Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F434 I0 R1 1130 215.9 7031662 97.1% 6827743 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,030,592 T→A 100% N292Y (AAT→TAT)  recJ ← ssDNA exonuclease, 5' ‑‑> 3'‑specific

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,030,5920TA99.1% 687.0 / ‑2.6 210N292Y (AAT→TAT) recJssDNA exonuclease, 5' ‑‑> 3'‑specific
Reads supporting (aligned to +/- strand):  ref base T (0/2);  new base A (102/106);  total (102/108)
Fisher's exact test for biased strand distribution p-value = 4.98e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 212 total aligned reads displayed.

GCTTCAATTTGCATTCCTTGTTCGATCTCTTTTCGCGTCTGGTTTAGCGCATCGAGTTCATTTGCCAGCACGCGCGCTTCGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATTGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCATCACGGTTTGCCACTTCAAGCAGCGCTTTAATCCCCGGACGGCACTTTCCGGCTCGGATGCGACTCATCCCCTGCCAGGTCAGAATGCGATT  >  CP009273/3030447‑3030736
                                                                                                                                                 |                                                                                                                                                
gCTTCACTTGGCACTCCTCGTTCGATCTCTTAGCGCGACGCGCTTAGCGCATCGAGTTCATGTGCCAGCACGCGCGCTACGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCACTCGGCCGGCAGCATAga                                                                                                                                                <  2:677292/148‑1 (MQ=255)
gCTTCAATTTGCATTCCTTGTTCGATCTCTTTTCGCGTCTGGTTTAGCGCATCGAGTTCATTTGCCAGCACGCGCGCTTCGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATAga                                                                                                                                                >  1:1284559/1‑148 (MQ=255)
 cTTCAATTTGCATTCCTTGTTCGATCTCTTTTCGCGTCTGGTTTAGCGCATCGAGTTCATTTGCCAGCACGCGCGCTTCGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATagag                                                                                                                                               <  2:3175365/148‑1 (MQ=255)
 cTTCAATTTGCATTCCTTGTTCGATCTCTTTTCGCGTCTGGTTTAGCGCATCGAGTTCATTTGCCAGCACGCGCGCTTCGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATagag                                                                                                                                               <  2:2200948/148‑1 (MQ=255)
  ttCAATTTGCATTCCTTGTTCGATCTCTTTTCGCGTCTGGTTTAGCGCATCGAGTTCATTTGCCAGCACGCGCGCTTCGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATAgaga                                                                                                                                              <  1:70234/148‑1 (MQ=255)
  ttCAATTTGCATTCCTTGTTCGATCTCTTTTCGCGTCTGGTTTAGCGCATCGAGTTCATTTGCCAGCACGCGCGCTTCGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATAgaga                                                                                                                                              <  1:2549371/148‑1 (MQ=255)
  ttCAATTTGCATTCCTTGTTCGATCTCTTTTCGCGTCTGGTTTAGCGCATCGAGTTCATTTGCCAGCACGCGCGCTTCGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATAgaga                                                                                                                                              <  1:3130424/148‑1 (MQ=255)
   tCAATTTGCATTCCTTGTTCGATCTCTTTTCGCGTCTGGTTTAGCGCATCGAGTTCATTTGCCAGCACGCGCGCTTCGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATAGAGAc                                                                                                                                             >  1:2512464/1‑148 (MQ=255)
   tCAATTTGCATTCCTTGTTCGATCTCTTTTCGCGTCTGGTTTAGCGCATCGAGTTCATTTGCCAGCACGCGCGCTTCGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATAGAGAc                                                                                                                                             >  2:3319035/1‑148 (MQ=255)
   tCAATTTGCATTCCTTGTTCGATCTCTTTTCGCGTCTGGTTTAGCGCATCGAGTTCATTTGCCAGCACGCGCGCTTCGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATAGAGAc                                                                                                                                             >  1:751814/1‑148 (MQ=255)
    cAATTTGCATTCCTTGTTCGATCTCTTTTCGCGTCTGGTTTAGCGCATCGAGTTCATTTGCCAGCACGCGCGCTTCGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATAGAGACg                                                                                                                                            <  1:1579266/148‑1 (MQ=255)
    cAATTTGCATTCCTTGTTCGATCTCTTTTCGCGTCTGGTTTAGCGCATCGAGTTCATTTGCCAGCACGCGCGCTTCGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATAGAGACg                                                                                                                                            <  2:657610/148‑1 (MQ=255)
     aaTTTGCATTCCTTGTTCGATCTCTTTTCGCGTCTGGTTTAGCGCATCGAGTTCATTTGCCAGCACGCGCGCTTCGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATAGAGACGt                                                                                                                                           >  1:1382693/1‑148 (MQ=255)
        ttGCATTCCTTGTTCGATCTCTTTTCGCGTCTGGTTTAGCGCATCGAGTTCATTTGCCAGCACGCGCGCTTCGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATAGAGACGTGGc                                                                                                                                        >  2:308780/1‑148 (MQ=255)
         tGCATTCCTTGTTCGATCTCTTTTCGCGTCTGGTTTAGCGCATCGAGTTCATTTGCCAGCACGCGCGCTTCGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATAGAGACGTGGcc                                                                                                                                       <  1:543093/148‑1 (MQ=255)
           cATTCCTTGTTCGATCTCTTTTCGCGTCTGGTTTAGCGCATCGAGTTCATTTGCCAGCACGCGCGCTTCGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATAGAGACGTGGcccc                                                                                                                                     <  2:1852774/148‑1 (MQ=255)
           cATTCCTTGTTCGATCTCTTTTCGCGTCTGGTTTAGCGCATCGAGTTCATTTGCCAGCACGCGCGCTTCGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATAGAGACGTGGcccc                                                                                                                                     >  1:390548/1‑148 (MQ=255)
                    ttCGATCTCTTTTCGCGTCTGGTTTAGCGCATCGAGTTCATTTGCCAGCACGCGCGCTTCGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATAGAGACGTGGCCCCAGCGCaaaa                                                                                                                            >  1:1431625/1‑148 (MQ=255)
                        aTCTCTTTTCGCGTCTGGTTTAGCGCATCGAGTTCATTTGCCAGCACGCGCGCTTCGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATAGAGACGTGGCCCCAGCGCAAAACCTa                                                                                                                        >  1:3273401/1‑148 (MQ=255)
                           tctTTTCGCGTCTGGTTTAGCGCATCGAGTTCATTTGCCAGCACGCGCGCTTCGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCTGGCAGCATAGAGACGTGGCCCCAGCGCAAAACCTAAAt                                                                                                                     >  2:2790789/1‑148 (MQ=255)
                           tctTTTCGCGTCTGGTTTAGCGCATCGAGTTCATTTGCCAGCACGCGCGCTTCGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATAGAGACGTGGCCCCAGCGCAAAACCTAAAt                                                                                                                     >  1:2653330/1‑148 (MQ=255)
                            ctTTTCGCGTCTGGTTTAGCGCATCGAGTTCATTTGCCAGCACGCGCGCTTCGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATAGAGACGTGGCCCCAGCGCAAAACCTAAATc                                                                                                                    >  2:1614509/1‑148 (MQ=255)
                             ttttCGCGTCTGGTTTAGCGCATCGAGTTCATTTGCCAGCACGCGCGCTTCGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATAGAGACGTGGCCCCAGCGCAAAACCTAAATCg                                                                                                                   >  2:1111784/1‑148 (MQ=255)
                              tttCGCGTCTGGTTTAGCGCATCGAGTTCATTTGCCAGCACGCGCGCTTCGCGGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATAGAGACGTGGCCCCAGCGCAAAACCTAACTCGc                                                                                                                  <  1:649334/148‑1 (MQ=255)
                              tttCGCGTCTGGTTTAGCGCATCGAGTTCATTTGCCAGCACGCGCGCTTCGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATAGAGACGTGGCCCCAGCGCAAAACCTAAATCGc                                                                                                                  >  1:1098056/1‑148 (MQ=255)
                               ttCGCGTCTGGTTTAGCGCATCGAGTTCATTTGCCAGCACGCGCGCTTCGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATAGAGACGTGGCCCCAGCGCAAAACCTAAATCGCt                                                                                                                 <  2:1036908/148‑1 (MQ=255)
                                   cgTCTGGTTTAGCGCATCGAGTTCATTTGCCAGCACGCGCGCTTCGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATAGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTggcg                                                                                                             >  1:124417/1‑148 (MQ=255)
                                    gTCTGGTTTAGCGCATCGAGTTCATTTGCCAGCACGCGCGCTTCGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATAGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTggcgg                                                                                                            <  1:2240965/148‑1 (MQ=255)
                                    gTCTGGTTTAGCGCATCGAGTTCATTTGCCAGCACGCGCGCTTCGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATAGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTggcgg                                                                                                            <  1:2240496/148‑1 (MQ=255)
                                      cTGGTTTAGCGCATCGAGTTCATTTGCCAGCACGCGCGCTTCGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATAGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGgcggcg                                                                                                          <  1:2022238/148‑1 (MQ=255)
                                      cTGGTTTAGCGCATCGAGTTCATTTGCCAGCACGCGCGCTTCGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATAGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGgcggcg                                                                                                          >  2:3235602/1‑148 (MQ=255)
                                        ggTTTAGCGCATCGAGTTCATTTGCCAGCACGCGCGCTTCGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATAGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAg                                                                                                        <  1:1881416/148‑1 (MQ=255)
                                         gTTTAGCGCATCGATTTCATTTGCCAGCACGCGCGCTTCGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATATAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGt                                                                                                       >  2:1399987/1‑148 (MQ=255)
                                         gTTTAGCGCATCGAGTTCATTTGCCAGCACGCGCGCTTCGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATAGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGt                                                                                                       <  2:745239/148‑1 (MQ=255)
                                         gTTTAGCGCATCGAGTTCATTTGCCAGCACGCGCGCTTCGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATAGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGt                                                                                                       >  2:111903/1‑148 (MQ=255)
                                         gTTTAGCGCATCGAGTTCATTTGCCAGCACGCGCGCTTCGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATAGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGAGAGt                                                                                                       >  2:1632560/1‑148 (MQ=255)
                                           ttAGCGCATCGAGTTCATTTGCCAGCACGCGCGCTTCGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATAGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGttt                                                                                                     >  1:624965/1‑148 (MQ=255)
                                           ttAGCGCATCGAGTTCATTTGCCAGCACGCGCGCTTCGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATAGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGttt                                                                                                     <  1:150493/148‑1 (MQ=255)
                                            tAGCGCATCGAGTTCATTTGCCAGCACGCGCGCTTCGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATAGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGtttt                                                                                                    >  2:2494920/1‑148 (MQ=255)
                                                   tCGAGTTCATTTGCCAGCACGCGCGCTTCGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATAGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCAt                                                                                             >  2:1139323/1‑148 (MQ=255)
                                                    cGAGTTCATTTGCCAGCACGCGCGCTTCGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATAGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCATc                                                                                            <  1:1261873/148‑1 (MQ=255)
                                                    cGAGTTCATTTGCCAGCACGCGCGCTTCGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATAGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCATc                                                                                            <  1:1667637/148‑1 (MQ=255)
                                                         tCATTTGCCAGCACGCGCGCTTCGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATAGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCATCACGGt                                                                                       >  2:3457906/1‑148 (MQ=255)
                                                          cATTTGCCAGCACGCGCGCTTCGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATAGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCATCACGGtt                                                                                      <  2:1073375/148‑1 (MQ=255)
                                                            tttGCCAGCACGCGCGCTTCGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATAGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCATCACGGTTTg                                                                                    <  1:893944/148‑1 (MQ=255)
                                                            tttGCCAGCACGCGCGCTTCGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATAGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCATCACGGTTTg                                                                                    >  2:2898180/1‑148 (MQ=255)
                                                            tttGCCAGCACGCGCGCTTCGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATAGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCATCACGGTTTg                                                                                    <  1:2896032/148‑1 (MQ=255)
                                                             ttGCCAGCACGCGCGCTTCGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATAGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCATCACGGTTTGc                                                                                   <  2:843048/148‑1 (MQ=255)
                                                             ttGCCAGCACGCGCGCTTCGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATAGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCATCACGGTTTGc                                                                                   <  1:3183920/148‑1 (MQ=255)
                                                               gCCAGCACGCGCGCTTCGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATAGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCATCACGGTTTGCCa                                                                                 <  1:170196/148‑1 (MQ=255)
                                                                ccAGCACGCGCGCTTCGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATAGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCATCACGGTTTGCCAc                                                                                <  1:3509078/148‑1 (MQ=255)
                                                                ccAGCACGCGCGCTTCGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATAGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCATCACGGTTTGCCAc                                                                                <  1:1773705/148‑1 (MQ=255)
                                                                  aGCACGCGCGCTTCGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATAGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCATCACGGTTTGCCACtt                                                                              <  2:2632710/148‑1 (MQ=255)
                                                                  aGCACGCGCGCTTCGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATAGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCATCACGGTTTGCCACtt                                                                              <  2:779607/148‑1 (MQ=255)
                                                                  aGCACGCGCGCTTCGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATAGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCATCACGGTTTGCCACtt                                                                              <  1:776021/148‑1 (MQ=255)
                                                                      cgcgcgCTTCGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATAGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCATCACGGTTTGCCACTTCAag                                                                          <  2:1287121/148‑1 (MQ=255)
                                                                         gcgcTTCGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATAGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCATCACGGTTTGCCACTTCAagcag                                                                       <  2:3510926/148‑1 (MQ=255)
                                                                             ttCGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATAGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCATCACGGTTTGCCACTTCAAGCAGCGCt                                                                   <  1:2962477/148‑1 (MQ=255)
                                                                             ttCGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATAGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCATCACGGTTTGCCACTTCAAGCAGCGCt                                                                   >  2:2379161/1‑148 (MQ=255)
                                                                              tCGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATAGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCATCACGGTTTGCCACTTCAAGCAGCGCtt                                                                  <  1:227617/148‑1 (MQ=255)
                                                                              tCGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATAGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCATCACGGTTTGCCACTTCAAGCAGCGCtt                                                                  <  1:1730137/148‑1 (MQ=255)
                                                                                gCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATAGAGACGTGGCCCCAGCGCAAAACCTACATCGCTGGCGGCGAGTTTTTGTGCATCACGGTTTGCCACTTCAAGCAGCGCTTTa                                                                <  2:3116180/148‑1 (MQ=255)
                                                                                     tgttgtCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATAGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCATCACGGTTTGCCACTTCAAGCAGCGCTTTAATccc                                                           >  1:1233016/1‑148 (MQ=255)
                                                                                     tgttgtCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATAGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCATCACGGTTTGCCACTTCAAGCAGCGCTTTAATccc                                                           >  1:26874/1‑148 (MQ=255)
                                                                                      gttgtCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATAGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCATCACGGTTTGCCACTTCAAGCAGCGCTTTAATcccc                                                          >  1:3455362/1‑148 (MQ=255)
                                                                                       ttgtCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATAGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCATCACGGTTTGCCACTTCAAGCAGCGCTTTAATCCCCg                                                         >  2:1257859/1‑148 (MQ=255)
                                                                                        tgtCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATAGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCATCACGGTTTGCCACTTCAAGCAGCGCTTTAATCCCCgg                                                        >  1:1021677/1‑148 (MQ=255)
                                                                                          tCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATAGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCATCACGGTTTGCCACTTCAAGCAGCGCTTTAATCCCCGGAc                                                      >  1:2559125/1‑148 (MQ=255)
                                                                                            gCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATTGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCATCACGGTTTGCCACTTCAAGCAGCGCTTTAATCCCCGGACgg                                                    <  2:2764796/148‑1 (MQ=255)
                                                                                               caacaacaGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATAGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCATCACGGTTTGCCACTTCAAGCAGCGCTTTAATCCCCGGACGGCAc                                                 >  1:708462/1‑148 (MQ=255)
                                                                                               caacaacaGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATAGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCATCACGGTTTGCCACTTCAAGCAGCGCTTTAATCCCCGGACGGCAc                                                 >  2:1552225/1‑148 (MQ=255)
                                                                                                aacaacaGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATAGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCATCACGGTTTGCCACTTCAAGCAGCGCTTTAATCCCCGGACGGCACt                                                <  2:707912/148‑1 (MQ=255)
                                                                                                 acaacaGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATAGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCATCACGGTTTGCCACTTCAAGCAGCGCTTTAATCCCCGGACGGCACtt                                               <  1:3319035/148‑1 (MQ=255)
                                                                                                  caacaGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATAGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCATCACGGTTTGCCACTTCAAGCAGCGCTTTAATCCCCGGACGACACttt                                              <  2:800895/148‑1 (MQ=255)
                                                                                                   aacaGCGCCACACCTACGGACATATCGTCCAGTCGTCCGGCAGCATAGAGACGTGGCCCCAGCGCAAAACCTAACTCGCTGGCGGCGAGATTTTGTGCATCACGGTTTGCCACTTCAAGCAGCGCTTTAATCCCCGGACGGCACTTTc                                             <  2:2369434/148‑1 (MQ=255)
                                                                                                   aacaGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATAGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCATCACGGTTTGCCACTTCAAGCAGCGCTTTAATCCCCGGACGGCACTTTc                                             >  2:62136/1‑148 (MQ=255)
                                                                                                   aacaGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATAGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCATCACGGTTTGCCACTTCAAGCAGCGCTTTAATCCCCGGACGGCACTTTc                                             <  2:2533872/148‑1 (MQ=255)
                                                                                                     caGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATAGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCATCACGGTTTGCCACTTCAAGCAGCGCTTTAATCCCCGGACGGCACTTTCCg                                           <  1:2284305/148‑1 (MQ=255)
                                                                                                     caGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATAGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCATCACGGTTTGCCACTTCAAGCAGCGCTTTAATCCCCGGACGGCACTTTCCg                                           >  2:2806037/1‑148 (MQ=255)
                                                                                                     caGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATAGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCATCACGGTTTGCCACTTCAAGCAGCGCTTTAATCCCCGGACGGCACTTTCCg                                           >  2:2971633/1‑148 (MQ=255)
                                                                                                           cacaCCGACGGACATATCGTCCAGTCGTCCGGCAGCATAGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCATCACGGTTTGCCACTTCAAGCAGCGCTTTAATCCCCGGACGGCACTTTCCGGCTCgg                                     <  2:1982043/148‑1 (MQ=255)
                                                                                                            acacCGACGGACATATCGTCCAGTCGTCCGGCAGCATAGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTTCATCACGGTTTGCCACTTCAAGCAGCGCTTTAATCCCCGGACGGCACTTTCCGGCTCGGa                                    >  1:3036547/1‑148 (MQ=255)
                                                                                                             cacCGACGGACATATCGTCCAGTCGTCCGGCAGCATAGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCATCACGGTTTGCCACTTCAAGCAGCGCTTTAATCCCGGGGGGGGGGGGTCCGGCTCGGAt                                   >  2:1606955/1‑148 (MQ=255)
                                                                                                             cacCGACGGACATATCGTCCAGTCGTCCGGCAGCATAGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCATCACGGTTTGCCACTTCAAGCAGCGCTTTAATCCCCGGACGGCACTTTCCGGCTCGGAt                                   >  1:2325436/1‑148 (MQ=255)
                                                                                                             cacCGACGGACATATCGTCCAGTCGTCCGGCAGCATAGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCATCACGGTTTGCCACTTCAAGCAGCGCGGGGGGGGGGGGTCGGCACTTTCCGGCTCGGAt                                   >  2:3347249/1‑148 (MQ=255)
                                                                                                                 gACGGACATATCGTCCAGTCGTCCGGCAGCATAGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCATCACGGTTTGCCACTTCAAGCAGCGCTTTAATCCCCGGACGGCACTTTCCGGCTCGGATGCGa                               <  1:2390534/148‑1 (MQ=255)
                                                                                                                      aCATATCGTCCAGTCGTCCGGCAGCATAGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCATCACGGTTTGCCACTTCAAGCAGCGCTTTAATCCCCGGACGGCACTTTCCGGCTCGGATGCGACTCAt                          <  2:1507305/148‑1 (MQ=255)
                                                                                                                       cATATCGTCCAGTCGTCCGGCAGCATAGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCATCACGGTTTGCCACTTCAAGCAGCGCTTTAATCCCCGGACGGCACTTTCCGGCTCGGATGCGACTCATc                         <  1:3378730/148‑1 (MQ=255)
                                                                                                                        atatCGTCCAGTCGTCCGGCAGCATAGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCATCACGGTTTGCCACTTCAAGCAGCGCTTTAATCCCCGGACGGCACTTTCCGGCTCGGATGCGACTCATcc                        >  2:2226241/1‑148 (MQ=255)
                                                                                                                        atatCGTCCAGTCGTCCGGCAGCATAGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCATCACGGTTTGCCACTTCAAGCAGCGCTTTAATCCCCGGACGGCACTTTCCGGCTCGGATGCGACTCATcc                        >  2:517590/1‑148 (MQ=255)
                                                                                                                        atatCGTCCAGTCGTCCGGCAGCATAGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCATCACGGTTTGCCACTTCAAGCAGCGCTTTAATCCCCGGACGGCACTTTCCGGCTCGGATGCGACTCATcc                        >  2:518069/1‑148 (MQ=255)
                                                                                                                          atCGTCCAGTCGTCCGGCAGCATAGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCATCACGGTTTGCCACTTCAAGCAGCGCTTTAATCCCCGGACGGCACTTTCCGGCTCGGATGCGACTCATcccc                      <  2:405763/148‑1 (MQ=255)
                                                                                                                          atCGTCCAGTCGTCCGGCAGCATAGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCATCACGGTTTGCCACTTCAAGCAGCGCTTTAATCCCCGGACGGCACTTTCCGGCTCGGATGCGACTCATcccc                      >  1:2136836/1‑148 (MQ=255)
                                                                                                                                 aGTCGTCCGGCAGCATAGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCATCACGGTTTGCCACTTCAAGCAGCGCTTTAATCCCCGGACGGCACTTTCCGGCTCGGATGCGACTCATCCCCTGCCAgg               >  2:3379226/1‑148 (MQ=255)
                                                                                                                                 aGTCGTCCGGCAGCATAGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCATCACGGTTTGCCACTTCAAGCAGCGCTTTAATCCCCGGACGGCACTTTCCGGCTCGGATGCGACTCATCCCCTGCCAgg               >  2:3262759/1‑148 (MQ=255)
                                                                                                                                   tcgtcCGGCAGCATAGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCATCACGGTTTGCCACTTCAAGCAGCGCTTTAATCCCCGGACGGCACTTTCCGGCTCGGATGCGACTCATCCCCTGCCAGGTc             <  1:3352885/148‑1 (MQ=255)
                                                                                                                                     gtcCGGCAGCATAGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCATCACGGTTTGCCACTTCAAGCAGCGCTTTAATCCCCGGACGGCACTTTCCGGCTCGGATGCGACTCATCCCCTGCCAGGTCAg           <  2:1990672/148‑1 (MQ=255)
                                                                                                                                      tcCGGCAGCATAGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCATCACGGTTTGCCACTTCAAGCAGCGCTTTAATCCCCGGACGGCACTTTCCGGCTCGGATGCGACTCATCCCCTGCCAGGTCAGa          <  1:1614509/148‑1 (MQ=255)
                                                                                                                                      tcCGGCAGCATAGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCATCACGGTTTGCCACTTCAAGCAGCGCTTTAATCCCCGGACGGCACTTTCCGGCTCGGATGCGACTCATCCCCTGCCAGGTCAGa          <  1:1111784/148‑1 (MQ=255)
                                                                                                                                        cGGCAGCATAGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCATCACGGTTTGCCACTTCAAGCAGCGCTTTAATCCCCGGACGGCACTTTCCGGCTCGGATGCGACTCATCCCCTGCCAGGTCAGAAt        <  1:539879/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
GCTTCAATTTGCATTCCTTGTTCGATCTCTTTTCGCGTCTGGTTTAGCGCATCGAGTTCATTTGCCAGCACGCGCGCTTCGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATTGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCATCACGGTTTGCCACTTCAAGCAGCGCTTTAATCCCCGGACGGCACTTTCCGGCTCGGATGCGACTCATCCCCTGCCAGGTCAGAATGCGATT  >  CP009273/3030447‑3030736

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
AGGGCTTCAATTTGCATTCCTTGTTCGATCTCTTTTCGCGTCTGGTTTAGCGCATCGAGTTCATTTGCCAGCACGCGCGCTTCGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATTGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCATCACGGTTTGCCACTTCAAGCAGCGCTTTAATCCCCGGACGGCACTTTCCGGCTCGGATGCGACTCATCCCCTGCCAGGTCAGAATGCGATTATTAG  >  CP009273/3030444‑3030741
                                                                                                                                                    |                                                                                                                                                     
AGGGCTTCAATTTGCATTCCTTGTTCGATCTCTTTTCGCGTCTGGTTTAGCGCATCGAGTTCATTTGCCAGCACGCGCGCTTCGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATAG                                                                                                                                                      >  SRR19973065.932920/1‑150 (MQ=60)
AGGGCTTCAATTTGCATTCCTTGTTCGATCTCTTTTCGCGTCTGGTTTAGCGCATCGAGTTCATTTGCCAGCACGCGCGCTTCGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATAG                                                                                                                                                      >  SRR19973065.1475938/1‑150 (MQ=60)
 GGGCTTCAATTTGCATTCCTTGTTCGATCTCTTTTCGCGTCTGGTTTAGCGCATCGAGTTCATTTGCCAGCACGCGCGCTTCGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATAGA                                                                                                                                                     >  SRR19973065.1293033/1‑150 (MQ=60)
    CTTCAATTTGCATTCCTTGTTCGATCTCTTTTCGCGTCTGGTTTAGCGCATCGAGTTCATTTGCCAGCACGCGCGCTTCGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATAGAGAC                                                                                                                                                  >  SRR19973065.2528249/1‑150 (MQ=60)
    CTTCAATTTGCATTCCTTGTTCGATCTCTTTTCGCGTCTGGTTTAGCGCATCGAGTTCATTTGCCAGCACGCGCGCTTCGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATAGAGAC                                                                                                                                                  >  SRR19973065.756981/1‑150 (MQ=60)
    CTTCAATTTGCATTCCTTGTTCGATCTCTTTTCGCGTCTGGTTTAGCGCATCGAGTTCATTTGCCAGCACGCGCGCTTCGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATAGAGAC                                                                                                                                                  >  SRR19973065.756830/1‑150 (MQ=60)
     TTCAATTTGCATTCCTTGTTCGATCTCTTTTCGCGTCTGGTTTAGCGCATCGAGTTCATTTGCCAGCACGCGCGCTTCGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATAGAGACG                                                                                                                                                 <  SRR19973065.3149473/150‑1 (MQ=60)
     TTCAATTTGCATTCCTTGTTCGATCTCTTTTCGCGTCTGGTTTAGCGCATCGAGTTCATTTGCCAGCACGCGCGCTTCGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATAGAGACG                                                                                                                                                 <  SRR19973065.3392017/150‑1 (MQ=60)
     TTCAATTTGCATTCCTTGTTCGATCTCTTTTCGCGTCTGGTTTAGCGCATCGAGTTCATTTGCCAGCACGCGCGCTTCGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATAGAGACG                                                                                                                                                 <  SRR19973065.71757/150‑1 (MQ=60)
     TTCAATTTGCATTCCTTGTTCGATCTCTTTTCGCGTCTGGTTTAGCGCATCGAGTTCATTTGCCAGCACGCGCGCTTCGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATAGAGACG                                                                                                                                                 <  SRR19973065.70971/150‑1 (MQ=60)
     TTCAATTTGCATTCCTTGTTCGATCTCTTTTCGCGTCTGGTTTAGCGCATCGAGTTCATTTGCCAGCACGCGCGCTTCGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATAGAGACG                                                                                                                                                 <  SRR19973065.2565331/150‑1 (MQ=60)
      TCAATTTGCATTCCTTGTTCGATCTCTTTTCGCGTCTGGTTTAGCGCATCGAGTTCATTTGCCAGCACGCGCGCTTCGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATAGAGACGT                                                                                                                                                >  SRR19973065.1391871/1‑150 (MQ=60)
      TCAATTTGCATTCCTTGTTCGATCTCTTTTCGCGTCTGGTTTAGCGCATCGAGTTCATTTGCCAGCACGCGCGCTTCGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATAGAGACGT                                                                                                                                                >  SRR19973065.1391794/1‑150 (MQ=60)
       CAATTTGCATTCCTTGTTCGATCTCTTTTCGCGTCTGGTTTAGCGCATCGAGTTCATTTGCCAGCACGCGCGCTTCGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATAGAGACGTG                                                                                                                                               <  SRR19973065.1589790/150‑1 (MQ=60)
       CAATTTGCATTCCTTGTTCGATCTCTTTTCGCGTCTGGTTTAGCGCATCGAGTTCATTTGCCAGCACGCGCGCTTCGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATAGAGACGTG                                                                                                                                               >  SRR19973065.268985/1‑150 (MQ=60)
       CAATTTGCATTCCTTGTTCGATCTCTTTTCGCGTCTGGTTTAGCGCATCGAGTTCATTTGCCAGCACGCGCGCTTCGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATAGAGACGTG                                                                                                                                               <  SRR19973065.1589890/150‑1 (MQ=60)
        AATTTGCATTCCTTGTTCGATCTCTTTTCGCGTCTGGTTTAGCGCATCGAGTTCATTTGCCAGCACGCGCGCTTCGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATAGAGACGTGG                                                                                                                                              >  SRR19973065.2549774/1‑150 (MQ=60)
         ATTTGCATTCCTTGTTCGATCTCTTTTCGCGTCTGGTTTAGCGCATCGAGTTCATTTGCCAGCACGCGCGCTTCGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATAGAGACGTGGC                                                                                                                                             >  SRR19973065.1756004/1‑150 (MQ=60)
            TGCATTCCTTGTTCGATCTCTTTTCGCGTCTGGTTTAGCGCATCGAGTTCATTTGCCAGCACGCGCGCTTCGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATAGAGACGTGGCCCC                                                                                                                                          >  SRR19973065.393275/1‑150 (MQ=60)
            TGCATTCCTTGTTCGATCTCTTTTCGCGTCTGGTTTAGCGCATCGAGTTCATTTGCCAGCACGCGCGCTTCGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATAGAGACGTGGCCCC                                                                                                                                          <  SRR19973065.546611/150‑1 (MQ=60)
            TGCATTCCTTGTTCGATCTCTTTTCGCGTCTGGTTTAGCGCATCGAGTTCATTTGCCAGCACGCGCGCTTCGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATAGAGACGTGGCCCC                                                                                                                                          <  SRR19973065.2465016/150‑1 (MQ=60)
             GCATTCCTTGTTCGATCTCTTTTCGCGTCTGGTTTAGCGCATCGAGTTCATTTGCCAGCACGCGCGCTTCGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATAGAGACGTGGCCCCA                                                                                                                                         <  SRR19973065.2183525/150‑1 (MQ=60)
               ATTCCTTGTTCGATCTCTTTTCGCGTCTGGTTTAGCGCATCGAGTTCATTTGCCAGCACGCGCGCTTCGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATAGAGACGTGGCCCCAGC                                                                                                                                       >  SRR19973065.806189/1‑150 (MQ=60)
                  CCTTGTTCGATCTCTTTTCGCGTCTGGTTTAGCGCATCGAGTTCATTTGCCAGCACGCGCGCTTCGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATAGAGACGTGGCCCCAGCGCA                                                                                                                                    >  SRR19973065.1680956/1‑150 (MQ=60)
                  CCTTGTTCGATCTCTTTTCGCGTCTGGTTTAGCGCATCGAGTTCATTTGCCAGCACGCGCGCTTCGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATAGAGACGTGGCCCCAGCGCA                                                                                                                                    >  SRR19973065.2287224/1‑150 (MQ=60)
                  CCTTGTTCGATCTCTTTTCGCGTCTGGTTTAGCGCATCGAGTTCATTTGCCAGCACGCGCGCTTCGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATAGAGACGTGGCCCCAGCGCA                                                                                                                                    >  SRR19973065.2294004/1‑150 (MQ=60)
                     TGTTCGATCTCTTTTCGCGTCTGGTTTAGCGCATCGAGTTCATTTGCCAGCACGCGCGCTTCGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATAGAGACGTGGCCCCAGCGCAAAA                                                                                                                                 >  SRR19973065.1440934/1‑150 (MQ=60)
                      GTTCGATCTCTTTTCGCGTCTGGTTTAGCGCATCGAGTTCATTTGCCAGCACGCGCGCTTCGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATAGAGACGTGGCCCCAGCGCAAAAC                                                                                                                                >  SRR19973065.3268124/1‑150 (MQ=60)
                      GTTCGATCTCTTTTCGCGTCTGGTTTAGCGCATCGAGTTCATTTGCCAGCACGCGCGCTTCGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATAGAGACGTGGCCCCAGCGCAAAAC                                                                                                                                <  SRR19973065.320370/150‑1 (MQ=60)
                      GTTCGATCTCTTTTCGCGTCTGGTTTAGCGCATCGAGTTCATTTGCCAGCACGCGCGCTTCGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATAGAGACGTGGCCCCAGCGCAAAAC                                                                                                                                >  SRR19973065.1517096/1‑150 (MQ=60)
                         CGATCTCTTTTCGCGTCTGGTTTAGCGCATCGAGTTCATTTGCCAGCACGCGCGCTTCGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATAGAGACGTGGCCCCAGCGCAAAACCTA                                                                                                                             >  SRR19973065.3293271/1‑150 (MQ=60)
                          GATCTCTTTTCGCGTCTGGTTTAGCGCATCGAGTTCATTTGCCAGCACGCGCGCTTCGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATAGAGACGTGGCCCCAGCGCAAAACCTAA                                                                                                                            >  SRR19973065.361694/1‑150 (MQ=60)
                            TCTCTTTTCGCGTCTGGTTTAGCGCATCGAGTTCATTTGCCAGCACGCGCGCTTCGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATAGAGACGTGGCCCCAGCGCAAAACCTAAAT                                                                                                                          >  SRR19973065.2669718/1‑150 (MQ=60)
                               CTTTTCGCGTCTGGTTTAGCGCATCGAGTTCATTTGCCAGCACGCGCGCTTCGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATAGAGACGTGGCCCCAGCGCAAAACCTAAATCGC                                                                                                                       >  SRR19973065.652006/1‑150 (MQ=60)
                               CTTTTCGCGTCTGGTTTAGCGCATCGAGTTCATTTGCCAGCACGCGCGCTTCGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATAGAGACGTGGCCCCAGCGCAAAACCTAAATCGC                                                                                                                       >  SRR19973065.1105281/1‑150 (MQ=60)
                               CTTTTCGCGTCTGGTTTAGCGCATCGAGTTCATTTGCCAGCACGCGCGCTTCGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATAGAGACGTGGCCCCAGCGCAAAACCTAAATCGC                                                                                                                       >  SRR19973065.1106690/1‑150 (MQ=60)
                                 TTTCGCGTCTGGTTTAGCGCATCGAGTTCATTTGCCAGCACGCGCGCTTCGCGGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATAGAGACGTGGCCCCAGCGCAAAACCTAACTCGCTG                                                                                                                     <  SRR19973065.653609/150‑1 (MQ=60)
                                    CGCGTCTGGTTTAGCGCATCGAGTTCATTTGCCAGCACGCGCGCTTCGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATAGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCG                                                                                                                  >  SRR19973065.125487/1‑150 (MQ=60)
                                    CGCGTCTGGTTTAGCGCATCGAGTTCATTTGCCAGCACGCGCGCTTCGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATAGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCG                                                                                                                  >  SRR19973065.125495/1‑150 (MQ=60)
                                       GTCTGGTTTAGCGCATCGAGTTCATTTGCCAGCACGCGCGCTTCGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATAGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCG                                                                                                               >  SRR19973065.2646708/1‑150 (MQ=60)
                                       GTCTGGTTTAGCGCATCGAGTTCATTTGCCAGCACGCGCGCTTCGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATAGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCG                                                                                                               <  SRR19973065.2255206/150‑1 (MQ=60)
                                       GTCTGGTTTAGCGCATCGAGTTCATTTGCCAGCACGCGCGCTTCGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATAGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCG                                                                                                               >  SRR19973065.879090/1‑150 (MQ=60)
                                       GTCTGGTTTAGCGCATCGAGTTCATTTGCCAGCACGCGCGCTTCGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATAGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCG                                                                                                               <  SRR19973065.2254867/150‑1 (MQ=60)
                                       GTCTGGTTTAGCGCATCGAGTTCATTTGCCAGCACGCGCGCTTCGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATAGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCG                                                                                                               <  SRR19973065.2254735/150‑1 (MQ=60)
                                         CTGGTTTAGCGCATCGAGTTCATTTGCCAGCACGCGCGCTTCGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATAGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAG                                                                                                             <  SRR19973065.2035245/150‑1 (MQ=60)
                                           GGTTTAGCGCATCGAGTTCATTTGCCAGCACGCGCGCTTCGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATAGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTT                                                                                                           <  SRR19973065.1893802/150‑1 (MQ=60)
                                            GTTTAGCGCATCGAGTTCATTTGCCAGCACGCGCGCTTCGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATAGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTT                                                                                                          >  SRR19973065.629016/1‑150 (MQ=60)
                                              TTAGCGCATCGAGTTCATTTGCCAGCACGCGCGCTTCGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATAGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTT                                                                                                        <  SRR19973065.39604/150‑1 (MQ=60)
                                              TTAGCGCATCGAGTTCATTTGCCAGCACGCGCGCTTCGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATAGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTT                                                                                                        <  SRR19973065.151759/150‑1 (MQ=60)
                                                   GCATCGAGTTCATTTGCCAGCACGCGCGCTTCGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATAGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCA                                                                                                   <  SRR19973065.2324780/150‑1 (MQ=60)
                                                       CGAGTTCATTTGCCAGCACGCGCGCTTCGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATAGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCATCAC                                                                                               <  SRR19973065.1270225/150‑1 (MQ=60)
                                                       CGAGTTCATTTGCCAGCACGCGCGCTTCGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATAGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCATCAC                                                                                               <  SRR19973065.1270188/150‑1 (MQ=60)
                                                       CGAGTTCATTTGCCAGCACGCGCGCTTCGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATAGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCATCAC                                                                                               <  SRR19973065.1678794/150‑1 (MQ=60)
                                                        GAGTTCATTTGCCAGCACGCGCGCTTCGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATAGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCATCACG                                                                                              >  SRR19973065.297094/1‑150 (MQ=60)
                                                            TCATTTGCCAGCACGCGCGCTTCGCAGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATAGAGACGTGGCCCCATCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCATCACGGTTT                                                                                          <  SRR19973065.560298/150‑1 (MQ=60)
                                                              ATTTGCCAGCACGCGCGCTTCGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATAGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCATCACGGTTTGC                                                                                        >  SRR19973065.1912215/1‑150 (MQ=60)
                                                               TTTGCCAGCACGCGCGCTTCGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATAGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCATCACGGTTTGCC                                                                                       <  SRR19973065.899623/150‑1 (MQ=60)
                                                               TTTGCCAGCACGCGCGCTTCGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATAGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCATCACGGTTTGCC                                                                                       <  SRR19973065.2913712/150‑1 (MQ=60)
                                                                TTGCCAGCACGCGCGCTTCGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATAGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCATCACGGTTTGCCA                                                                                      <  SRR19973065.1446642/150‑1 (MQ=60)
                                                                TTGCCAGCACGCGCGCTTCGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATAGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCATCACGGTTTGCCA                                                                                      <  SRR19973065.3203148/150‑1 (MQ=60)
                                                                 TGCCAGCACGCGCGCTTCGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATAGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCATCACGGTTTGCCAC                                                                                     <  SRR19973065.168006/150‑1 (MQ=60)
                                                                  GCCAGCACGCGCGCTTCGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATAGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCATCACGGTTTGCCACT                                                                                    <  SRR19973065.171566/150‑1 (MQ=60)
                                                                   CCAGCACGCGCGCTTCGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATAGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCATCACGGTTTGCCACTT                                                                                   <  SRR19973065.1785313/150‑1 (MQ=60)
                                                                   CCAGCACGCGCGCTTCGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATAGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCATCACGGTTTGCCACTT                                                                                   <  SRR19973065.3530370/150‑1 (MQ=60)
                                                                    CAGCACGCGCGCTTCGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATAGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCATCACGGTTTGCCACTTC                                                                                  >  SRR19973065.12163/1‑150 (MQ=60)
                                                                     AGCACGCGCGCTTCGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATAGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCATCACGGTTTGCCACTTCA                                                                                 <  SRR19973065.781180/150‑1 (MQ=60)
                                                                          GCGCGCTTCGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATAGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCATCACGGTTTGCCACTTCAAGCAG                                                                            <  SRR19973065.2828832/150‑1 (MQ=60)
                                                                                TTCGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATAGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCATCACGGTTTGCCACTTCAAGCAGCGCTTT                                                                      <  SRR19973065.2980574/150‑1 (MQ=60)
                                                                                 TCGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATAGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCATCACGGTTTGCCACTTCAAGCAGCGCTTTA                                                                     <  SRR19973065.1741596/150‑1 (MQ=60)
                                                                                 TCGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATAGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCATCACGGTTTGCCACTTCAAGCAGCGCTTTA                                                                     <  SRR19973065.229285/150‑1 (MQ=60)
                                                                                   GCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATAGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCATCACGGTTTGCCACTTCAAGCAGCGCTTTAAT                                                                   <  SRR19973065.702827/150‑1 (MQ=60)
                                                                                     CGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATAGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCATCACGGTTTGCCACTTCAAGCAGCGCTTTAATCC                                                                 <  SRR19973065.1079717/150‑1 (MQ=60)
                                                                                      GATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATAGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCATCACGGTTTGCCACTTCAAGCAGCGCTTTAATCCC                                                                >  SRR19973065.27230/1‑150 (MQ=60)
                                                                                      GATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATAGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCATCACGGTTTGCCACTTCAAGCAGCGCTTTAATCCC                                                                >  SRR19973065.1241091/1‑150 (MQ=60)
                                                                                       ATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATAGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCATCACGGTTTGCCACTTCAAGCAGCGCTTTAATCCCC                                                               >  SRR19973065.3476423/1‑150 (MQ=60)
                                                                                       ATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATAGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCATCACGGTTTGCCACTTCAAGCAGCGCTTTAATCCCC                                                               <  SRR19973065.3353804/150‑1 (MQ=60)
                                                                                         GTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATAGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCATCACGGTTTGCCACTTCAAGCAGCGCTTTAATCCCCGG                                                             >  SRR19973065.1028450/1‑150 (MQ=60)
                                                                                           TGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATAGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCATCACGGTTTGCCACTTCAAGCAGCGCTTTAATCCCCGGAC                                                           >  SRR19973065.2573018/1‑150 (MQ=60)
                                                                                           TGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATAGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCATCACGGTTTGCCACTTCAAGCAGCGCTTTAATCCCCGGAC                                                           >  SRR19973065.2575129/1‑150 (MQ=60)
                                                                                                CACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATAGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCATCACGGTTTGCCACTTCAAGCAGCGCTTTAATCCCCGGACGGCAC                                                      >  SRR19973065.713052/1‑150 (MQ=60)
                                                                                                CACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATAGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCATCACGGTTTGCCACTTCAAGCAGCGCTTTAATCCCCGGACGGCAC                                                      >  SRR19973065.713190/1‑150 (MQ=60)
                                                                                                    ACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATAGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCATCACGGTTTGCCACTTCAAGCAGCGCTTTAATCCCCGGACGGCACTTTC                                                  <  SRR19973065.3339282/150‑1 (MQ=60)
                                                                                                        CAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATAGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCATCACGGTTTGCCACTTCAAGCAGCGCTTTAATCCCCGGACGGCACTTTCCGGC                                              <  SRR19973065.2298705/150‑1 (MQ=60)
                                                                                                             CCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATAGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTTCATCACGGTTTGCCACTTCAAGCAGCGCTTTAATCCCCGGACGGCACTTTCCGGCTCGGA                                         >  SRR19973065.3055143/1‑150 (MQ=60)
                                                                                                             CCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATAGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCATCACGGTTTGCCACTTCAAGCAGCGCTTTAATCCCCGGACGGCACTTTCCGGCTCGGA                                         <  SRR19973065.3191206/150‑1 (MQ=60)
                                                                                                              CACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATAGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCATCACGGTTTGCCACTTCAAGCAGCGCTTTAATCCCCGGACGGCACTTTCCGGCTCGGAT                                        >  SRR19973065.2339993/1‑150 (MQ=60)
                                                                                                                 ACCGACGGACATATCGTCCAGTCGTCCGGCAGCATAGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCATCACGGTTTGCCACTTCAAGCAGCGCTTTAATCCCCGGACGGCACTTTCCGGCTCGGATGCG                                     <  SRR19973065.1253375/150‑1 (MQ=60)
                                                                                                                    GACGGACATATCGTCCAGTCGTCCGGCAGCATAGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCATCACGGTTTGCCACTTCAAGCAGCGCTTTAATCCCCGGACGGCACTTTCCGGCTCGGATGCGACT                                  <  SRR19973065.1719479/150‑1 (MQ=60)
                                                                                                                    GACGGACATATCGTCCAGTCGTCCGGCAGCATAGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCATCACGGTTTGCCACTTCAAGCAGCGCTTTAATCCCCGGACGGCACTTTCCGGCTCGGATGCGACT                                  <  SRR19973065.2405543/150‑1 (MQ=60)
                                                                                                                    GACGGACATATCGTCCAGTCGTCCGGCAGCATAGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCATCACGGTTTGCCACTTCAAGCAGCGCTTTAATCCCCGGACGGCACTTTCCGGCTCGGATGCGACT                                  <  SRR19973065.2405501/150‑1 (MQ=60)
                                                                                                                          CATATCGTCCAGTCGTCCGGCAGCATAGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCATCACGGTTTGCCACTTCAAGCAGCGCTTTAATCCCCGGACGGCACTTTCCGGCTCGGATGCGACTCATCCC                            <  SRR19973065.3399385/150‑1 (MQ=60)
                                                                                                                           ATATCGTCCAGTCGTCCGGCAGCATAGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCATCACGGTTTGCCACTTCAAGCAGCGCTTTAATCCCCGGACGGCACTTTCCGGCTCGGATGCGACTCATCCCC                           >  SRR19973065.2150586/1‑150 (MQ=60)
                                                                                                                           ATATCGTCCAGTCGTCCGGCAGCATAGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCATCACGGTTTGCCACTTCAAGCAGCGCTTTAATCCCCGGACGGCACTTTCCGGCTCGGATGCGACTCATCCCC                           >  SRR19973065.2494826/1‑150 (MQ=60)
                                                                                                                           ATATCGTCCAGTCGTCCGGCAGCATAGAGACGTGGACCCAGCGCAAAACCTAAATCGCAGGCGGCCAGTTATTGTCAATCACGGAAAGCCACTTCAACCAGCGCTTTAAaaacagcaccccactttccgccacgaatgcgacacaaccca                           >  SRR19973065.2150555/1‑109 (MQ=56)
                                                                                                                            TATCGTCCAGTCGTCCGGCAGCATAGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCATCACGGTTTGCCACTTCAAGCAGCGCTTTAATCCCCGGACGGCACTTTCCGGCTCGGATGCGACTCATCCCCT                          <  SRR19973065.1884943/150‑1 (MQ=60)
                                                                                                                            TATCGTCCAGTCGTCCGGCAGCATAGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCATCACGGTTTGCCACTTCAAGCAGCGCTTTAATCCCCGGACGGCACTTTCCGGCTCGGATGCGACTCATCCCCT                          <  SRR19973065.3022986/150‑1 (MQ=60)
                                                                                                                                      TCGTCCGGCAGCATAGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCATCACGGTTTGCCACTTCAAGCAGCGCTTTAATCCCCGGACGGCACTTTCCGGCTCGGATGCGACTCATCCCCTGCCAGGTCAG                <  SRR19973065.3373374/150‑1 (MQ=60)
                                                                                                                                        GTCCGGCAGCATAGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCATCACGGTTTGCCACTTCAAGCAGCGCTTTAATCCCCGGACGGCACTTTCCGGCTCGGATGCGACTCATCCCCTGCCAGGTCAGAA              >  SRR19973065.3065381/1‑150 (MQ=60)
                                                                                                                                         TCCGGCAGCATAGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCATCACGGTTTGCCACTTCAAGCAGCGCTTTAATCCCCGGACGGCACTTTCCGGCTCGGATGCGACTCATCCCCTGCCAGGTCAGAAT             <  SRR19973065.1119049/150‑1 (MQ=60)
                                                                                                                                         TCCGGCAGCATAGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCATCACGGTTTGCCACTTCAAGCAGCGCTTTAATCCCCGGACGGCACTTTCCGGCTCGGATGCGACTCATCCCCTGCCAGGTCAGAAT             <  SRR19973065.1625342/150‑1 (MQ=60)
                                                                                                                                           CGGCAGCATAGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCATCACGGTTTGCCACTTCAAGCAGCGCTTTAATCCCCGGACGGCACTTTCCGGCTCGGATGCGACTCATCCCCTGCCAGGTCAGAATGC           <  SRR19973065.543384/150‑1 (MQ=60)
                                                                                                                                           CGGCAGCATAGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCATCACGGTTTGCCACTTCAAGCAGCGCTTTAATCCCCGGACGGCACTTTCCGGCTCGGATGCGACTCATCCCCTGCCAGGTCAGAATGC           >  SRR19973065.2021815/1‑150 (MQ=60)
                                                                                                                                           CGGCAGCATAGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCATCACGGTTTGCCACTTCAAGCAGCGCTTTAATCCCCGGACGGCACTTTCCGGCTCGGATGCGACTCATCCCCTGCCAGGTCAGAATGC           <  SRR19973065.543954/150‑1 (MQ=60)
                                                                                                                                           CGGCAGCATAGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCATCACGGTTTGCCACTTCAAGCAGCGCTTTAATCCCCGGACGGCACTTTCCGGCTCGGATGCGACTCATCCCCTGCCAGGTCAGAATGC           <  SRR19973065.543369/150‑1 (MQ=60)
                                                                                                                                              CAGCATAGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCATCACGGTTTGCCACTTCAAGCAGCGCTTTAATCCCCGGACGGCACTTTCCGGCTCGGATGCGACTCATCCCCTGCCAGGTCAGAATGCGAT        <  SRR19973065.3312558/150‑1 (MQ=60)
                                                                                                                                              CAGCATAGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCATCACGGTTTGCCACTTCAAGCAGCGCTTTAATCCCCGGACGGCACTTTCCGGCTCGGATGCGACTCATCCCCTGCCAGGTCAGAATGCGAT        <  SRR19973065.3478977/150‑1 (MQ=60)
                                                                                                                                              CAGCATAGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCATCACGGTTTGCCACTTCAAGCAGCGCTTTAATCCCCGGACGGCACTTTCCGGCTCGGATGCGACTCATCACCTGCCAGGTCAGAATGCGAT        <  SRR19973065.1454318/150‑1 (MQ=60)
                                                                                                                                                GCATAGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCATCACGGTTTGCCACTTCAAGCAGCGCTTTAATCCCCGGACGGCACTTTCCGGCTCGGATGCGACTCATCCCCTGCCAGGTCAGAATGCGATTA      <  SRR19973065.3473782/150‑1 (MQ=60)
                                                                                                                                                 CATAGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCATCACGGTTTGCCACTTCAAGCAGCGCTTTAATCCCCGGACGGCACTTTCCGGCTCGGATGCGACTCATCCCCTGCCAGGTCAGAATGCGATTAT     <  SRR19973065.1376812/150‑1 (MQ=60)
                                                                                                                                                 CATAGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCCAGTTTTTGTGCATCACGGTTTGCCACTTCAAGCAGCGCTTTAATCCCCGGACGGCACGTTCCGGCTCGGATGCGACTCATCACCTGACAGTTCAGAATGCGATTAT     >  SRR19973065.941610/1‑150 (MQ=60)
                                                                                                                                                  ATAGAGACGTGGCCCCAGCGCAAAACCTAAGTCGCTGGCGGCGAGTTTTTGTGCATCACGGTTTGCCACTTCAAGCAGCGCTTTAATCCCCGGACGGCACTTTCCGGCTCGGATGCGACTCATCCCCTGCCAGGTCAGAATGCGATTATT    <  SRR19973065.2530611/150‑1 (MQ=60)
                                                                                                                                                  ATAGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCATCACGGTTTGCCACTTCAAGCAGCGCTTTAATCCCCGGACGGCACTTTCCGGCTCGGATGCGACTCATCCCCTGCCAGGTCAGAATGCGATTATT    >  SRR19973065.2721382/1‑150 (MQ=60)
                                                                                                                                                    AGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCATCACGGTTTGCCACTTCAAGCAGCGCTTTAATCCCCGGACGGCACTTTCCGGCTCGGATGCGACTCATCCCCTGCCAGGTCAGAATGCGATTATTAG  <  SRR19973065.1676732/150‑1 (MQ=60)
                                                                                                                                                    |                                                                                                                                                     
AGGGCTTCAATTTGCATTCCTTGTTCGATCTCTTTTCGCGTCTGGTTTAGCGCATCGAGTTCATTTGCCAGCACGCGCGCTTCGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATTGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCATCACGGTTTGCCACTTCAAGCAGCGCTTTAATCCCCGGACGGCACTTTCCGGCTCGGATGCGACTCATCCCCTGCCAGGTCAGAATGCGATTATTAG  >  CP009273/3030444‑3030741

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑