Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F434 I0 R1 1130 215.9 7031662 97.1% 6827743 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,058,809 G→T 100% I106I (ATC→ATA)  yihO ← putative transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,058,8090GT95.7% 618.7 / 10.4 224I106I (ATC→ATA) yihOputative transporter
Reads supporting (aligned to +/- strand):  ref base G (11/1);  new base T (97/114);  total (109/115)
Fisher's exact test for biased strand distribution p-value = 2.08e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 224 total aligned reads displayed.

AGCTGCGCGCGCTCATTCGGGTTTTTGGTAATTGCCGGGATCATCGCACCATACGAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCGATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATATTTTTCCGCGAGTCGAGTAAAAATCCGGTGAGCATATCGGTAAACGCGGTAAAAAACTTCGC  >  CP009273/4058666‑4058952
                                                                                                                                               |                                                                                                                                               
aGCTGCGCGCGCTCATTCGGGTTTTTGGTAATTGCCGGGATCATCGCACCATACGAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCGATaa                                                                                                                                             >  1:979044/1‑148 (MQ=255)
aGCTGCGCGCGCTCATTCGGGTTTTTGGTAATTGCCGGGATCATCGCACCATACGAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCGATaa                                                                                                                                             >  1:3401686/1‑148 (MQ=255)
   tGCGCGCGCTCATTCGGGTTTTTGGTAATTGCCGGGATCATCGCACCATACGAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACt                                                                                                                                          <  1:1375428/148‑1 (MQ=255)
      gcgcgcTCATTCGGGTTTTTGGTAATTGCCGGGATCATCGCACCATACGAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGa                                                                                                                                       <  2:1868480/148‑1 (MQ=255)
       cgcgcTCATTCGGGTTTTTGGTAATTGCCGGGATCATCGCACCATACGAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAg                                                                                                                                      <  1:791352/148‑1 (MQ=255)
             cATTCGGGTTTTTGGTAATTGCCGGGATCATCGCACCATACGAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGc                                                                                                                                >  1:889138/1‑148 (MQ=255)
               ttCGGGTTTTTGGTAATTGCCGGGATCATCGCACCATACGAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCaa                                                                                                                              <  1:1753831/148‑1 (MQ=255)
               ttCGGGTTTTTGGTAATTGCCGGGATCATCGCACCATACGAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCaa                                                                                                                              <  1:3490199/148‑1 (MQ=255)
               ttCGGGTTTTTGGTAATTGCCGGGATCATCGCACCATACGAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCaa                                                                                                                              >  2:391809/1‑148 (MQ=255)
                tCGGGTTTTTGGTAATTGCCGGGATCATCGCACCATACGAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAAt                                                                                                                             <  2:1797299/148‑1 (MQ=255)
                  gggTTTTTGGTAATTGCCGGGATCATCGCACCATACGAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTa                                                                                                                           >  2:3176529/1‑148 (MQ=255)
                   ggTTTTTGGTAATTGCCGGGATCATCGCACCATACGAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGGTGCAATTaa                                                                                                                          >  2:2774402/1‑148 (MQ=255)
                     tttttGGTAATTGCCGGGATCATCGCACCATACGAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAAcg                                                                                                                        <  1:106517/148‑1 (MQ=255)
                     tttttGGTAATTGCCGGGATCATCGCACCATACGAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAAcg                                                                                                                        <  1:1794844/148‑1 (MQ=255)
                     tttttGGTAATTGCCGGGATCATCGCACCATACGAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAAcg                                                                                                                        <  1:1028308/148‑1 (MQ=255)
                      ttttGGTAATTGCCGGGATCATCGCACCATACGAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAAcgc                                                                                                                       >  1:113694/1‑148 (MQ=255)
                        ttGGTAATTGCCGGGATCATCGCACCATACGAGCAGTTCATCAGACTGTATTAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAAcgccg                                                                                                                     <  1:2722670/148‑1 (MQ=255)
                         tGGTAATTGCCGGGATCATCGCACCATACGAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAAcgccgc                                                                                                                    <  2:3027218/148‑1 (MQ=255)
                           gTAATTGCCGGGATCATCGCACCATACGAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGccgccg                                                                                                                  <  2:729286/148‑1 (MQ=255)
                            tAATTGCCGGGATCATCGCACCATACGAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCgg                                                                                                                 >  2:376644/1‑148 (MQ=255)
                             aaTTGCCGGGATCATCGCACCATACGAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGa                                                                                                                <  2:3151974/148‑1 (MQ=255)
                              aTTGCCGGGATCATCGCACCATACGAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGaa                                                                                                               <  2:3218447/148‑1 (MQ=255)
                                 gCCGGGATCATCGCACCATACGAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCg                                                                                                            <  1:2788655/148‑1 (MQ=255)
                                    gggATCATCGCACCATACGAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTg                                                                                                         <  2:1557267/148‑1 (MQ=255)
                                    gggATCATCGCACCATACGAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCGATAAACTGGAGCGGTGCAATTAACGCCGCCGGAACCGCTg                                                                                                         >  1:2341440/1‑148 (MQ=255)
                                     ggATCATCGCACCATACGAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGc                                                                                                        >  2:3498853/1‑148 (MQ=255)
                                     ggATCATCGCACCATACGAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGc                                                                                                        >  1:352488/1‑148 (MQ=255)
                                     ggATCATCGCACCATACGAGCAGTACATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGTCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGc                                                                                                        <  2:380711/148‑1 (MQ=255)
                                      gATCATCGCACCATACGAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCa                                                                                                       <  2:2083921/148‑1 (MQ=255)
                                       atcatcGCACCATACGAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCCAACCAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCat                                                                                                      >  1:1230134/1‑148 (MQ=255)
                                       atcatcGCACCATACGAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCat                                                                                                      <  2:1250286/148‑1 (MQ=255)
                                       atcatcGCACCATACGAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCat                                                                                                      <  2:2284579/148‑1 (MQ=255)
                                         catcGCACCATACGAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCGATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCatat                                                                                                    >  1:257406/1‑148 (MQ=255)
                                            cGCACCATACGAGCAGTTCATCAGACTGTATGAAAGTCCAACCATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATataaa                                                                                                 <  2:2079894/148‑1 (MQ=255)
                                              cACCATACGAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATataaaat                                                                                               <  1:1485068/148‑1 (MQ=255)
                                                ccATACGAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATataaaataa                                                                                             >  1:419334/1‑148 (MQ=255)
                                                ccATACGAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCGATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATataaaataa                                                                                             <  1:1933605/148‑1 (MQ=255)
                                                   tccGAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAgg                                                                                          <  2:1001352/146‑1 (MQ=255)
                                                   tACGAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAATGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATataaaataaatg                                                                                          >  1:712715/1‑146 (MQ=255)
                                                       aGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCCAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACg                                                                                      >  1:76890/1‑148 (MQ=255)
                                                       aGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACg                                                                                      >  2:1755823/1‑148 (MQ=255)
                                                        gCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGa                                                                                     >  2:1665614/1‑148 (MQ=255)
                                                         cAGTTCATCATACTGTATGAAAGTCAAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGaa                                                                                    <  1:2671910/148‑1 (MQ=255)
                                                         cAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGaa                                                                                    <  1:2872852/148‑1 (MQ=255)
                                                          aGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAATCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGaaa                                                                                   <  2:590951/148‑1 (MQ=255)
                                                          aGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCCAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAACTAAAGGGACGaaa                                                                                   >  2:3248535/1‑148 (MQ=255)
                                                          aGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGaaa                                                                                   <  1:153204/148‑1 (MQ=255)
                                                            ttCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAAtt                                                                                 >  2:428309/1‑148 (MQ=255)
                                                            ttCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAAtt                                                                                 >  2:1969005/1‑148 (MQ=255)
                                                            ttCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAAtt                                                                                 >  2:2248396/1‑148 (MQ=255)
                                                              catcaGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTg                                                                               <  1:1254380/148‑1 (MQ=255)
                                                              catcaGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGAAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTg                                                                               <  1:1206855/148‑1 (MQ=255)
                                                               atcaGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGc                                                                              <  1:3221976/148‑1 (MQ=255)
                                                                tcaGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGcc                                                                             >  2:962895/1‑148 (MQ=255)
                                                                 caGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCt                                                                            >  1:2477711/1‑148 (MQ=255)
                                                                   gACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCttt                                                                          >  2:836391/1‑148 (MQ=255)
                                                                   gACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCttt                                                                          >  2:750981/1‑148 (MQ=255)
                                                                    aCTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTa                                                                         >  2:651341/1‑148 (MQ=255)
                                                                    aCTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTa                                                                         <  1:2421648/148‑1 (MQ=255)
                                                                       gTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTTGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCAAATAAAATAAAGGGACGAAATTATCCATTAGGc                                                                      >  2:1184844/1‑148 (MQ=255)
                                                                       gTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGc                                                                      >  1:2328139/1‑148 (MQ=255)
                                                                           gAAAGTCCAAACATCATAAACAGCGCGGTGGCACTCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCaa                                                                  >  2:2208695/1‑148 (MQ=255)
                                                                            aaaGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAat                                                                 >  2:1660211/1‑148 (MQ=255)
                                                                            aaaGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAat                                                                 <  2:556576/148‑1 (MQ=255)
                                                                              aGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAATGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAatat                                                               >  1:1252855/1‑148 (MQ=255)
                                                                              aGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAatat                                                               <  2:501094/148‑1 (MQ=255)
                                                                               gTCCAAACATCATACACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATGAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATAtt                                                              <  2:2785756/148‑1 (MQ=255)
                                                                               gTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATAtt                                                              >  1:1256889/1‑148 (MQ=255)
                                                                               gTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTAGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATAtt                                                              >  2:1297844/1‑148 (MQ=255)
                                                                                 ccAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATAtttt                                                            <  1:1644642/148‑1 (MQ=255)
                                                                                 ccAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATAtttt                                                            <  2:172670/148‑1 (MQ=255)
                                                                                 ccAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGCAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATAtttt                                                            <  2:2922908/148‑1 (MQ=255)
                                                                                  cAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATAttttt                                                           <  1:1479726/148‑1 (MQ=255)
                                                                                  cAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATAttttt                                                           <  1:1479708/148‑1 (MQ=255)
                                                                                    aaCATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATATTTTTcc                                                         >  2:3420854/1‑148 (MQ=255)
                                                                                         catAAACAGCGCGGTGGCAATCGTCGTGTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATATTTTTCCGcgag                                                    >  1:465885/1‑148 (MQ=255)
                                                                                          atAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATATTTTTCCGcgagt                                                   <  1:3291356/148‑1 (MQ=255)
                                                                                           tAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATATTTTTCCGcgagtc                                                  >  2:2298590/1‑148 (MQ=255)
                                                                                            aaaCAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATATTTTTCCGcgagtcg                                                 >  2:2969523/1‑148 (MQ=255)
                                                                                               cAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATATTTTTCCGcgagtcgagt                                              >  2:3291228/1‑148 (MQ=255)
                                                                                                 gcgcGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAAAAAAGGGACGAAATTTGCCTTTAGGCCCAATATTTTTCCGCGAGTCGAGTaa                                            >  2:2614153/1‑148 (MQ=255)
                                                                                                  cgcgGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATATTTTTCCGCGAGTCGAGTaaa                                           >  2:1437072/1‑148 (MQ=255)
                                                                                                  cgcgGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATATTTTTCCGCGAGTCGAGTaaa                                           >  2:183451/1‑148 (MQ=255)
                                                                                                  cgcgGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCGATAAACTGGATCGGTGCAAATAAAGCCGCCGGAACCGCTGCCTATAAAATAAAGGGACGAAATTTTCCGTTAGGCCCAATATTTTTACGCGAGTCGAGTaaa                                           >  1:3314987/1‑148 (MQ=255)
                                                                                                     ggTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATATTTTTCCGCGAGTCGAGTAAAAAt                                        <  2:3394418/148‑1 (MQ=255)
                                                                                                     ggTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATATTTTTCCGCGAGTCGAGTAAAAAt                                        <  1:539284/148‑1 (MQ=255)
                                                                                                         gCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATATTTTTCCGCGAGTCGAGTAAAAATCCgg                                    <  2:3043827/148‑1 (MQ=255)
                                                                                                         gCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATATTTTTCCGCGAGTCGAGTAAAAATCCgg                                    <  2:3005991/148‑1 (MQ=255)
                                                                                                                 cgtTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATATTTTTCCGCGAGTCGAGTAAAAATCCGGTGAGCata                            >  1:2420229/1‑148 (MQ=255)
                                                                                                                 cgtTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATATTTTTCCGCGAGTCGAGTAAAAATCCGGTGAGCata                            >  1:2470436/1‑148 (MQ=255)
                                                                                                                  gtTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATATTTTTCCGCGAGTCGAGTAAAAATCCGGTGAGCatat                           >  1:2077070/1‑148 (MQ=255)
                                                                                                                  gtTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATATTTTTCCGCGAGTCGAGTAAAAATCCGGTGAGCatat                           <  1:2961065/148‑1 (MQ=255)
                                                                                                                             caaacaaaAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATATTTTTCCGCGAGTCGAGTAAAAATCCGGTGAGCATATCGGTAAAcgcg                <  2:2477711/148‑1 (MQ=255)
                                                                                                                              aaacaaaAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATATTTTTCCGCGAGTCGAGTAAAAATCCGGTGAGCATATCGGTAAACGCgg               <  1:202013/148‑1 (MQ=255)
                                                                                                                                 caaaAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATATTTTTCCGCGAGTCGAGTCAAAATCCGGTGAGCATATCGGTAAACGCGGTaa            >  1:2083187/1‑148 (MQ=255)
                                                                                                                                   aaaGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATATTTTTCCGCGAGTCGAGTAAAAATCCGGTGAGCATATCGGTAAACGCGGTaaaa          <  2:2328181/148‑1 (MQ=255)
                                                                                                                                    aaGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATATTTTTCCGCGAGTCGAGTAAAAATCCGGTGAGCATATCGGTAAACGCGGTaaaaa         <  1:1677094/148‑1 (MQ=255)
                                                                                                                                       gtggtgGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATATTTTTCCGCGAGTCGAGTAAAAATCCGGTGAGCATATCGGTAAACGCGGTAAAAAACt      >  2:1697037/1‑148 (MQ=255)
                                                                                                                                           tggCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATATTTTTCCGCGAGTCGAGTAAAAATCCGGTGAGCATATCGGTAAACGCGGTAAAAAACTTCGc  <  1:2247220/148‑1 (MQ=255)
                                                                                                                                           tggCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATATTTTTCCGCGAGTCGAGTAAAAATCCGGTGAGCATATCGGTAAACGCGGTAAAAAACTTCGc  <  2:113694/148‑1 (MQ=255)
                                                                                                                                               |                                                                                                                                               
AGCTGCGCGCGCTCATTCGGGTTTTTGGTAATTGCCGGGATCATCGCACCATACGAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCGATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATATTTTTCCGCGAGTCGAGTAAAAATCCGGTGAGCATATCGGTAAACGCGGTAAAAAACTTCGC  >  CP009273/4058666‑4058952

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CCGCGAGCTGCGCGCGCTCATTCGGGTTTTTGGTAATTGCCGGGATCATCGCACCATACGAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCGATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATATTTTTCCGCGAGTCGAGTAAAAATCCGGTGAGCATATCGGTAAACGCGGTAAAAAACTTCGCCA  >  CP009273/4058661‑4058954
                                                                                                                                                    |                                                                                                                                                 
CCGCGAGCTGCGCGCGCTCATTCGGGTTTTTGGTAATTGCCGGGATCATCGCACCATACGAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTTACCGGCAAAAAAAAGGTGGTGGCTA                                                                                                                                                  >  SRR19973065.3268652/1‑150 (MQ=60)
CCGCGAGCTGCGCGCGCTCATTCGGGTTTTTGGTAATTGCCGGGATCATCGCACCATACGAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTA                                                                                                                                                  <  SRR19973065.587680/150‑1 (MQ=60)
CCGCGAGCTGCGCGCGCTCATTCGGGTTTTTGGTAATTGCCGGGATCATCGCACCATACGAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTA                                                                                                                                                  >  SRR19973065.3268611/1‑150 (MQ=60)
   CGAGCTGCGCGCGCTCATTCGGGTTTTTGGTAATTGCCGGGATCATCGCACCATACGAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCGATAA                                                                                                                                               >  SRR19973065.985540/1‑150 (MQ=60)
   CGAGCTGCGCGCGCTCATTCGGGTTTTTGGTAATTGCCGGGATCATCGCACCATACGAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCGATAA                                                                                                                                               >  SRR19973065.985466/1‑150 (MQ=60)
   CGAGCTGCGCGCGCTCATTCGGGTTTTTGGTAATTGCCGGGATCATCGCACCATACGAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCGATAA                                                                                                                                               >  SRR19973065.3422478/1‑150 (MQ=60)
    GAGCTGCGCGCGCTCATTCGGGTTTTTGGTAATTGCCGGGATCATCGCACCATACGAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCCAACAAAAGTTTGTGGCTATAAA                                                                                                                                              >  SRR19973065.504450/1‑150 (MQ=60)
        TGCGCGCGCTCATTCGGGTTTTTGGTAATTGCCGGGATCATCGCACCATACGAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGG                                                                                                                                          <  SRR19973065.1384503/150‑1 (MQ=60)
         GCGCGCGCTCATTCGGGTTTTTGGTAATTGCCGGGATCATCGCACCATACGAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGA                                                                                                                                         <  SRR19973065.1264783/150‑1 (MQ=60)
            CGCGCTCATTCGGGTTTTTGGTAATTGCCGGGATCATCGCACCATACGAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCG                                                                                                                                      <  SRR19973065.796594/150‑1 (MQ=60)
              CGCTCATTCGGGTTTTTGGTAATTGCCGGGATCATCGCACCATACGAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCCAACAAAAGGTGGTGGCTATAAACTGGAGCGTT                                                                                                                                    >  SRR19973065.2499825/1‑150 (MQ=60)
               GCTCATTCGGGTTTTTGGTAATTGCCGGGATCATCGCACCATACGAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCCAACAAAAGGTGGTGGCTATAAAATGGAGCGTTT                                                                                                                                   >  SRR19973065.3423965/1‑150 (MQ=60)
                CTCATTCGGGTTTTTGGTAATTGCCGGGATCATCGCACCATACGAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGC                                                                                                                                  >  SRR19973065.894784/1‑150 (MQ=60)
                    TTCGGGTTTTTGGTAATTGCCGGGATCATCGCACCATACGAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATT                                                                                                                              <  SRR19973065.3511423/150‑1 (MQ=60)
                    TTCGGGTTTTTGGTAATTGCCGGGATCATCGCACCATACGAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATT                                                                                                                              <  SRR19973065.762530/150‑1 (MQ=60)
                    TTCGGGTTTTTGGTAATTGCCGGGATCATCGCACCATACGAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATT                                                                                                                              <  SRR19973065.1765370/150‑1 (MQ=60)
                        GGTTTTTGGTAATTGCCGGGATCATCGCACCATACGAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACG                                                                                                                          >  SRR19973065.2759426/1‑150 (MQ=60)
                         GTTTTTGGTAATTGCCGGGATCATCGCACCATACGAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAAAGC                                                                                                                         >  SRR19973065.114704/1‑150 (MQ=60)
                          TTTTTGGTAATTGCCGGGATCATCGCACCATACGAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCC                                                                                                                        <  SRR19973065.1035182/150‑1 (MQ=60)
                          TTTTTGGTAATTGCCGGGATCATCGCACCATACGAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCC                                                                                                                        <  SRR19973065.1806623/150‑1 (MQ=60)
                          TTTTTGGTAATTGCCGGGATCATCGCACCATACGAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCC                                                                                                                        <  SRR19973065.1035114/150‑1 (MQ=60)
                          TTTTTGGTAATTGCCGGGATCATCGCACCATACGAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCC                                                                                                                        <  SRR19973065.107488/150‑1 (MQ=60)
                             TTGGTAATTGCCGGGATCATCGCACCATACGAGCAGTTCATCAGACTGTATTAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCC                                                                                                                     <  SRR19973065.2739606/150‑1 (MQ=60)
                                 TAATTGCCGGGATCATCGCACCATACGAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTGTTAACCGGCACACAAAAGGTGGTGGCTATAAACTGGCGCGTTGCCATTAACGCCGCCGGAA                                                                                                                 >  SRR19973065.331618/1‑150 (MQ=60)
                                    TTGCCGGGATCATCGCACCATACGAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCG                                                                                                              <  SRR19973065.1564519/150‑1 (MQ=60)
                                      GCCGGGATCATCGCACCATACGAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCT                                                                                                            <  SRR19973065.2805942/150‑1 (MQ=60)
                                       CCGGGATCATCGCACCATACGAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCGATAAACTGGAGCGGTGCAATTAACGCCGCCGGAACCGCTG                                                                                                           >  SRR19973065.2356055/1‑150 (MQ=60)
                                        CGGGATCATCGCACCATACGAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGC                                                                                                          >  SRR19973065.354958/1‑150 (MQ=60)
                                         GGTATCATCGCACCATACGAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGAAATTAACGCCGCCGGAACCGCTGCA                                                                                                         <  SRR19973065.1511463/150‑1 (MQ=60)
                                          GGATCATCGCACCATACGAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCCAACCAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCAT                                                                                                        >  SRR19973065.1238183/1‑150 (MQ=60)
                                          GGATCATCGCACCATACGAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAAACGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCAT                                                                                                        >  SRR19973065.1238207/1‑150 (MQ=60)
                                            ATCATCGCACCATACGAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCGATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATAT                                                                                                      >  SRR19973065.259194/1‑150 (MQ=60)
                                            ATCATCGCACCATACGAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTGTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCCATTAACGCCGCCGGAACCGCGGCATAT                                                                                                      >  SRR19973065.2375580/1‑150 (MQ=60)
                                                   CACCATACGAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAA                                                                                               >  SRR19973065.422263/1‑150 (MQ=60)
                                                   CACCATACGAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAA                                                                                               <  SRR19973065.1494541/150‑1 (MQ=60)
                                                     CCATACGAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCGATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAG                                                                                             <  SRR19973065.1946261/150‑1 (MQ=60)
                                                      CATACGAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAATGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAATG                                                                                            >  SRR19973065.717484/1‑150 (MQ=60)
                                                      CATACGAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGG                                                                                            >  SRR19973065.2902747/1‑150 (MQ=60)
                                                      CATACGAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAAACGGCAAACAAAAGGTGGTGGCGATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGG                                                                                            >  SRR19973065.2488938/1‑150 (MQ=60)
                                                       ATACGAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGG                                                                                           <  SRR19973065.1113231/150‑1 (MQ=60)
                                                       ATACGAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGG                                                                                           <  SRR19973065.37773/150‑1 (MQ=60)
                                                        TACGAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGA                                                                                          >  SRR19973065.3524656/1‑150 (MQ=60)
                                                          CGAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCCAACAAAAGGTGGTGGCTATAAACTGGAGCGGTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACG                                                                                        >  SRR19973065.77672/1‑150 (MQ=60)
                                                              CAGTTCATCATACTGTATGAAAGTCAAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAAT                                                                                    <  SRR19973065.2688466/150‑1 (MQ=60)
                                                              CAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAAT                                                                                    <  SRR19973065.2890455/150‑1 (MQ=60)
                                                              CAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAAT                                                                                    <  SRR19973065.2719943/150‑1 (MQ=60)
                                                              CAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAAT                                                                                    <  SRR19973065.2719915/150‑1 (MQ=60)
                                                               AGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATT                                                                                   <  SRR19973065.154488/150‑1 (MQ=60)
                                                                   CATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCTTTTTAACCGGAAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCC                                                                               <  SRR19973065.1214620/150‑1 (MQ=60)
                                                                   CATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCC                                                                               <  SRR19973065.1262640/150‑1 (MQ=60)
                                                                   CATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCC                                                                               <  SRR19973065.2465877/150‑1 (MQ=60)
                                                                    ATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAGGGTGGTGGCGATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCT                                                                              >  SRR19973065.1445353/1‑150 (MQ=60)
                                                                    ATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCT                                                                              <  SRR19973065.3241335/150‑1 (MQ=60)
                                                                    ATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCT                                                                              >  SRR19973065.1419693/1‑150 (MQ=60)
                                                                    ATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAAATGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAATGGACGAAATTTGCCT                                                                              >  SRR19973065.2493312/1‑150 (MQ=60)
                                                                     TCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTT                                                                             <  SRR19973065.2003320/150‑1 (MQ=60)
                                                                      CAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTT                                                                            <  SRR19973065.122809/150‑1 (MQ=60)
                                                                      CAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTT                                                                            <  SRR19973065.2640772/150‑1 (MQ=60)
                                                                         ACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGG                                                                         <  SRR19973065.2436924/150‑1 (MQ=60)
                                                                         ACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGG                                                                         <  SRR19973065.2436922/150‑1 (MQ=60)
                                                                         ACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGG                                                                         <  SRR19973065.3244259/150‑1 (MQ=60)
                                                                         ACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGG                                                                         <  SRR19973065.2436923/150‑1 (MQ=60)
                                                                          CTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGC                                                                        >  SRR19973065.2342745/1‑150 (MQ=60)
                                                                          CTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGC                                                                        >  SRR19973065.2342703/1‑150 (MQ=60)
                                                                           TGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCC                                                                       <  SRR19973065.1811482/150‑1 (MQ=60)
                                                                            GTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCAGATAAAATAAAGGGACGAAATTTGCCTTTAGGCCC                                                                      >  SRR19973065.1687836/1‑150 (MQ=60)
                                                                                 AAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAATGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATAT                                                                 >  SRR19973065.1261104/1‑150 (MQ=60)
                                                                                 AAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTCTAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATAAAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATAT                                                                 >  SRR19973065.840162/1‑150 (MQ=60)
                                                                                 AAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAAAAT                                                                 >  SRR19973065.840147/1‑150 (MQ=60)
                                                                                 AAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAAATGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATAT                                                                 >  SRR19973065.1261207/1‑150 (MQ=60)
                                                                                 AAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCCGCAAACCAAAGGTGGTGGCGATAAACTGGGGCGGTGCAATTAAAGCCGCCGGAACCGCAGCATATAAAAAAAAGGGACGAAATTTTCCTGTAGGCCCAATAT                                                                 >  SRR19973065.1273018/1‑150 (MQ=60)
                                                                                 AAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAAACGGCAAACAAAATGTGGTGGCTATAAAATGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAATGGACGAAATTTTCCTTTATGCCCAATAT                                                                 >  SRR19973065.348507/1‑150 (MQ=60)
                                                                                  AAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATATT                                                                >  SRR19973065.1265163/1‑150 (MQ=60)
                                                                                  AAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATATT                                                                >  SRR19973065.3040029/1‑150 (MQ=60)
                                                                                  AAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAAACGGCAAACAAAATGTGGTGGCTATAAAATGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCCTATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATATT                                                                >  SRR19973065.3040074/1‑150 (MQ=60)
                                                                                  AAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTGTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGTATCGGTGCAATTAAAGCCGCCGGAACCGCGGCAAATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATATT                                                                >  SRR19973065.1776381/1‑150 (MQ=60)
                                                                                    GTCCAAACATCATACACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATATTTT                                                              <  SRR19973065.21451/150‑1 (MQ=60)
                                                                                      CCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATATTTTTC                                                            <  SRR19973065.1655677/150‑1 (MQ=60)
                                                                                      CCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATATTTTTC                                                            <  SRR19973065.1655682/150‑1 (MQ=60)
                                                                                       CAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATATTTTTCC                                                           <  SRR19973065.1489179/150‑1 (MQ=60)
                                                                                       CAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATATTTTTCC                                                           <  SRR19973065.1489183/150‑1 (MQ=60)
                                                                                       CAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATATTTTTCC                                                           <  SRR19973065.1489188/150‑1 (MQ=60)
                                                                                       CAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATATTTTTCC                                                           <  SRR19973065.1489165/150‑1 (MQ=60)
                                                                                            ATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATATTTTTCCGCGAG                                                      <  SRR19973065.2176885/150‑1 (MQ=60)
                                                                                            ATCATAAACAGCGCGGTGGCAATCGTCGTGTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTTCCTTTAGGCCCAATATTTTTCCGCGAG                                                      >  SRR19973065.469092/1‑150 (MQ=60)
                                                                                             TCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATATTTTTCCGCGAGT                                                     >  SRR19973065.2041832/1‑150 (MQ=60)
                                                                                             TCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATATTTTTCCGCGAGT                                                     <  SRR19973065.632450/150‑1 (MQ=60)
                                                                                               ATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATATTTTTCCGCGAGTCG                                                   <  SRR19973065.3311389/150‑1 (MQ=60)
                                                                                               ATAAACAGCGCGGTGGCAATCGTCGTGTTTACCCGCAAACAAAAGGTGGTGGCGATAAACTGGAGCCTTGCAATTAACGCCGCCGGAACCGCGGCAAATAAAAAAAAGGGACGAAATTTGCCATTATGCCCAATATTTTTAAGCGAGTCG                                                   >  SRR19973065.342177/1‑150 (MQ=60)
                                                                                                     AGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCGATAAACTGGATCGGTGCAAATAAAGCCGCCGGAACCGCTGCCTATAAAATAAAGGGACGAAATTTTCCGTTAGGCCCAATATTTTTACGCGAGTCGAGTAAA                                             >  SRR19973065.3335200/1‑150 (MQ=60)
                                                                                                          GGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATATTTTTCCGCGAGTCGAGTAAAAATCC                                        <  SRR19973065.542788/150‑1 (MQ=60)
                                                                                                            TGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATATTTTTCCGCGAGTCGAGTAAAAATCCGG                                      <  SRR19973065.542634/150‑1 (MQ=60)
                                                                                                               CAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATATTTTTCCGCGAGTCGAGTAAAAATCCGGTGA                                   <  SRR19973065.431283/150‑1 (MQ=60)
                                                                                                                    GTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATATTTTTCCGCGAGTCGAGTAAAAATCCGGTGAGCATA                              >  SRR19973065.2485994/1‑150 (MQ=60)
                                                                                                                    GTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATATTTTTCCGCGAGTCGAGTAAAAATCCGGTGAGCATA                              >  SRR19973065.2435490/1‑150 (MQ=60)
                                                                                                                     TCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATATTTTTCCGCGAGTCGAGTAAAAATCCGGTGAGCATAT                             >  SRR19973065.2091602/1‑150 (MQ=60)
                                                                                                                     TCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATATTTTTCCGCGAGTCGAGTAAAAATCCGGTGAGCATAT                             >  SRR19973065.2090349/1‑150 (MQ=60)
                                                                                                                      CGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATATTTTTCCGCGAGTCGAGTAAAAATCCGGTGAGCATATC                            <  SRR19973065.777145/150‑1 (MQ=60)
                                                                                                                       GTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATATTTTTCCGCGAGTCGAGTAAAAATCCGGTGAGCATATCG                           <  SRR19973065.2979146/150‑1 (MQ=60)
                                                                                                                       GTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATATTTTTCCGCGAGTCGAGTAAAAATCCGGTGAGCATATCG                           >  SRR19973065.2817716/1‑150 (MQ=60)
                                                                                                                                   AAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATATTTTTCCGCGAGTCGAGTAAAAATCCGGTGAGCATATCGGTAAACGCGGTA               <  SRR19973065.203570/150‑1 (MQ=60)
                                                                                                                                   AAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATATTTTTCCGCGAGTCGAGTAAAAATCCGGTGAGCATATCGGTAAACGCGGTA               >  SRR19973065.2326610/1‑150 (MQ=60)
                                                                                                                                    AACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATATTTTTCCGCGAGTCGAGTCAAAATCCGGTGAGCATATCGGTAAACGCGGTAA              >  SRR19973065.2096540/1‑150 (MQ=60)
                                                                                                                                     ACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATATTTTTCCGCGAGTCGAGTAAAAATCCGGTGAGCATATCGGTAAACGCGGTAAA             <  SRR19973065.120840/150‑1 (MQ=60)
                                                                                                                                     ACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATATTTTTCCGCGAGTCGAGTAAAAATCCGGTGAGCATATCGGTAAACGCGGTAAA             <  SRR19973065.1907435/150‑1 (MQ=60)
                                                                                                                                         AAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATATTTTTCCGCGAGTCGAGTAAAAATCCGGTGAGCATATCGGTAAACGCGGTAAAAAAC         <  SRR19973065.1688310/150‑1 (MQ=60)
                                                                                                                                         AAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATATTTTTCCGCGAGTCGAGTAAAAATCCGGTGAGCATATCGGTAAACGCGGTAAAAAAC         <  SRR19973065.1689782/150‑1 (MQ=60)
                                                                                                                                              GGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATATTTTTCCGCGAGTCGAGTAAAAATCCGGTGAGCATATCGGTAAACGCGGTAAAAAACTTCGC    >  SRR19973065.1253020/1‑150 (MQ=60)
                                                                                                                                                TGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATATTTTTCCGCGAGTCGAGTAAAAATCCGGTGAGCATATCGGTAAACGCGGTAAAAAACTTCGCCA  <  SRR19973065.2261477/150‑1 (MQ=60)
                                                                                                                                                    |                                                                                                                                                 
CCGCGAGCTGCGCGCGCTCATTCGGGTTTTTGGTAATTGCCGGGATCATCGCACCATACGAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCGATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATATTTTTCCGCGAGTCGAGTAAAAATCCGGTGAGCATATCGGTAAACGCGGTAAAAAACTTCGCCA  >  CP009273/4058661‑4058954

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑