‑1 (MQ=60)
                                                                                                            GTTGCACAGGTCGCATTTATTGGCTTAGGCTTTGTCAGCCCGTACATTCAGACCCGCGCCGCTGTTGCGGATCACCGGACGTACCACCACTTCCATCGCACCATACGGGCAAGCCACAACGCAGGTTTTGCAACCAATGCAACGTTCCTG                            <  SRR19973065.2486983/150‑1 (MQ=60)
                                                                                                            GTTGCACAGGTCGCATTTATTGGCTTAGGCTTTGTCAGCCCGTACATTCAGACCCGCGCCGCTGTTGCGGATCACCGGACGTACCACCACTTCCATCGCACCATACGGGCAAGCCACAACGCAGGTTTTGCAACCAATGCAACGTTCCTG                            <  SRR19973065.2486995/150‑1 (MQ=60)
                                                                                                            GTTGCACAGGTCGCATTTATTGGCTTAGGCTTTGTCAGCCCGTACATTCAGACCCGCGCCGCTGTTGCGGATCACCGGACGTACCACCACTTCCATCGCACCATACGGGCAAGCCACAACGCAGGTTTTGCAACCAATGCAACGTTCCTG                            <  SRR19973065.2487305/150‑1 (MQ=60)
                                                                                                                       CGCATTTATTGGCTTAGGCTTTGTCAGCCCGTACATTCAGACCCGCGCCGCTGTTGCGGATCACCGGACGTACCACCACTTCCATCGCACCATACGGGCAAGCCACAACGCAGGTTTTGCAACCAATGCAACGTTCCTGCATCACATGAA                 >  SRR19973065.1645721/1‑150 (MQ=60)
                                                                                                                       CGCATTTATTGGCTTAGGCTTTGTCAGCCCGTACATTCAGACCCGCGCCGCTGTTGCGGATCACCGGACGTACCACCACTTCCATCGCACCATACGGGCAAGCCACAACGCAGGTTTTGCAACCAATGCAACGTTCCTGCATCACATGAA                 >  SRR19973065.3394041/1‑150 (MQ=60)
                                                                                                                        GCATTTATTGGCTTAGGCTTTGTCAGCCCGTACATTCAGACCCGCGCCGCTGTTGCGGATCACCGGACGTACCACCACTTCCATCGCACCATACGGGCAAGCCACAACGCAGGTTTTGCAACCAATGCAACGTTCCTGCATCACATGAAC                <  SRR19973065.146942/150‑1 (MQ=60)
                                                                                                                          ATTTATTGGCTTAGGCTTTGTCAGCCCGTACATTCAGACCCGCGCCGCTGTTGCGGATCACCGGACGTACCACCACTTCCATCGCACCATACGGGCAAGCCACAACGCAGGTTTTGCAACCAATGCAACGTTCCTGCATCACATGAACAA              <  SRR19973065.2589636/150‑1 (MQ=60)
                                                                                                                          ATTTATTGGCTTAGGCTTTGTCAGCCCGTACATTCAGACCCGCGCCGCTGTTGCGGATCACCGGACGTACCACCACTTCCATCGCACCATACGGGCAAGCCACAACGCAGGTTTTGCAACCAATGCAACGTTCCTGCATCACATGAACAA              <  SRR19973065.2259139/150‑1 (MQ=60)
                                                                                                                          ATTTATTGGCTTAGGCTTTGTCAGCCCGTACATTCAGACCCGCGCCGCTGTTGCGGATCACCGGACGTACCACCACTTCCATCGCACCATACGGGCAAGCCACAACGCAGGTTTTGCAACCAATGCAACGTTCCTGCATCACATGAACAA              <  SRR19973065.2590562/150‑1 (MQ=60)
                                                                                                                          ATTTATTGGCTTAGGCTTTGTCAGCCCGTACATTCAGACCCGCGCCGCTGTTGCGGATCACCGGACGTACCACCACTTCCATCGCACCATACGGGCAAGCCACAACGCAGGTTTTGCAACCAATGCAACGTTCCTGCATCACATGAACAA              <  SRR19973065.2259086/150‑1 (MQ=60)
                                                                                                                               TTGGCTTAGGCTTTGTCAGCCCGTACATTCAGACCCGCGCCGCTGTTGCGGATCACCGGACGTACCACCACTTCCATCGCACCATACGGGCAAGCCACAACGCAGGTTTTGCAACCAATGCAACGTTCCTGCATCACATGAACAAACCCT         <  SRR19973065.1704090/150‑1 (MQ=60)
                                                                                                                               TTGGCTTAGGCTTTGTCAGCCCGTACATTCAGACCCGCGCCGCTGTTGCGGATCACCGGACGTACCACCACTTCCATCGCACCATACGGGCAAGCCACAACGCAGGTTTTGCAACCAATGCAACGTTCCTGCATCACATGAACAAACCCT         <  SRR19973065.1703987/150‑1 (MQ=60)
                                                                                                                               TTGGCTTAGGCTTTGTCAGCCCGTACATTCAGACCCGCGCCGCTGTTGCGGATCACCGGACGTACCACCACTTCCATCGCACCATACGGGCAAGCCACAACGCAGGTTTTGCAACCAATGCAACGTTCCTGCATCACATGAACAAACCCT         <  SRR19973065.2262330/150‑1 (MQ=60)
                                                                                                                               TTGGCTTAGGATTTGTCAGCCCGAACATTCAGACCCGCGCCGCTGTTGCGGATCACCGGACGTACCACCACTTCCATCGCACCATACGGGCAAGCCACAACGCAGGTTTTGCAACCAATGCAACGTTCCTGCATCACATGAACAAACCCT         <  SRR19973065.1018692/150‑1 (MQ=60)
                                                                                                                                TGGCTTAGGCTTTGTCAGCCCGTACATTCAGACCCGCGCCGCTGTTGCGGATCACCGGACGTACCACCACTTCCATCGCACCATACGGGCAAGCCACAACGCAGGTTTTGCAACCAATGCAACGTTCCTGCATCACATGAACAAACCCTT        >  SRR19973065.2119412/1‑150 (MQ=60)
                                                                                                                                TGGCTTAGGCTTTGTCAGCCCGTACATTCAGACCCGCGCCGCTGTTGCGGATCACCGGACGTACCACCACTTCCATCGCACCATACGGGCAAGCCACAACGCAGGATTTGCAACCAATGCAACGTTCCTGCATCACATGAACAAACCCTT        >  SRR19973065.3067580/1‑150 (MQ=60)
                                                                                                                                 GGCTTAGGCTTTGTCAGCCCGTACATTCAGACCCGCGCCGCTGTTGCGGATCACCGGACGTACCACCACTTCCATCGCACCATACGGGCAAGCCACAACGCAGGTTTTGCAACCAATGCAACGTTCCTGCATCACATGAACAAACCCTTT       <  SRR19973065.1822863/150‑1 (MQ=60)
                                                                                                                                   CTTAGGCTTTGTCAGCCCGTACATTCAGACCCGCGCCGCTGTTGCGGATCACCGGACGTACCACCACTTCCATCGCACCATACGGGCAAGCCACAACGCAGGTTTTGCAACCAATGCAACGTTCCTGCATCACATGAACAAACCCTTTAT     >  SRR19973065.2997267/1‑150 (MQ=60)
                                                                                                                                    TTAGGCTTTGTCAGCCCGTACATTCAGACCCGCGCCGCTGTTGCGGATCACCGGACGTACCACCACTTCCATCGCACCATACGGGCAAGCCACAACGCAGGTTTTGCAACCAATGCAACGTTCCTGCATCACATGAACAAACCCTTTATC    <  SRR19973065.1164002/150‑1 (MQ=60)
                                                                                                                                     TCGGCTTTGTCAGCCCGTACATTCAGACCCGCGCCGCTGTTGCGGATCACCGGACGTACCACCACTTCCATCGCACCATACGGGCAAGCCACAACGCAGGTTTTGCAACCAATGCAACGTTCCTGCATCACATGAACAAACCCTTTATCA   <  SRR19973065.2193725/150‑1 (MQ=60)
                                                                                                                                     TAGGCTTTGTCAGCCCGTACATTCAGACCCGCGCCGCTGTTGCGGATCACCGGACGTACCACCACTTCCATCGCACCATACGGGCAAGCCACAACGCAGGTTTTGCAACCAATGCAACGTTCCTGCATCACATGAACAAACCCTTTATCA   >  SRR19973065.3444709/1‑150 (MQ=60)
                                                                                                                                     TAGGCTTTGTCAGCCCGTACATTCAGACCCGCGCCGCTGTTGCGGATCACCGGACGTACCACCACTTCCATCGCACCATACGGGCAAGCCACAACGCAGGTTTTGCAACCAATGCAACGTTCCTGCATCACATGAACAAACCCTTTATCA   >  SRR19973065.2913480/1‑150 (MQ=60)
                                                                                                                                     TAGGCTTTGTCAGCCCGTACATTCAGACCCGCGCCGCTGTTGCGGATCACCGGACGTACCACCACTTCCATCGCACCATACGGGCAAGCCACAACGCAGGTTTTGCAACCAATGCAACGTTCCTGCATCACATGAACAAACCCTTTATCA   >  SRR19973065.2913502/1‑150 (MQ=60)
                                                                                                                                      AGGCTTTGTCAGCCCGTACATTCAGACCCGCGCCGCTGTTGCGGATCACCGGACGTACCACCACTTCCATCGCACCATACGGGCAAGCCACAACGCAGGTTTTGCAACCAATGCAACGTTCCTGCATCACATGAACAAACCCTTTATCAC  <  SRR19973065.1284553/150‑1 (MQ=60)
                                                                                                                                      |                                                                                                                                                     
CGTACGGCGGCGTTTTTCTGCGCTCAGTTGTTCAAGTTTATTACGATCGACACAAATCAGCGCATGAGTCGGGCAAGCCGCCATACACGCCGGGCCGTCTTCACGATGGTTGCACAGGTCGCATTTATTGGCTTCGGCTTTGTCAGCCCGTACATTCAGACCCGCGCCGCTGTTGCGGATCACCGGACGTACCACCACTTCCATCGCACCATACGGGCAAGCCACAACGCAGGTTTTGCAACCAATGCAACGTTCCTGCATCACATGAACAAACCCTTTATCAC  >  CP009273/2830952‑2831235

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑